MD11G1016200.v1.1

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005959
Physical Location & Seq
Reverse (-)
1287011 .. 1303616
16606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1016200.v1.1.491

Sequence Viewer

Length: 5874 bp
ATGGCGTCGTCGTCGAGAGGGGGTGGTTCGGATCAGCCACCTCAGCAGCCACTGCAGAGGCGGTTAACTCGAACACAGACCGCCGGGAACATCGGGGAGACGGCTTTCGACAGTGAGATCGTGCCATCGTCCCTTGTCGAAATCGCCCCCATTCTTCGTGTTGCCAATGAAGTCGAAACCAACAATCCTAGAGTTGCTTATCTCTGTCGATTTTATGCCTTCGAGAAGGCTCATAGGCTGGATCCTACTTCTAGTGGACGTGGCGTTCGTCAGTTTAAAACTGCCCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACACTCATGGGAAGGGTTAAAAAAAGTGATGCACGTGAAATGCAGAGCTTCTACCATCATTACTACAAAAAATATATTCAAGCTTTGTCAAATACTCATAAAGCTGACCGTGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTCTTTGAGGTTTTAAAGGCTGTTAATATGACACAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGATAAAGTTGCAGAAAAGACACAGCTCTTGGTCCCTTACAATATCCTTCCACTTGATCCTGATAGTGCAAATCAAGCAATTATGAAATATCCAGAGGTACAAGCTGCTGTTTTTGCCCTTCGTAACACCAGGGGTCTTCCTTGGCCAAAGGAATACAAAAAGAAAAATGAAGAAGACATTCTGGATTGGCTTCAGTCAATGTTTGGGTTTCAGAAGGATAATGTGGCAAATCAACGGGAGCATCTGATACTATTGCTTGCAAATGTGCATATAAGGCAGTTTCCAAAGCCTGATCAACAACCCAAGTTGGATGACCGTGCATTGACAGAAGTGATGAAGAAGCTTTTCAAGAACTACAAAAAATGGTGCAAGTATTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACAATACAGCAAGAGGTGCAACAGCGCAAACTACTATACATGGGTCTATATCTTCTGATATGGGGAGAAGCTGCAAATTTGAGATTCATGCCAGAGTGCCTTTGCTATATCTATCACCACATGGCATTTGAATTGTATGGTATGCTCGCTGGGAATGTCAGTCCAATGACGGGAGAGAATGTGAAGCCTGCCTATGGAGGCGCAGAAGAGGCTTTCTTGAGAAAAGTTGTGACTCCTATTTATTTAGTGATCGCAAAGGAGGCTGAAAGGAGTAAACGAGGGAAATCAAAGCATTCCCAGTGGAGAAATTATGATGATTTAAATGAATACTTCTGGTCGGTGGATTGTTTCCGGTTAGGCTGGCCGATGCGTGCAGGTGCTGATTTCTTTTGCATGCCTACTGATCAGCGTCATTCTGATATAAGTAATGAGGATAAAAAGCCAGCTAGTGGAGATCGATGGGTGGGGAAGGTTAATTTCGTTGAGATACGGTCCTTTTGGCATATTTTTAGAAGCTTTGACCGCATGTGGAGCTTCTTTATTCTATGTTTACAGGTTATGATCATTGTTGCTTGGAATGGCTCGGGGCAACCTACTGGATTATTTGATGGTGAAGTATTCACGAAAGCATTGTCTGTCTTCATAACTGCTGCAGTATTGAAGCTTGGGCAAGCTTTTCTGGATGTAATTCTTAGCTGGAAGGGTAGGCGGAGTATGTCATTCCATGTGAAGTTGAGATACATTTTGAAGGTCATTACAGCTGCTATGTGGGTGGTAATTCTACCGATTACATATGCATATAGTTGGAAAAACCCTCCTGTGTTTGCTCAAACTATCAAAAGTTGGTTTGGCAACAATGGACATCAACCAACCTTGTTTATCTTGGCTGTTGTTATCTACTTGTCACCAAACATGCTGGCTGCGGTGCTGTTTCTGTTCCCATTTATTCGTAGATTCCTTGAGAGATCAAATTATAAGATTGTGATGTTTATGATGTGGTGGTCACAGCCTCGACTTTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCCCTTTTCAAGTACACAATGTTTTGGGTTCTTCTTATAATTACCAAGCTGGCTTTCAGTTACTATATAGAGATAAAACCTTTAGTTGGTCCAACAAAAGCCATCATGAGTGTACGTATAACCAATTTTCAGTGGCACGAGTTTTTTCCCCGTGCAAAGAACAATATTGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTTGTCTATTTTATGGATACCCAGATTTGGTACGCCATTTTCTCCACAATATTTGGAGGAATTTATGGTGCATTCCGTCGCCTTGGGGAGATTCGAACATTAGGAATGCTGAGATCTCGTTTTCAATCACTTCCTGGTGCTTTTAATGCCCGTTTGATTCCGGCGGAAAAGAGTGAACCAAAGAAGAAAGGATTGAAGGCTACTTTGTCTCGCACCTTTGTTCAGGTTGAAGTCAACAAAGAGAAAGAGGCAGCAAGATTTGCTCAATTATGGAACAAGATAATAAGTAGTTTCCGAGAGGAGGATCTTATAAGCAATAGGGAAATGGACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGATTTAGGCTCCCTTACGCAGTGGCCTCCATTTTTGCTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGTTGAAAAAGAGGATTGAGGCTGACAATTACATGTCTTGTGCTGTTTTGGAATGCTATGCTTCATTTAGGAACATAATTAAGTTCCTGGTTCAGGGAGAACGTGAGAAAGAGGTTATAGATGATATATTTTCTGAGGTTGACAAGCACATAGACGCGGGTGACCTGATGGTCGAATACAATATGAGTGCTCTTCCTAGCCTCTATGGCTATTTCGTTAAGCTCATCAAACATCTGTTGGACAATAATAAGGACGAAAGGGATCAAGTTGTGATTCTGTTCCAGGACATGCTGGAGGTAGTGACAAGAGACATAATGGAGGACCATATGTCCAGTTTGGTAGATTCAAGCCATGGTATGTCTGGCCATGAAGGGATGATGCCCCTTGATCAACCTCAACAGTATCAGTTGTTTGCGTCTTTTGGGGCTATCAGGTTTCCTATTCCACAAGTAACAGAGGCTTGGAGGGAGAAGATTAAACGTCTTTATCTTTTGCTTACAACAAAGGAGTCTGCCATGGATGTACCGTCCAACTTAGAAGCTAGAAGGCGGATCTCTTTTTTCTCAAATTCATTATTTATGAACATGCCTCCAGCACCAAAAGTTCGCAATATGCTCTCTTTCTCTGTTTTAACTCCTTACTACACTGAGGAGGTTCTCTTTTCGTTGCGTGATTTGGAAGTACCAAATGAAGATGGGGTTTCAATTCTCTTCTACTTGCAAAAAATTTTCCCAGATGAGTGGAACAACTTTCTTGAGCGTGTGAATTGCACCAGTGAAGAGGAGCTCAAAGGATCAGACGAATTGGAAGAAGATCTTCGCCTTTGGGCATCATATAGAGGGCAAACTTTGACTCGTACTGTAAGGGGAATGATGTACTACCGAAAGGCTTTGGAGCTTCAGTCTTTTCTAGATATGGCCCAAGATGAAGATTTAATGGAAGGCTATAAGGCCATAGAATTGAATTCTGAAGATCAAAAGGAAGGAAGGTCACTGTGGGCGCAATGTCAAGCAGTAGCTGATTTGAAATTCACATATGTGGTATCATGCCAACTATATGGGATACACAAGCGATCTGGTGATGCTCGTGCGCAAGACATTCTGAGGCTTATGACAACATATCCATCACTACGTGTGGCATATATTGATGAGGTTGAGGAACCTAGCAAAGATAGTTCCAAGAAGATCAACCAAAAAGTTTATTACTCTACTTTAGTCAAGGCTGCTCTGCCTAAGTCAATTGATTCTTCAGAGCCCGTTCAAAATCTGGATCAAGTAATTTATCGCATAAAGCTTCCAGGACCTGCTATTTTGGGTGAAGGAAAACCAGAAAATCAAAATCATGCCATTATTTTCACACGTGGAGAAGGATTGCAAACAATTGATATGAACCAGGATAACTACATGGAAGAAGCCTTGAAAATGAGGAATTTGCTGCAAGAATTTCTCGAGAAGCATGATGGGGTTAGGTATCCTACAATTCTTGGGCTTAGGGAGCATATATTTACTGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAAAACAGTTTTGTCACCATTGGTCAGAGACTGTTGGCCAATCCTCTCAGGGTTCGATTTCATTATGGCCATCCTGATGTGTTTGATAGGCTCTTTCACCTTACCAGAGGGGGTGTCAGTAAAGCATCCAAGGTTATTAATTTGAGTGAAGACATATTTGCCGGCTTTAATTCCACACTCCGTGAAGGCAATGTAACTCATCACGAGTACATACAAGTTGGGAAGGGAAGAGATGTGGGCCTTAACCAGATCTCTATGTTCGAAGCAAAAATAGCTAATGGAAATGGGGAGCAGACACTCAGTCGAGATATATACCGACTTGGACACCGTTTTGACTTTTTCCGAATGATGTCTTGCTATTTCACCACAATTGGTTTCTACTACAGTACTCTGATCACTGTGCTTACCGTGTACGTCTTCCTTTATGGACGCCTTTATCTTGTTCTTAGTGGACTTGAAGAGGGTATGAGCACGGAACGAGCAATTAGAGACAACAAGCCTCTGCAAGTGGCCCTTGCTTCTCAATCATTTGTTCAAATAGGATTTTTGATGGCGTTGCCTATGTTGATGGAAATTGGTTTGGAGAAGGGTTTCCGAACTGCACTAAGTGAATTCGTATTGATGCAATTGCAATTGGCCCCAGTATTTTTCACATTTTCACTTGGAACGAAGACCCACTATTATGGAAGGACATTACTTCATGGTGGTGCGAAATATAGATCCACAGGCCGAGGTTTTGTGGTTTTCCATGCCAAATTTGCTGACAACTATAGGCTCTACTCCCGCAGCCACTTTGTTAAGGGCATTGAGCTCTTAATTTTACTCGTTGTGTACCAGATCTTTGGTCATACTTATAGAAGTGCTGTTGCGTACATTTTGATCACTGTGTCTATGTGGTTTATGGTGGGCACCTGGCTGTTTGCTCCCTTTCTGTTCAATCCATCTGGCTTTGAGTGGCAAAAGATTGTTGATGATTGGACGGATTGGAATAAGTGGATAAGCAACCGTGGGGGTATAGGTGTTCCACCTGAAAAAAGCTGGGAATCATGGTGGGAGGAGGAACAAGAGCATCTCCAATATTCTGGAAAACGCGGTATTGTAGCTGAGATACTGTTATCCTTACGGTTCTTTGTCTATCAGTATGGGCTCGTGTATCACTTGAACATCGCAAAGGAAAACAAGAGTGTTCTGGTCTATGGCATTTCGTGGCTGGTGATCGTCGTTATTTTGTTCGTTATGAAGACTGTATCTGTTGGGAGGCGAAAGTTCAGCGCAGAATTCCAACTTGTGTTCCGGCTGATCAAGGGACTGATATTCATAACTTTTGTGTCCATTTTGGTCACTTTGATTGTGCTGCCTCACATGACCCTGCAGGATATCCTCGTCTGCATTCTTGCTTTCATGCCAACTGGCTGGGGAATGCTTCTGATTGCACAAGCTTGCAAGCCTGTCGTGCACAAGGCTGGTTTATGGCCATCGGTTCGGACTCTTGCTCGCGGCTTTGAGATAGTTATGGGCCTGCTTCTATTCACCCCGGTTGCATTCTTGGCCTGGTTTCCTTTTGTTTCTGAATTCCAGACTCGTATGCTCTTCAACCAAGCATTCAGCAGAGGCTTGCAGATTTCTCGTATTCTTGGCGGGCAACGCAAGGATCGCTCATCCCGGAGCAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1958

Amino Acids

225.95

Weight (kDa)

9.14

Isoelectric Point (pI)

44.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 48 - 174 3.1e-14 Vta1 like
FKS1_dom1 PF14288 324 - 436 2e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 860 - 1051 8.9e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1058 - 1148 1.7e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1151 - 1774 6.5e-210 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1917, 2030, 2504
AarI CACCTGC 1 cut(s) 1307
AasI GACNNNNNNGTC 1 cut(s) 2819
Acc16I TGCGCA 1 cut(s) 3741
Acc36I ACCTGC 2 cut(s) 1307, 3961
AccB1I GGYRCC 1 cut(s) 5105
AccB7I CCANNNNNTGG 1 cut(s) 1391
AccII CGCG 3 cut(s) 2807, 5289, 5694
AcoI YGGCCR 7 cut(s) 680, 1304, 2622, 3013, 4233, 4264, 5669
AcuI CTGAAG 4 cut(s) 713, 3533, 3639, 3882
AcvI CACGTG 2 cut(s) 353, 4010
AcyI GRCGYC 2 cut(s) 5, 4627
AdeI CACNNNGTG 3 cut(s) 3782, 4379, 4805
AfiI CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 2743, 5866
AflIII ACRYGT 3 cut(s) 2682, 3781, 4007
AhdI GACNNNNNGTC 2 cut(s) 2977, 4497
AjiI CACGTC 1 cut(s) 260
AjnI CCWGG 8 cut(s) 665, 2326, 2736, 2931, 3946, 4041, 5108, 5747
AjuI GAANNNNNNNTTGG 2 cut(s) 2030, 2062
AleI CACNNNNGTG 1 cut(s) 3686
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 7 cut(s) 433, 1988, 2842, 3438, 4670, 5010, 5655
Alw26I GTCTC 5 cut(s) 92, 2406, 2952, 4219, 4680
Alw44I GTGCAC 2 cut(s) 429, 5651
AlwNI CAGNNNCTG 5 cut(s) 52, 1322, 3668, 3953, 4228
Ama87I CYCGRG 2 cut(s) 1525, 4097
ApaLI GTGCAC 2 cut(s) 429, 5651
ArsI GACNNNNNNTTYG 4 cut(s) 249, 281, 1559, 1591
AseI ATTAAT 1 cut(s) 4335
Asp700I GAANNNNTTC 5 cut(s) 714, 881, 3465, 4165, 4787
AspLEI GCGC 5 cut(s) 969, 1145, 3652, 3742, 5471
AsuC2I CCSGG 3 cut(s) 85, 5732, 5860
AsuII TTCGAA 2 cut(s) 2287, 4458
AsuNHI GCTAGC 1 cut(s) 2594
AvaI CYCGRG 2 cut(s) 1525, 4097
AvaII GGWCC 6 cut(s) 568, 1434, 2081, 2521, 2971, 3950
BaeGI GKGCMC 3 cut(s) 433, 5108, 5655
