AT5G06230

trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009484
Physical Location & Seq
Reverse (-)
1884928 .. 1887175
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06230.3

Sequence Viewer

Length: 1242 bp
ATGGATCATCATCAACTCTTCTCTCTCTGCTCATTTTCGTACATCTTCAAGATCAAGAAACATCTCTTCGTCAGCCTCTTTTTACTTAGCCTCCTCATATTCTCGACCGTGGTCGTCGACGTCATGCCCTCTCTTCGTATCGGATTGTTGTCATCGTCATCATCGCAAACAGTAACAAAGGAGTGCGATTACTCCAAAGGAAAATGGGTTCGTCGAGCATCATCATCATCATCATCCGTTAATGGATTGTTTTATGGCGAAGAGTGTCGCTTTCTTGACTCTGGTTTTCGTTGTCATAAGCATGGAAGAAAAGATTCTGGTTATCTTGATTGGCGATGGCAACCACACGGCTGCGATCTTCCACGATTTAACGCAAGTGATTTACTTGAGAGGAGTCGGAACGGGAGGATAGTGTTCGTGGGAGATTCCATCGGAAGAAACCAGTGGGAATCCCTTATGTGCATGCTCTCACAAGCAATACCTAATAAATCTGAGATCTATGAAGTAAATGGGAACCCTATAACTAAACACAAGGGCTTTCTCTCCATGCGATTCCCTCGGGAAAACCTCACTGTTGAGTATCACAGATCACCCTTTCTTGTCGTGATTGGTCGGCCACCGGATAAATCACCGAAAGAGATCAAAACCACAGTGAGAGTTGATGAGTTTAACTGGCAGTCGAAAAGATGGGTCGGGTCAGACGTTCTGGTCTTCAATTCAGGGCATTGGTGGAACGAAGACAAAACTGTGTTAACGGGATGCTATTTTGAGGAGGGAAGGAAAGTGAACAAGACAATGGGAGTAATGGAGGCATTTGGAAAGTCTTTGAAGACATGGAAGTCATGGGTCTTGGAGAAACTTGATCCTGACAAGAGTTATGTCTTCTTCAGAAGCTACTCTCCAGTGCACTACAGAAATGGGACATGGAATACCGGTGGTCTATGTGATGCAGAGATTGAGCCTGAGACTGATAAAAGGAAGCTGGAACCTGATGCGAGTCACAATGAATATATCTATAAAGTAATTGAAGAAATGAGATATCGACATAGTAAGGTTAAGTTTCTGAACATTACATATCTGACAGAGTTTAGGAAAGATGGTCATATTTCTCGGTATCGAGAGCAAGGCACTTCTGTTGATGTTCCTCAAGACTGTAGCCACTGGTGCCTACCCGGTGTACCAGATACATGGAATGAGATCCTCTATGCGCAACTCTTGTCAATGAACTATAGAACAAAGTGA

Protein Analysis

413

Amino Acids

48.14

Weight (kDa)

