Rh5DG556800

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0005959
Physical Location & Seq
Forward (+)
85852752 .. 85868000
15249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG556800.1

Sequence Viewer

Length: 5865 bp
ATGTCGTCGTCGAGAGCGGGGCCGGATCAGGGGCCGCCGCCGCAGCGGCGTATCCAGCGGACGCAGACAGCTGGAAACCTCGGGGAGGCGGCGTTCGACAGTGAGGTGGTGCCGTCATCGCTTGTTGAAATCGCTCCCATTCTTCGTGTGGCCAACGAGGTCGAATCGCACAATCCCAGAGTTGCTTATCTATGTCGATTTTATGCCTTTGAGAAAGCTCATAGGTTGGATCCCACTTCTAGTGGACGTGGTGTTCGTCAGTTCAAAACTGCTCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACTCTTATGGGAAGGGTAAAAAAAAGTGACGCACGTGAAATGCAGAGCTTCTATCAACACTATTACAAAAAATATATTCAAGCTTTGCAAAATGCGGCCGATAAAGCTGACCGCGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTATTTGAGGTTTTAAAGGCTGTTAATATGACGCAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGATAAAGTTGCAGAAAAGACAGAGCTATTGGTTCCTTACAATATCCTTCCACTTGATCCTGATAGTGTAAATCAAGCAATTATGAGATACCCAGAGATTCAAGCTGCTGTTGTTGCTCTTCGTAACACCAGGGGTCTTCCTTGGCCTAAGGAATACAAAAAGAAAAAAGACGAGGATGTTCTTGATTGGCTGCAGTCAATGTTTGGGTTTCAGAAGGACAATGTGGCGAATCAACGGGAGCACCTGATATTGTTACTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAGCCCAAGTTGGATGACCGTGCCCTGACAGAAGTGATGAAGAAGCTTTTCAAGAATTACAAAAAATGGTGCAAGTTCTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACCATACAGCAAGAGGTGCAACAGCGCAAGCTGCTTTACATGGGTCTATATCTTTTGATATGGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGTTACATTTATCATCATATGGCATTTGAATTGTATGGTATGTTAGCTGGCAATGTGAGTCCAATGACAGGAGAAAATGTGAAGCCAGCCTATGGAGGTGAAGAAGAGGCTTTCTTGAAAAAAGTTGTGACTCCTATCTATAACGTGATTGCCAGGGAAGCTGATAGGAGCAAAAGAGGGGAATCAAAGCATTCCCAGTGGAGGAACTATGATGATATAAATGAATACTTTTGGTCAGTGGATTGTTTCCGGTTGGGTTGGCCGATGCGTGCAGATGCTGATTTCTTTTGCTGCTCTAGTGAACAACGTCGTTTTGATAAAAATAGTGAGGATAACAAACCAGCTAGTGGAGATCGATGGGTGGGGAAAGTTAACTTTGTTGAGATACGGTCATTCTGGCATATCTTTAGAAGCTTTGACCGCATGTGGAGTTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAATGGCTCGGGCCAACCCACATCAATATTTTCTGCTGATGTATTCAAGAAAGCTTTGAGTGTCTTTATAACTGCTGCAATATTGAAGCTTGGACAAGCTGTTCTAGATGTAATTCTTAGCTGGAAGTCTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATATATTGCTAAGGTCATTTCAGCTGCTGCGTGGGTAATAATTCTACCAGTTACTTATGCGTATACTTGGGAAAACCCTCCTGGGTTTGCTCAGACCATTAAAGGTTGGTTTGGCAATAGTAAAAATGCGCCCTCCTTGTTCATCTTGGCTGTTGTTATCTACTTGTCACCGAATATGCTGGCTGGGGTGTTGTTTCTTTTCCCATTTATTCGCCGATTCCTTGAGAGATCAAACTATAGGATTGTGATGCTTATGATGTGGTGGTCACAGCCTCGTCTCTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCTCTTTTCAAGTACACGATGTTTTGGGTTCTCCTTGTAGTCACAAAGTTGGCATTCAGTTACTATATAGAGATAAAGCCTTTAGTGGGTCCAACAAAAGCCATCATGAATGTACGTATAACTAATTTCCAGTGGCATGAGTTCTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTGGTCTATTTTATGGATACCCAGATTTGGTATGCAATATACTCCACAATATTCGGAGGAATTTATGGTGCATTCCGTCGCCTTGGAGAGATACGGACACTAGGAATGCTAAGATCTCGCTTTGAATCATTGCCTGGTGCTTTTAACGCCCGTTTAATTCCAGCGGACAAGAGTGAGCCAAAGAAGAAAGGACTGAAGGCTACATTGTCCCGCACCTTTGGTCATGTTGAAGGTAGTAAAGACAAACAGGCTGCAAGGTTTGCGCAATTATGGAACAAAATAATAAGTAGTTTCAGAGAGGAGGATCTTATAAACAAAAGGGAAATGAACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGAAATGGACCTCATACAGTGGCCTCCATTTTTACTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGCTAACAAAAAGAATCCTGGCTGATGAATACATGCATTGTGCTGTTCGTGAATGCTATGCTTCATTTAAGAACATAATTAAGTTTCTGGTTCAGGGGGATCGTGAGAAAGAGGTTATAAATTTCATATTTTCTGAGGTTGACAAGCACATAGAAGATGGTGACCTGATCCGTGAATTCAAGATGAGTGCTCTTCCTAGCCTCTATGACCACTTTGTTAGCCTTATCAACTTTTTGGGAAAGAATAGTCAGGACGACAGGGATCAAGTTGTGATTCTATTCCAGGACATGCTGGAGGTAGTGACTAGAGATATAATGATGGAGGACCATATAAACAGCTTGGTAGATTCAGTCCACGGAGTCTCAGGCCATGAGGGGATGATGCCCCTTGATCAACATCAACAGCATCAGTTGTTTGCATCTGCTGGAGCTATCAACTTTCCTCTTACACATGTAACAGAGGCTTGGAAGGAGAAGATTAACCGTCTTTTTCTATTACTTACCACAAAGGAGTCTGCCATGGACGTGCCATCCAACTTAGAAGCTAGAAGGCGAATTTCTTTCTTCTCGAATTCATTGTTTATGGATATGCCTCCAGCACCAAAAGTTCGCAATATGCTTTCTTTCTCTGTTTTAACTCCTTACTACAACGAGGAGGTTCTCTTTTCCATTGACGGTCTGGAAAAACCGAATGAAGATGGTGTTTCTATTCTCTTCTACTTGCAAAAGATTTTCCCAGATGAATGGAACAACTTTCTTGAGCGTGTGAATTGCAACAACGAAGAGGAACTAAAAGGTTCTGATGAATTGGAAGAAGATCTTCGCCTATGGGCATCATATAGAGGCCAAACTTTGACTCGTACTGTAAGAGGGATGATGTACTACCGGAAAGCTTTGGAGCTTCAGGCTTTTCTGGATATGGCCAAAGAGGAGGATTTAATGGAAGGCTATAAGGCCATAGAGTTGAATTCAGAAGATCAATCAAAGGAGGGAAGATCACTATGGACGCAATGTCAAGCAGTAGCTGATATGAAATTCACATATGTGGTTTCATGCCAAAAGTATGGTATTCAGAAGCGATCTGGTGATCTTCGTGCACAGGACATTCTGAGGCTCATGACAACATACCCATCATTACGAGTGGCATATATTGATGAGGTTGAAGAACCTAGCAAAGATAGGTCTCAGAAGATAAACCAAAAGGCTTATTATTCTACTTTGGTAAAGGCTGCTATGCCAAAGTCAATTGATTCTTCAGAGCCAGTACAAAATCTTGACCAGGTTATTTATCGCATAAAACTTCCAGGACCTGCTATTTTGGGAGAGGGAAAGCCAGAAAATCAAAATCATGCCATTATTTTCACGCGTGGAGAAGGCTTGCAAACAATTGACATGAACCAGGATAACTACATGGAAGAAGCTTTGAAAATGAGGAATTTGCTACAAGAATTTCTTAAGCATGATGGCGTGAGGCACCCTACAATTCTTGGACTTAGGGAGCATATATTTACAGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAGAACAGTTTTGTGACTATTGGTCAAAGACTATTGGCCAACCCACTCAGGGTTCGATTTCATTATGGGCATCCCGATGTATTTGATAGGCTCTTTCACCTTAGTAGAGGGGGTGTCAGTAAGGCATCCAAGGTTATCAATTTGAGTGAAGACATTTTTGCTGGCTTTAATTCCACTCTCCGTGAAGGCAATGTAACTCATCACGAATACATACAAGTGGGGAAGGGGAGAGATGTCGGTCTTAACCAAATCTCCATGTTTGAGGCAAAAATTGCTAATGGCAACGGAGAGCAGACACTCAGTCGAGATATATACCGACTTGGACATCGTTTTGACTTTTTCCGTATGTTGTCATGTTATTTCACCACAATTGGTTTCTACTTCAGTACTCTGATCACTGTGCTTACAGTGTATGTCTTCCTTTATGGTCGCCTCTACCTGGTTCTTAGTGGACTTGAAGAAGGTTTGAACACTCAAGCAGCAATTCGAGATAATAAGCCTCTTCAAGTTGCTCTCGCTTCTCAATCATTTGTTCAAATAGGTTTTTTGATGGCTTTGCCTATGTTGATGGAAATTGGCTTGGAAAAGGGTTTCCGAACTGCATTAAGTGAATTTGTGTTGATGCAATTGCAACTGGCCCCAGTATTTTTCACATTCTCACTTGGGACAAAGACCCACTATTATGGACGGACATTACTGCATGGTGGTGCCAAATATAGATCTACAGGTCGTGGGTTTGTGGTGTTCCATGCCAAGTTTGCCGACAACTATAGGCTCTACTCCCGCAGCCACTTTGTTAAGGGTATTGAGCTCTTGATTTTACTTGTGGTGTACCAAATCTTTGGTCATACTTACAGAAGTGCTGTTGCCTACATTTTGATCACTGTATCTATGTGGTTTATGGTGGTTACCTGGCTTTTTGCTCCCTTTCTGTTCAATCCATCTGGTTTTGAGTGGCAAAAGATTGTTGATGATTGGACTGATTGGAATAAGTGGATAAGCAACCGTGGAGGTATAGGTGTTCCACCTGAAAAAAGTTGGGAATCATGGTGGGAGGAGGAACAAGAACATCTTCGATATTCCGGAAAACGTGGCATTGTAGTTGAGATACTGCTATCTGTACGATTCTTTATCTATCAGTATGGGCTCGTATATCACTTAAACATTGCAAAGAAAACCAAGAGTGTCCTGGTCTATGGTATCTCATGGCTGGTGATCGTCCTTATTTTGTTTGTCATGAAGACTGTATCTGTTGGTAGGAGAAAGTTCAGTGCTGAGTACCAACTTGTATTCCGGCTGATCAAGGGATTAATATTCATTACTTTTGTGTCCATTTTGGTCACTTTGATTGTGCTGCCTCACATGACCCTGCAGGACATCATTGTTTGCATTCTTGCTTTCATGCCAACTGGTTGGGGGATGCTTATGATTGCCCAAGCTTGCAAAGGCTTGGTTCATAAAGCTGGTTTATGGCCATCAGTTCGGACTCTTGCCCGTGGCTTTGAGATAGTTATGGGCTTGCTTCTGTTCACCCCAGTTGCATTCTTGGCCTGGTTTCCTTTTGTTTCGGAATTTCAAACTCGTATGCTCTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCTCGTATTCTTGGCGGACAACGCAAGGATCGTTCATCCCGGAACAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1954

Amino Acids

225.53

Weight (kDa)

9.17

Isoelectric Point (pI)

42.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 44 - 171 1.9e-17 Vta1 like
FKS1_dom1 PF14288 321 - 433 3.4e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 856 - 1048 5.6e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1055 - 1848 1.8e-238 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1577, 2495, 2756
Acc16I TGCGCA 1 cut(s) 2448
Acc36I ACCTGC 1 cut(s) 3955
AccB1I GGYRCC 3 cut(s) 109, 4110, 4895
AccB7I CCANNNNNTGG 2 cut(s) 1127, 1382
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 1733
AccII CGCG 2 cut(s) 420, 4003
AccIII TCCGGA 1 cut(s) 5270
AcoI YGGCCR 7 cut(s) 150, 402, 1295, 2610, 3558, 4224, 5660
AcuI CTGAAG 4 cut(s) 2399, 3524, 3876, 4555
AcvI CACGTG 1 cut(s) 341
AfaI GTAC 9 cut(s) 1997, 2097, 3499, 3518, 3903, 4576, 5021, 5310, 5468
AflII CTTAAG 1 cut(s) 4091
AflIII ACRYGT 2 cut(s) 3088, 4001
AhdI GACNNNNNGTC 3 cut(s) 3648, 4209, 4488
AjiI CACGTC 2 cut(s) 248, 3163
AleI CACNNNNGTG 1 cut(s) 3680
AloI GAACNNNNNNTCC 6 cut(s) 77, 109, 237, 269, 1593, 1625
Alw21I GWGCWC 6 cut(s) 771, 798, 1979, 2830, 3736, 5001
Alw26I GTCTC 3 cut(s) 1952, 3004, 3825
Alw44I GTGCAC 2 cut(s) 794, 3732
AlwNI CAGNNNCTG 4 cut(s) 1313, 1697, 3662, 3947
Ama87I CYCGRG 2 cut(s) 80, 1516
Aor13HI TCCGGA 1 cut(s) 5270
ApaLI GTGCAC 2 cut(s) 794, 3732
ArsI GACNNNNNNTTYG 4 cut(s) 237, 269, 5017, 5049
AseI ATTAAT 1 cut(s) 5498
