FvH4_5g25700

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009135
Physical Location & Seq
Reverse (-)
17202543 .. 17209934
7392 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g25700.t1

Sequence Viewer

Length: 1593 bp
ATGGTTGTTCTTGCGGCTTCTGTTATAAACAAGTCTGGAAAAGTGCTTGTTTCCAGGCAGTTTATGGATATGTCCCGTATAAGGATCGAGGGTCTTCTTGCTGCTTTCCCTAAGTTGGTAGGCACTGGCAAACAACATACATATGTTGAGACAGAAAATGTGCGCTATGTTTACCAGCCAATAGAAGCTTTGTATCTGCTTCTAGTTACAAACAAGCAGAGCAATATCTTGGAAGATTTGGAGACTTTGAGGCTGCTCTCTAAGCTTGTACCTGAATATGCATTGTCCCTAGATGAAGATGGTATATGCAGGACAGCTTTTGAGTTGATTTTTGCTTTTGATGAAGTAATCTCTCTTGGGCACAAGGAGAACGTGACTGTTGCACAGGTGAAGCAGTACTGTGAGATGGAGAGTCATGAAGAGAAGCTTCACAAGCTTGTAATGCAGAGCAAGATAAATGAAACCAAGGATGTTATGAAGCGCAAGGCCAGTGAGATTGACAAAAGCAAGATTGATAGGAGCAGAGGTGAAAAGGGAGGATTTGGGCCTTTGCAGTCGATCGGTTCTGGAAGAATTGAGAGTGGCTTTAATGATATAAGCATATCCAGTAGTGGAAGTGGTTATGGAAGTGGTTCTGGTTTGGGACTGACCCCTGATTTTGAATCATTTTCTTCCAAGGCCAAAGGTCGTCCACCTTCATCTGCCACAGCTCCTCCAAAGGGTTCAGGCATGCAGCTTGGAAAATCACAAAGGACTAACCAATTTATAGAGTCCTTAAAAGCAGAGGGAGAGGTGATTCCTGAAGATGTTCAGCCAAAAGCAGGCCTGTCCAAATCTGCTGCCCCACCTTTAACTGATCCCGTCACTTTGAATGTTGAAGAGAAACTCAATGTAACACTCAAACGGGATGGTGGAGTCAGTAACTTTGATGTTCAAGGAACATTATCCCTGCAAATTCTTAACCAAGAAGATGGGCATATCCAAGTCCAGATTGAAACGGGTGGAAATCCTGGCATCCTTTTCAAAACGCACCCTAACATGAACAAAGAACTATTTTCTAATGAAAATATTCTAGGCCTGAAAGATCCTAACAGGCCTTTCCCCACTGGTCAAAGTGGTGATGCAGGAGGTGTTGGTCTTTTGAAGTGGAGAATGCAGAGTCAAGATGAATCGATGGTGCCTCTACAAATCAACTGTTGGCCCTCTGTTTCTGGAAATGAAACTTATGTCAACATTGAGTATGATGCTTCATCAATGTTTGATCTGCGGAATGTTGTCATCTCAGTACCTCTTCCAGCTCTCAGAGAGGCTCCAGTGGTGAAGGAGATTCATGGTGAATGGAGATATGATGCAAGGAATTCAGTATTGGAGTGGTCAATACTTCTTATTGATAATTCAAACCGCAGTGGATCAATGGAGTTTGTTGTTCCCCCAGCAGACTCGAATGTATTCTTTCCCATTTCTGTGCGCTTCTCTGCCACTAGTACATTCAGTGACCTAAAGGTTGTTAATATATTACCTCTGAAAGGTGGTGCTCCTCCCAAGTTTGCTCAGAGAACACAGTTGCTCACAGAGAACTACCAAGTTGTGTAA

Protein Analysis

531

Amino Acids

58.14

Weight (kDa)

5.72

Isoelectric Point (pI)

