Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0005959

Physical Location & Seq

Genomic Coordinates Reverse (-)

979573 .. 988771

Gene Structure 9199 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g01250.8

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2613 bp

ATGGCGTCGTCGTCGAGAGGGGGTGGTTCGGAACAGCCACCTCAGCAGGCACTGCAGAGGCGGATAACCCGAACCCAGACCGCCGGGAACCTTGGGGAGACGGCATTCGACAGTGAGGTCGTGCCCTCTTCCCTTGTCGAAATCGCTCCCATTCTTCGTGTTGCCAATGAAATCGAATCGCACAATCCTAGAGTTGCTTATCTCTGTCGATTTTATGCCTTTGAGAAGGCTCATAGGCTGGATCCCACTTCTAGTGGACGTGGCGTTCGTCAGTTTAAAACTGCCCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACACTCAAGGGAAGGGTTAAAAAAAGTGATGCACGTGAAATGCAGAGCTTCTACCATCATTACTACAAAAAATATATTCAAGCTTTGTCAAGTACTCATAAAGCTGACCGTGCACAGCTTACCAAGGCATACCAGACTGCCAATGTTCTCTTTGAGGTTTTAAAGGCTGTTACAATGACACAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGATAAAGTTACAGAAAAGACACAGCTTTTGGTCCCTTACAATATCCTTCCACTTGATCCTGATAGTGCAAATCAAGCAATTATGAAATATCCAGAGGTACAAGCTGCTGTTTTTGCTCTTCGTAACACCAGGGGTCTTCCTCGGCCAAAGGATGACAATAAGAAAAACGAGGAAGACATTCTGGATTGGCTTCAGTCAATGTTTGGGTTTCAGAAGGATAATGTGGCAAATCAGAGGGAGCACCTTATACTTTTGCTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAACCTAAGTTGGATGATCGTGCATTGACAGAAGTGATGAAGAAGCTTTTCAAGAACTACAAAAAATGGTGCAAGTATTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACAATACAGCAAGAGGTGCAACAGCGCAAACTACTATACATGGGTCTATATCTTCTGATATGGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAGTGCCTTTGTTATATCTATCACCATATGGCATTTGAATTGTATGGTATGCTCGCTGGGAATGTCAGTCCAATGACGGGAGAGAATGTGAAGCCAGCCTATGGTGGCGAAGAAGAGGCTTTCTTGAGAAAAGTTGTGACTCCAATTTATGAAGTGATTGCAAAAGAGGCTGAAAGGAGTAAACGAGGGAAATCAAAGCATTCCCAGTGGAGAAATTATGATGATTTAAATGAGTACTTTTGGTCAGTGGATTGTTTCCGGTTAGGCTGGCCAATGCGTGCAGGTGCTGATTTCTTTTGCATGCCTATTGATCAGCGTCATTTTGATAAAATTAATGAGGATAAAAAGCCAGCTAGTGGAGATCGATGGGTGGGGAAAGTTAATTTCGTTGAGATACGATCATTTTGGCATATTTTTAGAAGCTTTGACCGCATGTGGAGCTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAACGGCTCGGGGCAACCTACTGCATTATTTAATGGTGATGTATTCACGAAAGCATTGTCTGTCTTCATAACTGCTGCTGTATTGAAACTTGGGCAAGCGTTTCTAGATGTAATTCTTAGCTGGAAGGGTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATACATTTTGAAGGTCATCACAGCTGCTGGGTGGGTGGTAATTCTACCGATTACTTATGCATATAGTTGGAAAAACCCTCCTGCGTTTGCTCAAACTATCAAAAGTTGGTTTGGCAATGGTGGACATCAACCTTCTTTGTTTATCTTGGCTGTTGTTATCTACTTGTCACCAAACATGCTGGCTGCGGTGCTGTTTCTGTTCCCATTTATTCGTAGATTCCTTGATCGATCAAATTATAAGATTGTGACGTTTATGATGTGGTGGTCACAGCCTCGACTTTATGTCGGGAGGGGAATGCATGAGAGCACATTTTCCCTTTTCAAGTACACAATGTTTTGGGTACTTCTCATAATTACCAAGCTGGCATTCAGTTACTATATAGAGATAAAACCTTTAGTTGGTCCAACAAAAGCCATCATGAGTGTACGTGTAACTAATTTTCAGTGGCACGAGTTTTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTTGTGTATTTTATGGATACCCAGATTTGGTATGCCATTTTCTCCACAATATTTGGAGGAATTTATGGTGCATTCCGTCGTCTTGGAGAGATTCGAACATTAGGAATGCTGAGATCTCGTTTTCAATCACTTCCTGGTGCTTTTAATGCCCGTTTGATTCCGGCGGAAAAGAGTCAACCAAGGAAGAAAGGACTGAAGGCTACTTTGTCTCGCAACTTTGCTCAGGTTGAAGTCAACAAAGAGAAAGAGGCAGCAAGATTTGCTCAATTATGGAACAAGATAATAAGTAGTTTCCGAGAGGAGGATCTTATAAACAATAGGGAGATGAACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGATTTAGGCCCCCTTACTCAGTGGCCTCCATTTTTACTTGCCAGCAAGATCGACCGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

871

Amino Acids

100.85

Weight (kDa)

9.71

Isoelectric Point (pI)

45.48

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G13000 AT5G13000
fragaria_vesca	FvH4_3g44290 FvH4_3g44290
malus_domestica	MD03G1015000.v1.1 MD11G1016200.v1.1
prunus_persica	Prupe.6G014400_v2.0.a1 Prupe.6G014400_v2.0.a1 Prupe.6G014400_v2.0.a1 Prupe.6G014400_v2.0.a1 Prupe.6G014400_v2.0.a1
pyrus_communis	pycom03g01250 pycom11g01140
rosa_chinensis	RchiOBHm_Chr5g0080661
rosa_laevigata	RLG00000036880
rosa_multiflora	Rmu_sc0002380.1_g000009 Rmu_sc0029489.1_g000001
rosa_roxburghii	Rroxscaffold_1G00002740
rosa_rugosa	Rorug05G0471000 Rorug05G0471100.1 Rorug05G0471200 Rorug05G0471300
rosa_samantha	Rh5AG524300 Rh5BG546600 Rh5CG569800 Rh5DG556800
rosa_wichuraiana	Rw5G048640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1917, 2504
AarI	CACCTGC	1 cut(s)	1307
AasI	GACNNNNNNGTC	1 cut(s)	116
Acc36I	ACCTGC	1 cut(s)	1307
AccB7I	CCANNNNNTGG	2 cut(s)	1136, 1391
AciI	CCGC	6 cut(s)	61, 81, 1465, 1651, 1865, 2357
AclWI	GGATC	5 cut(s)	236, 249, 308, 587, 2505
AcoI	YGGCCR	2 cut(s)	680, 1304
AcsI	RAATTY	2 cut(s)	1018, 2253
