Rh3BG224700

Protein trichome birefringence-like 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0009484
Physical Location & Seq
Reverse (-)
20515712 .. 20516152
441 bp
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UTR
Exon/CDS
Intron
Rh3BG224700.1

Sequence Viewer

Length: 441 bp
ATGGACTTTTGTGATCACAGGTTTAACGCAAGCGACTTCTTGGAGAGAAGTCGAAATGGACGAATAGTGTTTGCTGGCGACTCGATCGGAAGGAACCAGTGGGAATCTTTGCTGTGCATGCTTGCAGAAGCAGTATCCAACAAGTCTAGAATCTATGAAGTAAATGGGAACCCCATTACAAAACACACAGGTTTTCTATCAATGCGATTCCAGGACTACAACCTCACAGTTGAATATTACAGGGCACCCTTCCTTGTGGTCGTAGGGCACCCAACACAAAATGCATCAAGCCAAGTCAGGATCACAGTTAAGGTCGACCAATTCCACTGGTTTTCCAAACATTGGGTTGGAGCAGATGTTCTGATTTTCAGTGCAGGGCACTGGTGGAACGAGGATAAAACAGTAAGGATGTATGACCATTCCTTCCCTATTGTACGATGA

Protein Analysis

146

Amino Acids

17.06

Weight (kDa)

7.87

Isoelectric Point (pI)

