FvH4_4g23810

HORMA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004206
Physical Location & Seq
Reverse (-)
26176966 .. 26182377
5412 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23810.t2

Sequence Viewer

Length: 1359 bp
ATGCAGACAAGGAAAAAAACTTCTGGAAGAACTGCTTCCAGAGAGCATACCAGTCCCAAGGTTACAAGAGCTCAGAAGAAAGCACATGAAAATGTCAGAGTATTGGAAACCAAAGTTACAGATATGATTACATCTTCCTCCAGGAAGCAGAGAGTCGGTGGAGCTCATAAGAAGAATGAGGACCCTGTTATTGCAAGCACTTTAAGTACAAATTATGACTTGCTGCTTAATGAGACTTCTGAGACTTGTTTGGGGCATGATGCTTTAAGTGATGATTGCAAAGAGAATAAGGGTTGTGTGGAGGGTACTAACAACTGCGTGCTGGGAACTATCTTTTCTCCTGGCTTTCACATACCGAAACATGCTGGTGGAGAATCTGCCAATGGAGTTGATTTTGTTAAATTCTTTCAAAATGGAGATCACACTTTCCATCAGGAGATGGAAATGAAGTACTCACCCGATGATGCACACCGTGGTTATGTTGGCCGAGAGACTTCTGAGTCCACTGTTCGAGACGAGAACTACTTTGTGGAGGCAACCAATATTTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAAAAATTCTAAGTTGGAATGTTTAGATGAGCATGGTCAGGAACCTATGTCAACTGATTTGTATGTGGATGAAGATGACAATGAGGAATTTGATGACTTTGATCCTTATTTATTTATCAAGAACTTACCGGATTTGTCCTCAGTCGTCCCAACTTTTCGGCCGTTGCTGCTGCCTAAACAGACTCGCAGTTGCCCTCCTACAAGTCTTGTTTTGGACTTGGATGAAACTTTGGTACATTCCACACTAGAACCTTGTGATGATGCAGACTTCACATTTCCCGTAAATTTTAACCTTCAAGAGCATACAGTATATGTTAGATGCCGTCCTCATCTCAGAGATTTTCTTGACAGAGTTTCGCTCCTTTTCGAGATCATTATCTTTACAGCCAGTCAGAGTATTTATGCAGAGCAGCTTCTAAATGTGTTGGATCCAAAGAGGAAGATTTTTCGCCATCGTGTTTTTCGTGATTCCTGTGTTTTTGTGGAGGGGAATTATCTCAAAGATTTGTCGGTTCTTGGCCGTGATTTGTCACGTGTTATCATAGTTGACAACTCTCCACAGGCATTTGGGTTTCAAGTGGACAATGGAATACCAATTGAGAGCTGGTTTGATGATCGTTCTGATACAGAATTACTTTCATTACTTCCTTTTTTGGAAAGCTTGGTTGGAGTAGAAGATGTTCGACCACTAATTGCAAACAAGTTCAACCTCCGGGAGAAAATAGCTACTGCTGAATATCCGCTCAACTCAAATAGAGGTGATTCGTTTATGCCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HORMA NIF REF

Protein Analysis

453

Amino Acids

51.18

Weight (kDa)