8.98

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 60 - 119 5.3e-19 PMR5 N terminal Domain
PC-Esterase PF13839 120 - 308 7.6e-61 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11570 AT5G06230 AT5G06230 AT5G06230
fragaria_vesca FvH4_6g19550 FvH4_6g19550 FvH4_6g19550 FvH4_6g19550
malus_domestica MD12G1031900.v1.1 MD14G1032200.v1.1
prunus_persica Prupe.7G100700_v2.0.a1
pyrus_communis pycom12g02960
rosa_chinensis RchiOBHm_Chr3g0474891
rosa_laevigata RLG00000023883
rosa_multiflora Rmu_sc0008866.1_g000002
rosa_roxburghii Rroxscaffold_6G00406760
rosa_rugosa Rorug03G0145200
rosa_samantha Rh3AG194800 Rh3BG224700 Rh3CG219700 Rh3DG220300
rosa_wichuraiana Rw3G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 707, 838
AatII GACGTC 1 cut(s) 123
Acc16I TGCGCA 1 cut(s) 1209
AccB1I GGYRCC 1 cut(s) 1164
AccI GTMKAC 1 cut(s) 117
AclWI GGATC 3 cut(s) 12, 857, 1192
AcoI YGGCCR 1 cut(s) 614
AcuI CTGAAG 1 cut(s) 871
AcyI GRCGYC 1 cut(s) 120
AfaI GTAC 2 cut(s) 41, 1179
AgeI ACCGGT 1 cut(s) 932
AgsI TTSAA 4 cut(s) 49, 715, 829, 1028
AloI GAACNNNNNNTCC 2 cut(s) 192, 224
AluBI AGCT 2 cut(s) 894, 982
AluI AGCT 2 cut(s) 894, 982
Alw21I GWGCWC 1 cut(s) 909
Alw26I GTCTC 1 cut(s) 959
Alw44I GTGCAC 1 cut(s) 905
AlwI GGATC 3 cut(s) 12, 857, 1192
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 1 cut(s) 614
ApaLI GTGCAC 1 cut(s) 905
ApeKI GCWGC 1 cut(s) 351
AsiGI ACCGGT 1 cut(s) 932
Asp700I GAANNNNTTC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 1210
AsuC2I CCSGG 1 cut(s) 1173
AsuHPI GGTGA 2 cut(s) 582, 621
AvaI CYCGRG 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 909
BanI GGYRCC 1 cut(s) 1164
BbsI GAAGAC 4 cut(s) 703, 744, 836, 874
Bbv12I GWGCWC 1 cut(s) 909
BbvI GCAGC 1 cut(s) 338
BccI CCATC 4 cut(s) 330, 437, 681, 1091
BceAI ACGGC 1 cut(s) 364
BcnI CCSGG 1 cut(s) 1173
BcoDI GTCTC 1 cut(s) 959
BfmI CTRYAG 3 cut(s) 910, 1153, 1228
BglII AGATCT 1 cut(s) 495
BisI GCNGC 1 cut(s) 352
BlsI GCNGC 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 1173
BmeT110I CYCGRG 1 cut(s) 558
BmiI GGNNCC 3 cut(s) 515, 987, 1166
BmrFI CCNGG 1 cut(s) 1173
BmsI GCATC 4 cut(s) 227, 749, 937, 982
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 4 cut(s) 703, 744, 836, 874
BpmI CTGGAG 1 cut(s) 885
BpuEI CTTGAG 2 cut(s) 407, 1131
BpuMI CCSGG 1 cut(s) 1173
BsaBI GATNNNNATC 1 cut(s) 9
BsaHI GRCGYC 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 108, 557
BsaWI WCCGGW 2 cut(s) 619, 932
BsaXI ACNNNNNCTCC 2 cut(s) 75, 105
Bse118I RCCGGY 1 cut(s) 932
Bse1I ACTGG 4 cut(s) 442, 677, 902, 1166
Bse8I GATNNNNATC 1 cut(s) 9
BseDI CCNNGG 2 cut(s) 108, 557
BseGI GGATG 2 cut(s) 233, 764
BseJI GATNNNNATC 1 cut(s) 9
BseMII CTCAG 2 cut(s) 483, 954
BseNI ACTGG 4 cut(s) 442, 677, 902, 1166
BseRI GAGGAG 3 cut(s) 83, 406, 785
BseSI GKGCMC 1 cut(s) 909
BseXI GCAGC 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 1 cut(s) 616
BshNI GGYRCC 1 cut(s) 1164
BshTI ACCGGT 1 cut(s) 932
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 1 cut(s) 909
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 3 cut(s) 620, 933, 1173
BslFI GGGAC 1 cut(s) 934
BsmAI GTCTC 1 cut(s) 959
BsmFI GGGAC 1 cut(s) 934
