AT5G06410

Iron-sulfur cluster co-chaperone protein HscB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004741
Physical Location & Seq
Reverse (-)
1959367 .. 1961158
1792 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G06410.1

Sequence Viewer

Length: 759 bp
ATGAAGAAAACGAAGACGATGGTGGCTTCCATCTCCACCCTTATTAGACGGACCTATCCTTCAACTAACCAGTGTAATTCACTCGCGACGATTCAATCACAAACCCAATTGCCTCGCGAATCTCTGCAACATCATTCTTCAGCTGAGGGACGACTCCGATTCTCCGGGAGAGTTTTCTGCTCGGAATCTGGCGCCGGATGCTGGAACTGTGGCGAGAAAGCTGCCTTTTTGTTCTGTAACTCATGCCGTAGCATTCAACCTGTTGATGACTCCGTCGACTATTTTCAGATTTTTGGCCTGGAGAAGAAGTATGAGATAGATCCTGGAAGCCTTGAAGGCAAGTACAAAGATTGGCAAAAGAAGCTACATCCTGATTTAGTTCATAACAAATCTAAGAAAGAACGAGACTACGCAGCCGAGCAGTCTGCAAAGGTGACTGAAGCGTGCCGGACATTAACAAAGCGGCTGTCGAGAGCAATGTATATCATGAAGCTGAATGGTGTAAATGTCAATGAAGAAGAAACAATAACAGACCCAACATTGTTAATGGAGATTATGGAACTCAGAGAGGCCATATCAGAAGCAGATGATTCAACATCGTTAAACCAGATCCGATCCCAGGTACAAGAGAAACTAAAGCAATGGTCTGACTCTTTCGTCGAAGCGTTTGAAAGCCAGAAGTTTGATGATGCTGTAAAATGTATACAGAGAATGACTTATTACGAGCGAGCCTGCGAAGAAATTTTGAAGAAGCTATGA

Protein Analysis

252

Amino Acids

28.93

Weight (kDa)

7.51

Isoelectric Point (pI)

