RchiOBHm_Chr4g0430361

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0004206
Physical Location & Seq
Reverse (-)
54906123 .. 54911558
5436 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39908

Sequence Viewer

Length: 1362 bp
ATGCAAACAAGGAAAAAAAGTTCTGGAAGAACTGCTTCTAGAGAGCATACCAGTCCCAAGGTTACAAGAGCTCAGAAGAAAACACATGAAAATGTCAAAGTATTGGATACCAAAGTTACAGATATGATTACATCTTCATCCAGAAAGCAGAGACTCGGTGGGACTCATAAGAAGAATGAGGAGCCTGTTACTGCAAGCACGTTAAGTACAAATTATGACTTGCTGCTTAATGAGACTGCCGAGGCTTGTTTGGGGCATGATGCTTTAAGTGATGATTGCAAAGAGAATAAGGGTTGTGAGGAGGGTGCTGCTGGTTGCGTTCTGGGAACTATATTTTCTCCTGCCTTTCACATACCGAAACATGCTGGTGGAGAATCTGCCAATGGAGTTGATTTTGTTAAATTCTTTCAAAATGGAGATCACAATTTCCATAAAGAGATGGAAATAAAGTACTCACCCGATGATGCACACCATGGTTATGTTGGCCAAGAGACATCTGAGTCCACTGTTCGAGACGAGAACAACTACTTTGTGGAGGCAACCAATATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCCAAGTTGGAATGTTTAGATGAGCATGGTCAGGAACCTATGTCAACTGATTTGTACGTGGATGATGATGAGAGCGAGGAATTTGATGACTTTGATCCTTACTTATTTATCAAGAACTTACCTGACTTGTCCTCAGTTGTCCCCACTTTTCGGCCGTTGCTGCTGCCTAAGCAGACTCGAAGTTGTCCTCCTACAAGTCTTGTTTTGGACTTGGATGAAACTCTGGTACATTCCACACTAGAACCTTGTGATGATGCAGACTTCACATTTCCTGTAAATTTTAACCTACAAGAGCATACAGTATATGTTCGATGCCGTCCTCACCTCAGAGATTTTCTGGACAGAGTTTCGCTTCTTTTCGAGATCATTATCTTCACAGCTAGTCAAAGTATCTATGCAGAGCAGCTTCTAAATGTGCTGGATCCGAAGAGGAAGATTTTTCGCCATCGTGTTTTTCGTGATTCCTGTGTTTTTGTGGAGGGGAATTATCTCAAAGATTTGTCGGTTCTTGGCCGTGATTTGGCACGTGTTGTCATAGTCGACAACTCTCCACAGGCATTTGGTTTCCAAGTGGACAATGGAATACCTATTGAGAGCTGGTTTGATGATCGTTCTGATACAGAATTACTTTTGTTACTTCCTTTTTTGGAAAGCTTGGTTGGAGTAGAAGATGTTCGACCGCTAATTGCAAACAAATTCAACCTCCGGGAGAAAATAGCTACTGCTGATTATCCACTAAACTCAAATAGAGGTGATTCTTTAGTGCCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

453

Amino Acids

51.02

Weight (kDa)

