Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009484

Physical Location & Seq

Genomic Coordinates Reverse (-)

3486955 .. 3488641

Gene Structure 1687 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1031900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 963 bp

ATGGATCTCCAACAATCTCATCAGCCTAAGAAGCTCCAAAATTTCCCCTTTCCATTTCTAATCAGAACGGAACTTCGTTACGCAATCTCTTTCTTCCTTCTCCTAGTTTCTTCCTTGTTTATTCTCAGTCTCGTAACCCCATTCGACTCTCAGTTTCTAGCCGATCATTTGGGTTTCTTATCCCAAATCCTACCAAAAAATAATCACAAGTTGCCTCCAGGAACTTGTGATTATTCCTACGGCAGATGGGTCCGCGACGAGACTCATCCACTTCAGTCCTACACCGAGAACTGCCCGTTTCTCGACCCTGGCTTCCGGTGCAGCCAGAGTGGTCGAAAAGATGAAGGTTATCGGAGATGGCGGTGGCAACCGGCTGGCTGTGATGTTTTGAGATTTAACGCAAGCGACTTCTTGGAAAGAAGCAGAAACGGACGGATAGTGTTTGCCGGCGACTCCATCGGAAGGAACCAGTGGGAATCCATGCTATGCATGCTTGCAGAAGGGGCGTCCAACAAGTCTAGAATCTATGAAGTGAATGGGAACCCCATAACAAAACACAAGGGTTTTCTGTCGATGCGGTTCCAGGACTACAACCTCACAGTTGAATATTACAGGGCACCCTTCCTTGTGGTTGTAGGACACCCACCCAAAAATTCACCAAACCAGGTCAGGACCACAGTTAAGCTCGATCAATTCCATTGGTACTCCAAACACTGGGTTGGAGCAGATGTTCTAGTTTTCAGTGCAGGGCACTGGTGGAACGAAGGCAAAACAGCAAAGATGGGCTGCTACTTTGAGGAAGACGGCAAAGTGAATTTGACAATGGGAGCGATGGAAGCATTCAGGAGGTCTATACAAACCTGGAAATCTTGGGTGCTCAAGAATTTAGACCCTCGAAGGACTCAGGTTTTCTACCGAAGCTTCTCTCCGGTGCATTACAGGCAAGTATCAACAGATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

321

Amino Acids

37.41

Weight (kDa)

9.39

Isoelectric Point (pI)