5.04

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 262 - 421 3.4e-60 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 499
AccBSI CCGCTC 1 cut(s) 1321
AciI CCGC 1 cut(s) 1319
AclWI GGATC 3 cut(s) 674, 1001, 1014
AcoI YGGCCR 3 cut(s) 484, 737, 1096
AcsI RAATTY 4 cut(s) 401, 583, 665, 862
AcuI CTGAAG 1 cut(s) 573
AcvI CACGTG 1 cut(s) 1112
AdeI CACNNNGTG 1 cut(s) 473
AfaI GTAC 4 cut(s) 208, 307, 452, 813
AflIII ACRYGT 1 cut(s) 1111
AgsI TTSAA 4 cut(s) 410, 875, 1154, 1285
AjnI CCWGG 2 cut(s) 140, 340
AjuI GAANNNNNNNTTGG 2 cut(s) 1227, 1259
AluBI AGCT 6 cut(s) 71, 164, 991, 1182, 1239, 1304
AluI AGCT 6 cut(s) 71, 164, 991, 1182, 1239, 1304
Alw21I GWGCWC 2 cut(s) 73, 166
Alw26I GTCTC 5 cut(s) 227, 236, 485, 507, 562
AlwI GGATC 3 cut(s) 674, 1001, 1014
AoxI GGCC 3 cut(s) 484, 737, 1096
ApeKI GCWGC 4 cut(s) 223, 745, 748, 988
ApoI RAATTY 4 cut(s) 401, 583, 665, 862
Asp700I GAANNNNTTC 2 cut(s) 34, 1257
AspS9I GGNCC 1 cut(s) 181
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 2 cut(s) 447, 1349
AvaII GGWCC 1 cut(s) 181
BamHI GGATCC 1 cut(s) 1006
BanII GRGCYC 2 cut(s) 73, 166
BbrPI CACGTG 1 cut(s) 1112
Bbv12I GWGCWC 2 cut(s) 73, 166
BbvI GCAGC 4 cut(s) 210, 732, 735, 1000
BccI CCATC 3 cut(s) 433, 438, 1038
BceAI ACGGC 3 cut(s) 724, 885, 1083
BciT130I CCWGG 2 cut(s) 142, 342
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 5 cut(s) 227, 236, 485, 507, 562
BfaI CTAG 1 cut(s) 824
BisI GCNGC 4 cut(s) 224, 746, 749, 989
BlsI GCNGC 4 cut(s) 225, 747, 750, 990
BmcAI AGTACT 1 cut(s) 452
Bme1390I CCNGG 3 cut(s) 142, 342, 1292
Bme18I GGWCC 1 cut(s) 181
BmgT120I GGNCC 1 cut(s) 181
BmiI GGNNCC 3 cut(s) 183, 621, 1008
BmrFI CCNGG 3 cut(s) 142, 342, 1292
BmsI GCATC 4 cut(s) 250, 454, 829, 887
BplI GAGNNNNNCTC 2 cut(s) 921, 953
BpmI CTGGAG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 1292
BsaAI YACGTR 1 cut(s) 1112
BsaBI GATNNNNATC 1 cut(s) 953
BsaJI CCNNGG 2 cut(s) 57, 472
BsaWI WCCGGW 1 cut(s) 706
Bse1I ACTGG 2 cut(s) 51, 966
Bse8I GATNNNNATC 1 cut(s) 953
BseBI CCWGG 2 cut(s) 142, 342
BseDI CCNNGG 2 cut(s) 57, 472
BseGI GGATG 2 cut(s) 652, 805
BseJI GATNNNNATC 1 cut(s) 953
BseMII CTCAG 6 cut(s) 86, 231, 489, 573, 732, 925
BseNI ACTGG 2 cut(s) 51, 966
BseX3I CGGCCG 1 cut(s) 737
BseXI GCAGC 4 cut(s) 210, 732, 735, 1000
BseYI CCCAGC 1 cut(s) 322
Bsh1285I CGRYCG 1 cut(s) 740
BshFI GGCC 3 cut(s) 486, 739, 1098
