Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0007828.1

Orthogroup ID

OG0009135

Physical Location & Seq

Genomic Coordinates Forward (+)

17094 .. 22538

Gene Structure 5445 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0007828.1_g000007.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1593 bp

atggttgttcttgcggcttccgttataaacaagtctgggaaagtgcttgtttcgaggcagtttatggatatgtctcgtataaggattgagggtcttcttgcggctttccctaagttggtaggcactggcaaacaacatacatatgtcgagacggaaaatgtgcgctatgtttaccagccaatagaagctttgtatttgcttctagttacaaacaagcagagcaatatcttagaagatttggagactttgaggctgctctctaagcttgtacctgaatatgcattgtccctagatgaagagggtatatgcaggacagctttcgagttgatttttgcttttgatgaagtaatctctcttgggcacaaggagaatgtgactgttgcacaggtgaagcagtactgtgagatggagagtcatgaagagaagcttcacaagctcgtaatgcagagcaagataaatgagaccaaggatgttatgaagcgtaaagccagtgagattgacaaaagcaagattgataggagcagaggtgaaaagggaggatttgggcctttgcagtcaatgggttctggaagaattgagagtgcctttagtgagatgagcttatccagcactggaagtggttttggaagtggttctggtttgggtgcgaccactgattttgaaacattttcttcgaaggccaaaggccgtccaccttcatctgccacagctcccccaaagggtttaggcatgcagcttggaaaatcacagaggaccaaccaatttttagagtccttaaaagcggagggagaggtgattcttgaagatgtacagccaaaagcaggccagtcaaaatcagttgccccacctttaactgatcccgtcactttgaatgtcgaagagaaacttaatgtaacacttaaacgtgatggtggagtcagtaactttgatgttcaaggaacattatccctgcaaattcttaaccaagaagacgggcatatccaagtccagattgaaaccggtggaaatcctggcatcctttttaaaactcaccctaacatgaacaaagaactattttctaatgaaaatattctaggcttgaaagatcctaataggcctttccccactggtcaagctggtgatgcagcaggtgttggtcttttgaaatggagaatgcagagtcaagatgaatcattggtgcctctacaaatcaactgttggccctctgtttctggaaatgaaacctatgtcaacattgagtatgatgcttcatcaatgttcgatctgcgaaatgttgtcatttcagtacctcttccagctctcagagaggctccagttgtgaaggagattcatggtgaatggagatatgatgcaaggaattcagtattggagtggtctatacttcttattgataactcaaaccgcagtggatcaatggagtttgttgttcctccagcagactcgaatgtattctttcccatttctgtgcgcttctctgccactagtacattcagtgacctgaaggttgtgaatatcttacctctgaaaggtggagcccctccgaagttcgctcagagaacacagttgcttacagagaactaccaggtggtgtaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005798 GO:0005829 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0055044 GO:0097708

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Adap_comp_sub

Protein Analysis

530

Amino Acids

58.23

Weight (kDa)

5.72

Isoelectric Point (pI)

