pycom12g02960

Protein trichome birefringence-like 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009484
Physical Location & Seq
Reverse (-)
2661682 .. 2663865
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02960.4

Sequence Viewer

Length: 1218 bp
ATGGATCTCCAACAATCTCATCAACCTCAGAAGCTCCAAAATTTCCCCTTTTCATTTCTAATGAGAACAGAACTTCGTTACGCAATCTCTTTCTTCCTTCTCCTAGTTTCTTCCTTGTTTATTCTCAGTCTCGTAACCCCATTCGACTCTCAGTTTCTATCCGATCATTTGGGTTTCTTATCCCAAATCCTACCAAAAAATAATCACAAGTTGCCTCCAGGAACTTGTGATTATTCCTACGGCAGATGGGTCCGCGACAAGACTCATCCGCTTCAGTCCTACACCGAGAACTGCCCGTTTCTCGACCCTGGCTTCAGGTGCAGCCAGAGTGGTCGAAAAGATGAAGGTTATCGGAGATGGCGGTGGCAACCGGCTGGCTGTGATGTTTTGAGATTTAACGCAAGCGACTTCTTGGAAAGAAGCAGAAACGGACGGATAGTGTTTGCCGGCGACTCCATCGGAAGGAACCAGTGGGAATCCATGCTATGCATGCTTGCAGAAGGGGCGTCCAACAAGTCCAGAATCTATGAAGTGAATGGGAACCCCATAACAAAACACAAGGGTTTTCTGTCGATGCGGTTCCAGGACTACAACCTCACAGTTGAATATTATAGGGCACCCTTCCTTGTGGTTGTAGGACACCCACCCAAAAATTCACCAAACCAGGTCAGGACCACAGTTAAGCTCGATCAATTCCATTGGTACTCCAAACACTGGGTTGGAGCAGATGTTCTAGTTTTCAGTGCAGGGCACTGGTGGAACGAAGGCAAAACAGCAAAGATGGGCTGCTACTTTGAGGAAGACGGCAAAGTGAATTTGACAATGGGAGCGATGGAAGCATTCAGGAGGTCTATGCAAACCTGGAAATTTTGGGTGCTCAAGAATTTAGACCCTCGAAGGACTCGGGTTTTCTTCCGAAGCTTCTCTCCGGTGCATTACAGGAACGGCACATGGAATGAAGGTTGTGGGTGTGATACCTATTCGGAGCCAGAAACAGACTACAAAAATCTTGCAGCTGACCCAGTGAATAATCTCTACCTTTCTGAATCAATCAAACAGTTGGAATACGAAAACTGGAAGGTCCAGTTCTTAAACATCACGTATCTAACAGACTTCAGGAAGGATGGTCACCCCTCAAAGTATCGTGAGCCAGGAACTCCGGCTGATGCTCCACAAGACTGCAGCCACTGGTGCTTACCCGGTGTACCTCAGATTTGA

Protein Analysis

406

Amino Acids

47.21

Weight (kDa)

8.78

Isoelectric Point (pI)

