Rh4DG302900

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004206
Physical Location & Seq
Reverse (-)
52500837 .. 52506561
5725 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG302900.1

Sequence Viewer

Length: 1179 bp
ATGCAAACAAGGAAAAAAAGTTCTGGAAGAACTGCTTCTAGAGAGCATACCAGTCCCAAGGTTACAAGAGCTCAGAAGAAAACACATGAAAATGTCAAAGTATTGGATACCAAAGTTACAGATATGATTACATCTTCATCCAGAAAGCAGAGACTCGGTGGGACTCATAAGAAGAATGAGGAGCCTGTTACTGCAAGCACTTTAAGTACAAATTATGACTTGCTGCTTAATGAGACTGCCGAGGCTTGTTTGGGGCATGATGCTTTAAGTGATGATTGCAAAGAGAATAAGGGTTGTGAGGAGGGTGCTGCTGATTGCGTTCTGGGAACTATATTTTCTCCTGCCTTTCACATACCGAAACATGCTGGTGGAGAATCTGCCAATGGAGTTGATTTTGTTAAATTCTTTCAAAATGGAGATCACAATTTCCATAAAGAGATGGAAATAAAGTACTCACCCGATGATGCACACCATGGTTACGTTGGCCAAGAGACATCTGAGTCCACTGTTCGAGACGAGAACAACTACTTTGTGGAGGCAACCAATATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCCAAGTTGGAATGTTTAGATGAGCATGGTCAGGAACCTATGTCAACTGATTTGTACGTGGATGATGATGAGAGCGAGGAATTTGATGACTTTGATCCTTACTTATTTATCAAGAACTTACCTGACTTGTCCTCAGTTGTCCCCACTTTTCGGCCGTTGCTGCTGCCTAAGCAGACTCGAAGTTGTCCTCCTACAAGTCTTGTTTTGGACTTGGATGAAACTCTGGTACATTCCACACTAGAACCTTGTGATGATGCAGACTTCACATTTCCTGTAAATTTTAACCTACAAGAGCATACAGTATATGTTCGATGCCGTCCTCATCTCAGAGATTTTCTGGACAGAGTTTCGCTTCTTTTCGAGATCATTATCTTCACAGCTAGTCAAAGTATCTATGCAGAGCAGCTTCTAAATGTGCTGGATCCGAAGAGGAAGATTTTTCGCCATCGTGTTTTTCGTGATTCCTGTGTTTTTGTGGAGGGGAATTATCTCAAAGATTTGTCGGTTCTTGGCCGTGATTTGGCACGTGTTGTCATAGTCGACAACTCTCCACAGGTATACTATTCCCACCTCCCTCCATCCTTCAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

392

Amino Acids

44.35

Weight (kDa)

5.27

Isoelectric Point (pI)