48.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSCB_C PF07743 178 - 248 2.7e-18 HSCB C-terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 656
AccB1I GGYRCC 1 cut(s) 191
AccI GTMKAC 2 cut(s) 276, 703
AccII CGCG 2 cut(s) 86, 117
AciI CCGC 1 cut(s) 463
AclWI GGATC 3 cut(s) 314, 604, 609
AcsI RAATTY 1 cut(s) 741
AcuI CTGAAG 2 cut(s) 123, 459
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 2 cut(s) 344, 624
AfiI CCNNNNNNNGG 2 cut(s) 201, 619
AgsI TTSAA 7 cut(s) 63, 95, 257, 335, 594, 671, 748
AjnI CCWGG 3 cut(s) 297, 322, 618
AluBI AGCT 5 cut(s) 143, 221, 364, 493, 754
AluI AGCT 5 cut(s) 143, 221, 364, 493, 754
Alw26I GTCTC 1 cut(s) 399
AlwI GGATC 3 cut(s) 314, 604, 609
AoxI GGCC 2 cut(s) 295, 570
ApeKI GCWGC 2 cut(s) 221, 413
ApoI RAATTY 1 cut(s) 741
ArsI GACNNNNNNTTYG 2 cut(s) 452, 484
AspLEI GCGC 1 cut(s) 194
AspS9I GGNCC 1 cut(s) 51
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 445
AvaII GGWCC 1 cut(s) 51
BanI GGYRCC 1 cut(s) 191
BbsI GAAGAC 1 cut(s) 20
BbvCI CCTCAGC 1 cut(s) 144
BbvI GCAGC 2 cut(s) 208, 425
BccI CCATC 2 cut(s) 13, 38
BceAI ACGGC 1 cut(s) 231
BcgI CGANNNNNNTGC 4 cut(s) 203, 237, 407, 441
BciT130I CCWGG 3 cut(s) 299, 324, 620
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 399
BfoI RGCGCY 1 cut(s) 195
BglI GCCNNNNNGGC 1 cut(s) 336
BisI GCNGC 3 cut(s) 222, 414, 464
BlsI GCNGC 3 cut(s) 223, 415, 465
Bme1390I CCNGG 4 cut(s) 166, 299, 324, 620
Bme18I GGWCC 1 cut(s) 51
BmgT120I GGNCC 1 cut(s) 51
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 4 cut(s) 166, 299, 324, 620
BmsI GCATC 2 cut(s) 188, 679
BpiI GAAGAC 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 144
BpuMI CCSGG 1 cut(s) 166
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 618
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 2 cut(s) 201, 619
Bse1I ACTGG 1 cut(s) 70
Bse3DI GCAATG 2 cut(s) 483, 647
BseBI CCWGG 3 cut(s) 299, 324, 620
BseDI CCNNGG 1 cut(s) 618
BseGI GGATG 2 cut(s) 203, 367
BseLI CCNNNNNNNGG 2 cut(s) 201, 619
BseMI GCAATG 2 cut(s) 483, 647
BseMII CTCAG 2 cut(s) 135, 577
BseNI ACTGG 1 cut(s) 70
BseXI GCAGC 2 cut(s) 208, 425
Bsh1236I CGCG 2 cut(s) 86, 117
BshFI GGCC 2 cut(s) 297, 572
BshNI GGYRCC 1 cut(s) 191
BsiSI CCGG 3 cut(s) 165, 195, 448
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 2 cut(s) 201, 619
BsmAI GTCTC 1 cut(s) 399
BsmFI GGGAC 1 cut(s) 162
BsmI GAATGC 1 cut(s) 252
BsnI GGCC 2 cut(s) 297, 572
Bsp143I GATC 3 cut(s) 319, 609, 614
Bsp68I TCGCGA 2 cut(s) 86, 117
BspACI CCGC 1 cut(s) 463
BspANI GGCC 2 cut(s) 297, 572
BspCNI CTCAG 2 cut(s) 136, 576
BspFNI CGCG 2 cut(s) 86, 117
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 1 cut(s) 193
BspPI GGATC 3 cut(s) 314, 604, 609
BspT107I GGYRCC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 483, 647
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 618
BssMI GATC 3 cut(s) 319, 609, 614
BssNAI GTATAC 1 cut(s) 704
BssNI GRCGYC 1 cut(s) 192
Bst1107I GTATAC 1 cut(s) 704
Bst2UI CCWGG 3 cut(s) 299, 324, 620
Bst4CI ACNGT 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 3 cut(s) 445, 729, 733
BstDEI CTNAG 3 cut(s) 144, 393, 563
BstF5I GGATG 2 cut(s) 203, 367
BstFNI CGCG 2 cut(s) 86, 117
BstH2I RGCGCY 1 cut(s) 195
BstHHI GCGC 1 cut(s) 194
BstKTI GATC 3 cut(s) 322, 612, 617
BstMAI GTCTC 1 cut(s) 399
BstMBI GATC 3 cut(s) 319, 609, 614
BstMWI GCNNNNNNNGC 3 cut(s) 198, 336, 361
BstNI CCWGG 3 cut(s) 299, 324, 620
BstSCI CCNGG 4 cut(s) 164, 297, 322, 618
BstUI CGCG 2 cut(s) 86, 117
BstV1I GCAGC 2 cut(s) 208, 425
BstV2I GAAGAC 1 cut(s) 20
BstX2I RGATCY 2 cut(s) 319, 609
BstYI RGATCY 2 cut(s) 319, 609
BstZ17I GTATAC 1 cut(s) 704
BsuRI GGCC 2 cut(s) 297, 572
BtsCI GGATG 2 cut(s) 203, 367
BtsIMutI CAGTG 1 cut(s) 77
BtuMI TCGCGA 2 cut(s) 86, 117
Cac8I GCNNGC 3 cut(s) 445, 729, 733
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 194
Cfr13I GGNCC 1 cut(s) 51
Csp6I GTAC 2 cut(s) 343, 623
CviAII CATG 2 cut(s) 243, 487
CviQI GTAC 2 cut(s) 343, 623
DdeI CTNAG 3 cut(s) 144, 393, 563
DinI GGCGCC 1 cut(s) 193
DpnI GATC 3 cut(s) 321, 611, 616
DpnII GATC 3 cut(s) 319, 609, 614
DrdI GACNNNNNNGTC 1 cut(s) 656
DseDI GACNNNNNNGTC 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 2 cut(s) 123, 459
