AT5G13000

ATGSL12,gsl12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005959
Physical Location & Seq
Reverse (-)
4110171 .. 4121489
11319 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13000.2

Sequence Viewer

Length: 5868 bp
ATGTCTGCTACGAGAGGAGGTCCTGATCAAGGGCCGTCTCAGCCTCAGCAGCGGCGGATTATACGAACTCAGACTGCTGGTAATCTTGGAGAGTCATTCGATAGTGAAGTTGTTCCATCATCTCTTGTTGAGATTGCCCCCATTCTTCGGGTTGCTAATGAAGTTGAATCTAGTAATCCTAGGGTTGCGTATCTCTGTCGGTTTTATGCATTTGAGAAAGCACATAGATTGGATCCTACCTCCAGTGGAAGAGGTGTTCGGCAGTTTAAGACTGCACTTCTACAGCGTCTTGAAAGAGAACATGATCCAACACTGATGGGCAGGGTTAAGAAAAGTGATGCCCGTGAAATGCAAAGCTTTTATCAACACTACTATAAGAAGTATATTCAAGCTTTGCACAATGCTGCTGATAAGGCTGACCGTGCCCAGCTGACAAAGGCATACCAAACTGCCAATGTTTTGTTTGAGGTGTTGAAGGCTGTTAATCTGACACAGTCCATTGAGGTTGACCGGGAGATTCTGGAAGCTCAAGATAAGGTTGCAGAAAAGACACAGTTGTATGTCCCCTATAATATCCTACCTCTCGATCCTGATAGTGCCAATCAAGCGATTATGAGATATCCTGAGATCCAAGCTGCTGTTCTTGCTCTCCGCAATACTAGAGGTCTTCCTTGGCCAGAAGGTCACAAGAAAAAGAAAGATGAAGACATGCTTGATTGGCTTCAAGAAATGTTTGGATTTCAGAAAGACAATGTGGCTAATCAAAGGGAGCATCTGATCTTATTACTTGCTAATGTGCATATAAGACAATTTCCTAAGCCTGATCAGCAGCCAAAGTTAGATGATCAAGCATTGACAGAAGTGATGAAGAAGCTTTTCAAGAACTATAAGAAATGGTGCAAATACCTTGGTCGAAAGAGTAGTCTTTGGTTGCCAACTATACAGCAGGAAATGCAGCAGCGCAAACTATTGTATATGGCCCTGTATCTTCTAATTTGGGGTGAAGCAGCGAACTTGAGATTCATGCCAGAGTGTCTCTGCTACATTTATCATCATATGGCATTTGAACTCTATGGTATGCTGGCTGGGAATGTTAGTCCTATGACTGGGGAAAATGTGAAGCCAGCTTATGGAGGCGAGGAAGATGCATTCTTGAGGAAAGTTGTGACTCCTATTTATGAAGTGATTCAGATGGAGGCTCAAAGAAGCAAAAAAGGGAAGTCTAAGCACTCTCAGTGGAGGAACTACGATGATTTGAATGAATACTTTTGGTCAGTTGATTGTTTTCGGTTAGGTTGGCCAATGCGAGCTGATGCTGATTTCTTTTGTCTGCCTGTTGCTGTACCCAATACAGAAAAGGACGGGGATAACAGCAAGCCTATTGTCGCCAGAGATAGATGGGTTGGTAAAGTTAATTTTGTTGAGATTCGTTCTTTCTGGCATGTCTTCAGAAGTTTCGATCGAATGTGGAGCTTCTATATTCTGTGCCTCCAGGCCATGATTATTATGGCCTGGGATGGCGGACAACCAAGTTCAGTCTTTGGAGCTGATGTATTCAAGAAAGTTCTGAGCGTGTTTATCACAGCTGCAATAATGAAACTTGGACAAGCTGTTCTTGATGTGATCCTTAATTTCAAAGCTCATCAAAGCATGACACTACATGTTAAACTAAGATACATTCTGAAAGTGTTCTCTGCTGCTGCCTGGGTTATTATTCTGCCTGTTACTTACGCCTACAGCTGGAAAGATCCTCCAGCATTTGCCAGAACTATCAAGAGTTGGTTTGGGAGTGCTATGCACTCACCTTCTCTATTTATAATAGCAGTCGTTTCCTATTTGTCACCTAACATGCTCGCGGGTGTAATGTTTCTTTTCCCTCTGCTACGACGGTTTCTTGAGAGGTCCAACTACAGAATTGTAATGCTAATGATGTGGTGGTCTCAGCCCAGACTCTACGTTGGCAGAGGAATGCACGAGAGCGCATTTTCCCTCTTCAAATATACCATGTTCTGGGTGTTGCTTATTGCAACAAAGCTGGCATTCAGTTACTACATTGAGATCAGGCCTTTAGTTGCCCCAACACAAGCCATTATGAAGGCCCGTGTGACAAATTTTCAGTGGCATGAGTTCTTTCCTCGTGCCAAAAACAATATTGGTGTCGTTATCGCCCTCTGGGCCCCCATTATTCTGGTATATTTTATGGATAGTCAGATTTGGTATGCTATATTTTCCACATTATTTGGAGGTATCTACGGTGCATTCCGCCGCCTTGGAGAGATTCGGACACTGGGAATGCTCAGATCACGGTTTGAATCACTGCCTGGAGCTTTTAATGACCGCTTAATTCCAGATGGAAAGAACCAGCAAAAGAAGAAGGGAATAAGGGCAACGTTATCTCATAACTTTACGGAGGATAAGGTACCTGTGAACAAAGAGAAAGAAGCTGCAAGATTTGCACAGTTGTGGAACACAATAATTAGTAGTTTTAGAGAGGAAGATCTTATAAGTGATAGGGAGATGGATCTGTTGCTTGTTCCTTATTGGGCTGACCGTGATTTGGATCTCATACAGTGGCCTCCCTTCTTATTGGCTAGCAAGATTCCAATAGCATTAGATATGGCAAAAGACAGTAATGGGAAGGACAGAGAGCTCAAGAAGAGGATTGAGAGTGACACTTACATGAAATGTGCTGTCCGTGAATGCTATGCTTCATTCAAGAATATCATAAAATTTGTGGTTCAGGGAAACCGTGAAAAAGAGGTGATAGAGATCATATTTGCAGAGGTTGACAAACATATAGATACAGGGGATTTGATTCAAGAATACAAGATGAGTGCTCTTCCTAGCCTCTATGATCACTTTGTCAAGTTGATCAAATATTTGCTTGACAATAAGGAGGAAGATAGAGATCACGTTGTAATTCTCTTCCAAGACATGCTGGAAGTTGTAACAAGAGACATTATGATGGAGGATTACAATATATCGAGCTTGGTAGATTCAAGTCATGGTGGCACTTGGCATGGGGGAATGATCCCTCTCGAACAACAATATCAGCTGTTTGCATCCTCAGGTGCCATTAGATTTCCTATTGAACCAGTAACAGAAGCTTGGAAAGAGAAGATCAAACGGATATATCTTTTGTTGACTACTAAAGAGTCTGCTATGGATGTCCCCTCTAACTTGGAAGCAAGAAGGCGGATCTCATTCTTCTCAAATTCGTTGTTCATGGACATGCCTATGGCACCTAAAGTTCGCAACATGCTATCATTTTCTGTTTTGACTCCTTATTACACTGAAGAGGTCCTCTTCTCCTTGCGGGACCTGGAAACACCAAATGAAGATGGTGTTTCAATCCTCTTCTACCTACAAAAGATTTTTCCAGATGAATGGAACAATTTTCTTGAGCGGGTGAAGTGCTTAAGTGAAGAGGAACTTAAGGAGTCTGATGAATTAGAGGAGGAACTTCGTTTATGGGCTTCATATAGAGGGCAAACTTTGACTAGAACTGTAAGAGGAATGATGTACTATCGAAAAGCCTTGGAGCTTCAGGCATTCCTTGACATGGCTATGCATGAAGATTTGATGGAAGGTTACAAGGCTGTAGAACTAAATTCAGAGAACAATTCAAGAGGTGAAAGATCACTCTGGGCACAGTGCCAAGCTGTTGCTGACATGAAGTTTACATATGTTGTATCATGTCAACAATATGGTATCCATAAACGATCCGGTGATCCTCGTGCGCAGGATATATTGAGGCTTATGACAAGATACCCTTCACTCCGTGTTGCATATATTGATGAGGTCGAAGAACCAGTGAAAGACAAGTCAAAGAAAGGGAACCAGAAAGTGTATTATTCTGTGTTGGTAAAGGTGCCTAAGTCAACTGATCATTCTACCCTAGCACAAAATCTGGACCAGGTTATCTACAGGATTAGGCTTCCTGGACCTGCTATTCTTGGAGAAGGAAAGCCAGAAAACCAAAATCATGCTATCATTTTTTCCCGTGGAGAAGGTTTACAGACAATCGATATGAATCAGGATAATTACATGGAGGAAGCGTTAAAGATGAGGAATTTGCTTCAAGAATTTCTTACAAAACATGATGGTGTTAGACATCCATCCATTCTTGGACTTAGGGAACATATATTTACTGGATCAGTTTCATCCCTTGCTTGGTTCATGTCAAATCAAGAGACTAGTTTTGTTACCATTGGTCAGAGATTATTGGCAAATCCATTGAGGGTGCGTTTCCATTATGGCCATCCAGATGTTTTTGATAGACTGTTTCATCTAACAAGAGGTGGTGTTAGCAAAGCATCCAAGGTAATCAACCTCAGTGAAGATATATTTGCAGGATTCAATTCTACACTTCGTGAAGGAAATGTTACCCATCATGAATACATACAAGTTGGTAAAGGAAGAGATGTTGGTCTAAATCAGATCTCTATGTTTGAGGCCAAGATTGCTAACGGCAATGGAGAGCAGACATTAAGTCGGGATATTTACAGGCTTGGACACCGTTTTGACTTTTTCCGAATGATGTCATGTTACTTCACCACTGTTGGCTTTTATTTCAGCACCCTGATTACTGTTCTCACTGTCTACATCTTCCTTTATGGACGTCTCTATCTTGTTTTGAGTGGGCTCGAACAAGGTTTAAGTACGCAGAAAGGCATCCGAGACAATACACCTTTGCAAATAGCTCTGGCTTCACAGTCTTTTGTCCAGATTGGTTTCCTGATGGCTTTACCTATGCTTATGGAAATTGGATTAGAGAGGGGTTTTAGGACTGCCTTGAGTGAATTTGTGCTGATGCAGCTTCAGTTGGCGCCAGTGTTCTTTACGTTCTCTCTTGGGACAAAGACTCACTACTATGGGAGGACGTTACTTCATGGCGGTGCCAAGTACAGGTCCACAGGAAGAGGATTTGTCGTCTTTCATGCCAAATTTGCTGATAATTACAGACTGTATTCCCGGAGCCATTTTGTGAAGGGACTTGAAATGATGCTGCTACTCGTTGTGTATCAAATATTTGGTAGTGCTTACAGAGGCGTCCTTGCATATCTTTTGATCACCATATCTATGTGGTTCATGGTCGGGACCTGGCTTTTTGCTCCCTTCCTCTTCAATCCTTCTGGTTTTGAATGGCAGAAGATTGTTGATGATTGGACTGATTGGAATAAATGGATAAACAACATCGGGGGTATTGGTGTTCCGGCAGAAAAAAGTTGGGAATCGTGGTGGGAGGAAGAGCAAGAACATCTCCGGTATTCCGGAAAACGTGGTATAGTTGTTGAGATTTTGCTAGCACTGCGGTTCTTTATCTATCAATATGGGCTTGTTTATCATCTCACTATAACAGAGAAGACTAAAAACTTTCTGGTTTATGGAGTATCATGGCTGGTGATTTTCTTGATATTGTTTGTGATGAAGACTGTTTCGGTTGGAAGGCGGAGATTCAGCGCGAGTTTTCAGCTGATGTTCCGGTTGATAAAGGGGCTGATATTCATGACGTTTATCGCAATTATTGTGATATTGATCACACTGGCTCACATGACGATACAAGACATAATTGTGTGTATCCTTGCCTTTATGCCCACAGGTTGGGGGATGCTCTTGATTGCGCAAGCGTGTAAGCCGGTGGTTCATAGAGCAGGATTCTGGGGATCAGTGAGGACCCTAGCTCGTGGATACGAGATAGTAATGGGACTGTTGCTGTTCACGCCAGTGGCGTTCTTGGCATGGTTTCCATTTGTGTCAGAGTTCCAAACGCGTATGCTCTTTAATCAAGCTTTCAGTAGAGGTCTTCAGATCTCTCGTATCCTTGGAGGTCATCGTAAGGATCGCTCTTCTCGAAACAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1955

Amino Acids

226.18

Weight (kDa)

9.12

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 45 - 172 1.6e-17 Vta1 like
FKS1_dom1 PF14288 322 - 434 2.6e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 859 - 1050 1.4e-64 Callose synthase 1 domain
Glucan_synthase PF02364 1057 - 1148 7.9e-30 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1148 - 1775 1.5e-199 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1817, 2504
AatII GACGTC 1 cut(s) 4624
Acc16I TGCGCA 2 cut(s) 3741, 5625
Acc36I ACCTGC 1 cut(s) 3955
Acc65I GGTACC 1 cut(s) 2419
AccB1I GGYRCC 6 cut(s) 2419, 3071, 3241, 3871, 4828, 4898
AccB7I CCANNNNNTGG 3 cut(s) 1130, 2010, 5604
AccBSI CCGCTC 1 cut(s) 3406
AccI GTMKAC 1 cut(s) 4602
AccII CGCG 3 cut(s) 1856, 5465, 5773
AccIII TCCGGA 1 cut(s) 5273
AclI AACGTT 1 cut(s) 2390
AcoI YGGCCR 3 cut(s) 674, 1298, 4258
AcsI RAATTY 8 cut(s) 2110, 2729, 3214, 3610, 4072, 4085, 4802, 4946
AcuI CTGAAG 5 cut(s) 1432, 3315, 3530, 4805, 5792
AcyI GRCGYC 3 cut(s) 4621, 4829, 5052
AdeI CACNNNGTG 2 cut(s) 3782, 4373
AfaI GTAC 5 cut(s) 1344, 2421, 3524, 4663, 4907
AflII CTTAAG 2 cut(s) 3418, 3434
AflIII ACRYGT 2 cut(s) 1660, 5771
AhdI GACNNNNNGTC 1 cut(s) 4491
AhlI ACTAGT 1 cut(s) 4196
AjnI CCWGG 8 cut(s) 1491, 1511, 1703, 2320, 3321, 3915, 3940, 5102
AjuI GAANNNNNNNTTGG 2 cut(s) 624, 656
AleI CACNNNNGTG 2 cut(s) 2461, 5726
AloI GAACNNNNNNTCC 4 cut(s) 240, 272, 1596, 1628
Alw21I GWGCWC 2 cut(s) 2652, 2839
Alw26I GTCTC 7 cut(s) 42, 1040, 1944, 2949, 4187, 4628, 4674
