Rw5G048640

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005959
Physical Location & Seq
Forward (+)
84523553 .. 84537113
13561 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048640.1

Sequence Viewer

Length: 5802 bp
ATGTCGTCGTCGAGAGCGGGGCCGGATCAGGGGCCGCCGCCGCAGCGGCGTATCCAGCGGACGCAGACAGCTGGAAACCTCGGGGAGGCGGCTTTCGACAGTGAGGTGGTGCCGTCATCCCTCGTCGAAATCGCTCCCATTCTTCGTGTGGCCAACGAGGTCGAATCGCACAATCCCAGAGTTGCTTATCTATGTCGATTTTATGCCTTTGAGAAAGCTCATAGGTTGGATCCCACTTCTAGTGGACGTGGTGTTCGTCAGTTCAAAACTGCTCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACTCTTATGGGAAGGGTAAAAAAAAGTGATGCACGTGAAATGCAGAGCTTCTATCAACACTATTACAAAAAATATATTCAAGCTTTGCAAAATGCAGCCGATAAAGCTGACCGTGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTATTTGAGGTTTTAAAGGCTGTTAATATGACACAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGATAAAGTTGCAGAAAAGACAGAGCTATTGGTTCCTTACAATATCCTTCCACTTGATCCTGATAGTGTAAATCAAGCAATTATGAGATACCCAGAGATTCAAGCTGCTGTTGTTGCTCTTCGTAACACCAGGGGTCTTCCTTGGCCTAAGGAATACAAAAAGAAAAAAGACGAGGATGTTCTTGATTGGCTGCAGTCAATGTTCGGGTTTCAGAAGGACAATGTGGCGAATCAACGGGAGCACCTGATATTGTTACTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAGCCCAAGTTGGATGACCGTGCCCTGACAGAAGTGATGAAGAAGCTTTTCAAGAATTACAAAAAATGGTGCAAGTTCTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACCATACAGCAAGAGGTGCAACAGCGCAAGCTGCTTTACATGGGTCTATATCTTTTGATATGGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGTTACATTTATCATCATAATAGTATTTGGTTAGGTGAAGAAGAGGCTTTCTTGAAAAAAGTTGTGACTCCTATCTATAACGTGATTGCCAGGGAAGCTGATAGGAGCAAAAGAGGGGAATCAAAGCATTCCCAGTGGAGGAACTATGATGATATAAATGAATACTTTTGGTCAGTGGATTGTTTCCGGTTGGGTTGGCCGATGCGTGCAGATGCTGATTTCTTTTGCTGCTCTAGTGAACAACGTCATTTTGATAAAAATAGTGAGGATAACAAACCAGCTAGTGGAGATCGATGGGTGGGGAAAGTTAACTTTGTTGAGATACGGTCATTCTGGCATATCTTTAGAAGCTTTGACCGCATGTGGAGTTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAATGGCTCGGGCCAACCCACATCAATATTTTCTGCCGATGTATTCAAGAAAGCTTTGAGTGTCTTTATAACTGCTGCAATATTGAAGCTTGGACAAGCTGTTCTAGATGTAATTCTTAGCTGGAAGTCTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATATATTGCTAAGGTCATTTCAGCTGCTGCGTGGGTAATAATTCTACCGGTTACTTATGCGTATACTTGGGAAAACCCTCCTGGGTTTGCTCAGACCATTAAAGGTTGGTTTGGCAATAGTAAAAACGCGCCCTCCTTGTTCATCTTGGCTGTTGTTATCTACTTGTCACCGAATATGCTGGCTGGGGTGTTGTTTCTTTTCCCATTTATTCGCCGATTCCTTGAGAGATCAAACTATAGGATTGTGATGCTTATGATGTGGTGGTCACAGCCTCGTCTCTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCTCTTTTCAAGTACACGATGTTTTGGGTTCTCCTTGTAGTCACAAAGTTGGCATTCAGTTACTATATAGAGATAAAGCCTTTAGTGGGTCCAACAAAAGCCATCATGAATGTACGTATAACTAATTTCCAGTGGCATGAGTTCTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTGGTCTATTTTATGGATACCCAGATTTGGTATGCAATATACTCCACAATATTCGGAGGAATTTATGGTGCATTCCGTCGCCTTGGAGAGATACGGACACTAGGAATGCTAAGATCTCGCTTTGAATCATTGCCTGGTGCTTTTAACGCCCGTTTAATTCCAGCGGACAAGAGTGAGCCAAAGAAGAAAGGACTGAAGGCTACATTGTCCCGCACCTTTGGTCATGTTGAAGGTAGTAAAGACAAACAGGCTGCAAGGTTTGCGCAATTATGGAACAAAATAATAAGTAGTTTCAGAGAGGAGGATCTTATAAACCAAAGGGAAATGAACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGAAATGGACCTCATACGGTGGCCTCCATTTTTACTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGCTAACAAAAAGAATCCTGGCTGATGAATACATGCATTGTGCTGTTCGCGAATGCTATGCTTCATTTAAGAACATAATTAAGTTTCTGGTTCAGGGGGACCGTGAGAAAGAGGTTATAAATTCCATATTTTCTGAGGTTGACAAGCACATAGAAGATGGTGACCTGATCCGTGAATTGAAGATGAGTGCTCTTCCTAGCCTCTATGACCACTTTGTTCGCCTTATCAACTTTTTGAGCAAGAATAGTCAGGACGACAGGGATCAAGTTGTGATTCTATTCCAGGACATGCTGGAGGTAGTGACTAGAGATATAATGATGGAGGAGCAGATAAACTGCTTGGTAGATTCAGTCCACGGAGGCTCGGGCCATGAGGGGATGATGCCCCTTGATCAACATCAACAGCATCAGTTGTTTGCATCTGCTGGAGCTATCAACTTTCCTCTTACACATGTAACAGAGGCTTGGAAGGAGAAGATTAACCGTCTTTTTCTATTACTTACCACAAAGGAGTCTGCCATGGACGTGCCATCCAACTTAGAAGCTAGAAGGCGAATTTCTTTCTTCTCGAATTCATTGTTTATGGATATGCCTCCAGCACCAAAAGTTCGCAATATGCTTTCTTTCTCTGTTTTAACTCCTTACTACAACGAGGAGGTTCTCTTTTCCATTGACGGTCTGGAAAAACCGAATGAAGATGGTGTTTCTATTCTCTTCTACTTGCAAAAGATTTTCCCAGATGAATGGAACAACTTTCTTGAGCGTGTGAATTGCAACAACGAAGAGGAACTAAAAGGTTCTGATGAATTGGAAGAAGAACTTCGCCTATGGGCATCATATAGAGGCCAAACTTTGACTCGTACTGTAAGAGGGATGATGTACTACCGGAAAGCTTTGGAGCTTCAGGCTTTTCTGGATATGGCCAAAGAGGAGGATTTAATGGAAGGCTATAAGGCCATAGAGTTGAATTCAGAAGATCAATCAAAGGAGGGAAGATCACTATGGGCGCAATGTCAAGCAGTAGCTGATATGAAATTCACATATGTGGTTTCATGCCAAAAGTATGGTATTCAGAAGCGATCTGGTGATCTTCGTGCACAGGACATTCTGAGGCTCATGACAACATACCCATCATTACGAGTGGCATATATTGATGAGGTTGAAGAACCTAGCAAAGATAGGTCTCAGAAGATAAACCAAAAGGCTTATTATTCTACTTTGGTAAAGGCTGCTATGCCAAAGTCAATTGACCCTTCAGAGCCAGTACAAAATCTTGACCAGGTTATTTATCGCATAAAGCTTCCAGGACCTGCTATTTTGGGAGAGGGAAAGCCAGAAAATCAAAATCATGCCATTATTTTCACGCGTGGTGAAGGCTTGCAAACAATTGACATGAACCAGGATAACTACATGGAAGAAGCTTTGAAAATGAGGAATTTGCTGCAAGAATTTCTTAAGCATGATGGCGTGAGGCACCCTACAATTCTTGGACTTAGGGAGCATATATTTACAGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAGAACAGTTTTGTGACTATTGGTCAAAGACTATTGGCCAACCCACTCAGGGTTCGATTTCATTATGGGCATCCCGATGTATTTGATAGGCTCTTTCACCTTAGTAGAGGGGGTGTTAGTAAGGCATCCAAGGTTATCAATTTGAGTGAAGACATTTTTGCTGGCTTTAATTCCACTCTCCGTGAAGGCAATGTAACTCATCACGAATACATACAAGTGGGGAAGGGGAGAGATGTCGGTCTTAACCAAATCTCCATGTTTGAGGCAAAAATTGCTAATGGCAACGGGGAGCAGACACTCAGTCGAGATATATACCGACTTGGACATCGTTTTGACTTTTTCCGTATGTTGTCATGTTATTTCACCACAATTGGTTTCTACTTCAGTACTCTGATCACTGTGCTTACAGTGTATGTCTTCCTTTATGGTCGCCTCTACCTGGTTCTTAGTGGACTTGAAGAAGGTTTGAACACTCAAGCAGCAATTCGAGATAATAAGCCTCTTCAAGTTGCTCTCGCTTCTCAATCATTTGTTCAAATAGGGTTTTTGATGGCTTTGCCTATGTTGATGGAAATTGGCTTGGAAAAGGGTTTCCGAACTGCATTAAGTGAATTCGTGTTGATGCAATTGCAACTGGCCCCAGTATTTTTCACATTCTCACTTGGGACAAAGACCCACTATTATGGACGGACATTACTGCATGGTGGTGCCAAATATAGATCTACAGGTCGTGGGTTTGTGGTGTTCCATGCCAAGTTTGCCGACAACTATAGGCTCTACTCCCGCAGCCACTTTGTTAAGGGTATTGAGCTCTTGATTTTACTTGTGGTGTACCAGATCTTTGGTCATACTTACAGAAGTGCTGTTGCCTACATTTTGATCACTGTATCTATGTGGTTTATGGTGGTTACCTGGCTTTTTGCTCCCTTTCTGTTCAATCCATCTGGTTTTGAGTGGCAAAAGATTGTTGATGATTGGACTGATTGGAATAAGTGGATAAGCAACCGTGGAGGTATAGGTGTTCCGCCTGAAAAAAGTTGGGAATCATGGTGGGAGGAGGAACAAGAACATCTTCGATATTCCGGAAAACGTGGCATTGTAGTTGAGATACTGCTATCTGTACGATTCTTTATCTATCAGTATGGGCTTGTATATCACTTAAACATTGCAAAGAAAACCAAGAGTGTCCTGGTCTATGGTATCTCATGGCTGGTGATCGTCCTTATTTTGTTTGTCATGAAGACTGTATCTGTTGGTAGGAGAAAGTTCAGTGCTGAGTACCAACTTGTATTCCGGCTGATCAAGGGATTAATATTCATTACTTTTGTATCCATTTTGGTCACTTTGATTGTGCTGCCTCACATGACCCTGCAGGACATCATTGTTTGCATTCTTGCTTTCATGCCAACTGGTTGGGGCATGCTTATGATTGCCCAAGCTTGCAAAGGCTTGGTTCACAAAGCTGGTTTATGGCCATCAGTTCGGACTCTTGCCCGTGGCTTTGAGATAGTTATGGGCTTGCTTCTGTTCACCCCAGTTGCATTCTTGGCCTGGTTTCCTTTTGTTTCGGAATTTCAAACTCGTATGCTCTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCTCGTATTCTTGGCGGACAACGCAAGGATCGTTCATCCCGGAACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1933

Amino Acids

223.4

Weight (kDa)

9.19

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 44 - 171 1.9e-17 Vta1 like
FKS1_dom1 PF14288 321 - 412 4.6e-34 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 835 - 1027 2.2e-65 Callose synthase 1 domain
Glucan_synthase PF02364 1034 - 1827 1.4e-238 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1514, 2432, 2693
Acc16I TGCGCA 1 cut(s) 2385
Acc36I ACCTGC 1 cut(s) 3892
AccB1I GGYRCC 3 cut(s) 109, 4047, 4832
AccB7I CCANNNNNTGG 1 cut(s) 1319
AccBSI CCGCTC 1 cut(s) 17
AccI GTMKAC 1 cut(s) 1670
AccII CGCG 3 cut(s) 1736, 2625, 3940
AccIII TCCGGA 1 cut(s) 5207
AcoI YGGCCR 6 cut(s) 150, 1232, 2547, 3495, 4161, 5597
AcuI CTGAAG 4 cut(s) 2336, 3461, 3813, 4492
AcvI CACGTG 1 cut(s) 341
AfaI GTAC 9 cut(s) 1934, 2034, 3436, 3455, 3840, 4513, 4958, 5247, 5405
AfiI CCNNNNNNNGG 9 cut(s) 29, 85, 1173, 1319, 2006, 2148, 4174, 4564, 5794
AflII CTTAAG 1 cut(s) 4028
AflIII ACRYGT 2 cut(s) 3025, 3938
AgeI ACCGGT 1 cut(s) 1654
AhdI GACNNNNNGTC 2 cut(s) 4146, 4425
AjiI CACGTC 2 cut(s) 248, 3100
AleI CACNNNNGTG 1 cut(s) 3617
AloI GAACNNNNNNTCC 4 cut(s) 237, 269, 1530, 1562
Alw21I GWGCWC 7 cut(s) 424, 771, 798, 1916, 2767, 3673, 4938
Alw26I GTCTC 2 cut(s) 1889, 3762
Alw44I GTGCAC 3 cut(s) 420, 794, 3669
AlwNI CAGNNNCTG 4 cut(s) 1250, 1634, 3599, 3884
Ama87I CYCGRG 3 cut(s) 80, 1453, 2938
Aor13HI TCCGGA 1 cut(s) 5207
ApaLI GTGCAC 3 cut(s) 420, 794, 3669
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
