Rorug07G0158300

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009135
Physical Location & Seq
Forward (+)
12590863 .. 12593303
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0158300.1

Sequence Viewer

Length: 879 bp
ATGGCCACCGGAGTTTCGGTTGCCTCAATCCCAATATGGTACTGTTTCGGCCGAAACAATCTACTTGCTCGAAAGCCCATCTCGCAAACAAGCACCCCCTGTTTTGTTTTGGCTTCAGCAAAGAAGAGACACTTGGTTATGGAAAAGAGAACAGAGCATGAATTTACAGAGAAAAGCTTGGAACAAACAGAGAGAAGGAGTGTTATAACTCCAAGAGTTGCAGAGAGATTAGCAAGAAGGAAATCGGAGAGAAATACTTACCTTGTTGCTGCAATCATGTCCAGCCTGGGAGTCACTTCAGCTGTTGCCATGGCTGTTTACTACAGATTTTCTTGGCAAACAGAGATTGGGGAGTTTCTTGTTCCAGAGATGTTGGGCACATTTTTGCTCTCTGTGGGCTCTGCTGTGGGGATGGAGTTTTGGGCAAGGTGGGCGCACAGAGCTCTGTGGCATGCCTCATTATGGCAGATGCATGAGTCTCACCATCGACCTAGGGATGGTCCTTTCGAGATTAACGACGTGTTTGCTATAACCAATGCTGCTCCTGCTATTGCTCTTCTCTCTTACGGTTTCTTTACCAAGGGTCTTTTTCCTGGACTATGTTTTGGAGCTGGGCTAGGGATCACAGTTTTCGGGATGGCATACATGTTTGTGCACGATGGTCTCGTCCACCGTCGATTTCCAGTCGGGCCAATCGCAGACGTCCCTTATTTACAAAGAGTTGCTGCCGCCCACCAGGTTCATCATACATACATATTGAATGGAGTGCCGTATGGGTTGTTCTTGGGACCTAAGGAAATAGAAGAGGTTGGAGGCATGGAAGAATTAGAAAAGGAGATCAAGCGGAGAAGCACCAAGAGTACTATACCCAAAAGCTGA

Protein Analysis

292

Amino Acids

32.78

Weight (kDa)

