RchiOBHm_Chr5g0080661

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005959
Physical Location & Seq
Forward (+)
86636030 .. 86651559
15530 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35495

Sequence Viewer

Length: 5883 bp
ATGTCGTCGTCGAGAGCGGGGCCGGATCAGGGGCCGCCGCCGCAGCGGCGTATCCAGCGGACGCAGACAGCTGGAAACCTCGGGGAGGCGGCTTTCGACAGTGAGGTGGTGCCGTCATCGCTTGTCGAAATCGCTCCCATTCTTCGTGTGGCCAACGAGGTCGAATCGCACAATCCCAGAGTTGCTTATCTATGTCGATTTTATGCCTTTGAGAAAGCTCATAGGTTGGATCCCACTTCTAGTGGACGTGGTGTTCGTCAGTTCAAAACTGCTCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACTCTTATGGGAAGGGTAAAAAAAAGTGATGCACGTGAAATGCAGAGCTTCTATCAACACTATTACAAAAAATATATTCAAGCTTTGCAAAATGCGGCCGATAAAGCTGACCGCGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTATTTGAGGTTTTAAAGGCTGTTAATATGACGCAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGGTAAAGTTGCAGAAAAGACAGAGCTATTGGTTCCTTACAATATCCTTCCACTTGATCCTGATAGTGCAAAACAAGCAATTATGACATACCCAGAGATTCAAGCTGCTGTTTTTGCTCTTCGTAACACCAGGGGTCTTCCTTGGCCTAAGGAATACAAAAAGAAAAAAGATGAGGATGTTCTTGATTGGCTGCAGTCAATGTTTGGGTTTCAGAAGGACAATGTGGCGAATCAACGGGAGCACCTGATATTGTTACTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAGCCCAAGTTGGATGACCGTGCCCTGACAGAAGTGATGAAGAAGCTTTTCAAGAATTACAAAAAATGGTGCAAGTTCTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACCATACAGCAAGAGGTGCAACAGCGCAAGCTGCTTTACATGGGTCTATATCTTTTGATATGGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGTTACATTTATCATCATATGGCATTTGAATTGTATGGTATGTTAGCTGGGAATGTGAGTCCAATGACAGGAGAAAATGTGAAGCCAGCCTATGGAGGTGAAGAAGAGGCTTTCTTGAAAAAAGTTGTGACTCCTATCTATAACGTGATTGCCAGGGAAGCTGATTGGAGCAAACGAGGGAAATCAAAGCATTCCCAGTGGAGGAACTATGATGATATAAATGAATACTTTTGGTCAGTGGATTGTTTCCGGTTGGGTTGGCCGATGCGTGCAGATGCTGATTTCTTTTGCTTGCCTAGTGAACGACTTAGTTTTGATAAAAATAGTGAGGATAGCAAACCAGCTAGTGGAGATCGATGGGTGGGGAAAGTTAACTTTGTTGAGATACGGTCATTCTGGCATATCTTTAGAAGCTTTGACCGCATGTGGAGTTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAATGGCTCGGGCCAACCCACATCAATATTTTCTGCCGATGTATTCAAGAAAGCTTTGAGCGTCTTTATAACTGCTGCAATATTGAAGCTTGGGCAAGCTGTTCTAGATGTAATTCTTAGCTGGAAGTCTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATATATTGCTAAGGTCATTTCAGCTGCTGCATGGGTAATAATTCTACCGGTTACTTATGCGTATACTTGGGAAAACCCTCCTGGGTTTGCTCAGACCATTAAAGGTTGGTTTGGCAGTAATAAAAATGCGCCCTCCTTGTTCATCTTGGCTGTTGTTATCTACTTGTCACCGAATATGCTGGCTGGGGTGTTGTTTCTTTTCCCATTTATTCGCCGATTCCTTGAGAGATCAAACTATAGGATTGTGATGCTTATGATGTGGTGGTCACAGCCTCGTCTCTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCTCTTTTCAAGTACACGATGTTTTGGGTTCTTCTTATAGTGACAAAGTTGGCATTCAGTTACTACATAGAGATAAAGCCTTTAGTGGGTCCAACAAAAGCTATCATGAATGTACGTATAACGAATTTCCAGTGGCATGAGTTCTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTGGTCTATTTTATGGATACCCAGATTTGGTATGCAATATACTCGACAATATTCGGAGGTATTTATGGTGCATTCCGTCGCCTTGGAGAGATACGGACACTAGGAATGCTAAGATCTCGCTTTGAATCATTGCCTGGTGCTTTTAACGCCCGTTTAATTCCGGTGGATAAGAGTGAGCCAAAGAAGAAAGGACTGAAGGCTACATTGTCCCGCACCTTTGGTCATGTTGAAGGTAGCAAAGAGAAACAGGCTGCAAGGTTTGCGCAATTATGGAACAAAATAATAAGTAGTTTCAGAGAGGAGGATCTTATAAGCAATAGGGAAATGGACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGATTTGGACCTCATACAGTGGCCTCCATTTTTACTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGCTAACAAAAAGGATCACGGCTGATGAATACATGCATTGCGCTGTTCGTGAATGCTATGCTTCATTTAAGAACATAATTAAGTTTCTGGTTCAGGGGAGCCGTGAGAAAGAGGTTATAGATTACATATTTCTTGAGGTTGACAAGCACATAGGAGATGGTAACCTGATCCGTGAATTCAAGATGAGTGCTCTTCCTAGCCTCTATGACCACTTTGTTAGCCTTATCAATTTTTTGTTAAAGAATAGTCAGGAGGACAGGGATCAAGTTGTGATTCTATTCCAGGACATGCTGGAGGTAGTGACAAGAGATATAATGATGGAGGACCAGGACCATATAACCAGCTTGGTAGATTCAGTCCACGGAGGGTCGGGCCATGAGGGGATGATGCCTCTTGATCAACATCAACAGCATCAGTTGTTTGCATCTGCTGGAGCTATCAACTTTCCTCTTACACATGTAACAGAGGCTTGGAAGGAGAAGATTAACCGTCTTTATCTATTACTTACCACAAAGGAGTCTGCCATGGATGTGCCATCCAACTTAGAAGCTAGAAGGCGAATTTCTTTCTTCTCGAATTCATTGTTTATGGATATGCCTCCAGCACCAAAAGTTCGCAATATGCTTTCTTTCTCTGTTTTAACTCCTTACTACACCGAGGAGGTTCTCTTTTCCATTGAAGGTCTGGAAAAACCGAATGAAGATGGTGTTTCTATTCTCTTCTACTTGCAAAAGATTTTCCCAGATGAATGGAACAACTTTCTTCAGCGTGTGAATTGCACCAACGAAGATGAATTGAACAACTTTCTTAAAGGGTCTGATGAATTGGAAGAAGAACTTCGCCTATGGGCATCATATAGAGGCCAAACTTTGACTCGTACTGTAAGAGGGATGATGTACTACCGGAAAGCTTTGGAGCTTCAGGCTTTTCTGGATATGGCCAAAGATGACGATTTAATGGAAGGCTATAAGGCCATAGAGTTGAATTCAGAAGATCAATCAAAGGGGGGAAGGTCACTATGGGCGCAATGTCAAGCAGTAGCTGATATGAAATTCACATATGTGGTTTCATGCCAACTGTATGGGATTCAAAAGCGATCTGGTGATCATCATGCACAAGACATTCTGAGGCTCATGACAACATACCCGTCATTACGAGTGGCATATATTGATGAGGTTGAAGAACCTAGCAAAGATAGGGCTCAGAAGATAAACCAAAAGGCTTATTATTCTACTTTAGTAAAGGCTGCTATGCCAAAGTCAATTGATTCTTCAGAGCCAGTACAAAATCTTGACCAGGTTATTTATCGCATAAAGCTTCCGGGACCTGCTATCTTGGGAGAGGGAAAGCCAGAAAATCAAAACCATGCCATTATTTTCACACGTGGAGAAGGCTTACAAACAATTGACATGAACCAGGATAACTACATGGAAGAAGCTTTGAAAATGAGGAATTTGCTACAAGAATTTCTCAAACATGATGGCGTTAGGCACCCTACAATTCTTGGACTTAGGGAGCATATATTTACGGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAGAACAGTTTTGTTACTATTGGTCAAAGACTATTGGCCAACCCTCTCAGGGTTCGATTTCATTATGGCCATCCCGATGTGTTTGATAGGCTCTTTCACCTTAGTAGAGGGGGTGTCAGTAAGGCATCCAAGGTTATCAATTTGAGTGAAGACATTTTTGCAGGCTTTAATTCCACTCTCCGTGAAGGCAATGTAACTCATCACGAATACATACAAGTGGGGAAGGGGAGAGATGTCGGTCTTAACCAAATCTCCATGTTTGAGGCAAAAATTGCTAATGGCAACGGGGAGCAGACACTCAGTCGTGATATATACCGACTTGGACATCGTTTTGACTTTTTCCGTATGTTGTCATGCTATTTCACCACAATTGGTTTCTACTACAGTACTCTGATCACTGTCCTTACAGTGTATGTCTTCCTTTATGGTCGCCTCTACCTGGTTCTTAGTGGACTTGAAGAAGGTTTGAGCACTCAAGCAGCAATTCGAGATAATAAGCCTCTTCAAGTTGCTCTTGCTTCTCAATCATTTGTTCAAATAGGGTTTTTGATGGCTTTGCCTATGTTGATGGAAATTGGCTTGGAAAAGGGTTTCCGAACTGCATTAAGTGAATTCGTGTTGATGCAATTGCAATTGGCCCCAGTATTTTTCACATTCTCACTTGGGACAAAGACCCACTATTATGGACGGACATTACTGCATGGTGGTGCCAAATATAGATCGACAGGTCGTGGGTTTGTGGTGTTCCATGCCAAGTTTGCCGACAACTATAGGCTCTACTCTCGCAGCCACTTTGTTAAGGGTATTGAGCTCTTGATTTTACTTGTGGTGTACCAGATCTTTGGTCATACTTATAGAAGTGCTGTTGCCTACATTTTAATCACTGTATCTATGTGGTTTATGGTGGTTACCTGGCTTTTTGCTCCCTTTCTGTTCAATCCCTCTGGTTTTGAGTGGCAAAAGATAGTTGATGATTGGACTGATTGGAATAAGTGGATAAGCAACCGTGGAGGTATAGGTGTTCCACCTGAAAAAAGTTGGGAATCATGGTGGGAGGAGGAACAAGAACATCTTCAATATTCTGGAAAACGTGGTATTGTAGCTGAGATACTGCTATCCGTACGTTTCTTTATCTATCAGTATGGGCTCGTATATCACTTGAACATTGCAAAGAAAACCAAGAGTGTCCTGGTCTATGGTATCTCATGGCTGGTGATCGTCCTTATTTTGTTTGTCATGAAGACTGTATCTGTTGGTAGGAGAAAGTTCAGTGCTGAGTACCAACTTGTATTCCGGCTGATCAAGGGATTAATATTCATTACTTTTGTGTCCATTTTGGTCACTTTGATTGTGCTGCCTCACATGACCCTGCAGGACATCATTGTTTGCATTCTTGCTTTCATGCCAACTGGTTGGGGGATGCTTATGATTGCCCAAGCTTGCAAGCCTATTGTTCAAAAAGCTGGTTTATGGCCATCAGTTCGGACTCTTGCTCGTGGCTTTGAGATTGTTATGGGCTTGCTTCTGTTCACCCCAGTTGCATTCTTGGCCTGGTTTCCTTTTGTTTCAGAATTTCAAACTCGTATGCTCTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCTCGTATTCTTGGCGGGCAACGCAAGGATCGTTCATCCCGGAACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1960

Amino Acids

225.86

Weight (kDa)

9.16

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 44 - 172 1.4e-17 Vta1 like
FKS1_dom1 PF14288 321 - 433 9.9e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 856 - 1050 1.9e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1057 - 1854 7.9e-239 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1577, 2495
Acc16I TGCGCA 1 cut(s) 2448
Acc36I ACCTGC 1 cut(s) 3973
AccB1I GGYRCC 3 cut(s) 109, 4128, 4913
AccB7I CCANNNNNTGG 2 cut(s) 1127, 1382
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 1733
AccII CGCG 1 cut(s) 420
AcoI YGGCCR 8 cut(s) 150, 402, 1295, 2610, 3576, 4242, 4273, 5678
AcuI CTGAAG 4 cut(s) 2399, 3386, 3542, 3894
AcvI CACGTG 2 cut(s) 341, 4022
AfaI GTAC 9 cut(s) 1997, 2097, 3517, 3536, 3921, 4594, 5039, 5328, 5486
AflIII ACRYGT 2 cut(s) 3094, 4019
AgeI ACCGGT 1 cut(s) 1717
AhdI GACNNNNNGTC 1 cut(s) 4506
AjiI CACGTC 1 cut(s) 248
AleI CACNNNNGTG 1 cut(s) 3698
AloI GAACNNNNNNTCC 2 cut(s) 237, 269
Alw21I GWGCWC 6 cut(s) 771, 798, 1979, 2830, 4679, 5019
