MD17G1280500.v1.1

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009135
Physical Location & Seq
Forward (+)
34049627 .. 34056382
6756 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1280500.v1.1.491

Sequence Viewer

Length: 1593 bp
ATGGTTGTGCTTGCTGCTTCCATTATAAGCAAGTCTGGGAAAGTGCTTGTTTCTAGACAATTTGTGGATATGTCTCGTATAAGAATTGAGGGTCTTCTTGCGGCTTTCCCTAAGTTGGTAGGCACTGGCAAACAACATACTTATGTTGAGACAGAAAATGTCCGCTATGTTTACCAGCCTATAGAAGCTTTGTTCTTGGTACTTGTTACAAACAAGCAGAGCAATATATTGGAAGATTTGGAGACTCTGAGGCTACTCTCTAAACTTGCTCCCGAATACGCTATGTCTCTAGATGAAGAGGGTATATGCAGGACAGCTTTCGAGCTGATTTTTGCTTTTGATGAAGTCATCTCTCTTGGGCACAAGGAAAATGTGACTGTTGCACAGGTGAGGCAGTACTGTGAAATGGAGAGTCATGAAGAGAAACTGCACAAGCTTGTGATGCAGAGCAAGATAAATGAGACCAAGGATGTTATGAAACGGAAGGCTAGTGAGATTGACAAAAGCAAGATTGACAGGGGCAGAGCTGATAAAGGCGGATTTATGTCTTTACAGTCAATGGGATCTGGAAAAATTGATAGTGCATTTAGTGAGATGAGCATATCCAGTAGTGGAAGTGGCTTTGGAAGTGGGTCTGGTTTCGGATTGAGCACTGATATCGACTCATTTTCCACAAAGACTAAAGGTCGTCCACCTTCATCTGCTACTGCTCCTCCAAAGGGTTTGGGTATGCAGTTGGGAAAATCACAAAGGACCAACCAATTTTTAGAGTCCTTGAAAGCAGAGGGAGAGGTGATTCTTGAAGATGTACAGCCAAAAGCAGGCCAACCTAAATCAGCTGCCCCGCCTCTGACTGATCCTGTCACTTTGAATGTTGAAGAGAAACTTAACGTAACACTTAAACGTGATGGTGGGTTGAGCAACTTTGATGTTCAAGGGACATTATCTCTGCAAATTCTTAACCAGGAAGATGCATATATCCAAGTCCAGATTGAAACTGGTGGAAATCCTGGCATCCTTTTCAAAACACACCCTAACATGAACAAAGAGCTATTCTCCAATAATAACATTCTAGCCCTAAAGGATACTTCTAGGCCTTTTCCCACTGGTCAAACTGGTGATGCAGGAGGTGTTGGTCTTTTGAGATGGAGAATGCAGACTTCAGATGAGTCAATGGTGCCCCTGACAATCAACTGTTGGCCCACTGTTTCCGGGAATGAAACTTATGTCAACATTGAGTATGAGGCTTCAGAGATGTTTGATCTGAGAAATGTTGTTATCTCTGTACCTCTCCCAGCTGCGAGAGAGGCTCCACGTGTAAACCAGATTGAAGGTGAATGGAGATACGATGCTAGGAATTCAATGTTGGAGTGGTCCATACTTCTCGTAGATAACTCGAACCGCAGTGGATCAATGGAGTTTGTTGTTCCCCCAGCAGACCCATCTGTATTTTTCCCCATTTCTGTGCGCTTTGCTGCCACTGAGACATTCAGTGACCTGAAGGTTTTGAACATTATACCTCTGCAAGGTGGAGCCGCTCCCAAGTTTTCTCAAAGAACGCAGTTGGTGACGGAGAATTACCAAGTGGTGTGA

Protein Analysis

531

Amino Acids

58.29

Weight (kDa)

5.4

Isoelectric Point (pI)