BalI TGGCCA 6 cut(s) 682, 2624, 3015, 4235, 4266, 5671
BamHI GGATCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 5105
BanII GRGCYC 5 cut(s) 2185, 3438, 3906, 5010, 5346
BarI GAAGNNNNNNTAC 2 cut(s) 1664, 1696
BauI CACGAG 4 cut(s) 2129, 3735, 4400, 5345
BbrPI CACGTG 2 cut(s) 353, 4010
BbsI GAAGAC 7 cut(s) 665, 717, 1573, 4353, 4606, 4874, 5444
Bbv12I GWGCWC 7 cut(s) 433, 1988, 2842, 3438, 4670, 5010, 5655
BbvCI CCTCAGC 1 cut(s) 42
BceAI ACGGC 1 cut(s) 117
BcgI CGANNNNNNTGC 4 cut(s) 5629, 5663, 5804, 5838
BciT130I CCWGG 8 cut(s) 667, 2328, 2738, 2933, 3948, 4043, 5110, 5749
BciVI GTATCC 3 cut(s) 2203, 3705, 4131
BclI TGATCA 7 cut(s) 829, 1345, 1503, 3037, 4590, 5076, 5496
BcnI CCSGG 3 cut(s) 85, 5732, 5860
BcoDI GTCTC 5 cut(s) 92, 2406, 2952, 4219, 4680
BfaI CTAG 9 cut(s) 189, 252, 1389, 2595, 2847, 3192, 3560, 3813, 4175
BfmI CTRYAG 5 cut(s) 53, 1593, 4579, 4966, 5567
BfuAI ACCTGC 2 cut(s) 1307, 3961
BfuI GTATCC 3 cut(s) 2203, 3705, 4131
BglI GCCNNNNNGGC 1 cut(s) 2856
BglII AGATCT 4 cut(s) 2306, 3463, 4446, 5034
BmcAI AGTACT 1 cut(s) 4585
Bme18I GGWCC 6 cut(s) 568, 1434, 2081, 2521, 2971, 3950
BmeRI GACNNNNNGTC 2 cut(s) 2977, 4497
BmeT110I CYCGRG 2 cut(s) 1525, 4097
BmgBI CACGTC 1 cut(s) 260
BmiI GGNNCC 7 cut(s) 243, 570, 2184, 2563, 3810, 4836, 5107
BmrI ACTGGG 2 cut(s) 1234, 4832
BmsI GCATC 9 cut(s) 337, 787, 1299, 3018, 3488, 3721, 4331, 4809, 5275
BmtI GCTAGC 1 cut(s) 2598
BmuI ACTGGG 2 cut(s) 1234, 4832
BpiI GAAGAC 7 cut(s) 665, 717, 1573, 4353, 4606, 4874, 5444
BpmI CTGGAG 2 cut(s) 2963, 3225
Bpu10I CCTNAGC 2 cut(s) 42, 4139
Bpu14I TTCGAA 2 cut(s) 2287, 4458
BpuEI CTTGAG 3 cut(s) 1180, 1922, 3425
BpuMI CCSGG 3 cut(s) 85, 5732, 5860
Bsa29I ATCGAT 1 cut(s) 1399
BsaAI YACGTR 4 cut(s) 353, 2108, 3782, 4010
BsaHI GRCGYC 2 cut(s) 5, 4627
BsaWI WCCGGW 1 cut(s) 1293
BsaXI ACNNNNNCTCC 4 cut(s) 1107, 1137, 3536, 3566
Bsc4I CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 2743, 5866
Bse118I RCCGGY 1 cut(s) 4358
Bse1I ACTGG 7 cut(s) 1240, 1543, 2982, 3423, 4162, 4838, 5611
Bse3DI GCAATG 3 cut(s) 2641, 3659, 4393
BseBI CCWGG 8 cut(s) 667, 2328, 2738, 2933, 3948, 4043, 5110, 5749
BseCI ATCGAT 1 cut(s) 1399
BseGI GGATG 7 cut(s) 853, 1630, 3030, 3175, 4267, 4322, 5855
BseLI CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 2743, 5866
BseMI GCAATG 3 cut(s) 2641, 3659, 4393
BseMII CTCAG 8 cut(s) 56, 2294, 2775, 3288, 3743, 4258, 4510, 5292
BseNI ACTGG 7 cut(s) 1240, 1543, 2982, 3423, 4162, 4838, 5611
BseRI GAGGAG 4 cut(s) 2507, 3314, 3446, 5267
BseSI GKGCMC 3 cut(s) 433, 5108, 5655
BseYI CCCAGC 3 cut(s) 1091, 5235, 5610
BsgI GTGCAG 2 cut(s) 1335, 4782
Bsh1236I CGCG 3 cut(s) 2807, 5289, 5694
BshNI GGYRCC 1 cut(s) 5105
BshVI ATCGAT 1 cut(s) 1399
BsiHKAI GWGCWC 7 cut(s) 433, 1988, 2842, 3438, 4670, 5010, 5655