5.0

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 263 - 422 3.3e-59 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
AccI GTMKAC 1 cut(s) 1128
AciI CCGC 1 cut(s) 1268
AclWI GGATC 3 cut(s) 677, 1004, 1017
AcoI YGGCCR 3 cut(s) 484, 740, 1099
AcsI RAATTY 5 cut(s) 401, 586, 668, 865, 1283
AcuI CTGAAG 1 cut(s) 576
AcvI CACGTG 1 cut(s) 1115
AfaI GTAC 4 cut(s) 208, 452, 644, 816
AfiI CCNNNNNNNGG 2 cut(s) 738, 1108
AflIII ACRYGT 1 cut(s) 1114
AgsI TTSAA 2 cut(s) 410, 1288
AjuI GAANNNNNNNTTGG 2 cut(s) 1230, 1262
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 6 cut(s) 71, 968, 994, 1185, 1242, 1307
AluI AGCT 6 cut(s) 71, 968, 994, 1185, 1242, 1307
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 5 cut(s) 145, 227, 485, 507, 565
AlwI GGATC 3 cut(s) 677, 1004, 1017
AoxI GGCC 3 cut(s) 484, 740, 1099
ApeKI GCWGC 5 cut(s) 223, 308, 748, 751, 991
ApoI RAATTY 5 cut(s) 401, 586, 668, 865, 1283
Asp700I GAANNNNTTC 2 cut(s) 34, 1260
AsuC2I CCSGG 1 cut(s) 1295
AsuHPI GGTGA 3 cut(s) 447, 902, 1352
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BalI TGGCCA 1 cut(s) 486
BamHI GGATCC 1 cut(s) 1009
BanII GRGCYC 1 cut(s) 73
BbrPI CACGTG 1 cut(s) 1115
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 5 cut(s) 210, 295, 735, 738, 1003
BccI CCATC 2 cut(s) 433, 1041
BceAI ACGGC 3 cut(s) 727, 888, 1086
BciVI GTATCC 1 cut(s) 100
BcnI CCSGG 1 cut(s) 1295
BcoDI GTCTC 5 cut(s) 145, 227, 485, 507, 565
BfaI CTAG 3 cut(s) 39, 827, 969
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 5 cut(s) 224, 309, 749, 752, 992
BlsI GCNGC 5 cut(s) 225, 310, 750, 753, 993
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 1 cut(s) 1295
BmiI GGNNCC 3 cut(s) 183, 624, 1011
BmrFI CCNGG 1 cut(s) 1295
BmsI GCATC 4 cut(s) 250, 454, 832, 890
Bpu10I CCTNAGC 1 cut(s) 756
BpuMI CCSGG 1 cut(s) 1295
BsaAI YACGTR 2 cut(s) 646, 1115
BsaBI GATNNNNATC 1 cut(s) 956
BsaJI CCNNGG 3 cut(s) 57, 240, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 738, 1108
Bse1I ACTGG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 956
BseDI CCNNGG 3 cut(s) 57, 240, 472
BseGI GGATG 3 cut(s) 137, 655, 808
BseJI GATNNNNATC 1 cut(s) 956
BseLI CCNNNNNNNGG 2 cut(s) 738, 1108
BseMII CTCAG 5 cut(s) 86, 489, 576, 735, 928
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 2 cut(s) 194, 314
BseX3I CGGCCG 1 cut(s) 740
BseXI GCAGC 5 cut(s) 210, 295, 735, 738, 1003
Bsh1285I CGRYCG 2 cut(s) 743, 1268
BshFI GGCC 3 cut(s) 486, 742, 1101
BsiEI CGRYCG 2 cut(s) 743, 1268
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 1294
BslFI GGGAC 3 cut(s) 39, 175, 713
BslI CCNNNNNNNGG 2 cut(s) 738, 1108
BsmAI GTCTC 5 cut(s) 145, 227, 485, 507, 565
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 3 cut(s) 39, 175, 713
BsnI GGCC 3 cut(s) 486, 742, 1101
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 5 cut(s) 418, 682, 951, 1009, 1195
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 1 cut(s) 1268
BspANI GGCC 3 cut(s) 486, 742, 1101
BspCNI CTCAG 5 cut(s) 85, 490, 575, 734, 927
BspLI GGNNCC 3 cut(s) 183, 624, 1011