46.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adap_comp_sub PF00928 285 - 529 2.7e-42 Adaptor complexes medium subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 1177
AccBSI CCGCTC 1 cut(s) 1538
AciI CCGC 6 cut(s) 101, 163, 537, 845, 1402, 1536
AclWI GGATC 3 cut(s) 571, 851, 1417
AcsI RAATTY 2 cut(s) 954, 1357
AcuI CTGAAG 3 cut(s) 1146, 1233, 1520
AcvI CACGTG 1 cut(s) 1316
AfaI GTAC 4 cut(s) 201, 398, 810, 1287
AfiI CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
AflIII ACRYGT 1 cut(s) 1315
AhdI GACNNNNNGTC 1 cut(s) 684
AjnI CCWGG 2 cut(s) 963, 1009
AjuI GAANNNNNNNTTGG 2 cut(s) 1349, 1381
AluBI AGCT 8 cut(s) 188, 317, 325, 436, 527, 839, 1051, 1298
AluI AGCT 8 cut(s) 188, 317, 325, 436, 527, 839, 1051, 1298
Alw21I GWGCWC 1 cut(s) 653
Alw26I GTCTC 6 cut(s) 78, 143, 236, 291, 455, 1478
AlwI GGATC 3 cut(s) 571, 851, 1417
AoxI GGCC 3 cut(s) 823, 1094, 1199
ApeKI GCWGC 4 cut(s) 14, 839, 1298, 1475
ApoI RAATTY 2 cut(s) 954, 1357
AspLEI GCGC 1 cut(s) 1470
AspS9I GGNCC 3 cut(s) 753, 1200, 1374
AsuC2I CCSGG 1 cut(s) 1213
AsuHPI GGTGA 5 cut(s) 400, 805, 1130, 1346, 1579
AvaII GGWCC 2 cut(s) 753, 1374
BaeGI GKGCMC 2 cut(s) 363, 1182
BanI GGYRCC 1 cut(s) 1177
BbrPI CACGTG 1 cut(s) 1316
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 653
BbvI GCAGC 3 cut(s) 826, 1285, 1462
BccI CCATC 3 cut(s) 902, 1140, 1450
BcgI CGANNNNNNTGC 2 cut(s) 640, 674
BciT130I CCWGG 2 cut(s) 965, 1011
BciVI GTATCC 1 cut(s) 1078
BcnI CCSGG 1 cut(s) 1213
BcoDI GTCTC 6 cut(s) 78, 143, 236, 291, 455, 1478
BfaI CTAG 6 cut(s) 54, 290, 489, 1073, 1092, 1353
BfmI CTRYAG 1 cut(s) 180
BfuI GTATCC 1 cut(s) 1078
BisI GCNGC 6 cut(s) 15, 102, 840, 1299, 1476, 1536
BlsI GCNGC 6 cut(s) 16, 103, 841, 1300, 1477, 1537
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 3 cut(s) 965, 1011, 1213
Bme18I GGWCC 2 cut(s) 753, 1374
BmeRI GACNNNNNGTC 1 cut(s) 684
BmgT120I GGNCC 3 cut(s) 753, 1200, 1374
BmiI GGNNCC 3 cut(s) 1179, 1311, 1534
BmrFI CCNGG 3 cut(s) 965, 1011, 1213
BmsI GCATC 5 cut(s) 432, 961, 1023, 1111, 1339
BpiI GAAGAC 1 cut(s) 86
BplI GAGNNNNNCTC 4 cut(s) 1040, 1072, 1294, 1326
BpuMI CCSGG 1 cut(s) 1213
BsaAI YACGTR 1 cut(s) 1316
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 1 cut(s) 465
BsaXI ACNNNNNCTCC 2 cut(s) 697, 727
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
Bse1I ACTGG 5 cut(s) 130, 606, 1003, 1111, 1120
BseBI CCWGG 2 cut(s) 965, 1011
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 2 cut(s) 475, 1014
BseLI CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
BseMII CTCAG 3 cut(s) 239, 1256, 1473
BseNI ACTGG 5 cut(s) 130, 606, 1003, 1111, 1120
BseRI GAGGAG 1 cut(s) 702
