MD12G1044000.v1.1

Iron-sulfur cluster co-chaperone protein HscB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004741
Physical Location & Seq
Forward (+)
4946898 .. 4951233
4336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1044000.v1.1.491

Sequence Viewer

Length: 891 bp
ATGTCGACGAAGAAGCTGCTCACTCCTCTCTCCGCCATTCTCCGGCGAACCCTAACTCCGACTACCGCTCTTTTCACCTTCAACTCCTCAAGAAATTTCCAATCTCCCGCTTTTTTTTCGTCCAGTGTAGAAGCGGACCGCCGCCTTTTCCCGTTCGGCGGATTTGGTTTTCCCGGGAATCACAGAATTCCCGGGAAATGCTTCGTCAGCTCCCAATCGGCGGAGAAAGCCGACGCTAAGTGCTGGAACTGTAGTGCCTCGCCCGAAGCGGTGCCGTTTCTGTTGTGTTCGTCTTGTCGGTGCATTCAGCCGGTGGATCAGTCAGTGGATTACTTTCAGATATTTGGACTGGAGAGAAAGTACGATACCAAAGTGGACGATTTGGAGGGGAAGTATAAAGCCTGGCAGAAAAAACTGCACCCTGATCTTGTTCATACTAAATCAGAGAAAGAAAGAGAGTATGCAGCTGAACAGTCTGCTCGGGTGATTGATGCATACCGCACACTAAGCCAGCCCTTGGCAAGGGCAATTTACATTCTGAAGCTTGAAGGTGTGGACGTTGATGAAGAAGAGACGCTTTCAGAATCAGAGTTGCTAACTGAGATTTTGGAAATCAGGGAAGCTGTTGAAGAGGCTGCTGACTCTCAGGCTTTGAATGAGATTCAATCTCAGATGAAAGAGAAACTAAAGCATTGGTCCGACATTTTTACAAACGCATTTCGTAGCCGCAACTTTGGAGAGGCTCTAAAAGCTATCCGGAGAATGACTTACTATGAGCGTGTAAATGAAAGTGTTGCTCTTAGGATCTCAAACAGTATGGTTCTTGAGCAAACAAAGTTTAACTGCACCATTGACAAGAAGATTTCGCAACAAAGTCCAGGTTTTAATTGA

Protein Analysis

297

Amino Acids

33.7

Weight (kDa)

6.91

Isoelectric Point (pI)