BsiEI CGRYCG 1 cut(s) 740
BsiHKAI GWGCWC 2 cut(s) 73, 166
BsiSI CCGG 2 cut(s) 707, 1291
BslFI GGGAC 2 cut(s) 39, 710
BsmAI GTCTC 5 cut(s) 227, 236, 485, 507, 562
BsmBI CGTCTC 1 cut(s) 507
BsmFI GGGAC 2 cut(s) 39, 710
BsnI GGCC 3 cut(s) 486, 739, 1098
Bsp1286I GDGCHC 2 cut(s) 73, 166
Bsp143I GATC 5 cut(s) 418, 679, 948, 1006, 1192
BspACI CCGC 1 cut(s) 1319
BspANI GGCC 3 cut(s) 486, 739, 1098
BspCNI CTCAG 6 cut(s) 85, 232, 490, 572, 731, 924
BspLI GGNNCC 3 cut(s) 183, 621, 1008
BspPI GGATC 3 cut(s) 674, 1001, 1014
BsrBI CCGCTC 1 cut(s) 1321
BsrI ACTGG 2 cut(s) 51, 966
BssECI CCNNGG 2 cut(s) 57, 472
BssMI GATC 5 cut(s) 418, 679, 948, 1006, 1192
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 2 cut(s) 142, 342
Bst4CI ACNGT 3 cut(s) 473, 508, 886
BstBAI YACGTR 1 cut(s) 1112
BstC8I GCNNGC 2 cut(s) 196, 320
BstDEI CTNAG 7 cut(s) 72, 240, 498, 559, 588, 718, 911
BstDSI CCRYGG 1 cut(s) 472
BstF5I GGATG 2 cut(s) 652, 805
BstKTI GATC 5 cut(s) 421, 682, 951, 1009, 1195
BstMAI GTCTC 5 cut(s) 227, 236, 485, 507, 562
BstMBI GATC 5 cut(s) 418, 679, 948, 1006, 1192
BstMCI CGRYCG 1 cut(s) 740
BstMWI GCNNNNNNNGC 1 cut(s) 745
BstNI CCWGG 2 cut(s) 142, 342
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 3 cut(s) 140, 340, 1290
BstV1I GCAGC 4 cut(s) 210, 732, 735, 1000
BstX2I RGATCY 1 cut(s) 1006
BstYI RGATCY 1 cut(s) 1006
BstZI CGGCCG 1 cut(s) 737
BsuRI GGCC 3 cut(s) 486, 739, 1098
BtgI CCRYGG 1 cut(s) 472
BtsCI GGATG 2 cut(s) 652, 805
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 196, 320
Cfr13I GGNCC 1 cut(s) 181
Csp6I GTAC 4 cut(s) 207, 306, 451, 812
CviAII CATG 5 cut(s) 86, 257, 362, 577, 611
CviQI GTAC 4 cut(s) 207, 306, 451, 812
DdeI CTNAG 7 cut(s) 72, 240, 498, 559, 588, 718, 911
DpnI GATC 5 cut(s) 420, 681, 950, 1008, 1194
DpnII GATC 5 cut(s) 418, 679, 948, 1006, 1192
DraIII CACNNNGTG 1 cut(s) 473
DrdI GACNNNNNNGTC 1 cut(s) 499
DseDI GACNNNNNNGTC 1 cut(s) 499
EaeI YGGCCR 3 cut(s) 484, 737, 1096
EagI CGGCCG 1 cut(s) 737
Ecl136II GAGCTC 2 cut(s) 71, 164
EclXI CGGCCG 1 cut(s) 737
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 2 cut(s) 73, 166
Eco47I GGWCC 1 cut(s) 181
Eco52I CGGCCG 1 cut(s) 737
Eco53kI GAGCTC 2 cut(s) 71, 164
Eco57I CTGAAG 1 cut(s) 573
Eco72I CACGTG 1 cut(s) 1112
EcoICRI GAGCTC 2 cut(s) 71, 164
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 140, 340
