Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009484

Physical Location & Seq

Genomic Coordinates Reverse (-)

2879608 .. 2881166

Gene Structure 1559 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1032200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 969 bp

ATGGATCTCCAACCCTCTCATCAACCTCAGAAGCTTCATAATTTCCCCTTTTCATTTCTAATCGGAAGAGAACTTCGTTACGGACTCTCTTTCTTCCTTCTCCTCGTCTCTTCCTTGTTTATTCTCAGACTCGTAACCCCATTCGACTCTCAGTTTCTATCCGATCATTTGGGTTTCTTATCCCAAATCCTACAGAATAATAATCAAAATTTGTCTCCGGGAACTTGTGATTATTCCTACGGCAGATGGGTCCACGACGAAACTCATCCGCTTCAGTCCTACACCGAGGACTGCCCGTTTCTCGACCCTGGCTTCCGGTGCAGCCAGAGTGGTCGAAAAGACCAAGGTTATCGGAGATGGCGCTGGCAACCGGCTGGCTGTGACGTTTTGAGATTTAACGCAAGCGACTTCTTGGAAAGAAGCAGAAACGGACGGATAGTATTTGCCGGAGACTCAATCGGGAGGAACCAGTGGGAATCTATGCTATGCATGCTTGCGAAAGGGGTGTCCAAGAAGTCCAGAATCTACGAAGTAAATGGGAACCCCATAACAAAACATAGGGGTTTTCTGTCGATACGGTTCCAGGACTATAACCTCACAGTTGAATATTATAGGGCACCGTTCCTTGTGGTTGTAGGACACCCACCCAAAAATTCACCAAGTCAGGTCAGGACCACGGTTAAGCTCGACCAAATCCATTGGTACTCCGAAAAATGGGTTGGAGCAGATGTTCTAATTTTCAGTGCAGGGCACTGGTGGAACGAAGACAAAACAGCAAAGATGGGCTGCTACTTTGAGGAAGACGGGAAAGTGAATCTGACAATGGGTGTTATGGAAGCATTCAGGAGGTCTATACAAACATGGAAGTCTTGGGTGCTCAAGAATTTAGACCCTGAAAGGACTCGGGTTTTCTTCCGAAGCTACTCTCCGGTACATTACAGGCAAGTACAAACTGCTATCCGACTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

323

Amino Acids

37.69

Weight (kDa)

9.34

Isoelectric Point (pI)

