Rh3CG233400

Iron-sulfur cluster co-chaperone protein HscB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0004741
Physical Location & Seq
Forward (+)
22040558 .. 22044550
3993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG233400.1

Sequence Viewer

Length: 657 bp
ATGTCGAAGAAGAAGCTACTGACTCCTCTCTCCTCTCTTCTCCTCCTCCGACGAACCCTAACTTCCACCCCTCATTTCTCAATCCCATACTTGCAATCCCGCACCAATTTCTCCCCCAATATCGAAAAGCCCCGCCCCCTTTTCCCCCTCCCCGGATTTGATTTTCCCGGCAAATCTTTCATCTCCTCCCAATTTGGGGACAAACCCAATTCCGCCCACTGCTGGAACTGCAGCGCTGCCGCTCGAACGACGCCGTTTCTGCTCTGCTTCTCCTGTCGGTGCATCCAACCCGTCGATCACTCCGTCGATTACTTTCAGATATTTGGACTGGAGAGGAAGTATGATAGTGTGGAGGAACATCTGGAGGGAAAATATAAGGATTGGCAGAAAAAGTTGCACCCTGATCTTGTTCATTCAAAATCAGAGAAGGAGAGAGAGTACGCTGCTGAACAGTCTGCTCGCGTGATTGATGCATACCGCACGCTTAGCAAGCCCTTGGCGAGGGCAATATATATTCTGAAGCTTGAAGGTGTAGACATCGATGAAGAAGAGACACTTTCAGACTTAGATTTGTTAAGTGAGATTATGGAGGTAAGGGAAGCTGTTGAAGAGGCTCCCAACTCTCAGGCTTTAAATCAGATTCAGTCTCTGGTATGA

Protein Analysis

218

Amino Acids

24.92

Weight (kDa)

