Rh4AG299000

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0004206
Physical Location & Seq
Reverse (-)
61209523 .. 61214988
5466 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG299000.1

Sequence Viewer

Length: 1395 bp
ATGCAAACAAGGAAAAAAAGTTCTGGAAGAACTGCTTCTAGAGAGCATACCAGTCCCAAGGTTACAAGAGCTCAGAAGAAAACACATGAAAATGTCAAAGTATTGGATACCAAAGTTACAGATATGATTACATCTTCCTCCAGGAAGCAGAGACTCGGTGGGACTCATAAGAAGAATGAGGAGCCTGTTACTGCAAGCACTTTAAGTACAAATTATGACTTGCTGCTTAACGAGACTGCCGAGGCTTGTTTGGGGCATGATGCTTTAAGTGATGATTGCAAAGAGAATAAGGGTTGTGAGGAGGGTACTACTGATTGCGAGCTGGGAACTATATTTTCTCCTGCCTTTCACATCCCGAAACATGCTGGTGGAGAATCTGCCAATGGAGTTGATTTTGTTAAATTCTTTCAAAATGGAGATCACAATTTCCATCAAGAGATGGAAATAAAGTACTCACCCGATGATGCACACCATGGTTATGTTGGCCAAGAGACATCCGAGTCCACTGTTCGAGACGAGAACAACTACTGTGAGTACTCATTATCTTTTGTAAATATAACATGTCCTGTTGTGGAGGCAACCAATATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCCAAGTTGGAATGTTTAGATGATCATGGTCAGGAACCTATGTCAACTGATTTGTACGTGGATGATGATGAGAGTGAGGAATTTGATGACTTTGATCCTTACTTATTTATCAAGAACTTACCTGACTTGTCCTCAGTTGTCCCCACTTTTCGGCCGTTGCCTAAACAGACTCGAAGTTGTCCTCCTACAAGTCTTGTTTTGGACTTGGATGAAACTCTGGTACATTCCACGCTAGAACCTTGTGATGATGCAGACTTCACATTTCCCATAAATTTTAACCTACAAGAGCATACAGTATATGTTCGATGCCGTCCTCATCTCAGAGATTTTCTAGACCGAGTTTCGCTTCTTTTCGAGATCATTATCTTCACAGCTAGTCAAAGTATCTATGCAGAGCAGCTTCTAAATGTGCTGGATCCGAAGAGGAAGATTTTTCGCCATCGTGTTTTTCGTGATTCCTGTGTTTTTGTGGGGGGGAATTATCTCAAAGATTTGTCCGTTCTTGGCCGTGATTTGGCACATGTTGTCATTGTCGACAACTCTCCACAGGCATTTGGGTTCCAAGTGGACAATGGAATACCAATTGAGAGCTGGTTTGATGATCGTTCTGATACAGAATTACTTTCATTACTTCCTTTTTTGGAAAGCTTGGTTGGAGTAGAAGATGTTCGACCGCTAATTGCAAACAAATTCAACCTCAGGGAGAAAGTAGCTAGTGCTGATTATCCGCTAAACTCAAATAGAGGTGATTCTTTAGTGCCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

464

Amino Acids

52.21

Weight (kDa)