Alw26I GTCTC 1 cut(s) 1952
Alw44I GTGCAC 1 cut(s) 794
AlwNI CAGNNNCTG 3 cut(s) 1313, 1697, 3680
Ama87I CYCGRG 2 cut(s) 80, 1516
ApaLI GTGCAC 1 cut(s) 794
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 5516
AsiGI ACCGGT 1 cut(s) 1717
Asp700I GAANNNNTTC 4 cut(s) 872, 3474, 4174, 5277
AspLEI GCGC 6 cut(s) 422, 960, 1801, 2449, 2681, 3664
AsuC2I CCSGG 2 cut(s) 3960, 5869
AsuHPI GGTGA 7 cut(s) 1145, 1830, 3752, 4295, 4561, 5431, 5728
AsuNHI GCTAGC 1 cut(s) 2582
AvaI CYCGRG 2 cut(s) 80, 1516
AvaII GGWCC 6 cut(s) 2072, 2512, 2551, 2962, 2968, 3962
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 2 cut(s) 798, 850
BalI TGGCCA 6 cut(s) 152, 2612, 3578, 4244, 4275, 5680
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 109, 4128, 4913
BanII GRGCYC 4 cut(s) 2176, 3841, 5019, 5355
BauI CACGAG 1 cut(s) 5700
BbrPI CACGTG 2 cut(s) 341, 4022
BbsI GAAGAC 4 cut(s) 656, 4362, 4615, 5453
Bbv12I GWGCWC 6 cut(s) 771, 798, 1979, 2830, 4679, 5019
BceAI ACGGC 3 cut(s) 97, 2673, 2724
BcgI CGANNNNNNTGC 2 cut(s) 2208, 2242
BciVI GTATCC 2 cut(s) 62, 2194
BclI TGATCA 5 cut(s) 820, 3034, 3742, 4599, 5505
BcnI CCSGG 2 cut(s) 3960, 5869
BcoDI GTCTC 1 cut(s) 1952
BfmI CTRYAG 5 cut(s) 719, 1906, 4588, 4975, 5576
BfuAI ACCTGC 1 cut(s) 3973
BfuI GTATCC 2 cut(s) 62, 2194
BglI GCCNNNNNGGC 1 cut(s) 46
BglII AGATCT 2 cut(s) 2297, 5043
BmcAI AGTACT 1 cut(s) 4594
Bme18I GGWCC 6 cut(s) 2072, 2512, 2551, 2962, 2968, 3962
BmeRI GACNNNNNGTC 1 cut(s) 4506
BmeT110I CYCGRG 2 cut(s) 80, 1516
BmgBI CACGTC 1 cut(s) 248
BmrI ACTGGG 3 cut(s) 1225, 4841, 5735
BmtI GCTAGC 1 cut(s) 2586
BmuI ACTGGG 3 cut(s) 1225, 4841, 5735
BpiI GAAGAC 4 cut(s) 656, 4362, 4615, 5453
BpmI CTGGAG 3 cut(s) 2951, 3090, 3222
Bpu10I CCTNAGC 1 cut(s) 1680
BpuEI CTTGAG 3 cut(s) 1913, 2792, 4665
BpuMI CCSGG 2 cut(s) 3960, 5869
Bsa29I ATCGAT 1 cut(s) 1390
BsaAI YACGTR 3 cut(s) 341, 2099, 4022
BsaBI GATNNNNATC 1 cut(s) 3039
BsaWI WCCGGW 4 cut(s) 1284, 1717, 2344, 3540
BsaXI ACNNNNNCTCC 4 cut(s) 1098, 1128, 4442, 4472
Bse118I RCCGGY 1 cut(s) 1717
Bse1I ACTGG 6 cut(s) 1231, 2113, 3917, 4847, 5620, 5741
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 6 cut(s) 2312, 2629, 2674, 3671, 4402, 5370
Bse8I GATNNNNATC 1 cut(s) 3039
BseCI ATCGAT 1 cut(s) 1390
BseJI GATNNNNATC 1 cut(s) 3039
BseMI GCAATG 6 cut(s) 2312, 2629, 2674, 3671, 4402, 5370
BseMII CTCAG 7 cut(s) 1775, 3755, 3854, 4267, 4519, 5301, 5472
BseNI ACTGG 6 cut(s) 1231, 2113, 3917, 4847, 5620, 5741
BseRI GAGGAG 3 cut(s) 2498, 3311, 5276