53.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	75 - 128	4.2e-18	PMR5 N terminal Domain
PC-Esterase	PF13839	131 - 316	3.3e-58	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009484)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11570 AT5G06230 AT5G06230 AT5G06230
fragaria_vesca	FvH4_6g19550 FvH4_6g19550 FvH4_6g19550 FvH4_6g19550
malus_domestica	MD12G1031900.v1.1 MD14G1032200.v1.1
prunus_persica	Prupe.7G100700_v2.0.a1
pyrus_communis	pycom12g02960
rosa_chinensis	RchiOBHm_Chr3g0474891
rosa_laevigata	RLG00000023883
rosa_multiflora	Rmu_sc0008866.1_g000002
rosa_roxburghii	Rroxscaffold_6G00406760
rosa_rugosa	Rorug03G0145200
rosa_samantha	Rh3AG194800 Rh3BG224700 Rh3CG219700 Rh3DG220300
rosa_wichuraiana	Rw3G017730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	616
AccB7I	CCANNNNNTGG	1 cut(s)	714
AccII	CGCG	1 cut(s)	255
AciI	CCGC	3 cut(s)	253, 361, 577
AclWI	GGATC	1 cut(s)	12
AcsI	RAATTY	4 cut(s)	40, 652, 814, 883
AcuI	CTGAAG	1 cut(s)	257
AcyI	GRCGYC	1 cut(s)	506
AfaI	GTAC	1 cut(s)	704
AfiI	CCNNNNNNNGG	2 cut(s)	462, 714
AgsI	TTSAA	1 cut(s)	605
AjnI	CCWGG	5 cut(s)	217, 307, 582, 663, 860
AloI	GAACNNNNNNTCC	2 cut(s)	714, 746
AluBI	AGCT	3 cut(s)	34, 685, 921
AluI	AGCT	3 cut(s)	34, 685, 921
Alw21I	GWGCWC	1 cut(s)	879
Alw26I	GTCTC	2 cut(s)	134, 254
AlwI	GGATC	1 cut(s)	12
ApeKI	GCWGC	2 cut(s)	321, 786
ApoI	RAATTY	4 cut(s)	40, 652, 814, 883
AspS9I	GGNCC	2 cut(s)	250, 672
AsuHPI	GGTGA	1 cut(s)	648
AvaII	GGWCC	2 cut(s)	250, 672
BaeGI	GKGCMC	2 cut(s)	619, 753
BanI	GGYRCC	1 cut(s)	616
BbsI	GAAGAC	1 cut(s)	807
Bbv12I	GWGCWC	1 cut(s)	879
BbvI	GCAGC	2 cut(s)	333, 773
BccI	CCATC	5 cut(s)	240, 351, 464, 775, 826
BceAI	ACGGC	2 cut(s)	256, 820
BciT130I	CCWGG	5 cut(s)	219, 309, 584, 665, 862
BcoDI	GTCTC	2 cut(s)	134, 254
BfaI	CTAG	4 cut(s)	104, 158, 519, 734
BisI	GCNGC	2 cut(s)	322, 787
BlsI	GCNGC	2 cut(s)	323, 788
Bme1390I	CCNGG	5 cut(s)	219, 309, 584, 665, 862
Bme18I	GGWCC	2 cut(s)	250, 672
BmgT120I	GGNCC	2 cut(s)	250, 672
BmiI	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
BmrFI	CCNGG	5 cut(s)	219, 309, 584, 665, 862
BmrI	ACTGGG	1 cut(s)	724
BmsI	GCATC	1 cut(s)	564
BmuI	ACTGGG	1 cut(s)	724
BpiI	GAAGAC	1 cut(s)	807
BpmI	CTGGAG	1 cut(s)	201
BpuEI	CTTGAG	1 cut(s)	863
BsaHI	GRCGYC	1 cut(s)	506
BsaJI	CCNNGG	1 cut(s)	307
BsaWI	WCCGGW	2 cut(s)	315, 928
BsaXI	ACNNNNNCTCC	2 cut(s)	714, 744
Bsc4I	CCNNNNNNNGG	2 cut(s)	462, 714
Bse118I	RCCGGY	2 cut(s)	370, 446
Bse1I	ACTGG	3 cut(s)	469, 719, 758
BseBI	CCWGG	5 cut(s)	219, 309, 584, 665, 862
BseDI	CCNNGG	1 cut(s)	307
BseGI	GGATG	1 cut(s)	265
BseLI	CCNNNNNNNGG	2 cut(s)	462, 714
BseMII	CTCAG	3 cut(s)	139, 164, 917
BseNI	ACTGG	3 cut(s)	469, 719, 758
BseSI	GKGCMC	2 cut(s)	619, 753
BseXI	GCAGC	2 cut(s)	333, 773
BsgI	GTGCAG	2 cut(s)	340, 765
Bsh1236I	CGCG	1 cut(s)	255
BshNI	GGYRCC	1 cut(s)	616
BsiHKAI	GWGCWC	1 cut(s)	879
BsiSI	CCGG	4 cut(s)	316, 371, 447, 929
BslI	CCNNNNNNNGG	2 cut(s)	462, 714
BsmAI	GTCTC	2 cut(s)	134, 254
BsmI	GAATGC	1 cut(s)	839
Bsp1286I	GDGCHC	3 cut(s)	619, 753, 879
Bsp143I	GATC	3 cut(s)	4, 163, 688
BspACI	CCGC	3 cut(s)	253, 361, 577
BspCNI	CTCAG	3 cut(s)	138, 163, 916
BspFNI	CGCG	1 cut(s)	255
BspLI	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
BspPI	GGATC	1 cut(s)	12
BspT107I	GGYRCC	1 cut(s)	616
BsrFI	RCCGGY	2 cut(s)	370, 446
BsrI	ACTGG	3 cut(s)	469, 719, 758
BssAI	RCCGGY	2 cut(s)	370, 446
BssECI	CCNNGG	1 cut(s)	307
BssMI	GATC	3 cut(s)	4, 163, 688
BssNI	GRCGYC	1 cut(s)	506
Bst2UI	CCWGG	5 cut(s)	219, 309, 584, 665, 862
Bst4CI	ACNGT	2 cut(s)	601, 679
BstACI	GRCGYC	1 cut(s)	506
BstC8I	GCNNGC	5 cut(s)	376, 403, 448, 491, 495
BstDEI	CTNAG	4 cut(s)	27, 125, 150, 903
BstF5I	GGATG	1 cut(s)	265
BstFNI	CGCG	1 cut(s)	255
BstKTI	GATC	3 cut(s)	7, 166, 691
BstMAI	GTCTC	2 cut(s)	134, 254
BstMBI	GATC	3 cut(s)	4, 163, 688
BstMWI	GCNNNNNNNGC	6 cut(s)	31, 318, 490, 503, 836, 940
BstNI	CCWGG	5 cut(s)	219, 309, 584, 665, 862
BstNSI	RCATGY	1 cut(s)	493
BstSCI	CCNGG	5 cut(s)	217, 307, 582, 663, 860
BstSLI	GKGCMC	2 cut(s)	619, 753
BstUI	CGCG	1 cut(s)	255
BstV1I	GCAGC	2 cut(s)	333, 773
BstV2I	GAAGAC	1 cut(s)	807
BstX2I	RGATCY	1 cut(s)	4
BstYI	RGATCY	1 cut(s)	4
BtgZI	GCGATG	1 cut(s)	845
BtsCI	GGATG	1 cut(s)	265
BtsIMutI	CAGTG	4 cut(s)	476, 712, 748, 751
Cac8I	GCNNGC	5 cut(s)	376, 403, 448, 491, 495
Cfr10I	RCCGGY	2 cut(s)	370, 446
Cfr13I	GGNCC	2 cut(s)	250, 672
CseI	GACGC	1 cut(s)	495
CsiI	ACCWGGT	1 cut(s)	663
Csp6I	GTAC	1 cut(s)	703
CviAII	CATG	2 cut(s)	481, 490
CviQI	GTAC	1 cut(s)	703
DdeI	CTNAG	4 cut(s)	27, 125, 150, 903
DpnI	GATC	3 cut(s)	6, 165, 690
DpnII	GATC	3 cut(s)	4, 163, 688
Eco47I	GGWCC	2 cut(s)	250, 672
Eco57I	CTGAAG	1 cut(s)	257
EcoRII	CCWGG	5 cut(s)	217, 307, 582, 663, 860
EcoT22I	ATGCAT	1 cut(s)	491
FaeI	CATG	2 cut(s)	484, 493
FaiI	YATR	6 cut(s)	482, 487, 491, 528, 548, 854
FatI	CATG	2 cut(s)	480, 489
Fnu4HI	GCNGC	2 cut(s)	322, 787
FokI	GGATG	1 cut(s)	252
Fsp4HI	GCNGC	2 cut(s)	322, 787
FspBI	CTAG	4 cut(s)	104, 158, 519, 734
GluI	GCNGC	2 cut(s)	322, 787
GsuI	CTGGAG	1 cut(s)	201
HapII	CCGG	4 cut(s)	316, 371, 447, 929
HgaI	GACGC	1 cut(s)	495
Hin1I	GRCGYC	1 cut(s)	506
Hin1II	CATG	2 cut(s)	484, 493
HindIII	AAGCTT	1 cut(s)	919
HinfI	GANTC	6 cut(s)	146, 262, 452, 476, 522, 901
HpaII	CCGG	4 cut(s)	316, 371, 447, 929
HphI	GGTGA	1 cut(s)	648
Hpy188I	TCNGA	4 cut(s)	65, 354, 461, 962
Hpy188III	TCNNGA	5 cut(s)	302, 519, 670, 844, 880
Hpy99I	CGWCG	1 cut(s)	260
HpyAV	CCTTC	7 cut(s)	107, 338, 456, 494, 631, 758, 891
HpyCH4III	ACNGT	2 cut(s)	601, 679
HpyCH4V	TGCA	5 cut(s)	321, 489, 497, 746, 934
HpyF10VI	GCNNNNNNNGC	6 cut(s)	31, 318, 490, 503, 836, 940
HpyF3I	CTNAG	4 cut(s)	27, 125, 150, 903
Hsp92I	GRCGYC	1 cut(s)	506
Hsp92II	CATG	2 cut(s)	484, 493
KroI	GCCGGC	1 cut(s)	446
KroNI	GCCGGC	1 cut(s)	448
Kzo9I	GATC	3 cut(s)	4, 163, 688