50.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 283 - 514 2.3e-44 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 1177
AciI CCGC 3 cut(s) 14, 1267, 1402
AclWI GGATC 4 cut(s) 92, 851, 1079, 1417
AcsI RAATTY 2 cut(s) 954, 1357
AcuI CTGAAG 1 cut(s) 822
AfaI GTAC 4 cut(s) 270, 398, 1287, 1486
AfiI CCNNNNNNNGG 5 cut(s) 81, 115, 719, 821, 1526
AgsI TTSAA 8 cut(s) 662, 871, 878, 935, 995, 1024, 1144, 1398
AhlI ACTAGT 1 cut(s) 1481
AjnI CCWGG 2 cut(s) 53, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 1349, 1381
AluBI AGCT 8 cut(s) 188, 265, 317, 427, 436, 710, 736, 1298
AluI AGCT 8 cut(s) 188, 265, 317, 427, 436, 710, 736, 1298
Alw21I GWGCWC 1 cut(s) 1537
Alw26I GTCTC 2 cut(s) 143, 236
AlwI GGATC 4 cut(s) 92, 851, 1079, 1417
AoxI GGCC 7 cut(s) 486, 545, 678, 823, 1075, 1094, 1199
ApeKI GCWGC 4 cut(s) 101, 253, 733, 839
ApoI RAATTY 2 cut(s) 954, 1357
Asp700I GAANNNNTTC 4 cut(s) 631, 807, 1068, 1448
AspLEI GCGC 3 cut(s) 165, 483, 1470
AspS9I GGNCC 2 cut(s) 545, 1200
AsuHPI GGTGA 6 cut(s) 400, 539, 805, 1130, 1330, 1346
BaeGI GKGCMC 1 cut(s) 363
BanI GGYRCC 1 cut(s) 1177
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 1537
BbvI GCAGC 4 cut(s) 88, 240, 745, 826
BccI CCATC 5 cut(s) 293, 400, 902, 965, 1168
BciT130I CCWGG 2 cut(s) 55, 1011
BcoDI GTCTC 2 cut(s) 143, 236
BcuI ACTAGT 1 cut(s) 1481
BfaI CTAG 4 cut(s) 203, 290, 1073, 1482
BisI GCNGC 5 cut(s) 15, 102, 254, 734, 840
BlsI GCNGC 5 cut(s) 16, 103, 255, 735, 841
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 55, 1011
BmgT120I GGNCC 2 cut(s) 545, 1200
BmiI GGNNCC 2 cut(s) 1179, 1311
BmrFI CCNGG 2 cut(s) 55, 1011
BmsI GCATC 4 cut(s) 1023, 1111, 1234, 1339
BpiI GAAGAC 1 cut(s) 86
BpmI CTGGAG 1 cut(s) 1296
Bsa29I ATCGAT 1 cut(s) 1172
BsaJI CCNNGG 2 cut(s) 465, 675
BsaXI ACNNNNNCTCC 4 cut(s) 511, 541, 697, 727
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 115, 719, 821, 1526
Bse1I ACTGG 5 cut(s) 130, 489, 606, 1111, 1313
BseBI CCWGG 2 cut(s) 55, 1011
BseCI ATCGAT 1 cut(s) 1172
BseDI CCNNGG 2 cut(s) 465, 675
BseGI GGATG 3 cut(s) 475, 913, 1014
BseLI CCNNNNNNNGG 5 cut(s) 81, 115, 719, 821, 1526
BseMII CTCAG 3 cut(s) 1296, 1315, 1565
BseNI ACTGG 5 cut(s) 130, 489, 606, 1111, 1313
BseRI GAGGAG 2 cut(s) 702, 1527
BseSI GKGCMC 1 cut(s) 363
BseXI GCAGC 4 cut(s) 88, 240, 745, 826
BseYI CCCAGC 1 cut(s) 1432
Bsh1285I CGRYCG 1 cut(s) 561
BshFI GGCC 7 cut(s) 488, 547, 680, 825, 1077, 1096, 1201
BshNI GGYRCC 1 cut(s) 1177
BshVI ATCGAT 1 cut(s) 1172
BsiEI CGRYCG 1 cut(s) 561
