Rorug05G0471300

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005959
Physical Location & Seq
Reverse (-)
64764989 .. 64767892
2904 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0471300.1

Sequence Viewer

Length: 393 bp
ATGAAAGAAGCGAAAGCCAACGGTCAGTACCCGCAGCCGCAGCAGCACCAGAACGGCGGTGCCCACTTGTCGCCGTTCAAATTCGCCAAGTTGTTCGATCCAGAAGCTTCCTGGGACAAGGATCAATTGGGAGATGTCTTGCATTGGATTCGACAGGGCATGGCCCTTGTGTGTGGGCTGCTATGGGGTGCGGTACCCTTGGTAGGAGGCATATGGTTCGTCCTTTTTCTCGTGTTATCCACTGGGATTATATATGGTTACTATGCGATGATACTGAAGGTTGATGAAGAAGAATTTGGCGGTCATGGAGCTCTCCTCCAAGAGGGACTTTTTGCATCTGTAACTCTTTTTCTGCTTGCATGGGTTCTAGTATACAGCTTGGGACACTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

130

Amino Acids

14.44

Weight (kDa)

5.61

Isoelectric Point (pI)

26.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC6 PF07019 46 - 125 4.1e-17 EMC6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 193
AccB1I GGYRCC 2 cut(s) 59, 193
AccI GTMKAC 1 cut(s) 372
AciI CCGC 5 cut(s) 32, 38, 57, 191, 300
AclWI GGATC 2 cut(s) 92, 129
AcsI RAATTY 2 cut(s) 80, 293
AcuI CTGAAG 1 cut(s) 296
AfaI GTAC 2 cut(s) 29, 195
AfiI CCNNNNNNNGG 2 cut(s) 203, 322
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 1 cut(s) 110
AjuI GAANNNNNNNTTGG 2 cut(s) 279, 311
AluBI AGCT 3 cut(s) 107, 311, 378
AluI AGCT 3 cut(s) 107, 311, 378
Alw21I GWGCWC 1 cut(s) 313
AlwI GGATC 2 cut(s) 92, 129
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 4 cut(s) 34, 40, 43, 178
ApoI RAATTY 2 cut(s) 80, 293
Asp718I GGTACC 1 cut(s) 193
AspS9I GGNCC 1 cut(s) 163
BaeGI GKGCMC 1 cut(s) 64
BanI GGYRCC 2 cut(s) 59, 193
BanII GRGCYC 1 cut(s) 313
BauI CACGAG 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 4 cut(s) 46, 52, 55, 165
BceAI ACGGC 2 cut(s) 58, 70
BcgI CGANNNNNNTGC 4 cut(s) 131, 165, 199, 233
BciT130I CCWGG 1 cut(s) 112
BfaI CTAG 1 cut(s) 368
BisI GCNGC 5 cut(s) 35, 38, 41, 44, 179
BlsI GCNGC 5 cut(s) 36, 39, 42, 45, 180
Bme1390I CCNGG 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 2 cut(s) 61, 195
BmrFI CCNGG 1 cut(s) 112
BmrI ACTGGG 1 cut(s) 252
BmsI GCATC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 252
BplI GAGNNNNNCTC 2 cut(s) 300, 332
BsaJI CCNNGG 2 cut(s) 111, 198
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 322
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 111, 198
BseLI CCNNNNNNNGG 2 cut(s) 203, 322
BseNI ACTGG 1 cut(s) 247
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 64
BseXI GCAGC 4 cut(s) 46, 52, 55, 165
BshFI GGCC 1 cut(s) 164
BshNI GGYRCC 2 cut(s) 59, 193
BsiHKAI GWGCWC 1 cut(s) 313
BslFI GGGAC 2 cut(s) 128, 339
BslI CCNNNNNNNGG 2 cut(s) 203, 322
BsmFI GGGAC 2 cut(s) 128, 339
BsnI GGCC 1 cut(s) 164
Bsp1286I GDGCHC 2 cut(s) 64, 313
Bsp143I GATC 2 cut(s) 97, 121
BspACI CCGC 5 cut(s) 32, 38, 57, 191, 300
BspANI GGCC 1 cut(s) 164
BspLI GGNNCC 2 cut(s) 61, 195
BspPI GGATC 2 cut(s) 92, 129
BspT107I GGYRCC 2 cut(s) 59, 193
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 2 cut(s) 111, 198
BssMI GATC 2 cut(s) 97, 121
BssNAI GTATAC 1 cut(s) 373
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 1 cut(s) 198
Bst1107I GTATAC 1 cut(s) 373
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 320
BstKTI GATC 2 cut(s) 100, 124
BstMBI GATC 2 cut(s) 97, 121
BstMWI GCNNNNNNNGC 2 cut(s) 40, 43
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 110
BstSLI GKGCMC 1 cut(s) 64