Aor13HI TCCGGA 1 cut(s) 5273
ApaI GGGCCC 1 cut(s) 2179
ApoI RAATTY 8 cut(s) 2110, 2729, 3214, 3610, 4072, 4085, 4802, 4946
ArsI GACNNNNNNTTYG 2 cut(s) 907, 939
Asp700I GAANNNNTTC 4 cut(s) 111, 875, 1185, 1688
Asp718I GGTACC 1 cut(s) 2419
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 6 cut(s) 963, 1982, 3742, 4831, 5465, 5626
AsuC2I CCSGG 2 cut(s) 512, 4975
AsuNHI GCTAGC 2 cut(s) 2591, 5305
AvaII GGWCC 9 cut(s) 20, 1902, 3301, 3319, 3913, 3944, 4911, 5100, 5676
AvrII CCTAGG 1 cut(s) 179
AxyI CCTNAGG 1 cut(s) 3067
BaeGI GKGCMC 3 cut(s) 427, 2179, 3652
BalI TGGCCA 3 cut(s) 676, 1300, 4260
BamHI GGATCC 1 cut(s) 232
BanI GGYRCC 6 cut(s) 2419, 3071, 3241, 3871, 4828, 4898
BanII GRGCYC 3 cut(s) 2179, 2652, 4647
BauI CACGAG 4 cut(s) 1973, 2136, 3735, 5685
BbsI GAAGAC 6 cut(s) 659, 711, 1438, 5372, 5438, 5798
Bbv12I GWGCWC 2 cut(s) 2652, 2839
BbvCI CCTCAGC 1 cut(s) 45
BceAI ACGGC 2 cut(s) 19, 4486
BcgI CGANNNNNNTGC 2 cut(s) 1127, 1161
BciT130I CCWGG 8 cut(s) 1493, 1513, 1705, 2322, 3323, 3917, 3942, 5104
BciVI GTATCC 4 cut(s) 3722, 5591, 5684, 5831
BclI TGATCA 8 cut(s) 25, 823, 844, 2854, 2871, 3886, 5070, 5538
BcnI CCSGG 2 cut(s) 512, 4975
BcoDI GTCTC 7 cut(s) 42, 1040, 1944, 2949, 4187, 4628, 4674
BcuI ACTAGT 1 cut(s) 4196
BfmI CTRYAG 5 cut(s) 281, 1735, 1909, 3600, 3925
BfoI RGCGCY 1 cut(s) 4832
BfrI CTTAAG 2 cut(s) 3418, 3434
BfuAI ACCTGC 1 cut(s) 3955
BfuI GTATCC 4 cut(s) 3722, 5591, 5684, 5831
BglI GCCNNNNNGGC 1 cut(s) 2174
BglII AGATCT 3 cut(s) 2497, 4440, 5811
BlnI CCTAGG 1 cut(s) 179
Bme18I GGWCC 9 cut(s) 20, 1902, 3301, 3319, 3913, 3944, 4911, 5100, 5676
BmeRI GACNNNNNGTC 1 cut(s) 4491
BmrI ACTGGG 2 cut(s) 1116, 2297
BmtI GCTAGC 2 cut(s) 2595, 5309
BmuI ACTGGG 2 cut(s) 1116, 2297
BpiI GAAGAC 6 cut(s) 659, 711, 1438, 5372, 5438, 5798
BpmI CTGGAG 4 cut(s) 226, 1475, 1737, 2343
Bpu10I CCTNAGC 2 cut(s) 45, 816
BpuEI CTTGAG 7 cut(s) 513, 1036, 1174, 1916, 2636, 3422, 4816
BpuMI CCSGG 2 cut(s) 512, 4975
Bsa29I ATCGAT 1 cut(s) 4026
BsaBI GATNNNNATC 5 cut(s) 2559, 2768, 2907, 4032, 5537
BsaHI GRCGYC 3 cut(s) 4621, 4829, 5052
BsaI GGTCTC 1 cut(s) 1944
BsaWI WCCGGW 4 cut(s) 3725, 5265, 5273, 5484
BsaXI ACNNNNNCTCC 4 cut(s) 1536, 1566, 4043, 4073
Bse118I RCCGGY 1 cut(s) 5638
Bse1I ACTGG 9 cut(s) 243, 1111, 2292, 3095, 3812, 4156, 4832, 5550, 5726
Bse21I CCTNAGG 1 cut(s) 3067
Bse3DI GCAATG 1 cut(s) 4480
Bse8I GATNNNNATC 5 cut(s) 2559, 2768, 2907, 4032, 5537
BseAI TCCGGA 1 cut(s) 5273
BseBI CCWGG 8 cut(s) 1493, 1513, 1705, 2322, 3323, 3917, 3942, 5104
BseCI ATCGAT 1 cut(s) 4026