43.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 263 - 386 7.3e-44 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
AccI GTMKAC 2 cut(s) 1128, 1146
AclWI GGATC 3 cut(s) 677, 1004, 1017
AcoI YGGCCR 3 cut(s) 484, 740, 1099
AcsI RAATTY 4 cut(s) 401, 586, 668, 865
AcuI CTGAAG 2 cut(s) 576, 1156
AcvI CACGTG 1 cut(s) 1115
AfaI GTAC 4 cut(s) 208, 452, 644, 816
AfiI CCNNNNNNNGG 2 cut(s) 738, 1108
AflIII ACRYGT 1 cut(s) 1114
AgsI TTSAA 1 cut(s) 410
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 3 cut(s) 71, 968, 994
AluI AGCT 3 cut(s) 71, 968, 994
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 5 cut(s) 145, 227, 485, 507, 565
AlwI GGATC 3 cut(s) 677, 1004, 1017
AoxI GGCC 3 cut(s) 484, 740, 1099
ApeKI GCWGC 5 cut(s) 223, 308, 748, 751, 991
ApoI RAATTY 4 cut(s) 401, 586, 668, 865
Asp700I GAANNNNTTC 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 447
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BalI TGGCCA 1 cut(s) 486
BamHI GGATCC 1 cut(s) 1009
BanII GRGCYC 1 cut(s) 73
BbrPI CACGTG 1 cut(s) 1115
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 5 cut(s) 210, 295, 735, 738, 1003
BccI CCATC 3 cut(s) 433, 1041, 1174
BceAI ACGGC 3 cut(s) 727, 888, 1086
BciVI GTATCC 1 cut(s) 100
BcoDI GTCTC 5 cut(s) 145, 227, 485, 507, 565
BfaI CTAG 3 cut(s) 39, 827, 969
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 5 cut(s) 224, 309, 749, 752, 992
BlsI GCNGC 5 cut(s) 225, 310, 750, 753, 993
BmcAI AGTACT 1 cut(s) 452
BmiI GGNNCC 3 cut(s) 183, 624, 1011
BmsI GCATC 4 cut(s) 250, 454, 832, 890
Bpu10I CCTNAGC 1 cut(s) 756
BsaAI YACGTR 2 cut(s) 646, 1115
BsaBI GATNNNNATC 1 cut(s) 956
BsaJI CCNNGG 3 cut(s) 57, 240, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 738, 1108
Bse1I ACTGG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 956
BseDI CCNNGG 3 cut(s) 57, 240, 472
BseGI GGATG 4 cut(s) 137, 655, 808, 1166
BseJI GATNNNNATC 1 cut(s) 956
BseLI CCNNNNNNNGG 2 cut(s) 738, 1108
BseMII CTCAG 5 cut(s) 86, 489, 576, 735, 928
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 2 cut(s) 194, 314
BseX3I CGGCCG 1 cut(s) 740
BseXI GCAGC 5 cut(s) 210, 295, 735, 738, 1003
Bsh1285I CGRYCG 1 cut(s) 743
BshFI GGCC 3 cut(s) 486, 742, 1101
BsiEI CGRYCG 1 cut(s) 743
BsiHKAI GWGCWC 1 cut(s) 73
BslFI GGGAC 3 cut(s) 39, 175, 713
BslI CCNNNNNNNGG 2 cut(s) 738, 1108
BsmAI GTCTC 5 cut(s) 145, 227, 485, 507, 565
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 3 cut(s) 39, 175, 713
BsnI GGCC 3 cut(s) 486, 742, 1101
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 4 cut(s) 418, 682, 951, 1009
Bsp19I CCATGG 1 cut(s) 472
BspANI GGCC 3 cut(s) 486, 742, 1101
BspCNI CTCAG 5 cut(s) 85, 490, 575, 734, 927
BspLI GGNNCC 3 cut(s) 183, 624, 1011
BspPI GGATC 3 cut(s) 677, 1004, 1017
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 57, 240, 472
BssMI GATC 4 cut(s) 418, 682, 951, 1009
BssNAI GTATAC 1 cut(s) 1147
BssT1I CCWWGG 2 cut(s) 57, 472
Bst1107I GTATAC 1 cut(s) 1147
