Rw4G025950

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004206
Physical Location & Seq
Reverse (-)
52083139 .. 52088217
5079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G025950.1

Sequence Viewer

Length: 1401 bp
ATGCAAACAAGGAAAAAAAGTTCTGGAAGAACTGCTTCTAGAGAGCATACCAGTCCCAAGGTTACAAGAGCTCAGAAGAAAACACATGAAAATGTCAAAGTATTGGATACCAAAGTTACAGATATGATTACATCTTCATCCAGAAAGCAGAGACTCGGTGGGACTCATAAGAAGAATGAGGAGCCTGTTACTGCAAGCACTTTAAGTACAAATTATGACTTGCTGCTTAATGAGACTGCCGAGGCTTGTTTGGGGCATGATGCTTTAAGTGATGATTGCAAAGAGAATAAGGGTTGTGAGGAGGGTGCTGCTGATTGCGTTCTGGGAACTATATTTTCTCCTGCCTTTCACATACCGAAACATGCTGGTGGAGAATCTGCCAATGGAGTTGATTTTGTTAAATTCTTTCAAAATGGAGATCACAATTTCCATAAAGAGATGGAAATAAAGTACTCACCCGATGATGCACACCATGGTTATGTTGGCCAAGAGACATCTGAGTCCACTGTTCGAGACGAGAACAACTACTGTGAGAACTCATTATCTTCTGTAACTATAACATGTCCTGTTGTGGAGGCAACCAATATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCCAAGTTGGAATGTTTAGATGAGCATGGTCAGGAACCTATGTCAACTGATTTGTACGTGGATGATGATGAGAGCGAGGAATTTGATGACTTTGATCCTTACTTATTTATCAAGAACTTACCTGACTTGTCCTCAGTTGTCCCCACTTTTCGGCCGTTGCTGCTGCCTAAGCAGACTCGAAGTTGTCCTCCTACAAGTCTTGTTTTGGACTTGGATGAAACTCTGGTACATTCCACACTAGAACCTTGTGATGATGCAGACTTCACATTTCCTGTAAATTTTAACCTACAAGAGCATACAGTATATGTTCGATGCCGTCCTCATCTCAGAGATTTTCTGGACAGAGTTTCGCTTCTTTTCGAGATCATTATCTTCACAGCTAGTCAAAGTATCTATGCCGAGCAGCTTCTAAATGTGCTGGATCCGAAGAGGAAGATTTTTCGCCATCGTGTTTTTCGTGATTCCTGTGTTTTTGTGGAGGGGAATTATCTCAAAGATTTGTCGGTTCTTGGCCGTGATTTGGCACGTGTTGTCATAGTCGACAACTCTCCACAGGCATTTGGGTTCCAAGTGGACAATGGAATACCGATTGAGAGCTGGTTTGATGATCGTTCTGATACAGAATTACTTTCGTTACTTCCTTTTTTGGAAAGCTTGGTTGGAGTAGAAGATGTTCGACCGCTAATTGCAAACAAATTCAGCCTCCGGGAGAAAATAGCTACTGCTGATTATCCACTAAACTCAAATAGAGGTGATTCTTTAGTGCCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

466

Amino Acids

52.31

Weight (kDa)