6.09

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 103 - 171 5.5e-06 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 534
AccII CGCG 1 cut(s) 462
AciI CCGC 5 cut(s) 100, 133, 213, 240, 478
AcuI CTGAAG 1 cut(s) 539
AcyI GRCGYC 1 cut(s) 251
AfaI GTAC 1 cut(s) 440
AfeI AGCGCT 1 cut(s) 235
AfiI CCNNNNNNNGG 5 cut(s) 152, 195, 196, 222, 501
AgsI TTSAA 3 cut(s) 417, 527, 608
AluBI AGCT 3 cut(s) 16, 523, 602
AluI AGCT 3 cut(s) 16, 523, 602
Alw26I GTCTC 2 cut(s) 545, 651
AlwNI CAGNNNCTG 1 cut(s) 649
Aor51HI AGCGCT 1 cut(s) 235
ApeKI GCWGC 3 cut(s) 231, 236, 443
AspLEI GCGC 1 cut(s) 236
AsuC2I CCSGG 2 cut(s) 153, 168
BbvI GCAGC 3 cut(s) 223, 243, 430
BceAI ACGGC 1 cut(s) 238
BcnI CCSGG 2 cut(s) 153, 168
BcoDI GTCTC 2 cut(s) 545, 651
BfmI CTRYAG 1 cut(s) 229
BfoI RGCGCY 1 cut(s) 237
BisI GCNGC 4 cut(s) 232, 237, 240, 444
BlpI GCTNAGC 1 cut(s) 485
BlsI GCNGC 4 cut(s) 233, 238, 241, 445
Bme1390I CCNGG 2 cut(s) 153, 168
BmiI GGNNCC 1 cut(s) 615
BmrFI CCNGG 2 cut(s) 153, 168
BmsI GCATC 2 cut(s) 291, 460
BpmI CTGGAG 2 cut(s) 350, 383
Bpu1102I GCTNAGC 1 cut(s) 485
BpuMI CCSGG 2 cut(s) 153, 168
Bsa29I ATCGAT 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 151, 495
BsaXI ACNNNNNCTCC 4 cut(s) 323, 353, 422, 452
Bsc4I CCNNNNNNNGG 5 cut(s) 152, 195, 196, 222, 501
Bse1I ACTGG 1 cut(s) 333
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 151, 495
BseGI GGATG 1 cut(s) 282
BseLI CCNNNNNNNGG 5 cut(s) 152, 195, 196, 222, 501
BseMII CTCAG 1 cut(s) 638
BseNI ACTGG 1 cut(s) 333
BseRI GAGGAG 5 cut(s) 15, 22, 32, 35, 175
BseXI GCAGC 3 cut(s) 223, 243, 430
Bsh1236I CGCG 1 cut(s) 462
BshVI ATCGAT 1 cut(s) 540
BsiSI CCGG 2 cut(s) 153, 168
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 5 cut(s) 152, 195, 196, 222, 501
BsmAI GTCTC 2 cut(s) 545, 651
BsmFI GGGAC 1 cut(s) 212
Bsp143I GATC 2 cut(s) 295, 403
Bsp1720I GCTNAGC 1 cut(s) 485
BspACI CCGC 5 cut(s) 100, 133, 213, 240, 478
BspCNI CTCAG 1 cut(s) 637
BspDI ATCGAT 1 cut(s) 540
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 1 cut(s) 615
BspMAI CTGCAG 1 cut(s) 233
BsrBI CCGCTC 1 cut(s) 242
BsrI ACTGG 1 cut(s) 333
BssECI CCNNGG 2 cut(s) 151, 495
BssMI GATC 2 cut(s) 295, 403
BssNI GRCGYC 1 cut(s) 251
BssT1I CCWWGG 1 cut(s) 495
Bst4CI ACNGT 1 cut(s) 453
Bst6I CTCTTC 3 cut(s) 42, 543, 603
BstACI GRCGYC 1 cut(s) 251
BstC8I GCNNGC 3 cut(s) 460, 482, 491
BstDEI CTNAG 3 cut(s) 485, 565, 624
BstENI CCTNNNNNAGG 1 cut(s) 499
BstF5I GGATG 1 cut(s) 282
BstFNI CGCG 1 cut(s) 462
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 2 cut(s) 298, 406
BstMAI GTCTC 2 cut(s) 545, 651
BstMBI GATC 2 cut(s) 295, 403
BstMWI GCNNNNNNNGC 4 cut(s) 228, 259, 486, 490
BstSCI CCNGG 2 cut(s) 151, 166
BstSFI CTRYAG 1 cut(s) 229
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 3 cut(s) 223, 243, 430
Bsu15I ATCGAT 1 cut(s) 540
BsuTUI ATCGAT 1 cut(s) 540
BtsCI GGATG 1 cut(s) 282
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 3 cut(s) 460, 482, 491
CaiI CAGNNNCTG 1 cut(s) 649
CfoI GCGC 1 cut(s) 236
ClaI ATCGAT 1 cut(s) 540
CseI GACGC 1 cut(s) 259
Csp6I GTAC 1 cut(s) 439
CviJI RGCY 7 cut(s) 16, 130, 493, 523, 602, 614, 629
CviKI_1 RGCY 7 cut(s) 16, 130, 493, 523, 602, 614, 629
CviQI GTAC 1 cut(s) 439
DdeI CTNAG 3 cut(s) 485, 565, 624
DpnI GATC 2 cut(s) 297, 405