49.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11570 AT5G06230 AT5G06230 AT5G06230
fragaria_vesca FvH4_6g19550 FvH4_6g19550 FvH4_6g19550 FvH4_6g19550
malus_domestica MD12G1031900.v1.1 MD14G1032200.v1.1
prunus_persica Prupe.7G100700_v2.0.a1
pyrus_communis pycom12g02960
rosa_chinensis RchiOBHm_Chr3g0474891
rosa_laevigata RLG00000023883
rosa_multiflora Rmu_sc0008866.1_g000002
rosa_roxburghii Rroxscaffold_6G00406760
rosa_rugosa Rorug03G0145200
rosa_samantha Rh3AG194800 Rh3BG224700 Rh3CG219700 Rh3DG220300
rosa_wichuraiana Rw3G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 616
AccB7I CCANNNNNTGG 1 cut(s) 714
AccII CGCG 1 cut(s) 255
AciI CCGC 4 cut(s) 253, 269, 361, 577
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 5 cut(s) 40, 652, 814, 866, 883
AcuI CTGAAG 3 cut(s) 257, 298, 1099
AcyI GRCGYC 1 cut(s) 506
AfaI GTAC 2 cut(s) 704, 1206
AfiI CCNNNNNNNGG 2 cut(s) 462, 714
AgsI TTSAA 1 cut(s) 605
AjnI CCWGG 6 cut(s) 217, 307, 582, 663, 860, 1150
AloI GAACNNNNNNTCC 2 cut(s) 714, 746
AluBI AGCT 4 cut(s) 34, 685, 921, 1016
AluI AGCT 4 cut(s) 34, 685, 921, 1016
Alw21I GWGCWC 1 cut(s) 879
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 1188
Ama87I CYCGRG 1 cut(s) 903
ApeKI GCWGC 4 cut(s) 321, 786, 1013, 1182
ApoI RAATTY 5 cut(s) 40, 652, 814, 866, 883
AspS9I GGNCC 3 cut(s) 250, 672, 1081
AsuC2I CCSGG 1 cut(s) 1200
AsuHPI GGTGA 2 cut(s) 648, 1121
AvaI CYCGRG 1 cut(s) 903
AvaII GGWCC 3 cut(s) 250, 672, 1081
BaeGI GKGCMC 2 cut(s) 619, 753
BanI GGYRCC 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 807
Bbv12I GWGCWC 1 cut(s) 879
BbvI GCAGC 4 cut(s) 333, 773, 1025, 1194
BccI CCATC 6 cut(s) 240, 351, 464, 775, 826, 1118
BceAI ACGGC 3 cut(s) 256, 820, 961
BciT130I CCWGG 6 cut(s) 219, 309, 584, 665, 862, 1152
BcnI CCSGG 1 cut(s) 1200
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 2 cut(s) 104, 734
BfmI CTRYAG 1 cut(s) 1180
BisI GCNGC 4 cut(s) 322, 787, 1014, 1183
BlsI GCNGC 4 cut(s) 323, 788, 1015, 1184
Bme1390I CCNGG 7 cut(s) 219, 309, 584, 665, 862, 1152, 1200
Bme18I GGWCC 3 cut(s) 250, 672, 1081
BmeT110I CYCGRG 1 cut(s) 903
BmgT120I GGNCC 3 cut(s) 250, 672, 1081
BmiI GGNNCC 6 cut(s) 251, 467, 542, 581, 618, 987
BmrFI CCNGG 7 cut(s) 219, 309, 584, 665, 862, 1152, 1200
BmrI ACTGGG 2 cut(s) 724, 1016
BmsI GCATC 2 cut(s) 564, 1156
BmuI ACTGGG 2 cut(s) 724, 1016
BpiI GAAGAC 1 cut(s) 807
BpmI CTGGAG 1 cut(s) 201
BpuEI CTTGAG 1 cut(s) 863
BpuMI CCSGG 1 cut(s) 1200
BsaAI YACGTR 1 cut(s) 1101
BsaHI GRCGYC 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 307