4.89

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 274 - 433 5.1e-59 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
AccI GTMKAC 1 cut(s) 1161
AciI CCGC 2 cut(s) 1301, 1355
AclWI GGATC 3 cut(s) 716, 1037, 1050
AcoI YGGCCR 3 cut(s) 484, 779, 1132
AcsI RAATTY 5 cut(s) 401, 625, 707, 898, 1316
AcuI CTGAAG 1 cut(s) 615
AfaI GTAC 6 cut(s) 208, 307, 452, 536, 683, 849
AfiI CCNNNNNNNGG 2 cut(s) 777, 1141
AflIII ACRYGT 2 cut(s) 560, 1147
AgsI TTSAA 2 cut(s) 410, 1321
AjnI CCWGG 1 cut(s) 140
AjuI GAANNNNNNNTTGG 2 cut(s) 1263, 1295
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 7 cut(s) 71, 322, 1001, 1027, 1218, 1275, 1340
AluI AGCT 7 cut(s) 71, 322, 1001, 1027, 1218, 1275, 1340
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 5 cut(s) 145, 227, 485, 507, 604
AlwI GGATC 3 cut(s) 716, 1037, 1050
AoxI GGCC 3 cut(s) 484, 779, 1132
ApeKI GCWGC 2 cut(s) 223, 1024
ApoI RAATTY 5 cut(s) 401, 625, 707, 898, 1316
Asp700I GAANNNNTTC 2 cut(s) 34, 1293
AsuHPI GGTGA 2 cut(s) 447, 1385
AxyI CCTNAGG 1 cut(s) 1325
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BalI TGGCCA 1 cut(s) 486
BamHI GGATCC 1 cut(s) 1042
BanII GRGCYC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 210, 1036
BccI CCATC 3 cut(s) 433, 438, 1074
BceAI ACGGC 3 cut(s) 766, 921, 1119
BciT130I CCWGG 1 cut(s) 142
BciVI GTATCC 1 cut(s) 100
BclI TGATCA 1 cut(s) 649
BcoDI GTCTC 5 cut(s) 145, 227, 485, 507, 604
BfaI CTAG 5 cut(s) 39, 860, 959, 1002, 1341
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 2 cut(s) 224, 1025
BlsI GCNGC 2 cut(s) 225, 1026
BmcAI AGTACT 2 cut(s) 452, 536
Bme1390I CCNGG 1 cut(s) 142
BmiI GGNNCC 4 cut(s) 183, 663, 1044, 1187
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 4 cut(s) 250, 454, 865, 923
BpmI CTGGAG 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 685
BsaBI GATNNNNATC 1 cut(s) 989
BsaJI CCNNGG 3 cut(s) 57, 240, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 777, 1141
Bse1I ACTGG 1 cut(s) 51
Bse21I CCTNAGG 1 cut(s) 1325
Bse8I GATNNNNATC 1 cut(s) 989
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 3 cut(s) 57, 240, 472
BseGI GGATG 4 cut(s) 351, 494, 694, 841
BseJI GATNNNNATC 1 cut(s) 989
BseLI CCNNNNNNNGG 2 cut(s) 777, 1141
BseMII CTCAG 5 cut(s) 86, 615, 774, 961, 1339
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 2 cut(s) 194, 314
BseX3I CGGCCG 1 cut(s) 779
BseXI GCAGC 2 cut(s) 210, 1036
BseYI CCCAGC 1 cut(s) 322
Bsh1285I CGRYCG 2 cut(s) 782, 1301
BshFI GGCC 3 cut(s) 486, 781, 1134
BsiEI CGRYCG 2 cut(s) 782, 1301
BsiHKAI GWGCWC 1 cut(s) 73
BslFI GGGAC 3 cut(s) 39, 175, 752
BslI CCNNNNNNNGG 2 cut(s) 777, 1141
BsmAI GTCTC 5 cut(s) 145, 227, 485, 507, 604
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 3 cut(s) 39, 175, 752
BsnI GGCC 3 cut(s) 486, 781, 1134
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 2 cut(s) 1301, 1355
BspANI GGCC 3 cut(s) 486, 781, 1134
BspCNI CTCAG 5 cut(s) 85, 614, 773, 960, 1338
BspLI GGNNCC 4 cut(s) 183, 663, 1044, 1187
BspPI GGATC 3 cut(s) 716, 1037, 1050
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 57, 240, 472
BssMI GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
