Rroxscaffold_1G00002740

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005959
Physical Location & Seq
Reverse (-)
3261918 .. 3277287
15370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00002740.1

Sequence Viewer

Length: 5808 bp
ATGTCGTCGTCGAGAGCGGGGCCGGATCAGGGGCCGCCGCCGCAGCGGCGTATCCAGCGGACGCAGACAGCTGGAAACCTTGGGGAGGCGGCGTTCGACAGTGAGGTGGTGCCGTCATCGCTAGTCGAAATCGCTCCCATTCTTCGTGTGGCCAACGAGGTCGAATCGCACAATCCCAGAGTTGCTTATCTATGTCGATTTTATGCCTTTGAGAAAGCTCATAGGTTGGATCCCACTTCTAGTGGACGTGGTGTTCGTCAGTTCAAAACTGCTCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACTCTTATGGGAAGGGTAAAAAAAAGTGATGCACGTGAAATGCAGAGCTTCTATCAACACTATTACAAAAAATATATTCAAGCTTTGCAAAATGCGGCCGATAAGGCTGACCGCGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTATTTGAGGTTTTAAAGGCTGTTAATATGACGCAATCTATGGAAGTTGACCGTGAGATTTTGGAAGCTCATGATAAAGTTGCAGAAAAGACAGAGCTATTGGTTCCTTACAATATCCTTCCACTTGATCCTGATAGTGTAAATCAAGCAATTATGAGATACCCAGAGATTCAAGCTGCTGTTGTTGCTCTTCGTAACACCAGGGGTGATAAGGACAATGTGGCGAATCAACGGGAGCACCTGATATTGTTACTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAGCCCAAGTTGGATGACCGTGCCCTGACAGAAGTGATGAAGAAGCTTTTCAAGAATTACAAAAAATGGTGCAAGTTCTTGGGCCGGAAAAGTAGCCTTTGCTATAGCCCAGTTGGGCATGTTACCAACCATACAGCAAGAGGTGCAACAGCGCAAGCTGCTTTACATGGGTCTATATCTTTTGATATGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGTTACATTTATCATCATATGGCATTTGAATTGTATGGTATGTTAGCTGGCAATGTGAGTCCAATGACAGGAGAAAATGTGAAGCCAGCCTATGGAGGTGAAGAAGAGGCTTTCTTGAAAAAAGTTGTGACTCCTATCTATAACGTGATTGCCAGGGAAGCTGATAGGAGCAAAAGAGGGGAATCAAAGCATTCCCAGTGGAGGAACTATGATGATATAAATGAATACTTTTGGTCAGTGGATTGTTTCCGGTTGGGTTGGCCGATGCGTGCAGATGCTGATTTCTTTTGCTGCTCTAGTGAACAACGTCGTTTTGATAAAAATAGTGAGGATAACAAACCAGCTAGTGCAGATCGATGGGTGGGGAAAGTTAACTTTGTTGAGATACGGTCATTCTGGCATATCTTTAGAAGCTTTGACCGCATGTGGAGTTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAATGGCTCGGGCCAACCCACATCAATATTTTCTGCCGATGTATTCAAGAAAGCTTTGAGTGTCTTTATAACTGCTGCAATATTGAAGCTTGGACAAGCTGTTCTAGATGTAATTCTTAGCTGGAAGTCTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATATATTGCTAAGGTCATTTCAGCTGCTGCGTGGGTAATAATTCTACCGGTTACTTATGCGTATACTTGGGAAAACCCTCCTGGGTTTGCTCAGACCATTAAAGGTTGGTTTGGCAATAGTAAAAATGCGCCCTCCTTGTTCATCTTGGCTGTTGTTATCTACTTGTCACCGAATATGCTGGCTGGGGTGTTGTTTCTTTTTCCATTTATTCGCCGATTCCTTGAGAGATCAAACTATAGGATTGTGATGCTTATGATGTGGTGGTCACAGCCTCGTCTCTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCTCTTTTCAAGTACACGATGTTTTGGGTTCTCCTTGTAGTCACAAAGTTGGCATTCAGTTACTATATAGAGATAAAGCCTTTAGTGGGTCCAACAAAAGCCATCATGAATGTACGTATAACTAATTTCCAGTGGCATGAGTTCTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTGGTCTATTTTATGGATACCCAGATTTGGTATGCAATATACTCCACAATATTCGGAGGAATTTATGGTGCATTCCGTCGCCTTGGAGAGATACGGACACTAGGAATGCTAAGATCTCGCTTTGAATCATTGCCTGGTGCTTTTAACGCCCGTTTAATTCCAGCGGACAAGAGTGAGCCAAAGAAGAAAGGACTGAAGGCTACATTGTCCCGCACCTTTGGTCATGTTGAAGGTAGTAAAGACAAACAGGCTGCAAGGTTTGCGCAATTATGGAACAAAATAATAAGTAGTTTCAGAGAGGAGGATCTTATAAACAATAGGGAAATGAACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGAAATGGACCTCATACAGTGGCCTCCATTTTTACTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGCTAACAAAAAGAATCCTGGCTGATGAATACATGCATTGTGCTGTTCGTGAATGCTATGCTTCATTTAAGAACATAATTAAGTTTCTGGTTCAGGGGGACCGTGAGAAAGAGGTTATAAATTTCATATTTTCTGAGGTTGACAAGCACATAGAAGATGGTGACCTGATCCGTGAATTCAAGATGAGTGCTCTTCCTAGCCTCTATGACCACTTTGTTAGCCTTATCAACTTTTTGGGAAAGAATAGTCAGGACGACAGGGATCAAGTTGTGATTCTATTCCAGGACATGCTGGAGGTAGTGACTAGAGATATAATGATGGAGGACCATATAAACAGCTTGGTAGATTCAGTCCACGGAGGCTCAGGCCATGAGGGGATGATGCCCCTTGATCAACATCAACAGCATCAGTTGTTTGCATCTGCTGGAGCTATCAACTTTCCTCTTACACATGTAACAGAGGCTTGGAAGGAGAAGATTAACCGTCTTTTTCTATTACTTACCACAAAGGAGTCTGCCATGGACGTGCCATCCAACTTAGAAGCTAGAAGGCGAATTTCTTTCTTCTCGAATTCATTGTTTATGGATATGCCTCCAGCACCAAAAGTTCGCAATATGCTTTCTTTCTCTGTTTTAACTCCTTACTACAACGAGGAGGTTCTCTTTTCCATTGACGGTCTGGAAAAACCGAATGAAGATGGTGTTTCTATTCTCTTCTACTTGCAAAAGATTTTCCCAGATGAATGGAACAACTTTCTTGAGCGTGTGAAATGCCACAACGAAGAGGAACTAAAAGGTTCTGATGAATTGGAAGAAGATCTTCGCCTATGGGCATCATATAGAGGCCAAACTTTGACTCGTACTGTAAGAGGGATGATGTACTACCGGAAAGCTTTGGAGCTTCAGGCTTTTCTGGATATGGCCAAAGAGGAGGATTTAATGGAAGGCTATAAGGCCATAGAGTTGAATTCAGAAGATCAATCAAAGGAGGGAAGATCACTATGGACGCAATGTCAAGCAGTAGCTGATATGAAATTCACATATGTGGTTTCATGCCAAAAGTATGGTATTCAGAAGCGATCTGGTGATCTTCGTGCACAGGACATTCTGAGGCTCATGACAACATACCCATCATTACGAGTGGCATATATTGATGAGGTTGAAGAACCTAGCAAAGATAGGTCTCAGAAGATAAACCAAAAGGCTTATTATTCTACTTTGGTAAAGGCTGCTATGCCAAAGTCAATTGACTCTTCAGAGCCAGTACAAAATCTTGACCAGGTTATTTATCGCATAAAGCTTCCAGGACCTGCTATTTTGGGAGAGGGAAAGCCAGAAAATCAAAATCATGCCATTATTTTCACGCGTGGAGAAGGCTTGCAAACAATTGACATGAACCAGGATAACTACATGGAAGAAGCTTTGAAAATGAGGAATTTGCTACAAGAATTTCTTAAGCATGATGGCGTGAGGCACCCTACAATTCTTGGACTTAGGGAGCATATATTTACAGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAGAACAGTTTTGTGACTATTGGTCAAAGACTATTGGCCAACCCACTCAGGGTTCGATTTCATTATGGGCATCCCGATGTATTTGATAGGCTGTTTCACCTTAGTAGAGGGGGTGTCAGTAAGGCATCCAAGGTTATCAATTTGAGTGAAGACATTTTTGCTGGCTTTAATTCCACTCTCCGTGAAGGCAATGTAACTCATCACGAATACATACAAGTGGGGAAGGGGAGAGATGTCGGTCTTAACCAAATCTCCATGTTTGAGGCAAAAATTGCTAATGGCAACGGGGAGCAGACACTCAGTCGAGATATATACCGACTTGGACATCGTTTTGACTTTTTCCGTATGTTGTCATGTTATTTCACCACAATTGGTTTCTACTTCAGTACTCTGATCACTGTGCTTACAGTGTATGTCTTCCTTTATGGTCGCCTCTACCTGGTTCTTAGTGGACTTGAAGAAGGTTTGAACACTCAAGCAGCAATTAGAGATAATAAGCCTCTTCAAGTTGCTCTCGCTTCTCAATCATTTGTTCAAATAGGGTTTTTGATGGCTTTGCCTATGTTGATGGAAATTGGCTTGGAAAAGGGTTTCCGAACTGCATTAAGTGAATTCGTGTTGATGCAATTGCAACTGGCCCCAGTATTTTTCACATTCTCACTTGGGACAAAGACCCACTATTATGGGCGGACATTACTGCACGGTGGTGCCAAATATAGATCTACAGGTCGTGGGTTTGTGGTGTTCCATGCCAAGTTTGCCGACAACTATAGGCTCTACTCCCGCAGCCACTTTGTTAAGGGTATTGAGCTCTTGATTTTACTTGTGGTGTACCAGATCTTTGGTCATACTTATAGAAGTGCTGTTGCCTATATTTTGATCACTGTATCTATGTGGTTTATGGTGGTTACCTGGCTTTTTGCTCCCTTTCTGTTCAATCCATCTGGTTTTGAGTGGCAAAAGATTGTTGATGATTGGACTGATTGGAATAAGTGGATAAGCAACCGTGGAGGTATAGGTGTTCCACCTGAAAAAAGTTGGGAATCATGGTGGGAGGAGGAACAAGAACATCTTCGAGATTCCGGAAAACGTGGTATTGTAGTTGAGATACTGCTATCTGTACGATTCTTTATCTATCAGTATGGGCTCGTATATCACTTAAACATTGCAAAGAAAACCAAGAGTGTCCTGGTCTATGGTATCTCCTGGCTGGTGATCGTCCTTATTTTGTTTGTCATGAAGACTGTATCTGTTGGTAGGAGAAAGTTCAGTGCTGAGTACCAACTTGTATTCCGGCTGATCAAAGGATTAATATTCATTACTTTTGTGTCCATTTTGGTCACTTTGATTGTGCTGCCTCACATGACCCTGCAGGACATCATTGTTTGCATTCTTGCTTTCATGCCAACTGGTTGGGGGATGCTTATGATTGCCCAAGCTTGCAAAGGCTTGGTTCAAAAAGCTGGTTTATGGCCATCAGTTCGGACTCTTGCCCGTGGCTTTGAGATAGTTATGGGCTTGCTTCTGTTCACCCCAGTTGCATTCTTGGCCTGGTTTCCTTTTGTTTCAGAATTTCAAACTCGTATGCTCTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCTCGTATTCTTGGCGGGCAACGCAAGGATCGTTCATCCCGGAACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1935

Amino Acids

222.41

Weight (kDa)

9.12

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 44 - 171 1.9e-17 Vta1 like
FKS1_dom1 PF14288 314 - 414 3.8e-35 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 837 - 1029 4.7e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1036 - 1829 1.5e-238 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1520, 2438, 2699
Acc16I TGCGCA 1 cut(s) 2391
Acc36I ACCTGC 1 cut(s) 3898
AccB1I GGYRCC 3 cut(s) 109, 4053, 4838
AccB7I CCANNNNNTGG 1 cut(s) 1070
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 1676
AccII CGCG 2 cut(s) 420, 3946
AccIII TCCGGA 1 cut(s) 5213
AcoI YGGCCR 7 cut(s) 150, 402, 1238, 2553, 3501, 4167, 5603
AcuI CTGAAG 4 cut(s) 2342, 3467, 3819, 4498
AcvI CACGTG 1 cut(s) 341
AfaI GTAC 9 cut(s) 1940, 2040, 3442, 3461, 3846, 4519, 4964, 5253, 5411
AflII CTTAAG 1 cut(s) 4034
AflIII ACRYGT 2 cut(s) 3031, 3944
AgeI ACCGGT 1 cut(s) 1660
AhdI GACNNNNNGTC 3 cut(s) 3591, 4152, 4431
AjiI CACGTC 2 cut(s) 248, 3106
AleI CACNNNNGTG 2 cut(s) 3623, 4836
AloI GAACNNNNNNTCC 6 cut(s) 77, 109, 237, 269, 1536, 1568
Alw21I GWGCWC 6 cut(s) 696, 723, 1922, 2773, 3679, 4944
Alw26I GTCTC 2 cut(s) 1895, 3768
Alw44I GTGCAC 2 cut(s) 719, 3675
AlwNI CAGNNNCTG 4 cut(s) 1256, 1640, 3605, 3890
Ama87I CYCGRG 1 cut(s) 1459
Aor13HI TCCGGA 1 cut(s) 5213
ApaLI GTGCAC 2 cut(s) 719, 3675