52.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	75 - 128	7.3e-18	PMR5 N terminal Domain
PC-Esterase	PF13839	131 - 316	5.6e-60	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009484)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11570 AT5G06230 AT5G06230 AT5G06230
fragaria_vesca	FvH4_6g19550 FvH4_6g19550 FvH4_6g19550 FvH4_6g19550
malus_domestica	MD12G1031900.v1.1 MD14G1032200.v1.1
prunus_persica	Prupe.7G100700_v2.0.a1
pyrus_communis	pycom12g02960
rosa_chinensis	RchiOBHm_Chr3g0474891
rosa_laevigata	RLG00000023883
rosa_multiflora	Rmu_sc0008866.1_g000002
rosa_roxburghii	Rroxscaffold_6G00406760
rosa_rugosa	Rorug03G0145200
rosa_samantha	Rh3AG194800 Rh3BG224700 Rh3CG219700 Rh3DG220300
rosa_wichuraiana	Rw3G017730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	616
AciI	CCGC	1 cut(s)	269
AclWI	GGATC	1 cut(s)	12
AcsI	RAATTY	3 cut(s)	208, 652, 883
AcuI	CTGAAG	1 cut(s)	257
AfaI	GTAC	3 cut(s)	704, 933, 948
AfiI	CCNNNNNNNGG	1 cut(s)	714
AgsI	TTSAA	1 cut(s)	605
AjnI	CCWGG	2 cut(s)	307, 582
AjuI	GAANNNNNNNTTGG	2 cut(s)	702, 734
AloI	GAACNNNNNNTCC	2 cut(s)	714, 746
AluBI	AGCT	3 cut(s)	34, 685, 921
AluI	AGCT	3 cut(s)	34, 685, 921
Alw21I	GWGCWC	1 cut(s)	879
Alw26I	GTCTC	3 cut(s)	112, 219, 444
AlwI	GGATC	1 cut(s)	12
Ama87I	CYCGRG	1 cut(s)	903
ApeKI	GCWGC	2 cut(s)	321, 786
ApoI	RAATTY	3 cut(s)	208, 652, 883
ArsI	GACNNNNNNTTYG	2 cut(s)	491, 523
AspLEI	GCGC	1 cut(s)	363
AspS9I	GGNCC	2 cut(s)	250, 672
AsuC2I	CCSGG	1 cut(s)	219
AsuHPI	GGTGA	1 cut(s)	648
AvaI	CYCGRG	1 cut(s)	903
AvaII	GGWCC	2 cut(s)	250, 672
BaeGI	GKGCMC	2 cut(s)	619, 753
BanI	GGYRCC	1 cut(s)	616
BbsI	GAAGAC	2 cut(s)	771, 807
Bbv12I	GWGCWC	1 cut(s)	879
BbvI	GCAGC	2 cut(s)	333, 773
BccI	CCATC	3 cut(s)	240, 351, 775
BceAI	ACGGC	1 cut(s)	256
BciT130I	CCWGG	2 cut(s)	309, 584
BcnI	CCSGG	1 cut(s)	219
BcoDI	GTCTC	3 cut(s)	112, 219, 444
BfmI	CTRYAG	1 cut(s)	191
BfoI	RGCGCY	1 cut(s)	364
BisI	GCNGC	2 cut(s)	322, 787
BlsI	GCNGC	2 cut(s)	323, 788
Bme1390I	CCNGG	3 cut(s)	219, 309, 584
Bme18I	GGWCC	2 cut(s)	250, 672
BmeT110I	CYCGRG	1 cut(s)	903
BmgT120I	GGNCC	2 cut(s)	250, 672
BmiI	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
BmrFI	CCNGG	3 cut(s)	219, 309, 584
BpiI	GAAGAC	2 cut(s)	771, 807
BpuEI	CTTGAG	1 cut(s)	863
BpuMI	CCSGG	1 cut(s)	219
BsaJI	CCNNGG	4 cut(s)	285, 307, 343, 675
BsaWI	WCCGGW	2 cut(s)	315, 928
BsaXI	ACNNNNNCTCC	2 cut(s)	714, 744
Bsc4I	CCNNNNNNNGG	1 cut(s)	714
Bse118I	RCCGGY	1 cut(s)	370
Bse1I	ACTGG	2 cut(s)	469, 758
BseBI	CCWGG	2 cut(s)	309, 584
BseDI	CCNNGG	4 cut(s)	285, 307, 343, 675
BseGI	GGATG	1 cut(s)	265
BseLI	CCNNNNNNNGG	1 cut(s)	714
BseMII	CTCAG	3 cut(s)	41, 139, 164
BseNI	ACTGG	2 cut(s)	469, 758
BseRI	GAGGAG	1 cut(s)	92
BseSI	GKGCMC	2 cut(s)	619, 753
BseXI	GCAGC	2 cut(s)	333, 773
BsgI	GTGCAG	2 cut(s)	340, 765
BshNI	GGYRCC	1 cut(s)	616
BsiHKAI	GWGCWC	1 cut(s)	879
BsiHKCI	CYCGRG	1 cut(s)	903
BsiSI	CCGG	5 cut(s)	218, 316, 371, 447, 929