AseI ATTAAT 1 cut(s) 5435
AsiGI ACCGGT 1 cut(s) 1654
Asp700I GAANNNNTTC 4 cut(s) 872, 3393, 4093, 5196
AspLEI GCGC 4 cut(s) 960, 1738, 2386, 3583
AsuC2I CCSGG 1 cut(s) 5788
AsuHPI GGTGA 9 cut(s) 1082, 1767, 2747, 3671, 3956, 4214, 4480, 5350, 5647
AsuNHI GCTAGC 1 cut(s) 2519
AvaI CYCGRG 3 cut(s) 80, 1453, 2938
AvaII GGWCC 4 cut(s) 2009, 2488, 2674, 3881
AxyI CCTNAGG 1 cut(s) 675
BaeGI GKGCMC 4 cut(s) 424, 798, 850, 3673
BaeI ACNNNNGTAYC 2 cut(s) 5436, 5469
BalI TGGCCA 5 cut(s) 152, 2549, 3497, 4163, 5599
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 3 cut(s) 109, 4047, 4832
BanII GRGCYC 2 cut(s) 2113, 4938
BbrPI CACGTG 1 cut(s) 341
BbsI GAAGAC 4 cut(s) 656, 4281, 4534, 5372
Bbv12I GWGCWC 7 cut(s) 424, 771, 798, 1916, 2767, 3673, 4938
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 2615, 2649
BciVI GTATCC 3 cut(s) 62, 2131, 5464
BclI TGATCA 5 cut(s) 820, 2965, 4518, 5004, 5424
BcnI CCSGG 1 cut(s) 5788
BcoDI GTCTC 2 cut(s) 1889, 3762
BfmI CTRYAG 5 cut(s) 719, 1843, 4848, 4894, 5495
BfrI CTTAAG 1 cut(s) 4028
BfuAI ACCTGC 1 cut(s) 3892
BfuI GTATCC 3 cut(s) 62, 2131, 5464
BglI GCCNNNNNGGC 1 cut(s) 46
BglII AGATCT 3 cut(s) 2234, 4844, 4962
BmcAI AGTACT 1 cut(s) 4513
Bme18I GGWCC 4 cut(s) 2009, 2488, 2674, 3881
BmeRI GACNNNNNGTC 2 cut(s) 4146, 4425
BmeT110I CYCGRG 3 cut(s) 80, 1453, 2938
BmgBI CACGTC 2 cut(s) 248, 3100
BmrI ACTGGG 3 cut(s) 1162, 4760, 5654
BmtI GCTAGC 1 cut(s) 2523
BmuI ACTGGG 3 cut(s) 1162, 4760, 5654
BpiI GAAGAC 4 cut(s) 656, 4281, 4534, 5372
BpmI CTGGAG 3 cut(s) 2888, 3021, 3153
Bpu10I CCTNAGC 1 cut(s) 1617
BpuEI CTTGAG 3 cut(s) 1850, 3353, 4584
BpuMI CCSGG 1 cut(s) 5788
Bsa29I ATCGAT 1 cut(s) 1327
BsaAI YACGTR 2 cut(s) 341, 2036
BsaBI GATNNNNATC 1 cut(s) 2970
BsaI GGTCTC 1 cut(s) 3762
BsaWI WCCGGW 4 cut(s) 1221, 1654, 3459, 5207
BsaXI ACNNNNNCTCC 4 cut(s) 1724, 1754, 4361, 4391
Bsc4I CCNNNNNNNGG 9 cut(s) 29, 85, 1173, 1319, 2006, 2148, 4174, 4564, 5794
Bse118I RCCGGY 1 cut(s) 1654
Bse1I ACTGG 7 cut(s) 1168, 2050, 3836, 4764, 4766, 5539, 5660
Bse21I CCTNAGG 1 cut(s) 675
Bse3DI GCAATG 5 cut(s) 2249, 2566, 3590, 4321, 5289
Bse8I GATNNNNATC 1 cut(s) 2970
BseAI TCCGGA 1 cut(s) 5207
BseCI ATCGAT 1 cut(s) 1327
BseGI GGATG 9 cut(s) 116, 709, 844, 2958, 3104, 3453, 4195, 4250, 5783
BseJI GATNNNNATC 1 cut(s) 2970
BseLI CCNNNNNNNGG 9 cut(s) 29, 85, 1173, 1319, 2006, 2148, 4174, 4564, 5794
BseMI GCAATG 5 cut(s) 2249, 2566, 3590, 4321, 5289
BseMII CTCAG 7 cut(s) 1712, 2700, 3674, 3773, 4186, 4438, 5391
BseNI ACTGG 7 cut(s) 1168, 2050, 3836, 4764, 4766, 5539, 5660
BseRI GAGGAG 5 cut(s) 2435, 2912, 3242, 3518, 5195
