MD16G1118800.v1.1

CTD small phosphatase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004206
Physical Location & Seq
Forward (+)
8472259 .. 8476744
4486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1118800.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGCAAACGAAGAAAAGAAGTTCTGGAAGAACTGCTGCGCGAGAGCATGCTAGCCCCAAGGTCACAAGAGCTCAGAAGAAAGCGTATGAAAGTGTGCAAGTTGTGGAAAAGAAAGTTGCAGACCTAATTACATCTTCAGCAAGGAAGCAGAGAATACGTGGCACTCATAAAAAGAACGGGGGTGCTGTTACTGCAACCAATTCAGATTTGAATTATGATTCGATGCGTGATGAGACTGCTGACACTTGTTTGGGGCATGATGCTATGAGTGATGATTGCATAGAGATTAAGGATTGTATAGAGGGAACTGCTGATTGCACAGCAGAAACAATATTTTCTCCTGCCTTTCACATATCCAAACATGCCGAAGGTGAATCTGATAATGGAGTTGATTTTGTTAACTTCTATAGAAATGGAGACCACAATTTTCATCAAGATTGTGAAATAATGTACTCAAGGGTTGATGTGCTGAATGGTCATGTTGTTCAAGAGACTTCCGAATCCATTGTTAGAGGCGAGAGGAGTTACTGTGAGAACTCATTTTCTTCAGTAAATATAACATATCCTGTTGTGGAGCCAGCCATTATCTCTTCTGAAGTCTCAGCCATATATCTTGCCATGAGAAATTCCAAGTTGGAATGTGTGGATGAGCATGGTCAGGAGCCCATGTCAACTGATTTATACACAGATGATGACGAGAATGAGGAAGTTGATGACTTTGATCCTTACTTCTTTATCAAGAACTTACCTGACTTGTCAGCAGTTGTTCCAACTTTTCGGCCGATGCTTTTACCTAAACAGACACGGCGTTGTCCTCCTACTAGTCTTGTTTTGGACTTGGATGAAACTTTGGTACACTCCACACTAGAACCTTGTGATGATGCAGACTTCACTTTCCCCGTTAATTTTAACCTCCAAGAGCACACAGTATATGTCCGATGCCGTCCTCATCTCAGAGATTTTTTGGACAGAGTTTCCAATCTTTTTGAGATCATAATATTTACAGCTAGTCAGAGTATTTATGCAGAGCAACTTCTAAACGTGCTGGACCCAAAAAGGAAGATATTTCGCCATCGTGTTTTTCGGGATTCGTGTGTTTTTGTGGAGGGGAATTACCTCAAAGATTTGTCAGTTCTTGGTCGTGATTTGGCACACACTATCATAATTGACAACTCTCCACAGGCATTCGGGTTCCAAGTAGACAATGGAATACCAATCGAGAGCTGGTTTGATGATCATTCCGATAAAGAGTTACTCTCCTTACTTCCTTTTTTGGAGAGCCTGGTCGGAGTAGAAGATGTTCGGCCACTAATTGCAAGCAAATTCAACCTTAGGGAGAAAATAGCTGCTGCTGTTTATCCTTTTAGGTCAAACAGAGGCGATCCTCTCGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

466

Amino Acids

52.65

Weight (kDa)