4.94

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 276 - 435 1e-59 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
AccI GTMKAC 1 cut(s) 1167
AciI CCGC 1 cut(s) 1307
AclWI GGATC 3 cut(s) 716, 1043, 1056
AcoI YGGCCR 3 cut(s) 484, 779, 1138
AcsI RAATTY 5 cut(s) 401, 625, 707, 904, 1322
AcuI CTGAAG 1 cut(s) 615
AcvI CACGTG 1 cut(s) 1154
AfaI GTAC 4 cut(s) 208, 452, 683, 855
AfiI CCNNNNNNNGG 2 cut(s) 777, 1147
AflIII ACRYGT 2 cut(s) 560, 1153
AgsI TTSAA 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 1269, 1301
AloI GAACNNNNNNTCC 1 cut(s) 36
AluBI AGCT 6 cut(s) 71, 1007, 1033, 1224, 1281, 1346
AluI AGCT 6 cut(s) 71, 1007, 1033, 1224, 1281, 1346
Alw21I GWGCWC 1 cut(s) 73
Alw26I GTCTC 5 cut(s) 145, 227, 485, 507, 604
AlwI GGATC 3 cut(s) 716, 1043, 1056
AoxI GGCC 3 cut(s) 484, 779, 1138
ApeKI GCWGC 5 cut(s) 223, 308, 787, 790, 1030
ApoI RAATTY 5 cut(s) 401, 625, 707, 904, 1322
Asp700I GAANNNNTTC 2 cut(s) 34, 1299
AsuC2I CCSGG 1 cut(s) 1334
AsuHPI GGTGA 2 cut(s) 447, 1391
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BalI TGGCCA 1 cut(s) 486
BamHI GGATCC 1 cut(s) 1048
BanII GRGCYC 1 cut(s) 73
BbrPI CACGTG 1 cut(s) 1154
Bbv12I GWGCWC 1 cut(s) 73
BbvI GCAGC 5 cut(s) 210, 295, 774, 777, 1042
BccI CCATC 2 cut(s) 433, 1080
BceAI ACGGC 3 cut(s) 766, 927, 1125
BciVI GTATCC 1 cut(s) 100
BcnI CCSGG 1 cut(s) 1334
BcoDI GTCTC 5 cut(s) 145, 227, 485, 507, 604
BfaI CTAG 3 cut(s) 39, 866, 1008
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 5 cut(s) 224, 309, 788, 791, 1031
BlsI GCNGC 5 cut(s) 225, 310, 789, 792, 1032
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 1 cut(s) 1334
BmiI GGNNCC 4 cut(s) 183, 663, 1050, 1193
BmrFI CCNGG 1 cut(s) 1334
BmsI GCATC 4 cut(s) 250, 454, 871, 929
Bpu10I CCTNAGC 1 cut(s) 795
BpuMI CCSGG 1 cut(s) 1334
BsaAI YACGTR 2 cut(s) 685, 1154
BsaBI GATNNNNATC 1 cut(s) 995
BsaJI CCNNGG 3 cut(s) 57, 240, 472
Bsc4I CCNNNNNNNGG 2 cut(s) 777, 1147
Bse1I ACTGG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 995
BseDI CCNNGG 3 cut(s) 57, 240, 472
BseGI GGATG 3 cut(s) 137, 694, 847
BseJI GATNNNNATC 1 cut(s) 995
BseLI CCNNNNNNNGG 2 cut(s) 777, 1147
BseMII CTCAG 5 cut(s) 86, 489, 615, 774, 967
BseNI ACTGG 1 cut(s) 51
BseRI GAGGAG 2 cut(s) 194, 314
BseX3I CGGCCG 1 cut(s) 779
BseXI GCAGC 5 cut(s) 210, 295, 774, 777, 1042
Bsh1285I CGRYCG 2 cut(s) 782, 1307
BshFI GGCC 3 cut(s) 486, 781, 1140
BsiEI CGRYCG 2 cut(s) 782, 1307
BsiHKAI GWGCWC 1 cut(s) 73
BsiSI CCGG 1 cut(s) 1333
BslFI GGGAC 3 cut(s) 39, 175, 752
BslI CCNNNNNNNGG 2 cut(s) 777, 1147
BsmAI GTCTC 5 cut(s) 145, 227, 485, 507, 604
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 3 cut(s) 39, 175, 752
BsnI GGCC 3 cut(s) 486, 781, 1140
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 5 cut(s) 418, 721, 990, 1048, 1234
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 1 cut(s) 1307
BspANI GGCC 3 cut(s) 486, 781, 1140
BspCNI CTCAG 5 cut(s) 85, 490, 614, 773, 966
BspLI GGNNCC 4 cut(s) 183, 663, 1050, 1193
BspPI GGATC 3 cut(s) 716, 1043, 1056
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 3 cut(s) 57, 240, 472