AcuI	CTGAAG	2 cut(s)	713, 2408
AcvI	CACGTG	1 cut(s)	353
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	6 cut(s)	412, 636, 1271, 2006, 2022, 2106
AfiI	CCNNNNNNNGG	6 cut(s)	1112, 1136, 1391, 2078, 2220, 2494
AflIII	ACRYGT	1 cut(s)	2107
AhdI	GACNNNNNGTC	1 cut(s)	1961
AjiI	CACGTC	1 cut(s)	260
AjnI	CCWGG	2 cut(s)	665, 2326
AjuI	GAANNNNNNNTTGG	2 cut(s)	2030, 2062
AloI	GAACNNNNNNTCC	2 cut(s)	249, 281
Alw21I	GWGCWC	4 cut(s)	433, 780, 807, 1988
Alw26I	GTCTC	2 cut(s)	92, 2406
Alw44I	GTGCAC	2 cut(s)	429, 803
AlwI	GGATC	5 cut(s)	236, 249, 308, 587, 2505
AlwNI	CAGNNNCTG	3 cut(s)	52, 1322, 1706
Ama87I	CYCGRG	1 cut(s)	1525
AoxI	GGCC	5 cut(s)	680, 916, 1304, 2560, 2576
ApaLI	GTGCAC	2 cut(s)	429, 803
ApeKI	GCWGC	6 cut(s)	641, 1013, 1592, 1703, 1862, 2444
ApoI	RAATTY	2 cut(s)	1018, 2253
ArsI	GACNNNNNNTTYG	6 cut(s)	249, 281, 547, 579, 1559, 1591
AseI	ATTAAT	1 cut(s)	1368
Asp700I	GAANNNNTTC	2 cut(s)	714, 881
AspLEI	GCGC	1 cut(s)	969
AspS9I	GGNCC	4 cut(s)	568, 916, 2081, 2561
AsuC2I	CCSGG	1 cut(s)	85
AsuHPI	GGTGA	3 cut(s)	1049, 1565, 1839
AsuII	TTCGAA	1 cut(s)	2287
AvaI	CYCGRG	1 cut(s)	1525
AvaII	GGWCC	2 cut(s)	568, 2081
BaeGI	GKGCMC	3 cut(s)	126, 433, 807
BalI	TGGCCA	1 cut(s)	1306
BamHI	GGATCC	1 cut(s)	241
BanII	GRGCYC	1 cut(s)	2185
BauI	CACGAG	1 cut(s)	2129
BbrPI	CACGTG	1 cut(s)	353
BbsI	GAAGAC	3 cut(s)	665, 717, 1573
Bbv12I	GWGCWC	4 cut(s)	433, 780, 807, 1988
BbvCI	CCTCAGC	1 cut(s)	42
BbvI	GCAGC	6 cut(s)	628, 1000, 1579, 1690, 1849, 2456
BccI	CCATC	3 cut(s)	381, 1395, 2102
BceAI	ACGGC	2 cut(s)	117, 1537
BciT130I	CCWGG	2 cut(s)	667, 2328
BciVI	GTATCC	1 cut(s)	2203
BclI	TGATCA	2 cut(s)	829, 1345
BcnI	CCSGG	1 cut(s)	85
BcoDI	GTCTC	2 cut(s)	92, 2406
BfaI	CTAG	4 cut(s)	189, 252, 1389, 1622
BfmI	CTRYAG	1 cut(s)	53
BfuAI	ACCTGC	1 cut(s)	1307
BfuI	GTATCC	1 cut(s)	2203
BglII	AGATCT	1 cut(s)	2306
BisI	GCNGC	6 cut(s)	642, 1014, 1593, 1704, 1863, 2445
BlsI	GCNGC	6 cut(s)	643, 1015, 1594, 1705, 1864, 2446
BmcAI	AGTACT	2 cut(s)	412, 1271
Bme1390I	CCNGG	3 cut(s)	85, 667, 2328
Bme18I	GGWCC	2 cut(s)	568, 2081
BmeRI	GACNNNNNGTC	1 cut(s)	1961
BmeT110I	CYCGRG	1 cut(s)	1525
