Rroxscaffold_6G00406760

Protein trichome birefringence-like 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0009484
Physical Location & Seq
Forward (+)
29338995 .. 29341117
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00406760.1

Sequence Viewer

Length: 1179 bp
ATGGATCTTCAACCCTCCAATCAATCTCAGAAGCCCCAAGGTTTCCCCTTTTCCATTTTTACCAGAAGAGACCTTCGTTATCCGCTCTCTTGCTTCGGTGTCTTCGTCTGTTCCATCGCTATTCTCAATCTTTTCACCCCATTTGACTCGCATGTTATATCTCGTTTGGGTTCCTTATATTCCCAAATCCTACCAGACATTCACAAATCATCATCATCCTCTGGCACTTGTGACTACTCCTATGGAAGATGGGTCCGAGACAAGAATCATGCACTTCAGTTGTACAGCGAGAACTGCCCGTTTCTCGATCCCGGCTTTCGGTGCAGCCAGAGTGGTCGGAAAGACGAAGATTATCGTCAATGGCGATGGCAACCGGCGGGCTGTGATCTTTTGAGGTTTAACGCAAGCGACTTCTTGGAGAGAAGTCGAAATGGACGAATAGTGTTTGCTGGCGACTCGATCGGAAGGAACCAGTGGGAATCTCTGCTGTGCATGCTTGCAGAAGCAGTATCCAACAAGTCTAGAATCTATGAAGTAAATGGGAACCCCATTACAAAACACAGAGGTTTTCTATCAATGCGATTCCAGGACTACAACCTCACAGTTGAATATTACAGGGCACCCTTCCTTGTGGTCGTAGGGCACCCAACTCAAAATGCATCAAGCCAAGTCAGGATCACAGTTAAGGTCGACCAATTCCACTGGTTTTCCAAACATTGGGTTGGAGCAGATGTTCTGATTTTCAGTGCAGGGCACTGGTGGAACGAGGAGAAAACAGTAAGGATGGGTCACTACTTCGAGGAAGCCGGTAAAGTGAATTTGACAATGGATGTGATGGAAGCATTCAGGAGGAATGGCACATGGAATGAAGGCGGGCTGTGTGATACCTTTACGGAGCCAGAAAGAGACCACAAACATCTTGAACCTGACCCAGTGAATAATCTATACATATCTGAGGCAATCAAACAGATGGAATATGAAAACTGGAAGGTCCAGTTCTTAAACATCACTTATCTAACAGAGTTCAGGTATGATGGTCACCCTTCAAAGTATCGTGAACCAGGGACTCCAGCGGATGCTCCACAAGACTGCAGCCACTGGTGCCTACCTGGAGTGCCTGACACATGGAATCAACTTATTTATGGAAATCTACAGGCAAAAGGATTCAGAACCAAGTAA

Protein Analysis

392

Amino Acids

45.48

Weight (kDa)

6.89

Isoelectric Point (pI)

