RchiOBHm_Chr3g0476751

Iron-sulfur cluster co-chaperone protein HscB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0004741
Physical Location & Seq
Forward (+)
22730916 .. 22735177
4262 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44214

Sequence Viewer

Length: 792 bp
ATGTCGAAGAAGAAGCTACTGACTCCTCTCTCCTCTCTTCTCCTCCTCCGACGAACCCTAACTTCCACCCCTCATTTCTCAATCCCACACTTCCAATCCCGCACCAATTTCTCCCCCAATATCGAAAAGCTCCGCCCCCTTTTCCCGCTCCCCGGATTTGATTTTCCCGGCAAATCTTTCATCTCCTCCCAATTTGGGAACAAACCCAATTCCCCCACTGCTGGAACTGCAGCGCTGCCGCTCAAACGACGCCTTTTCTGCTCTGCCCCTCCTGTCGGTGCATCCAACCCGTCGATCACTCCGTCGATTACTTTCAGATATTTGGACTGTAAGGAGAGGAAGTATGATAGTGTGGAGGAACATCTGGAGGGAAAATATAAGGATTGGCAGAAAAAGTTGCACCCTGATCTTGTTCATTCAAAATCAGAGAAGGAGAGAGAGTACGCTGCTGAACAGTCTGCTCGGGTGATTGATGCATACCGCACGCTTAGCAAGCCCTTGGCGAGGGCAATCTATATTCTGAAGCTTGAAGGTGTAGACATCGATGAAGAAGAGACACTTTCAGACTTAGATTTGTTAAGTGAGATTTTGGAAGTAAGGGAAGCTGTTGAAGAGGCTCCCAACTCTCAGGCTTTAAATCAGATTCAGTCTCTGATGCAAGAGAAACTGAAGCAGTGGTCTAATTCTTTTGCCGAGGCATTTGAAAGCCGGAACTTTGAAGAAGCCGTAAAAGCTATCCGGCGAATGACTTACTATGAGCGTGTAAATGAAGAAATTGTAAAGAGGCAATGA

Protein Analysis

263

Amino Acids

30.18

Weight (kDa)

8.69

Isoelectric Point (pI)