50.71

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05010 AT5G05010
fragaria_vesca	FvH4_5g25700 FvH4_5g25700 FvH4_5g25700 FvH4_5g27071 FvH4_5g27071
malus_domestica	MD09G1288200.v1.1 MD17G1280500.v1.1
prunus_persica	Prupe.3G005000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0216031
rosa_laevigata	RLG00000002598
rosa_multiflora	Rmu_sc0002356.1_g000010 Rmu_sc0007828.1_g000007
rosa_roxburghii	Rroxscaffold_3G00243720
rosa_rugosa	Rorug07G0158300 Rorug07G0158300
rosa_samantha	Rh7AG295500 Rh7BG287600 Rh7CG315900 Rh7DG298400
rosa_wichuraiana	Rw7G025130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	26
AarI	CACCTGC	1 cut(s)	1118
Acc36I	ACCTGC	1 cut(s)	1118
AccB1I	GGYRCC	1 cut(s)	1177
AciI	CCGC	4 cut(s)	14, 101, 782, 1402
AclWI	GGATC	3 cut(s)	851, 1079, 1417
AcsI	RAATTY	2 cut(s)	954, 1357
AcuI	CTGAAG	1 cut(s)	1520
AfaI	GTAC	5 cut(s)	270, 398, 810, 1287, 1486
AfiI	CCNNNNNNNGG	4 cut(s)	115, 719, 821, 1526
AgeI	ACCGGT	1 cut(s)	998
AgsI	TTSAA	7 cut(s)	662, 803, 871, 935, 995, 1081, 1144
AhlI	ACTAGT	1 cut(s)	1481
AjnI	CCWGG	2 cut(s)	1009, 1581
AjuI	GAANNNNNNNTTGG	2 cut(s)	1349, 1381
Alw26I	GTCTC	4 cut(s)	78, 143, 236, 455
AlwI	GGATC	3 cut(s)	851, 1079, 1417
AoxI	GGCC	6 cut(s)	545, 678, 685, 823, 1094, 1199
ApeKI	GCWGC	3 cut(s)	253, 733, 1124
ApoI	RAATTY	2 cut(s)	954, 1357
AsiGI	ACCGGT	1 cut(s)	998
Asp700I	GAANNNNTTC	3 cut(s)	631, 1068, 1448
AspLEI	GCGC	2 cut(s)	165, 1470
AspS9I	GGNCC	3 cut(s)	545, 753, 1200
AsuHPI	GGTGA	6 cut(s)	400, 539, 805, 1022, 1130, 1346
AsuII	TTCGAA	1 cut(s)	674
AvaII	GGWCC	1 cut(s)	753
BaeGI	GKGCMC	1 cut(s)	363
BanI	GGYRCC	1 cut(s)	1177
BanII	GRGCYC	1 cut(s)	1537
BbsI	GAAGAC	2 cut(s)	86, 975
BbvI	GCAGC	3 cut(s)	240, 745, 1136
BccI	CCATC	2 cut(s)	400, 902
BceAI	ACGGC	1 cut(s)	672
BciT130I	CCWGG	2 cut(s)	1011, 1583
BcoDI	GTCTC	4 cut(s)	78, 143, 236, 455
BcuI	ACTAGT	1 cut(s)	1481
BfaI	CTAG	4 cut(s)	203, 290, 1073, 1482
BfuAI	ACCTGC	1 cut(s)	1118
BisI	GCNGC	5 cut(s)	15, 102, 254, 734, 1125
BlsI	GCNGC	5 cut(s)	16, 103, 255, 735, 1126
BmcAI	AGTACT	1 cut(s)	398
Bme1390I	CCNGG	2 cut(s)	1011, 1583
Bme18I	GGWCC	1 cut(s)	753
BmgT120I	GGNCC	3 cut(s)	545, 753, 1200
BmiI	GGNNCC	3 cut(s)	1179, 1311, 1534