BseJI GATNNNNATC 5 cut(s) 2559, 2768, 2907, 4032, 5537
BseMI GCAATG 1 cut(s) 4480
BseNI ACTGG 9 cut(s) 243, 1111, 2292, 3095, 3812, 4156, 4832, 5550, 5726
BseRI GAGGAG 2 cut(s) 30, 3470
BseSI GKGCMC 3 cut(s) 427, 2179, 3652
BseYI CCCAGC 2 cut(s) 426, 1085
BsgI GTGCAG 1 cut(s) 258
Bsh1236I CGCG 3 cut(s) 1856, 5465, 5773
Bsh1285I CGRYCG 1 cut(s) 1462
BshNI GGYRCC 6 cut(s) 2419, 3071, 3241, 3871, 4828, 4898
BshVI ATCGAT 1 cut(s) 4026
BsiEI CGRYCG 1 cut(s) 1462
BsiHKAI GWGCWC 2 cut(s) 2652, 2839
BsiSI CCGG 8 cut(s) 511, 3726, 4975, 5216, 5266, 5274, 5485, 5639
BslFI GGGAC 7 cut(s) 548, 3155, 3332, 4870, 5007, 5113, 5721
BsmAI GTCTC 7 cut(s) 42, 1040, 1944, 2949, 4187, 4628, 4674
BsmBI CGTCTC 2 cut(s) 42, 4628
BsmFI GGGAC 7 cut(s) 548, 3155, 3332, 4870, 5007, 5113, 5721
BsmI GAATGC 7 cut(s) 1148, 1974, 2039, 2258, 2298, 2705, 3551
Bso31I GGTCTC 1 cut(s) 1944
Bsp120I GGGCCC 1 cut(s) 2175
Bsp1286I GDGCHC 6 cut(s) 427, 2179, 2652, 2839, 3652, 4647
Bsp13I TCCGGA 1 cut(s) 5273
BspDI ATCGAT 1 cut(s) 4026
BspEI TCCGGA 1 cut(s) 5273
BspFNI CGCG 3 cut(s) 1856, 5465, 5773
BspHI TCATGA 2 cut(s) 4393, 5508
BspMI ACCTGC 1 cut(s) 3955
BspOI GCTAGC 2 cut(s) 2595, 5309
BspQI GCTCTTC 3 cut(s) 2844, 5244, 5854
BspT107I GGYRCC 6 cut(s) 2419, 3071, 3241, 3871, 4828, 4898
BspTI CTTAAG 2 cut(s) 3418, 3434
BspTNI GGTCTC 1 cut(s) 1944
BsrBI CCGCTC 1 cut(s) 3406
BsrDI GCAATG 1 cut(s) 4480
BsrFI RCCGGY 1 cut(s) 5638
BsrI ACTGG 9 cut(s) 243, 1111, 2292, 3095, 3812, 4156, 4832, 5550, 5726
BssAI RCCGGY 1 cut(s) 5638
BssNI GRCGYC 3 cut(s) 4621, 4829, 5052
BssSI CACGAG 4 cut(s) 1973, 2136, 3735, 5685
BssT1I CCWWGG 7 cut(s) 179, 671, 907, 2269, 3537, 4320, 5824
Bst2BI CACGAG 4 cut(s) 1973, 2136, 3735, 5685
Bst2UI CCWGG 8 cut(s) 1493, 1513, 1705, 2322, 3323, 3917, 3942, 5104
BstACI GRCGYC 3 cut(s) 4621, 4829, 5052
BstAFI CTTAAG 2 cut(s) 3418, 3434
BstAPI GCANNNNNTGC 2 cut(s) 952, 2453
BstC8I GCNNGC 9 cut(s) 1083, 1125, 1308, 1376, 1854, 2037, 2593, 5307, 5628
BstDSI CCRYGG 1 cut(s) 4003
BstENI CCTNNNNNAGG 1 cut(s) 27
BstFNI CGCG 3 cut(s) 1856, 5465, 5773
BstH2I RGCGCY 1 cut(s) 4832
BstHHI GCGC 6 cut(s) 963, 1982, 3742, 4831, 5465, 5626
BstMAI GTCTC 7 cut(s) 42, 1040, 1944, 2949, 4187, 4628, 4674
BstMCI CGRYCG 1 cut(s) 1462
BstNI CCWGG 8 cut(s) 1493, 1513, 1705, 2322, 3323, 3917, 3942, 5104
BstNSI RCATGY 7 cut(s) 712, 1445, 1664, 1852, 2938, 3235, 3262
BstSFI CTRYAG 5 cut(s) 281, 1735, 1909, 3600, 3925
BstSLI GKGCMC 3 cut(s) 427, 2179, 3652
BstUI CGCG 3 cut(s) 1856, 5465, 5773
BstV2I GAAGAC 6 cut(s) 659, 711, 1438, 5372, 5438, 5798