BseSI GKGCMC 2 cut(s) 798, 850
BseX3I CGGCCG 1 cut(s) 402
BseYI CCCAGC 2 cut(s) 1082, 1853
BsgI GTGCAG 1 cut(s) 1326
Bsh1236I CGCG 1 cut(s) 420
Bsh1285I CGRYCG 1 cut(s) 405
BshNI GGYRCC 3 cut(s) 109, 4128, 4913
BshTI ACCGGT 1 cut(s) 1717
BshVI ATCGAT 1 cut(s) 1390
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 6 cut(s) 771, 798, 1979, 2830, 4679, 5019
BsiHKCI CYCGRG 2 cut(s) 80, 1516
BsiSI CCGG 9 cut(s) 23, 910, 1285, 1718, 2345, 3541, 3959, 5500, 5869
BsiWI CGTACG 1 cut(s) 5326
BslFI GGGAC 3 cut(s) 2377, 3975, 4885
BsmAI GTCTC 1 cut(s) 1952
BsmBI CGTCTC 1 cut(s) 1952
BsmFI GGGAC 3 cut(s) 2377, 3975, 4885
BsoBI CYCGRG 2 cut(s) 80, 1516
Bsp19I CCATGG 1 cut(s) 3162
BspCNI CTCAG 7 cut(s) 1774, 3756, 3853, 4266, 4518, 5302, 5473
BspDI ATCGAT 1 cut(s) 1390
BspFNI CGCG 1 cut(s) 420
BspHI TCATGA 3 cut(s) 2088, 3771, 5442
BspMAI CTGCAG 2 cut(s) 723, 5580
BspMI ACCTGC 1 cut(s) 3973
BspOI GCTAGC 1 cut(s) 2586
BspQI GCTCTTC 4 cut(s) 279, 651, 2835, 5801
BspT107I GGYRCC 3 cut(s) 109, 4128, 4913
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 6 cut(s) 2312, 2629, 2674, 3671, 4402, 5370
BsrFI RCCGGY 1 cut(s) 1717
BsrI ACTGG 6 cut(s) 1231, 2113, 3917, 4847, 5620, 5741
BssAI RCCGGY 1 cut(s) 1717
BssNAI GTATAC 1 cut(s) 1734
BssSI CACGAG 1 cut(s) 5700
BssT1I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3162, 4335
Bst1107I GTATAC 1 cut(s) 1734
Bst2BI CACGAG 1 cut(s) 5700
Bst6I CTCTTC 7 cut(s) 279, 651, 1134, 2835, 3362, 4713, 5801
BstAPI GCANNNNNTGC 3 cut(s) 949, 2444, 4478
BstBAI YACGTR 3 cut(s) 341, 2099, 4022
BstDSI CCRYGG 3 cut(s) 2998, 3162, 5212
BstEII GGTNACC 3 cut(s) 927, 2798, 5113
BstENI CCTNNNNNAGG 1 cut(s) 83
BstFNI CGCG 1 cut(s) 420
BstHHI GCGC 6 cut(s) 422, 960, 1801, 2449, 2681, 3664
BstMAI GTCTC 1 cut(s) 1952
BstMCI CGRYCG 1 cut(s) 405
BstNSI RCATGY 4 cut(s) 1462, 2674, 2929, 3098
BstPI GGTNACC 3 cut(s) 927, 2798, 5113
BstSFI CTRYAG 5 cut(s) 719, 1906, 4588, 4975, 5576
BstSLI GKGCMC 2 cut(s) 798, 850
BstSNI TACGTA 1 cut(s) 2099
BstUI CGCG 1 cut(s) 420
BstV2I GAAGAC 4 cut(s) 656, 4362, 4615, 5453
BstX2I RGATCY 5 cut(s) 229, 2297, 2488, 2589, 5043
BstXI CCANNNNNNTGG 5 cut(s) 3387, 3719, 4889, 5048, 5619
BstYI RGATCY 5 cut(s) 229, 2297, 2488, 2589, 5043
BstZ17I GTATAC 1 cut(s) 1734
BstZI CGGCCG 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 1390
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 2 cut(s) 62, 2194
BsuTUI ATCGAT 1 cut(s) 1390
BtgI CCRYGG 3 cut(s) 2998, 3162, 5212