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 5441
AsiGI ACCGGT 1 cut(s) 1660
Asp700I GAANNNNTTC 4 cut(s) 797, 3399, 4099, 5202
AspLEI GCGC 4 cut(s) 422, 904, 1744, 2392
AsuC2I CCSGG 1 cut(s) 5794
AsuHPI GGTGA 9 cut(s) 674, 1088, 1773, 2753, 3677, 4220, 4486, 5356, 5653
AsuNHI GCTAGC 1 cut(s) 2525
AvaI CYCGRG 1 cut(s) 1459
AvaII GGWCC 5 cut(s) 2015, 2494, 2680, 2905, 3887
BaeGI GKGCMC 3 cut(s) 723, 775, 3679
BalI TGGCCA 5 cut(s) 152, 2555, 3503, 4169, 5605
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 109, 4053, 4838
BanII GRGCYC 3 cut(s) 2119, 4944, 5280
BbrPI CACGTG 1 cut(s) 341
BbsI GAAGAC 3 cut(s) 4287, 4540, 5378
Bbv12I GWGCWC 6 cut(s) 696, 723, 1922, 2773, 3679, 4944
BceAI ACGGC 1 cut(s) 97
BciVI GTATCC 2 cut(s) 62, 2137
BclI TGATCA 5 cut(s) 745, 2971, 4524, 5010, 5430
BcnI CCSGG 1 cut(s) 5794
BcoDI GTCTC 2 cut(s) 1895, 3768
BfmI CTRYAG 5 cut(s) 853, 1849, 4854, 4900, 5501
BfrI CTTAAG 1 cut(s) 4034
BfuAI ACCTGC 1 cut(s) 3898
BfuI GTATCC 2 cut(s) 62, 2137
BglI GCCNNNNNGGC 2 cut(s) 46, 410
BglII AGATCT 4 cut(s) 2240, 3397, 4850, 4968
BmcAI AGTACT 1 cut(s) 4519
Bme18I GGWCC 5 cut(s) 2015, 2494, 2680, 2905, 3887
BmeRI GACNNNNNGTC 3 cut(s) 3591, 4152, 4431
BmeT110I CYCGRG 1 cut(s) 1459
BmgBI CACGTC 2 cut(s) 248, 3106
BmrI ACTGGG 4 cut(s) 854, 1168, 4766, 5660
BmtI GCTAGC 1 cut(s) 2529
BmuI ACTGGG 4 cut(s) 854, 1168, 4766, 5660
BpiI GAAGAC 3 cut(s) 4287, 4540, 5378
BpmI CTGGAG 3 cut(s) 2894, 3027, 3159
Bpu10I CCTNAGC 2 cut(s) 1623, 2944
BpuEI CTTGAG 3 cut(s) 1856, 3359, 4590
BpuMI CCSGG 1 cut(s) 5794
Bsa29I ATCGAT 1 cut(s) 1333
BsaAI YACGTR 2 cut(s) 341, 2042
BsaBI GATNNNNATC 1 cut(s) 2976
BsaI GGTCTC 1 cut(s) 3768
BsaWI WCCGGW 4 cut(s) 1227, 1660, 3465, 5213
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 1041, 1071, 4367, 4397
Bse118I RCCGGY 1 cut(s) 1660
Bse1I ACTGG 8 cut(s) 860, 1174, 2056, 3842, 4770, 4772, 5545, 5666
Bse3DI GCAATG 6 cut(s) 1036, 2255, 2572, 3596, 4327, 5295
Bse8I GATNNNNATC 1 cut(s) 2976
BseAI TCCGGA 1 cut(s) 5213
BseCI ATCGAT 1 cut(s) 1333
BseGI GGATG 8 cut(s) 769, 2964, 3110, 3459, 4201, 4256, 5556, 5789
BseJI GATNNNNATC 1 cut(s) 2976
BseMI GCAATG 6 cut(s) 1036, 2255, 2572, 3596, 4327, 5295
BseMII CTCAG 8 cut(s) 1718, 2706, 2958, 3680, 3779, 4192, 4444, 5397
BseNI ACTGG 8 cut(s) 860, 1174, 2056, 3842, 4770, 4772, 5545, 5666
BseRI GAGGAG 4 cut(s) 2441, 3248, 3524, 5201
BseSI GKGCMC 3 cut(s) 723, 775, 3679
BseX3I CGGCCG 1 cut(s) 402
BseYI CCCAGC 1 cut(s) 1796
BsgI GTGCAG 3 cut(s) 1269, 1347, 4814
Bsh1236I CGCG 2 cut(s) 420, 3946
Bsh1285I CGRYCG 1 cut(s) 405
