Rh7BG287600

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009135
Physical Location & Seq
Reverse (-)
27018949 .. 27025522
6574 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG287600.1

Sequence Viewer

Length: 1578 bp
ATGTTTATGTTTGCTTTCTGTTTGGCAGTGCTTGTTTCGAGGCAGTTTATGGATATGTCTCGTATAAGGATTGAGGGTCTTCTTGCGGCTTTCCCTAAGTTGGTAGGCACTGGAAAACAACATACATATGTCGAGACGGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTATTTGCTTCTAGTTACAAACAAGCAGAGCAATATCTTAGAAGATTTGGAGACTTTGAGGCTGCTCTCTAAGCTTGTACCTGAATATGCATTGTCCCTAGATGAAGAGGGTATATGCAGGACAGCTTTCGAGTTGATTTTTGCTTTTGATGAAGTAATCTCTCTTGGGCACAAGGAGAATGTGACTGTTGCACAGGTGAAGCAGTACTGTGAGATGGAGAGTCATGAAGAGAAGCTTCACAAGCTCGTAATGCAGAGCAAGATAAATGAGACCAAGGATGTTATGAAGCGTAAAGCCAGTGAGATTGACAAAAGCAAGATTGATAGGAGCAGAGGTGAAAAGGGAGGATTTGGGCCTTTGCAGTCAATGGGTTCTGGAAGAATTGAGAGTGCCTTTAGTGAGATGAGCTTATCCAGCACTGGAAGTGGTTTTGGAAGTGGTTCTGGTTTGGGAGCGACCGCTGATTTTGAAACATTTTCTTCGAAGGCCAAAGGCCGTCCACCTTCATCTGCCACAGCTCCGCCAAAGGGTTTAGGCATGCAGCTTGGAAAATCACAAAGGACCAACCAATTTTTAGAGTCCTTAAAAGCGGAGGGAGAGGTGATTCTTGAAGATGTACAGCCAAAAGCAGGCCAGTCAAAGTCGGTTGCCCCACCTTTAACTGATCCCGTCACTTTGAATGTCGAAGAGAAACTTAATGTAACACTTAAACGTGATGGTGGAGTCAGTAACTTTGATGTTCAAGGAACATTATCCCTGCAAATTCTTAACCAAGAAGACGGGCATATCCAAGTCCAGATTGAAACCGGTGGAAATCCTGGCATCCTTTTTAAAACTCATCCTAACATGAACAAAGAACTATTTTCTAATGAAAATATTCTAGGCTTGAAAGATCCTAATAGGCCTTTCCCCACTGGTCAAGCTGGTGATGCAGCAGGTGTTGGTCTTTTGAAATGGAGAATGCAGAGTCAAGATGAATCATTGGTGCCTCTACAAATCAACTGTTGGCCCTCTGTTTCTGGAAATGAAACCTATGTCAACATTGAGTATGATGCTTCATCAATGTTCGATCTGCGAAATGTTGTCATTTCAGTACCTCTTCCAGCTCTCAGAGAGGCTCCAGTTGTGAAGGAGATTCATGGTGAATGGAGATATGATGCAAGGAATTCAGTATTGGAGTGGTCTATACTTCTTATTGATAACTCAAACCGCAGTGGATCAATGGAGTTTGTTGTTCCTCCAGCAGACTCGAATGTATTCTTTCCCATTTCTGTGCGCTTCTCTGCCACTAGTACATTCAGTGACCTGAAGGTTGTGAATATCTTACCTCTGAAAGGTGGAGCCCCTCCCAAGTTCGCTCAGAGAACACAGTTGCTCACAGAGAACTACCAGGTGGTGTAA

Protein Analysis

525

Amino Acids

57.86

Weight (kDa)