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 1 cut(s) 248
BtsIMutI CAGTG 9 cut(s) 106, 1238, 1278, 2120, 2567, 4602, 4620, 5088, 5482
BveI ACCTGC 1 cut(s) 3973
CaiI CAGNNNCTG 3 cut(s) 1313, 1697, 3680
CciI TCATGA 3 cut(s) 2088, 3771, 5442
CfoI GCGC 6 cut(s) 422, 960, 1801, 2449, 2681, 3664
Cfr10I RCCGGY 1 cut(s) 1717
ClaI ATCGAT 1 cut(s) 1390
CseI GACGC 3 cut(s) 70, 496, 1558
CsiI ACCWGGT 2 cut(s) 3933, 4644
Csp6I GTAC 9 cut(s) 1996, 2096, 3516, 3535, 3920, 4593, 5038, 5327, 5485
CspAI ACCGGT 1 cut(s) 1717
CviQI GTAC 9 cut(s) 1996, 2096, 3516, 3535, 3920, 4593, 5038, 5327, 5485
DraI TTTAAA 1 cut(s) 471
DriI GACNNNNNGTC 1 cut(s) 4506
EaeI YGGCCR 8 cut(s) 150, 402, 1295, 2610, 3576, 4242, 4273, 5678
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 7 cut(s) 279, 651, 1134, 2835, 3362, 4713, 5801
Eam1105I GACNNNNNGTC 1 cut(s) 4506
EarI CTCTTC 7 cut(s) 279, 651, 1134, 2835, 3362, 4713, 5801
EciI GGCGGA 1 cut(s) 1657
Ecl136II GAGCTC 1 cut(s) 5017
EclXI CGGCCG 1 cut(s) 402
Eco105I TACGTA 1 cut(s) 2099
Eco130I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3162, 4335
Eco24I GRGCYC 4 cut(s) 2176, 3841, 5019, 5355
Eco47I GGWCC 6 cut(s) 2072, 2512, 2551, 2962, 2968, 3962
Eco52I CGGCCG 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 5017
Eco57I CTGAAG 4 cut(s) 2399, 3386, 3542, 3894
Eco72I CACGTG 2 cut(s) 341, 4022
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 2 cut(s) 80, 1516
Eco91I GGTNACC 3 cut(s) 927, 2798, 5113
EcoICRI GAGCTC 1 cut(s) 5017
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 3962
EcoO65I GGTNACC 3 cut(s) 927, 2798, 5113
EcoRI GAATTC 4 cut(s) 2813, 3214, 3622, 4817
EcoT14I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3162, 4335
EcoT22I ATGCAT 2 cut(s) 1971, 2676
EcoT38I GRGCYC 4 cut(s) 2176, 3841, 5019, 5355
ErhI CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3162, 4335
Esp3I CGTCTC 1 cut(s) 1952
FalI AAGNNNNNCTT 8 cut(s) 3459, 3491, 4162, 4194, 4704, 4736, 5262, 5294
FaqI GGGAC 3 cut(s) 2377, 3975, 4885
FauI CCCGC 3 cut(s) 10, 2402, 5837
FauNDI CATATG 2 cut(s) 1053, 3697
FbaI TGATCA 5 cut(s) 820, 3034, 3742, 4599, 5505
FblI GTMKAC 1 cut(s) 1733
FriOI GRGCYC 4 cut(s) 2176, 3841, 5019, 5355
FspI TGCGCA 1 cut(s) 2448
GlaI GCGC 6 cut(s) 421, 959, 1800, 2448, 2680, 3663
GsaI CCCAGC 2 cut(s) 1086, 1857
GsuI CTGGAG 3 cut(s) 2951, 3090, 3222
HapII CCGG 9 cut(s) 23, 910, 1285, 1718, 2345, 3541, 3959, 5500, 5869
HgaI GACGC 3 cut(s) 70, 496, 1558
HhaI GCGC 6 cut(s) 422, 960, 1801, 2449, 2681, 3664