BseSI GKGCMC 2 cut(s) 363, 1182
BseXI GCAGC 3 cut(s) 826, 1285, 1462
BseYI CCCAGC 2 cut(s) 1294, 1432
BsgI GTGCAG 1 cut(s) 413
BshFI GGCC 3 cut(s) 825, 1096, 1201
BshNI GGYRCC 1 cut(s) 1177
BsiHKAI GWGCWC 1 cut(s) 653
BsiSI CCGG 1 cut(s) 1212
BslFI GGGAC 1 cut(s) 952
BslI CCNNNNNNNGG 4 cut(s) 115, 719, 821, 1526
BsmAI GTCTC 6 cut(s) 78, 143, 236, 291, 455, 1478
BsmFI GGGAC 1 cut(s) 952
BsmI GAATGC 1 cut(s) 1158
BsnI GGCC 3 cut(s) 825, 1096, 1201
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 3 cut(s) 363, 653, 1182
Bsp1407I TGTACA 1 cut(s) 808
Bsp143I GATC 4 cut(s) 563, 856, 1261, 1409
BspACI CCGC 6 cut(s) 101, 163, 537, 845, 1402, 1536
BspANI GGCC 3 cut(s) 825, 1096, 1201
BspCNI CTCAG 3 cut(s) 240, 1257, 1474
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 1179, 1311, 1534
BspPI GGATC 3 cut(s) 571, 851, 1417
BspT107I GGYRCC 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 455
BsrBI CCGCTC 1 cut(s) 1538
BsrGI TGTACA 1 cut(s) 808
BsrI ACTGG 5 cut(s) 130, 606, 1003, 1111, 1120
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 4 cut(s) 563, 856, 1261, 1409
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 965, 1011
Bst4CI ACNGT 5 cut(s) 379, 401, 555, 1196, 1207
Bst6I CTCTTC 3 cut(s) 291, 414, 873
BstAUI TGTACA 1 cut(s) 808
BstBAI YACGTR 1 cut(s) 1316
BstC8I GCNNGC 2 cut(s) 12, 823
BstDEI CTNAG 4 cut(s) 111, 248, 1265, 1482
BstENI CCTNNNNNAGG 1 cut(s) 1524
BstF5I GGATG 2 cut(s) 475, 1014
BstHHI GCGC 1 cut(s) 1470
BstKTI GATC 4 cut(s) 566, 859, 1264, 1412
BstMAI GTCTC 6 cut(s) 78, 143, 236, 291, 455, 1478
BstMBI GATC 4 cut(s) 563, 856, 1261, 1409
BstMWI GCNNNNNNNGC 2 cut(s) 442, 1307
BstNI CCWGG 2 cut(s) 965, 1011
BstSCI CCNGG 3 cut(s) 963, 1009, 1211
BstSFI CTRYAG 1 cut(s) 180
BstSLI GKGCMC 2 cut(s) 363, 1182
BstV1I GCAGC 3 cut(s) 826, 1285, 1462
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 563
BstYI RGATCY 1 cut(s) 563
BsuI GTATCC 1 cut(s) 1078
BsuRI GGCC 3 cut(s) 825, 1096, 1201
BtsCI GGATG 2 cut(s) 475, 1014
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 7 cut(s) 123, 651, 1104, 1203, 1411, 1479, 1498
Cac8I GCNNGC 2 cut(s) 12, 823
CciI TCATGA 1 cut(s) 415
CfoI GCGC 1 cut(s) 1470
Cfr13I GGNCC 3 cut(s) 753, 1200, 1374
Csp6I GTAC 4 cut(s) 200, 397, 809, 1286
CviAII CATG 2 cut(s) 416, 1039
CviQI GTAC 4 cut(s) 200, 397, 809, 1286
DdeI CTNAG 4 cut(s) 111, 248, 1265, 1482
DpnI GATC 4 cut(s) 565, 858, 1263, 1411
DpnII GATC 4 cut(s) 563, 856, 1261, 1409
DriI GACNNNNNGTC 1 cut(s) 684
Eam1104I CTCTTC 3 cut(s) 291, 414, 873
Eam1105I GACNNNNNGTC 1 cut(s) 684
EarI CTCTTC 3 cut(s) 291, 414, 873
EciI GGCGGA 1 cut(s) 552
Eco130I CCWWGG 1 cut(s) 465
Eco147I AGGCCT 1 cut(s) 1096