LmnI	GCTCC	3 cut(s)	39, 722, 827
Lsp1109I	GCAGC	2 cut(s)	333, 773
LweI	GCATC	1 cut(s)	564
MabI	ACCWGGT	1 cut(s)	663
MaeI	CTAG	4 cut(s)	104, 158, 519, 734
MaeIII	GTNAC	2 cut(s)	77, 133
MalI	GATC	3 cut(s)	6, 165, 690
MboI	GATC	3 cut(s)	4, 163, 688
MboII	GAAGA	3 cut(s)	85, 102, 812
MflI	RGATCY	1 cut(s)	4
MhlI	GDGCHC	3 cut(s)	619, 753, 879
MluCI	AATT	5 cut(s)	40, 652, 692, 814, 883
MlyI	GAGTC	4 cut(s)	140, 256, 446, 895
MmeI	TCCRAC	3 cut(s)	34, 534, 700
MnlI	CCTC	5 cut(s)	225, 605, 790, 840, 903
Mph1103I	ATGCAT	1 cut(s)	491
MroNI	GCCGGC	1 cut(s)	446
MseI	TTAA	2 cut(s)	396, 681
MspI	CCGG	4 cut(s)	316, 371, 447, 929
MspR9I	CCNGG	5 cut(s)	219, 309, 584, 665, 862
Mva1269I	GAATGC	1 cut(s)	839
MvaI	CCWGG	5 cut(s)	219, 309, 584, 665, 862
MvnI	CGCG	1 cut(s)	255
MwoI	GCNNNNNNNGC	6 cut(s)	31, 318, 490, 503, 836, 940
NaeI	GCCGGC	1 cut(s)	448
NdeII	GATC	3 cut(s)	4, 163, 688
NgoMIV	GCCGGC	1 cut(s)	446
NlaIII	CATG	2 cut(s)	484, 493
NlaIV	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
NsiI	ATGCAT	1 cut(s)	491
NspI	RCATGY	1 cut(s)	493
PaeI	GCATGC	1 cut(s)	493
PctI	GAATGC	1 cut(s)	839
PdiI	GCCGGC	1 cut(s)	448
PfeI	GAWTC	2 cut(s)	476, 522
PflMI	CCANNNNNTGG	1 cut(s)	714
PfoI	TCCNGGA	2 cut(s)	217, 582
PkrI	GCNGC	2 cut(s)	323, 788
PleI	GAGTC	4 cut(s)	140, 256, 446, 895
PpsI	GAGTC	4 cut(s)	140, 256, 446, 895
Psp6I	CCWGG	5 cut(s)	217, 307, 582, 663, 860
PspGI	CCWGG	5 cut(s)	217, 307, 582, 663, 860
PspN4I	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
PspPI	GGNCC	2 cut(s)	250, 672
PsuI	RGATCY	1 cut(s)	4
RsaI	GTAC	1 cut(s)	704
RsaNI	GTAC	1 cut(s)	703
SaqAI	TTAA	2 cut(s)	396, 681
SatI	GCNGC	2 cut(s)	322, 787
Sau3AI	GATC	3 cut(s)	4, 163, 688
Sau96I	GGNCC	2 cut(s)	250, 672
SchI	GAGTC	4 cut(s)	140, 256, 446, 895
ScrFI	CCNGG	5 cut(s)	219, 309, 584, 665, 862
SduI	GDGCHC	3 cut(s)	619, 753, 879
SetI	ASST	9 cut(s)	36, 349, 597, 669, 687, 851, 863, 909, 923
SexAI	ACCWGGT	1 cut(s)	663
SfaNI	GCATC	1 cut(s)	564
SinI	GGWCC	2 cut(s)	250, 672
SmlI	CTYRAG	1 cut(s)	878
SmoI	CTYRAG	1 cut(s)	878
SphI	GCATGC	1 cut(s)	493
Sse9I	AATT	5 cut(s)	40, 652, 692, 814, 883
SsiI	CCGC	3 cut(s)	253, 361, 577
SspI	AATATT	1 cut(s)	608
SspMI	CTAG	4 cut(s)	104, 158, 519, 734
StyD4I	CCNGG	5 cut(s)	217, 307, 582, 663, 860
TaaI	ACNGT	2 cut(s)	601, 679
TaqI	TCGA	6 cut(s)	144, 303, 334, 572, 687, 895
TasI	AATT	5 cut(s)	40, 652, 692, 814, 883
TfiI	GAWTC	2 cut(s)	476, 522
Tru1I	TTAA	2 cut(s)	396, 681
Tru9I	TTAA	2 cut(s)	396, 681
TscAI	CASTG	4 cut(s)	476, 719, 748, 758
TseI	GCWGC	2 cut(s)	321, 786
TspDTI	ATGAA	2 cut(s)	357, 543
TspGWI	ACGGA	3 cut(s)	83, 444, 448
TspRI	CASTG	4 cut(s)	476, 719, 748, 758
Van91I	CCANNNNNTGG	1 cut(s)	714
VpaK11BI	GGWCC	2 cut(s)	250, 672
XapI	RAATTY	4 cut(s)	40, 652, 814, 883
XbaI	TCTAGA	1 cut(s)	518
XceI	RCATGY	1 cut(s)	493
XspI	CTAG	4 cut(s)	104, 158, 519, 734
Zsp2I	ATGCAT	1 cut(s)	491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.