BstX2I RGATCY 9 cut(s) 232, 627, 1747, 2497, 2521, 2560, 3198, 4440, 5811
BstXI CCANNNNNNTGG 2 cut(s) 2188, 3387
BstYI RGATCY 9 cut(s) 232, 627, 1747, 2497, 2521, 2560, 3198, 4440, 5811
Bsu15I ATCGAT 1 cut(s) 4026
Bsu36I CCTNAGG 1 cut(s) 3067
BsuI GTATCC 4 cut(s) 3722, 5591, 5684, 5831
BsuTUI ATCGAT 1 cut(s) 4026
BtgI CCRYGG 1 cut(s) 4003
BtsI GCAGTG 2 cut(s) 2315, 5309
BveI ACCTGC 1 cut(s) 3955
Cac8I GCNNGC 9 cut(s) 1083, 1125, 1308, 1376, 1854, 2037, 2593, 5307, 5628
CciI TCATGA 2 cut(s) 4393, 5508
CfoI GCGC 6 cut(s) 963, 1982, 3742, 4831, 5465, 5626
Cfr10I RCCGGY 1 cut(s) 5638
ClaI ATCGAT 1 cut(s) 4026
CseI GACGC 2 cut(s) 275, 5041
CsiI ACCWGGT 1 cut(s) 3915
Csp6I GTAC 5 cut(s) 1343, 2420, 3523, 4662, 4906
CspCI CAANNNNNGTGG 2 cut(s) 2221, 2256
CviQI GTAC 5 cut(s) 1343, 2420, 3523, 4662, 4906
DinI GGCGCC 1 cut(s) 4830
DraIII CACNNNGTG 2 cut(s) 3782, 4373
DriI GACNNNNNGTC 1 cut(s) 4491
EaeI YGGCCR 3 cut(s) 674, 1298, 4258
Eam1105I GACNNNNNGTC 1 cut(s) 4491
EciI GGCGGA 5 cut(s) 70, 1536, 2252, 3211, 5467
Ecl136II GAGCTC 1 cut(s) 2650
Eco130I CCWWGG 7 cut(s) 179, 671, 907, 2269, 3537, 4320, 5824
Eco147I AGGCCT 1 cut(s) 2065
Eco24I GRGCYC 3 cut(s) 2179, 2652, 4647
Eco31I GGTCTC 1 cut(s) 1944
Eco32I GATATC 1 cut(s) 620
Eco47I GGWCC 9 cut(s) 20, 1902, 3301, 3319, 3913, 3944, 4911, 5100, 5676
Eco53kI GAGCTC 1 cut(s) 2650
Eco57I CTGAAG 5 cut(s) 1432, 3315, 3530, 4805, 5792
Eco81I CCTNAGG 1 cut(s) 3067
EcoICRI GAGCTC 1 cut(s) 2650
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoO109I RGGNCCY 6 cut(s) 20, 2176, 3301, 3319, 5100, 5676
EcoRII CCWGG 8 cut(s) 1491, 1511, 1703, 2320, 3321, 3915, 3940, 5102
EcoRV GATATC 1 cut(s) 620
EcoT14I CCWWGG 7 cut(s) 179, 671, 907, 2269, 3537, 4320, 5824
EcoT22I ATGCAT 3 cut(s) 211, 1150, 3573
EcoT38I GRGCYC 3 cut(s) 2179, 2652, 4647
EgeI GGCGCC 1 cut(s) 4830
EheI GGCGCC 1 cut(s) 4830
ErhI CCWWGG 7 cut(s) 179, 671, 907, 2269, 3537, 4320, 5824
Esp3I CGTCTC 2 cut(s) 42, 4628
FaqI GGGAC 7 cut(s) 548, 3155, 3332, 4870, 5007, 5113, 5721
FauI CCCGC 3 cut(s) 1849, 3309, 3399
FauNDI CATATG 2 cut(s) 1056, 3685
FbaI TGATCA 8 cut(s) 25, 823, 844, 2854, 2871, 3886, 5070, 5538
FblI GTMKAC 1 cut(s) 4602
FriOI GRGCYC 3 cut(s) 2179, 2652, 4647
FspI TGCGCA 2 cut(s) 3741, 5625
GlaI GCGC 6 cut(s) 962, 1981, 3741, 4830, 5464, 5625
GsaI CCCAGC 2 cut(s) 430, 1089
GsuI CTGGAG 4 cut(s) 226, 1475, 1737, 2343
HaeII RGCGCY 1 cut(s) 4832
HapII CCGG 8 cut(s) 511, 3726, 4975, 5216, 5266, 5274, 5485, 5639
HgaI GACGC 2 cut(s) 275, 5041
HhaI GCGC 6 cut(s) 963, 1982, 3742, 4831, 5465, 5626
Hin1I GRCGYC 3 cut(s) 4621, 4829, 5052