Eco31I GGTCTC 1 cut(s) 455
Eco32I GATATC 1 cut(s) 658
Eco47I GGWCC 2 cut(s) 753, 1374
Eco57I CTGAAG 3 cut(s) 1146, 1233, 1520
Eco72I CACGTG 1 cut(s) 1316
EcoNI CCTNNNNNAGG 1 cut(s) 1524
EcoRI GAATTC 1 cut(s) 1357
EcoRII CCWGG 2 cut(s) 963, 1009
EcoRV GATATC 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 976
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 419, 1042
FalI AAGNNNNNCTT 2 cut(s) 870, 902
FaqI GGGAC 1 cut(s) 952
FatI CATG 2 cut(s) 415, 1038
FauI CCCGC 1 cut(s) 852
Fnu4HI GCNGC 6 cut(s) 15, 102, 840, 1299, 1476, 1536
FokI GGATG 2 cut(s) 482, 1001
Fsp4HI GCNGC 6 cut(s) 15, 102, 840, 1299, 1476, 1536
FspBI CTAG 6 cut(s) 54, 290, 489, 1073, 1092, 1353
GlaI GCGC 1 cut(s) 1469
GluI GCNGC 6 cut(s) 15, 102, 840, 1299, 1476, 1536
GsaI CCCAGC 2 cut(s) 1298, 1436
HaeIII GGCC 3 cut(s) 825, 1096, 1201
HapII CCGG 1 cut(s) 1212
HhaI GCGC 1 cut(s) 1470
Hin1II CATG 2 cut(s) 419, 1042
Hin6I GCGC 1 cut(s) 1468
HinP1I GCGC 1 cut(s) 1468
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 2 cut(s) 186, 434
HinfI GANTC 6 cut(s) 244, 412, 662, 770, 796, 1169
HpaII CCGG 1 cut(s) 1212
HphI GGTGA 5 cut(s) 400, 805, 1130, 1346, 1579
Hpy166II GTNNAC 4 cut(s) 172, 692, 1231, 1321
Hpy188I TCNGA 6 cut(s) 249, 644, 852, 1165, 1252, 1266
Hpy188III TCNNGA 7 cut(s) 54, 272, 290, 416, 567, 800, 988
Hpy8I GTNNAC 4 cut(s) 172, 692, 1231, 1321
HpyAV CCTTC 4 cut(s) 478, 705, 1325, 1495
HpyCH4III ACNGT 5 cut(s) 379, 401, 555, 1196, 1207
HpyCH4IV ACGT 3 cut(s) 891, 904, 1315
HpyF10VI GCNNNNNNNGC 2 cut(s) 442, 1307
HpyF3I CTNAG 4 cut(s) 111, 248, 1265, 1482
HpySE526I ACGT 3 cut(s) 891, 904, 1315
Hsp92II CATG 2 cut(s) 419, 1042
HspAI GCGC 1 cut(s) 1468
Kzo9I GATC 4 cut(s) 563, 856, 1261, 1409
LmnI GCTCC 5 cut(s) 274, 715, 1315, 1532, 1543
Lsp1109I GCAGC 3 cut(s) 826, 1285, 1462
LweI GCATC 5 cut(s) 432, 961, 1023, 1111, 1339
MaeI CTAG 6 cut(s) 54, 290, 489, 1073, 1092, 1353
MaeII ACGT 3 cut(s) 891, 904, 1315
MaeIII GTNAC 6 cut(s) 205, 373, 862, 892, 1493, 1567
MalI GATC 4 cut(s) 565, 858, 1263, 1411
MbiI CCGCTC 1 cut(s) 1538
MboI GATC 4 cut(s) 563, 856, 1261, 1409
MboII GAAGA 7 cut(s) 86, 245, 308, 431, 815, 890, 980
MflI RGATCY 1 cut(s) 563
MhlI GDGCHC 3 cut(s) 363, 653, 1182
MluCI AATT 7 cut(s) 59, 84, 573, 761, 954, 1357, 1576
MlyI GAGTC 5 cut(s) 238, 421, 656, 779, 1178
MmeI TCCRAC 1 cut(s) 1347
Mph1103I ATGCAT 1 cut(s) 976
MseI TTAA 3 cut(s) 888, 900, 960
MslI CAYNNNNRTG 2 cut(s) 141, 1463
MspA1I CMGCKG 2 cut(s) 839, 1298
MspI CCGG 1 cut(s) 1212
MspR9I CCNGG 3 cut(s) 965, 1011, 1213
Mva1269I GAATGC 1 cut(s) 1158
MvaI CCWGG 2 cut(s) 965, 1011
MwoI GCNNNNNNNGC 2 cut(s) 442, 1307
NciI CCSGG 1 cut(s) 1213