BstV1I GCAGC 4 cut(s) 46, 52, 55, 165
BstZ17I GTATAC 1 cut(s) 373
BsuRI GGCC 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 281
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 163
Csp6I GTAC 2 cut(s) 28, 194
CviAII CATG 3 cut(s) 160, 305, 360
CviJI RGCY 7 cut(s) 17, 37, 107, 164, 178, 311, 378
CviKI_1 RGCY 7 cut(s) 17, 37, 107, 164, 178, 311, 378
CviQI GTAC 2 cut(s) 28, 194
DpnI GATC 2 cut(s) 99, 123
DpnII GATC 2 cut(s) 97, 121
Ecl136II GAGCTC 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 198
Eco24I GRGCYC 1 cut(s) 313
Eco53kI GAGCTC 1 cut(s) 311
Eco57I CTGAAG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 311
EcoNI CCTNNNNNAGG 1 cut(s) 320
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 198
EcoT38I GRGCYC 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 3 cut(s) 163, 308, 363
FalI AAGNNNNNCTT 2 cut(s) 312, 344
FaqI GGGAC 2 cut(s) 128, 339
FatI CATG 3 cut(s) 159, 304, 359
FauI CCCGC 1 cut(s) 39
FauNDI CATATG 1 cut(s) 212
FblI GTMKAC 1 cut(s) 372
Fnu4HI GCNGC 5 cut(s) 35, 38, 41, 44, 179
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 5 cut(s) 35, 38, 41, 44, 179
FspBI CTAG 1 cut(s) 368
GluI GCNGC 5 cut(s) 35, 38, 41, 44, 179
HaeIII GGCC 1 cut(s) 164
Hin1II CATG 3 cut(s) 163, 308, 363
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 1 cut(s) 148
Hpy166II GTNNAC 1 cut(s) 373
Hpy188I TCNGA 1 cut(s) 392
Hpy188III TCNNGA 1 cut(s) 101
Hpy8I GTNNAC 1 cut(s) 373
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 1 cut(s) 23
HpyCH4V TGCA 3 cut(s) 142, 335, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 40, 43
Hsp92II CATG 3 cut(s) 163, 308, 363
KpnI GGTACC 1 cut(s) 197
Kzo9I GATC 2 cut(s) 97, 121
LmnI GCTCC 1 cut(s) 308
LpnPI CCDG 6 cut(s) 62, 97, 114, 124, 140, 228
Lsp1109I GCAGC 4 cut(s) 46, 52, 55, 165
LweI GCATC 1 cut(s) 344
MaeI CTAG 1 cut(s) 368
MaeIII GTNAC 2 cut(s) 257, 340
MalI GATC 2 cut(s) 99, 123
MboI GATC 2 cut(s) 97, 121
MboII GAAGA 2 cut(s) 299, 302
MfeI CAATTG 1 cut(s) 125
MhlI GDGCHC 2 cut(s) 64, 313
MluCI AATT 3 cut(s) 80, 125, 293
MnlI CCTC 3 cut(s) 200, 316, 326
MspR9I CCNGG 1 cut(s) 112
MunI CAATTG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 112
MwoI GCNNNNNNNGC 2 cut(s) 40, 43
NdeI CATATG 1 cut(s) 212
NdeII GATC 2 cut(s) 97, 121
NlaIII CATG 3 cut(s) 163, 308, 363
NlaIV GGNNCC 2 cut(s) 61, 195
PfeI GAWTC 1 cut(s) 148
PkrI GCNGC 5 cut(s) 36, 39, 42, 45, 180
Psp124BI GAGCTC 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 2 cut(s) 61, 195
PspPI GGNCC 1 cut(s) 163
RsaI GTAC 2 cut(s) 29, 195
RsaNI GTAC 2 cut(s) 28, 194
SacI GAGCTC 1 cut(s) 313
SatI GCNGC 5 cut(s) 35, 38, 41, 44, 179
Sau3AI GATC 2 cut(s) 97, 121
Sau96I GGNCC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 2 cut(s) 64, 313
SetI ASST 4 cut(s) 109, 282, 313, 380
SfaNI GCATC 1 cut(s) 344
Sse9I AATT 3 cut(s) 80, 125, 293
SsiI CCGC 5 cut(s) 32, 38, 57, 191, 300
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 23
TaqI TCGA 2 cut(s) 96, 151
TasI AATT 3 cut(s) 80, 125, 293
TauI GCSGC 1 cut(s) 40
TfiI GAWTC 1 cut(s) 148
TscAI CASTG 1 cut(s) 247
TseI GCWGC 4 cut(s) 34, 40, 43, 178
TspDTI ATGAA 2 cut(s) 17, 300
TspRI CASTG 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 320
XapI RAATTY 2 cut(s) 80, 293
XcmI CCANNNNNNNNNTGG 1 cut(s) 108
XmiI GTMKAC 1 cut(s) 372
XspI CTAG 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.