5.53

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 280 - 509 2e-43 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1103
Acc36I ACCTGC 1 cut(s) 1103
AccB1I GGYRCC 1 cut(s) 1162
AciI CCGC 5 cut(s) 86, 636, 698, 767, 1387
AclWI GGATC 3 cut(s) 836, 1064, 1402
AcsI RAATTY 2 cut(s) 939, 1342
AcuI CTGAAG 1 cut(s) 1505
AfaI GTAC 5 cut(s) 255, 383, 795, 1272, 1471
AfiI CCNNNNNNNGG 4 cut(s) 100, 704, 806, 1511
AgeI ACCGGT 1 cut(s) 983
AgsI TTSAA 7 cut(s) 647, 788, 856, 920, 980, 1066, 1129
AhlI ACTAGT 1 cut(s) 1466
AjnI CCWGG 2 cut(s) 994, 1566
AjuI GAANNNNNNNTTGG 2 cut(s) 1334, 1366
Alw26I GTCTC 4 cut(s) 63, 128, 221, 440
AlwI GGATC 3 cut(s) 836, 1064, 1402
AoxI GGCC 6 cut(s) 530, 663, 670, 808, 1079, 1184
ApeKI GCWGC 3 cut(s) 238, 718, 1109
ApoI RAATTY 2 cut(s) 939, 1342
AsiGI ACCGGT 1 cut(s) 983
Asp700I GAANNNNTTC 3 cut(s) 616, 1053, 1433
AspLEI GCGC 2 cut(s) 150, 1455
AspS9I GGNCC 3 cut(s) 530, 738, 1185
AsuHPI GGTGA 5 cut(s) 385, 524, 790, 1115, 1331
AsuII TTCGAA 1 cut(s) 659
AvaII GGWCC 1 cut(s) 738
BaeGI GKGCMC 1 cut(s) 348
BanI GGYRCC 1 cut(s) 1162
BanII GRGCYC 1 cut(s) 1522
BbsI GAAGAC 2 cut(s) 71, 960
BbvI GCAGC 3 cut(s) 225, 730, 1121
BccI CCATC 2 cut(s) 385, 887
BceAI ACGGC 1 cut(s) 657
BciT130I CCWGG 2 cut(s) 996, 1568
BcoDI GTCTC 4 cut(s) 63, 128, 221, 440
BcuI ACTAGT 1 cut(s) 1466
BfaI CTAG 4 cut(s) 188, 275, 1058, 1467
BfuAI ACCTGC 1 cut(s) 1103
BisI GCNGC 4 cut(s) 87, 239, 719, 1110
BlsI GCNGC 4 cut(s) 88, 240, 720, 1111
BmcAI AGTACT 1 cut(s) 383
Bme1390I CCNGG 2 cut(s) 996, 1568
Bme18I GGWCC 1 cut(s) 738
BmgT120I GGNCC 3 cut(s) 530, 738, 1185
BmiI GGNNCC 3 cut(s) 1164, 1296, 1519
BmrFI CCNGG 2 cut(s) 996, 1568
BmsI GCATC 4 cut(s) 1008, 1096, 1219, 1324
BpiI GAAGAC 2 cut(s) 71, 960
BpmI CTGGAG 2 cut(s) 1281, 1401
Bpu14I TTCGAA 1 cut(s) 659
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 1 cut(s) 450
BsaWI WCCGGW 1 cut(s) 983
BsaXI ACNNNNNCTCC 2 cut(s) 496, 526
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 704, 806, 1511
Bse118I RCCGGY 1 cut(s) 983
Bse1I ACTGG 6 cut(s) 115, 474, 601, 811, 1096, 1298
BseBI CCWGG 2 cut(s) 996, 1568
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 3 cut(s) 460, 999, 1015
BseLI CCNNNNNNNGG 4 cut(s) 100, 704, 806, 1511
BseMII CTCAG 2 cut(s) 1300, 1550
BseNI ACTGG 6 cut(s) 115, 474, 601, 811, 1096, 1298
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 3 cut(s) 225, 730, 1121
Bsh1285I CGRYCG 1 cut(s) 636
BshFI GGCC 6 cut(s) 532, 665, 672, 810, 1081, 1186
BshNI GGYRCC 1 cut(s) 1162
BshTI ACCGGT 1 cut(s) 983
BsiEI CGRYCG 1 cut(s) 636
BsiSI CCGG 1 cut(s) 984
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 4 cut(s) 100, 704, 806, 1511
