MD13G1118600.v1.1

CTD small phosphatase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004206
Physical Location & Seq
Forward (+)
8672897 .. 8677369
4473 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1118600.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGCAAACGAAGAAAAGAAGTTCCGGAAGAACTGCTGCGAGAGAGCATGCTTGTCCCAAGGTTACAAGAGCTCAGAAGAAAGCATATGAAAGTGTGCAAGTTATGGAAAAGAAAGTTGCTGACCTAATTACATCTTCAGCAAGGAAGCAGAGAATAGGTGGCACTCATAAAAAGAATGGGGGGCCTTTTACTGCAACCAATTCAGATTTGAATTATGACTCAATGCGTGATGGGACTGCTGACACTTGTTTGGGGCATGATGCTATGCGTGATGATTGCATAGATATTAAGGATTGTAAAGAGGGAACTACTGATTGCAAAGCGGAAACTATATTTTCTCCTGCCTTTCACATATCTAAACATGCCGAAGGGGAATCTGATAATGGAGTTGTGGATCCAGCCACTATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCCAAGTTGGAATGTGTGGATGAACATGGTCACGAGCCCATGTCAACTGATTTATACACAGATGATGACGAGTATGAGGAAGTTGATGACTTTGATCCTTACTTTTTTATCAAGAACTTACCAGACTTATCAGCAGTTGTTCCAACTTTTCGGCCGATGCTTTTGCCTAAACAGACACGGCGTTGTCCTCCTACTAGTCTTGTTTTGGATTTGGATGAAACTTTGGTACACTCCACACTAGAACCTTGTGCTGATGCAGACTTCACTTTTGCTGTAAATTTTAACCTCCAAAAGCATACAGTATATGTCCGATGCCGTCCTCATCTCAGAGATTTTCTGGACAAAGTTTCCAATCTTTTTGAGATCATAATATTTACAGCTAGTCAAAGTATTTATGCAGAGCAACTTCTAAATGTGCTGGACCCAAAAAGGAAGATATTTCGCCATCGTGTTTTTCGGGATTCCTGTGTTTTTGTGGAGGGGAATTACCTCAAAGATTTGTCAGTTCTTGGTCGTGATTTGGCACACACTATCATAATCGACAACTCTCCACAGGCATTCGGGTTCCAAGTAGACAATGGAATTCCAATTGAGAGCTGGTTTGATGATCGTTCTGATAAAGAATTACTCTTCTTACTTCCTTTTTTGGAGAGCTTGGTTGGAGTAGAAGATGTTCGGCCACTAATTGCAAGCAAATTCAGCCTTAGGGAGAAAATAGCTGCAGCTGCTTATCCTCTTAACTCAAACAGAGGCGATCCTTTCGAAAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

406

Amino Acids

45.63

Weight (kDa)