Asp700I GAANNNNTTC 4 cut(s) 872, 3456, 4156, 5259
AspLEI GCGC 4 cut(s) 422, 960, 1801, 2449
AspS9I GGNCC 9 cut(s) 20, 32, 907, 1519, 2072, 2551, 2962, 3944, 4825
AsuC2I CCSGG 1 cut(s) 5851
AsuHPI GGTGA 8 cut(s) 1145, 1830, 2810, 3734, 4277, 4543, 5413, 5710
AsuNHI GCTAGC 1 cut(s) 2582
AvaI CYCGRG 2 cut(s) 80, 1516
AvaII GGWCC 4 cut(s) 2072, 2551, 2962, 3944
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 3 cut(s) 798, 850, 3736
BalI TGGCCA 5 cut(s) 152, 2612, 3560, 4226, 5662
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 109, 4110, 4895
BanII GRGCYC 3 cut(s) 2176, 5001, 5337
BbrPI CACGTG 1 cut(s) 341
BbsI GAAGAC 4 cut(s) 656, 4344, 4597, 5435
Bbv12I GWGCWC 6 cut(s) 771, 798, 1979, 2830, 3736, 5001
BceAI ACGGC 1 cut(s) 97
BciVI GTATCC 2 cut(s) 62, 2194
BclI TGATCA 5 cut(s) 820, 3028, 4581, 5067, 5487
BcnI CCSGG 1 cut(s) 5851
BcoDI GTCTC 3 cut(s) 1952, 3004, 3825
BfmI CTRYAG 5 cut(s) 719, 1906, 4911, 4957, 5558
BfrI CTTAAG 1 cut(s) 4091
BfuAI ACCTGC 1 cut(s) 3955
BfuI GTATCC 2 cut(s) 62, 2194
BglI GCCNNNNNGGC 1 cut(s) 46
BglII AGATCT 3 cut(s) 2297, 3454, 4907
BmcAI AGTACT 1 cut(s) 4576
Bme18I GGWCC 4 cut(s) 2072, 2551, 2962, 3944
BmeRI GACNNNNNGTC 3 cut(s) 3648, 4209, 4488
BmeT110I CYCGRG 2 cut(s) 80, 1516
BmgBI CACGTC 2 cut(s) 248, 3163
BmgT120I GGNCC 9 cut(s) 20, 32, 907, 1519, 2072, 2551, 2962, 3944, 4825
BmrI ACTGGG 3 cut(s) 1225, 4823, 5717
BmtI GCTAGC 1 cut(s) 2586
BmuI ACTGGG 3 cut(s) 1225, 4823, 5717
BpiI GAAGAC 4 cut(s) 656, 4344, 4597, 5435
BpmI CTGGAG 3 cut(s) 2951, 3084, 3216
Bpu10I CCTNAGC 1 cut(s) 1680
BpuEI CTTGAG 3 cut(s) 1913, 3416, 4647
BpuMI CCSGG 1 cut(s) 5851
Bsa29I ATCGAT 1 cut(s) 1390
BsaAI YACGTR 2 cut(s) 341, 2099
BsaBI GATNNNNATC 1 cut(s) 3033
BsaI GGTCTC 1 cut(s) 3825
BsaWI WCCGGW 3 cut(s) 1284, 3522, 5270
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 1098, 1128, 4424, 4454
Bse1I ACTGG 8 cut(s) 1231, 1718, 2113, 3899, 4827, 4829, 5602, 5723
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 6 cut(s) 1093, 2312, 2629, 3653, 4384, 5352
Bse8I GATNNNNATC 1 cut(s) 3033
BseAI TCCGGA 1 cut(s) 5270
BseCI ATCGAT 1 cut(s) 1390
BseGI GGATG 9 cut(s) 709, 844, 3021, 3167, 3516, 4258, 4313, 5613, 5846
BseJI GATNNNNATC 1 cut(s) 3033
BseMI GCAATG 6 cut(s) 1093, 2312, 2629, 3653, 4384, 5352
BseMII CTCAG 8 cut(s) 1775, 2763, 3015, 3737, 3836, 4249, 4501, 5454
BseNI ACTGG 8 cut(s) 1231, 1718, 2113, 3899, 4827, 4829, 5602, 5723
BseRI GAGGAG 4 cut(s) 2498, 3305, 3581, 5258
BseSI GKGCMC 3 cut(s) 798, 850, 3736
BseX3I CGGCCG 1 cut(s) 402
BseYI CCCAGC 1 cut(s) 1853
BsgI GTGCAG 1 cut(s) 1326
Bsh1236I CGCG 2 cut(s) 420, 4003
Bsh1285I CGRYCG 1 cut(s) 405