BsaWI WCCGGW 1 cut(s) 928
BsaXI ACNNNNNCTCC 2 cut(s) 714, 744
Bsc4I CCNNNNNNNGG 2 cut(s) 462, 714
Bse118I RCCGGY 2 cut(s) 370, 446
Bse1I ACTGG 7 cut(s) 469, 719, 758, 1022, 1079, 1084, 1193
BseBI CCWGG 6 cut(s) 219, 309, 584, 665, 862, 1152
BseDI CCNNGG 1 cut(s) 307
BseGI GGATG 2 cut(s) 265, 1129
BseLI CCNNNNNNNGG 2 cut(s) 462, 714
BseMII CTCAG 3 cut(s) 41, 139, 164
BseNI ACTGG 7 cut(s) 469, 719, 758, 1022, 1079, 1084, 1193
BseSI GKGCMC 2 cut(s) 619, 753
BseXI GCAGC 4 cut(s) 333, 773, 1025, 1194
BsgI GTGCAG 2 cut(s) 340, 765
Bsh1236I CGCG 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 879
BsiHKCI CYCGRG 1 cut(s) 903
BsiSI CCGG 5 cut(s) 371, 447, 929, 1160, 1200
BslI CCNNNNNNNGG 2 cut(s) 462, 714
BsmAI GTCTC 1 cut(s) 134
BsmI GAATGC 1 cut(s) 839
BsoBI CYCGRG 1 cut(s) 903
Bsp1286I GDGCHC 3 cut(s) 619, 753, 879
Bsp143I GATC 3 cut(s) 4, 163, 688
BspACI CCGC 4 cut(s) 253, 269, 361, 577
BspCNI CTCAG 3 cut(s) 40, 138, 163
BspFNI CGCG 1 cut(s) 255
BspLI GGNNCC 6 cut(s) 251, 467, 542, 581, 618, 987
BspMAI CTGCAG 1 cut(s) 1184
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 616
BsrFI RCCGGY 2 cut(s) 370, 446
BsrI ACTGG 7 cut(s) 469, 719, 758, 1022, 1079, 1084, 1193
BssAI RCCGGY 2 cut(s) 370, 446
BssECI CCNNGG 1 cut(s) 307
BssMI GATC 3 cut(s) 4, 163, 688
BssNI GRCGYC 1 cut(s) 506
Bst2UI CCWGG 6 cut(s) 219, 309, 584, 665, 862, 1152
Bst4CI ACNGT 3 cut(s) 601, 679, 1059
BstACI GRCGYC 1 cut(s) 506
BstBAI YACGTR 1 cut(s) 1101
BstC8I GCNNGC 5 cut(s) 376, 403, 448, 491, 495
BstDEI CTNAG 4 cut(s) 27, 125, 150, 1209
BstEII GGTNACC 1 cut(s) 1127
BstF5I GGATG 2 cut(s) 265, 1129
BstFNI CGCG 1 cut(s) 255
BstKTI GATC 3 cut(s) 7, 166, 691
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 4, 163, 688
BstMWI GCNNNNNNNGC 5 cut(s) 318, 490, 503, 836, 1191
BstNI CCWGG 6 cut(s) 219, 309, 584, 665, 862, 1152
BstNSI RCATGY 1 cut(s) 493
BstPI GGTNACC 1 cut(s) 1127
BstSCI CCNGG 7 cut(s) 217, 307, 582, 663, 860, 1150, 1198
BstSFI CTRYAG 1 cut(s) 1180
BstSLI GKGCMC 2 cut(s) 619, 753
BstUI CGCG 1 cut(s) 255
BstV1I GCAGC 4 cut(s) 333, 773, 1025, 1194
BstV2I GAAGAC 1 cut(s) 807
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtgZI GCGATG 1 cut(s) 845
BtsCI GGATG 2 cut(s) 265, 1129
BtsIMutI CAGTG 6 cut(s) 476, 712, 748, 751, 1029, 1186
Cac8I GCNNGC 5 cut(s) 376, 403, 448, 491, 495
CaiI CAGNNNCTG 1 cut(s) 1188
Cfr10I RCCGGY 2 cut(s) 370, 446
Cfr13I GGNCC 3 cut(s) 250, 672, 1081
CseI GACGC 1 cut(s) 495
CsiI ACCWGGT 1 cut(s) 663
Csp6I GTAC 2 cut(s) 703, 1205
CviAII CATG 3 cut(s) 481, 490, 951