5.38

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 216 - 375 6.5e-59 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1020
AccIII TCCGGA 1 cut(s) 23
AciI CCGC 1 cut(s) 323
AclWI GGATC 4 cut(s) 389, 402, 536, 1196
AcoI YGGCCR 2 cut(s) 599, 1124
AcsI RAATTY 4 cut(s) 445, 724, 1029, 1142
AcuI CTGAAG 2 cut(s) 120, 435
AfaI GTAC 1 cut(s) 675
AgsI TTSAA 1 cut(s) 211
AhlI ACTAGT 1 cut(s) 641
AluBI AGCT 6 cut(s) 71, 827, 1044, 1101, 1166, 1172
AluI AGCT 6 cut(s) 71, 827, 1044, 1101, 1166, 1172
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 424
AlwI GGATC 4 cut(s) 389, 402, 536, 1196
Aor13HI TCCGGA 1 cut(s) 23
AoxI GGCC 3 cut(s) 182, 599, 1124
ApeKI GCWGC 4 cut(s) 35, 1166, 1169, 1172
ApoI RAATTY 4 cut(s) 445, 724, 1029, 1142
ArsI GACNNNNNNTTYG 2 cut(s) 698, 730
Asp700I GAANNNNTTC 1 cut(s) 1119
AspS9I GGNCC 2 cut(s) 182, 868
AsuII TTCGAA 1 cut(s) 1209
AvaII GGWCC 1 cut(s) 868
AxyI CCTNAGG 1 cut(s) 1151
BamHI GGATCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 73, 486
BauI CACGAG 1 cut(s) 479
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 22, 1153, 1159, 1181
BccI CCATC 2 cut(s) 224, 900
BceAI ACGGC 2 cut(s) 641, 747
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BcoDI GTCTC 1 cut(s) 424
BcuI ACTAGT 1 cut(s) 641
BfaI CTAG 3 cut(s) 642, 686, 828
BfmI CTRYAG 1 cut(s) 1167
BisI GCNGC 4 cut(s) 36, 1167, 1170, 1173
BlsI GCNGC 4 cut(s) 37, 1168, 1171, 1174
Bme18I GGWCC 1 cut(s) 868
BmgT120I GGNCC 2 cut(s) 182, 868
BmiI GGNNCC 4 cut(s) 183, 396, 870, 1013
BmsI GCATC 4 cut(s) 250, 594, 691, 749
Bpu14I TTCGAA 1 cut(s) 1209
BsaJI CCNNGG 1 cut(s) 57
BsaWI WCCGGW 1 cut(s) 23
Bse21I CCTNAGG 1 cut(s) 1151
BseAI TCCGGA 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 472, 667
BseMII CTCAG 3 cut(s) 86, 435, 787
BseX3I CGGCCG 1 cut(s) 599
BseXI GCAGC 4 cut(s) 22, 1153, 1159, 1181
Bsh1285I CGRYCG 1 cut(s) 602
BshFI GGCC 3 cut(s) 184, 601, 1126
BsiEI CGRYCG 1 cut(s) 602
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 24
BslFI GGGAC 2 cut(s) 39, 247
BsmAI GTCTC 1 cut(s) 424
BsmFI GGGAC 2 cut(s) 39, 247
BsmI GAATGC 1 cut(s) 1004
BsnI GGCC 3 cut(s) 184, 601, 1126
Bsp119I TTCGAA 1 cut(s) 1209
Bsp1286I GDGCHC 2 cut(s) 73, 486
Bsp13I TCCGGA 1 cut(s) 23
Bsp143I GATC 5 cut(s) 394, 541, 810, 1054, 1201
BspACI CCGC 1 cut(s) 323
BspANI GGCC 3 cut(s) 184, 601, 1126
BspCNI CTCAG 3 cut(s) 85, 434, 786
BspEI TCCGGA 1 cut(s) 23
BspLI GGNNCC 4 cut(s) 183, 396, 870, 1013
BspMAI CTGCAG 1 cut(s) 1171
BspPI GGATC 4 cut(s) 389, 402, 536, 1196
BspT104I TTCGAA 1 cut(s) 1209
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 5 cut(s) 394, 541, 810, 1054, 1201
BssSI CACGAG 1 cut(s) 479
BssT1I CCWWGG 1 cut(s) 57
Bst2BI CACGAG 1 cut(s) 479
Bst4CI ACNGT 1 cut(s) 748