BsmAI GTCTC 4 cut(s) 63, 128, 221, 440
BsmBI CGTCTC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 256
BsmI GAATGC 1 cut(s) 1143
BsnI GGCC 6 cut(s) 532, 665, 672, 810, 1081, 1186
Bso31I GGTCTC 1 cut(s) 440
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 348, 1522
Bsp1407I TGTACA 1 cut(s) 793
Bsp143I GATC 4 cut(s) 841, 1069, 1246, 1394
BspACI CCGC 5 cut(s) 86, 636, 698, 767, 1387
BspANI GGCC 6 cut(s) 532, 665, 672, 810, 1081, 1186
BspCNI CTCAG 2 cut(s) 1299, 1549
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 1164, 1296, 1519
BspMI ACCTGC 1 cut(s) 1103
BspPI GGATC 3 cut(s) 836, 1064, 1402
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 1162
BspTNI GGTCTC 1 cut(s) 440
BsrFI RCCGGY 1 cut(s) 983
BsrGI TGTACA 1 cut(s) 793
BsrI ACTGG 6 cut(s) 115, 474, 601, 811, 1096, 1298
BssAI RCCGGY 1 cut(s) 983
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 4 cut(s) 841, 1069, 1246, 1394
BssT1I CCWWGG 1 cut(s) 450
Bst2UI CCWGG 2 cut(s) 996, 1568
Bst4CI ACNGT 4 cut(s) 364, 386, 1181, 1548
Bst6I CTCTTC 4 cut(s) 276, 399, 858, 1281
BstAUI TGTACA 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 716, 808
BstDEI CTNAG 5 cut(s) 96, 214, 246, 1286, 1536
BstENI CCTNNNNNAGG 1 cut(s) 1509
BstF5I GGATG 3 cut(s) 460, 999, 1015
BstHHI GCGC 2 cut(s) 150, 1455
BstKTI GATC 4 cut(s) 844, 1072, 1249, 1397
BstMAI GTCTC 4 cut(s) 63, 128, 221, 440
BstMBI GATC 4 cut(s) 841, 1069, 1246, 1394
BstMCI CGRYCG 1 cut(s) 636
BstMWI GCNNNNNNNGC 5 cut(s) 247, 418, 427, 591, 1106
BstNI CCWGG 2 cut(s) 996, 1568
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 994, 1566
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 3 cut(s) 225, 730, 1121
BstV2I GAAGAC 2 cut(s) 71, 960
BstX2I RGATCY 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 1069
BsuRI GGCC 6 cut(s) 532, 665, 672, 810, 1081, 1186
BtsCI GGATG 3 cut(s) 460, 999, 1015
BtsI GCAGTG 2 cut(s) 33, 1396
BtsIMutI CAGTG 7 cut(s) 33, 108, 481, 594, 1089, 1396, 1483
BveI ACCTGC 1 cut(s) 1103
Cac8I GCNNGC 2 cut(s) 716, 808
CciI TCATGA 1 cut(s) 400
CfoI GCGC 2 cut(s) 150, 1455
Cfr10I RCCGGY 1 cut(s) 983
Cfr13I GGNCC 3 cut(s) 530, 738, 1185
CsiI ACCWGGT 1 cut(s) 1566
Csp6I GTAC 5 cut(s) 254, 382, 794, 1271, 1470
CspAI ACCGGT 1 cut(s) 983
CviAII CATG 4 cut(s) 401, 715, 1024, 1316
CviQI GTAC 5 cut(s) 254, 382, 794, 1271, 1470
DdeI CTNAG 5 cut(s) 96, 214, 246, 1286, 1536
DpnI GATC 4 cut(s) 843, 1071, 1248, 1396
DpnII GATC 4 cut(s) 841, 1069, 1246, 1394
DraI TTTAAA 1 cut(s) 1009
Eam1104I CTCTTC 4 cut(s) 276, 399, 858, 1281
EarI CTCTTC 4 cut(s) 276, 399, 858, 1281
EciI GGCGGA 1 cut(s) 687
Eco130I CCWWGG 1 cut(s) 450
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 1 cut(s) 1522