BssT1I CCWWGG 2 cut(s) 57, 472
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 3 cut(s) 508, 530, 922
Bst6I CTCTTC 1 cut(s) 1043
BstBAI YACGTR 1 cut(s) 685
BstC8I GCNNGC 2 cut(s) 196, 320
BstDEI CTNAG 5 cut(s) 72, 601, 760, 947, 1325
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 4 cut(s) 351, 494, 694, 841
BstKTI GATC 6 cut(s) 421, 652, 724, 987, 1045, 1231
BstMAI GTCTC 5 cut(s) 145, 227, 485, 507, 604
BstMBI GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
BstMCI CGRYCG 2 cut(s) 782, 1301
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 3 cut(s) 365, 564, 1151
BstSCI CCNGG 1 cut(s) 140
BstV1I GCAGC 2 cut(s) 210, 1036
BstX2I RGATCY 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 1042
BstZI CGGCCG 1 cut(s) 779
Bsu36I CCTNAGG 1 cut(s) 1325
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 3 cut(s) 486, 781, 1134
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 4 cut(s) 351, 494, 694, 841
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 196, 320
Csp6I GTAC 6 cut(s) 207, 306, 451, 535, 682, 848
CviAII CATG 8 cut(s) 86, 257, 362, 473, 561, 619, 653, 1148
CviQI GTAC 6 cut(s) 207, 306, 451, 535, 682, 848
DdeI CTNAG 5 cut(s) 72, 601, 760, 947, 1325
DpnI GATC 6 cut(s) 420, 651, 723, 986, 1044, 1230
DpnII GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 3 cut(s) 484, 779, 1132
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 1043
EarI CTCTTC 1 cut(s) 1043
Ecl136II GAGCTC 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 779
Eco130I CCWWGG 2 cut(s) 57, 472
Eco24I GRGCYC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 779
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 615
Eco81I CCTNAGG 1 cut(s) 1325
EcoICRI GAGCTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 2 cut(s) 57, 472
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 57, 472
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 8 cut(s) 89, 260, 365, 476, 564, 622, 656, 1151
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 3 cut(s) 39, 175, 752
FatI CATG 8 cut(s) 85, 256, 361, 472, 560, 618, 652, 1147
FbaI TGATCA 1 cut(s) 649
FblI GTMKAC 1 cut(s) 1161
Fnu4HI GCNGC 2 cut(s) 224, 1025
FokI GGATG 4 cut(s) 338, 481, 701, 848
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 2 cut(s) 224, 1025
FspBI CTAG 5 cut(s) 39, 860, 959, 1002, 1341
GluI GCNGC 2 cut(s) 224, 1025
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 3 cut(s) 486, 781, 1134
Hin1II CATG 8 cut(s) 89, 260, 365, 476, 564, 622, 656, 1151
HincII GTYRAC 2 cut(s) 672, 1162
HindII GTYRAC 2 cut(s) 672, 1162
HindIII AAGCTT 1 cut(s) 1273
HinfI GANTC 7 cut(s) 153, 163, 374, 500, 796, 1082, 1376
HphI GGTGA 2 cut(s) 447, 1385
Hpy166II GTNNAC 4 cut(s) 504, 672, 1162, 1195
Hpy188I TCNGA 6 cut(s) 75, 499, 595, 950, 1047, 1237
Hpy8I GTNNAC 4 cut(s) 504, 672, 1162, 1195
HpyCH4III ACNGT 3 cut(s) 508, 530, 922
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 7 cut(s) 4, 194, 279, 467, 878, 1019, 1310
HpyF3I CTNAG 5 cut(s) 72, 601, 760, 947, 1325
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 8 cut(s) 89, 260, 365, 476, 564, 622, 656, 1151