Bst6I CTCTTC 1 cut(s) 1082
BstBI TTCGAA 1 cut(s) 1209
BstC8I GCNNGC 2 cut(s) 48, 1138
BstDEI CTNAG 4 cut(s) 72, 421, 773, 1151
BstF5I GGATG 2 cut(s) 472, 667
BstKTI GATC 5 cut(s) 397, 544, 813, 1057, 1204
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 5 cut(s) 394, 541, 810, 1054, 1201
BstMCI CGRYCG 1 cut(s) 602
BstMWI GCNNNNNNNGC 2 cut(s) 1146, 1172
BstNSI RCATGY 2 cut(s) 50, 365
BstSFI CTRYAG 1 cut(s) 1167
BstV1I GCAGC 4 cut(s) 22, 1153, 1159, 1181
BstX2I RGATCY 1 cut(s) 394
BstYI RGATCY 1 cut(s) 394
BstZI CGGCCG 1 cut(s) 599
Bsu36I CCTNAGG 1 cut(s) 1151
BsuRI GGCC 3 cut(s) 184, 601, 1126
BtsCI GGATG 2 cut(s) 472, 667
Cac8I GCNNGC 2 cut(s) 48, 1138
Cfr13I GGNCC 2 cut(s) 182, 868
Csp6I GTAC 1 cut(s) 674
CviAII CATG 6 cut(s) 47, 257, 362, 439, 473, 487
CviQI GTAC 1 cut(s) 674
DdeI CTNAG 4 cut(s) 72, 421, 773, 1151
DpnI GATC 5 cut(s) 396, 543, 812, 1056, 1203
DpnII GATC 5 cut(s) 394, 541, 810, 1054, 1201
EaeI YGGCCR 2 cut(s) 599, 1124
EagI CGGCCG 1 cut(s) 599
Eam1104I CTCTTC 1 cut(s) 1082
EarI CTCTTC 1 cut(s) 1082
Ecl136II GAGCTC 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 599
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 2 cut(s) 73, 486
Eco47I GGWCC 1 cut(s) 868
Eco52I CGGCCG 1 cut(s) 599
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 2 cut(s) 120, 435
Eco81I CCTNAGG 1 cut(s) 1151
EcoICRI GAGCTC 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 182
EcoRI GAATTC 1 cut(s) 1029
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 2 cut(s) 73, 486
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 6 cut(s) 50, 260, 365, 442, 476, 490
FaqI GGGAC 2 cut(s) 39, 247
FatI CATG 6 cut(s) 46, 256, 361, 438, 472, 486
FauNDI CATATG 1 cut(s) 85
FblI GTMKAC 1 cut(s) 1020
Fnu4HI GCNGC 4 cut(s) 36, 1167, 1170, 1173
FokI GGATG 2 cut(s) 479, 674
FriOI GRGCYC 2 cut(s) 73, 486
Fsp4HI GCNGC 4 cut(s) 36, 1167, 1170, 1173
FspBI CTAG 3 cut(s) 642, 686, 828
GluI GCNGC 4 cut(s) 36, 1167, 1170, 1173
HaeIII GGCC 3 cut(s) 184, 601, 1126
HapII CCGG 1 cut(s) 24
Hin1II CATG 6 cut(s) 50, 260, 365, 442, 476, 490
HincII GTYRAC 1 cut(s) 492
HindII GTYRAC 1 cut(s) 492
HinfI GANTC 3 cut(s) 218, 374, 908
HpaII CCGG 1 cut(s) 24
Hpy166II GTNNAC 3 cut(s) 492, 676, 1021
Hpy188I TCNGA 7 cut(s) 75, 205, 379, 415, 758, 776, 1063
Hpy188III TCNNGA 6 cut(s) 24, 479, 559, 785, 905, 962
Hpy8I GTNNAC 3 cut(s) 492, 676, 1021
HpyAV CCTTC 1 cut(s) 362
HpyCH4III ACNGT 1 cut(s) 748
HpyCH4V TGCA 9 cut(s) 4, 97, 194, 279, 318, 704, 845, 1136, 1169
HpyF10VI GCNNNNNNNGC 2 cut(s) 1146, 1172
HpyF3I CTNAG 4 cut(s) 72, 421, 773, 1151
Hsp92II CATG 6 cut(s) 50, 260, 365, 442, 476, 490
Kpn2I TCCGGA 1 cut(s) 23
Kzo9I GATC 5 cut(s) 394, 541, 810, 1054, 1201