5.01

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 276 - 435 1e-59 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1200
AccII CGCG 1 cut(s) 40
AclWI GGATC 2 cut(s) 716, 1376
AcoI YGGCCR 2 cut(s) 779, 1304
AcsI RAATTY 2 cut(s) 625, 1322
AcuI CTGAAG 3 cut(s) 120, 531, 615
AfaI GTAC 2 cut(s) 452, 855
AgsI TTSAA 3 cut(s) 211, 488, 1327
AhlI ACTAGT 1 cut(s) 821
AjnI CCWGG 1 cut(s) 1281
AluBI AGCT 4 cut(s) 71, 1007, 1224, 1346
AluI AGCT 4 cut(s) 71, 1007, 1224, 1346
Alw21I GWGCWC 2 cut(s) 73, 924
Alw26I GTCTC 4 cut(s) 227, 411, 485, 604
AlwI GGATC 2 cut(s) 716, 1376
AoxI GGCC 2 cut(s) 779, 1304
ApeKI GCWGC 3 cut(s) 35, 1346, 1349
ApoI RAATTY 2 cut(s) 625, 1322
Asp700I GAANNNNTTC 1 cut(s) 1299
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 1 cut(s) 1048
AsuHPI GGTGA 1 cut(s) 383
AsuNHI GCTAGC 1 cut(s) 50
AvaII GGWCC 1 cut(s) 1048
AxyI CCTNAGG 1 cut(s) 1331
BanII GRGCYC 2 cut(s) 73, 666
Bbv12I GWGCWC 2 cut(s) 73, 924
BbvI GCAGC 3 cut(s) 22, 1333, 1336
BccI CCATC 1 cut(s) 1080
BceAI ACGGC 2 cut(s) 821, 927
BciT130I CCWGG 1 cut(s) 1283
BclI TGATCA 1 cut(s) 1234
BcoDI GTCTC 4 cut(s) 227, 411, 485, 604
BcuI ACTAGT 1 cut(s) 821
BfaI CTAG 4 cut(s) 51, 822, 866, 1008
BfmI CTRYAG 1 cut(s) 406
BisI GCNGC 3 cut(s) 36, 1347, 1350
BlsI GCNGC 3 cut(s) 37, 1348, 1351
Bme1390I CCNGG 1 cut(s) 1283
Bme18I GGWCC 1 cut(s) 1048
BmgT120I GGNCC 1 cut(s) 1048
BmiI GGNNCC 4 cut(s) 576, 663, 1050, 1193
BmrFI CCNGG 1 cut(s) 1283
BmsI GCATC 5 cut(s) 213, 250, 774, 871, 929
BmtI GCTAGC 1 cut(s) 54
BplI GAGNNNNNCTC 2 cut(s) 1241, 1273
BpuEI CTTGAG 1 cut(s) 439
BsaAI YACGTR 1 cut(s) 158
BsaI GGTCTC 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 57
BsaXI ACNNNNNCTCC 4 cut(s) 514, 544, 653, 683
Bse21I CCTNAGG 1 cut(s) 1331
BseBI CCWGG 1 cut(s) 1283
BseDI CCNNGG 1 cut(s) 57
BseGI GGATG 2 cut(s) 652, 847
BseMII CTCAG 3 cut(s) 86, 615, 967
BseRI GAGGAG 1 cut(s) 535
BseX3I CGGCCG 1 cut(s) 779
BseXI GCAGC 3 cut(s) 22, 1333, 1336
Bsh1236I CGCG 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 782
BshFI GGCC 2 cut(s) 781, 1306
BsiEI CGRYCG 1 cut(s) 782
BsiHKAI GWGCWC 2 cut(s) 73, 924
BsmAI GTCTC 4 cut(s) 227, 411, 485, 604
BsmI GAATGC 1 cut(s) 1184
BsnI GGCC 2 cut(s) 781, 1306
Bso31I GGTCTC 1 cut(s) 411
Bsp1286I GDGCHC 3 cut(s) 73, 666, 924
Bsp143I GATC 4 cut(s) 721, 990, 1234, 1381
BspANI GGCC 2 cut(s) 781, 1306
BspCNI CTCAG 3 cut(s) 85, 614, 966
BspFNI CGCG 1 cut(s) 40
BspLI GGNNCC 4 cut(s) 576, 663, 1050, 1193
BspOI GCTAGC 1 cut(s) 54
BspPI GGATC 2 cut(s) 716, 1376
BspTNI GGTCTC 1 cut(s) 411
BssECI CCNNGG 1 cut(s) 57
BssMI GATC 4 cut(s) 721, 990, 1234, 1381
BssT1I CCWWGG 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 1283
Bst4CI ACNGT 2 cut(s) 530, 928
Bst6I CTCTTC 1 cut(s) 595