BshNI GGYRCC 3 cut(s) 109, 4053, 4838
BshTI ACCGGT 1 cut(s) 1660
BshVI ATCGAT 1 cut(s) 1333
BsiEI CGRYCG 1 cut(s) 405
BsiHKAI GWGCWC 6 cut(s) 696, 723, 1922, 2773, 3679, 4944
BsiHKCI CYCGRG 1 cut(s) 1459
BsiSI CCGG 8 cut(s) 23, 835, 1228, 1661, 3466, 5214, 5425, 5794
BslFI GGGAC 3 cut(s) 2320, 2693, 4810
BsmAI GTCTC 2 cut(s) 1895, 3768
BsmBI CGTCTC 1 cut(s) 1895
BsmFI GGGAC 3 cut(s) 2320, 2693, 4810
Bso31I GGTCTC 1 cut(s) 3768
BsoBI CYCGRG 1 cut(s) 1459
Bsp1286I GDGCHC 9 cut(s) 696, 723, 775, 1922, 2119, 2773, 3679, 4944, 5280
Bsp13I TCCGGA 1 cut(s) 5213
Bsp19I CCATGG 1 cut(s) 3099
BspCNI CTCAG 8 cut(s) 1717, 2707, 2957, 3681, 3778, 4191, 4443, 5398
BspDI ATCGAT 1 cut(s) 1333
BspEI TCCGGA 1 cut(s) 5213
BspFNI CGCG 2 cut(s) 420, 3946
BspHI TCATGA 4 cut(s) 526, 2031, 3696, 5367
BspMAI CTGCAG 1 cut(s) 5505
BspMI ACCTGC 1 cut(s) 3898
BspOI GCTAGC 1 cut(s) 2529
BspQI GCTCTTC 4 cut(s) 279, 651, 2778, 5726
BspT107I GGYRCC 3 cut(s) 109, 4053, 4838
BspTI CTTAAG 1 cut(s) 4034
BspTNI GGTCTC 1 cut(s) 3768
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 6 cut(s) 1036, 2255, 2572, 3596, 4327, 5295
BsrFI RCCGGY 1 cut(s) 1660
BsrI ACTGG 8 cut(s) 860, 1174, 2056, 3842, 4770, 4772, 5545, 5666
BssAI RCCGGY 1 cut(s) 1660
BssNAI GTATAC 1 cut(s) 1677
BssT1I CCWWGG 6 cut(s) 79, 432, 2209, 2556, 3099, 4260
Bst1107I GTATAC 1 cut(s) 1677
Bst6I CTCTTC 9 cut(s) 279, 651, 1077, 2778, 3299, 3357, 3838, 4638, 5726
BstAFI CTTAAG 1 cut(s) 4034
BstAPI GCANNNNNTGC 3 cut(s) 893, 2387, 4403
BstBAI YACGTR 2 cut(s) 341, 2042
BstDSI CCRYGG 4 cut(s) 2935, 3099, 5137, 5626
BstEII GGTNACC 2 cut(s) 2741, 5038
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 8 cut(s) 769, 2964, 3110, 3459, 4201, 4256, 5556, 5789
BstFNI CGCG 2 cut(s) 420, 3946
BstHHI GCGC 4 cut(s) 422, 904, 1744, 2392
BstMAI GTCTC 2 cut(s) 1895, 3768
BstMCI CGRYCG 1 cut(s) 405
BstNSI RCATGY 5 cut(s) 872, 1405, 2617, 2872, 3035
BstPI GGTNACC 2 cut(s) 2741, 5038
BstSFI CTRYAG 5 cut(s) 853, 1849, 4854, 4900, 5501
BstSLI GKGCMC 3 cut(s) 723, 775, 3679
BstSNI TACGTA 1 cut(s) 2042
BstUI CGCG 2 cut(s) 420, 3946
BstV2I GAAGAC 3 cut(s) 4287, 4540, 5378
BstX2I RGATCY 7 cut(s) 229, 2240, 2431, 2532, 3397, 4850, 4968
BstXI CCANNNNNNTGG 5 cut(s) 3324, 3644, 4814, 4973, 5544
BstYI RGATCY 7 cut(s) 229, 2240, 2431, 2532, 3397, 4850, 4968
BstZ17I GTATAC 1 cut(s) 1677
BstZI CGGCCG 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 1333
BsuI GTATCC 2 cut(s) 62, 2137
BsuTUI ATCGAT 1 cut(s) 1333
BtgI CCRYGG 4 cut(s) 2935, 3099, 5137, 5626
BtgZI GCGATG 1 cut(s) 102
BtrI CACGTC 2 cut(s) 248, 3106