Hin6I GCGC 6 cut(s) 961, 1980, 3740, 4829, 5463, 5624
HinP1I GCGC 6 cut(s) 961, 1980, 3740, 4829, 5463, 5624
HincII GTYRAC 5 cut(s) 508, 2788, 3144, 3701, 3882
HindII GTYRAC 5 cut(s) 508, 2788, 3144, 3701, 3882
HindIII AAGCTT 5 cut(s) 355, 390, 872, 3105, 5790
HpaII CCGG 8 cut(s) 511, 3726, 4975, 5216, 5266, 5274, 5485, 5639
Hpy99I CGWCG 1 cut(s) 1890
HpyCH4IV ACGT 8 cut(s) 1956, 2390, 2913, 4621, 4844, 4883, 5281, 5513
HpySE526I ACGT 8 cut(s) 1956, 2390, 2913, 4621, 4844, 4883, 5281, 5513
Hsp92I GRCGYC 3 cut(s) 4621, 4829, 5052
HspAI GCGC 6 cut(s) 961, 1980, 3740, 4829, 5463, 5624
KasI GGCGCC 1 cut(s) 4828
Kpn2I TCCGGA 1 cut(s) 5273
KpnI GGTACC 1 cut(s) 2423
Ksp22I TGATCA 8 cut(s) 25, 823, 844, 2854, 2871, 3886, 5070, 5538
LguI GCTCTTC 3 cut(s) 2844, 5244, 5854
LmnI GCTCC 7 cut(s) 769, 1470, 1544, 2324, 3541, 4977, 5119
MabI ACCWGGT 1 cut(s) 3915
MaeII ACGT 8 cut(s) 1956, 2390, 2913, 4621, 4844, 4883, 5281, 5513
MbiI CCGCTC 1 cut(s) 3406
MflI RGATCY 9 cut(s) 232, 627, 1747, 2497, 2521, 2560, 3198, 4440, 5811
MhlI GDGCHC 6 cut(s) 427, 2179, 2652, 2839, 3652, 4647
MlsI TGGCCA 3 cut(s) 676, 1300, 4260
MluI ACGCGT 1 cut(s) 5771
MluNI TGGCCA 3 cut(s) 676, 1300, 4260
Mly113I GGCGCC 1 cut(s) 4829
MlyI GAGTC 7 cut(s) 101, 1162, 1944, 3164, 3274, 3449, 4858
MmeI TCCRAC 3 cut(s) 332, 1929, 5425
Mox20I TGGCCA 3 cut(s) 676, 1300, 4260
Mph1103I ATGCAT 3 cut(s) 211, 1150, 3573
MroI TCCGGA 1 cut(s) 5273
MroXI GAANNNNTTC 4 cut(s) 111, 875, 1185, 1688
MscI TGGCCA 3 cut(s) 676, 1300, 4260
Msp20I TGGCCA 3 cut(s) 676, 1300, 4260
MspA1I CMGCKG 6 cut(s) 52, 430, 1586, 1740, 3055, 5476
MspCI CTTAAG 2 cut(s) 3418, 3434
MspI CCGG 8 cut(s) 511, 3726, 4975, 5216, 5266, 5274, 5485, 5639
Mva1269I GAATGC 7 cut(s) 1148, 1974, 2039, 2258, 2298, 2705, 3551
MvaI CCWGG 8 cut(s) 1493, 1513, 1705, 2322, 3323, 3917, 3942, 5104
MvnI CGCG 3 cut(s) 1856, 5465, 5773
NarI GGCGCC 1 cut(s) 4829
NciI CCSGG 2 cut(s) 512, 4975
NdeI CATATG 2 cut(s) 1056, 3685
NheI GCTAGC 2 cut(s) 2591, 5305
NmuCI GTSAC 5 cut(s) 683, 1165, 1839, 2104, 2669
NsbI TGCGCA 2 cut(s) 3741, 5625
NsiI ATGCAT 3 cut(s) 211, 1150, 3573
NspI RCATGY 7 cut(s) 712, 1445, 1664, 1852, 2938, 3235, 3262
OliI CACNNNNGTG 2 cut(s) 2461, 5726
PagI TCATGA 2 cut(s) 4393, 5508
PceI AGGCCT 1 cut(s) 2065
PciI ACATGT 1 cut(s) 1660
PciSI GCTCTTC 3 cut(s) 2844, 5244, 5854
PcsI WCGNNNNNNNCGW 2 cut(s) 5729, 5851
PctI GAATGC 7 cut(s) 1148, 1974, 2039, 2258, 2298, 2705, 3551
PdmI GAANNNNTTC 4 cut(s) 111, 875, 1185, 1688
PflFI GACNNNGTC 1 cut(s) 493
PflMI CCANNNNNTGG 3 cut(s) 1130, 2010, 5604
