AT5G11860

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004206
Physical Location & Seq
Reverse (-)
3821733 .. 3824298
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G11860.2

Sequence Viewer

Length: 918 bp
ATGGAAGATGATGACTCTCCTTCTTCTCGGGATTTGGATGCACAGAACCCTTATGATCGATTGCTTGCTCTAGATACAAGTACCGTAGATCCAAATTGTAACTTGGATTCGGTTTCTGCCATTTATCTAGCGATGAAGAGCTCTAAGCTGGAATGTGTTGACGAGCGTGGTCAAGATTCCCTTATTACTTCGGTATGCATGGAGGATGAAGAGGATGAGGAGCTCGATGAGTTTGATCCTTATCTGTTTATCAAGAACTTGCCGAACCTGTCTTCTGTTGTCCCAACTTTCAGGCCGGTCTTGCTTCCTAAACAAACCCGAAGCTGTCCTCCTATTTCTCTTGTCCTAGACCTTGATGAAACTCTTGTGCACTCGACTCTAGAGCCGTGTGGTGAGGTAGATTTCACATTCCCAGTGAATTTTAATGAAGAAGAGCATATGGTGTATGTGCGATGCCGTCCTCACCTCAAAGAGTTTATGGAGAGAGTGTCCCGACTTTTTGAGATCATTATATTTACAGCTAGCCAAAGTATTTATGCTGAGCAACTTCTGAACGTGCTTGACCCTAAGAGAAAGCTCTTTCGCCATAGAGTGTACCGTGACTCGTGTGTTTTCTTTGATGGTAACTACCTCAAGGATTTGTCAGTCCTCGGGCGTGATTTATCTCGTGTTATCATTGTTGATAACTCCCCACAGGCATTTGGTTTCCAAGTGGAAAATGGAGTGCCAATAGAGAGCTGGTTTAATGACCCATCAGATAAAGAACTCCTTCACTTGCTGCCATTTCTTGAAAGCCTAATTGGAGTTGAAGATGTAAGGCCAATGATCGCCAAGAAATTCAATCTAAGGGAGAAGATTGATGCAGCTGTAGCCGCACCTGAATATCCTGCTGAGGCGGGAGATCCTTTCGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

305

Amino Acids

34.85

Weight (kDa)