BsiHKCI CYCGRG 2 cut(s) 1525, 4097
BsiSI CCGG 8 cut(s) 84, 919, 1294, 2354, 4359, 5491, 5732, 5860
BslFI GGGAC 3 cut(s) 115, 554, 5517
BslI CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 2743, 5866
BsmAI GTCTC 5 cut(s) 92, 2406, 2952, 4219, 4680
BsmBI CGTCTC 1 cut(s) 92
BsmFI GGGAC 3 cut(s) 115, 554, 5517
BsmI GAATGC 9 cut(s) 1234, 1980, 2264, 2304, 2708, 5586, 5622, 5738, 5798
BsoBI CYCGRG 2 cut(s) 1525, 4097
Bsp119I TTCGAA 2 cut(s) 2287, 4458
Bsp19I CCATGG 2 cut(s) 3001, 3165
BspCNI CTCAG 8 cut(s) 55, 2295, 2776, 3289, 3744, 4257, 4509, 5293
BspDI ATCGAT 1 cut(s) 1399
BspFNI CGCG 3 cut(s) 2807, 5289, 5694
BspHI TCATGA 2 cut(s) 535, 2097
BspLI GGNNCC 7 cut(s) 243, 570, 2184, 2563, 3810, 4836, 5107
BspMAI CTGCAG 3 cut(s) 57, 1597, 5571
BspMI ACCTGC 2 cut(s) 1307, 3961
BspOI GCTAGC 1 cut(s) 2598
BspQI GCTCTTC 2 cut(s) 2847, 5792
BspT104I TTCGAA 2 cut(s) 2287, 4458
BspT107I GGYRCC 1 cut(s) 5105
BsrDI GCAATG 3 cut(s) 2641, 3659, 4393
BsrFI RCCGGY 1 cut(s) 4358
BsrI ACTGG 7 cut(s) 1240, 1543, 2982, 3423, 4162, 4838, 5611
BssAI RCCGGY 1 cut(s) 4358
BssNI GRCGYC 2 cut(s) 5, 4627
BssSI CACGAG 4 cut(s) 2129, 3735, 4400, 5345
BssT1I CCWWGG 7 cut(s) 441, 677, 2275, 2625, 3001, 3165, 4326
Bst2BI CACGAG 4 cut(s) 2129, 3735, 4400, 5345
Bst2UI CCWGG 8 cut(s) 667, 2328, 2738, 2933, 3948, 4043, 5110, 5749
Bst6I CTCTTC 7 cut(s) 1143, 2847, 3365, 3423, 4420, 4650, 5792
BstACI GRCGYC 2 cut(s) 5, 4627
BstAPI GCANNNNNTGC 3 cut(s) 52, 958, 2453
BstBAI YACGTR 4 cut(s) 353, 2108, 3782, 4010
BstBI TTCGAA 2 cut(s) 2287, 4458
BstDSI CCRYGG 3 cut(s) 3001, 3165, 5203
BstEII GGTNACC 2 cut(s) 936, 2810
BstENI CCTNNNNNAGG 1 cut(s) 2741
BstF5I GGATG 7 cut(s) 853, 1630, 3030, 3175, 4267, 4322, 5855
BstFNI CGCG 3 cut(s) 2807, 5289, 5694
BstHHI GCGC 5 cut(s) 969, 1145, 3652, 3742, 5471
BstMAI GTCTC 5 cut(s) 92, 2406, 2952, 4219, 4680
BstNI CCWGG 8 cut(s) 667, 2328, 2738, 2933, 3948, 4043, 5110, 5749
BstNSI RCATGY 6 cut(s) 1339, 1471, 1858, 2686, 2941, 3238
BstPI GGTNACC 2 cut(s) 936, 2810
BstSFI CTRYAG 5 cut(s) 53, 1593, 4579, 4966, 5567
BstSLI GKGCMC 3 cut(s) 433, 5108, 5655
BstSNI TACGTA 1 cut(s) 2108
BstUI CGCG 3 cut(s) 2807, 5289, 5694
BstV2I GAAGAC 7 cut(s) 665, 717, 1573, 4353, 4606, 4874, 5444
BstX2I RGATCY 9 cut(s) 241, 2306, 2497, 2601, 3201, 3463, 4446, 4916, 5034
BstXI CCANNNNNNTGG 6 cut(s) 3390, 3707, 4880, 5039, 5279, 5610
BstYI RGATCY 9 cut(s) 241, 2306, 2497, 2601, 3201, 3463, 4446, 4916, 5034
Bsu15I ATCGAT 1 cut(s) 1399
BsuI GTATCC 3 cut(s) 2203, 3705, 4131
BsuTUI ATCGAT 1 cut(s) 1399
BtgI CCRYGG 3 cut(s) 3001, 3165, 5203
BtgZI GCGATG 1 cut(s) 5347
BtrI CACGTC 1 cut(s) 260
BtsCI GGATG 7 cut(s) 853, 1630, 3030, 3175, 4267, 4322, 5855
BtsI GCAGTG 2 cut(s) 50, 2579
BveI ACCTGC 2 cut(s) 1307, 3961