BseSI GKGCMC 4 cut(s) 424, 798, 850, 3673
BseYI CCCAGC 1 cut(s) 1790
BsgI GTGCAG 1 cut(s) 1263
Bsh1236I CGCG 3 cut(s) 1736, 2625, 3940
BshNI GGYRCC 3 cut(s) 109, 4047, 4832
BshTI ACCGGT 1 cut(s) 1654
BshVI ATCGAT 1 cut(s) 1327
BsiHKAI GWGCWC 7 cut(s) 424, 771, 798, 1916, 2767, 3673, 4938
BsiHKCI CYCGRG 3 cut(s) 80, 1453, 2938
BsiSI CCGG 8 cut(s) 23, 910, 1222, 1655, 3460, 5208, 5419, 5788
BslFI GGGAC 3 cut(s) 2314, 2687, 4804
BslI CCNNNNNNNGG 9 cut(s) 29, 85, 1173, 1319, 2006, 2148, 4174, 4564, 5794
BsmAI GTCTC 2 cut(s) 1889, 3762
BsmBI CGTCTC 1 cut(s) 1889
BsmFI GGGAC 3 cut(s) 2314, 2687, 4804
Bso31I GGTCTC 1 cut(s) 3762
BsoBI CYCGRG 3 cut(s) 80, 1453, 2938
Bsp1286I GDGCHC 9 cut(s) 424, 771, 798, 850, 1916, 2113, 2767, 3673, 4938
Bsp13I TCCGGA 1 cut(s) 5207
Bsp19I CCATGG 1 cut(s) 3093
Bsp68I TCGCGA 1 cut(s) 2625
BspCNI CTCAG 7 cut(s) 1711, 2701, 3675, 3772, 4185, 4437, 5392
BspDI ATCGAT 1 cut(s) 1327
BspEI TCCGGA 1 cut(s) 5207
BspFNI CGCG 3 cut(s) 1736, 2625, 3940
BspHI TCATGA 4 cut(s) 526, 2025, 3690, 5361
BspMAI CTGCAG 2 cut(s) 723, 5499
BspMI ACCTGC 1 cut(s) 3892
BspOI GCTAGC 1 cut(s) 2523
BspQI GCTCTTC 4 cut(s) 279, 651, 2772, 5720
BspT107I GGYRCC 3 cut(s) 109, 4047, 4832
BspTI CTTAAG 1 cut(s) 4028
BspTNI GGTCTC 1 cut(s) 3762
BsrBI CCGCTC 1 cut(s) 17
BsrDI GCAATG 5 cut(s) 2249, 2566, 3590, 4321, 5289
BsrFI RCCGGY 1 cut(s) 1654
BsrI ACTGG 7 cut(s) 1168, 2050, 3836, 4764, 4766, 5539, 5660
BssAI RCCGGY 1 cut(s) 1654
BssNAI GTATAC 1 cut(s) 1671
BssT1I CCWWGG 6 cut(s) 432, 668, 2203, 2550, 3093, 4254
Bst1107I GTATAC 1 cut(s) 1671
Bst6I CTCTTC 8 cut(s) 279, 651, 1071, 2772, 3293, 3351, 4632, 5720
BstAFI CTTAAG 1 cut(s) 4028
BstAPI GCANNNNNTGC 3 cut(s) 949, 2381, 4397
BstBAI YACGTR 2 cut(s) 341, 2036
BstC8I GCNNGC 9 cut(s) 962, 1242, 1788, 2521, 3953, 4288, 5546, 5566, 5645
BstDSI CCRYGG 4 cut(s) 2929, 3093, 5131, 5620
BstEII GGTNACC 3 cut(s) 927, 2735, 5032
BstENI CCTNNNNNAGG 1 cut(s) 83
BstF5I GGATG 9 cut(s) 116, 709, 844, 2958, 3104, 3453, 4195, 4250, 5783
BstFNI CGCG 3 cut(s) 1736, 2625, 3940
BstHHI GCGC 4 cut(s) 960, 1738, 2386, 3583
BstMAI GTCTC 2 cut(s) 1889, 3762
BstNSI RCATGY 5 cut(s) 1399, 2611, 2866, 3029, 5548
BstPI GGTNACC 3 cut(s) 927, 2735, 5032
BstSFI CTRYAG 5 cut(s) 719, 1843, 4848, 4894, 5495
BstSLI GKGCMC 4 cut(s) 424, 798, 850, 3673
BstSNI TACGTA 1 cut(s) 2036
BstUI CGCG 3 cut(s) 1736, 2625, 3940
BstV2I GAAGAC 4 cut(s) 656, 4281, 4534, 5372
BstX2I RGATCY 6 cut(s) 229, 2234, 2425, 2526, 4844, 4962
BstXI CCANNNNNNTGG 5 cut(s) 3318, 3638, 4808, 4967, 5538
BstYI RGATCY 6 cut(s) 229, 2234, 2425, 2526, 4844, 4962
BstZ17I GTATAC 1 cut(s) 1671