BmgBI	CACGTC	1 cut(s)	260
BmgT120I	GGNCC	4 cut(s)	568, 916, 2081, 2561
BmiI	GGNNCC	5 cut(s)	89, 243, 570, 2184, 2563
BmrFI	CCNGG	3 cut(s)	85, 667, 2328
BmrI	ACTGGG	1 cut(s)	1234
BmsI	GCATC	1 cut(s)	337
BmuI	ACTGGG	1 cut(s)	1234
BpiI	GAAGAC	3 cut(s)	665, 717, 1573
Bpu10I	CCTNAGC	2 cut(s)	42, 2415
Bpu14I	TTCGAA	1 cut(s)	2287
BpuEI	CTTGAG	2 cut(s)	308, 1180
BpuMI	CCSGG	1 cut(s)	85
Bsa29I	ATCGAT	2 cut(s)	1399, 1906
BsaAI	YACGTR	2 cut(s)	353, 2108
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	5 cut(s)	91, 441, 666, 677, 2372
BsaWI	WCCGGW	1 cut(s)	1293
BsaXI	ACNNNNNCTCC	2 cut(s)	1107, 1137
Bsc4I	CCNNNNNNNGG	6 cut(s)	1112, 1136, 1391, 2078, 2220, 2494
Bse1I	ACTGG	1 cut(s)	1240
Bse3DI	GCAATG	1 cut(s)	1801
BseBI	CCWGG	2 cut(s)	667, 2328
BseCI	ATCGAT	2 cut(s)	1399, 1906
BseDI	CCNNGG	5 cut(s)	91, 441, 666, 677, 2372
BseGI	GGATG	2 cut(s)	694, 853
BseLI	CCNNNNNNNGG	6 cut(s)	1112, 1136, 1391, 2078, 2220, 2494
BseMI	GCAATG	1 cut(s)	1801
BseMII	CTCAG	4 cut(s)	56, 2294, 2429, 2585
BseNI	ACTGG	1 cut(s)	1240
BseRI	GAGGAG	1 cut(s)	2507
BseSI	GKGCMC	3 cut(s)	126, 433, 807
BseXI	GCAGC	6 cut(s)	628, 1000, 1579, 1690, 1849, 2456
BseYI	CCCAGC	2 cut(s)	1091, 1706
BsgI	GTGCAG	1 cut(s)	1335
Bsh1285I	CGRYCG	1 cut(s)	2608
BshFI	GGCC	5 cut(s)	682, 918, 1306, 2562, 2578
BshVI	ATCGAT	2 cut(s)	1399, 1906
BsiEI	CGRYCG	1 cut(s)	2608
BsiHKAI	GWGCWC	4 cut(s)	433, 780, 807, 1988
BsiHKCI	CYCGRG	1 cut(s)	1525
BsiSI	CCGG	4 cut(s)	84, 919, 1294, 2354
BslFI	GGGAC	1 cut(s)	554
BslI	CCNNNNNNNGG	6 cut(s)	1112, 1136, 1391, 2078, 2220, 2494
BsmAI	GTCTC	2 cut(s)	92, 2406
BsmBI	CGTCTC	1 cut(s)	92
BsmFI	GGGAC	1 cut(s)	554
BsmI	GAATGC	6 cut(s)	104, 1234, 1980, 2045, 2264, 2304
BsnI	GGCC	5 cut(s)	682, 918, 1306, 2562, 2578
BsoBI	CYCGRG	1 cut(s)	1525
Bsp119I	TTCGAA	1 cut(s)	2287
Bsp1286I	GDGCHC	6 cut(s)	126, 433, 780, 807, 1988, 2185
BspACI	CCGC	6 cut(s)	61, 81, 1465, 1651, 1865, 2357
BspANI	GGCC	5 cut(s)	682, 918, 1306, 2562, 2578
BspCNI	CTCAG	4 cut(s)	55, 2295, 2428, 2584
BspDI	ATCGAT	2 cut(s)	1399, 1906
BspHI	TCATGA	2 cut(s)	535, 2097
BspLI	GGNNCC	5 cut(s)	89, 243, 570, 2184, 2563
BspMAI	CTGCAG	1 cut(s)	57