PfoI TCCNGGA 2 cut(s) 3940, 4973
Ple19I CGATCG 1 cut(s) 1462
PleI GAGTC 7 cut(s) 100, 1162, 1944, 3163, 3274, 3448, 4858
PluTI GGCGCC 1 cut(s) 4832
PpsI GAGTC 7 cut(s) 100, 1162, 1944, 3163, 3274, 3448, 4858
PpuMI RGGWCCY 5 cut(s) 20, 3301, 3319, 5100, 5676
PscI ACATGT 1 cut(s) 1660
PsiI TTATAA 2 cut(s) 1817, 2504
Psp124BI GAGCTC 1 cut(s) 2652
Psp1406I AACGTT 1 cut(s) 2390
Psp5II RGGWCCY 5 cut(s) 20, 3301, 3319, 5100, 5676
Psp6I CCWGG 8 cut(s) 1491, 1511, 1703, 2320, 3321, 3915, 3940, 5102
PspFI CCCAGC 2 cut(s) 426, 1085
PspGI CCWGG 8 cut(s) 1491, 1511, 1703, 2320, 3321, 3915, 3940, 5102
PspOMI GGGCCC 1 cut(s) 2175
PspPPI RGGWCCY 5 cut(s) 20, 3301, 3319, 5100, 5676
PsuI RGATCY 9 cut(s) 232, 627, 1747, 2497, 2521, 2560, 3198, 4440, 5811
PsyI GACNNNGTC 1 cut(s) 493
PvuI CGATCG 1 cut(s) 1462
PvuII CAGCTG 5 cut(s) 430, 1586, 1740, 3055, 5476
RsaI GTAC 5 cut(s) 1344, 2421, 3524, 4663, 4907
RsaNI GTAC 5 cut(s) 1343, 2420, 3523, 4662, 4906
SacI GAGCTC 1 cut(s) 2652
SapI GCTCTTC 3 cut(s) 2844, 5244, 5854
SchI GAGTC 7 cut(s) 101, 1162, 1944, 3164, 3274, 3449, 4858
SduI GDGCHC 6 cut(s) 427, 2179, 2652, 2839, 3652, 4647
SexAI ACCWGGT 1 cut(s) 3915
SfcI CTRYAG 5 cut(s) 281, 1735, 1909, 3600, 3925
SfoI GGCGCC 1 cut(s) 4830
SinI GGWCC 9 cut(s) 20, 1902, 3301, 3319, 3913, 3944, 4911, 5100, 5676
SmlI CTYRAG 9 cut(s) 528, 1015, 1153, 1895, 2651, 3401, 3418, 3434, 4795
SmoI CTYRAG 9 cut(s) 528, 1015, 1153, 1895, 2651, 3401, 3418, 3434, 4795
SpeI ACTAGT 1 cut(s) 4196
SseBI AGGCCT 1 cut(s) 2065
SspDI GGCGCC 1 cut(s) 4828
SspI AATATT 3 cut(s) 2152, 2879, 5031
SstI GAGCTC 1 cut(s) 2652
StuI AGGCCT 1 cut(s) 2065
StyI CCWWGG 7 cut(s) 179, 671, 907, 2269, 3537, 4320, 5824
TaiI ACGT 8 cut(s) 1959, 2393, 2916, 4624, 4847, 4886, 5284, 5516
TatI WGTACW 2 cut(s) 3522, 4905
TauI GCSGC 2 cut(s) 55, 2268
TseFI GTSAC 5 cut(s) 683, 1165, 1839, 2104, 2669
Tsp45I GTSAC 5 cut(s) 683, 1165, 1839, 2104, 2669
TspGWI ACGGA 4 cut(s) 2423, 2684, 3142, 3770
Tth111I GACNNNGTC 1 cut(s) 493
Van91I CCANNNNNTGG 3 cut(s) 1130, 2010, 5604
Vha464I CTTAAG 2 cut(s) 3418, 3434
VpaK11BI GGWCC 9 cut(s) 20, 1902, 3301, 3319, 3913, 3944, 4911, 5100, 5676
XagI CCTNNNNNAGG 1 cut(s) 27
XapI RAATTY 8 cut(s) 2110, 2729, 3214, 3610, 4072, 4085, 4802, 4946
XceI RCATGY 7 cut(s) 712, 1445, 1664, 1852, 2938, 3235, 3262
XcmI CCANNNNNNNNNTGG 1 cut(s) 1504
XmaJI CCTAGG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 4602
XmnI GAANNNNTTC 4 cut(s) 111, 875, 1185, 1688
ZraI GACGTC 1 cut(s) 4622
Zsp2I ATGCAT 3 cut(s) 211, 1150, 3573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.