EcoRII CCWGG 3 cut(s) 297, 322, 618
EgeI GGCGCC 1 cut(s) 193
EheI GGCGCC 1 cut(s) 193
FaeI CATG 2 cut(s) 246, 490
FaiI YATR 9 cut(s) 244, 312, 384, 483, 488, 557, 575, 704, 757
FaqI GGGAC 1 cut(s) 162
FatI CATG 2 cut(s) 242, 486
FblI GTMKAC 2 cut(s) 276, 703
Fnu4HI GCNGC 3 cut(s) 222, 414, 464
FokI GGATG 2 cut(s) 210, 354
Fsp4HI GCNGC 3 cut(s) 222, 414, 464
GlaI GCGC 1 cut(s) 193
GluI GCNGC 3 cut(s) 222, 414, 464
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 195
HaeIII GGCC 2 cut(s) 297, 572
HapII CCGG 3 cut(s) 165, 195, 448
HhaI GCGC 1 cut(s) 194
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 2 cut(s) 246, 490
Hin6I GCGC 1 cut(s) 192
HinP1I GCGC 1 cut(s) 192
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 8 cut(s) 91, 119, 153, 159, 185, 269, 590, 650
HpaII CCGG 3 cut(s) 165, 195, 448
HphI GGTGA 1 cut(s) 445
Hpy166II GTNNAC 2 cut(s) 277, 704
Hpy188I TCNGA 7 cut(s) 158, 184, 288, 566, 580, 614, 649
Hpy188III TCNNGA 5 cut(s) 85, 116, 371, 471, 487
Hpy8I GTNNAC 2 cut(s) 277, 704
Hpy99I CGWCG 3 cut(s) 91, 278, 662
HpyAV CCTTC 2 cut(s) 69, 329
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4V TGCA 2 cut(s) 127, 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 336, 361
HpyF3I CTNAG 3 cut(s) 144, 393, 563
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 2 cut(s) 246, 490
HspAI GCGC 1 cut(s) 192
KasI GGCGCC 1 cut(s) 191
Kzo9I GATC 3 cut(s) 319, 609, 614
Lsp1109I GCAGC 2 cut(s) 208, 425
LweI GCATC 2 cut(s) 188, 679
MaeIII GTNAC 2 cut(s) 236, 433
MalI GATC 3 cut(s) 321, 611, 616
MboI GATC 3 cut(s) 319, 609, 614
MboII GAAGA 7 cut(s) 16, 25, 129, 316, 527, 530, 749
MfeI CAATTG 1 cut(s) 107
MflI RGATCY 2 cut(s) 319, 609
MluCI AATT 3 cut(s) 76, 107, 741
Mly113I GGCGCC 1 cut(s) 192
MlyI GAGTC 3 cut(s) 147, 263, 644
MnlI CCTC 3 cut(s) 123, 139, 562
MseI TTAA 3 cut(s) 455, 545, 602
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 3 cut(s) 165, 195, 448
MspR9I CCNGG 4 cut(s) 166, 299, 324, 620
MunI CAATTG 1 cut(s) 107
Mva1269I GAATGC 1 cut(s) 252
MvaI CCWGG 3 cut(s) 299, 324, 620
MvnI CGCG 2 cut(s) 86, 117
MwoI GCNNNNNNNGC 3 cut(s) 198, 336, 361
NarI GGCGCC 1 cut(s) 192
NciI CCSGG 1 cut(s) 166
NdeII GATC 3 cut(s) 319, 609, 614
NlaIII CATG 2 cut(s) 246, 490
NlaIV GGNNCC 1 cut(s) 193
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 1 cut(s) 433
NruI TCGCGA 2 cut(s) 86, 117
PagI TCATGA 1 cut(s) 486
PctI GAATGC 1 cut(s) 252
PfeI GAWTC 5 cut(s) 91, 119, 159, 185, 590
PflFI GACNNNGTC 1 cut(s) 272
PfoI TCCNGGA 2 cut(s) 164, 322
PkrI GCNGC 3 cut(s) 223, 415, 465
PleI GAGTC 3 cut(s) 147, 263, 644
PluTI GGCGCC 1 cut(s) 195
PpsI GAGTC 3 cut(s) 147, 263, 644
Psp6I CCWGG 3 cut(s) 297, 322, 618
PspGI CCWGG 3 cut(s) 297, 322, 618
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 1 cut(s) 51
PsuI RGATCY 2 cut(s) 319, 609
PsyI GACNNNGTC 1 cut(s) 272
PvuII CAGCTG 1 cut(s) 143
RruI TCGCGA 2 cut(s) 86, 117
RsaI GTAC 2 cut(s) 344, 624
RsaNI GTAC 2 cut(s) 343, 623
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 3 cut(s) 455, 545, 602
SatI GCNGC 3 cut(s) 222, 414, 464
Sau3AI GATC 3 cut(s) 319, 609, 614
Sau96I GGNCC 1 cut(s) 51
SchI GAGTC 3 cut(s) 147, 263, 644
ScrFI CCNGG 4 cut(s) 166, 299, 324, 620
SetI ASST 9 cut(s) 56, 145, 223, 262, 366, 435, 495, 624, 756
SfaNI GCATC 2 cut(s) 188, 679
SfoI GGCGCC 1 cut(s) 193
SinI GGWCC 1 cut(s) 51
Sse9I AATT 3 cut(s) 76, 107, 741
SsiI CCGC 1 cut(s) 463
SspDI GGCGCC 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 164, 297, 322, 618
TaaI ACNGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 276, 470, 660
TasI AATT 3 cut(s) 76, 107, 741
TatI WGTACW 1 cut(s) 342
TauI GCSGC 1 cut(s) 466
TfiI GAWTC 5 cut(s) 91, 119, 159, 185, 590
Tru1I TTAA 3 cut(s) 455, 545, 602
Tru9I TTAA 3 cut(s) 455, 545, 602
TscAI CASTG 1 cut(s) 77
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 221, 413
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 4 cut(s) 17, 371, 503, 528
TspGWI ACGGA 2 cut(s) 64, 262
TspRI CASTG 1 cut(s) 77
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 51
XapI RAATTY 1 cut(s) 741
XmiI GTMKAC 2 cut(s) 276, 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.