BsiHKAI GWGCWC 1 cut(s) 1537
BslFI GGGAC 3 cut(s) 58, 271, 657
BslI CCNNNNNNNGG 5 cut(s) 81, 115, 719, 821, 1526
BsmAI GTCTC 2 cut(s) 143, 236
BsmFI GGGAC 3 cut(s) 58, 271, 657
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 7 cut(s) 488, 547, 680, 825, 1077, 1096, 1201
Bsp1286I GDGCHC 2 cut(s) 363, 1537
Bsp143I GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
BspACI CCGC 3 cut(s) 14, 1267, 1402
BspANI GGCC 7 cut(s) 488, 547, 680, 825, 1077, 1096, 1201
BspCNI CTCAG 3 cut(s) 1295, 1314, 1564
BspDI ATCGAT 1 cut(s) 1172
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 2 cut(s) 1179, 1311
BspPI GGATC 4 cut(s) 92, 851, 1079, 1417
BspT107I GGYRCC 1 cut(s) 1177
BsrI ACTGG 5 cut(s) 130, 489, 606, 1111, 1313
BssECI CCNNGG 2 cut(s) 465, 675
BssMI GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
BssT1I CCWWGG 2 cut(s) 465, 675
Bst2UI CCWGG 2 cut(s) 55, 1011
Bst4CI ACNGT 4 cut(s) 379, 401, 1196, 1563
Bst6I CTCTTC 3 cut(s) 414, 873, 1296
BstC8I GCNNGC 2 cut(s) 731, 823
BstDEI CTNAG 5 cut(s) 111, 261, 1282, 1301, 1551
BstENI CCTNNNNNAGG 1 cut(s) 1524
BstF5I GGATG 3 cut(s) 475, 913, 1014
BstHHI GCGC 3 cut(s) 165, 483, 1470
BstKTI GATC 6 cut(s) 87, 561, 859, 1087, 1264, 1412
BstMAI GTCTC 2 cut(s) 143, 236
BstMBI GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
BstMCI CGRYCG 1 cut(s) 561
BstMWI GCNNNNNNNGC 3 cut(s) 262, 433, 442
BstNI CCWGG 2 cut(s) 55, 1011
BstNSI RCATGY 1 cut(s) 733
BstSCI CCNGG 2 cut(s) 53, 1009
BstSLI GKGCMC 1 cut(s) 363
BstV1I GCAGC 4 cut(s) 88, 240, 745, 826
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 1084
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 1 cut(s) 1084
Bsu15I ATCGAT 1 cut(s) 1172
BsuRI GGCC 7 cut(s) 488, 547, 680, 825, 1077, 1096, 1201
BsuTUI ATCGAT 1 cut(s) 1172
BtsCI GGATG 3 cut(s) 475, 913, 1014
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 6 cut(s) 123, 496, 1104, 1320, 1411, 1498
Cac8I GCNNGC 2 cut(s) 731, 823
CciI TCATGA 1 cut(s) 415
CfoI GCGC 3 cut(s) 165, 483, 1470
Cfr13I GGNCC 2 cut(s) 545, 1200
ClaI ATCGAT 1 cut(s) 1172
Csp6I GTAC 4 cut(s) 269, 397, 1286, 1485
CviAII CATG 4 cut(s) 416, 730, 1039, 1331
CviQI GTAC 4 cut(s) 269, 397, 1286, 1485
DdeI CTNAG 5 cut(s) 111, 261, 1282, 1301, 1551
DpnI GATC 6 cut(s) 86, 560, 858, 1086, 1263, 1411
DpnII GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
Eam1104I CTCTTC 3 cut(s) 414, 873, 1296
EarI CTCTTC 3 cut(s) 414, 873, 1296
Eco130I CCWWGG 2 cut(s) 465, 675
Eco147I AGGCCT 3 cut(s) 825, 1077, 1096
Eco57I CTGAAG 1 cut(s) 822
EcoNI CCTNNNNNAGG 1 cut(s) 1524
EcoRI GAATTC 1 cut(s) 1357
EcoRII CCWGG 2 cut(s) 53, 1009
EcoT14I CCWWGG 2 cut(s) 465, 675
EcoT22I ATGCAT 1 cut(s) 283
ErhI CCWWGG 2 cut(s) 465, 675
FaeI CATG 4 cut(s) 419, 733, 1042, 1334
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 3 cut(s) 58, 271, 657
FatI CATG 4 cut(s) 415, 729, 1038, 1330
FauNDI CATATG 1 cut(s) 142
Fnu4HI GCNGC 5 cut(s) 15, 102, 254, 734, 840
FokI GGATG 3 cut(s) 482, 920, 1001
Fsp4HI GCNGC 5 cut(s) 15, 102, 254, 734, 840
FspBI CTAG 4 cut(s) 203, 290, 1073, 1482
GlaI GCGC 3 cut(s) 164, 482, 1469
GluI GCNGC 5 cut(s) 15, 102, 254, 734, 840
GsaI CCCAGC 1 cut(s) 1436
GsuI CTGGAG 1 cut(s) 1296
HaeIII GGCC 7 cut(s) 488, 547, 680, 825, 1077, 1096, 1201
HhaI GCGC 3 cut(s) 165, 483, 1470
Hin1II CATG 4 cut(s) 419, 733, 1042, 1334
Hin6I GCGC 3 cut(s) 163, 481, 1468
HinP1I GCGC 3 cut(s) 163, 481, 1468
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 4 cut(s) 186, 263, 425, 434
HinfI GANTC 9 cut(s) 412, 662, 770, 796, 915, 1159, 1169, 1327, 1439
HphI GGTGA 6 cut(s) 400, 539, 805, 1130, 1330, 1346
Hpy166II GTNNAC 3 cut(s) 172, 692, 1231
Hpy188I TCNGA 3 cut(s) 1304, 1524, 1554
Hpy188III TCNNGA 7 cut(s) 36, 416, 567, 800, 988, 1163, 1212
Hpy8I GTNNAC 3 cut(s) 172, 692, 1231
HpyAV CCTTC 2 cut(s) 705, 1315
HpyCH4III ACNGT 4 cut(s) 379, 401, 1196, 1563
HpyCH4IV ACGT 1 cut(s) 372
HpyF10VI GCNNNNNNNGC 3 cut(s) 262, 433, 442
HpyF3I CTNAG 5 cut(s) 111, 261, 1282, 1301, 1551
HpySE526I ACGT 1 cut(s) 372
Hsp92II CATG 4 cut(s) 419, 733, 1042, 1334
HspAI GCGC 3 cut(s) 163, 481, 1468
Kzo9I GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
LmnI GCTCC 4 cut(s) 519, 715, 1315, 1540
Lsp1109I GCAGC 4 cut(s) 88, 240, 745, 826
LweI GCATC 4 cut(s) 1023, 1111, 1234, 1339
MaeI CTAG 4 cut(s) 203, 290, 1073, 1482
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 6 cut(s) 205, 373, 862, 892, 920, 1493
MalI GATC 6 cut(s) 86, 560, 858, 1086, 1263, 1411
MboI GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
MflI RGATCY 1 cut(s) 1084
MhlI GDGCHC 2 cut(s) 363, 1537
MluCI AATT 5 cut(s) 573, 761, 954, 1357, 1393
MlyI GAGTC 5 cut(s) 421, 779, 924, 1168, 1433
Mph1103I ATGCAT 1 cut(s) 283
MroXI GAANNNNTTC 4 cut(s) 631, 807, 1068, 1448
MseI TTAA 5 cut(s) 588, 776, 851, 960, 1509
MslI CAYNNNNRTG 2 cut(s) 141, 1463
MspR9I CCNGG 2 cut(s) 55, 1011
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 2 cut(s) 55, 1011
MwoI GCNNNNNNNGC 3 cut(s) 262, 433, 442
NdeI CATATG 1 cut(s) 142