55.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSCB_C PF07743 189 - 259 7.8e-16 HSCB C-terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 148, 241
AccI GTMKAC 1 cut(s) 537
AciI CCGC 5 cut(s) 100, 133, 146, 239, 481
AcuI CTGAAG 2 cut(s) 542, 689
AcyI GRCGYC 1 cut(s) 250
AfaI GTAC 1 cut(s) 443
AfeI AGCGCT 1 cut(s) 234
AfiI CCNNNNNNNGG 5 cut(s) 152, 195, 221, 275, 504
AgsI TTSAA 5 cut(s) 420, 530, 611, 704, 719
AluBI AGCT 5 cut(s) 16, 130, 526, 605, 734
AluI AGCT 5 cut(s) 16, 130, 526, 605, 734
Alw26I GTCTC 2 cut(s) 548, 654
AlwNI CAGNNNCTG 1 cut(s) 652
Ama87I CYCGRG 1 cut(s) 462
Aor51HI AGCGCT 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 230, 235, 446
AspLEI GCGC 1 cut(s) 235
AsuC2I CCSGG 2 cut(s) 153, 168
AsuHPI GGTGA 1 cut(s) 478
AvaI CYCGRG 1 cut(s) 462
BbvI GCAGC 3 cut(s) 222, 242, 433
BceAI ACGGC 1 cut(s) 710
BcnI CCSGG 2 cut(s) 153, 168
BcoDI GTCTC 2 cut(s) 548, 654
BfmI CTRYAG 1 cut(s) 228
BfoI RGCGCY 1 cut(s) 236
BisI GCNGC 4 cut(s) 231, 236, 239, 447
BlpI GCTNAGC 1 cut(s) 488
BlsI GCNGC 4 cut(s) 232, 237, 240, 448
Bme1390I CCNGG 2 cut(s) 153, 168
BmeT110I CYCGRG 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 618
BmrFI CCNGG 2 cut(s) 153, 168
BmsI GCATC 3 cut(s) 290, 463, 645
BpmI CTGGAG 1 cut(s) 386
Bpu1102I GCTNAGC 1 cut(s) 488
BpuMI CCSGG 2 cut(s) 153, 168
Bsa29I ATCGAT 1 cut(s) 543
BsaHI GRCGYC 1 cut(s) 250
BsaJI CCNNGG 3 cut(s) 151, 498, 693
BsaXI ACNNNNNCTCC 4 cut(s) 326, 356, 425, 455
Bsc4I CCNNNNNNNGG 5 cut(s) 152, 195, 221, 275, 504
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 3 cut(s) 151, 498, 693
BseGI GGATG 1 cut(s) 281
BseLI CCNNNNNNNGG 5 cut(s) 152, 195, 221, 275, 504
BseMII CTCAG 1 cut(s) 641
BseRI GAGGAG 5 cut(s) 15, 22, 32, 35, 175
BseXI GCAGC 3 cut(s) 222, 242, 433
BshVI ATCGAT 1 cut(s) 543
BsiHKCI CYCGRG 1 cut(s) 462
BsiSI CCGG 4 cut(s) 153, 168, 709, 739
BslI CCNNNNNNNGG 5 cut(s) 152, 195, 221, 275, 504
BsmAI GTCTC 2 cut(s) 548, 654
BsoBI CYCGRG 1 cut(s) 462
Bsp143I GATC 2 cut(s) 294, 406
Bsp1720I GCTNAGC 1 cut(s) 488
BspACI CCGC 5 cut(s) 100, 133, 146, 239, 481
BspCNI CTCAG 1 cut(s) 640
BspDI ATCGAT 1 cut(s) 543
BspLI GGNNCC 1 cut(s) 618
BspMAI CTGCAG 1 cut(s) 232
BsrBI CCGCTC 2 cut(s) 148, 241
BssECI CCNNGG 3 cut(s) 151, 498, 693
BssMI GATC 2 cut(s) 294, 406
BssNI GRCGYC 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 498
Bst4CI ACNGT 2 cut(s) 329, 456
Bst6I CTCTTC 3 cut(s) 42, 546, 606
BstACI GRCGYC 1 cut(s) 250
BstC8I GCNNGC 2 cut(s) 485, 494
BstDEI CTNAG 3 cut(s) 488, 568, 627
BstENI CCTNNNNNAGG 1 cut(s) 502
BstF5I GGATG 1 cut(s) 281
BstH2I RGCGCY 1 cut(s) 236
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 2 cut(s) 297, 409
BstMAI GTCTC 2 cut(s) 548, 654
BstMBI GATC 2 cut(s) 294, 406
BstMWI GCNNNNNNNGC 5 cut(s) 227, 258, 489, 493, 731
BstSCI CCNGG 2 cut(s) 151, 166
BstSFI CTRYAG 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 222, 242, 433
Bsu15I ATCGAT 1 cut(s) 543
BsuTUI ATCGAT 1 cut(s) 543
BtsCI GGATG 1 cut(s) 281
BtsI GCAGTG 2 cut(s) 216, 680
BtsIMutI CAGTG 2 cut(s) 216, 680
Cac8I GCNNGC 2 cut(s) 485, 494
CaiI CAGNNNCTG 1 cut(s) 652
CfoI GCGC 1 cut(s) 235
ClaI ATCGAT 1 cut(s) 543
CseI GACGC 1 cut(s) 258
Csp6I GTAC 1 cut(s) 442
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 3 cut(s) 488, 568, 627
DpnI GATC 2 cut(s) 296, 408
DpnII GATC 2 cut(s) 294, 406
DraI TTTAAA 1 cut(s) 636
Eam1104I CTCTTC 3 cut(s) 42, 546, 606