BsnI GGCC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 909
Bsp143I GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
BspANI GGCC 1 cut(s) 616
BspCNI CTCAG 2 cut(s) 484, 955
BspLI GGNNCC 3 cut(s) 515, 987, 1166
BspPI GGATC 3 cut(s) 12, 857, 1192
BspT107I GGYRCC 1 cut(s) 1164
BsrFI RCCGGY 1 cut(s) 932
BsrI ACTGG 4 cut(s) 442, 677, 902, 1166
BssAI RCCGGY 1 cut(s) 932
BssECI CCNNGG 2 cut(s) 108, 557
BssMI GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
BssNI GRCGYC 1 cut(s) 120
Bst4CI ACNGT 6 cut(s) 109, 172, 574, 652, 748, 1154
Bst6I CTCTTC 4 cut(s) 23, 71, 138, 255
BstACI GRCGYC 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 464
BstDEI CTNAG 3 cut(s) 86, 492, 963
BstDSI CCRYGG 1 cut(s) 108
BstF5I GGATG 2 cut(s) 233, 764
BstHHI GCGC 1 cut(s) 1210
BstKTI GATC 8 cut(s) 7, 54, 358, 498, 590, 642, 865, 1200
BstMAI GTCTC 1 cut(s) 959
BstMBI GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 1 cut(s) 1164
BstNSI RCATGY 1 cut(s) 466
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 1171
BstSFI CTRYAG 3 cut(s) 910, 1153, 1228
BstSLI GKGCMC 1 cut(s) 909
BstV1I GCAGC 1 cut(s) 338
BstV2I GAAGAC 4 cut(s) 703, 744, 836, 874
BstX2I RGATCY 2 cut(s) 495, 1197
BstXI CCANNNNNNTGG 1 cut(s) 1188
BstYI RGATCY 2 cut(s) 495, 1197
BsuRI GGCC 1 cut(s) 616
BtgI CCRYGG 1 cut(s) 108
BtgZI GCGATG 2 cut(s) 147, 349
BtsCI GGATG 2 cut(s) 233, 764
BtsIMutI CAGTG 5 cut(s) 449, 570, 657, 909, 1159
Cac8I GCNNGC 1 cut(s) 464
CfoI GCGC 1 cut(s) 1210
Cfr10I RCCGGY 1 cut(s) 932
Csp6I GTAC 2 cut(s) 40, 1178
CspAI ACCGGT 1 cut(s) 932
CviAII CATG 8 cut(s) 124, 302, 463, 547, 834, 843, 924, 1188
CviJI RGCY 9 cut(s) 75, 90, 351, 537, 616, 894, 961, 982, 1158
CviKI_1 RGCY 9 cut(s) 75, 90, 351, 537, 616, 894, 961, 982, 1158
CviQI GTAC 2 cut(s) 40, 1178
DdeI CTNAG 3 cut(s) 86, 492, 963
DpnI GATC 8 cut(s) 6, 53, 357, 497, 589, 641, 864, 1199
DpnII GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
DrdI GACNNNNNNGTC 2 cut(s) 707, 838
DseDI GACNNNNNNGTC 2 cut(s) 707, 838
EaeI YGGCCR 1 cut(s) 614
Eam1104I CTCTTC 4 cut(s) 23, 71, 138, 255
EarI CTCTTC 4 cut(s) 23, 71, 138, 255
Eco32I GATATC 1 cut(s) 1040
Eco57I CTGAAG 1 cut(s) 871
Eco88I CYCGRG 1 cut(s) 558
EcoRV GATATC 1 cut(s) 1040
FaeI CATG 8 cut(s) 127, 305, 466, 550, 837, 846, 927, 1191
FaqI GGGAC 1 cut(s) 934
FatI CATG 8 cut(s) 123, 301, 462, 546, 833, 842, 923, 1187
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 1 cut(s) 352
FokI GGATG 2 cut(s) 220, 771
Fsp4HI GCNGC 1 cut(s) 352
FspI TGCGCA 1 cut(s) 1209
GlaI GCGC 1 cut(s) 1209
GluI GCNGC 1 cut(s) 352
GsuI CTGGAG 1 cut(s) 885
HaeIII GGCC 1 cut(s) 616
HapII CCGG 3 cut(s) 620, 933, 1173
HhaI GCGC 1 cut(s) 1210
Hin1I GRCGYC 1 cut(s) 120
Hin1II CATG 8 cut(s) 127, 305, 466, 550, 837, 846, 927, 1191
Hin6I GCGC 1 cut(s) 1208
HinP1I GCGC 1 cut(s) 1208
HincII GTYRAC 2 cut(s) 118, 753
HindII GTYRAC 2 cut(s) 118, 753
HinfI GANTC 7 cut(s) 278, 314, 394, 425, 449, 552, 997
HpaI GTTAAC 1 cut(s) 753
HpaII CCGG 3 cut(s) 620, 933, 1173
HphI GGTGA 2 cut(s) 582, 621
Hpy166II GTNNAC 5 cut(s) 118, 753, 787, 907, 1178
Hpy188I TCNGA 8 cut(s) 143, 399, 434, 493, 700, 890, 1065, 1080
Hpy8I GTNNAC 5 cut(s) 118, 753, 787, 907, 1178