CviQI GTAC 2 cut(s) 703, 1205
DdeI CTNAG 4 cut(s) 27, 125, 150, 1209
DpnI GATC 3 cut(s) 6, 165, 690
DpnII GATC 3 cut(s) 4, 163, 688
Eco47I GGWCC 3 cut(s) 250, 672, 1081
Eco57I CTGAAG 3 cut(s) 257, 298, 1099
Eco88I CYCGRG 1 cut(s) 903
Eco91I GGTNACC 1 cut(s) 1127
EcoO65I GGTNACC 1 cut(s) 1127
EcoRII CCWGG 6 cut(s) 217, 307, 582, 663, 860, 1150
EcoT22I ATGCAT 1 cut(s) 491
FaeI CATG 3 cut(s) 484, 493, 954
FaiI YATR 8 cut(s) 482, 487, 491, 528, 548, 612, 854, 952
FatI CATG 3 cut(s) 480, 489, 950
Fnu4HI GCNGC 4 cut(s) 322, 787, 1014, 1183
FokI GGATG 2 cut(s) 252, 1136
Fsp4HI GCNGC 4 cut(s) 322, 787, 1014, 1183
FspBI CTAG 2 cut(s) 104, 734
GluI GCNGC 4 cut(s) 322, 787, 1014, 1183
GsuI CTGGAG 1 cut(s) 201
HapII CCGG 5 cut(s) 371, 447, 929, 1160, 1200
HgaI GACGC 1 cut(s) 495
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 3 cut(s) 484, 493, 954
HindIII AAGCTT 1 cut(s) 919
HinfI GANTC 7 cut(s) 146, 262, 452, 476, 522, 901, 1046
HpaII CCGG 5 cut(s) 371, 447, 929, 1160, 1200
HphI GGTGA 2 cut(s) 648, 1121
Hpy166II GTNNAC 1 cut(s) 1205
Hpy188I TCNGA 8 cut(s) 30, 163, 354, 461, 917, 985, 1045, 1212
Hpy188III TCNNGA 7 cut(s) 302, 519, 670, 844, 880, 1117, 1145
Hpy8I GTNNAC 1 cut(s) 1205
HpyCH4III ACNGT 3 cut(s) 601, 679, 1059
HpyCH4IV ACGT 1 cut(s) 1100
HpyCH4V TGCA 8 cut(s) 321, 489, 497, 746, 856, 934, 1013, 1182
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 490, 503, 836, 1191
HpyF3I CTNAG 4 cut(s) 27, 125, 150, 1209
HpySE526I ACGT 1 cut(s) 1100
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 3 cut(s) 484, 493, 954
KroI GCCGGC 1 cut(s) 446
KroNI GCCGGC 1 cut(s) 448
Kzo9I GATC 3 cut(s) 4, 163, 688
LmnI GCTCC 5 cut(s) 39, 722, 827, 985, 1174
Lsp1109I GCAGC 4 cut(s) 333, 773, 1025, 1194
LweI GCATC 2 cut(s) 564, 1156
MabI ACCWGGT 1 cut(s) 663
MaeI CTAG 2 cut(s) 104, 734
MaeII ACGT 1 cut(s) 1100
MaeIII GTNAC 3 cut(s) 77, 133, 1127
MalI GATC 3 cut(s) 6, 165, 690
MboI GATC 3 cut(s) 4, 163, 688
MboII GAAGA 4 cut(s) 85, 102, 812, 904
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 619, 753, 879
MluCI AATT 6 cut(s) 40, 652, 692, 814, 866, 883
MlyI GAGTC 4 cut(s) 140, 256, 446, 895
MmeI TCCRAC 4 cut(s) 34, 534, 700, 1041
MnlI CCTC 8 cut(s) 36, 225, 605, 790, 840, 903, 1144, 1218
Mph1103I ATGCAT 1 cut(s) 491
MroNI GCCGGC 1 cut(s) 446
MseI TTAA 3 cut(s) 396, 681, 1091
MspA1I CMGCKG 1 cut(s) 1016
MspI CCGG 5 cut(s) 371, 447, 929, 1160, 1200
MspR9I CCNGG 7 cut(s) 219, 309, 584, 665, 862, 1152, 1200
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 6 cut(s) 219, 309, 584, 665, 862, 1152