BslI	CCNNNNNNNGG	1 cut(s)	714
BsmAI	GTCTC	3 cut(s)	112, 219, 444
BsmBI	CGTCTC	1 cut(s)	112
BsmI	GAATGC	1 cut(s)	839
BsoBI	CYCGRG	1 cut(s)	903
Bsp1286I	GDGCHC	3 cut(s)	619, 753, 879
Bsp143I	GATC	2 cut(s)	4, 163
BspACI	CCGC	1 cut(s)	269
BspCNI	CTCAG	3 cut(s)	40, 138, 163
BspLI	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
BspPI	GGATC	1 cut(s)	12
BspT107I	GGYRCC	1 cut(s)	616
BsrFI	RCCGGY	1 cut(s)	370
BsrI	ACTGG	2 cut(s)	469, 758
BssAI	RCCGGY	1 cut(s)	370
BssECI	CCNNGG	4 cut(s)	285, 307, 343, 675
BssMI	GATC	2 cut(s)	4, 163
BssT1I	CCWWGG	1 cut(s)	343
Bst2UI	CCWGG	2 cut(s)	309, 584
Bst4CI	ACNGT	4 cut(s)	579, 601, 621, 679
Bst6I	CTCTTC	2 cut(s)	61, 115
BstC8I	GCNNGC	5 cut(s)	365, 376, 403, 491, 495
BstDEI	CTNAG	3 cut(s)	27, 125, 150
BstDSI	CCRYGG	1 cut(s)	675
BstF5I	GGATG	1 cut(s)	265
BstH2I	RGCGCY	1 cut(s)	364
BstHHI	GCGC	1 cut(s)	363
BstKTI	GATC	2 cut(s)	7, 166
BstMAI	GTCTC	3 cut(s)	112, 219, 444
BstMBI	GATC	2 cut(s)	4, 163
BstMWI	GCNNNNNNNGC	2 cut(s)	318, 490
BstNI	CCWGG	2 cut(s)	309, 584
BstNSI	RCATGY	1 cut(s)	493
BstSCI	CCNGG	3 cut(s)	217, 307, 582
BstSFI	CTRYAG	1 cut(s)	191
BstSLI	GKGCMC	2 cut(s)	619, 753
BstV1I	GCAGC	2 cut(s)	333, 773
BstV2I	GAAGAC	2 cut(s)	771, 807
BstX2I	RGATCY	1 cut(s)	4
BstYI	RGATCY	1 cut(s)	4
BtgI	CCRYGG	1 cut(s)	675
BtsCI	GGATG	1 cut(s)	265
BtsIMutI	CAGTG	3 cut(s)	476, 748, 751
Cac8I	GCNNGC	5 cut(s)	365, 376, 403, 491, 495
CfoI	GCGC	1 cut(s)	363
Cfr10I	RCCGGY	1 cut(s)	370
Cfr13I	GGNCC	2 cut(s)	250, 672
Csp6I	GTAC	3 cut(s)	703, 932, 947
CviAII	CATG	2 cut(s)	490, 861
CviJI	RGCY	8 cut(s)	34, 312, 324, 374, 378, 685, 786, 921
CviKI_1	RGCY	8 cut(s)	34, 312, 324, 374, 378, 685, 786, 921
CviQI	GTAC	3 cut(s)	703, 932, 947
DdeI	CTNAG	3 cut(s)	27, 125, 150
DpnI	GATC	2 cut(s)	6, 165
DpnII	GATC	2 cut(s)	4, 163
Eam1104I	CTCTTC	2 cut(s)	61, 115
EarI	CTCTTC	2 cut(s)	61, 115
Eco130I	CCWWGG	1 cut(s)	343
Eco47I	GGWCC	2 cut(s)	250, 672
Eco57I	CTGAAG	1 cut(s)	257
Eco88I	CYCGRG	1 cut(s)	903
EcoRII	CCWGG	2 cut(s)	307, 582
EcoT14I	CCWWGG	1 cut(s)	343
EcoT22I	ATGCAT	1 cut(s)	491
ErhI	CCWWGG	1 cut(s)	343
Esp3I	CGTCTC	1 cut(s)	112
FaeI	CATG	2 cut(s)	493, 864
FatI	CATG	2 cut(s)	489, 860
Fnu4HI	GCNGC	2 cut(s)	322, 787
FokI	GGATG	1 cut(s)	252
Fsp4HI	GCNGC	2 cut(s)	322, 787
GlaI	GCGC	1 cut(s)	362
GluI	GCNGC	2 cut(s)	322, 787
HaeII	RGCGCY	1 cut(s)	364
HapII	CCGG	5 cut(s)	218, 316, 371, 447, 929
HhaI	GCGC	1 cut(s)	363
Hin1II	CATG	2 cut(s)	493, 864
Hin6I	GCGC	1 cut(s)	361
HinP1I	GCGC	1 cut(s)	361
HindIII	AAGCTT	1 cut(s)	32
HinfI	GANTC	8 cut(s)	84, 129, 146, 452, 476, 522, 814, 901
HpaII	CCGG	5 cut(s)	218, 316, 371, 447, 929
HphI	GGTGA	1 cut(s)	648
Hpy166II	GTNNAC	1 cut(s)	253
Hpy188I	TCNGA	9 cut(s)	30, 65, 128, 163, 354, 709, 819, 917, 962
Hpy188III	TCNNGA	6 cut(s)	302, 460, 519, 670, 844, 880
Hpy8I	GTNNAC	1 cut(s)	253
Hpy99I	CGWCG	1 cut(s)	260
HpyAV	CCTTC	1 cut(s)	107