56.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSCB_C PF07743 195 - 265 2.2e-15 HSCB C-terminal oligomerisation domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 517
AccBSI CCGCTC 1 cut(s) 68
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 756
AclWI GGATC 2 cut(s) 324, 812
AcsI RAATTY 2 cut(s) 94, 186
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 1 cut(s) 362
AfiI CCNNNNNNNGG 5 cut(s) 42, 158, 220, 517, 522
AgsI TTSAA 5 cut(s) 82, 548, 629, 655, 665
AjnI CCWGG 2 cut(s) 401, 877
AloI GAACNNNNNNTCC 2 cut(s) 40, 72
AluBI AGCT 6 cut(s) 16, 210, 467, 544, 623, 752
AluI AGCT 6 cut(s) 16, 210, 467, 544, 623, 752
Alw26I GTCTC 1 cut(s) 566
AlwI GGATC 2 cut(s) 324, 812
Ama87I CYCGRG 3 cut(s) 173, 191, 480
Aor13HI TCCGGA 1 cut(s) 756
ApeKI GCWGC 3 cut(s) 16, 464, 635
ApoI RAATTY 2 cut(s) 94, 186
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 2 cut(s) 136, 696
AsuC2I CCSGG 4 cut(s) 174, 175, 192, 193
AsuHPI GGTGA 2 cut(s) 67, 496
AvaI CYCGRG 3 cut(s) 173, 191, 480
AvaII GGWCC 2 cut(s) 136, 696
BaeI ACNNNNGTAYC 2 cut(s) 357, 390
BanI GGYRCC 1 cut(s) 271
BbvI GCAGC 3 cut(s) 3, 476, 622
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 2 cut(s) 403, 879
BcnI CCSGG 4 cut(s) 174, 175, 192, 193
BcoDI GTCTC 1 cut(s) 566
BfmI CTRYAG 1 cut(s) 250
BisI GCNGC 5 cut(s) 17, 142, 465, 636, 727
BlsI GCNGC 5 cut(s) 18, 143, 466, 637, 728
Bme1390I CCNGG 6 cut(s) 174, 175, 192, 193, 403, 879
Bme18I GGWCC 2 cut(s) 136, 696
BmeT110I CYCGRG 3 cut(s) 173, 191, 480
BmgT120I GGNCC 2 cut(s) 136, 696
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 6 cut(s) 174, 175, 192, 193, 403, 879
BmsI GCATC 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 371
BpuEI CTTGAG 2 cut(s) 73, 845
BpuMI CCSGG 4 cut(s) 174, 175, 192, 193
BsaJI CCNNGG 3 cut(s) 173, 191, 516
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 6 cut(s) 40, 70, 344, 374, 377, 407
Bsc4I CCNNNNNNNGG 5 cut(s) 42, 158, 220, 517, 522
Bse118I RCCGGY 1 cut(s) 310
Bse1I ACTGG 2 cut(s) 123, 354
BseAI TCCGGA 1 cut(s) 756
BseBI CCWGG 2 cut(s) 403, 879
BseDI CCNNGG 3 cut(s) 173, 191, 516
BseLI CCNNNNNNNGG 5 cut(s) 42, 158, 220, 517, 522
BseMII CTCAG 3 cut(s) 591, 659, 683
BseNI ACTGG 2 cut(s) 123, 354
BseRI GAGGAG 2 cut(s) 15, 76
BseXI GCAGC 3 cut(s) 3, 476, 622
BsgI GTGCAG 2 cut(s) 401, 829
BshNI GGYRCC 1 cut(s) 271
BsiHKCI CYCGRG 3 cut(s) 173, 191, 480
BsiSI CCGG 5 cut(s) 43, 174, 192, 311, 757
BslI CCNNNNNNNGG 5 cut(s) 42, 158, 220, 517, 522
BsmAI GTCTC 1 cut(s) 566
BsmBI CGTCTC 1 cut(s) 566
BsmI GAATGC 1 cut(s) 303
BsoBI CYCGRG 3 cut(s) 173, 191, 480
Bsp13I TCCGGA 1 cut(s) 756
Bsp143I GATC 3 cut(s) 316, 424, 804
BspCNI CTCAG 3 cut(s) 592, 658, 682
BspEI TCCGGA 1 cut(s) 756
BspLI GGNNCC 1 cut(s) 273
BspPI GGATC 2 cut(s) 324, 812
BspT107I GGYRCC 1 cut(s) 271
BsrBI CCGCTC 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 310
BsrI ACTGG 2 cut(s) 123, 354
BssAI RCCGGY 1 cut(s) 310
BssECI CCNNGG 3 cut(s) 173, 191, 516
BssMI GATC 3 cut(s) 316, 424, 804
BssT1I CCWWGG 1 cut(s) 516
Bst2UI CCWGG 2 cut(s) 403, 879
Bst4CI ACNGT 3 cut(s) 251, 474, 815
Bst6I CTCTTC 2 cut(s) 564, 624
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 6 cut(s) 237, 506, 600, 645, 669, 800
BstENI CCTNNNNNAGG 1 cut(s) 520
BstKTI GATC 3 cut(s) 319, 427, 807
BstMAI GTCTC 1 cut(s) 566
BstMBI GATC 3 cut(s) 316, 424, 804
BstMWI GCNNNNNNNGC 4 cut(s) 207, 227, 507, 749
BstNI CCWGG 2 cut(s) 403, 879
BstSCI CCNGG 6 cut(s) 172, 173, 190, 191, 401, 877
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 3, 476, 622
BstX2I RGATCY 1 cut(s) 804
BstYI RGATCY 1 cut(s) 804
BtsIMutI CAGTG 2 cut(s) 130, 330
Cac8I GCNNGC 1 cut(s) 512
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 136, 696
Cfr9I CCCGGG 2 cut(s) 173, 191
CpoI CGGWCCG 1 cut(s) 136
CseI GACGC 2 cut(s) 242, 583
Csp6I GTAC 1 cut(s) 361
CspI CGGWCCG 1 cut(s) 136
CviQI GTAC 1 cut(s) 361
DdeI CTNAG 6 cut(s) 237, 506, 600, 645, 669, 800
DpnI GATC 3 cut(s) 318, 426, 806
DpnII GATC 3 cut(s) 316, 424, 804
Eam1104I CTCTTC 2 cut(s) 564, 624
EarI CTCTTC 2 cut(s) 564, 624
EciI GGCGGA 3 cut(s) 22, 174, 236
Eco130I CCWWGG 1 cut(s) 516