CaiI CAGNNNCTG 5 cut(s) 52, 1322, 3668, 3953, 4228
CciI TCATGA 2 cut(s) 535, 2097
CfoI GCGC 5 cut(s) 969, 1145, 3652, 3742, 5471
Cfr10I RCCGGY 1 cut(s) 4358
ClaI ATCGAT 1 cut(s) 1399
CseI GACGC 4 cut(s) 1340, 2813, 3054, 4635
CspCI CAANNNNNGTGG 2 cut(s) 2227, 2262
DraI TTTAAA 3 cut(s) 277, 480, 1263
DraIII CACNNNGTG 3 cut(s) 3782, 4379, 4805
DrdI GACNNNNNNGTC 1 cut(s) 2819
DriI GACNNNNNGTC 2 cut(s) 2977, 4497
DseDI GACNNNNNNGTC 1 cut(s) 2819
EaeI YGGCCR 7 cut(s) 680, 1304, 2622, 3013, 4233, 4264, 5669
Eam1104I CTCTTC 7 cut(s) 1143, 2847, 3365, 3423, 4420, 4650, 5792
Eam1105I GACNNNNNGTC 2 cut(s) 2977, 4497
EarI CTCTTC 7 cut(s) 1143, 2847, 3365, 3423, 4420, 4650, 5792
EciI GGCGGA 3 cut(s) 1666, 2372, 3214
Ecl136II GAGCTC 2 cut(s) 3436, 5008
Eco105I TACGTA 1 cut(s) 2108
Eco130I CCWWGG 7 cut(s) 441, 677, 2275, 2625, 3001, 3165, 4326
Eco24I GRGCYC 5 cut(s) 2185, 3438, 3906, 5010, 5346
Eco32I GATATC 1 cut(s) 5575
Eco47I GGWCC 6 cut(s) 568, 1434, 2081, 2521, 2971, 3950
Eco53kI GAGCTC 2 cut(s) 3436, 5008
Eco57I CTGAAG 4 cut(s) 713, 3533, 3639, 3882
Eco72I CACGTG 2 cut(s) 353, 4010
Eco88I CYCGRG 2 cut(s) 1525, 4097
Eco91I GGTNACC 2 cut(s) 936, 2810
EcoICRI GAGCTC 2 cut(s) 3436, 5008
EcoNI CCTNNNNNAGG 1 cut(s) 2741
EcoO109I RGGNCCY 1 cut(s) 3950
EcoO65I GGTNACC 2 cut(s) 936, 2810
EcoRI GAATTC 4 cut(s) 3613, 4808, 5474, 5768
EcoRII CCWGG 8 cut(s) 665, 2326, 2736, 2931, 3946, 4041, 5108, 5747
EcoRV GATATC 1 cut(s) 5575
EcoT14I CCWWGG 7 cut(s) 441, 677, 2275, 2625, 3001, 3165, 4326
EcoT22I ATGCAT 2 cut(s) 1741, 1980
EcoT38I GRGCYC 5 cut(s) 2185, 3438, 3906, 5010, 5346
ErhI CCWWGG 7 cut(s) 441, 677, 2275, 2625, 3001, 3165, 4326
Esp3I CGTCTC 1 cut(s) 92
FalI AAGNNNNNCTT 6 cut(s) 3450, 3482, 4153, 4185, 4695, 4727
FaqI GGGAC 3 cut(s) 115, 554, 5517
FauI CCCGC 3 cut(s) 2800, 4988, 5828
FauNDI CATATG 3 cut(s) 1735, 2976, 3685
FbaI TGATCA 7 cut(s) 829, 1345, 1503, 3037, 4590, 5076, 5496
FokI GGATG 7 cut(s) 860, 1637, 3037, 3182, 4254, 4309, 5842
FriOI GRGCYC 5 cut(s) 2185, 3438, 3906, 5010, 5346
FspBI CTAG 9 cut(s) 189, 252, 1389, 2595, 2847, 3192, 3560, 3813, 4175
FspI TGCGCA 1 cut(s) 3741
GlaI GCGC 5 cut(s) 968, 1144, 3651, 3741, 5470
GsaI CCCAGC 3 cut(s) 1095, 5239, 5614
GsuI CTGGAG 2 cut(s) 2963, 3225
HapII CCGG 8 cut(s) 84, 919, 1294, 2354, 4359, 5491, 5732, 5860
HgaI GACGC 4 cut(s) 1340, 2813, 3054, 4635
HhaI GCGC 5 cut(s) 969, 1145, 3652, 3742, 5471
Hin1I GRCGYC 2 cut(s) 5, 4627
Hin6I GCGC 5 cut(s) 967, 1143, 3650, 3740, 5469
HinP1I GCGC 5 cut(s) 967, 1143, 3650, 3740, 5469
HincII GTYRAC 3 cut(s) 66, 2428, 2791
HindII GTYRAC 3 cut(s) 66, 2428, 2791
HindIII AAGCTT 7 cut(s) 399, 878, 1456, 1604, 1614, 3941, 5634
HpaI GTTAAC 1 cut(s) 66
HpaII CCGG 8 cut(s) 84, 919, 1294, 2354, 4359, 5491, 5732, 5860
Hpy99I CGWCG 5 cut(s) 10, 13, 16, 2274, 5420