EarI CTCTTC 3 cut(s) 42, 546, 606
EciI GGCGGA 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 498
Eco47III AGCGCT 1 cut(s) 234
Eco57I CTGAAG 2 cut(s) 542, 689
Eco88I CYCGRG 1 cut(s) 462
EcoNI CCTNNNNNAGG 1 cut(s) 502
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 478
ErhI CCWWGG 1 cut(s) 498
FaiI YATR 5 cut(s) 345, 378, 478, 516, 756
FalI AAGNNNNNCTT 2 cut(s) 543, 575
FauI CCCGC 2 cut(s) 107, 153
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 4 cut(s) 231, 236, 239, 447
FokI GGATG 1 cut(s) 268
Fsp4HI GCNGC 4 cut(s) 231, 236, 239, 447
GlaI GCGC 1 cut(s) 234
GluI GCNGC 4 cut(s) 231, 236, 239, 447
GsuI CTGGAG 1 cut(s) 386
HaeII RGCGCY 1 cut(s) 236
HapII CCGG 4 cut(s) 153, 168, 709, 739
HgaI GACGC 1 cut(s) 258
HhaI GCGC 1 cut(s) 235
Hin1I GRCGYC 1 cut(s) 250
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 2 cut(s) 22, 643
HpaII CCGG 4 cut(s) 153, 168, 709, 739
HphI GGTGA 1 cut(s) 478
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 7 cut(s) 50, 317, 427, 522, 565, 642, 654
Hpy188III TCNNGA 1 cut(s) 365
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 4 cut(s) 54, 252, 295, 307
HpyAV CCTTC 2 cut(s) 424, 524
HpyCH4III ACNGT 2 cut(s) 329, 456
HpyCH4V TGCA 5 cut(s) 230, 281, 400, 476, 658
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 258, 489, 493, 731
HpyF3I CTNAG 3 cut(s) 488, 568, 627
Hsp92I GRCGYC 1 cut(s) 250
HspAI GCGC 1 cut(s) 233
Kzo9I GATC 2 cut(s) 294, 406
LmnI GCTCC 3 cut(s) 135, 153, 622
LpnPI CCDG 9 cut(s) 166, 181, 207, 285, 350, 417, 614, 722, 752
Lsp1109I GCAGC 3 cut(s) 222, 242, 433
LweI GCATC 3 cut(s) 290, 463, 645
MalI GATC 2 cut(s) 296, 408
MbiI CCGCTC 2 cut(s) 148, 241
MboI GATC 2 cut(s) 294, 406
MboII GAAGA 8 cut(s) 19, 22, 29, 560, 563, 623, 731, 782
MluCI AATT 5 cut(s) 106, 191, 208, 682, 774
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 73, 309
Mph1103I ATGCAT 1 cut(s) 478
MseI TTAA 2 cut(s) 578, 635
MspI CCGG 4 cut(s) 153, 168, 709, 739
MspR9I CCNGG 2 cut(s) 153, 168
MwoI GCNNNNNNNGC 5 cut(s) 227, 258, 489, 493, 731
NciI CCSGG 2 cut(s) 153, 168
NdeII GATC 2 cut(s) 294, 406
NlaIV GGNNCC 1 cut(s) 618
NmeAIII GCCGAG 1 cut(s) 718
NsiI ATGCAT 1 cut(s) 478
PcsI WCGNNNNNNNCGW 1 cut(s) 299
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 4 cut(s) 232, 237, 240, 448
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 618
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 652
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SaqAI TTAA 2 cut(s) 578, 635
SatI GCNGC 4 cut(s) 231, 236, 239, 447
Sau3AI GATC 2 cut(s) 294, 406
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 153, 168
SetI ASST 6 cut(s) 18, 132, 528, 535, 607, 736
SfaNI GCATC 3 cut(s) 290, 463, 645
SfcI CTRYAG 1 cut(s) 228
Sse9I AATT 5 cut(s) 106, 191, 208, 682, 774
SsiI CCGC 5 cut(s) 100, 133, 146, 239, 481
StyD4I CCNGG 2 cut(s) 151, 166
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 329, 456
TaqI TCGA 5 cut(s) 5, 123, 293, 305, 543
TasI AATT 5 cut(s) 106, 191, 208, 682, 774
TauI GCSGC 1 cut(s) 241
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 2 cut(s) 578, 635
Tru9I TTAA 2 cut(s) 578, 635
TscAI CASTG 2 cut(s) 223, 680
TseI GCWGC 3 cut(s) 230, 235, 446
TspDTI ATGAA 4 cut(s) 169, 404, 561, 783
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 223, 680
XagI CCTNNNNNAGG 1 cut(s) 502
XmiI GTMKAC 1 cut(s) 537
Zsp2I ATGCAT 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.