Eco47I GGWCC 2 cut(s) 136, 696
Eco57I CTGAAG 1 cut(s) 560
Eco88I CYCGRG 3 cut(s) 173, 191, 480
EcoNI CCTNNNNNAGG 1 cut(s) 520
EcoRI GAATTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 401, 877
EcoT14I CCWWGG 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 516
Esp3I CGTCTC 1 cut(s) 566
FaiI YATR 6 cut(s) 396, 435, 462, 496, 774, 818
FalI AAGNNNNNCTT 2 cut(s) 561, 593
FauI CCCGC 1 cut(s) 115
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 5 cut(s) 17, 142, 465, 636, 727
Fsp4HI GCNGC 5 cut(s) 17, 142, 465, 636, 727
GluI GCNGC 5 cut(s) 17, 142, 465, 636, 727
GsuI CTGGAG 1 cut(s) 371
HapII CCGG 5 cut(s) 43, 174, 192, 311, 757
HgaI GACGC 2 cut(s) 242, 583
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 542
HinfI GANTC 4 cut(s) 178, 584, 641, 661
HpaII CCGG 5 cut(s) 43, 174, 192, 311, 757
HphI GGTGA 2 cut(s) 67, 496
Hpy166II GTNNAC 3 cut(s) 6, 376, 556
Hpy188I TCNGA 8 cut(s) 60, 339, 445, 540, 583, 589, 672, 700
Hpy188III TCNNGA 3 cut(s) 90, 757, 824
Hpy8I GTNNAC 3 cut(s) 6, 376, 556
Hpy99I CGWCG 2 cut(s) 10, 236
HpyAV CCTTC 2 cut(s) 88, 542
HpyCH4III ACNGT 3 cut(s) 251, 474, 815
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 5 cut(s) 303, 418, 464, 494, 846
HpyF10VI GCNNNNNNNGC 4 cut(s) 207, 227, 507, 749
HpyF3I CTNAG 6 cut(s) 237, 506, 600, 645, 669, 800
HpySE526I ACGT 1 cut(s) 558
Kpn2I TCCGGA 1 cut(s) 756
Kzo9I GATC 3 cut(s) 316, 424, 804
LmnI GCTCC 1 cut(s) 215
Lsp1109I GCAGC 3 cut(s) 3, 476, 622
LweI GCATC 1 cut(s) 481
MaeII ACGT 1 cut(s) 558
MalI GATC 3 cut(s) 318, 426, 806
MbiI CCGCTC 1 cut(s) 68
MboI GATC 3 cut(s) 316, 424, 804
MboII GAAGA 5 cut(s) 22, 578, 581, 641, 871
MflI RGATCY 1 cut(s) 804
MluCI AATT 4 cut(s) 94, 186, 528, 886
MlyI GAGTC 1 cut(s) 635
MmeI TCCRAC 2 cut(s) 83, 723
MnlI CCTC 6 cut(s) 36, 97, 268, 379, 625, 733
Mph1103I ATGCAT 1 cut(s) 496
MroI TCCGGA 1 cut(s) 756
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 2 cut(s) 840, 885
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 5 cut(s) 43, 174, 192, 311, 757
MspR9I CCNGG 6 cut(s) 174, 175, 192, 193, 403, 879
Mva1269I GAATGC 1 cut(s) 303
MvaI CCWGG 2 cut(s) 403, 879
MwoI GCNNNNNNNGC 4 cut(s) 207, 227, 507, 749
NciI CCSGG 4 cut(s) 174, 175, 192, 193
NdeII GATC 3 cut(s) 316, 424, 804
NlaIV GGNNCC 1 cut(s) 273
NsiI ATGCAT 1 cut(s) 496
PctI GAATGC 1 cut(s) 303
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 3 cut(s) 178, 584, 661
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 5 cut(s) 18, 143, 466, 637, 728
PleI GAGTC 1 cut(s) 635
PpsI GAGTC 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 401, 877
PspGI CCWGG 2 cut(s) 401, 877
PspN4I GGNNCC 1 cut(s) 273
PspPI GGNCC 2 cut(s) 136, 696
PsuI RGATCY 1 cut(s) 804
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 1 cut(s) 362
RsaNI GTAC 1 cut(s) 361
Rsr2I CGGWCCG 1 cut(s) 136
RsrII CGGWCCG 1 cut(s) 136
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 2 cut(s) 840, 885
SatI GCNGC 5 cut(s) 17, 142, 465, 636, 727
Sau3AI GATC 3 cut(s) 316, 424, 804
Sau96I GGNCC 2 cut(s) 136, 696
SchI GAGTC 1 cut(s) 635
ScrFI CCNGG 6 cut(s) 174, 175, 192, 193, 403, 879
SfaNI GCATC 1 cut(s) 481
SfcI CTRYAG 1 cut(s) 250
SinI GGWCC 2 cut(s) 136, 696
SmaI CCCGGG 2 cut(s) 175, 193
SmlI CTYRAG 2 cut(s) 88, 824
SmoI CTYRAG 2 cut(s) 88, 824
Sse9I AATT 4 cut(s) 94, 186, 528, 886
StyD4I CCNGG 6 cut(s) 172, 173, 190, 191, 401, 877
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 3 cut(s) 251, 474, 815
TaiI ACGT 1 cut(s) 561
TaqI TCGA 1 cut(s) 5
TasI AATT 4 cut(s) 94, 186, 528, 886
TauI GCSGC 2 cut(s) 144, 729
TfiI GAWTC 3 cut(s) 178, 584, 661
Tru1I TTAA 2 cut(s) 840, 885
Tru9I TTAA 2 cut(s) 840, 885
TscAI CASTG 2 cut(s) 130, 330
TseI GCWGC 3 cut(s) 16, 464, 635
TspDTI ATGAA 4 cut(s) 422, 579, 689, 801
TspMI CCCGGG 2 cut(s) 173, 191
TspRI CASTG 2 cut(s) 130, 330
Van91I CCANNNNNTGG 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 136, 696
XagI CCTNNNNNAGG 1 cut(s) 520
XapI RAATTY 2 cut(s) 94, 186
XmaI CCCGGG 2 cut(s) 173, 191
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 1 cut(s) 200
Zsp2I ATGCAT 1 cut(s) 496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.