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 738
Eco57I CTGAAG 1 cut(s) 1505
EcoNI CCTNNNNNAGG 1 cut(s) 1509
EcoRI GAATTC 1 cut(s) 1342
EcoRII CCWGG 2 cut(s) 994, 1566
EcoT14I CCWWGG 1 cut(s) 450
EcoT22I ATGCAT 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 1522
ErhI CCWWGG 1 cut(s) 450
Esp3I CGTCTC 1 cut(s) 128
FaeI CATG 4 cut(s) 404, 718, 1027, 1319
FalI AAGNNNNNCTT 4 cut(s) 396, 428, 855, 887
FaqI GGGAC 1 cut(s) 256
FatI CATG 4 cut(s) 400, 714, 1023, 1315
FauNDI CATATG 1 cut(s) 127
Fnu4HI GCNGC 4 cut(s) 87, 239, 719, 1110
FokI GGATG 3 cut(s) 467, 986, 1002
FriOI GRGCYC 1 cut(s) 1522
Fsp4HI GCNGC 4 cut(s) 87, 239, 719, 1110
FspBI CTAG 4 cut(s) 188, 275, 1058, 1467
GlaI GCGC 2 cut(s) 149, 1454
GluI GCNGC 4 cut(s) 87, 239, 719, 1110
GsuI CTGGAG 2 cut(s) 1281, 1401
HaeIII GGCC 6 cut(s) 532, 665, 672, 810, 1081, 1186
HapII CCGG 1 cut(s) 984
HhaI GCGC 2 cut(s) 150, 1455
Hin1II CATG 4 cut(s) 404, 718, 1027, 1319
Hin6I GCGC 2 cut(s) 148, 1453
HinP1I GCGC 2 cut(s) 148, 1453
HincII GTYRAC 1 cut(s) 1216
HindII GTYRAC 1 cut(s) 1216
HindIII AAGCTT 3 cut(s) 171, 248, 410
HinfI GANTC 8 cut(s) 397, 755, 781, 900, 1144, 1154, 1312, 1424
HpaII CCGG 1 cut(s) 984
HphI GGTGA 5 cut(s) 385, 524, 790, 1115, 1331
Hpy166II GTNNAC 3 cut(s) 157, 677, 1216
Hpy188I TCNGA 3 cut(s) 1289, 1509, 1539
Hpy188III TCNNGA 7 cut(s) 133, 401, 552, 785, 973, 1148, 1197
Hpy8I GTNNAC 3 cut(s) 157, 677, 1216
HpyAV CCTTC 4 cut(s) 655, 690, 1300, 1480
HpyCH4III ACNGT 4 cut(s) 364, 386, 1181, 1548
HpyCH4IV ACGT 1 cut(s) 889
HpyF10VI GCNNNNNNNGC 5 cut(s) 247, 418, 427, 591, 1106
HpyF3I CTNAG 5 cut(s) 96, 214, 246, 1286, 1536
HpySE526I ACGT 1 cut(s) 889
Hsp92II CATG 4 cut(s) 404, 718, 1027, 1319
HspAI GCGC 2 cut(s) 148, 1453
Kzo9I GATC 4 cut(s) 841, 1069, 1246, 1394
LmnI GCTCC 5 cut(s) 504, 629, 700, 1300, 1517
Lsp1109I GCAGC 3 cut(s) 225, 730, 1121
LweI GCATC 4 cut(s) 1008, 1096, 1219, 1324
MabI ACCWGGT 1 cut(s) 1566
MaeI CTAG 4 cut(s) 188, 275, 1058, 1467
MaeII ACGT 1 cut(s) 889
MaeIII GTNAC 6 cut(s) 190, 358, 847, 877, 905, 1478
MalI GATC 4 cut(s) 843, 1071, 1248, 1396
MboI GATC 4 cut(s) 841, 1069, 1246, 1394
MflI RGATCY 1 cut(s) 1069
MhlI GDGCHC 2 cut(s) 348, 1522
MluCI AATT 4 cut(s) 558, 746, 939, 1342
MlyI GAGTC 5 cut(s) 406, 764, 909, 1153, 1418
Mph1103I ATGCAT 1 cut(s) 268
MroXI GAANNNNTTC 3 cut(s) 616, 1053, 1433
MseI TTAA 6 cut(s) 761, 836, 873, 885, 945, 1008
MslI CAYNNNNRTG 2 cut(s) 126, 1448
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 1 cut(s) 984
MspR9I CCNGG 2 cut(s) 996, 1568
Mva1269I GAATGC 1 cut(s) 1143
MvaI CCWGG 2 cut(s) 996, 1568
MwoI GCNNNNNNNGC 5 cut(s) 247, 418, 427, 591, 1106