BmrFI	CCNGG	2 cut(s)	1011, 1583
BmsI	GCATC	4 cut(s)	1023, 1111, 1234, 1339
BpiI	GAAGAC	2 cut(s)	86, 975
BpmI	CTGGAG	2 cut(s)	1296, 1416
Bpu14I	TTCGAA	1 cut(s)	674
BsaI	GGTCTC	1 cut(s)	455
BsaJI	CCNNGG	1 cut(s)	465
BsaWI	WCCGGW	1 cut(s)	998
BsaXI	ACNNNNNCTCC	2 cut(s)	511, 541
Bsc4I	CCNNNNNNNGG	4 cut(s)	115, 719, 821, 1526
Bse118I	RCCGGY	1 cut(s)	998
Bse1I	ACTGG	6 cut(s)	130, 489, 616, 826, 1111, 1313
BseBI	CCWGG	2 cut(s)	1011, 1583
BseDI	CCNNGG	1 cut(s)	465
BseGI	GGATG	2 cut(s)	475, 1014
BseLI	CCNNNNNNNGG	4 cut(s)	115, 719, 821, 1526
BseMII	CTCAG	2 cut(s)	1315, 1565
BseNI	ACTGG	6 cut(s)	130, 489, 616, 826, 1111, 1313
BseSI	GKGCMC	1 cut(s)	363
BseXI	GCAGC	3 cut(s)	240, 745, 1136
BshFI	GGCC	6 cut(s)	547, 680, 687, 825, 1096, 1201
BshNI	GGYRCC	1 cut(s)	1177
BshTI	ACCGGT	1 cut(s)	998
BsiSI	CCGG	1 cut(s)	999
BslFI	GGGAC	1 cut(s)	271
BslI	CCNNNNNNNGG	4 cut(s)	115, 719, 821, 1526
BsmAI	GTCTC	4 cut(s)	78, 143, 236, 455
BsmBI	CGTCTC	1 cut(s)	143
BsmFI	GGGAC	1 cut(s)	271
BsmI	GAATGC	1 cut(s)	1158
BsnI	GGCC	6 cut(s)	547, 680, 687, 825, 1096, 1201
Bso31I	GGTCTC	1 cut(s)	455
Bsp119I	TTCGAA	1 cut(s)	674
Bsp1286I	GDGCHC	2 cut(s)	363, 1537
Bsp1407I	TGTACA	1 cut(s)	808
Bsp143I	GATC	4 cut(s)	856, 1084, 1261, 1409
BspACI	CCGC	4 cut(s)	14, 101, 782, 1402
BspANI	GGCC	6 cut(s)	547, 680, 687, 825, 1096, 1201
BspCNI	CTCAG	2 cut(s)	1314, 1564
BspHI	TCATGA	1 cut(s)	415
BspLI	GGNNCC	3 cut(s)	1179, 1311, 1534
BspMI	ACCTGC	1 cut(s)	1118
BspPI	GGATC	3 cut(s)	851, 1079, 1417
BspT104I	TTCGAA	1 cut(s)	674
BspT107I	GGYRCC	1 cut(s)	1177
BspTNI	GGTCTC	1 cut(s)	455
BsrFI	RCCGGY	1 cut(s)	998
BsrGI	TGTACA	1 cut(s)	808
BsrI	ACTGG	6 cut(s)	130, 489, 616, 826, 1111, 1313
BssAI	RCCGGY	1 cut(s)	998
BssECI	CCNNGG	1 cut(s)	465
BssMI	GATC	4 cut(s)	856, 1084, 1261, 1409
BssT1I	CCWWGG	1 cut(s)	465
Bst2UI	CCWGG	2 cut(s)	1011, 1583
Bst4CI	ACNGT	4 cut(s)	379, 401, 1196, 1563
Bst6I	CTCTTC	4 cut(s)	291, 414, 873, 1296
BstAUI	TGTACA	1 cut(s)	808
BstBI	TTCGAA	1 cut(s)	674
BstC8I	GCNNGC	2 cut(s)	731, 823
BstDEI	CTNAG	5 cut(s)	111, 229, 261, 1301, 1551
BstENI	CCTNNNNNAGG	1 cut(s)	1524
BstF5I	GGATG	2 cut(s)	475, 1014
BstHHI	GCGC	2 cut(s)	165, 1470
BstKTI	GATC	4 cut(s)	859, 1087, 1264, 1412