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 2 cut(s) 73, 166
ErhI CCWWGG 1 cut(s) 57
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 5 cut(s) 89, 260, 365, 580, 614
FalI AAGNNNNNCTT 2 cut(s) 19, 51
FaqI GGGAC 2 cut(s) 39, 710
FatI CATG 5 cut(s) 85, 256, 361, 576, 610
Fnu4HI GCNGC 4 cut(s) 224, 746, 749, 989
FokI GGATG 2 cut(s) 659, 812
FriOI GRGCYC 2 cut(s) 73, 166
Fsp4HI GCNGC 4 cut(s) 224, 746, 749, 989
FspBI CTAG 1 cut(s) 824
GluI GCNGC 4 cut(s) 224, 746, 749, 989
GsaI CCCAGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 124
HaeIII GGCC 3 cut(s) 486, 739, 1098
HapII CCGG 2 cut(s) 707, 1291
Hin1II CATG 5 cut(s) 89, 260, 365, 580, 614
HincII GTYRAC 2 cut(s) 630, 1126
HindII GTYRAC 2 cut(s) 630, 1126
HindIII AAGCTT 1 cut(s) 1237
HinfI GANTC 6 cut(s) 153, 374, 500, 760, 1046, 1340
HpaII CCGG 2 cut(s) 707, 1291
HphI GGTGA 2 cut(s) 447, 1349
Hpy166II GTNNAC 4 cut(s) 504, 630, 1126, 1159
Hpy188I TCNGA 8 cut(s) 75, 98, 241, 499, 553, 914, 972, 1201
Hpy8I GTNNAC 4 cut(s) 504, 630, 1126, 1159
HpyAV CCTTC 1 cut(s) 881
HpyCH4III ACNGT 3 cut(s) 473, 508, 886
HpyCH4IV ACGT 1 cut(s) 1111
HpyCH4V TGCA 7 cut(s) 4, 194, 279, 467, 842, 983, 1274
HpyF10VI GCNNNNNNNGC 1 cut(s) 745
HpyF3I CTNAG 7 cut(s) 72, 240, 498, 559, 588, 718, 911
HpySE526I ACGT 1 cut(s) 1111
Hsp92II CATG 5 cut(s) 89, 260, 365, 580, 614
Kzo9I GATC 5 cut(s) 418, 679, 948, 1006, 1192
LmnI GCTCC 2 cut(s) 161, 942
Lsp1109I GCAGC 4 cut(s) 210, 732, 735, 1000
LweI GCATC 4 cut(s) 250, 454, 829, 887
MaeI CTAG 1 cut(s) 824
MaeII ACGT 1 cut(s) 1111
MaeIII GTNAC 3 cut(s) 61, 115, 1107
MalI GATC 5 cut(s) 420, 681, 950, 1008, 1194
MbiI CCGCTC 1 cut(s) 1321
MboI GATC 5 cut(s) 418, 679, 948, 1006, 1192
MboII GAAGA 8 cut(s) 39, 88, 126, 184, 540, 662, 1030, 1265
MfeI CAATTG 1 cut(s) 1173
MflI RGATCY 1 cut(s) 1006
MhlI GDGCHC 2 cut(s) 73, 166
MluCI AATT 9 cut(s) 211, 401, 583, 665, 862, 1069, 1173, 1208, 1269
MlyI GAGTC 3 cut(s) 162, 509, 754
MmeI TCCRAC 3 cut(s) 573, 984, 1225
MroXI GAANNNNTTC 2 cut(s) 34, 1257
MseI TTAA 5 cut(s) 203, 228, 266, 399, 867
MslI CAYNNNNRTG 2 cut(s) 90, 366
MspI CCGG 2 cut(s) 707, 1291
MspR9I CCNGG 3 cut(s) 142, 342, 1292
MunI CAATTG 1 cut(s) 1173
MvaI CCWGG 2 cut(s) 142, 342
MwoI GCNNNNNNNGC 1 cut(s) 745
NciI CCSGG 1 cut(s) 1292
NdeII GATC 5 cut(s) 418, 679, 948, 1006, 1192
NlaIII CATG 5 cut(s) 89, 260, 365, 580, 614