4.46

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 113 - 273 5.9e-61 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 873, 896
AclWI GGATC 3 cut(s) 83, 230, 896
AcsI RAATTY 2 cut(s) 418, 836
AfaI GTAC 2 cut(s) 82, 596
AgsI TTSAA 3 cut(s) 791, 809, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 783, 815
AluBI AGCT 8 cut(s) 141, 148, 223, 324, 521, 577, 738, 866
AluI AGCT 8 cut(s) 141, 148, 223, 324, 521, 577, 738, 866
Alw21I GWGCWC 3 cut(s) 143, 225, 372
Alw44I GTGCAC 1 cut(s) 368
AlwI GGATC 3 cut(s) 83, 230, 896
Ama87I CYCGRG 2 cut(s) 27, 650
AoxI GGCC 2 cut(s) 293, 818
ApaLI GTGCAC 1 cut(s) 368
ApeKI GCWGC 2 cut(s) 778, 863
ApoI RAATTY 2 cut(s) 418, 836
Asp700I GAANNNNTTC 1 cut(s) 768
AsuHPI GGTGA 2 cut(s) 404, 455
AsuII TTCGAA 1 cut(s) 909
AsuNHI GCTAGC 1 cut(s) 521
AvaI CYCGRG 2 cut(s) 27, 650
BaeGI GKGCMC 1 cut(s) 372
BanII GRGCYC 2 cut(s) 143, 225
BauI CACGAG 2 cut(s) 604, 666
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 3 cut(s) 143, 225, 372
BbvCI CCTCAGC 1 cut(s) 891
BbvI GCAGC 2 cut(s) 765, 875
BccI CCATC 2 cut(s) 614, 760
BceAI ACGGC 2 cut(s) 370, 441
BfaI CTAG 5 cut(s) 71, 128, 347, 380, 522
BfmI CTRYAG 1 cut(s) 867
BisI GCNGC 3 cut(s) 779, 864, 873
BlpI GCTNAGC 1 cut(s) 540
BlsI GCNGC 3 cut(s) 780, 865, 874
BmeT110I CYCGRG 2 cut(s) 27, 650
BmrI ACTGGG 1 cut(s) 407
BmsI GCATC 3 cut(s) 28, 443, 850
BmtI GCTAGC 1 cut(s) 525
BmuI ACTGGG 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 891
Bpu1102I GCTNAGC 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 617
Bsa29I ATCGAT 1 cut(s) 58
BsaBI GATNNNNATC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 649
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bse118I RCCGGY 1 cut(s) 295
Bse1I ACTGG 1 cut(s) 413
Bse8I GATNNNNATC 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 58
BseDI CCNNGG 1 cut(s) 649
BseGI GGATG 3 cut(s) 43, 211, 220
BseJI GATNNNNATC 1 cut(s) 240
BseMII CTCAG 2 cut(s) 531, 882
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 2 cut(s) 765, 875
BshFI GGCC 2 cut(s) 295, 820
BshVI ATCGAT 1 cut(s) 58
BsiHKAI GWGCWC 3 cut(s) 143, 225, 372
BsiHKCI CYCGRG 2 cut(s) 27, 650
BsiSI CCGG 1 cut(s) 296
BslFI GGGAC 2 cut(s) 266, 475
BsmFI GGGAC 2 cut(s) 266, 475
BsnI GGCC 2 cut(s) 295, 820
BsoBI CYCGRG 2 cut(s) 27, 650
Bsp119I TTCGAA 1 cut(s) 909
Bsp1286I GDGCHC 3 cut(s) 143, 225, 372
Bsp143I GATC 6 cut(s) 55, 88, 235, 504, 825, 901
Bsp1720I GCTNAGC 1 cut(s) 540
BspACI CCGC 2 cut(s) 873, 896
BspANI GGCC 2 cut(s) 295, 820
BspCNI CTCAG 2 cut(s) 532, 883
BspDI ATCGAT 1 cut(s) 58
BspOI GCTAGC 1 cut(s) 525
BspPI GGATC 3 cut(s) 83, 230, 896
BspQI GCTCTTC 2 cut(s) 131, 426
BspT104I TTCGAA 1 cut(s) 909
BsrFI RCCGGY 1 cut(s) 295
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 295
BssECI CCNNGG 1 cut(s) 649
BssMI GATC 6 cut(s) 55, 88, 235, 504, 825, 901
BssSI CACGAG 2 cut(s) 604, 666
Bst2BI CACGAG 2 cut(s) 604, 666
Bst4CI ACNGT 2 cut(s) 85, 599
Bst6I CTCTTC 3 cut(s) 131, 204, 426
BstBI TTCGAA 1 cut(s) 909
BstC8I GCNNGC 2 cut(s) 66, 523
BstDEI CTNAG 5 cut(s) 144, 540, 567, 845, 891
BstF5I GGATG 3 cut(s) 43, 211, 220
BstKTI GATC 6 cut(s) 58, 91, 238, 507, 828, 904
BstMBI GATC 6 cut(s) 55, 88, 235, 504, 825, 901
BstMWI GCNNNNNNNGC 3 cut(s) 301, 869, 872
BstSFI CTRYAG 1 cut(s) 867
BstSLI GKGCMC 1 cut(s) 372
BstV1I GCAGC 2 cut(s) 765, 875
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 2 cut(s) 88, 901
BstYI RGATCY 2 cut(s) 88, 901
Bsu15I ATCGAT 1 cut(s) 58
BsuRI GGCC 2 cut(s) 295, 820
BsuTUI ATCGAT 1 cut(s) 58
BtgZI GCGATG 2 cut(s) 146, 466
BtsCI GGATG 3 cut(s) 43, 211, 220
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 66, 523
Cfr10I RCCGGY 1 cut(s) 295
ClaI ATCGAT 1 cut(s) 58
Csp6I GTAC 2 cut(s) 81, 595
CviAII CATG 1 cut(s) 199
CviQI GTAC 2 cut(s) 81, 595
DdeI CTNAG 5 cut(s) 144, 540, 567, 845, 891
DpnI GATC 6 cut(s) 57, 90, 237, 506, 827, 903
DpnII GATC 6 cut(s) 55, 88, 235, 504, 825, 901
Eam1104I CTCTTC 3 cut(s) 131, 204, 426