Hin6I GCGC 6 cut(s) 420, 958, 1799, 2447, 2679, 3662
HinP1I GCGC 6 cut(s) 420, 958, 1799, 2447, 2679, 3662
HincII GTYRAC 2 cut(s) 1408, 2779
HindII GTYRAC 2 cut(s) 1408, 2779
HindIII AAGCTT 9 cut(s) 387, 869, 1447, 1560, 1595, 3546, 3953, 4074, 5643
HpaI GTTAAC 1 cut(s) 1408
HpaII CCGG 9 cut(s) 23, 910, 1285, 1718, 2345, 3541, 3959, 5500, 5869
HphI GGTGA 7 cut(s) 1145, 1830, 3752, 4295, 4561, 5431, 5728
Hpy99I CGWCG 3 cut(s) 10, 13, 2265
HpyCH4IV ACGT 8 cut(s) 247, 283, 340, 1179, 2098, 4021, 5296, 5329
HpySE526I ACGT 8 cut(s) 247, 283, 340, 1179, 2098, 4021, 5296, 5329
HspAI GCGC 6 cut(s) 420, 958, 1799, 2447, 2679, 3662
Ksp22I TGATCA 5 cut(s) 820, 3034, 3742, 4599, 5505
KspAI GTTAAC 1 cut(s) 1408
LguI GCTCTTC 4 cut(s) 279, 651, 2835, 5801
MabI ACCWGGT 2 cut(s) 3933, 4644
MaeII ACGT 8 cut(s) 247, 283, 340, 1179, 2098, 4021, 5296, 5329
MbiI CCGCTC 1 cut(s) 17
MfeI CAATTG 5 cut(s) 3900, 4041, 4575, 4832, 4838
MflI RGATCY 5 cut(s) 229, 2297, 2488, 2589, 5043
MlsI TGGCCA 6 cut(s) 152, 2612, 3578, 4244, 4275, 5680
MluNI TGGCCA 6 cut(s) 152, 2612, 3578, 4244, 4275, 5680
MlyI GAGTC 5 cut(s) 1102, 1159, 3164, 3505, 5686
MmeI TCCRAC 5 cut(s) 207, 329, 816, 2099, 3201
Mox20I TGGCCA 6 cut(s) 152, 2612, 3578, 4244, 4275, 5680
Mph1103I ATGCAT 2 cut(s) 1971, 2676
MroXI GAANNNNTTC 4 cut(s) 872, 3474, 4174, 5277
MscI TGGCCA 6 cut(s) 152, 2612, 3578, 4244, 4275, 5680
MslI CAYNNNNRTG 6 cut(s) 3167, 3698, 4281, 4421, 4887, 4911
Msp20I TGGCCA 6 cut(s) 152, 2612, 3578, 4244, 4275, 5680
MspA1I CMGCKG 4 cut(s) 46, 58, 71, 1694
MspI CCGG 9 cut(s) 23, 910, 1285, 1718, 2345, 3541, 3959, 5500, 5869
MunI CAATTG 5 cut(s) 3900, 4041, 4575, 4832, 4838
MvnI CGCG 1 cut(s) 420
NciI CCSGG 2 cut(s) 3960, 5869
NcoI CCATGG 1 cut(s) 3162
NdeI CATATG 2 cut(s) 1053, 3697
NheI GCTAGC 1 cut(s) 2582
NmuCI GTSAC 7 cut(s) 1162, 1836, 1935, 2023, 2938, 3651, 5545
NsbI TGCGCA 1 cut(s) 2448
NsiI ATGCAT 2 cut(s) 1971, 2676
NspI RCATGY 4 cut(s) 1462, 2674, 2929, 3098
OliI CACNNNNGTG 1 cut(s) 3698
PagI TCATGA 3 cut(s) 2088, 3771, 5442
PciI ACATGT 1 cut(s) 3094
PciSI GCTCTTC 4 cut(s) 279, 651, 2835, 5801
PcsI WCGNNNNNNNCGW 2 cut(s) 253, 5857
PdmI GAANNNNTTC 4 cut(s) 872, 3474, 4174, 5277
Pfl23II CGTACG 1 cut(s) 5326
PflMI CCANNNNNTGG 2 cut(s) 1127, 1382
PfoI TCCNGGA 3 cut(s) 2919, 3958, 5867
PinAI ACCGGT 1 cut(s) 1717
PleI GAGTC 5 cut(s) 1101, 1159, 3163, 3505, 5686
PmaCI CACGTG 2 cut(s) 341, 4022
PmlI CACGTG 2 cut(s) 341, 4022
PpsI GAGTC 5 cut(s) 1101, 1159, 3163, 3505, 5686