NlaIV GGNNCC 3 cut(s) 183, 621, 1008
NmeAIII GCCGAG 1 cut(s) 512
NmuCI GTSAC 1 cut(s) 1107
NspI RCATGY 1 cut(s) 365
PcsI WCGNNNNNNNCGW 1 cut(s) 1039
PdmI GAANNNNTTC 2 cut(s) 34, 1257
PfeI GAWTC 3 cut(s) 374, 1046, 1340
PfoI TCCNGGA 2 cut(s) 140, 1290
PkrI GCNGC 4 cut(s) 225, 747, 750, 990
PleI GAGTC 3 cut(s) 161, 508, 754
PmaCI CACGTG 1 cut(s) 1112
PmlI CACGTG 1 cut(s) 1112
PpsI GAGTC 3 cut(s) 161, 508, 754
Ppu21I YACGTR 1 cut(s) 1112
PpuMI RGGWCCY 1 cut(s) 181
Psp124BI GAGCTC 2 cut(s) 73, 166
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 140, 340
PspCI CACGTG 1 cut(s) 1112
PspFI CCCAGC 1 cut(s) 322
PspGI CCWGG 2 cut(s) 140, 340
PspN4I GGNNCC 3 cut(s) 183, 621, 1008
PspPI GGNCC 1 cut(s) 181
PspPPI RGGWCCY 1 cut(s) 181
PsrI GAACNNNNNNTAC 2 cut(s) 1242, 1274
PsuI RGATCY 1 cut(s) 1006
RsaI GTAC 4 cut(s) 208, 307, 452, 813
RsaNI GTAC 4 cut(s) 207, 306, 451, 812
RseI CAYNNNNRTG 2 cut(s) 90, 366
SacI GAGCTC 2 cut(s) 73, 166
SaqAI TTAA 5 cut(s) 203, 228, 266, 399, 867
SatI GCNGC 4 cut(s) 224, 746, 749, 989
Sau3AI GATC 5 cut(s) 418, 679, 948, 1006, 1192
Sau96I GGNCC 1 cut(s) 181
ScaI AGTACT 1 cut(s) 452
SchI GAGTC 3 cut(s) 162, 509, 754
ScrFI CCNGG 3 cut(s) 142, 342, 1292
SduI GDGCHC 2 cut(s) 73, 166
SfaNI GCATC 4 cut(s) 250, 454, 829, 887
SinI GGWCC 1 cut(s) 181
SmiMI CAYNNNNRTG 2 cut(s) 90, 366
Sse9I AATT 9 cut(s) 211, 401, 583, 665, 862, 1069, 1173, 1208, 1269
SsiI CCGC 1 cut(s) 1319
SspI AATATT 1 cut(s) 544
SspMI CTAG 1 cut(s) 824
SstI GAGCTC 2 cut(s) 73, 166
StyD4I CCNGG 3 cut(s) 140, 340, 1290
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 3 cut(s) 473, 508, 886
TaiI ACGT 1 cut(s) 1114
TaqI TCGA 3 cut(s) 511, 945, 1261
TasI AATT 9 cut(s) 211, 401, 583, 665, 862, 1069, 1173, 1208, 1269
TatI WGTACW 2 cut(s) 206, 450
TfiI GAWTC 3 cut(s) 374, 1046, 1340
Tru1I TTAA 5 cut(s) 203, 228, 266, 399, 867
Tru9I TTAA 5 cut(s) 203, 228, 266, 399, 867
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 1107
TseI GCWGC 4 cut(s) 223, 745, 748, 988
Tsp45I GTSAC 1 cut(s) 1107
TspDTI ATGAA 6 cut(s) 102, 461, 593, 663, 816, 1206
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 181
XapI RAATTY 4 cut(s) 401, 583, 665, 862
XceI RCATGY 1 cut(s) 365
XcmI CCANNNNNNNNNTGG 1 cut(s) 1179
XmnI GAANNNNTTC 2 cut(s) 34, 1257
XspI CTAG 1 cut(s) 824
ZrmI AGTACT 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.