BspMI	ACCTGC	1 cut(s)	1307
BspPI	GGATC	5 cut(s)	236, 249, 308, 587, 2505
BspQI	GCTCTTC	1 cut(s)	660
BspT104I	TTCGAA	1 cut(s)	2287
BsrDI	GCAATG	1 cut(s)	1801
BsrI	ACTGG	1 cut(s)	1240
BssECI	CCNNGG	5 cut(s)	91, 441, 666, 677, 2372
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	2129
BssT1I	CCWWGG	3 cut(s)	91, 441, 2372
Bst2BI	CACGAG	1 cut(s)	2129
Bst2UI	CCWGG	2 cut(s)	667, 2328
Bst4CI	ACNGT	2 cut(s)	113, 428
Bst6I	CTCTTC	3 cut(s)	133, 660, 1143
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	3 cut(s)	52, 958, 2453
BstBAI	YACGTR	2 cut(s)	353, 2108
BstBI	TTCGAA	1 cut(s)	2287
BstDEI	CTNAG	6 cut(s)	42, 840, 1634, 2303, 2415, 2571
BstEII	GGTNACC	1 cut(s)	936
BstF5I	GGATG	2 cut(s)	694, 853
BstHHI	GCGC	1 cut(s)	969
BstMAI	GTCTC	2 cut(s)	92, 2406
BstMCI	CGRYCG	1 cut(s)	2608
BstNI	CCWGG	2 cut(s)	667, 2328
BstNSI	RCATGY	3 cut(s)	1339, 1471, 1858
BstPI	GGTNACC	1 cut(s)	936
BstSCI	CCNGG	3 cut(s)	83, 665, 2326
BstSFI	CTRYAG	1 cut(s)	53
BstSLI	GKGCMC	3 cut(s)	126, 433, 807
BstV1I	GCAGC	6 cut(s)	628, 1000, 1579, 1690, 1849, 2456
BstV2I	GAAGAC	3 cut(s)	665, 717, 1573
BstX2I	RGATCY	3 cut(s)	241, 2306, 2497
BstYI	RGATCY	3 cut(s)	241, 2306, 2497
Bsu15I	ATCGAT	2 cut(s)	1399, 1906
BsuI	GTATCC	1 cut(s)	2203
BsuRI	GGCC	5 cut(s)	682, 918, 1306, 2562, 2578
BsuTUI	ATCGAT	2 cut(s)	1399, 1906
BtrI	CACGTC	1 cut(s)	260
BtsCI	GGATG	2 cut(s)	694, 853
BtsI	GCAGTG	1 cut(s)	50
BtsIMutI	CAGTG	6 cut(s)	50, 118, 1247, 1287, 2129, 2579
BveI	ACCTGC	1 cut(s)	1307
CaiI	CAGNNNCTG	3 cut(s)	52, 1322, 1706
CciI	TCATGA	2 cut(s)	535, 2097
CfoI	GCGC	1 cut(s)	969
Cfr13I	GGNCC	4 cut(s)	568, 916, 2081, 2561
ClaI	ATCGAT	2 cut(s)	1399, 1906
CseI	GACGC	1 cut(s)	1340
Csp6I	GTAC	6 cut(s)	411, 635, 1270, 2005, 2021, 2105
CspCI	CAANNNNNGTGG	2 cut(s)	2227, 2262
CviAII	CATG	9 cut(s)	536, 982, 1030, 1336, 1468, 1667, 1855, 1979, 2098
CviQI	GTAC	6 cut(s)	411, 635, 1270, 2005, 2021, 2105
DdeI	CTNAG	6 cut(s)	42, 840, 1634, 2303, 2415, 2571
DraI	TTTAAA	3 cut(s)	277, 480, 1263
DrdI	GACNNNNNNGTC	1 cut(s)	116
DriI	GACNNNNNGTC	1 cut(s)	1961
DseDI	GACNNNNNNGTC	1 cut(s)	116
EaeI	YGGCCR	2 cut(s)	680, 1304