Bsu15I ATCGAT 1 cut(s) 1327
Bsu36I CCTNAGG 1 cut(s) 675
BsuI GTATCC 3 cut(s) 62, 2131, 5464
BsuTUI ATCGAT 1 cut(s) 1327
BtgI CCRYGG 4 cut(s) 2929, 3093, 5131, 5620
BtrI CACGTC 2 cut(s) 248, 3100
BtsCI GGATG 9 cut(s) 116, 709, 844, 2958, 3104, 3453, 4195, 4250, 5783
BtsIMutI CAGTG 8 cut(s) 106, 1175, 1215, 2057, 4521, 4539, 5007, 5401
BtuMI TCGCGA 1 cut(s) 2625
BveI ACCTGC 1 cut(s) 3892
Cac8I GCNNGC 9 cut(s) 962, 1242, 1788, 2521, 3953, 4288, 5546, 5566, 5645
CaiI CAGNNNCTG 4 cut(s) 1250, 1634, 3599, 3884
CciI TCATGA 4 cut(s) 526, 2025, 3690, 5361
CfoI GCGC 4 cut(s) 960, 1738, 2386, 3583
Cfr10I RCCGGY 1 cut(s) 1654
ClaI ATCGAT 1 cut(s) 1327
CseI GACGC 1 cut(s) 70
CsiI ACCWGGT 2 cut(s) 3852, 4563
Csp6I GTAC 9 cut(s) 1933, 2033, 3435, 3454, 3839, 4512, 4957, 5246, 5404
CspAI ACCGGT 1 cut(s) 1654
CviQI GTAC 9 cut(s) 1933, 2033, 3435, 3454, 3839, 4512, 4957, 5246, 5404
DraI TTTAAA 1 cut(s) 471
DriI GACNNNNNGTC 2 cut(s) 4146, 4425
EaeI YGGCCR 6 cut(s) 150, 1232, 2547, 3495, 4161, 5597
Eam1104I CTCTTC 8 cut(s) 279, 651, 1071, 2772, 3293, 3351, 4632, 5720
Eam1105I GACNNNNNGTC 2 cut(s) 4146, 4425
EarI CTCTTC 8 cut(s) 279, 651, 1071, 2772, 3293, 3351, 4632, 5720
EciI GGCGGA 3 cut(s) 1594, 5139, 5778
Ecl136II GAGCTC 1 cut(s) 4936
Eco105I TACGTA 1 cut(s) 2036
Eco130I CCWWGG 6 cut(s) 432, 668, 2203, 2550, 3093, 4254
Eco24I GRGCYC 2 cut(s) 2113, 4938
Eco31I GGTCTC 1 cut(s) 3762
Eco47I GGWCC 4 cut(s) 2009, 2488, 2674, 3881
Eco53kI GAGCTC 1 cut(s) 4936
Eco57I CTGAAG 4 cut(s) 2336, 3461, 3813, 4492
Eco72I CACGTG 1 cut(s) 341
Eco81I CCTNAGG 1 cut(s) 675
Eco88I CYCGRG 3 cut(s) 80, 1453, 2938
Eco91I GGTNACC 3 cut(s) 927, 2735, 5032
EcoICRI GAGCTC 1 cut(s) 4936
EcoNI CCTNNNNNAGG 1 cut(s) 83
EcoO109I RGGNCCY 1 cut(s) 3881
EcoO65I GGTNACC 3 cut(s) 927, 2735, 5032
EcoRI GAATTC 3 cut(s) 3145, 3541, 4736
EcoT14I CCWWGG 6 cut(s) 432, 668, 2203, 2550, 3093, 4254
EcoT22I ATGCAT 2 cut(s) 1908, 2613
EcoT38I GRGCYC 2 cut(s) 2113, 4938
ErhI CCWWGG 6 cut(s) 432, 668, 2203, 2550, 3093, 4254
Esp3I CGTCTC 1 cut(s) 1889
FalI AAGNNNNNCTT 8 cut(s) 3378, 3410, 4011, 4043, 4081, 4113, 5181, 5213
FaqI GGGAC 3 cut(s) 2314, 2687, 4804
FauI CCCGC 3 cut(s) 10, 2339, 4916
FauNDI CATATG 1 cut(s) 3616
FbaI TGATCA 5 cut(s) 820, 2965, 4518, 5004, 5424
FblI GTMKAC 1 cut(s) 1670
FokI GGATG 9 cut(s) 103, 716, 851, 2965, 3091, 3460, 4182, 4237, 5770
FriOI GRGCYC 2 cut(s) 2113, 4938
FspI TGCGCA 1 cut(s) 2385
GlaI GCGC 4 cut(s) 959, 1737, 2385, 3582
GsaI CCCAGC 1 cut(s) 1794
GsuI CTGGAG 3 cut(s) 2888, 3021, 3153
HapII CCGG 8 cut(s) 23, 910, 1222, 1655, 3460, 5208, 5419, 5788