BstBAI YACGTR 1 cut(s) 158
BstC8I GCNNGC 4 cut(s) 48, 52, 579, 1318
BstDEI CTNAG 4 cut(s) 72, 601, 953, 1331
BstF5I GGATG 2 cut(s) 652, 847
BstFNI CGCG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 40
BstKTI GATC 4 cut(s) 724, 993, 1237, 1384
BstMAI GTCTC 4 cut(s) 227, 411, 485, 604
BstMBI GATC 4 cut(s) 721, 990, 1234, 1381
BstMCI CGRYCG 1 cut(s) 782
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 1 cut(s) 1283
BstNSI RCATGY 2 cut(s) 50, 365
BstSCI CCNGG 1 cut(s) 1281
BstSFI CTRYAG 1 cut(s) 406
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 3 cut(s) 22, 1333, 1336
BstZI CGGCCG 1 cut(s) 779
Bsu36I CCTNAGG 1 cut(s) 1331
BsuRI GGCC 2 cut(s) 781, 1306
BtsCI GGATG 2 cut(s) 652, 847
Cac8I GCNNGC 4 cut(s) 48, 52, 579, 1318
CfoI GCGC 1 cut(s) 40
Cfr13I GGNCC 1 cut(s) 1048
Csp6I GTAC 2 cut(s) 451, 854
CviAII CATG 7 cut(s) 47, 257, 362, 479, 619, 653, 667
CviQI GTAC 2 cut(s) 451, 854
DdeI CTNAG 4 cut(s) 72, 601, 953, 1331
DpnI GATC 4 cut(s) 723, 992, 1236, 1383
DpnII GATC 4 cut(s) 721, 990, 1234, 1381
EaeI YGGCCR 2 cut(s) 779, 1304
EagI CGGCCG 1 cut(s) 779
Eam1104I CTCTTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 595
Ecl136II GAGCTC 1 cut(s) 71
EclXI CGGCCG 1 cut(s) 779
Eco130I CCWWGG 1 cut(s) 57
Eco24I GRGCYC 2 cut(s) 73, 666
Eco31I GGTCTC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 1048
Eco52I CGGCCG 1 cut(s) 779
Eco53kI GAGCTC 1 cut(s) 71
Eco57I CTGAAG 3 cut(s) 120, 531, 615
Eco81I CCTNAGG 1 cut(s) 1331
EcoICRI GAGCTC 1 cut(s) 71
EcoRII CCWGG 1 cut(s) 1281
EcoT14I CCWWGG 1 cut(s) 57
EcoT38I GRGCYC 2 cut(s) 73, 666
ErhI CCWWGG 1 cut(s) 57
FaeI CATG 7 cut(s) 50, 260, 365, 482, 622, 656, 670
FatI CATG 7 cut(s) 46, 256, 361, 478, 618, 652, 666
FbaI TGATCA 1 cut(s) 1234
FblI GTMKAC 1 cut(s) 1200
Fnu4HI GCNGC 3 cut(s) 36, 1347, 1350
FokI GGATG 2 cut(s) 659, 854
FriOI GRGCYC 2 cut(s) 73, 666
Fsp4HI GCNGC 3 cut(s) 36, 1347, 1350
FspBI CTAG 4 cut(s) 51, 822, 866, 1008
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 36, 1347, 1350
HaeIII GGCC 2 cut(s) 781, 1306
HhaI GCGC 1 cut(s) 40
Hin1II CATG 7 cut(s) 50, 260, 365, 482, 622, 656, 670
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HincII GTYRAC 2 cut(s) 400, 672
HindII GTYRAC 2 cut(s) 400, 672
HinfI GANTC 4 cut(s) 218, 374, 500, 1088
HpaI GTTAAC 1 cut(s) 400
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 4 cut(s) 400, 672, 856, 1201
Hpy188III TCNNGA 9 cut(s) 24, 434, 488, 659, 739, 1085, 1142, 1219, 1388
Hpy8I GTNNAC 4 cut(s) 400, 672, 856, 1201
HpyAV CCTTC 1 cut(s) 362
HpyCH4III ACNGT 2 cut(s) 530, 928
HpyCH4IV ACGT 2 cut(s) 157, 1041
HpyCH4V TGCA 9 cut(s) 4, 97, 119, 194, 279, 318, 884, 1025, 1316
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 4 cut(s) 72, 601, 953, 1331