9.46

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 135 - 261 2.2e-14 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AatII GACGTC 1 cut(s) 705
AciI CCGC 2 cut(s) 729, 844
AclWI GGATC 1 cut(s) 629
AcoI YGGCCR 2 cut(s) 3, 49
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 2 cut(s) 99, 282
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 2 cut(s) 41, 862
AfiI CCNNNNNNNGG 2 cut(s) 462, 497
AflIII ACRYGT 2 cut(s) 519, 645
AgsI TTSAA 1 cut(s) 760
AjiI CACGTC 1 cut(s) 520
AjnI CCWGG 3 cut(s) 285, 592, 735
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AluBI AGCT 5 cut(s) 177, 302, 443, 611, 876
AluI AGCT 5 cut(s) 177, 302, 443, 611, 876
Alw21I GWGCWC 2 cut(s) 445, 657
Alw26I GTCTC 3 cut(s) 121, 483, 668
Alw44I GTGCAC 1 cut(s) 653
AlwI GGATC 1 cut(s) 629
AoxI GGCC 3 cut(s) 3, 49, 689
ApaLI GTGCAC 1 cut(s) 653
ApeKI GCWGC 3 cut(s) 269, 539, 725
ApoI RAATTY 1 cut(s) 161
AspA2I CCTAGG 1 cut(s) 491
AspLEI GCGC 1 cut(s) 436
AspS9I GGNCC 3 cut(s) 500, 689, 788
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 2 cut(s) 500, 788
AvrII CCTAGG 1 cut(s) 491
AxyI CCTNAGG 1 cut(s) 792
BaeGI GKGCMC 2 cut(s) 380, 657
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 2 cut(s) 401, 445
Bbv12I GWGCWC 2 cut(s) 445, 657
BbvI GCAGC 3 cut(s) 256, 526, 712
BccI CCATC 6 cut(s) 86, 406, 491, 492, 631, 653
BceAI ACGGC 1 cut(s) 754
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 3 cut(s) 287, 594, 737
BcoDI GTCTC 3 cut(s) 121, 483, 668
BfaI CTAG 2 cut(s) 492, 617
BfmI CTRYAG 1 cut(s) 322
BisI GCNGC 4 cut(s) 270, 540, 726, 729
BlnI CCTAGG 1 cut(s) 491
BlsI GCNGC 4 cut(s) 271, 541, 727, 730
BmcAI AGTACT 1 cut(s) 862
Bme1390I CCNGG 3 cut(s) 287, 594, 737
Bme18I GGWCC 2 cut(s) 500, 788
BmgBI CACGTC 1 cut(s) 520
BmgT120I GGNCC 3 cut(s) 500, 689, 788
BmiI GGNNCC 1 cut(s) 789
BmrFI CCNGG 3 cut(s) 287, 594, 737
BmsI GCATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 702
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 4 cut(s) 286, 309, 491, 579
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 497
Bse1I ACTGG 1 cut(s) 683
Bse21I CCTNAGG 1 cut(s) 792
BseBI CCWGG 3 cut(s) 287, 594, 737
BseDI CCNNGG 4 cut(s) 286, 309, 491, 579
BseGI GGATG 3 cut(s) 417, 502, 642
BseLI CCNNNNNNNGG 2 cut(s) 462, 497
BseNI ACTGG 1 cut(s) 683
BseSI GKGCMC 2 cut(s) 380, 657
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 256, 526, 712
BseYI CCCAGC 1 cut(s) 611
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 3 cut(s) 5, 51, 691
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 2 cut(s) 445, 657
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 2 cut(s) 689, 801
BslI CCNNNNNNNGG 2 cut(s) 462, 497
BsmAI GTCTC 3 cut(s) 121, 483, 668
BsmFI GGGAC 2 cut(s) 689, 801
BsnI GGCC 3 cut(s) 5, 51, 691
Bso31I GGTCTC 1 cut(s) 668
Bsp1286I GDGCHC 4 cut(s) 380, 401, 445, 657
Bsp143I GATC 2 cut(s) 621, 837
Bsp19I CCATGG 1 cut(s) 309
BspACI CCGC 2 cut(s) 729, 844
BspANI GGCC 3 cut(s) 5, 51, 691
BspLI GGNNCC 1 cut(s) 789
BspPI GGATC 1 cut(s) 629
BspQI GCTCTTC 1 cut(s) 561
BspTNI GGTCTC 1 cut(s) 668
BsrI ACTGG 1 cut(s) 683
BssECI CCNNGG 4 cut(s) 286, 309, 491, 579
BssMI GATC 2 cut(s) 621, 837