BstMAI	GTCTC	4 cut(s)	78, 143, 236, 455
BstMBI	GATC	4 cut(s)	856, 1084, 1261, 1409
BstMWI	GCNNNNNNNGC	5 cut(s)	262, 433, 442, 606, 1121
BstNI	CCWGG	2 cut(s)	1011, 1583
BstNSI	RCATGY	1 cut(s)	733
BstSCI	CCNGG	2 cut(s)	1009, 1581
BstSLI	GKGCMC	1 cut(s)	363
BstV1I	GCAGC	3 cut(s)	240, 745, 1136
BstV2I	GAAGAC	2 cut(s)	86, 975
BstX2I	RGATCY	1 cut(s)	1084
BstYI	RGATCY	1 cut(s)	1084
BsuRI	GGCC	6 cut(s)	547, 680, 687, 825, 1096, 1201
BtsCI	GGATG	2 cut(s)	475, 1014
BtsI	GCAGTG	1 cut(s)	1411
BtsIMutI	CAGTG	7 cut(s)	123, 496, 609, 651, 1104, 1411, 1498
BveI	ACCTGC	1 cut(s)	1118
Cac8I	GCNNGC	2 cut(s)	731, 823
CciI	TCATGA	1 cut(s)	415
CfoI	GCGC	2 cut(s)	165, 1470
Cfr10I	RCCGGY	1 cut(s)	998
Cfr13I	GGNCC	3 cut(s)	545, 753, 1200
CsiI	ACCWGGT	1 cut(s)	1581
Csp6I	GTAC	5 cut(s)	269, 397, 809, 1286, 1485
CspAI	ACCGGT	1 cut(s)	998
CspCI	CAANNNNNGTGG	2 cut(s)	640, 675
CviAII	CATG	4 cut(s)	416, 730, 1039, 1331
CviQI	GTAC	5 cut(s)	269, 397, 809, 1286, 1485
DdeI	CTNAG	5 cut(s)	111, 229, 261, 1301, 1551
DpnI	GATC	4 cut(s)	858, 1086, 1263, 1411
DpnII	GATC	4 cut(s)	856, 1084, 1261, 1409
DraI	TTTAAA	1 cut(s)	1024
Eam1104I	CTCTTC	4 cut(s)	291, 414, 873, 1296
EarI	CTCTTC	4 cut(s)	291, 414, 873, 1296
Eco130I	CCWWGG	1 cut(s)	465
Eco147I	AGGCCT	1 cut(s)	1096
Eco24I	GRGCYC	1 cut(s)	1537
Eco31I	GGTCTC	1 cut(s)	455
Eco47I	GGWCC	1 cut(s)	753
Eco57I	CTGAAG	1 cut(s)	1520
EcoNI	CCTNNNNNAGG	1 cut(s)	1524
EcoRI	GAATTC	1 cut(s)	1357
EcoRII	CCWGG	2 cut(s)	1009, 1581
EcoT14I	CCWWGG	1 cut(s)	465
EcoT22I	ATGCAT	1 cut(s)	283
EcoT38I	GRGCYC	1 cut(s)	1537
ErhI	CCWWGG	1 cut(s)	465
Esp3I	CGTCTC	1 cut(s)	143
FaeI	CATG	4 cut(s)	419, 733, 1042, 1334
FalI	AAGNNNNNCTT	4 cut(s)	411, 443, 870, 902
FaqI	GGGAC	1 cut(s)	271
FatI	CATG	4 cut(s)	415, 729, 1038, 1330
FauNDI	CATATG	1 cut(s)	142
Fnu4HI	GCNGC	5 cut(s)	15, 102, 254, 734, 1125
FokI	GGATG	2 cut(s)	482, 1001
FriOI	GRGCYC	1 cut(s)	1537
Fsp4HI	GCNGC	5 cut(s)	15, 102, 254, 734, 1125
FspBI	CTAG	4 cut(s)	203, 290, 1073, 1482
GlaI	GCGC	2 cut(s)	164, 1469
GluI	GCNGC	5 cut(s)	15, 102, 254, 734, 1125
GsuI	CTGGAG	2 cut(s)	1296, 1416
HaeIII	GGCC	6 cut(s)	547, 680, 687, 825, 1096, 1201
HapII	CCGG	1 cut(s)	999
HhaI	GCGC	2 cut(s)	165, 1470