BtsCI GGATG 8 cut(s) 769, 2964, 3110, 3459, 4201, 4256, 5556, 5789
BtsIMutI CAGTG 9 cut(s) 106, 1181, 1221, 2063, 2510, 4527, 4545, 5013, 5407
BveI ACCTGC 1 cut(s) 3898
CaiI CAGNNNCTG 4 cut(s) 1256, 1640, 3605, 3890
CciI TCATGA 4 cut(s) 526, 2031, 3696, 5367
CfoI GCGC 4 cut(s) 422, 904, 1744, 2392
Cfr10I RCCGGY 1 cut(s) 1660
ClaI ATCGAT 1 cut(s) 1333
CseI GACGC 3 cut(s) 70, 496, 3595
CsiI ACCWGGT 2 cut(s) 3858, 4569
Csp6I GTAC 9 cut(s) 1939, 2039, 3441, 3460, 3845, 4518, 4963, 5252, 5410
CspAI ACCGGT 1 cut(s) 1660
CviQI GTAC 9 cut(s) 1939, 2039, 3441, 3460, 3845, 4518, 4963, 5252, 5410
DraI TTTAAA 1 cut(s) 471
DriI GACNNNNNGTC 3 cut(s) 3591, 4152, 4431
EaeI YGGCCR 7 cut(s) 150, 402, 1238, 2553, 3501, 4167, 5603
EagI CGGCCG 1 cut(s) 402
Eam1104I CTCTTC 9 cut(s) 279, 651, 1077, 2778, 3299, 3357, 3838, 4638, 5726
Eam1105I GACNNNNNGTC 3 cut(s) 3591, 4152, 4431
EarI CTCTTC 9 cut(s) 279, 651, 1077, 2778, 3299, 3357, 3838, 4638, 5726
EciI GGCGGA 2 cut(s) 1600, 4834
Ecl136II GAGCTC 1 cut(s) 4942
EclXI CGGCCG 1 cut(s) 402
Eco105I TACGTA 1 cut(s) 2042
Eco130I CCWWGG 6 cut(s) 79, 432, 2209, 2556, 3099, 4260
Eco24I GRGCYC 3 cut(s) 2119, 4944, 5280
Eco31I GGTCTC 1 cut(s) 3768
Eco47I GGWCC 5 cut(s) 2015, 2494, 2680, 2905, 3887
Eco52I CGGCCG 1 cut(s) 402
Eco53kI GAGCTC 1 cut(s) 4942
Eco57I CTGAAG 4 cut(s) 2342, 3467, 3819, 4498
Eco72I CACGTG 1 cut(s) 341
Eco88I CYCGRG 1 cut(s) 1459
Eco91I GGTNACC 2 cut(s) 2741, 5038
EcoICRI GAGCTC 1 cut(s) 4942
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 3887
EcoO65I GGTNACC 2 cut(s) 2741, 5038
EcoRI GAATTC 4 cut(s) 2756, 3151, 3547, 4742
EcoT14I CCWWGG 6 cut(s) 79, 432, 2209, 2556, 3099, 4260
EcoT22I ATGCAT 2 cut(s) 1914, 2619
EcoT38I GRGCYC 3 cut(s) 2119, 4944, 5280
ErhI CCWWGG 6 cut(s) 79, 432, 2209, 2556, 3099, 4260
Esp3I CGTCTC 1 cut(s) 1895
FalI AAGNNNNNCTT 8 cut(s) 3384, 3416, 4017, 4049, 4087, 4119, 5187, 5219
FaqI GGGAC 3 cut(s) 2320, 2693, 4810
FauI CCCGC 4 cut(s) 10, 2345, 4922, 5762
FauNDI CATATG 2 cut(s) 996, 3622
FbaI TGATCA 5 cut(s) 745, 2971, 4524, 5010, 5430
FblI GTMKAC 1 cut(s) 1676
FokI GGATG 8 cut(s) 776, 2971, 3097, 3466, 4188, 4243, 5563, 5776
FriOI GRGCYC 3 cut(s) 2119, 4944, 5280
FspI TGCGCA 1 cut(s) 2391
GlaI GCGC 4 cut(s) 421, 903, 1743, 2391
GsaI CCCAGC 1 cut(s) 1800
GsuI CTGGAG 3 cut(s) 2894, 3027, 3159
HapII CCGG 8 cut(s) 23, 835, 1228, 1661, 3466, 5214, 5425, 5794
HgaI GACGC 3 cut(s) 70, 496, 3595
HhaI GCGC 4 cut(s) 422, 904, 1744, 2392
Hin6I GCGC 4 cut(s) 420, 902, 1742, 2390
HinP1I GCGC 4 cut(s) 420, 902, 1742, 2390