BshNI GGYRCC 3 cut(s) 109, 4110, 4895
BshVI ATCGAT 1 cut(s) 1390
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 6 cut(s) 771, 798, 1979, 2830, 3736, 5001
BsiHKCI CYCGRG 2 cut(s) 80, 1516
BsiSI CCGG 7 cut(s) 23, 910, 1285, 3523, 5271, 5482, 5851
BslFI GGGAC 2 cut(s) 2377, 4867
BsmAI GTCTC 3 cut(s) 1952, 3004, 3825
BsmBI CGTCTC 1 cut(s) 1952
BsmFI GGGAC 2 cut(s) 2377, 4867
Bso31I GGTCTC 1 cut(s) 3825
BsoBI CYCGRG 2 cut(s) 80, 1516
Bsp1286I GDGCHC 9 cut(s) 771, 798, 850, 1979, 2176, 2830, 3736, 5001, 5337
Bsp13I TCCGGA 1 cut(s) 5270
Bsp19I CCATGG 1 cut(s) 3156
BspCNI CTCAG 8 cut(s) 1774, 2764, 3014, 3738, 3835, 4248, 4500, 5455
BspDI ATCGAT 1 cut(s) 1390
BspEI TCCGGA 1 cut(s) 5270
BspFNI CGCG 2 cut(s) 420, 4003
BspHI TCATGA 4 cut(s) 526, 2088, 3753, 5424
BspMAI CTGCAG 2 cut(s) 723, 5562
BspMI ACCTGC 1 cut(s) 3955
BspOI GCTAGC 1 cut(s) 2586
BspQI GCTCTTC 4 cut(s) 279, 651, 2835, 5783
BspT107I GGYRCC 3 cut(s) 109, 4110, 4895
BspTI CTTAAG 1 cut(s) 4091
BspTNI GGTCTC 1 cut(s) 3825
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 6 cut(s) 1093, 2312, 2629, 3653, 4384, 5352
BsrI ACTGG 8 cut(s) 1231, 1718, 2113, 3899, 4827, 4829, 5602, 5723
BssNAI GTATAC 1 cut(s) 1734
BssT1I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3156, 4317
Bst1107I GTATAC 1 cut(s) 1734
Bst6I CTCTTC 8 cut(s) 279, 651, 1134, 2835, 3356, 3414, 4695, 5783
BstAFI CTTAAG 1 cut(s) 4091
BstAPI GCANNNNNTGC 3 cut(s) 949, 2444, 4460
BstBAI YACGTR 2 cut(s) 341, 2099
BstDSI CCRYGG 4 cut(s) 2992, 3156, 5194, 5683
BstEII GGTNACC 3 cut(s) 927, 2798, 5095
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 9 cut(s) 709, 844, 3021, 3167, 3516, 4258, 4313, 5613, 5846
BstFNI CGCG 2 cut(s) 420, 4003
BstHHI GCGC 4 cut(s) 422, 960, 1801, 2449
BstMAI GTCTC 3 cut(s) 1952, 3004, 3825
BstMCI CGRYCG 1 cut(s) 405
BstNSI RCATGY 4 cut(s) 1462, 2674, 2929, 3092
BstPI GGTNACC 3 cut(s) 927, 2798, 5095
BstSFI CTRYAG 5 cut(s) 719, 1906, 4911, 4957, 5558
BstSLI GKGCMC 3 cut(s) 798, 850, 3736
BstSNI TACGTA 1 cut(s) 2099
BstUI CGCG 2 cut(s) 420, 4003
BstV2I GAAGAC 4 cut(s) 656, 4344, 4597, 5435
BstX2I RGATCY 6 cut(s) 229, 2297, 2488, 2589, 3454, 4907
BstXI CCANNNNNNTGG 5 cut(s) 3381, 3701, 4871, 5030, 5601
BstYI RGATCY 6 cut(s) 229, 2297, 2488, 2589, 3454, 4907
BstZ17I GTATAC 1 cut(s) 1734
BstZI CGGCCG 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 1390
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 2 cut(s) 62, 2194
BsuTUI ATCGAT 1 cut(s) 1390
BtgI CCRYGG 4 cut(s) 2992, 3156, 5194, 5683
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 248, 3163
