MD03G1015000.v1.1

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005959
Physical Location & Seq
Reverse (-)
1187374 .. 1204387
17014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1015000.v1.1.491

Sequence Viewer

Length: 5880 bp
ATGGCGTCGTCGTCGAGAGGGGGTGGTTCGGAACAGCCACCTCAGCAGCCACTGCAGAGGCGGATAACCCGAACCCAGACCGCCGGGAACCTTGGGGAGACGGCATTCGACAGTGAGGTCGTGCCCTCTTCCCTTGTCGAAATCGCTCCCATTCTTCGTGTTGCCAATGAAGTCGAATCGCACAATCCTAGAGTTGCTTATCTCTGTCGATTTTATGCCTTTGAGAAGGCTCATAGGCTGGATCCCACTTCTAGTGGACGTGGCGTTCGTCAGTTTAAAACTGCCCTTCTTCAACGTCTTGAAAGGGAGAATGATCCAACACTCAAGGGAAGGGTTAAAAAAAGTGATGCACGTGAAATGCAGAGCTTCTACCATCAGTACTACAAAAAATATATTCAAGCTTTGTCAAATACTCATAAGGCTGACCGTGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTCTTTGAGGTTTTAAAGGCTGTTACAATGACACAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGATAAAGTTACAGAAAAGACACAGCTTTTGGTCCCTTACAATATCCTTCCACTTGATCCTGATAGTGCAAATCAAGCAATTATGAAATATCCAGAGGTACAAGCTGCTGTTTTTGCTCTTCGTAACACCAGGGGTCTTCCTCGGCCAAAGGATGACAATAAGAAAAACGAGGAAGACATTCTGGATTGGCTTCAGTCAATGTTTGGGTTTCAGAAGGATAATGTGGCAAATCAAAGGGAGCACCTTATACTTTTGCTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAACCTAAGTTGGATGATCGTGCATTGACAGAAGTGATGAAGAAGCTTTTCAAGAACTATAAAAAATGGTGCAAGTATTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACAATACAGCAAGAGGTGCAACAGCGCAAACTACTATACATGGGTCTATATCTTCTGATATGGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAGTGCCTTTGTTATATCTATCACCATATGGCATTTGAATTGTATGGTATGCTTGCTGGGAATGTCAGTCCAATGACGGGAGAGAACGTGAAGCCAGCCTATGGAGGCGAAGATGAGGCTTTCTTGAGAAAAGTTGTGACTCCAATTTATGAAGTGATCGCAAAAGAGGCTGAAAGGAGTAAACGAGGGAAATCAAAGCATTCCCAGTGGAGAAATTATGATGATTTAAATGAGTACTTTTGGTCAGTGGATTGTTTCCGGTTAGGCTGGCCAATGCGTGCAGGTGCTGATTTCTTTTGCATGCCTATCGATCAGCGCCATTTTGATAAAATTAATGAGGATAAAAAGCCAGCTAGTGGAGATCGATGGGTGGGGAAAGTTAATTTTGTTGAGATACGATCATTTTGGCATATTTTTAGAAGCTTTGACCGCATGTGGAGCTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAACGGCTCGGGGCAACCTACTGCATTATTTAATGGTGATGTATTCACGAAAGCATTGTCTGTCTTCATAACTGCTGCAGTATTGAAACTTGGGCAAGCGTTTCTAGATGTAATTCTTAGCTGGAAGGGTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATACATTTTGAAGGTCATCACAGCAGCTGGGTGGGTGGTAATTCTACCGATTACTTATGCATATAGTTGGAAAAACCCTCCTGCGTTTGCTCAAACTATCAAAAGTTGGTTTGGCAACGGTGGACATCAACCTTCTTTGTTTATCTTGGCTGTTGTTATCTACTTGTCACCAAACATGCTGGCTGCGGTGCTGTTTCTGTTCCCATTTATTCGTAGATTCCTTGAGAGATCAAATTATAAGATTGTGATGTTTATGATGTGGTGGTCACAGCCTCGACTTTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCCCTTTTCAAGTACACAATGTTTTGGGTACTTCTCATAATTACCAAGCTGGCATTCAGTTACTATATAGAGATAAAACCTTTAGTTGGTCCTACAAAAGCCATCATGAGTGTACGTGTAACCAATTTTCAGTGGCATGAGTTTTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTTGTGTATTTTATGGATACCCAGATTTGGTATGCCATTTTCTCCACAATATTTGGAGGAATTTATGGTGCATTCCGTCGTCTTGGAGAGATTCGAACATTAGGAATGCTGAGATCTCGTTTTCAATCACTTCCTGGTGCTTTTAATGCACGTTTGATTCCTGCGGAAAAGAGTCAACCAAGGAAGAAAGGACTGAAGGCTACTTTGTCTCGCAACTTTGCTCAGGTTGAAGTCAACAAAGAGAAAGAGGCGGCAAGATTTGCTCAATTATGGAACAAGATAATAAGTAGTTTCCGAGAGGAGGATCTTATAAACAATAGGGAGATGGACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGATTTAGGCCGCCTTACTCAGTGGCCTCCATTTTTACTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGTTGAAAAAGAGGATTGAGGCTGACAATTACATGTCTTGTGCTGTTTTGGAATGCTATGCTTCATTTAGGAACATAATCAAGCTTCTGGTTCAGGGAGAACGTGAGAAAGAGGTTATAGATTATATATTTTCTGAGGTTGAAAAGCGCATAGAGGTGGGTGACCTGATGGTTGAATACAAGATGAGTGCTCTTCCTAGCCTCTATGATCAATTCGTTAAGCTCATCAAACATCTGCTGGACAATAATAAGGACGAAAGGGATCAAGTTGTGATTCTGTTCCAGGACATGCTGGAGGTAGTGACTAGAGACATAATGATGGAGGACCATATATCCAGTTTGGTAGATTCAATCCATGGTGCCTCTGGCTATGAAGGGATGATGCCCCTTGATCAACCTCAACAGTATCAGTTGTTTGCGTCTTCTGGGGCTATCAGATTTCCTATTCAACAAGTAACAGAGGCGTGGAAGGAGAAGATTAAGCGTCTTGATCTTTTGCTTACGACAAAAGAGTCTGCCATGGATGTACCGTCCAACTTAGAAGCTAGAAGGCGGATTTCGTTCTTCTCAAATTCATTGTTTATGGACATGCCTCCATCACCAAAAGTTCGCAATATGCTCTCTTTCTCTGTTTTAACTCCTTACTACACCGAGGAGGTTCTGTTTTCATTGCGTGATTTGGAAGTGCCAAATGAAGATGGGGTTTCAATTCTCTTCTACTTGCAAAAAATTTACCCAGATGAGTGGAACAATTTTCTTCAGCGTGTCAATTGCACTAGTGAAGAGGAGCTTAAAGGATCAGATGAATTGGAAGAAGAACTTCGCCTTTGGGCATCATATAGAGGGCAAACTTTGACCCGTACTGTAAGGGGATTGATGTACTACCGGAAAGCTTTGGAGCTTCAGTCTTTTCTCGATATGGCCCAAGATGATGATTTAATGGAAGGCTATAAGGCCATAGAATTGAATTCTGAGGATCAATTAAAGGAAGGCAGGTCACTGTGGGCACAATGTCAAGCCGTAGCTGATTTGAAATTTACATATGTGGTATCATGCCAACTATATGGGATACACAAGCGATCTGGTGATCCTCGTGCGCAAGACATTCTGAGGCTTATGACAACATATCCATCACTACGTGTGGCATATATTGATGAGGTTGAGGAGCCTAGCAAAGATAGTTCCAAAAAGATGAACCAAAAAGTTTATTACTCTACTCTAGTCAAGGCTGCTCTACCAAAGTCAATTGATTCTTCAGAGCCCGTACAAAATCTGGACCAAGTAATTTATCGCATAAAGCTTCCAGGACCTGCTATTTTGGGTGAAGGAAAACCAGAGAATCAAAATCATGCCATTATTTTCACACGTGGAGAAGGTTTGCAAACAATTGATATGAACCAGGATAACTACATGGAAGAAGCCTTGAAGATGAGGAATTTGCTGCAAGAATTTCTCGAGAAGCATGATGGGGTGAGGCATCCTACAATTCTTGGACTTAGGGAGCATATATTTACTGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAAAACAGTTTTGTCACTATTGGTCAGAGACTGTTGGCCAATCCTCTCAAGGTTCGATTCCATTATGGTCATCCGGATGTGTTTGATAGGCTCTTTCACCTTACCAGAGGGGGTGTCAGTAAAGCATCCAAGGTTATCAATTTGAGTGAAGACATATTTGCTGGCTTTAATTCCACACTCCGTGAAGGCAATGTAACTCATCACGAGTACATACAAGTTGGGAAGGGAAGAGATGTGGGCCTTAACCAGATCTCTATGTTTGAAGCGAAAATAGCTAATGGAAATGGGGAGCAGACACTCAGTCGAGATATATACCGACTTGGACACCGTTTCGACTTTTTCCGAATGATGTCTTGCTATTTCACCACAATTGGTTTCTACTACAGTACTCTGATAACTGTGCTGACTGTGTACGTCTTCCTTTATGGACGCCTTTATCTGGTTCTTAGTGGACTTGAAGAGGGTTTGAGTACTCAACGAGCAATTCGAGACAACAAGCCTCTTCAAGTAGCTCTTGCGTCTCAAGCATTTGTTCAAATAGGATTTTTGATGGCGTTGCCTATGTTGATGGAAATTGGTTTGGAGAAGGGTTTCCGAACTGCACTAAGTGAATTCGTATTGATGCAATTGCAATTGGCCCCGGTATTTTTCACATTTTCACTTGGAACGAAGACCCACTATTATGGAAGGACATTACTTCATGGTGGTGCGAAATATAGATCTACAGGACGTGGGTTTGTGGTTTTCCATGCCAAATTTGCTGACAACTATAGGCTCTACTCCCGCAGCCACTTTGTTAAGGGCATTGAGCTCTTAATTTTACTTGTCGTGTACCAGATCTTTGGTCATACTTATAGAAGTGCTGTTGCGTACATTTTGATCACTGCATCTATGTGGTTTATGGTGTGTACCTGGCTCTTTGCTCCCTTTCTGTTCAATCCGTCTGGTTTTGAGTGGCAAAAGATTGTTGATGATTGGACGGATTGGAATAAGTGGATAAGCAACCGTGGGGGTATAGGTGTTCCACCTGAAAAAAGCTGGGAATCATGGTGGGAGGAGGAACAAGAGCATCTCCGATATTCTGGAAAACGTGGTATTGCAGCTGAGATACTGTTATCCTTACGGTTCTTTATCTATCAGTATGGGCTCGTATATCACTTGAACATTGCAAAGCATACCAAGAGTGTTATGGTCTATGGCATTTCGTGGCTGGTGATCGTCCTTATTTTGTTCGTTATGAAGACTGTATCTGTTGGGAGGCGAAAGTTCAGCGCAGATTTCCAACTTGTGTTCCGGCTGATCAAGGGACTAATATTCATAACTTTTGTGTCCATTTTGATCACTTTGATTGTGTTGCCTCACATGACCCTGCAGGATATCATCGTCTGCATTCTTGCTTTCATGCCAACTGGCTGGGGAATGCTTCTTATTGCACAAGCTTGCAAGCCTGTTGTGCACAGGGCTGGTTTATGGCCATCGGTTCGGACTCTTGCTCGCGGCTTTGAGATAGTTATGGGTCTCCTTCTATTCACCCCGGTTGCATTCTTGGCCTGGTTTCCCTTTGTTTCTGAATTCCAGACTCGTATGCTCTTCAACCAAGCATTCAGCAGAGGTTTGCAGATTTCTCGTATTCTTGGTGGGCAACGGAAGGATCGCTCATCCCGGAACAAGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1960