Ppu21I YACGTR 3 cut(s) 341, 2099, 4022
PpuMI RGGWCCY 1 cut(s) 3962
PscI ACATGT 1 cut(s) 3094
PshBI ATTAAT 1 cut(s) 5516
PsiI TTATAA 2 cut(s) 1577, 2495
Psp124BI GAGCTC 1 cut(s) 5019
Psp5II RGGWCCY 1 cut(s) 3962
PspCI CACGTG 2 cut(s) 341, 4022
PspEI GGTNACC 3 cut(s) 927, 2798, 5113
PspFI CCCAGC 2 cut(s) 1082, 1853
PspLI CGTACG 1 cut(s) 5326
PspPPI RGGWCCY 1 cut(s) 3962
PstI CTGCAG 2 cut(s) 723, 5580
PstNI CAGNNNCTG 3 cut(s) 1313, 1697, 3680
PsuI RGATCY 5 cut(s) 229, 2297, 2488, 2589, 5043
PvuII CAGCTG 2 cut(s) 71, 1694
RsaI GTAC 9 cut(s) 1997, 2097, 3517, 3536, 3921, 4594, 5039, 5328, 5486
RsaNI GTAC 9 cut(s) 1996, 2096, 3516, 3535, 3920, 4593, 5038, 5327, 5485
RseI CAYNNNNRTG 6 cut(s) 3167, 3698, 4281, 4421, 4887, 4911
SacI GAGCTC 1 cut(s) 5019
SapI GCTCTTC 4 cut(s) 279, 651, 2835, 5801
SbfI CCTGCAGG 1 cut(s) 5580
ScaI AGTACT 1 cut(s) 4594
SchI GAGTC 5 cut(s) 1102, 1159, 3164, 3505, 5686
SdaI CCTGCAGG 1 cut(s) 5580
SexAI ACCWGGT 2 cut(s) 3933, 4644
SfcI CTRYAG 5 cut(s) 719, 1906, 4588, 4975, 5576
SinI GGWCC 6 cut(s) 2072, 2512, 2551, 2962, 2968, 3962
SmiMI CAYNNNNRTG 6 cut(s) 3167, 3698, 4281, 4421, 4887, 4911
SmlI CTYRAG 3 cut(s) 1892, 2771, 4680
SmoI CTYRAG 3 cut(s) 1892, 2771, 4680
SnaBI TACGTA 1 cut(s) 2099
Sse8387I CCTGCAGG 1 cut(s) 5580
SspI AATATT 5 cut(s) 1536, 1590, 2235, 5285, 5520
SstI GAGCTC 1 cut(s) 5019
StyI CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3162, 4335
TaiI ACGT 8 cut(s) 250, 286, 343, 1182, 2101, 4024, 5299, 5332
TaqII GACCGA 1 cut(s) 4433
TatI WGTACW 4 cut(s) 1995, 3534, 3919, 4592
TauI GCSGC 6 cut(s) 37, 40, 43, 49, 92, 404
TscAI CASTG 9 cut(s) 106, 1238, 1278, 2120, 2567, 4609, 4620, 5095, 5482
TseFI GTSAC 7 cut(s) 1162, 1836, 1935, 2023, 2938, 3651, 5545
Tsp45I GTSAC 7 cut(s) 1162, 1836, 1935, 2023, 2938, 3651, 5545
TspGWI ACGGA 8 cut(s) 2249, 2293, 2798, 3015, 4376, 4538, 4909, 5314
TspRI CASTG 9 cut(s) 106, 1238, 1278, 2120, 2567, 4609, 4620, 5095, 5482
Van91I CCANNNNNTGG 2 cut(s) 1127, 1382
VneI GTGCAC 1 cut(s) 794
VpaK11BI GGWCC 6 cut(s) 2072, 2512, 2551, 2962, 2968, 3962
VspI ATTAAT 1 cut(s) 5516
XagI CCTNNNNNAGG 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 1612
XceI RCATGY 4 cut(s) 1462, 2674, 2929, 3098
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 3319, 5392
XmiI GTMKAC 1 cut(s) 1733
XmnI GAANNNNTTC 4 cut(s) 872, 3474, 4174, 5277
ZrmI AGTACT 1 cut(s) 4594
Zsp2I ATGCAT 2 cut(s) 1971, 2676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.