DpnII GATC 2 cut(s) 295, 403
DraI TTTAAA 1 cut(s) 633
Eam1104I CTCTTC 3 cut(s) 42, 543, 603
EarI CTCTTC 3 cut(s) 42, 543, 603
EciI GGCGGA 1 cut(s) 202
Eco130I CCWWGG 1 cut(s) 495
Eco47III AGCGCT 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 475
ErhI CCWWGG 1 cut(s) 495
FaiI YATR 8 cut(s) 88, 342, 375, 475, 511, 513, 587, 655
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 1 cut(s) 212
FauI CCCGC 2 cut(s) 107, 140
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 4 cut(s) 232, 237, 240, 444
FokI GGATG 1 cut(s) 269
Fsp4HI GCNGC 4 cut(s) 232, 237, 240, 444
GlaI GCGC 1 cut(s) 235
GluI GCNGC 4 cut(s) 232, 237, 240, 444
GsuI CTGGAG 2 cut(s) 350, 383
HaeII RGCGCY 1 cut(s) 237
HapII CCGG 2 cut(s) 153, 168
HgaI GACGC 1 cut(s) 259
HhaI GCGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 251
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 2 cut(s) 22, 640
HpaII CCGG 2 cut(s) 153, 168
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 6 cut(s) 50, 318, 424, 519, 562, 639
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 535
Hpy99I CGWCG 4 cut(s) 54, 253, 296, 308
HpyAV CCTTC 2 cut(s) 421, 521
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4V TGCA 5 cut(s) 94, 231, 282, 397, 473
HpyF10VI GCNNNNNNNGC 4 cut(s) 228, 259, 486, 490
HpyF3I CTNAG 3 cut(s) 485, 565, 624
Hsp92I GRCGYC 1 cut(s) 251
HspAI GCGC 1 cut(s) 234
Kzo9I GATC 2 cut(s) 295, 403
LmnI GCTCC 1 cut(s) 619
LpnPI CCDG 9 cut(s) 166, 181, 208, 286, 314, 347, 414, 611, 635
Lsp1109I GCAGC 3 cut(s) 223, 243, 430
LweI GCATC 2 cut(s) 291, 460
MalI GATC 2 cut(s) 297, 405
MbiI CCGCTC 1 cut(s) 242
MboI GATC 2 cut(s) 295, 403
MboII GAAGA 6 cut(s) 19, 22, 29, 557, 560, 620
MluCI AATT 3 cut(s) 106, 191, 208
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 73, 310
Mph1103I ATGCAT 1 cut(s) 475
MseI TTAA 2 cut(s) 575, 632
MspI CCGG 2 cut(s) 153, 168
MspR9I CCNGG 2 cut(s) 153, 168
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 4 cut(s) 228, 259, 486, 490
NciI CCSGG 2 cut(s) 153, 168
NdeII GATC 2 cut(s) 295, 403
NlaIV GGNNCC 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 1 cut(s) 640
PkrI GCNGC 4 cut(s) 233, 238, 241, 445
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PspN4I GGNNCC 1 cut(s) 615
PstI CTGCAG 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 649
RsaI GTAC 1 cut(s) 440
RsaNI GTAC 1 cut(s) 439
SaqAI TTAA 2 cut(s) 575, 632
SatI GCNGC 4 cut(s) 232, 237, 240, 444
Sau3AI GATC 2 cut(s) 295, 403
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 2 cut(s) 153, 168
SetI ASST 5 cut(s) 18, 525, 532, 594, 604
SfaNI GCATC 2 cut(s) 291, 460
SfcI CTRYAG 1 cut(s) 229
Sse9I AATT 3 cut(s) 106, 191, 208
SsiI CCGC 5 cut(s) 100, 133, 213, 240, 478
StyD4I CCNGG 2 cut(s) 151, 166
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 1 cut(s) 453
TaqI TCGA 6 cut(s) 5, 123, 244, 294, 306, 540
TasI AATT 3 cut(s) 106, 191, 208
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 1 cut(s) 640
Tru1I TTAA 2 cut(s) 575, 632
Tru9I TTAA 2 cut(s) 575, 632
TscAI CASTG 1 cut(s) 224
TseI GCWGC 3 cut(s) 231, 236, 443
TspDTI ATGAA 3 cut(s) 169, 401, 558
TspGWI ACGGA 1 cut(s) 292
TspRI CASTG 1 cut(s) 224
XagI CCTNNNNNAGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 534
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.