Eam1104I	CTCTTC	3 cut(s)	133, 660, 1143
Eam1105I	GACNNNNNGTC	1 cut(s)	1961
EarI	CTCTTC	3 cut(s)	133, 660, 1143
EciI	GGCGGA	3 cut(s)	76, 1666, 2372
Eco130I	CCWWGG	3 cut(s)	91, 441, 2372
Eco24I	GRGCYC	1 cut(s)	2185
Eco47I	GGWCC	2 cut(s)	568, 2081
Eco57I	CTGAAG	2 cut(s)	713, 2408
Eco72I	CACGTG	1 cut(s)	353
Eco88I	CYCGRG	1 cut(s)	1525
Eco91I	GGTNACC	1 cut(s)	936
EcoO109I	RGGNCCY	1 cut(s)	2561
EcoO65I	GGTNACC	1 cut(s)	936
EcoRII	CCWGG	2 cut(s)	665, 2326
EcoT14I	CCWWGG	3 cut(s)	91, 441, 2372
EcoT22I	ATGCAT	2 cut(s)	1741, 1980
EcoT38I	GRGCYC	1 cut(s)	2185
ErhI	CCWWGG	3 cut(s)	91, 441, 2372
Esp3I	CGTCTC	1 cut(s)	92
FaeI	CATG	9 cut(s)	539, 985, 1033, 1339, 1471, 1670, 1858, 1982, 2101
FaqI	GGGAC	1 cut(s)	554
FatI	CATG	9 cut(s)	535, 981, 1029, 1335, 1467, 1666, 1854, 1978, 2097
FauNDI	CATATG	1 cut(s)	1062
FbaI	TGATCA	2 cut(s)	829, 1345
Fnu4HI	GCNGC	6 cut(s)	642, 1014, 1593, 1704, 1863, 2445
FokI	GGATG	2 cut(s)	701, 860
FriOI	GRGCYC	1 cut(s)	2185
Fsp4HI	GCNGC	6 cut(s)	642, 1014, 1593, 1704, 1863, 2445
FspBI	CTAG	4 cut(s)	189, 252, 1389, 1622
GlaI	GCGC	1 cut(s)	968
GluI	GCNGC	6 cut(s)	642, 1014, 1593, 1704, 1863, 2445
GsaI	CCCAGC	2 cut(s)	1095, 1710
HaeIII	GGCC	5 cut(s)	682, 918, 1306, 2562, 2578
HapII	CCGG	4 cut(s)	84, 919, 1294, 2354
HgaI	GACGC	1 cut(s)	1340
HhaI	GCGC	1 cut(s)	969
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	9 cut(s)	539, 985, 1033, 1339, 1471, 1670, 1858, 1982, 2101
Hin6I	GCGC	1 cut(s)	967
HinP1I	GCGC	1 cut(s)	967
HincII	GTYRAC	2 cut(s)	2369, 2428
HindII	GTYRAC	2 cut(s)	2369, 2428
HindIII	AAGCTT	3 cut(s)	399, 878, 1456
HinfI	GANTC	7 cut(s)	176, 1026, 1174, 1896, 2284, 2350, 2365
HpaII	CCGG	4 cut(s)	84, 919, 1294, 2354
HphI	GGTGA	3 cut(s)	1049, 1565, 1839
Hpy188I	TCNGA	5 cut(s)	31, 750, 771, 999, 2489
Hpy99I	CGWCG	4 cut(s)	10, 13, 16, 2274
HpyAV	CCTTC	9 cut(s)	220, 296, 324, 593, 745, 1636, 1684, 1821, 2383
HpyCH4III	ACNGT	2 cut(s)	113, 428
HpyCH4IV	ACGT	5 cut(s)	259, 295, 352, 1928, 2107
HpyF3I	CTNAG	6 cut(s)	42, 840, 1634, 2303, 2415, 2571
HpySE526I	ACGT	5 cut(s)	259, 295, 352, 1928, 2107
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	9 cut(s)	539, 985, 1033, 1339, 1471, 1670, 1858, 1982, 2101