HincII GTYRAC 3 cut(s) 505, 1351, 2722
HindII GTYRAC 3 cut(s) 505, 1351, 2722
HindIII AAGCTT 9 cut(s) 387, 794, 1390, 1503, 1538, 3471, 3878, 3999, 5568
HpaI GTTAAC 1 cut(s) 1351
HpaII CCGG 8 cut(s) 23, 835, 1228, 1661, 3466, 5214, 5425, 5794
HphI GGTGA 9 cut(s) 674, 1088, 1773, 2753, 3677, 4220, 4486, 5356, 5653
Hpy99I CGWCG 4 cut(s) 10, 13, 1290, 2208
HpyCH4IV ACGT 8 cut(s) 247, 283, 340, 1122, 1285, 2041, 3105, 5221
HpySE526I ACGT 8 cut(s) 247, 283, 340, 1122, 1285, 2041, 3105, 5221
HspAI GCGC 4 cut(s) 420, 902, 1742, 2390
Kpn2I TCCGGA 1 cut(s) 5213
Ksp22I TGATCA 5 cut(s) 745, 2971, 4524, 5010, 5430
KspAI GTTAAC 1 cut(s) 1351
LguI GCTCTTC 4 cut(s) 279, 651, 2778, 5726
LmnI GCTCC 9 cut(s) 139, 691, 1146, 2122, 3008, 3478, 4078, 4420, 5059
MabI ACCWGGT 2 cut(s) 3858, 4569
MaeII ACGT 8 cut(s) 247, 283, 340, 1122, 1285, 2041, 3105, 5221
MbiI CCGCTC 1 cut(s) 17
MfeI CAATTG 4 cut(s) 3825, 3966, 4500, 4757
MflI RGATCY 7 cut(s) 229, 2240, 2431, 2532, 3397, 4850, 4968
MhlI GDGCHC 9 cut(s) 696, 723, 775, 1922, 2119, 2773, 3679, 4944, 5280
MlsI TGGCCA 5 cut(s) 152, 2555, 3503, 4169, 5605
MluI ACGCGT 1 cut(s) 3944
MluNI TGGCCA 5 cut(s) 152, 2555, 3503, 4169, 5605
MlyI GAGTC 6 cut(s) 1045, 1102, 3101, 3430, 3824, 5611
MmeI TCCRAC 5 cut(s) 207, 329, 741, 2042, 3138
Mox20I TGGCCA 5 cut(s) 152, 2555, 3503, 4169, 5605
Mph1103I ATGCAT 2 cut(s) 1914, 2619
MroI TCCGGA 1 cut(s) 5213
MroXI GAANNNNTTC 4 cut(s) 797, 3399, 4099, 5202
MscI TGGCCA 5 cut(s) 152, 2555, 3503, 4169, 5605
MslI CAYNNNNRTG 6 cut(s) 3104, 3623, 4206, 4346, 4812, 4836
Msp20I TGGCCA 5 cut(s) 152, 2555, 3503, 4169, 5605
MspA1I CMGCKG 5 cut(s) 46, 58, 71, 1637, 2291
MspCI CTTAAG 1 cut(s) 4034
MspI CCGG 8 cut(s) 23, 835, 1228, 1661, 3466, 5214, 5425, 5794
MunI CAATTG 4 cut(s) 3825, 3966, 4500, 4757
MvnI CGCG 2 cut(s) 420, 3946
NciI CCSGG 1 cut(s) 5794
NcoI CCATGG 1 cut(s) 3099
NdeI CATATG 2 cut(s) 996, 3622
NheI GCTAGC 1 cut(s) 2525
NmuCI GTSAC 8 cut(s) 1105, 1779, 1878, 1966, 2741, 2881, 4144, 5470
NsbI TGCGCA 1 cut(s) 2391
NsiI ATGCAT 2 cut(s) 1914, 2619
NspI RCATGY 5 cut(s) 872, 1405, 2617, 2872, 3035
OliI CACNNNNGTG 2 cut(s) 3623, 4836
PagI TCATGA 4 cut(s) 526, 2031, 3696, 5367
PciI ACATGT 1 cut(s) 3031
PciSI GCTCTTC 4 cut(s) 279, 651, 2778, 5726
PcsI WCGNNNNNNNCGW 2 cut(s) 253, 5782
PdmI GAANNNNTTC 4 cut(s) 797, 3399, 4099, 5202
PflMI CCANNNNNTGG 1 cut(s) 1070
PfoI TCCNGGA 3 cut(s) 2862, 3883, 5792
PinAI ACCGGT 1 cut(s) 1660
PleI GAGTC 6 cut(s) 1044, 1102, 3100, 3430, 3824, 5611
PmaCI CACGTG 1 cut(s) 341
PmlI CACGTG 1 cut(s) 341