HpyCH4IV ACGT 9 cut(s) 259, 295, 352, 2107, 2752, 3130, 3781, 4009, 4611
HpySE526I ACGT 9 cut(s) 259, 295, 352, 2107, 2752, 3130, 3781, 4009, 4611
Hsp92I GRCGYC 2 cut(s) 5, 4627
HspAI GCGC 5 cut(s) 967, 1143, 3650, 3740, 5469
KroI GCCGGC 1 cut(s) 4358
KroNI GCCGGC 1 cut(s) 4360
Ksp22I TGATCA 7 cut(s) 829, 1345, 1503, 3037, 4590, 5076, 5496
KspAI GTTAAC 1 cut(s) 66
LguI GCTCTTC 2 cut(s) 2847, 5792
LweI GCATC 9 cut(s) 337, 787, 1299, 3018, 3488, 3721, 4331, 4809, 5275
MaeI CTAG 9 cut(s) 189, 252, 1389, 2595, 2847, 3192, 3560, 3813, 4175
MaeII ACGT 9 cut(s) 259, 295, 352, 2107, 2752, 3130, 3781, 4009, 4611
MfeI CAATTG 5 cut(s) 3888, 4029, 4566, 4823, 4829
MflI RGATCY 9 cut(s) 241, 2306, 2497, 2601, 3201, 3463, 4446, 4916, 5034
MlsI TGGCCA 6 cut(s) 682, 2624, 3015, 4235, 4266, 5671
MluNI TGGCCA 6 cut(s) 682, 2624, 3015, 4235, 4266, 5671
MlyI GAGTC 5 cut(s) 1168, 3167, 3496, 5677, 5770
MmeI TCCRAC 7 cut(s) 341, 825, 1727, 2108, 2868, 3204, 5503
Mox20I TGGCCA 6 cut(s) 682, 2624, 3015, 4235, 4266, 5671
Mph1103I ATGCAT 2 cut(s) 1741, 1980
MroNI GCCGGC 1 cut(s) 4358
MroXI GAANNNNTTC 5 cut(s) 714, 881, 3465, 4165, 4787
MscI TGGCCA 6 cut(s) 682, 2624, 3015, 4235, 4266, 5671
MslI CAYNNNNRTG 4 cut(s) 3686, 4272, 4878, 4902
Msp20I TGGCCA 6 cut(s) 682, 2624, 3015, 4235, 4266, 5671
MspA1I CMGCKG 1 cut(s) 1703
MspI CCGG 8 cut(s) 84, 919, 1294, 2354, 4359, 5491, 5732, 5860
MunI CAATTG 5 cut(s) 3888, 4029, 4566, 4823, 4829
Mva1269I GAATGC 9 cut(s) 1234, 1980, 2264, 2304, 2708, 5586, 5622, 5738, 5798
MvaI CCWGG 8 cut(s) 667, 2328, 2738, 2933, 3948, 4043, 5110, 5749
MvnI CGCG 3 cut(s) 2807, 5289, 5694
NaeI GCCGGC 1 cut(s) 4360
NciI CCSGG 3 cut(s) 85, 5732, 5860
NcoI CCATGG 2 cut(s) 3001, 3165
NdeI CATATG 3 cut(s) 1735, 2976, 3685
NgoMIV GCCGGC 1 cut(s) 4358
NheI GCTAGC 1 cut(s) 2594
NlaIV GGNNCC 7 cut(s) 243, 570, 2184, 2563, 3810, 4836, 5107
NmeAIII GCCGAG 1 cut(s) 4952
NmuCI GTSAC 8 cut(s) 1171, 1845, 1944, 2810, 2950, 3639, 4210, 5536
NsbI TGCGCA 1 cut(s) 3741
NsiI ATGCAT 2 cut(s) 1741, 1980
NspI RCATGY 6 cut(s) 1339, 1471, 1858, 2686, 2941, 3238
NspV TTCGAA 2 cut(s) 2287, 4458
OliI CACNNNNGTG 1 cut(s) 3686
PaeI GCATGC 1 cut(s) 1339
PaeR7I CTCGAG 1 cut(s) 4097
PagI TCATGA 2 cut(s) 535, 2097
PaqCI CACCTGC 1 cut(s) 1307
PciI ACATGT 1 cut(s) 2682
PciSI GCTCTTC 2 cut(s) 2847, 5792
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PctI GAATGC 9 cut(s) 1234, 1980, 2264, 2304, 2708, 5586, 5622, 5738, 5798
PdiI GCCGGC 1 cut(s) 4360
PdmI GAANNNNTTC 5 cut(s) 714, 881, 3465, 4165, 4787
PfeI GAWTC 8 cut(s) 1026, 1896, 2284, 2350, 2923, 2993, 3893, 5240
PflMI CCANNNNNTGG 1 cut(s) 1391
PfoI TCCNGGA 3 cut(s) 2931, 3946, 5858
PleI GAGTC 5 cut(s) 1168, 3166, 3496, 5677, 5770
PmaCI CACGTG 2 cut(s) 353, 4010