HspAI	GCGC	1 cut(s)	967
Ksp22I	TGATCA	2 cut(s)	829, 1345
LguI	GCTCTTC	1 cut(s)	660
LmnI	GCTCC	4 cut(s)	151, 775, 1473, 2188
Lsp1109I	GCAGC	6 cut(s)	628, 1000, 1579, 1690, 1849, 2456
LweI	GCATC	1 cut(s)	337
MaeI	CTAG	4 cut(s)	189, 252, 1389, 1622
MaeII	ACGT	5 cut(s)	259, 295, 352, 1928, 2107
MflI	RGATCY	3 cut(s)	241, 2306, 2497
MhlI	GDGCHC	6 cut(s)	126, 433, 780, 807, 1988, 2185
MlsI	TGGCCA	1 cut(s)	1306
MluNI	TGGCCA	1 cut(s)	1306
MlyI	GAGTC	2 cut(s)	1168, 2374
MmeI	TCCRAC	4 cut(s)	341, 825, 1727, 2108
Mox20I	TGGCCA	1 cut(s)	1306
Mph1103I	ATGCAT	2 cut(s)	1741, 1980
MroXI	GAANNNNTTC	2 cut(s)	714, 881
MscI	TGGCCA	1 cut(s)	1306
MseI	TTAA	9 cut(s)	276, 336, 479, 1262, 1368, 1416, 1548, 1671, 2337
Msp20I	TGGCCA	1 cut(s)	1306
MspA1I	CMGCKG	1 cut(s)	1703
MspI	CCGG	4 cut(s)	84, 919, 1294, 2354
MspR9I	CCNGG	3 cut(s)	85, 667, 2328
Mva1269I	GAATGC	6 cut(s)	104, 1234, 1980, 2045, 2264, 2304
MvaI	CCWGG	2 cut(s)	667, 2328
NciI	CCSGG	1 cut(s)	85
NdeI	CATATG	1 cut(s)	1062
NlaIII	CATG	9 cut(s)	539, 985, 1033, 1339, 1471, 1670, 1858, 1982, 2101
NlaIV	GGNNCC	5 cut(s)	89, 243, 570, 2184, 2563
NmeAIII	GCCGAG	1 cut(s)	658
NmuCI	GTSAC	4 cut(s)	1171, 1845, 1924, 1944
NsiI	ATGCAT	2 cut(s)	1741, 1980
NspI	RCATGY	3 cut(s)	1339, 1471, 1858
NspV	TTCGAA	1 cut(s)	2287
PaeI	GCATGC	1 cut(s)	1339
PagI	TCATGA	2 cut(s)	535, 2097
PaqCI	CACCTGC	1 cut(s)	1307
PciSI	GCTCTTC	1 cut(s)	660
PcsI	WCGNNNNNNNCGW	1 cut(s)	265
PctI	GAATGC	6 cut(s)	104, 1234, 1980, 2045, 2264, 2304
PdmI	GAANNNNTTC	2 cut(s)	714, 881
PfeI	GAWTC	5 cut(s)	176, 1026, 1896, 2284, 2350
PflMI	CCANNNNNTGG	2 cut(s)	1136, 1391
PkrI	GCNGC	6 cut(s)	643, 1015, 1594, 1705, 1864, 2446
PleI	GAGTC	2 cut(s)	1168, 2373
PmaCI	CACGTG	1 cut(s)	353
PmlI	CACGTG	1 cut(s)	353
PpsI	GAGTC	2 cut(s)	1168, 2373
Ppu21I	YACGTR	2 cut(s)	353, 2108
PshBI	ATTAAT	1 cut(s)	1368
PsiI	TTATAA	2 cut(s)	1917, 2504
Psp6I	CCWGG	2 cut(s)	665, 2326
PspCI	CACGTG	1 cut(s)	353
PspEI	GGTNACC	1 cut(s)	936
PspFI	CCCAGC	2 cut(s)	1091, 1706
PspGI	CCWGG	2 cut(s)	665, 2326
PspN4I	GGNNCC	5 cut(s)	89, 243, 570, 2184, 2563