BtsCI GGATG 9 cut(s) 709, 844, 3021, 3167, 3516, 4258, 4313, 5613, 5846
BtsIMutI CAGTG 9 cut(s) 106, 1238, 1278, 2120, 2567, 4584, 4602, 5070, 5464
BveI ACCTGC 1 cut(s) 3955
CaiI CAGNNNCTG 4 cut(s) 1313, 1697, 3662, 3947
CciI TCATGA 4 cut(s) 526, 2088, 3753, 5424
CfoI GCGC 4 cut(s) 422, 960, 1801, 2449
Cfr13I GGNCC 9 cut(s) 20, 32, 907, 1519, 2072, 2551, 2962, 3944, 4825
ClaI ATCGAT 1 cut(s) 1390
CseI GACGC 4 cut(s) 70, 344, 496, 3652
CsiI ACCWGGT 2 cut(s) 3915, 4626
Csp6I GTAC 9 cut(s) 1996, 2096, 3498, 3517, 3902, 4575, 5020, 5309, 5467
CviQI GTAC 9 cut(s) 1996, 2096, 3498, 3517, 3902, 4575, 5020, 5309, 5467
DraI TTTAAA 1 cut(s) 471
DriI GACNNNNNGTC 3 cut(s) 3648, 4209, 4488
EaeI YGGCCR 7 cut(s) 150, 402, 1295, 2610, 3558, 4224, 5660
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 8 cut(s) 279, 651, 1134, 2835, 3356, 3414, 4695, 5783
Eam1105I GACNNNNNGTC 3 cut(s) 3648, 4209, 4488
EarI CTCTTC 8 cut(s) 279, 651, 1134, 2835, 3356, 3414, 4695, 5783
EciI GGCGGA 2 cut(s) 1657, 5841
Ecl136II GAGCTC 1 cut(s) 4999
EclXI CGGCCG 1 cut(s) 402
Eco105I TACGTA 1 cut(s) 2099
Eco130I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3156, 4317
Eco24I GRGCYC 3 cut(s) 2176, 5001, 5337
Eco31I GGTCTC 1 cut(s) 3825
Eco47I GGWCC 4 cut(s) 2072, 2551, 2962, 3944
Eco52I CGGCCG 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 4999
Eco57I CTGAAG 4 cut(s) 2399, 3524, 3876, 4555
Eco72I CACGTG 1 cut(s) 341
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 2 cut(s) 80, 1516
Eco91I GGTNACC 3 cut(s) 927, 2798, 5095
EcoICRI GAGCTC 1 cut(s) 4999
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 3944
EcoO65I GGTNACC 3 cut(s) 927, 2798, 5095
EcoRI GAATTC 3 cut(s) 2813, 3208, 3604
EcoT14I CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3156, 4317
EcoT22I ATGCAT 2 cut(s) 1971, 2676
EcoT38I GRGCYC 3 cut(s) 2176, 5001, 5337
ErhI CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3156, 4317
Esp3I CGTCTC 1 cut(s) 1952
FalI AAGNNNNNCTT 8 cut(s) 3441, 3473, 4074, 4106, 4144, 4176, 5244, 5276
FaqI GGGAC 2 cut(s) 2377, 4867
FauI CCCGC 3 cut(s) 10, 2402, 4979
FauNDI CATATG 2 cut(s) 1053, 3679
FbaI TGATCA 5 cut(s) 820, 3028, 4581, 5067, 5487
FblI GTMKAC 1 cut(s) 1733
FokI GGATG 9 cut(s) 716, 851, 3028, 3154, 3523, 4245, 4300, 5620, 5833
FriOI GRGCYC 3 cut(s) 2176, 5001, 5337
FspI TGCGCA 1 cut(s) 2448
GlaI GCGC 4 cut(s) 421, 959, 1800, 2448
GsaI CCCAGC 1 cut(s) 1857
GsuI CTGGAG 3 cut(s) 2951, 3084, 3216
HapII CCGG 7 cut(s) 23, 910, 1285, 3523, 5271, 5482, 5851
HgaI GACGC 4 cut(s) 70, 344, 496, 3652
HhaI GCGC 4 cut(s) 422, 960, 1801, 2449
Hin6I GCGC 4 cut(s) 420, 958, 1799, 2447