PmlI CACGTG 2 cut(s) 353, 4010
PpsI GAGTC 5 cut(s) 1168, 3166, 3496, 5677, 5770
Ppu21I YACGTR 4 cut(s) 353, 2108, 3782, 4010
PpuMI RGGWCCY 1 cut(s) 3950
PscI ACATGT 1 cut(s) 2682
PshBI ATTAAT 1 cut(s) 4335
PsiI TTATAA 3 cut(s) 1917, 2030, 2504
Psp124BI GAGCTC 2 cut(s) 3438, 5010
Psp5II RGGWCCY 1 cut(s) 3950
Psp6I CCWGG 8 cut(s) 665, 2326, 2736, 2931, 3946, 4041, 5108, 5747
PspCI CACGTG 2 cut(s) 353, 4010
PspEI GGTNACC 2 cut(s) 936, 2810
PspFI CCCAGC 3 cut(s) 1091, 5235, 5610
PspGI CCWGG 8 cut(s) 665, 2326, 2736, 2931, 3946, 4041, 5108, 5747
PspN4I GGNNCC 7 cut(s) 243, 570, 2184, 2563, 3810, 4836, 5107
PspPPI RGGWCCY 1 cut(s) 3950
PstI CTGCAG 3 cut(s) 57, 1597, 5571
PstNI CAGNNNCTG 5 cut(s) 52, 1322, 3668, 3953, 4228
PsuI RGATCY 9 cut(s) 241, 2306, 2497, 2601, 3201, 3463, 4446, 4916, 5034
PvuII CAGCTG 1 cut(s) 1703
RseI CAYNNNNRTG 4 cut(s) 3686, 4272, 4878, 4902
SacI GAGCTC 2 cut(s) 3438, 5010
SapI GCTCTTC 2 cut(s) 2847, 5792
SbfI CCTGCAGG 1 cut(s) 5571
ScaI AGTACT 1 cut(s) 4585
SchI GAGTC 5 cut(s) 1168, 3167, 3496, 5677, 5770
SdaI CCTGCAGG 1 cut(s) 5571
SfaNI GCATC 9 cut(s) 337, 787, 1299, 3018, 3488, 3721, 4331, 4809, 5275
SfcI CTRYAG 5 cut(s) 53, 1593, 4579, 4966, 5567
Sfr274I CTCGAG 1 cut(s) 4097
SfuI TTCGAA 2 cut(s) 2287, 4458
SinI GGWCC 6 cut(s) 568, 1434, 2081, 2521, 2971, 3950
SlaI CTCGAG 1 cut(s) 4097
SmiI ATTTAAAT 1 cut(s) 1263
SmiMI CAYNNNNRTG 4 cut(s) 3686, 4272, 4878, 4902
SmlI CTYRAG 4 cut(s) 1159, 1901, 3404, 4097
SmoI CTYRAG 4 cut(s) 1159, 1901, 3404, 4097
SnaBI TACGTA 1 cut(s) 2108
SphI GCATGC 1 cut(s) 1339
Sse8387I CCTGCAGG 1 cut(s) 5571
SspI AATATT 3 cut(s) 2158, 2244, 5276
SspMI CTAG 9 cut(s) 189, 252, 1389, 2595, 2847, 3192, 3560, 3813, 4175
SstI GAGCTC 2 cut(s) 3438, 5010
StyI CCWWGG 7 cut(s) 441, 677, 2275, 2625, 3001, 3165, 4326
SwaI ATTTAAAT 1 cut(s) 1263
TaiI ACGT 9 cut(s) 262, 298, 355, 2110, 2755, 3133, 3784, 4012, 4614
TatI WGTACW 4 cut(s) 2004, 3525, 4404, 4583
TauI GCSGC 1 cut(s) 5697
TfiI GAWTC 8 cut(s) 1026, 1896, 2284, 2350, 2923, 2993, 3893, 5240
TseFI GTSAC 8 cut(s) 1171, 1845, 1944, 2810, 2950, 3639, 4210, 5536
Tsp45I GTSAC 8 cut(s) 1171, 1845, 1944, 2810, 2950, 3639, 4210, 5536
TspGWI ACGGA 4 cut(s) 2258, 4367, 4685, 5192
Van91I CCANNNNNTGG 1 cut(s) 1391
VneI GTGCAC 2 cut(s) 429, 5651
VpaK11BI GGWCC 6 cut(s) 568, 1434, 2081, 2521, 2971, 3950
VspI ATTAAT 1 cut(s) 4335
XagI CCTNNNNNAGG 1 cut(s) 2741
XbaI TCTAGA 1 cut(s) 3559
XceI RCATGY 6 cut(s) 1339, 1471, 1858, 2686, 2941, 3238
XcmI CCANNNNNNNNNTGG 1 cut(s) 3008
XhoI CTCGAG 1 cut(s) 4097
XmnI GAANNNNTTC 5 cut(s) 714, 881, 3465, 4165, 4787
XspI CTAG 9 cut(s) 189, 252, 1389, 2595, 2847, 3192, 3560, 3813, 4175
ZrmI AGTACT 1 cut(s) 4585
Zsp2I ATGCAT 2 cut(s) 1741, 1980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.