PspPI	GGNCC	4 cut(s)	568, 916, 2081, 2561
PstI	CTGCAG	1 cut(s)	57
PstNI	CAGNNNCTG	3 cut(s)	52, 1322, 1706
PsuI	RGATCY	3 cut(s)	241, 2306, 2497
PvuII	CAGCTG	1 cut(s)	1703
RsaI	GTAC	6 cut(s)	412, 636, 1271, 2006, 2022, 2106
RsaNI	GTAC	6 cut(s)	411, 635, 1270, 2005, 2021, 2105
SapI	GCTCTTC	1 cut(s)	660
SaqAI	TTAA	9 cut(s)	276, 336, 479, 1262, 1368, 1416, 1548, 1671, 2337
SatI	GCNGC	6 cut(s)	642, 1014, 1593, 1704, 1863, 2445
Sau96I	GGNCC	4 cut(s)	568, 916, 2081, 2561
ScaI	AGTACT	2 cut(s)	412, 1271
SchI	GAGTC	2 cut(s)	1168, 2374
ScrFI	CCNGG	3 cut(s)	85, 667, 2328
SduI	GDGCHC	6 cut(s)	126, 433, 780, 807, 1988, 2185
SfaNI	GCATC	1 cut(s)	337
SfcI	CTRYAG	1 cut(s)	53
SfuI	TTCGAA	1 cut(s)	2287
SinI	GGWCC	2 cut(s)	568, 2081
SmiI	ATTTAAAT	1 cut(s)	1263
SmlI	CTYRAG	2 cut(s)	323, 1159
SmoI	CTYRAG	2 cut(s)	323, 1159
SphI	GCATGC	1 cut(s)	1339
SsiI	CCGC	6 cut(s)	61, 81, 1465, 1651, 1865, 2357
SspI	AATATT	1 cut(s)	2244
SspMI	CTAG	4 cut(s)	189, 252, 1389, 1622
StyD4I	CCNGG	3 cut(s)	83, 665, 2326
StyI	CCWWGG	3 cut(s)	91, 441, 2372
SwaI	ATTTAAAT	1 cut(s)	1263
TaaI	ACNGT	2 cut(s)	113, 428
TaiI	ACGT	5 cut(s)	262, 298, 355, 1931, 2110
TatI	WGTACW	3 cut(s)	410, 1269, 2004
TfiI	GAWTC	5 cut(s)	176, 1026, 1896, 2284, 2350
Tru1I	TTAA	9 cut(s)	276, 336, 479, 1262, 1368, 1416, 1548, 1671, 2337
Tru9I	TTAA	9 cut(s)	276, 336, 479, 1262, 1368, 1416, 1548, 1671, 2337
TscAI	CASTG	6 cut(s)	57, 118, 1247, 1287, 2129, 2579
TseFI	GTSAC	4 cut(s)	1171, 1845, 1924, 1944
TseI	GCWGC	6 cut(s)	641, 1013, 1592, 1703, 1862, 2444
Tsp45I	GTSAC	4 cut(s)	1171, 1845, 1924, 1944
TspDTI	ATGAA	7 cut(s)	183, 635, 887, 1018, 1200, 1573, 2534
TspGWI	ACGGA	1 cut(s)	2258
TspRI	CASTG	6 cut(s)	57, 118, 1247, 1287, 2129, 2579
Van91I	CCANNNNNTGG	2 cut(s)	1136, 1391
VneI	GTGCAC	2 cut(s)	429, 803
VpaK11BI	GGWCC	2 cut(s)	568, 2081
VspI	ATTAAT	1 cut(s)	1368
XapI	RAATTY	2 cut(s)	1018, 2253
XbaI	TCTAGA	1 cut(s)	1621
XceI	RCATGY	3 cut(s)	1339, 1471, 1858
XmnI	GAANNNNTTC	2 cut(s)	714, 881
XspI	CTAG	4 cut(s)	189, 252, 1389, 1622
ZrmI	AGTACT	2 cut(s)	412, 1271
Zsp2I	ATGCAT	2 cut(s)	1741, 1980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.