HgaI GACGC 1 cut(s) 70
HhaI GCGC 4 cut(s) 960, 1738, 2386, 3583
Hin6I GCGC 4 cut(s) 958, 1736, 2384, 3581
HinP1I GCGC 4 cut(s) 958, 1736, 2384, 3581
HincII GTYRAC 2 cut(s) 1345, 2716
HindII GTYRAC 2 cut(s) 1345, 2716
HindIII AAGCTT 9 cut(s) 387, 869, 1384, 1497, 1532, 3465, 3872, 3993, 5562
HpaI GTTAAC 1 cut(s) 1345
HpaII CCGG 8 cut(s) 23, 910, 1222, 1655, 3460, 5208, 5419, 5788
HphI GGTGA 9 cut(s) 1082, 1767, 2747, 3671, 3956, 4214, 4480, 5350, 5647
Hpy99I CGWCG 4 cut(s) 10, 13, 128, 2202
HpyCH4IV ACGT 8 cut(s) 247, 283, 340, 1116, 1279, 2035, 3099, 5215
HpySE526I ACGT 8 cut(s) 247, 283, 340, 1116, 1279, 2035, 3099, 5215
HspAI GCGC 4 cut(s) 958, 1736, 2384, 3581
Kpn2I TCCGGA 1 cut(s) 5207
Ksp22I TGATCA 5 cut(s) 820, 2965, 4518, 5004, 5424
KspAI GTTAAC 1 cut(s) 1345
LguI GCTCTTC 4 cut(s) 279, 651, 2772, 5720
MabI ACCWGGT 2 cut(s) 3852, 4563
MaeII ACGT 8 cut(s) 247, 283, 340, 1116, 1279, 2035, 3099, 5215
MbiI CCGCTC 1 cut(s) 17
MfeI CAATTG 4 cut(s) 3819, 3960, 4494, 4751
MflI RGATCY 6 cut(s) 229, 2234, 2425, 2526, 4844, 4962
MhlI GDGCHC 9 cut(s) 424, 771, 798, 850, 1916, 2113, 2767, 3673, 4938
MlsI TGGCCA 5 cut(s) 152, 2549, 3497, 4163, 5599
MluI ACGCGT 1 cut(s) 3938
MluNI TGGCCA 5 cut(s) 152, 2549, 3497, 4163, 5599
MlyI GAGTC 4 cut(s) 1096, 3095, 3424, 5605
MmeI TCCRAC 5 cut(s) 207, 329, 816, 2036, 3132
Mox20I TGGCCA 5 cut(s) 152, 2549, 3497, 4163, 5599
Mph1103I ATGCAT 2 cut(s) 1908, 2613
MroI TCCGGA 1 cut(s) 5207
MroXI GAANNNNTTC 4 cut(s) 872, 3393, 4093, 5196
MscI TGGCCA 5 cut(s) 152, 2549, 3497, 4163, 5599
MslI CAYNNNNRTG 7 cut(s) 3098, 3617, 4200, 4340, 4806, 4830, 5549
Msp20I TGGCCA 5 cut(s) 152, 2549, 3497, 4163, 5599
MspA1I CMGCKG 5 cut(s) 46, 58, 71, 1631, 2285
MspCI CTTAAG 1 cut(s) 4028
MspI CCGG 8 cut(s) 23, 910, 1222, 1655, 3460, 5208, 5419, 5788
MunI CAATTG 4 cut(s) 3819, 3960, 4494, 4751
MvnI CGCG 3 cut(s) 1736, 2625, 3940
NciI CCSGG 1 cut(s) 5788
NcoI CCATGG 1 cut(s) 3093
NdeI CATATG 1 cut(s) 3616
NheI GCTAGC 1 cut(s) 2519
NmuCI GTSAC 8 cut(s) 1099, 1773, 1872, 1960, 2735, 2875, 4138, 5464
NruI TCGCGA 1 cut(s) 2625
NsbI TGCGCA 1 cut(s) 2385
NsiI ATGCAT 2 cut(s) 1908, 2613
NspI RCATGY 5 cut(s) 1399, 2611, 2866, 3029, 5548
OliI CACNNNNGTG 1 cut(s) 3617
PaeI GCATGC 1 cut(s) 5548
PagI TCATGA 4 cut(s) 526, 2025, 3690, 5361
PciI ACATGT 1 cut(s) 3025
PciSI GCTCTTC 4 cut(s) 279, 651, 2772, 5720
PcsI WCGNNNNNNNCGW 2 cut(s) 253, 5776
PdmI GAANNNNTTC 4 cut(s) 872, 3393, 4093, 5196
PflMI CCANNNNNTGG 1 cut(s) 1319
PfoI TCCNGGA 3 cut(s) 2856, 3877, 5786
PinAI ACCGGT 1 cut(s) 1654
PleI GAGTC 4 cut(s) 1096, 3094, 3424, 5605
PmaCI CACGTG 1 cut(s) 341