Hpy99I CGWCG 3 cut(s) 119, 122, 216
HpyAV CCTTC 1 cut(s) 771
HpyCH4III ACNGT 6 cut(s) 109, 172, 574, 652, 748, 1154
HpyCH4IV ACGT 2 cut(s) 120, 702
HpyCH4V TGCA 3 cut(s) 462, 907, 950
HpyF10VI GCNNNNNNNGC 1 cut(s) 1164
HpyF3I CTNAG 3 cut(s) 86, 492, 963
HpySE526I ACGT 2 cut(s) 120, 702
Hsp92I GRCGYC 1 cut(s) 120
Hsp92II CATG 8 cut(s) 127, 305, 466, 550, 837, 846, 927, 1191
HspAI GCGC 1 cut(s) 1208
KspAI GTTAAC 1 cut(s) 753
Kzo9I GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
Lsp1109I GCAGC 1 cut(s) 338
LweI GCATC 4 cut(s) 227, 749, 937, 982
MaeII ACGT 2 cut(s) 120, 702
MaeIII GTNAC 2 cut(s) 172, 998
MalI GATC 8 cut(s) 6, 53, 357, 497, 589, 641, 864, 1199
MboI GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
MflI RGATCY 2 cut(s) 495, 1197
MhlI GDGCHC 1 cut(s) 909
MluCI AATT 2 cut(s) 715, 1023
MlyI GAGTC 3 cut(s) 272, 403, 1006
MmeI TCCRAC 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 313
MseI TTAA 5 cut(s) 240, 369, 669, 752, 1056
MslI CAYNNNNRTG 1 cut(s) 300
MspI CCGG 3 cut(s) 620, 933, 1173
MspR9I CCNGG 1 cut(s) 1173
MwoI GCNNNNNNNGC 1 cut(s) 1164
NciI CCSGG 1 cut(s) 1173
NdeII GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
NlaIII CATG 8 cut(s) 127, 305, 466, 550, 837, 846, 927, 1191
NlaIV GGNNCC 3 cut(s) 515, 987, 1166
NmuCI GTSAC 1 cut(s) 998
NsbI TGCGCA 1 cut(s) 1209
NspI RCATGY 1 cut(s) 466
PaeI GCATGC 1 cut(s) 466
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PdmI GAANNNNTTC 1 cut(s) 313
PfeI GAWTC 4 cut(s) 314, 425, 449, 552
PinAI ACCGGT 1 cut(s) 932
PkrI GCNGC 1 cut(s) 353
PleI GAGTC 3 cut(s) 272, 402, 1005
PpsI GAGTC 3 cut(s) 272, 402, 1005
PshAI GACNNNNGTC 1 cut(s) 110
PspN4I GGNNCC 3 cut(s) 515, 987, 1166
PsuI RGATCY 2 cut(s) 495, 1197
RsaI GTAC 2 cut(s) 41, 1179
RsaNI GTAC 2 cut(s) 40, 1178
RseI CAYNNNNRTG 1 cut(s) 300
SalI GTCGAC 1 cut(s) 116
SaqAI TTAA 5 cut(s) 240, 369, 669, 752, 1056
SatI GCNGC 1 cut(s) 352
Sau3AI GATC 8 cut(s) 4, 51, 355, 495, 587, 639, 862, 1197
SchI GAGTC 3 cut(s) 272, 403, 1006
ScrFI CCNGG 1 cut(s) 1173
SduI GDGCHC 1 cut(s) 909
SetI ASST 8 cut(s) 123, 484, 570, 705, 896, 984, 991, 1056
SfaNI GCATC 4 cut(s) 227, 749, 937, 982
SfcI CTRYAG 3 cut(s) 910, 1153, 1228
SgrDI CGTCGACG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 300
SmlI CTYRAG 2 cut(s) 386, 1146
SmoI CTYRAG 2 cut(s) 386, 1146
SphI GCATGC 1 cut(s) 466
Sse9I AATT 2 cut(s) 715, 1023
StyD4I CCNGG 1 cut(s) 1171
TaaI ACNGT 6 cut(s) 109, 172, 574, 652, 748, 1154
TaiI ACGT 2 cut(s) 123, 705
TaqI TCGA 6 cut(s) 104, 117, 214, 680, 1042, 1117
TasI AATT 2 cut(s) 715, 1023
TfiI GAWTC 4 cut(s) 314, 425, 449, 552
Tru1I TTAA 5 cut(s) 240, 369, 669, 752, 1056
Tru9I TTAA 5 cut(s) 240, 369, 669, 752, 1056
TscAI CASTG 5 cut(s) 449, 577, 657, 909, 1166
TseFI GTSAC 1 cut(s) 998
TseI GCWGC 1 cut(s) 351
Tsp45I GTSAC 1 cut(s) 998
TspDTI ATGAA 3 cut(s) 516, 1020, 1238
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 5 cut(s) 449, 577, 657, 909, 1166
VneI GTGCAC 1 cut(s) 905
XceI RCATGY 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 117
XmnI GAANNNNTTC 1 cut(s) 313
ZraI GACGTC 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.