BssMI GATC 5 cut(s) 418, 721, 990, 1048, 1234
BssT1I CCWWGG 2 cut(s) 57, 472
Bst4CI ACNGT 3 cut(s) 508, 530, 928
Bst6I CTCTTC 1 cut(s) 1049
BstBAI YACGTR 2 cut(s) 685, 1154
BstC8I GCNNGC 1 cut(s) 196
BstDEI CTNAG 6 cut(s) 72, 498, 601, 760, 795, 953
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 3 cut(s) 137, 694, 847
BstKTI GATC 5 cut(s) 421, 724, 993, 1051, 1237
BstMAI GTCTC 5 cut(s) 145, 227, 485, 507, 604
BstMBI GATC 5 cut(s) 418, 721, 990, 1048, 1234
BstMCI CGRYCG 2 cut(s) 782, 1307
BstMWI GCNNNNNNNGC 2 cut(s) 787, 796
BstNSI RCATGY 2 cut(s) 365, 564
BstSCI CCNGG 1 cut(s) 1332
BstV1I GCAGC 5 cut(s) 210, 295, 774, 777, 1042
BstX2I RGATCY 1 cut(s) 1048
BstYI RGATCY 1 cut(s) 1048
BstZI CGGCCG 1 cut(s) 779
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 3 cut(s) 486, 781, 1140
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 3 cut(s) 137, 694, 847
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 196
Csp6I GTAC 4 cut(s) 207, 451, 682, 854
CviAII CATG 7 cut(s) 86, 257, 362, 473, 561, 619, 653
CviQI GTAC 4 cut(s) 207, 451, 682, 854
DdeI CTNAG 6 cut(s) 72, 498, 601, 760, 795, 953
DpnI GATC 5 cut(s) 420, 723, 992, 1050, 1236
DpnII GATC 5 cut(s) 418, 721, 990, 1048, 1234
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 3 cut(s) 484, 779, 1138
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 1049
EarI CTCTTC 1 cut(s) 1049
Ecl136II GAGCTC 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 779
Eco130I CCWWGG 2 cut(s) 57, 472
Eco24I GRGCYC 1 cut(s) 73
Eco52I CGGCCG 1 cut(s) 779
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 1 cut(s) 615
Eco72I CACGTG 1 cut(s) 1154
EcoICRI GAGCTC 1 cut(s) 71
EcoT14I CCWWGG 2 cut(s) 57, 472
EcoT38I GRGCYC 1 cut(s) 73
ErhI CCWWGG 2 cut(s) 57, 472
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 7 cut(s) 89, 260, 365, 476, 564, 622, 656
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 3 cut(s) 39, 175, 752
FatI CATG 7 cut(s) 85, 256, 361, 472, 560, 618, 652
FblI GTMKAC 1 cut(s) 1167
Fnu4HI GCNGC 5 cut(s) 224, 309, 788, 791, 1031
FokI GGATG 3 cut(s) 124, 701, 854
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 5 cut(s) 224, 309, 788, 791, 1031
FspBI CTAG 3 cut(s) 39, 866, 1008
GluI GCNGC 5 cut(s) 224, 309, 788, 791, 1031
HaeIII GGCC 3 cut(s) 486, 781, 1140
HapII CCGG 1 cut(s) 1333
Hin1II CATG 7 cut(s) 89, 260, 365, 476, 564, 622, 656
HincII GTYRAC 2 cut(s) 672, 1168
HindII GTYRAC 2 cut(s) 672, 1168
HindIII AAGCTT 1 cut(s) 1279
HinfI GANTC 7 cut(s) 153, 163, 374, 500, 802, 1088, 1382
HpaII CCGG 1 cut(s) 1333
HphI GGTGA 2 cut(s) 447, 1391
Hpy166II GTNNAC 4 cut(s) 504, 672, 1168, 1201
Hpy188I TCNGA 6 cut(s) 75, 499, 595, 956, 1053, 1243
Hpy188III TCNNGA 9 cut(s) 24, 39, 141, 512, 659, 739, 965, 988, 1085
Hpy8I GTNNAC 4 cut(s) 504, 672, 1168, 1201
HpyCH4III ACNGT 3 cut(s) 508, 530, 928
HpyCH4IV ACGT 2 cut(s) 684, 1153
HpyCH4V TGCA 6 cut(s) 4, 194, 279, 467, 884, 1316
HpyF10VI GCNNNNNNNGC 2 cut(s) 787, 796
HpyF3I CTNAG 6 cut(s) 72, 498, 601, 760, 795, 953
HpySE526I ACGT 2 cut(s) 684, 1153