HinP1I GCGC 4 cut(s) 420, 958, 1799, 2447
HincII GTYRAC 2 cut(s) 1408, 2779
HindII GTYRAC 2 cut(s) 1408, 2779
HindIII AAGCTT 8 cut(s) 387, 869, 1447, 1560, 1595, 3528, 4056, 5625
HpaI GTTAAC 1 cut(s) 1408
HpaII CCGG 7 cut(s) 23, 910, 1285, 3523, 5271, 5482, 5851
HphI GGTGA 8 cut(s) 1145, 1830, 2810, 3734, 4277, 4543, 5413, 5710
Hpy99I CGWCG 4 cut(s) 10, 13, 1347, 2265
HpyCH4IV ACGT 8 cut(s) 247, 283, 340, 1179, 1342, 2098, 3162, 5278
HpySE526I ACGT 8 cut(s) 247, 283, 340, 1179, 1342, 2098, 3162, 5278
HspAI GCGC 4 cut(s) 420, 958, 1799, 2447
Kpn2I TCCGGA 1 cut(s) 5270
Ksp22I TGATCA 5 cut(s) 820, 3028, 4581, 5067, 5487
KspAI GTTAAC 1 cut(s) 1408
LguI GCTCTTC 4 cut(s) 279, 651, 2835, 5783
LmnI GCTCC 8 cut(s) 139, 766, 1203, 2179, 3065, 3535, 4135, 5116
MabI ACCWGGT 2 cut(s) 3915, 4626
MaeII ACGT 8 cut(s) 247, 283, 340, 1179, 1342, 2098, 3162, 5278
MbiI CCGCTC 1 cut(s) 17
MfeI CAATTG 4 cut(s) 3882, 4023, 4557, 4814
MflI RGATCY 6 cut(s) 229, 2297, 2488, 2589, 3454, 4907
MhlI GDGCHC 9 cut(s) 771, 798, 850, 1979, 2176, 2830, 3736, 5001, 5337
MlsI TGGCCA 5 cut(s) 152, 2612, 3560, 4226, 5662
MluI ACGCGT 1 cut(s) 4001
MluNI TGGCCA 5 cut(s) 152, 2612, 3560, 4226, 5662
MlyI GAGTC 6 cut(s) 1102, 1159, 3006, 3158, 3487, 5668
MmeI TCCRAC 5 cut(s) 207, 329, 816, 2099, 3195
Mox20I TGGCCA 5 cut(s) 152, 2612, 3560, 4226, 5662
Mph1103I ATGCAT 2 cut(s) 1971, 2676
MroI TCCGGA 1 cut(s) 5270
MroXI GAANNNNTTC 4 cut(s) 872, 3456, 4156, 5259
MscI TGGCCA 5 cut(s) 152, 2612, 3560, 4226, 5662
MslI CAYNNNNRTG 6 cut(s) 3161, 3680, 4263, 4403, 4869, 4893
Msp20I TGGCCA 5 cut(s) 152, 2612, 3560, 4226, 5662
MspA1I CMGCKG 5 cut(s) 46, 58, 71, 1694, 2348
MspCI CTTAAG 1 cut(s) 4091
MspI CCGG 7 cut(s) 23, 910, 1285, 3523, 5271, 5482, 5851
MunI CAATTG 4 cut(s) 3882, 4023, 4557, 4814
MvnI CGCG 2 cut(s) 420, 4003
NciI CCSGG 1 cut(s) 5851
NcoI CCATGG 1 cut(s) 3156
NdeI CATATG 2 cut(s) 1053, 3679
NheI GCTAGC 1 cut(s) 2582
NmuCI GTSAC 9 cut(s) 332, 1162, 1836, 1935, 2023, 2798, 2938, 4201, 5527
NsbI TGCGCA 1 cut(s) 2448
NsiI ATGCAT 2 cut(s) 1971, 2676
NspI RCATGY 4 cut(s) 1462, 2674, 2929, 3092
OliI CACNNNNGTG 1 cut(s) 3680
PagI TCATGA 4 cut(s) 526, 2088, 3753, 5424
PciI ACATGT 1 cut(s) 3088
PciSI GCTCTTC 4 cut(s) 279, 651, 2835, 5783
PcsI WCGNNNNNNNCGW 2 cut(s) 253, 5839
PdmI GAANNNNTTC 4 cut(s) 872, 3456, 4156, 5259
PflMI CCANNNNNTGG 2 cut(s) 1127, 1382
PfoI TCCNGGA 3 cut(s) 2919, 3940, 5849
PleI GAGTC 6 cut(s) 1101, 1159, 3005, 3157, 3487, 5668
PmaCI CACGTG 1 cut(s) 341
PmlI CACGTG 1 cut(s) 341
PpsI GAGTC 6 cut(s) 1101, 1159, 3005, 3157, 3487, 5668
Ppu21I YACGTR 2 cut(s) 341, 2099
PpuMI RGGWCCY 1 cut(s) 3944