LpnPI CCDG 9 cut(s) 37, 354, 411, 582, 770, 851, 925, 986, 1030
Lsp1109I GCAGC 4 cut(s) 22, 1153, 1159, 1181
LweI GCATC 4 cut(s) 250, 594, 691, 749
MaeI CTAG 3 cut(s) 642, 686, 828
MaeIII GTNAC 2 cut(s) 61, 476
MalI GATC 5 cut(s) 396, 543, 812, 1056, 1203
MboI GATC 5 cut(s) 394, 541, 810, 1054, 1201
MboII GAAGA 8 cut(s) 22, 39, 88, 126, 402, 892, 1069, 1127
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 1 cut(s) 394
MhlI GDGCHC 2 cut(s) 73, 486
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 3 cut(s) 435, 614, 1087
MnlI CCTC 9 cut(s) 295, 517, 645, 743, 777, 919, 947, 1190, 1191
MroI TCCGGA 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 3 cut(s) 288, 729, 1185
MspA1I CMGCKG 1 cut(s) 1172
MspI CCGG 1 cut(s) 24
MunI CAATTG 1 cut(s) 1035
Mva1269I GAATGC 1 cut(s) 1004
MwoI GCNNNNNNNGC 2 cut(s) 1146, 1172
NdeI CATATG 1 cut(s) 85
NdeII GATC 5 cut(s) 394, 541, 810, 1054, 1201
NlaIII CATG 6 cut(s) 50, 260, 365, 442, 476, 490
NlaIV GGNNCC 4 cut(s) 183, 396, 870, 1013
NmuCI GTSAC 1 cut(s) 476
NspI RCATGY 2 cut(s) 50, 365
NspV TTCGAA 1 cut(s) 1209
PaeI GCATGC 1 cut(s) 50
PctI GAATGC 1 cut(s) 1004
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 2 cut(s) 374, 908
PkrI GCNGC 4 cut(s) 37, 1168, 1171, 1174
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 73
PspN4I GGNNCC 4 cut(s) 183, 396, 870, 1013
PspPI GGNCC 2 cut(s) 182, 868
PsrI GAACNNNNNNTAC 2 cut(s) 1104, 1136
PstI CTGCAG 1 cut(s) 1171
PsuI RGATCY 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 1172
RsaI GTAC 1 cut(s) 675
RsaNI GTAC 1 cut(s) 674
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 3 cut(s) 288, 729, 1185
SatI GCNGC 4 cut(s) 36, 1167, 1170, 1173
Sau3AI GATC 5 cut(s) 394, 541, 810, 1054, 1201
Sau96I GGNCC 2 cut(s) 182, 868
SchI GAGTC 1 cut(s) 212
SduI GDGCHC 2 cut(s) 73, 486
SfaNI GCATC 4 cut(s) 250, 594, 691, 749
SfcI CTRYAG 1 cut(s) 1167
SfuI TTCGAA 1 cut(s) 1209
SinI GGWCC 1 cut(s) 868
SpeI ACTAGT 1 cut(s) 641
SphI GCATGC 1 cut(s) 50
SsiI CCGC 1 cut(s) 323
SspI AATATT 1 cut(s) 819
SspMI CTAG 3 cut(s) 642, 686, 828
SstI GAGCTC 1 cut(s) 73
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 1 cut(s) 748
TaqI TCGA 2 cut(s) 987, 1209
TfiI GAWTC 2 cut(s) 374, 908
Tru1I TTAA 3 cut(s) 288, 729, 1185
Tru9I TTAA 3 cut(s) 288, 729, 1185
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 4 cut(s) 35, 1166, 1169, 1172
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 4 cut(s) 102, 455, 483, 678
VpaK11BI GGWCC 1 cut(s) 868
XapI RAATTY 4 cut(s) 445, 724, 1029, 1142
XceI RCATGY 2 cut(s) 50, 365
XcmI CCANNNNNNNNNTGG 2 cut(s) 1022, 1041
XmiI GTMKAC 1 cut(s) 1020
XmnI GAANNNNTTC 1 cut(s) 1119
XspI CTAG 3 cut(s) 642, 686, 828
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.