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 2 cut(s) 210, 1036
LweI GCATC 4 cut(s) 250, 454, 865, 923
MaeI CTAG 5 cut(s) 39, 860, 959, 1002, 1341
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 3 cut(s) 61, 115, 187
MalI GATC 6 cut(s) 420, 651, 723, 986, 1044, 1230
MboI GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
MboII GAAGA 9 cut(s) 39, 88, 126, 184, 582, 985, 1060, 1066, 1301
MfeI CAATTG 1 cut(s) 1209
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 4 cut(s) 147, 157, 509, 790
MmeI TCCRAC 2 cut(s) 615, 1261
Mox20I TGGCCA 1 cut(s) 486
MroXI GAANNNNTTC 2 cut(s) 34, 1293
MscI TGGCCA 1 cut(s) 486
MseI TTAA 5 cut(s) 203, 228, 266, 399, 903
MslI CAYNNNNRTG 3 cut(s) 90, 366, 477
Msp20I TGGCCA 1 cut(s) 486
MspR9I CCNGG 1 cut(s) 142
MunI CAATTG 1 cut(s) 1209
MvaI CCWGG 1 cut(s) 142
NcoI CCATGG 1 cut(s) 472
NdeII GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
NlaIII CATG 8 cut(s) 89, 260, 365, 476, 564, 622, 656, 1151
NlaIV GGNNCC 4 cut(s) 183, 663, 1044, 1187
NmeAIII GCCGAG 1 cut(s) 265
NspI RCATGY 3 cut(s) 365, 564, 1151
PciI ACATGT 2 cut(s) 560, 1147
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 1075
PdmI GAANNNNTTC 2 cut(s) 34, 1293
PfeI GAWTC 3 cut(s) 374, 1082, 1376
PfoI TCCNGGA 1 cut(s) 140
PkrI GCNGC 2 cut(s) 225, 1026
PleI GAGTC 4 cut(s) 147, 157, 508, 790
PpsI GAGTC 4 cut(s) 147, 157, 508, 790
Ppu21I YACGTR 1 cut(s) 685
PscI ACATGT 2 cut(s) 560, 1147
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 140
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 4 cut(s) 183, 663, 1044, 1187
PsrI GAACNNNNNNTAC 2 cut(s) 1278, 1310
PsuI RGATCY 1 cut(s) 1042
RsaI GTAC 6 cut(s) 208, 307, 452, 536, 683, 849
RsaNI GTAC 6 cut(s) 207, 306, 451, 535, 682, 848
RseI CAYNNNNRTG 3 cut(s) 90, 366, 477
SacI GAGCTC 1 cut(s) 73
SalI GTCGAC 1 cut(s) 1160
SaqAI TTAA 5 cut(s) 203, 228, 266, 399, 903
SatI GCNGC 2 cut(s) 224, 1025
Sau3AI GATC 6 cut(s) 418, 649, 721, 984, 1042, 1228
ScaI AGTACT 2 cut(s) 452, 536
SchI GAGTC 4 cut(s) 147, 157, 509, 790
ScrFI CCNGG 1 cut(s) 142
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 4 cut(s) 250, 454, 865, 923
SmiMI CAYNNNNRTG 3 cut(s) 90, 366, 477
SsiI CCGC 2 cut(s) 1301, 1355
SspI AATATT 1 cut(s) 586
SspMI CTAG 5 cut(s) 39, 860, 959, 1002, 1341
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 2 cut(s) 57, 472
TaaI ACNGT 3 cut(s) 508, 530, 922
TaiI ACGT 1 cut(s) 687
TaqI TCGA 6 cut(s) 511, 799, 931, 981, 1161, 1297
TaqII GACCGA 1 cut(s) 978
TatI WGTACW 3 cut(s) 206, 450, 534
TfiI GAWTC 3 cut(s) 374, 1082, 1376
Tru1I TTAA 5 cut(s) 203, 228, 266, 399, 903
Tru9I TTAA 5 cut(s) 203, 228, 266, 399, 903
TscAI CASTG 1 cut(s) 511
TseI GCWGC 2 cut(s) 223, 1024
TspDTI ATGAA 4 cut(s) 102, 635, 852, 1242
TspGWI ACGGA 1 cut(s) 1114
TspRI CASTG 1 cut(s) 511
XapI RAATTY 5 cut(s) 401, 625, 707, 898, 1316
XbaI TCTAGA 2 cut(s) 38, 958
XceI RCATGY 3 cut(s) 365, 564, 1151
XcmI CCANNNNNNNNNTGG 3 cut(s) 479, 1196, 1215
XmiI GTMKAC 1 cut(s) 1161
XmnI GAANNNNTTC 2 cut(s) 34, 1293
XspI CTAG 5 cut(s) 39, 860, 959, 1002, 1341
ZrmI AGTACT 2 cut(s) 452, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.