HpySE526I ACGT 2 cut(s) 157, 1041
Hsp92II CATG 7 cut(s) 50, 260, 365, 482, 622, 656, 670
HspAI GCGC 1 cut(s) 38
Ksp22I TGATCA 1 cut(s) 1234
KspAI GTTAAC 1 cut(s) 400
Kzo9I GATC 4 cut(s) 721, 990, 1234, 1381
LmnI GCTCC 2 cut(s) 574, 661
Lsp1109I GCAGC 3 cut(s) 22, 1333, 1336
LweI GCATC 5 cut(s) 213, 250, 774, 871, 929
MaeI CTAG 4 cut(s) 51, 822, 866, 1008
MaeII ACGT 2 cut(s) 157, 1041
MaeIII GTNAC 4 cut(s) 61, 187, 524, 1251
MalI GATC 4 cut(s) 723, 992, 1236, 1383
MboI GATC 4 cut(s) 721, 990, 1234, 1381
MboII GAAGA 8 cut(s) 22, 39, 88, 126, 537, 582, 1072, 1307
MhlI GDGCHC 3 cut(s) 73, 666, 924
MmeI TCCRAC 3 cut(s) 615, 794, 1267
MroXI GAANNNNTTC 1 cut(s) 1299
MseI TTAA 4 cut(s) 288, 399, 903, 909
MspR9I CCNGG 1 cut(s) 1283
Mva1269I GAATGC 1 cut(s) 1184
MvaI CCWGG 1 cut(s) 1283
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 4 cut(s) 721, 990, 1234, 1381
NheI GCTAGC 1 cut(s) 50
NlaIII CATG 7 cut(s) 50, 260, 365, 482, 622, 656, 670
NlaIV GGNNCC 4 cut(s) 576, 663, 1050, 1193
NmuCI GTSAC 1 cut(s) 61
NspI RCATGY 2 cut(s) 50, 365
PaeI GCATGC 1 cut(s) 50
PctI GAATGC 1 cut(s) 1184
PdmI GAANNNNTTC 1 cut(s) 1299
PfeI GAWTC 4 cut(s) 218, 374, 500, 1088
PkrI GCNGC 3 cut(s) 37, 1348, 1351
Ppu21I YACGTR 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 73
Psp6I CCWGG 1 cut(s) 1281
PspGI CCWGG 1 cut(s) 1281
PspN4I GGNNCC 4 cut(s) 576, 663, 1050, 1193
PspPI GGNCC 1 cut(s) 1048
PsrI GAACNNNNNNTAC 2 cut(s) 1284, 1316
RsaI GTAC 2 cut(s) 452, 855
RsaNI GTAC 2 cut(s) 451, 854
SacI GAGCTC 1 cut(s) 73
SaqAI TTAA 4 cut(s) 288, 399, 903, 909
SatI GCNGC 3 cut(s) 36, 1347, 1350
Sau3AI GATC 4 cut(s) 721, 990, 1234, 1381
Sau96I GGNCC 1 cut(s) 1048
ScrFI CCNGG 1 cut(s) 1283
SduI GDGCHC 3 cut(s) 73, 666, 924
SfaNI GCATC 5 cut(s) 213, 250, 774, 871, 929
SfcI CTRYAG 1 cut(s) 406
SinI GGWCC 1 cut(s) 1048
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
SpeI ACTAGT 1 cut(s) 821
SphI GCATGC 1 cut(s) 50
SspI AATATT 2 cut(s) 333, 999
SspMI CTAG 4 cut(s) 51, 822, 866, 1008
SstI GAGCTC 1 cut(s) 73
StyD4I CCNGG 1 cut(s) 1281
StyI CCWWGG 1 cut(s) 57
TaaI ACNGT 2 cut(s) 530, 928
TaiI ACGT 2 cut(s) 160, 1044
TaqI TCGA 3 cut(s) 221, 1218, 1389
TatI WGTACW 1 cut(s) 450
TfiI GAWTC 4 cut(s) 218, 374, 500, 1088
Tru1I TTAA 4 cut(s) 288, 399, 903, 909
Tru9I TTAA 4 cut(s) 288, 399, 903, 909
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 3 cut(s) 35, 1346, 1349
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 3 cut(s) 102, 419, 858
VpaK11BI GGWCC 1 cut(s) 1048
XapI RAATTY 2 cut(s) 625, 1322
XceI RCATGY 2 cut(s) 50, 365
XcmI CCANNNNNNNNNTGG 2 cut(s) 1202, 1221
XmiI GTMKAC 1 cut(s) 1200
XmnI GAANNNNTTC 1 cut(s) 1299
XspI CTAG 4 cut(s) 51, 822, 866, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.