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 5 cut(s) 318, 490, 503, 836, 1191
NaeI GCCGGC 1 cut(s) 448
NciI CCSGG 1 cut(s) 1200
NdeII GATC 3 cut(s) 4, 163, 688
NgoMIV GCCGGC 1 cut(s) 446
NlaIII CATG 3 cut(s) 484, 493, 954
NlaIV GGNNCC 6 cut(s) 251, 467, 542, 581, 618, 987
NmuCI GTSAC 1 cut(s) 1127
NsiI ATGCAT 1 cut(s) 491
NspI RCATGY 1 cut(s) 493
PaeI GCATGC 1 cut(s) 493
PctI GAATGC 1 cut(s) 839
PdiI GCCGGC 1 cut(s) 448
PfeI GAWTC 3 cut(s) 476, 522, 1046
PflMI CCANNNNNTGG 1 cut(s) 714
PfoI TCCNGGA 2 cut(s) 217, 582
PkrI GCNGC 4 cut(s) 323, 788, 1015, 1184
PleI GAGTC 4 cut(s) 140, 256, 446, 895
PpsI GAGTC 4 cut(s) 140, 256, 446, 895
Ppu21I YACGTR 1 cut(s) 1101
Psp6I CCWGG 6 cut(s) 217, 307, 582, 663, 860, 1150
PspEI GGTNACC 1 cut(s) 1127
PspGI CCWGG 6 cut(s) 217, 307, 582, 663, 860, 1150
PspN4I GGNNCC 6 cut(s) 251, 467, 542, 581, 618, 987
PspPI GGNCC 3 cut(s) 250, 672, 1081
PstI CTGCAG 1 cut(s) 1184
PstNI CAGNNNCTG 1 cut(s) 1188
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 1016
RsaI GTAC 2 cut(s) 704, 1206
RsaNI GTAC 2 cut(s) 703, 1205
SaqAI TTAA 3 cut(s) 396, 681, 1091
SatI GCNGC 4 cut(s) 322, 787, 1014, 1183
Sau3AI GATC 3 cut(s) 4, 163, 688
Sau96I GGNCC 3 cut(s) 250, 672, 1081
SchI GAGTC 4 cut(s) 140, 256, 446, 895
ScrFI CCNGG 7 cut(s) 219, 309, 584, 665, 862, 1152, 1200
SduI GDGCHC 3 cut(s) 619, 753, 879
SexAI ACCWGGT 1 cut(s) 663
SfaNI GCATC 2 cut(s) 564, 1156
SfcI CTRYAG 1 cut(s) 1180
SinI GGWCC 3 cut(s) 250, 672, 1081
SmlI CTYRAG 1 cut(s) 878
SmoI CTYRAG 1 cut(s) 878
SphI GCATGC 1 cut(s) 493
Sse9I AATT 6 cut(s) 40, 652, 692, 814, 866, 883
SsiI CCGC 4 cut(s) 253, 269, 361, 577
SspI AATATT 1 cut(s) 608
SspMI CTAG 2 cut(s) 104, 734
StyD4I CCNGG 7 cut(s) 217, 307, 582, 663, 860, 1150, 1198
TaaI ACNGT 3 cut(s) 601, 679, 1059
TaiI ACGT 1 cut(s) 1103
TaqI TCGA 6 cut(s) 144, 303, 334, 572, 687, 895
TasI AATT 6 cut(s) 40, 652, 692, 814, 866, 883
TfiI GAWTC 3 cut(s) 476, 522, 1046
Tru1I TTAA 3 cut(s) 396, 681, 1091
Tru9I TTAA 3 cut(s) 396, 681, 1091
TscAI CASTG 6 cut(s) 476, 719, 748, 758, 1029, 1193
TseFI GTSAC 1 cut(s) 1127
TseI GCWGC 4 cut(s) 321, 786, 1013, 1182
Tsp45I GTSAC 1 cut(s) 1127
TspDTI ATGAA 4 cut(s) 42, 357, 543, 972
TspGWI ACGGA 2 cut(s) 444, 448
TspRI CASTG 6 cut(s) 476, 719, 748, 758, 1029, 1193
Van91I CCANNNNNTGG 1 cut(s) 714
VpaK11BI GGWCC 3 cut(s) 250, 672, 1081
XapI RAATTY 5 cut(s) 40, 652, 814, 866, 883
XceI RCATGY 1 cut(s) 493
XspI CTAG 2 cut(s) 104, 734
Zsp2I ATGCAT 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.