47.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 76 - 129 2.2e-18 PMR5 N terminal Domain
PC-Esterase PF13839 132 - 284 1.2e-45 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
PC-Esterase PF13839 284 - 381 7.9e-27 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11570 AT5G06230 AT5G06230 AT5G06230
fragaria_vesca FvH4_6g19550 FvH4_6g19550 FvH4_6g19550 FvH4_6g19550
malus_domestica MD12G1031900.v1.1 MD14G1032200.v1.1
prunus_persica Prupe.7G100700_v2.0.a1
pyrus_communis pycom12g02960
rosa_chinensis RchiOBHm_Chr3g0474891
rosa_laevigata RLG00000023883
rosa_multiflora Rmu_sc0008866.1_g000002
rosa_roxburghii Rroxscaffold_6G00406760
rosa_rugosa Rorug03G0145200
rosa_samantha Rh3AG194800 Rh3BG224700 Rh3CG219700 Rh3DG220300
rosa_wichuraiana Rw3G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 619, 642, 1101
AccB7I CCANNNNNTGG 1 cut(s) 717
AccBSI CCGCTC 1 cut(s) 85
AccI GTMKAC 1 cut(s) 690
AciI CCGC 4 cut(s) 83, 377, 873, 1073
AclWI GGATC 3 cut(s) 12, 302, 683
AcsI RAATTY 1 cut(s) 817
AcuI CTGAAG 1 cut(s) 260
AfaI GTAC 1 cut(s) 284
AfiI CCNNNNNNNGG 2 cut(s) 318, 717
AgsI TTSAA 4 cut(s) 11, 608, 923, 1047
AjnI CCWGG 3 cut(s) 585, 1060, 1108
AloI GAACNNNNNNTCC 2 cut(s) 717, 749
Alw26I GTCTC 3 cut(s) 63, 252, 900
AlwI GGATC 3 cut(s) 12, 302, 683
AlwNI CAGNNNCTG 1 cut(s) 1098
ApeKI GCWGC 2 cut(s) 324, 1092
ApoI RAATTY 1 cut(s) 817
AspS9I GGNCC 2 cut(s) 253, 991
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 2 cut(s) 127, 1031
AvaII GGWCC 2 cut(s) 253, 991
BaeGI GKGCMC 3 cut(s) 622, 645, 756
BanI GGYRCC 3 cut(s) 619, 642, 1101
BbsI GAAGAC 1 cut(s) 94
BbvI GCAGC 2 cut(s) 336, 1104
BccI CCATC 7 cut(s) 122, 243, 360, 778, 829, 964, 1028
BciT130I CCWGG 3 cut(s) 587, 1062, 1110
BciVI GTATCC 1 cut(s) 520
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 3 cut(s) 63, 252, 900
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 2 cut(s) 1090, 1151
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 2 cut(s) 325, 1093
BlsI GCNGC 2 cut(s) 326, 1094
Bme1390I CCNGG 4 cut(s) 312, 587, 1062, 1110
Bme18I GGWCC 2 cut(s) 253, 991
BmgT120I GGNCC 2 cut(s) 253, 991
BmiI GGNNCC 8 cut(s) 172, 254, 470, 545, 621, 644, 897, 1103
BmrFI CCNGG 4 cut(s) 312, 587, 1062, 1110
BmrI ACTGGG 1 cut(s) 926
BmsI GCATC 2 cut(s) 668, 1066
BmuI ACTGGG 1 cut(s) 926
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 2 cut(s) 1053, 1131
BpuMI CCSGG 1 cut(s) 312
BsaI GGTCTC 2 cut(s) 63, 900
BsaJI CCNNGG 2 cut(s) 37, 1061