NdeII GATC 4 cut(s) 563, 856, 1261, 1409
NlaIII CATG 2 cut(s) 419, 1042
NlaIV GGNNCC 3 cut(s) 1179, 1311, 1534
NmuCI GTSAC 4 cut(s) 373, 862, 1493, 1567
NsiI ATGCAT 1 cut(s) 976
PagI TCATGA 1 cut(s) 415
PceI AGGCCT 1 cut(s) 1096
PctI GAATGC 1 cut(s) 1158
PfeI GAWTC 1 cut(s) 796
PfoI TCCNGGA 1 cut(s) 1211
PkrI GCNGC 6 cut(s) 16, 103, 841, 1300, 1477, 1537
PleI GAGTC 5 cut(s) 238, 420, 656, 778, 1177
PmaCI CACGTG 1 cut(s) 1316
PmlI CACGTG 1 cut(s) 1316
PpsI GAGTC 5 cut(s) 238, 420, 656, 778, 1177
Ppu21I YACGTR 1 cut(s) 1316
PsiI TTATAA 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 963, 1009
PspCI CACGTG 1 cut(s) 1316
PspFI CCCAGC 2 cut(s) 1294, 1432
PspGI CCWGG 2 cut(s) 963, 1009
PspN4I GGNNCC 3 cut(s) 1179, 1311, 1534
PspPI GGNCC 3 cut(s) 753, 1200, 1374
PsuI RGATCY 1 cut(s) 563
PvuII CAGCTG 2 cut(s) 839, 1298
RsaI GTAC 4 cut(s) 201, 398, 810, 1287
RsaNI GTAC 4 cut(s) 200, 397, 809, 1286
RseI CAYNNNNRTG 2 cut(s) 141, 1463
SaqAI TTAA 3 cut(s) 888, 900, 960
SatI GCNGC 6 cut(s) 15, 102, 840, 1299, 1476, 1536
Sau3AI GATC 4 cut(s) 563, 856, 1261, 1409
Sau96I GGNCC 3 cut(s) 753, 1200, 1374
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 5 cut(s) 238, 421, 656, 779, 1178
ScrFI CCNGG 3 cut(s) 965, 1011, 1213
SduI GDGCHC 3 cut(s) 363, 653, 1182
SfaNI GCATC 5 cut(s) 432, 961, 1023, 1111, 1339
SfcI CTRYAG 1 cut(s) 180
SinI GGWCC 2 cut(s) 753, 1374
SmiMI CAYNNNNRTG 2 cut(s) 141, 1463
Sse9I AATT 7 cut(s) 59, 84, 573, 761, 954, 1357, 1576
SseBI AGGCCT 1 cut(s) 1096
SsiI CCGC 6 cut(s) 101, 163, 537, 845, 1402, 1536
SspMI CTAG 6 cut(s) 54, 290, 489, 1073, 1092, 1353
StuI AGGCCT 1 cut(s) 1096
StyD4I CCNGG 3 cut(s) 963, 1009, 1211
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 5 cut(s) 379, 401, 555, 1196, 1207
TaiI ACGT 3 cut(s) 894, 907, 1318
TaqI TCGA 3 cut(s) 321, 660, 1397
TasI AATT 7 cut(s) 59, 84, 573, 761, 954, 1357, 1576
TatI WGTACW 2 cut(s) 396, 808
TauI GCSGC 2 cut(s) 104, 1538
TfiI GAWTC 1 cut(s) 796
Tru1I TTAA 3 cut(s) 888, 900, 960
Tru9I TTAA 3 cut(s) 888, 900, 960
TscAI CASTG 7 cut(s) 130, 658, 1111, 1210, 1411, 1486, 1498
TseFI GTSAC 4 cut(s) 373, 862, 1493, 1567
TseI GCWGC 4 cut(s) 14, 839, 1298, 1475
Tsp45I GTSAC 4 cut(s) 373, 862, 1493, 1567
TspDTI ATGAA 7 cut(s) 309, 357, 432, 491, 687, 1055, 1233
TspGWI ACGGA 2 cut(s) 496, 1586
TspRI CASTG 7 cut(s) 130, 658, 1111, 1210, 1411, 1486, 1498
VpaK11BI GGWCC 2 cut(s) 753, 1374
XagI CCTNNNNNAGG 1 cut(s) 1524
XapI RAATTY 2 cut(s) 954, 1357
XbaI TCTAGA 2 cut(s) 53, 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 995
XspI CTAG 6 cut(s) 54, 290, 489, 1073, 1092, 1353
ZrmI AGTACT 1 cut(s) 398
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.