PpsI GAGTC 6 cut(s) 1044, 1102, 3100, 3430, 3824, 5611
Ppu21I YACGTR 2 cut(s) 341, 2042
PpuMI RGGWCCY 1 cut(s) 3887
PscI ACATGT 1 cut(s) 3031
PshBI ATTAAT 1 cut(s) 5441
PsiI TTATAA 3 cut(s) 1520, 2438, 2699
Psp124BI GAGCTC 1 cut(s) 4944
Psp5II RGGWCCY 1 cut(s) 3887
PspCI CACGTG 1 cut(s) 341
PspEI GGTNACC 2 cut(s) 2741, 5038
PspFI CCCAGC 1 cut(s) 1796
PspPPI RGGWCCY 1 cut(s) 3887
PstI CTGCAG 1 cut(s) 5505
PstNI CAGNNNCTG 4 cut(s) 1256, 1640, 3605, 3890
PsuI RGATCY 7 cut(s) 229, 2240, 2431, 2532, 3397, 4850, 4968
PvuII CAGCTG 2 cut(s) 71, 1637
RsaI GTAC 9 cut(s) 1940, 2040, 3442, 3461, 3846, 4519, 4964, 5253, 5411
RsaNI GTAC 9 cut(s) 1939, 2039, 3441, 3460, 3845, 4518, 4963, 5252, 5410
RseI CAYNNNNRTG 6 cut(s) 3104, 3623, 4206, 4346, 4812, 4836
SacI GAGCTC 1 cut(s) 4944
SapI GCTCTTC 4 cut(s) 279, 651, 2778, 5726
SbfI CCTGCAGG 1 cut(s) 5505
ScaI AGTACT 1 cut(s) 4519
SchI GAGTC 6 cut(s) 1045, 1102, 3101, 3430, 3824, 5611
SdaI CCTGCAGG 1 cut(s) 5505
SduI GDGCHC 9 cut(s) 696, 723, 775, 1922, 2119, 2773, 3679, 4944, 5280
SexAI ACCWGGT 2 cut(s) 3858, 4569
SfcI CTRYAG 5 cut(s) 853, 1849, 4854, 4900, 5501
SinI GGWCC 5 cut(s) 2015, 2494, 2680, 2905, 3887
SmiMI CAYNNNNRTG 6 cut(s) 3104, 3623, 4206, 4346, 4812, 4836
SmlI CTYRAG 4 cut(s) 1835, 3338, 4034, 4605
SmoI CTYRAG 4 cut(s) 1835, 3338, 4034, 4605
SnaBI TACGTA 1 cut(s) 2042
Sse8387I CCTGCAGG 1 cut(s) 5505
SspI AATATT 4 cut(s) 1479, 1533, 2178, 5445
SstI GAGCTC 1 cut(s) 4944
StyI CCWWGG 6 cut(s) 79, 432, 2209, 2556, 3099, 4260
TaiI ACGT 8 cut(s) 250, 286, 343, 1125, 1288, 2044, 3108, 5224
TaqII GACCGA 1 cut(s) 4358
TatI WGTACW 4 cut(s) 1938, 3459, 3844, 4517
TauI GCSGC 6 cut(s) 37, 40, 43, 49, 92, 404
TscAI CASTG 9 cut(s) 106, 1181, 1221, 2063, 2510, 4534, 4545, 5020, 5407
TseFI GTSAC 8 cut(s) 1105, 1779, 1878, 1966, 2741, 2881, 4144, 5470
Tsp45I GTSAC 8 cut(s) 1105, 1779, 1878, 1966, 2741, 2881, 4144, 5470
TspGWI ACGGA 6 cut(s) 2192, 2236, 2741, 2952, 4301, 4463
TspRI CASTG 9 cut(s) 106, 1181, 1221, 2063, 2510, 4534, 4545, 5020, 5407
Van91I CCANNNNNTGG 1 cut(s) 1070
Vha464I CTTAAG 1 cut(s) 4034
VneI GTGCAC 2 cut(s) 719, 3675
VpaK11BI GGWCC 5 cut(s) 2015, 2494, 2680, 2905, 3887
VspI ATTAAT 1 cut(s) 5441
XagI CCTNNNNNAGG 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 1555
XceI RCATGY 5 cut(s) 872, 1405, 2617, 2872, 3035
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 3256, 5317
XmiI GTMKAC 1 cut(s) 1676
XmnI GAANNNNTTC 4 cut(s) 797, 3399, 4099, 5202
ZrmI AGTACT 1 cut(s) 4519
Zsp2I ATGCAT 2 cut(s) 1914, 2619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.