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 717
Bse118I RCCGGY 2 cut(s) 373, 806
Bse1I ACTGG 7 cut(s) 472, 707, 761, 932, 989, 994, 1103
BseBI CCWGG 3 cut(s) 587, 1062, 1110
BseDI CCNNGG 2 cut(s) 37, 1061
BseGI GGATG 4 cut(s) 215, 789, 835, 1081
BseLI CCNNNNNNNGG 2 cut(s) 318, 717
BseMII CTCAG 2 cut(s) 41, 945
BseNI ACTGG 7 cut(s) 472, 707, 761, 932, 989, 994, 1103
BseRI GAGGAG 1 cut(s) 782
BseSI GKGCMC 3 cut(s) 622, 645, 756
BseXI GCAGC 2 cut(s) 336, 1104
BsgI GTGCAG 2 cut(s) 343, 768
Bsh1285I CGRYCG 1 cut(s) 462
BshNI GGYRCC 3 cut(s) 619, 642, 1101
BsiEI CGRYCG 1 cut(s) 462
BsiSI CCGG 3 cut(s) 312, 374, 807
BslFI GGGAC 1 cut(s) 1078
BslI CCNNNNNNNGG 2 cut(s) 318, 717
BsmAI GTCTC 3 cut(s) 63, 252, 900
BsmFI GGGAC 1 cut(s) 1078
BsmI GAATGC 1 cut(s) 842
Bso31I GGTCTC 2 cut(s) 63, 900
Bsp1286I GDGCHC 3 cut(s) 622, 645, 756
Bsp1407I TGTACA 1 cut(s) 282
Bsp143I GATC 5 cut(s) 4, 307, 385, 459, 675
BspACI CCGC 4 cut(s) 83, 377, 873, 1073
BspCNI CTCAG 2 cut(s) 40, 946
BspLI GGNNCC 8 cut(s) 172, 254, 470, 545, 621, 644, 897, 1103
BspMAI CTGCAG 1 cut(s) 1094
BspPI GGATC 3 cut(s) 12, 302, 683
BspT107I GGYRCC 3 cut(s) 619, 642, 1101
BspTNI GGTCTC 2 cut(s) 63, 900
BsrBI CCGCTC 1 cut(s) 85
BsrFI RCCGGY 2 cut(s) 373, 806
BsrGI TGTACA 1 cut(s) 282
BsrI ACTGG 7 cut(s) 472, 707, 761, 932, 989, 994, 1103
BssAI RCCGGY 2 cut(s) 373, 806
BssECI CCNNGG 2 cut(s) 37, 1061
BssMI GATC 5 cut(s) 4, 307, 385, 459, 675
BssT1I CCWWGG 1 cut(s) 37
Bst2UI CCWGG 3 cut(s) 587, 1062, 1110
Bst4CI ACNGT 3 cut(s) 604, 682, 778
Bst6I CTCTTC 1 cut(s) 61
BstAUI TGTACA 1 cut(s) 282
BstC8I GCNNGC 6 cut(s) 379, 406, 451, 494, 498, 875
BstDEI CTNAG 2 cut(s) 27, 954
BstEII GGTNACC 1 cut(s) 1037
BstF5I GGATG 4 cut(s) 215, 789, 835, 1081
BstKTI GATC 5 cut(s) 7, 310, 388, 462, 678
BstMAI GTCTC 3 cut(s) 63, 252, 900
BstMBI GATC 5 cut(s) 4, 307, 385, 459, 675
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 4 cut(s) 294, 321, 493, 1101
BstNI CCWGG 3 cut(s) 587, 1062, 1110
BstNSI RCATGY 2 cut(s) 155, 496
BstPI GGTNACC 1 cut(s) 1037
BstSCI CCNGG 4 cut(s) 310, 585, 1060, 1108
BstSFI CTRYAG 2 cut(s) 1090, 1151
BstSLI GKGCMC 3 cut(s) 622, 645, 756
BstV1I GCAGC 2 cut(s) 336, 1104
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 520
BtgZI GCGATG 2 cut(s) 100, 379
BtsCI GGATG 4 cut(s) 215, 789, 835, 1081
BtsIMutI CAGTG 6 cut(s) 479, 700, 751, 754, 939, 1096
Cac8I GCNNGC 6 cut(s) 379, 406, 451, 494, 498, 875
CaiI CAGNNNCTG 1 cut(s) 1098
Cfr10I RCCGGY 2 cut(s) 373, 806