PscI ACATGT 1 cut(s) 3088
PshBI ATTAAT 1 cut(s) 5498
PsiI TTATAA 3 cut(s) 1577, 2495, 2756
Psp124BI GAGCTC 1 cut(s) 5001
Psp5II RGGWCCY 1 cut(s) 3944
PspCI CACGTG 1 cut(s) 341
PspEI GGTNACC 3 cut(s) 927, 2798, 5095
PspFI CCCAGC 1 cut(s) 1853
PspPI GGNCC 9 cut(s) 20, 32, 907, 1519, 2072, 2551, 2962, 3944, 4825
PspPPI RGGWCCY 1 cut(s) 3944
PstI CTGCAG 2 cut(s) 723, 5562
PstNI CAGNNNCTG 4 cut(s) 1313, 1697, 3662, 3947
PsuI RGATCY 6 cut(s) 229, 2297, 2488, 2589, 3454, 4907
PvuII CAGCTG 2 cut(s) 71, 1694
RsaI GTAC 9 cut(s) 1997, 2097, 3499, 3518, 3903, 4576, 5021, 5310, 5468
RsaNI GTAC 9 cut(s) 1996, 2096, 3498, 3517, 3902, 4575, 5020, 5309, 5467
RseI CAYNNNNRTG 6 cut(s) 3161, 3680, 4263, 4403, 4869, 4893
SacI GAGCTC 1 cut(s) 5001
SapI GCTCTTC 4 cut(s) 279, 651, 2835, 5783
Sau96I GGNCC 9 cut(s) 20, 32, 907, 1519, 2072, 2551, 2962, 3944, 4825
SbfI CCTGCAGG 1 cut(s) 5562
ScaI AGTACT 1 cut(s) 4576
SchI GAGTC 6 cut(s) 1102, 1159, 3006, 3158, 3487, 5668
SdaI CCTGCAGG 1 cut(s) 5562
SduI GDGCHC 9 cut(s) 771, 798, 850, 1979, 2176, 2830, 3736, 5001, 5337
SexAI ACCWGGT 2 cut(s) 3915, 4626
SfcI CTRYAG 5 cut(s) 719, 1906, 4911, 4957, 5558
SinI GGWCC 4 cut(s) 2072, 2551, 2962, 3944
SmiMI CAYNNNNRTG 6 cut(s) 3161, 3680, 4263, 4403, 4869, 4893
SmlI CTYRAG 4 cut(s) 1892, 3395, 4091, 4662
SmoI CTYRAG 4 cut(s) 1892, 3395, 4091, 4662
SnaBI TACGTA 1 cut(s) 2099
Sse8387I CCTGCAGG 1 cut(s) 5562
SspI AATATT 4 cut(s) 1536, 1590, 2235, 5502
SstI GAGCTC 1 cut(s) 5001
StyI CCWWGG 6 cut(s) 432, 668, 2266, 2613, 3156, 4317
TaiI ACGT 8 cut(s) 250, 286, 343, 1182, 1345, 2101, 3165, 5281
TaqII GACCGA 1 cut(s) 4415
TatI WGTACW 4 cut(s) 1995, 3516, 3901, 4574
TauI GCSGC 6 cut(s) 37, 40, 43, 49, 92, 404
TscAI CASTG 9 cut(s) 106, 1238, 1278, 2120, 2567, 4591, 4602, 5077, 5464
TseFI GTSAC 9 cut(s) 332, 1162, 1836, 1935, 2023, 2798, 2938, 4201, 5527
Tsp45I GTSAC 9 cut(s) 332, 1162, 1836, 1935, 2023, 2798, 2938, 4201, 5527
TspGWI ACGGA 8 cut(s) 2249, 2293, 2798, 3009, 4358, 4488, 4520, 4891
TspRI CASTG 9 cut(s) 106, 1238, 1278, 2120, 2567, 4591, 4602, 5077, 5464
Van91I CCANNNNNTGG 2 cut(s) 1127, 1382
Vha464I CTTAAG 1 cut(s) 4091
VneI GTGCAC 2 cut(s) 794, 3732
VpaK11BI GGWCC 4 cut(s) 2072, 2551, 2962, 3944
VspI ATTAAT 1 cut(s) 5498
XagI CCTNNNNNAGG 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 1612
XceI RCATGY 4 cut(s) 1462, 2674, 2929, 3092
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 3313, 5374
XmiI GTMKAC 1 cut(s) 1733
XmnI GAANNNNTTC 4 cut(s) 872, 3456, 4156, 5259
ZrmI AGTACT 1 cut(s) 4576
Zsp2I ATGCAT 2 cut(s) 1971, 2676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.