Hin1II	CATG	4 cut(s)	419, 733, 1042, 1334
Hin6I	GCGC	2 cut(s)	163, 1468
HinP1I	GCGC	2 cut(s)	163, 1468
HincII	GTYRAC	1 cut(s)	1231
HindII	GTYRAC	1 cut(s)	1231
HindIII	AAGCTT	3 cut(s)	186, 263, 425
HinfI	GANTC	8 cut(s)	412, 770, 796, 915, 1159, 1169, 1327, 1439
HpaII	CCGG	1 cut(s)	999
HphI	GGTGA	6 cut(s)	400, 539, 805, 1022, 1130, 1346
Hpy166II	GTNNAC	3 cut(s)	172, 692, 1231
Hpy188I	TCNGA	4 cut(s)	1304, 1524, 1542, 1554
Hpy188III	TCNNGA	7 cut(s)	148, 416, 567, 800, 988, 1163, 1212
Hpy8I	GTNNAC	3 cut(s)	172, 692, 1231
HpyAV	CCTTC	4 cut(s)	670, 705, 1315, 1495
HpyCH4III	ACNGT	4 cut(s)	379, 401, 1196, 1563
HpyCH4IV	ACGT	1 cut(s)	904
HpyF10VI	GCNNNNNNNGC	5 cut(s)	262, 433, 442, 606, 1121
HpyF3I	CTNAG	5 cut(s)	111, 229, 261, 1301, 1551
HpySE526I	ACGT	1 cut(s)	904
Hsp92II	CATG	4 cut(s)	419, 733, 1042, 1334
HspAI	GCGC	2 cut(s)	163, 1468
Kzo9I	GATC	4 cut(s)	856, 1084, 1261, 1409
LmnI	GCTCC	4 cut(s)	519, 715, 1315, 1532
Lsp1109I	GCAGC	3 cut(s)	240, 745, 1136
LweI	GCATC	4 cut(s)	1023, 1111, 1234, 1339
MabI	ACCWGGT	1 cut(s)	1581
MaeI	CTAG	4 cut(s)	203, 290, 1073, 1482
MaeII	ACGT	1 cut(s)	904
MaeIII	GTNAC	6 cut(s)	205, 373, 862, 892, 920, 1493
MalI	GATC	4 cut(s)	858, 1086, 1263, 1411
MboI	GATC	4 cut(s)	856, 1084, 1261, 1409
MflI	RGATCY	1 cut(s)	1084
MhlI	GDGCHC	2 cut(s)	363, 1537
MluCI	AATT	4 cut(s)	573, 761, 954, 1357
MlyI	GAGTC	5 cut(s)	421, 779, 924, 1168, 1433
Mph1103I	ATGCAT	1 cut(s)	283
MroXI	GAANNNNTTC	3 cut(s)	631, 1068, 1448
MseI	TTAA	6 cut(s)	776, 851, 888, 900, 960, 1023
MslI	CAYNNNNRTG	2 cut(s)	141, 1463
MspI	CCGG	1 cut(s)	999
MspR9I	CCNGG	2 cut(s)	1011, 1583
Mva1269I	GAATGC	1 cut(s)	1158
MvaI	CCWGG	2 cut(s)	1011, 1583
MwoI	GCNNNNNNNGC	5 cut(s)	262, 433, 442, 606, 1121
NdeI	CATATG	1 cut(s)	142
NdeII	GATC	4 cut(s)	856, 1084, 1261, 1409
NlaIII	CATG	4 cut(s)	419, 733, 1042, 1334
NlaIV	GGNNCC	3 cut(s)	1179, 1311, 1534
NmuCI	GTSAC	3 cut(s)	373, 862, 1493
NsiI	ATGCAT	1 cut(s)	283
NspI	RCATGY	1 cut(s)	733
NspV	TTCGAA	1 cut(s)	674
PaeI	GCATGC	1 cut(s)	733
PagI	TCATGA	1 cut(s)	415
PaqCI	CACCTGC	1 cut(s)	1118
PceI	AGGCCT	1 cut(s)	1096
PctI	GAATGC	1 cut(s)	1158
PdmI	GAANNNNTTC	3 cut(s)	631, 1068, 1448
PfeI	GAWTC	3 cut(s)	796, 1169, 1327