HpyCH4III	ACNGT	4 cut(s)	579, 601, 621, 679
HpyCH4IV	ACGT	1 cut(s)	384
HpyCH4V	TGCA	3 cut(s)	321, 489, 746
HpyF10VI	GCNNNNNNNGC	2 cut(s)	318, 490
HpyF3I	CTNAG	3 cut(s)	27, 125, 150
HpySE526I	ACGT	1 cut(s)	384
Hsp92II	CATG	2 cut(s)	493, 864
HspAI	GCGC	1 cut(s)	361
Kzo9I	GATC	2 cut(s)	4, 163
LmnI	GCTCC	1 cut(s)	722
Lsp1109I	GCAGC	2 cut(s)	333, 773
MaeII	ACGT	1 cut(s)	384
MaeIII	GTNAC	3 cut(s)	77, 133, 380
MalI	GATC	2 cut(s)	6, 165
MboI	GATC	2 cut(s)	4, 163
MboII	GAAGA	6 cut(s)	78, 85, 102, 776, 812, 904
MflI	RGATCY	1 cut(s)	4
MhlI	GDGCHC	3 cut(s)	619, 753, 879
MluCI	AATT	5 cut(s)	40, 208, 652, 735, 883
MlyI	GAGTC	5 cut(s)	78, 123, 140, 446, 895
MmeI	TCCRAC	2 cut(s)	34, 700
MnlI	CCTC	8 cut(s)	25, 36, 113, 280, 456, 605, 790, 840
Mph1103I	ATGCAT	1 cut(s)	491
MseI	TTAA	2 cut(s)	396, 681
MspI	CCGG	5 cut(s)	218, 316, 371, 447, 929
MspR9I	CCNGG	3 cut(s)	219, 309, 584
Mva1269I	GAATGC	1 cut(s)	839
MvaI	CCWGG	2 cut(s)	309, 584
MwoI	GCNNNNNNNGC	2 cut(s)	318, 490
NciI	CCSGG	1 cut(s)	219
NdeII	GATC	2 cut(s)	4, 163
NlaIII	CATG	2 cut(s)	493, 864
NlaIV	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
NmuCI	GTSAC	1 cut(s)	380
NsiI	ATGCAT	1 cut(s)	491
NspI	RCATGY	1 cut(s)	493
PaeI	GCATGC	1 cut(s)	493
PctI	GAATGC	1 cut(s)	839
PfeI	GAWTC	3 cut(s)	476, 522, 814
PfoI	TCCNGGA	2 cut(s)	217, 582
PkrI	GCNGC	2 cut(s)	323, 788
PleI	GAGTC	5 cut(s)	78, 123, 140, 446, 895
PpsI	GAGTC	5 cut(s)	78, 123, 140, 446, 895
Psp6I	CCWGG	2 cut(s)	307, 582
PspGI	CCWGG	2 cut(s)	307, 582
PspN4I	GGNNCC	5 cut(s)	251, 467, 542, 581, 618
PspPI	GGNCC	2 cut(s)	250, 672
PsuI	RGATCY	1 cut(s)	4
RsaI	GTAC	3 cut(s)	704, 933, 948
RsaNI	GTAC	3 cut(s)	703, 932, 947
SaqAI	TTAA	2 cut(s)	396, 681
SatI	GCNGC	2 cut(s)	322, 787
Sau3AI	GATC	2 cut(s)	4, 163
Sau96I	GGNCC	2 cut(s)	250, 672
SchI	GAGTC	5 cut(s)	78, 123, 140, 446, 895
ScrFI	CCNGG	3 cut(s)	219, 309, 584
SduI	GDGCHC	3 cut(s)	619, 753, 879
SetI	ASST	9 cut(s)	28, 36, 349, 387, 597, 669, 687, 851, 923
SfcI	CTRYAG	1 cut(s)	191
SinI	GGWCC	2 cut(s)	250, 672
SmlI	CTYRAG	1 cut(s)	878
SmoI	CTYRAG	1 cut(s)	878
SphI	GCATGC	1 cut(s)	493
Sse9I	AATT	5 cut(s)	40, 208, 652, 735, 883
SsiI	CCGC	1 cut(s)	269
SspI	AATATT	1 cut(s)	608
StyD4I	CCNGG	3 cut(s)	217, 307, 582
StyI	CCWWGG	1 cut(s)	343
TaaI	ACNGT	4 cut(s)	579, 601, 621, 679
TaiI	ACGT	1 cut(s)	387
TaqI	TCGA	5 cut(s)	144, 303, 334, 572, 687
TasI	AATT	5 cut(s)	40, 208, 652, 735, 883
TatI	WGTACW	1 cut(s)	946
TfiI	GAWTC	3 cut(s)	476, 522, 814
Tru1I	TTAA	2 cut(s)	396, 681
Tru9I	TTAA	2 cut(s)	396, 681
TscAI	CASTG	3 cut(s)	476, 748, 758
TseFI	GTSAC	1 cut(s)	380
TseI	GCWGC	2 cut(s)	321, 786
Tsp45I	GTSAC	1 cut(s)	380
TspDTI	ATGAA	2 cut(s)	26, 42
TspGWI	ACGGA	3 cut(s)	96, 444, 448
TspRI	CASTG	3 cut(s)	476, 748, 758
VpaK11BI	GGWCC	2 cut(s)	250, 672
XapI	RAATTY	3 cut(s)	208, 652, 883
XceI	RCATGY	1 cut(s)	493
Zsp2I	ATGCAT	1 cut(s)	491

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.