BspPI GGATC 3 cut(s) 677, 1004, 1017
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 57, 240, 472
BssMI GATC 5 cut(s) 418, 682, 951, 1009, 1195
BssT1I CCWWGG 2 cut(s) 57, 472
Bst4CI ACNGT 2 cut(s) 508, 889
Bst6I CTCTTC 1 cut(s) 1010
BstBAI YACGTR 2 cut(s) 646, 1115
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 6 cut(s) 72, 498, 562, 721, 756, 914
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 3 cut(s) 137, 655, 808
BstKTI GATC 5 cut(s) 421, 685, 954, 1012, 1198
BstMAI GTCTC 5 cut(s) 145, 227, 485, 507, 565
BstMBI GATC 5 cut(s) 418, 682, 951, 1009, 1195
BstMCI CGRYCG 2 cut(s) 743, 1268
BstMWI GCNNNNNNNGC 2 cut(s) 748, 757
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 1293
BstV1I GCAGC 5 cut(s) 210, 295, 735, 738, 1003
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BstZI CGGCCG 1 cut(s) 740
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 3 cut(s) 486, 742, 1101
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 3 cut(s) 137, 655, 808
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 196
Csp6I GTAC 4 cut(s) 207, 451, 643, 815
CviAII CATG 6 cut(s) 86, 257, 362, 473, 580, 614
CviQI GTAC 4 cut(s) 207, 451, 643, 815
DdeI CTNAG 6 cut(s) 72, 498, 562, 721, 756, 914
DpnI GATC 5 cut(s) 420, 684, 953, 1011, 1197
DpnII GATC 5 cut(s) 418, 682, 951, 1009, 1195
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 3 cut(s) 484, 740, 1099
EagI CGGCCG 1 cut(s) 740
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 740
Eco130I CCWWGG 2 cut(s) 57, 472
Eco24I GRGCYC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 740
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 576
Eco72I CACGTG 1 cut(s) 1115
EcoICRI GAGCTC 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 57, 472
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 57, 472
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 6 cut(s) 89, 260, 365, 476, 583, 617
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 3 cut(s) 39, 175, 713
FatI CATG 6 cut(s) 85, 256, 361, 472, 579, 613
FblI GTMKAC 1 cut(s) 1128
Fnu4HI GCNGC 5 cut(s) 224, 309, 749, 752, 992
FokI GGATG 3 cut(s) 124, 662, 815
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 5 cut(s) 224, 309, 749, 752, 992
FspBI CTAG 3 cut(s) 39, 827, 969
GluI GCNGC 5 cut(s) 224, 309, 749, 752, 992
HaeIII GGCC 3 cut(s) 486, 742, 1101
HapII CCGG 1 cut(s) 1294
Hin1II CATG 6 cut(s) 89, 260, 365, 476, 583, 617
HincII GTYRAC 2 cut(s) 633, 1129
HindII GTYRAC 2 cut(s) 633, 1129
HindIII AAGCTT 1 cut(s) 1240
HinfI GANTC 7 cut(s) 153, 163, 374, 500, 763, 1049, 1343
HpaII CCGG 1 cut(s) 1294
HphI GGTGA 3 cut(s) 447, 902, 1352
Hpy166II GTNNAC 4 cut(s) 504, 633, 1129, 1162
Hpy188I TCNGA 6 cut(s) 75, 499, 556, 917, 1014, 1204
Hpy188III TCNNGA 9 cut(s) 24, 39, 141, 512, 620, 700, 926, 949, 1046
Hpy8I GTNNAC 4 cut(s) 504, 633, 1129, 1162
HpyCH4III ACNGT 2 cut(s) 508, 889
HpyCH4IV ACGT 3 cut(s) 200, 645, 1114
HpyCH4V TGCA 7 cut(s) 4, 194, 279, 467, 845, 986, 1277
HpyF10VI GCNNNNNNNGC 2 cut(s) 748, 757
HpyF3I CTNAG 6 cut(s) 72, 498, 562, 721, 756, 914