22.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 7 - 135 4e-42 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11570 AT5G06230 AT5G06230 AT5G06230
fragaria_vesca FvH4_6g19550 FvH4_6g19550 FvH4_6g19550 FvH4_6g19550
malus_domestica MD12G1031900.v1.1 MD14G1032200.v1.1
prunus_persica Prupe.7G100700_v2.0.a1
pyrus_communis pycom12g02960
rosa_chinensis RchiOBHm_Chr3g0474891
rosa_laevigata RLG00000023883
rosa_multiflora Rmu_sc0008866.1_g000002
rosa_roxburghii Rroxscaffold_6G00406760
rosa_rugosa Rorug03G0145200
rosa_samantha Rh3AG194800 Rh3BG224700 Rh3CG219700 Rh3DG220300
rosa_wichuraiana Rw3G017730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 244, 267
AccB7I CCANNNNNTGG 1 cut(s) 342
AccI GTMKAC 1 cut(s) 315
AclWI GGATC 1 cut(s) 308
AfaI GTAC 1 cut(s) 435
AfiI CCNNNNNNNGG 1 cut(s) 342
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 1 cut(s) 210
AloI GAACNNNNNNTCC 2 cut(s) 342, 374
AlwI GGATC 1 cut(s) 308
BaeGI GKGCMC 3 cut(s) 247, 270, 381
BanI GGYRCC 2 cut(s) 244, 267
BciT130I CCWGG 1 cut(s) 212
BciVI GTATCC 1 cut(s) 145
BclI TGATCA 1 cut(s) 13
BfaI CTAG 1 cut(s) 147
BfuI GTATCC 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 212
BmiI GGNNCC 4 cut(s) 95, 170, 246, 269
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 1 cut(s) 293
BsaXI ACNNNNNCTCC 2 cut(s) 342, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 342
Bse1I ACTGG 3 cut(s) 97, 332, 386
BseBI CCWGG 1 cut(s) 212
BseGI GGATG 1 cut(s) 414
BseLI CCNNNNNNNGG 1 cut(s) 342
BseNI ACTGG 3 cut(s) 97, 332, 386
BseSI GKGCMC 3 cut(s) 247, 270, 381
BsgI GTGCAG 1 cut(s) 393
Bsh1285I CGRYCG 1 cut(s) 87
BshNI GGYRCC 2 cut(s) 244, 267
BsiEI CGRYCG 1 cut(s) 87
BslI CCNNNNNNNGG 1 cut(s) 342
Bsp1286I GDGCHC 3 cut(s) 247, 270, 381
Bsp143I GATC 3 cut(s) 13, 84, 300
BspLI GGNNCC 4 cut(s) 95, 170, 246, 269
BspPI GGATC 1 cut(s) 308
BspT107I GGYRCC 2 cut(s) 244, 267
BsrI ACTGG 3 cut(s) 97, 332, 386
BssMI GATC 3 cut(s) 13, 84, 300
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 3 cut(s) 229, 307, 403
BstC8I GCNNGC 4 cut(s) 31, 76, 119, 123
BstF5I GGATG 1 cut(s) 414
BstKTI GATC 3 cut(s) 16, 87, 303
BstMBI GATC 3 cut(s) 13, 84, 300
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 212
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 210
BstSLI GKGCMC 3 cut(s) 247, 270, 381
BsuI GTATCC 1 cut(s) 145
BtsCI GGATG 1 cut(s) 414
BtsIMutI CAGTG 4 cut(s) 104, 325, 376, 379
Cac8I GCNNGC 4 cut(s) 31, 76, 119, 123
Csp6I GTAC 1 cut(s) 434
CviAII CATG 1 cut(s) 118
CviJI RGCY 1 cut(s) 291
CviKI_1 RGCY 1 cut(s) 291
CviQI GTAC 1 cut(s) 434
DpnI GATC 3 cut(s) 15, 86, 302
DpnII GATC 3 cut(s) 13, 84, 300
EcoRII CCWGG 1 cut(s) 210
EcoT22I ATGCAT 1 cut(s) 286
FaeI CATG 1 cut(s) 121
FaiI YATR 3 cut(s) 119, 156, 414
FatI CATG 1 cut(s) 117
FbaI TGATCA 1 cut(s) 13
FblI GTMKAC 1 cut(s) 315
FokI GGATG 1 cut(s) 421
FspBI CTAG 1 cut(s) 147
Hin1II CATG 1 cut(s) 121
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 4 cut(s) 80, 104, 150, 207
Hpy166II GTNNAC 1 cut(s) 316
Hpy188I TCNGA 2 cut(s) 89, 363
Hpy188III TCNNGA 2 cut(s) 147, 298
Hpy8I GTNNAC 1 cut(s) 316
HpyAV CCTTC 3 cut(s) 84, 259, 433
HpyCH4III ACNGT 3 cut(s) 229, 307, 403
HpyCH4V TGCA 4 cut(s) 117, 125, 284, 374
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
Hsp92II CATG 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 13
Kzo9I GATC 3 cut(s) 13, 84, 300
LmnI GCTCC 1 cut(s) 350
LweI GCATC 1 cut(s) 293
MaeI CTAG 1 cut(s) 147
MalI GATC 3 cut(s) 15, 86, 302
MboI GATC 3 cut(s) 13, 84, 300
MhlI GDGCHC 3 cut(s) 247, 270, 381
MluCI AATT 1 cut(s) 320
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 2 cut(s) 162, 328
MnlI CCTC 2 cut(s) 233, 385
Mph1103I ATGCAT 1 cut(s) 286
MseI TTAA 2 cut(s) 24, 309
MspR9I CCNGG 1 cut(s) 212
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 118
NdeII GATC 3 cut(s) 13, 84, 300
NlaIII CATG 1 cut(s) 121
NlaIV GGNNCC 4 cut(s) 95, 170, 246, 269
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 121
PaeI GCATGC 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 58
PfeI GAWTC 3 cut(s) 104, 150, 207
PflMI CCANNNNNTGG 1 cut(s) 342
PfoI TCCNGGA 1 cut(s) 210
Ple19I CGATCG 1 cut(s) 87
PleI GAGTC 1 cut(s) 74
PpsI GAGTC 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 210
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 4 cut(s) 95, 170, 246, 269
PvuI CGATCG 1 cut(s) 87
RsaI GTAC 1 cut(s) 435
RsaNI GTAC 1 cut(s) 434
SalI GTCGAC 1 cut(s) 314
SaqAI TTAA 2 cut(s) 24, 309
Sau3AI GATC 3 cut(s) 13, 84, 300
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 1 cut(s) 212
SduI GDGCHC 3 cut(s) 247, 270, 381
SetI ASST 4 cut(s) 23, 193, 225, 315
SfaNI GCATC 1 cut(s) 293
SphI GCATGC 1 cut(s) 121
Sse9I AATT 1 cut(s) 320
SspI AATATT 1 cut(s) 236
SspMI CTAG 1 cut(s) 147
StyD4I CCNGG 1 cut(s) 210
TaaI ACNGT 3 cut(s) 229, 307, 403
TaqI TCGA 3 cut(s) 52, 83, 315
TasI AATT 1 cut(s) 320
TfiI GAWTC 3 cut(s) 104, 150, 207
Tru1I TTAA 2 cut(s) 24, 309
Tru9I TTAA 2 cut(s) 24, 309
TscAI CASTG 4 cut(s) 104, 332, 376, 386
TspDTI ATGAA 1 cut(s) 171
TspRI CASTG 4 cut(s) 104, 332, 376, 386
Van91I CCANNNNNTGG 1 cut(s) 342
XbaI TCTAGA 1 cut(s) 146
XceI RCATGY 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 315
XspI CTAG 1 cut(s) 147
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.