PinAI	ACCGGT	1 cut(s)	998
PkrI	GCNGC	5 cut(s)	16, 103, 255, 735, 1126
PleI	GAGTC	5 cut(s)	420, 778, 923, 1167, 1433
PpsI	GAGTC	5 cut(s)	420, 778, 923, 1167, 1433
PsiI	TTATAA	1 cut(s)	26
Psp6I	CCWGG	2 cut(s)	1009, 1581
PspGI	CCWGG	2 cut(s)	1009, 1581
PspN4I	GGNNCC	3 cut(s)	1179, 1311, 1534
PspPI	GGNCC	3 cut(s)	545, 753, 1200
PsuI	RGATCY	1 cut(s)	1084
RsaI	GTAC	5 cut(s)	270, 398, 810, 1287, 1486
RsaNI	GTAC	5 cut(s)	269, 397, 809, 1286, 1485
RseI	CAYNNNNRTG	2 cut(s)	141, 1463
SaqAI	TTAA	6 cut(s)	776, 851, 888, 900, 960, 1023
SatI	GCNGC	5 cut(s)	15, 102, 254, 734, 1125
Sau3AI	GATC	4 cut(s)	856, 1084, 1261, 1409
Sau96I	GGNCC	3 cut(s)	545, 753, 1200
ScaI	AGTACT	1 cut(s)	398
SchI	GAGTC	5 cut(s)	421, 779, 924, 1168, 1433
ScrFI	CCNGG	2 cut(s)	1011, 1583
SduI	GDGCHC	2 cut(s)	363, 1537
SexAI	ACCWGGT	1 cut(s)	1581
SfaNI	GCATC	4 cut(s)	1023, 1111, 1234, 1339
SfuI	TTCGAA	1 cut(s)	674
SinI	GGWCC	1 cut(s)	753
SmiMI	CAYNNNNRTG	2 cut(s)	141, 1463
SpeI	ACTAGT	1 cut(s)	1481
SphI	GCATGC	1 cut(s)	733
Sse9I	AATT	4 cut(s)	573, 761, 954, 1357
SseBI	AGGCCT	1 cut(s)	1096
SsiI	CCGC	4 cut(s)	14, 101, 782, 1402
SspI	AATATT	1 cut(s)	1069
SspMI	CTAG	4 cut(s)	203, 290, 1073, 1482
StuI	AGGCCT	1 cut(s)	1096
StyD4I	CCNGG	2 cut(s)	1009, 1581
StyI	CCWWGG	1 cut(s)	465
TaaI	ACNGT	4 cut(s)	379, 401, 1196, 1563
TaiI	ACGT	1 cut(s)	907
TaqI	TCGA	7 cut(s)	53, 147, 321, 674, 876, 1260, 1442
TasI	AATT	4 cut(s)	573, 761, 954, 1357
TatI	WGTACW	3 cut(s)	396, 808, 1484
TauI	GCSGC	2 cut(s)	17, 104
TfiI	GAWTC	3 cut(s)	796, 1169, 1327
Tru1I	TTAA	6 cut(s)	776, 851, 888, 900, 960, 1023
Tru9I	TTAA	6 cut(s)	776, 851, 888, 900, 960, 1023
TscAI	CASTG	7 cut(s)	130, 496, 616, 658, 1111, 1411, 1498
TseFI	GTSAC	3 cut(s)	373, 862, 1493
TseI	GCWGC	3 cut(s)	253, 733, 1124
Tsp45I	GTSAC	3 cut(s)	373, 862, 1493
TspGWI	ACGGA	2 cut(s)	10, 167
TspRI	CASTG	7 cut(s)	130, 496, 616, 658, 1111, 1411, 1498
VpaK11BI	GGWCC	1 cut(s)	753
XagI	CCTNNNNNAGG	1 cut(s)	1524
XapI	RAATTY	2 cut(s)	954, 1357
XceI	RCATGY	1 cut(s)	733
XmnI	GAANNNNTTC	3 cut(s)	631, 1068, 1448
XspI	CTAG	4 cut(s)	203, 290, 1073, 1482
ZrmI	AGTACT	1 cut(s)	398
Zsp2I	ATGCAT	1 cut(s)	283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.