BssNI GRCGYC 1 cut(s) 702
BssT1I CCWWGG 3 cut(s) 309, 491, 579
Bst2UI CCWGG 3 cut(s) 287, 594, 737
Bst4CI ACNGT 4 cut(s) 44, 569, 628, 674
Bst6I CTCTTC 3 cut(s) 119, 561, 798
BstACI GRCGYC 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 1 cut(s) 792
BstDSI CCRYGG 1 cut(s) 309
BstF5I GGATG 3 cut(s) 417, 502, 642
BstHHI GCGC 1 cut(s) 436
BstKTI GATC 2 cut(s) 624, 840
BstMAI GTCTC 3 cut(s) 121, 483, 668
BstMBI GATC 2 cut(s) 621, 837
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 4 cut(s) 82, 431, 440, 545
BstNI CCWGG 3 cut(s) 287, 594, 737
BstNSI RCATGY 2 cut(s) 455, 649
BstSCI CCNGG 3 cut(s) 285, 592, 735
BstSFI CTRYAG 1 cut(s) 322
BstSLI GKGCMC 2 cut(s) 380, 657
BstV1I GCAGC 3 cut(s) 256, 526, 712
BstZI CGGCCG 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 3 cut(s) 5, 51, 691
BtgI CCRYGG 1 cut(s) 309
BtrI CACGTC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 417, 502, 642
Cac8I GCNNGC 1 cut(s) 453
CfoI GCGC 1 cut(s) 436
Cfr13I GGNCC 3 cut(s) 500, 689, 788
CsiI ACCWGGT 1 cut(s) 735
Csp6I GTAC 2 cut(s) 40, 861
CviAII CATG 7 cut(s) 158, 277, 310, 452, 473, 646, 817
CviQI GTAC 2 cut(s) 40, 861
DdeI CTNAG 1 cut(s) 792
DpnI GATC 2 cut(s) 623, 839
DpnII GATC 2 cut(s) 621, 837
EaeI YGGCCR 2 cut(s) 3, 49
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 3 cut(s) 119, 561, 798
EarI CTCTTC 3 cut(s) 119, 561, 798
Ecl136II GAGCTC 1 cut(s) 443
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 309, 491, 579
Eco24I GRGCYC 2 cut(s) 401, 445
Eco31I GGTCTC 1 cut(s) 668
Eco47I GGWCC 2 cut(s) 500, 788
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 443
Eco57I CTGAAG 2 cut(s) 99, 282
Eco81I CCTNAGG 1 cut(s) 792
EcoICRI GAGCTC 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 788
EcoRII CCWGG 3 cut(s) 285, 592, 735
EcoT14I CCWWGG 3 cut(s) 309, 491, 579
EcoT22I ATGCAT 1 cut(s) 474
EcoT38I GRGCYC 2 cut(s) 401, 445
ErhI CCWWGG 3 cut(s) 309, 491, 579
FaeI CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
FalI AAGNNNNNCTT 2 cut(s) 116, 148
FaqI GGGAC 2 cut(s) 689, 801
FatI CATG 7 cut(s) 157, 276, 309, 451, 472, 645, 816
Fnu4HI GCNGC 4 cut(s) 270, 540, 726, 729
FokI GGATG 3 cut(s) 424, 509, 649
FriOI GRGCYC 2 cut(s) 401, 445
Fsp4HI GCNGC 4 cut(s) 270, 540, 726, 729
FspBI CTAG 2 cut(s) 492, 617
GlaI GCGC 1 cut(s) 435
GluI GCNGC 4 cut(s) 270, 540, 726, 729
GsaI CCCAGC 1 cut(s) 615
HaeIII GGCC 3 cut(s) 5, 51, 691
HapII CCGG 1 cut(s) 9
HhaI GCGC 1 cut(s) 436
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
Hin6I GCGC 1 cut(s) 434
HinP1I GCGC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 175
HinfI GANTC 2 cut(s) 291, 476
HpaII CCGG 1 cut(s) 9
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 3 cut(s) 319, 655, 670
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 3 cut(s) 365, 508, 634
Hpy8I GTNNAC 3 cut(s) 319, 655, 670
Hpy99I CGWCG 2 cut(s) 521, 678
HpyAV CCTTC 2 cut(s) 189, 231
HpyCH4III ACNGT 4 cut(s) 44, 569, 628, 674
HpyCH4IV ACGT 2 cut(s) 519, 702
HpyCH4V TGCA 4 cut(s) 221, 272, 472, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 82, 431, 440, 545
HpyF3I CTNAG 1 cut(s) 792
HpySE526I ACGT 2 cut(s) 519, 702
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