EarI CTCTTC 3 cut(s) 131, 204, 426
Ecl136II GAGCTC 2 cut(s) 141, 223
Eco24I GRGCYC 2 cut(s) 143, 225
Eco53kI GAGCTC 2 cut(s) 141, 223
Eco88I CYCGRG 2 cut(s) 27, 650
EcoICRI GAGCTC 2 cut(s) 141, 223
EcoT22I ATGCAT 1 cut(s) 200
EcoT38I GRGCYC 2 cut(s) 143, 225
FaeI CATG 1 cut(s) 202
FalI AAGNNNNNCTT 4 cut(s) 165, 197, 753, 785
FaqI GGGAC 2 cut(s) 266, 475
FatI CATG 1 cut(s) 198
FauI CCCGC 1 cut(s) 889
FauNDI CATATG 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 779, 864, 873
FokI GGATG 3 cut(s) 50, 218, 227
FriOI GRGCYC 2 cut(s) 143, 225
Fsp4HI GCNGC 3 cut(s) 779, 864, 873
FspBI CTAG 5 cut(s) 71, 128, 347, 380, 522
GluI GCNGC 3 cut(s) 779, 864, 873
HaeIII GGCC 2 cut(s) 295, 820
HapII CCGG 1 cut(s) 296
Hin1II CATG 1 cut(s) 202
HincII GTYRAC 1 cut(s) 160
HindII GTYRAC 1 cut(s) 160
HinfI GANTC 5 cut(s) 14, 107, 176, 376, 602
HpaII CCGG 1 cut(s) 296
HphI GGTGA 2 cut(s) 404, 455
Hpy166II GTNNAC 3 cut(s) 160, 370, 595
Hpy188I TCNGA 2 cut(s) 552, 757
Hpy188III TCNNGA 7 cut(s) 29, 71, 173, 253, 380, 492, 788
Hpy8I GTNNAC 3 cut(s) 160, 370, 595
HpyAV CCTTC 2 cut(s) 30, 779
HpyCH4III ACNGT 2 cut(s) 85, 599
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 4 cut(s) 41, 198, 370, 863
HpyF10VI GCNNNNNNNGC 3 cut(s) 301, 869, 872
HpyF3I CTNAG 5 cut(s) 144, 540, 567, 845, 891
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 1 cut(s) 202
Kzo9I GATC 6 cut(s) 55, 88, 235, 504, 825, 901
LguI GCTCTTC 2 cut(s) 131, 426
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 9 cut(s) 134, 277, 281, 309, 426, 680, 724, 891, 900
Lsp1109I GCAGC 2 cut(s) 765, 875
LweI GCATC 3 cut(s) 28, 443, 850
MaeI CTAG 5 cut(s) 71, 128, 347, 380, 522
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 3 cut(s) 98, 599, 623
MalI GATC 6 cut(s) 57, 90, 237, 506, 827, 903
MboI GATC 6 cut(s) 55, 88, 235, 504, 825, 901
MboII GAAGA 9 cut(s) 15, 17, 148, 221, 264, 440, 443, 821, 865
MflI RGATCY 2 cut(s) 88, 901
MhlI GDGCHC 3 cut(s) 143, 225, 372
MluCI AATT 4 cut(s) 94, 418, 798, 836
MlyI GAGTC 3 cut(s) 8, 370, 596
Mph1103I ATGCAT 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 768
MseI TTAA 2 cut(s) 423, 744
MspA1I CMGCKG 1 cut(s) 866
MspI CCGG 1 cut(s) 296
MwoI GCNNNNNNNGC 3 cut(s) 301, 869, 872
NdeI CATATG 1 cut(s) 438
NdeII GATC 6 cut(s) 55, 88, 235, 504, 825, 901
NheI GCTAGC 1 cut(s) 521
NlaIII CATG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 599
NsiI ATGCAT 1 cut(s) 200
NspV TTCGAA 1 cut(s) 909
PciSI GCTCTTC 2 cut(s) 131, 426
PdmI GAANNNNTTC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 107, 176
PkrI GCNGC 3 cut(s) 780, 865, 874
PleI GAGTC 3 cut(s) 8, 370, 596
PpsI GAGTC 3 cut(s) 8, 370, 596
Psp124BI GAGCTC 2 cut(s) 143, 225
PsuI RGATCY 2 cut(s) 88, 901
PvuII CAGCTG 1 cut(s) 866
RsaI GTAC 2 cut(s) 82, 596
RsaNI GTAC 2 cut(s) 81, 595
SacI GAGCTC 2 cut(s) 143, 225
SapI GCTCTTC 2 cut(s) 131, 426
SaqAI TTAA 2 cut(s) 423, 744
SatI GCNGC 3 cut(s) 779, 864, 873
Sau3AI GATC 6 cut(s) 55, 88, 235, 504, 825, 901
SchI GAGTC 3 cut(s) 8, 370, 596
SduI GDGCHC 3 cut(s) 143, 225, 372
SfaNI GCATC 3 cut(s) 28, 443, 850
SfcI CTRYAG 1 cut(s) 867
SfuI TTCGAA 1 cut(s) 909
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 4 cut(s) 94, 418, 798, 836
SsiI CCGC 2 cut(s) 873, 896
SspMI CTAG 5 cut(s) 71, 128, 347, 380, 522
SstI GAGCTC 2 cut(s) 143, 225
TaaI ACNGT 2 cut(s) 85, 599
TaiI ACGT 1 cut(s) 558
TaqI TCGA 4 cut(s) 58, 225, 374, 909
TasI AATT 4 cut(s) 94, 418, 798, 836
TauI GCSGC 1 cut(s) 875
TfiI GAWTC 2 cut(s) 107, 176
Tru1I TTAA 2 cut(s) 423, 744
Tru9I TTAA 2 cut(s) 423, 744
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 599
TseI GCWGC 2 cut(s) 778, 863
Tsp45I GTSAC 1 cut(s) 599
TspDTI ATGAA 4 cut(s) 149, 222, 372, 441
TspRI CASTG 1 cut(s) 420
VneI GTGCAC 1 cut(s) 368
XapI RAATTY 2 cut(s) 418, 836
XbaI TCTAGA 2 cut(s) 70, 379
XcmI CCANNNNNNNNNTGG 2 cut(s) 716, 735
XmnI GAANNNNTTC 1 cut(s) 768
XspI CTAG 5 cut(s) 71, 128, 347, 380, 522
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.