HpySE526I ACGT 3 cut(s) 200, 645, 1114
Hsp92II CATG 6 cut(s) 89, 260, 365, 476, 583, 617
Kzo9I GATC 5 cut(s) 418, 682, 951, 1009, 1195
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 5 cut(s) 210, 295, 735, 738, 1003
LweI GCATC 4 cut(s) 250, 454, 832, 890
MaeI CTAG 3 cut(s) 39, 827, 969
MaeII ACGT 3 cut(s) 200, 645, 1114
MaeIII GTNAC 4 cut(s) 61, 115, 187, 1221
MalI GATC 5 cut(s) 420, 684, 953, 1011, 1197
MboI GATC 5 cut(s) 418, 682, 951, 1009, 1195
MboII GAAGA 9 cut(s) 39, 88, 126, 184, 543, 952, 1027, 1033, 1268
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 4 cut(s) 147, 157, 509, 757
MmeI TCCRAC 2 cut(s) 576, 1228
Mox20I TGGCCA 1 cut(s) 486
MroXI GAANNNNTTC 2 cut(s) 34, 1260
MscI TGGCCA 1 cut(s) 486
MseI TTAA 5 cut(s) 203, 228, 266, 399, 870
MslI CAYNNNNRTG 3 cut(s) 90, 366, 477
Msp20I TGGCCA 1 cut(s) 486
MspI CCGG 1 cut(s) 1294
MspR9I CCNGG 1 cut(s) 1295
MwoI GCNNNNNNNGC 2 cut(s) 748, 757
NciI CCSGG 1 cut(s) 1295
NcoI CCATGG 1 cut(s) 472
NdeII GATC 5 cut(s) 418, 682, 951, 1009, 1195
NlaIII CATG 6 cut(s) 89, 260, 365, 476, 583, 617
NlaIV GGNNCC 3 cut(s) 183, 624, 1011
NmeAIII GCCGAG 1 cut(s) 265
NspI RCATGY 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 1042
PdmI GAANNNNTTC 2 cut(s) 34, 1260
PfeI GAWTC 3 cut(s) 374, 1049, 1343
PfoI TCCNGGA 1 cut(s) 1293
PkrI GCNGC 5 cut(s) 225, 310, 750, 753, 993
PleI GAGTC 4 cut(s) 147, 157, 508, 757
PmaCI CACGTG 1 cut(s) 1115
PmlI CACGTG 1 cut(s) 1115
PpsI GAGTC 4 cut(s) 147, 157, 508, 757
Ppu21I YACGTR 2 cut(s) 646, 1115
Psp124BI GAGCTC 1 cut(s) 73
PspCI CACGTG 1 cut(s) 1115
PspN4I GGNNCC 3 cut(s) 183, 624, 1011
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PsuI RGATCY 1 cut(s) 1009
RsaI GTAC 4 cut(s) 208, 452, 644, 816
RsaNI GTAC 4 cut(s) 207, 451, 643, 815
RseI CAYNNNNRTG 3 cut(s) 90, 366, 477
SacI GAGCTC 1 cut(s) 73
SalI GTCGAC 1 cut(s) 1127
SaqAI TTAA 5 cut(s) 203, 228, 266, 399, 870
SatI GCNGC 5 cut(s) 224, 309, 749, 752, 992
Sau3AI GATC 5 cut(s) 418, 682, 951, 1009, 1195
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 4 cut(s) 147, 157, 509, 757
ScrFI CCNGG 1 cut(s) 1295
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 4 cut(s) 250, 454, 832, 890
SmiMI CAYNNNNRTG 3 cut(s) 90, 366, 477
SsiI CCGC 1 cut(s) 1268
SspI AATATT 1 cut(s) 547
SspMI CTAG 3 cut(s) 39, 827, 969
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 1293
StyI CCWWGG 2 cut(s) 57, 472
TaaI ACNGT 2 cut(s) 508, 889
TaiI ACGT 3 cut(s) 203, 648, 1117
TaqI TCGA 6 cut(s) 511, 766, 898, 948, 1128, 1264
TatI WGTACW 2 cut(s) 206, 450
TfiI GAWTC 3 cut(s) 374, 1049, 1343
Tru1I TTAA 5 cut(s) 203, 228, 266, 399, 870
Tru9I TTAA 5 cut(s) 203, 228, 266, 399, 870
TscAI CASTG 1 cut(s) 511
TseI GCWGC 5 cut(s) 223, 308, 748, 751, 991
TspDTI ATGAA 4 cut(s) 102, 126, 596, 819
TspRI CASTG 1 cut(s) 511
XapI RAATTY 5 cut(s) 401, 586, 668, 865, 1283
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 2 cut(s) 479, 1163
XmiI GTMKAC 1 cut(s) 1128
XmnI GAANNNNTTC 2 cut(s) 34, 1260
XspI CTAG 3 cut(s) 39, 827, 969
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.