Hsp92II CATG 7 cut(s) 89, 260, 365, 476, 564, 622, 656
Kzo9I GATC 5 cut(s) 418, 721, 990, 1048, 1234
LmnI GCTCC 1 cut(s) 181
Lsp1109I GCAGC 5 cut(s) 210, 295, 774, 777, 1042
LweI GCATC 4 cut(s) 250, 454, 871, 929
MaeI CTAG 3 cut(s) 39, 866, 1008
MaeII ACGT 2 cut(s) 684, 1153
MaeIII GTNAC 5 cut(s) 61, 115, 187, 550, 1260
MalI GATC 5 cut(s) 420, 723, 992, 1050, 1236
MboI GATC 5 cut(s) 418, 721, 990, 1048, 1234
MflI RGATCY 1 cut(s) 1048
MhlI GDGCHC 1 cut(s) 73
MlsI TGGCCA 1 cut(s) 486
MluNI TGGCCA 1 cut(s) 486
MlyI GAGTC 4 cut(s) 147, 157, 509, 796
MmeI TCCRAC 2 cut(s) 615, 1267
Mox20I TGGCCA 1 cut(s) 486
MroXI GAANNNNTTC 2 cut(s) 34, 1299
MscI TGGCCA 1 cut(s) 486
MseI TTAA 5 cut(s) 203, 228, 266, 399, 909
MslI CAYNNNNRTG 3 cut(s) 90, 366, 477
Msp20I TGGCCA 1 cut(s) 486
MspI CCGG 1 cut(s) 1333
MspR9I CCNGG 1 cut(s) 1334
MwoI GCNNNNNNNGC 2 cut(s) 787, 796
NciI CCSGG 1 cut(s) 1334
NcoI CCATGG 1 cut(s) 472
NdeII GATC 5 cut(s) 418, 721, 990, 1048, 1234
NlaIII CATG 7 cut(s) 89, 260, 365, 476, 564, 622, 656
NlaIV GGNNCC 4 cut(s) 183, 663, 1050, 1193
NmeAIII GCCGAG 2 cut(s) 265, 1051
NspI RCATGY 2 cut(s) 365, 564
PciI ACATGT 1 cut(s) 560
PcsI WCGNNNNNNNCGW 1 cut(s) 1081
PdmI GAANNNNTTC 2 cut(s) 34, 1299
PfeI GAWTC 3 cut(s) 374, 1088, 1382
PfoI TCCNGGA 1 cut(s) 1332
PkrI GCNGC 5 cut(s) 225, 310, 789, 792, 1032
PleI GAGTC 4 cut(s) 147, 157, 508, 796
PmaCI CACGTG 1 cut(s) 1154
PmlI CACGTG 1 cut(s) 1154
PpsI GAGTC 4 cut(s) 147, 157, 508, 796
Ppu21I YACGTR 2 cut(s) 685, 1154
PscI ACATGT 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 73
PspCI CACGTG 1 cut(s) 1154
PspN4I GGNNCC 4 cut(s) 183, 663, 1050, 1193
PsrI GAACNNNNNNTAC 2 cut(s) 1284, 1316
PsuI RGATCY 1 cut(s) 1048
RsaI GTAC 4 cut(s) 208, 452, 683, 855
RsaNI GTAC 4 cut(s) 207, 451, 682, 854
RseI CAYNNNNRTG 3 cut(s) 90, 366, 477
SacI GAGCTC 1 cut(s) 73
SalI GTCGAC 1 cut(s) 1166
SaqAI TTAA 5 cut(s) 203, 228, 266, 399, 909
SatI GCNGC 5 cut(s) 224, 309, 788, 791, 1031
Sau3AI GATC 5 cut(s) 418, 721, 990, 1048, 1234
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 4 cut(s) 147, 157, 509, 796
ScrFI CCNGG 1 cut(s) 1334
SduI GDGCHC 1 cut(s) 73
SfaNI GCATC 4 cut(s) 250, 454, 871, 929
SmiMI CAYNNNNRTG 3 cut(s) 90, 366, 477
SsiI CCGC 1 cut(s) 1307
SspI AATATT 1 cut(s) 586
SspMI CTAG 3 cut(s) 39, 866, 1008
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 1332
StyI CCWWGG 2 cut(s) 57, 472
TaaI ACNGT 3 cut(s) 508, 530, 928
TaiI ACGT 2 cut(s) 687, 1156
TaqI TCGA 6 cut(s) 511, 805, 937, 987, 1167, 1303
TatI WGTACW 2 cut(s) 206, 450
TfiI GAWTC 3 cut(s) 374, 1088, 1382
Tru1I TTAA 5 cut(s) 203, 228, 266, 399, 909
Tru9I TTAA 5 cut(s) 203, 228, 266, 399, 909
TscAI CASTG 1 cut(s) 511
TseI GCWGC 5 cut(s) 223, 308, 787, 790, 1030
TspDTI ATGAA 4 cut(s) 102, 126, 635, 858
TspRI CASTG 1 cut(s) 511
XapI RAATTY 5 cut(s) 401, 625, 707, 904, 1322
XbaI TCTAGA 1 cut(s) 38
XceI RCATGY 2 cut(s) 365, 564
XcmI CCANNNNNNNNNTGG 2 cut(s) 479, 1202
XmiI GTMKAC 1 cut(s) 1167
XmnI GAANNNNTTC 2 cut(s) 34, 1299
XspI CTAG 3 cut(s) 39, 866, 1008
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.