NdeI CATATG 1 cut(s) 127
NdeII GATC 4 cut(s) 841, 1069, 1246, 1394
NlaIII CATG 4 cut(s) 404, 718, 1027, 1319
NlaIV GGNNCC 3 cut(s) 1164, 1296, 1519
NmuCI GTSAC 3 cut(s) 358, 847, 1478
NsiI ATGCAT 1 cut(s) 268
NspI RCATGY 1 cut(s) 718
NspV TTCGAA 1 cut(s) 659
PaeI GCATGC 1 cut(s) 718
PagI TCATGA 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 1103
PceI AGGCCT 1 cut(s) 1081
PctI GAATGC 1 cut(s) 1143
PdmI GAANNNNTTC 3 cut(s) 616, 1053, 1433
PfeI GAWTC 3 cut(s) 781, 1154, 1312
PinAI ACCGGT 1 cut(s) 983
PkrI GCNGC 4 cut(s) 88, 240, 720, 1111
PleI GAGTC 5 cut(s) 405, 763, 908, 1152, 1418
PpsI GAGTC 5 cut(s) 405, 763, 908, 1152, 1418
Psp6I CCWGG 2 cut(s) 994, 1566
PspGI CCWGG 2 cut(s) 994, 1566
PspN4I GGNNCC 3 cut(s) 1164, 1296, 1519
PspPI GGNCC 3 cut(s) 530, 738, 1185
PsuI RGATCY 1 cut(s) 1069
RsaI GTAC 5 cut(s) 255, 383, 795, 1272, 1471
RsaNI GTAC 5 cut(s) 254, 382, 794, 1271, 1470
RseI CAYNNNNRTG 2 cut(s) 126, 1448
SaqAI TTAA 6 cut(s) 761, 836, 873, 885, 945, 1008
SatI GCNGC 4 cut(s) 87, 239, 719, 1110
Sau3AI GATC 4 cut(s) 841, 1069, 1246, 1394
Sau96I GGNCC 3 cut(s) 530, 738, 1185
ScaI AGTACT 1 cut(s) 383
SchI GAGTC 5 cut(s) 406, 764, 909, 1153, 1418
ScrFI CCNGG 2 cut(s) 996, 1568
SduI GDGCHC 2 cut(s) 348, 1522
SexAI ACCWGGT 1 cut(s) 1566
SfaNI GCATC 4 cut(s) 1008, 1096, 1219, 1324
SfuI TTCGAA 1 cut(s) 659
SinI GGWCC 1 cut(s) 738
SmiMI CAYNNNNRTG 2 cut(s) 126, 1448
SpeI ACTAGT 1 cut(s) 1466
SphI GCATGC 1 cut(s) 718
Sse9I AATT 4 cut(s) 558, 746, 939, 1342
SseBI AGGCCT 1 cut(s) 1081
SsiI CCGC 5 cut(s) 86, 636, 698, 767, 1387
SspI AATATT 1 cut(s) 1054
SspMI CTAG 4 cut(s) 188, 275, 1058, 1467
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 2 cut(s) 994, 1566
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 4 cut(s) 364, 386, 1181, 1548
TaiI ACGT 1 cut(s) 892
TaqI TCGA 7 cut(s) 38, 132, 306, 659, 861, 1245, 1427
TasI AATT 4 cut(s) 558, 746, 939, 1342
TatI WGTACW 3 cut(s) 381, 793, 1469
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 3 cut(s) 781, 1154, 1312
Tru1I TTAA 6 cut(s) 761, 836, 873, 885, 945, 1008
Tru9I TTAA 6 cut(s) 761, 836, 873, 885, 945, 1008
TscAI CASTG 7 cut(s) 33, 115, 481, 601, 1096, 1396, 1483
TseFI GTSAC 3 cut(s) 358, 847, 1478
TseI GCWGC 3 cut(s) 238, 718, 1109
Tsp45I GTSAC 3 cut(s) 358, 847, 1478
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 7 cut(s) 33, 115, 481, 601, 1096, 1396, 1483
VpaK11BI GGWCC 1 cut(s) 738
XagI CCTNNNNNAGG 1 cut(s) 1509
XapI RAATTY 2 cut(s) 939, 1342
XceI RCATGY 1 cut(s) 718
XmnI GAANNNNTTC 3 cut(s) 616, 1053, 1433
XspI CTAG 4 cut(s) 188, 275, 1058, 1467
ZrmI AGTACT 1 cut(s) 383
Zsp2I ATGCAT 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.