NdeII GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
NlaIII CATG 4 cut(s) 419, 733, 1042, 1334
NlaIV GGNNCC 2 cut(s) 1179, 1311
NmuCI GTSAC 3 cut(s) 373, 862, 1493
NsiI ATGCAT 1 cut(s) 283
NspI RCATGY 1 cut(s) 733
PaeI GCATGC 1 cut(s) 733
PagI TCATGA 1 cut(s) 415
PceI AGGCCT 3 cut(s) 825, 1077, 1096
PctI GAATGC 1 cut(s) 1158
PdmI GAANNNNTTC 4 cut(s) 631, 807, 1068, 1448
PfeI GAWTC 4 cut(s) 662, 796, 1169, 1327
PkrI GCNGC 5 cut(s) 16, 103, 255, 735, 841
Ple19I CGATCG 1 cut(s) 561
PleI GAGTC 5 cut(s) 420, 778, 923, 1167, 1433
PpsI GAGTC 5 cut(s) 420, 778, 923, 1167, 1433
PsiI TTATAA 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 53, 1009
PspFI CCCAGC 1 cut(s) 1432
PspGI CCWGG 2 cut(s) 53, 1009
PspN4I GGNNCC 2 cut(s) 1179, 1311
PspPI GGNCC 2 cut(s) 545, 1200
PsuI RGATCY 1 cut(s) 1084
PvuI CGATCG 1 cut(s) 561
RsaI GTAC 4 cut(s) 270, 398, 1287, 1486
RsaNI GTAC 4 cut(s) 269, 397, 1286, 1485
RseI CAYNNNNRTG 2 cut(s) 141, 1463
SaqAI TTAA 5 cut(s) 588, 776, 851, 960, 1509
SatI GCNGC 5 cut(s) 15, 102, 254, 734, 840
Sau3AI GATC 6 cut(s) 84, 558, 856, 1084, 1261, 1409
Sau96I GGNCC 2 cut(s) 545, 1200
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 5 cut(s) 421, 779, 924, 1168, 1433
ScrFI CCNGG 2 cut(s) 55, 1011
SduI GDGCHC 2 cut(s) 363, 1537
SfaNI GCATC 4 cut(s) 1023, 1111, 1234, 1339
SmiMI CAYNNNNRTG 2 cut(s) 141, 1463
SpeI ACTAGT 1 cut(s) 1481
SphI GCATGC 1 cut(s) 733
Sse9I AATT 5 cut(s) 573, 761, 954, 1357, 1393
SseBI AGGCCT 3 cut(s) 825, 1077, 1096
SsiI CCGC 3 cut(s) 14, 1267, 1402
SspI AATATT 1 cut(s) 1069
SspMI CTAG 4 cut(s) 203, 290, 1073, 1482
StuI AGGCCT 3 cut(s) 825, 1077, 1096
StyD4I CCNGG 2 cut(s) 53, 1009
StyI CCWWGG 2 cut(s) 465, 675
TaaI ACNGT 4 cut(s) 379, 401, 1196, 1563
TaiI ACGT 1 cut(s) 375
TaqI TCGA 4 cut(s) 87, 557, 1172, 1442
TasI AATT 5 cut(s) 573, 761, 954, 1357, 1393
TatI WGTACW 2 cut(s) 396, 1484
TauI GCSGC 1 cut(s) 17
TfiI GAWTC 4 cut(s) 662, 796, 1169, 1327
Tru1I TTAA 5 cut(s) 588, 776, 851, 960, 1509
Tru9I TTAA 5 cut(s) 588, 776, 851, 960, 1509
TscAI CASTG 6 cut(s) 130, 496, 1111, 1320, 1411, 1498
TseFI GTSAC 3 cut(s) 373, 862, 1493
TseI GCWGC 4 cut(s) 101, 253, 733, 839
Tsp45I GTSAC 3 cut(s) 373, 862, 1493
TspRI CASTG 6 cut(s) 130, 496, 1111, 1320, 1411, 1498
XagI CCTNNNNNAGG 1 cut(s) 1524
XapI RAATTY 2 cut(s) 954, 1357
XceI RCATGY 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XmnI GAANNNNTTC 4 cut(s) 631, 807, 1068, 1448
XspI CTAG 4 cut(s) 203, 290, 1073, 1482
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.