Cfr13I GGNCC 2 cut(s) 253, 991
Csp6I GTAC 1 cut(s) 283
CviAII CATG 5 cut(s) 152, 269, 493, 861, 1125
CviJI RGCY 9 cut(s) 34, 315, 327, 381, 666, 806, 877, 898, 1095
CviKI_1 RGCY 9 cut(s) 34, 315, 327, 381, 666, 806, 877, 898, 1095
CviQI GTAC 1 cut(s) 283
DdeI CTNAG 2 cut(s) 27, 954
DpnI GATC 5 cut(s) 6, 309, 387, 461, 677
DpnII GATC 5 cut(s) 4, 307, 385, 459, 675
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 37
Eco31I GGTCTC 2 cut(s) 63, 900
Eco47I GGWCC 2 cut(s) 253, 991
Eco57I CTGAAG 1 cut(s) 260
Eco91I GGTNACC 1 cut(s) 1037
EcoO65I GGTNACC 1 cut(s) 1037
EcoRII CCWGG 3 cut(s) 585, 1060, 1108
EcoT14I CCWWGG 1 cut(s) 37
EcoT22I ATGCAT 1 cut(s) 661
ErhI CCWWGG 1 cut(s) 37
FaeI CATG 5 cut(s) 155, 272, 496, 864, 1128
FaqI GGGAC 1 cut(s) 1078
FatI CATG 5 cut(s) 151, 268, 492, 860, 1124
FauI CCCGC 2 cut(s) 370, 866
FblI GTMKAC 1 cut(s) 690
Fnu4HI GCNGC 2 cut(s) 325, 1093
FokI GGATG 4 cut(s) 202, 796, 842, 1088
Fsp4HI GCNGC 2 cut(s) 325, 1093
FspBI CTAG 1 cut(s) 522
GluI GCNGC 2 cut(s) 325, 1093
GsuI CTGGAG 2 cut(s) 1053, 1131
HapII CCGG 3 cut(s) 312, 374, 807
Hin1II CATG 5 cut(s) 155, 272, 496, 864, 1128
HincII GTYRAC 1 cut(s) 691
HindII GTYRAC 1 cut(s) 691
HinfI GANTC 9 cut(s) 146, 265, 455, 479, 525, 582, 1066, 1129, 1164
HpaII CCGG 3 cut(s) 312, 374, 807
HphI GGTGA 2 cut(s) 127, 1031
Hpy166II GTNNAC 2 cut(s) 691, 1058
Hpy188I TCNGA 7 cut(s) 30, 257, 339, 464, 738, 955, 1169
Hpy188III TCNNGA 6 cut(s) 305, 522, 673, 847, 920, 1055
Hpy8I GTNNAC 2 cut(s) 691, 1058
HpyAV CCTTC 6 cut(s) 83, 459, 634, 863, 982, 1053
HpyCH4III ACNGT 3 cut(s) 604, 682, 778
HpyCH4V TGCA 7 cut(s) 272, 324, 492, 500, 659, 749, 1092
HpyF10VI GCNNNNNNNGC 4 cut(s) 294, 321, 493, 1101
HpyF3I CTNAG 2 cut(s) 27, 954
Hsp92II CATG 5 cut(s) 155, 272, 496, 864, 1128
Kzo9I GATC 5 cut(s) 4, 307, 385, 459, 675
LmnI GCTCC 3 cut(s) 725, 895, 1084
Lsp1109I GCAGC 2 cut(s) 336, 1104
LweI GCATC 2 cut(s) 668, 1066
MaeI CTAG 1 cut(s) 522
MaeIII GTNAC 3 cut(s) 230, 788, 1037
MalI GATC 5 cut(s) 6, 309, 387, 461, 677
MbiI CCGCTC 1 cut(s) 85
MboI GATC 5 cut(s) 4, 307, 385, 459, 675
MboII GAAGA 4 cut(s) 78, 94, 258, 359
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 622, 645, 756
MluCI AATT 2 cut(s) 695, 817
MlyI GAGTC 3 cut(s) 140, 449, 1060
MmeI TCCRAC 3 cut(s) 317, 537, 703
MnlI CCTC 9 cut(s) 25, 229, 387, 557, 608, 760, 793, 843, 949
Mph1103I ATGCAT 1 cut(s) 661
MseI TTAA 3 cut(s) 399, 684, 1001
MspA1I CMGCKG 1 cut(s) 1073
MspI CCGG 3 cut(s) 312, 374, 807