Bst4CI ACNGT 2 cut(s) 508, 889
Bst6I CTCTTC 1 cut(s) 1010
BstBAI YACGTR 2 cut(s) 646, 1115
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 6 cut(s) 72, 498, 562, 721, 756, 914
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 4 cut(s) 137, 655, 808, 1166
BstKTI GATC 4 cut(s) 421, 685, 954, 1012
BstMAI GTCTC 5 cut(s) 145, 227, 485, 507, 565
BstMBI GATC 4 cut(s) 418, 682, 951, 1009
BstMCI CGRYCG 1 cut(s) 743
BstMWI GCNNNNNNNGC 2 cut(s) 748, 757
BstNSI RCATGY 1 cut(s) 365
BstV1I GCAGC 5 cut(s) 210, 295, 735, 738, 1003
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BstZ17I GTATAC 1 cut(s) 1147
BstZI CGGCCG 1 cut(s) 740
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 3 cut(s) 486, 742, 1101
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 4 cut(s) 137, 655, 808, 1166
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 196
Csp6I GTAC 4 cut(s) 207, 451, 643, 815
CviAII CATG 6 cut(s) 86, 257, 362, 473, 580, 614
CviJI RGCY 9 cut(s) 71, 184, 245, 486, 566, 742, 968, 994, 1101
CviKI_1 RGCY 9 cut(s) 71, 184, 245, 486, 566, 742, 968, 994, 1101
CviQI GTAC 4 cut(s) 207, 451, 643, 815
DdeI CTNAG 6 cut(s) 72, 498, 562, 721, 756, 914
DpnI GATC 4 cut(s) 420, 684, 953, 1011
DpnII GATC 4 cut(s) 418, 682, 951, 1009
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 3 cut(s) 484, 740, 1099
EagI CGGCCG 1 cut(s) 740
Eam1104I CTCTTC 1 cut(s) 1010
EarI CTCTTC 1 cut(s) 1010
Ecl136II GAGCTC 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 740
Eco130I CCWWGG 2 cut(s) 57, 472
Eco24I GRGCYC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 740
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 576, 1156
Eco72I CACGTG 1 cut(s) 1115
EcoICRI GAGCTC 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 57, 472
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 57, 472
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 6 cut(s) 89, 260, 365, 476, 583, 617
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 3 cut(s) 39, 175, 713
FatI CATG 6 cut(s) 85, 256, 361, 472, 579, 613
FblI GTMKAC 2 cut(s) 1128, 1146
Fnu4HI GCNGC 5 cut(s) 224, 309, 749, 752, 992
FokI GGATG 4 cut(s) 124, 662, 815, 1153
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 5 cut(s) 224, 309, 749, 752, 992
FspBI CTAG 3 cut(s) 39, 827, 969
GluI GCNGC 5 cut(s) 224, 309, 749, 752, 992
HaeIII GGCC 3 cut(s) 486, 742, 1101
Hin1II CATG 6 cut(s) 89, 260, 365, 476, 583, 617
HincII GTYRAC 2 cut(s) 633, 1129
HindII GTYRAC 2 cut(s) 633, 1129
HinfI GANTC 6 cut(s) 153, 163, 374, 500, 763, 1049
HphI GGTGA 1 cut(s) 447
Hpy166II GTNNAC 4 cut(s) 504, 633, 1129, 1147
Hpy188I TCNGA 6 cut(s) 75, 499, 556, 917, 1014, 1175
Hpy188III TCNNGA 9 cut(s) 24, 39, 141, 512, 620, 700, 926, 949, 1046
Hpy8I GTNNAC 4 cut(s) 504, 633, 1129, 1147
HpyCH4III ACNGT 2 cut(s) 508, 889
HpyCH4IV ACGT 3 cut(s) 480, 645, 1114
HpyCH4V TGCA 6 cut(s) 4, 194, 279, 467, 845, 986
HpyF10VI GCNNNNNNNGC 2 cut(s) 748, 757
HpyF3I CTNAG 6 cut(s) 72, 498, 562, 721, 756, 914