HspAI GCGC 1 cut(s) 434
Kzo9I GATC 2 cut(s) 621, 837
LguI GCTCTTC 1 cut(s) 561
LmnI GCTCC 2 cut(s) 547, 608
Lsp1109I GCAGC 3 cut(s) 256, 526, 712
LweI GCATC 1 cut(s) 459
MabI ACCWGGT 1 cut(s) 735
MaeI CTAG 2 cut(s) 492, 617
MaeII ACGT 2 cut(s) 519, 702
MaeIII GTNAC 1 cut(s) 292
MalI GATC 2 cut(s) 623, 839
MboI GATC 2 cut(s) 621, 837
MboII GAAGA 4 cut(s) 136, 548, 815, 833
MhlI GDGCHC 4 cut(s) 380, 401, 445, 657
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 161, 824
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 300, 485
MmeI TCCRAC 1 cut(s) 790
MnlI CCTC 4 cut(s) 34, 466, 799, 806
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 474
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 513
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 3 cut(s) 287, 594, 737
MvaI CCWGG 3 cut(s) 287, 594, 737
MwoI GCNNNNNNNGC 4 cut(s) 82, 431, 440, 545
NcoI CCATGG 1 cut(s) 309
NdeII GATC 2 cut(s) 621, 837
NlaIII CATG 7 cut(s) 161, 280, 313, 455, 476, 649, 820
NlaIV GGNNCC 1 cut(s) 789
NmuCI GTSAC 1 cut(s) 292
NsiI ATGCAT 1 cut(s) 474
NspI RCATGY 2 cut(s) 455, 649
PaeI GCATGC 1 cut(s) 455
PciI ACATGT 1 cut(s) 645
PciSI GCTCTTC 1 cut(s) 561
PcsI WCGNNNNNNNCGW 2 cut(s) 513, 663
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 4 cut(s) 271, 541, 727, 730
PleI GAGTC 2 cut(s) 299, 484
PpsI GAGTC 2 cut(s) 299, 484
PpuMI RGGWCCY 1 cut(s) 788
PscI ACATGT 1 cut(s) 645
PsiI TTATAA 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 445
Psp5II RGGWCCY 1 cut(s) 788
Psp6I CCWGG 3 cut(s) 285, 592, 735
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 285, 592, 735
PspN4I GGNNCC 1 cut(s) 789
PspPI GGNCC 3 cut(s) 500, 689, 788
PspPPI RGGWCCY 1 cut(s) 788
PsrI GAACNNNNNNTAC 2 cut(s) 764, 796
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 41, 862
RsaNI GTAC 2 cut(s) 40, 861
RseI CAYNNNNRTG 1 cut(s) 650
SacI GAGCTC 1 cut(s) 445
SapI GCTCTTC 1 cut(s) 561
SaqAI TTAA 1 cut(s) 513
SatI GCNGC 4 cut(s) 270, 540, 726, 729
Sau3AI GATC 2 cut(s) 621, 837
Sau96I GGNCC 3 cut(s) 500, 689, 788
ScaI AGTACT 1 cut(s) 862
SchI GAGTC 2 cut(s) 300, 485
ScrFI CCNGG 3 cut(s) 287, 594, 737
SduI GDGCHC 4 cut(s) 380, 401, 445, 657
SexAI ACCWGGT 1 cut(s) 735
SfaNI GCATC 1 cut(s) 459
SfcI CTRYAG 1 cut(s) 322
SinI GGWCC 2 cut(s) 500, 788
SmiMI CAYNNNNRTG 1 cut(s) 650
SphI GCATGC 1 cut(s) 455
Sse9I AATT 2 cut(s) 161, 824
SsiI CCGC 2 cut(s) 729, 844
SspMI CTAG 2 cut(s) 492, 617
SstI GAGCTC 1 cut(s) 445
StyD4I CCNGG 3 cut(s) 285, 592, 735
StyI CCWWGG 3 cut(s) 309, 491, 579
TaaI ACNGT 4 cut(s) 44, 569, 628, 674
TaiI ACGT 2 cut(s) 522, 705
TaqI TCGA 4 cut(s) 70, 487, 507, 676
TasI AATT 2 cut(s) 161, 824
TatI WGTACW 1 cut(s) 860
TauI GCSGC 1 cut(s) 731
Tru1I TTAA 1 cut(s) 513
Tru9I TTAA 1 cut(s) 513
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 3 cut(s) 269, 539, 725
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 174, 731
VneI GTGCAC 1 cut(s) 653
VpaK11BI GGWCC 2 cut(s) 500, 788
XapI RAATTY 1 cut(s) 161
XceI RCATGY 2 cut(s) 455, 649
XmaJI CCTAGG 1 cut(s) 491
XspI CTAG 2 cut(s) 492, 617
ZraI GACGTC 1 cut(s) 703
ZrmI AGTACT 1 cut(s) 862
Zsp2I ATGCAT 1 cut(s) 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.