PmlI CACGTG 1 cut(s) 341
PpsI GAGTC 4 cut(s) 1096, 3094, 3424, 5605
Ppu21I YACGTR 2 cut(s) 341, 2036
PpuMI RGGWCCY 1 cut(s) 3881
PscI ACATGT 1 cut(s) 3025
PshBI ATTAAT 1 cut(s) 5435
PsiI TTATAA 3 cut(s) 1514, 2432, 2693
Psp124BI GAGCTC 1 cut(s) 4938
Psp5II RGGWCCY 1 cut(s) 3881
PspCI CACGTG 1 cut(s) 341
PspEI GGTNACC 3 cut(s) 927, 2735, 5032
PspFI CCCAGC 1 cut(s) 1790
PspPPI RGGWCCY 1 cut(s) 3881
PstI CTGCAG 2 cut(s) 723, 5499
PstNI CAGNNNCTG 4 cut(s) 1250, 1634, 3599, 3884
PsuI RGATCY 6 cut(s) 229, 2234, 2425, 2526, 4844, 4962
PvuII CAGCTG 2 cut(s) 71, 1631
RruI TCGCGA 1 cut(s) 2625
RsaI GTAC 9 cut(s) 1934, 2034, 3436, 3455, 3840, 4513, 4958, 5247, 5405
RsaNI GTAC 9 cut(s) 1933, 2033, 3435, 3454, 3839, 4512, 4957, 5246, 5404
RseI CAYNNNNRTG 7 cut(s) 3098, 3617, 4200, 4340, 4806, 4830, 5549
SacI GAGCTC 1 cut(s) 4938
SapI GCTCTTC 4 cut(s) 279, 651, 2772, 5720
SbfI CCTGCAGG 1 cut(s) 5499
ScaI AGTACT 1 cut(s) 4513
SchI GAGTC 4 cut(s) 1096, 3095, 3424, 5605
SdaI CCTGCAGG 1 cut(s) 5499
SduI GDGCHC 9 cut(s) 424, 771, 798, 850, 1916, 2113, 2767, 3673, 4938
SexAI ACCWGGT 2 cut(s) 3852, 4563
SfcI CTRYAG 5 cut(s) 719, 1843, 4848, 4894, 5495
SinI GGWCC 4 cut(s) 2009, 2488, 2674, 3881
SmiMI CAYNNNNRTG 7 cut(s) 3098, 3617, 4200, 4340, 4806, 4830, 5549
SmlI CTYRAG 4 cut(s) 1829, 3332, 4028, 4599
SmoI CTYRAG 4 cut(s) 1829, 3332, 4028, 4599
SnaBI TACGTA 1 cut(s) 2036
SphI GCATGC 1 cut(s) 5548
Sse8387I CCTGCAGG 1 cut(s) 5499
SspI AATATT 4 cut(s) 1473, 1527, 2172, 5439
SstI GAGCTC 1 cut(s) 4938
StyI CCWWGG 6 cut(s) 432, 668, 2203, 2550, 3093, 4254
TaiI ACGT 8 cut(s) 250, 286, 343, 1119, 1282, 2038, 3102, 5218
TaqII GACCGA 1 cut(s) 4352
TatI WGTACW 4 cut(s) 1932, 3453, 3838, 4511
TauI GCSGC 5 cut(s) 37, 40, 43, 49, 92
TscAI CASTG 8 cut(s) 106, 1175, 1215, 2057, 4528, 4539, 5014, 5401
TseFI GTSAC 8 cut(s) 1099, 1773, 1872, 1960, 2735, 2875, 4138, 5464
Tsp45I GTSAC 8 cut(s) 1099, 1773, 1872, 1960, 2735, 2875, 4138, 5464
TspGWI ACGGA 7 cut(s) 2186, 2230, 2735, 2946, 4295, 4457, 4828
TspRI CASTG 8 cut(s) 106, 1175, 1215, 2057, 4528, 4539, 5014, 5401
Van91I CCANNNNNTGG 1 cut(s) 1319
Vha464I CTTAAG 1 cut(s) 4028
VneI GTGCAC 3 cut(s) 420, 794, 3669
VpaK11BI GGWCC 4 cut(s) 2009, 2488, 2674, 3881
VspI ATTAAT 1 cut(s) 5435
XagI CCTNNNNNAGG 1 cut(s) 83
XbaI TCTAGA 1 cut(s) 1549
XceI RCATGY 5 cut(s) 1399, 2611, 2866, 3029, 5548
XcmI CCANNNNNNNNNTGG 3 cut(s) 145, 3250, 5311
XmiI GTMKAC 1 cut(s) 1670
XmnI GAANNNNTTC 4 cut(s) 872, 3393, 4093, 5196
ZrmI AGTACT 1 cut(s) 4513
Zsp2I ATGCAT 2 cut(s) 1908, 2613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.