HpySE526I ACGT 3 cut(s) 480, 645, 1114
Hsp92II CATG 6 cut(s) 89, 260, 365, 476, 583, 617
Kzo9I GATC 4 cut(s) 418, 682, 951, 1009
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 5 cut(s) 210, 295, 735, 738, 1003
LweI GCATC 4 cut(s) 250, 454, 832, 890
MaeI CTAG 3 cut(s) 39, 827, 969
MaeII ACGT 3 cut(s) 480, 645, 1114
MaeIII GTNAC 4 cut(s) 61, 115, 187, 476
MalI GATC 4 cut(s) 420, 684, 953, 1011
MboI GATC 4 cut(s) 418, 682, 951, 1009
MboII GAAGA 8 cut(s) 39, 88, 126, 184, 543, 952, 1027, 1033
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 486
MluCI AATT 7 cut(s) 211, 401, 424, 586, 668, 865, 1072
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 4 cut(s) 147, 157, 509, 757
MmeI TCCRAC 1 cut(s) 576
Mox20I TGGCCA 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 34
MscI TGGCCA 1 cut(s) 486
MseI TTAA 5 cut(s) 203, 228, 266, 399, 870
MslI CAYNNNNRTG 2 cut(s) 90, 366
Msp20I TGGCCA 1 cut(s) 486
MwoI GCNNNNNNNGC 2 cut(s) 748, 757
NcoI CCATGG 1 cut(s) 472
NdeII GATC 4 cut(s) 418, 682, 951, 1009
NlaIII CATG 6 cut(s) 89, 260, 365, 476, 583, 617
NlaIV GGNNCC 3 cut(s) 183, 624, 1011
NmeAIII GCCGAG 1 cut(s) 265
NspI RCATGY 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 1042
PdmI GAANNNNTTC 1 cut(s) 34
PfeI GAWTC 2 cut(s) 374, 1049
PkrI GCNGC 5 cut(s) 225, 310, 750, 753, 993
PleI GAGTC 4 cut(s) 147, 157, 508, 757
PmaCI CACGTG 1 cut(s) 1115
PmlI CACGTG 1 cut(s) 1115
PpsI GAGTC 4 cut(s) 147, 157, 508, 757
Ppu21I YACGTR 2 cut(s) 646, 1115
Psp124BI GAGCTC 1 cut(s) 73
PspCI CACGTG 1 cut(s) 1115
PspN4I GGNNCC 3 cut(s) 183, 624, 1011
PsuI RGATCY 1 cut(s) 1009
RsaI GTAC 4 cut(s) 208, 452, 644, 816
RsaNI GTAC 4 cut(s) 207, 451, 643, 815
RseI CAYNNNNRTG 2 cut(s) 90, 366
SacI GAGCTC 1 cut(s) 73
SalI GTCGAC 1 cut(s) 1127
SaqAI TTAA 5 cut(s) 203, 228, 266, 399, 870
SatI GCNGC 5 cut(s) 224, 309, 749, 752, 992
Sau3AI GATC 4 cut(s) 418, 682, 951, 1009
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 4 cut(s) 147, 157, 509, 757
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 4 cut(s) 250, 454, 832, 890
SmiMI CAYNNNNRTG 2 cut(s) 90, 366
Sse9I AATT 7 cut(s) 211, 401, 424, 586, 668, 865, 1072
SspI AATATT 1 cut(s) 547
SspMI CTAG 3 cut(s) 39, 827, 969
SstI GAGCTC 1 cut(s) 73
StyI CCWWGG 2 cut(s) 57, 472
TaaI ACNGT 2 cut(s) 508, 889
TaiI ACGT 3 cut(s) 483, 648, 1117
TaqI TCGA 5 cut(s) 511, 766, 898, 948, 1128
TasI AATT 7 cut(s) 211, 401, 424, 586, 668, 865, 1072
TatI WGTACW 2 cut(s) 206, 450
TfiI GAWTC 2 cut(s) 374, 1049
Tru1I TTAA 5 cut(s) 203, 228, 266, 399, 870
Tru9I TTAA 5 cut(s) 203, 228, 266, 399, 870
TscAI CASTG 1 cut(s) 511
TseI GCWGC 5 cut(s) 223, 308, 748, 751, 991
TspDTI ATGAA 4 cut(s) 102, 126, 596, 819
TspRI CASTG 1 cut(s) 511
XapI RAATTY 4 cut(s) 401, 586, 668, 865
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 479
XmiI GTMKAC 2 cut(s) 1128, 1146
XmnI GAANNNNTTC 1 cut(s) 34
XspI CTAG 3 cut(s) 39, 827, 969
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.