MspR9I CCNGG 4 cut(s) 312, 587, 1062, 1110
Mva1269I GAATGC 1 cut(s) 842
MvaI CCWGG 3 cut(s) 587, 1062, 1110
MwoI GCNNNNNNNGC 4 cut(s) 294, 321, 493, 1101
NciI CCSGG 1 cut(s) 312
NdeII GATC 5 cut(s) 4, 307, 385, 459, 675
NlaIII CATG 5 cut(s) 155, 272, 496, 864, 1128
NlaIV GGNNCC 8 cut(s) 172, 254, 470, 545, 621, 644, 897, 1103
NmuCI GTSAC 3 cut(s) 230, 788, 1037
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 2 cut(s) 155, 496
PaeI GCATGC 1 cut(s) 496
PcsI WCGNNNNNNNCGW 3 cut(s) 102, 361, 433
PctI GAATGC 1 cut(s) 842
PfeI GAWTC 6 cut(s) 265, 479, 525, 582, 1129, 1164
PflMI CCANNNNNTGG 1 cut(s) 717
PfoI TCCNGGA 1 cut(s) 585
PkrI GCNGC 2 cut(s) 326, 1094
Ple19I CGATCG 1 cut(s) 462
PleI GAGTC 3 cut(s) 140, 449, 1060
PpsI GAGTC 3 cut(s) 140, 449, 1060
Psp6I CCWGG 3 cut(s) 585, 1060, 1108
PspEI GGTNACC 1 cut(s) 1037
PspGI CCWGG 3 cut(s) 585, 1060, 1108
PspN4I GGNNCC 8 cut(s) 172, 254, 470, 545, 621, 644, 897, 1103
PspPI GGNCC 2 cut(s) 253, 991
PstI CTGCAG 1 cut(s) 1094
PstNI CAGNNNCTG 1 cut(s) 1098
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 462
RsaI GTAC 1 cut(s) 284
RsaNI GTAC 1 cut(s) 283
SalI GTCGAC 1 cut(s) 689
SaqAI TTAA 3 cut(s) 399, 684, 1001
SatI GCNGC 2 cut(s) 325, 1093
Sau3AI GATC 5 cut(s) 4, 307, 385, 459, 675
Sau96I GGNCC 2 cut(s) 253, 991
SchI GAGTC 3 cut(s) 140, 449, 1060
ScrFI CCNGG 4 cut(s) 312, 587, 1062, 1110
SduI GDGCHC 3 cut(s) 622, 645, 756
SfaNI GCATC 2 cut(s) 668, 1066
SfcI CTRYAG 2 cut(s) 1090, 1151
SinI GGWCC 2 cut(s) 253, 991
SphI GCATGC 1 cut(s) 496
Sse9I AATT 2 cut(s) 695, 817
SsiI CCGC 4 cut(s) 83, 377, 873, 1073
SspI AATATT 1 cut(s) 611
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 4 cut(s) 310, 585, 1060, 1108
StyI CCWWGG 1 cut(s) 37
TaaI ACNGT 3 cut(s) 604, 682, 778
TaqI TCGA 5 cut(s) 306, 427, 458, 690, 798
TasI AATT 2 cut(s) 695, 817
TatI WGTACW 1 cut(s) 282
TfiI GAWTC 6 cut(s) 265, 479, 525, 582, 1129, 1164
Tru1I TTAA 3 cut(s) 399, 684, 1001
Tru9I TTAA 3 cut(s) 399, 684, 1001
TscAI CASTG 6 cut(s) 479, 707, 751, 761, 939, 1103
TseFI GTSAC 3 cut(s) 230, 788, 1037
TseI GCWGC 2 cut(s) 324, 1092
Tsp45I GTSAC 3 cut(s) 230, 788, 1037
TspDTI ATGAA 3 cut(s) 546, 882, 993
TspGWI ACGGA 1 cut(s) 908
TspRI CASTG 6 cut(s) 479, 707, 751, 761, 939, 1103
Van91I CCANNNNNTGG 1 cut(s) 717
VpaK11BI GGWCC 2 cut(s) 253, 991
XapI RAATTY 1 cut(s) 817
XbaI TCTAGA 1 cut(s) 521
XceI RCATGY 2 cut(s) 155, 496
XmiI GTMKAC 1 cut(s) 690
XspI CTAG 1 cut(s) 522
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.