Amino Acids

226.27

Weight (kDa)

9.23

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 48 - 174 3.3e-15 Vta1 like
FKS1_dom1 PF14288 324 - 436 4.3e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 860 - 1052 5e-63 Callose synthase 1 domain
Glucan_synthase PF02364 1059 - 1149 1.9e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1152 - 1772 4.7e-210 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1917, 2504
AarI CACCTGC 1 cut(s) 1307
AasI GACNNNNNNGTC 2 cut(s) 116, 3160
Acc16I TGCGCA 1 cut(s) 3747
Acc36I ACCTGC 3 cut(s) 1307, 3633, 3967
AccB1I GGYRCC 1 cut(s) 3008
AccB7I CCANNNNNTGG 2 cut(s) 1136, 1391
AccII CGCG 1 cut(s) 5700
AccIII TCCGGA 1 cut(s) 4276
AcoI YGGCCR 5 cut(s) 680, 1304, 2622, 4239, 5675
AcuI CTGAAG 5 cut(s) 713, 2408, 3392, 3536, 3888
AcvI CACGTG 2 cut(s) 353, 4016
AcyI GRCGYC 2 cut(s) 5, 4633
AdeI CACNNNGTG 3 cut(s) 3788, 4385, 4811
AfiI CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 4642, 5872
AflIII ACRYGT 4 cut(s) 2107, 2682, 3787, 4013
AhdI GACNNNNNGTC 1 cut(s) 4503
AhlI ACTAGT 1 cut(s) 3425
AjiI CACGTC 2 cut(s) 260, 4934
AjnI CCWGG 7 cut(s) 665, 2326, 2931, 3952, 4047, 5114, 5753
AjuI GAANNNNNNNTTGG 2 cut(s) 2030, 2062
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
Alw21I GWGCWC 7 cut(s) 433, 780, 807, 1988, 2842, 5016, 5661
Alw26I GTCTC 7 cut(s) 92, 2406, 2952, 4225, 4686, 4728, 5726
Alw44I GTGCAC 3 cut(s) 429, 803, 5657
AlwNI CAGNNNCTG 5 cut(s) 52, 1322, 1706, 3959, 4234
Ama87I CYCGRG 2 cut(s) 1525, 4103
Aor13HI TCCGGA 1 cut(s) 4276
ApaLI GTGCAC 3 cut(s) 429, 803, 5657
ArsI GACNNNNNNTTYG 6 cut(s) 249, 281, 547, 579, 1559, 1591
AseI ATTAAT 1 cut(s) 1368
Asp700I GAANNNNTTC 5 cut(s) 714, 881, 3468, 4171, 4793
AspLEI GCGC 5 cut(s) 969, 1353, 2799, 3748, 5477
AsuC2I CCSGG 4 cut(s) 85, 4844, 5738, 5866
AsuII TTCGAA 1 cut(s) 2287
AsuNHI GCTAGC 1 cut(s) 2594
AvaI CYCGRG 2 cut(s) 1525, 4103
AvaII GGWCC 6 cut(s) 568, 2081, 2521, 2974, 3925, 3956
BaeGI GKGCMC 5 cut(s) 126, 433, 807, 3658, 5661
BalI TGGCCA 4 cut(s) 1306, 2624, 4241, 5677
BamHI GGATCC 1 cut(s) 241
BanI GGYRCC 1 cut(s) 3008
BanII GRGCYC 4 cut(s) 2185, 3912, 5016, 5352
BauI CACGAG 2 cut(s) 3741, 4406
BbrPI CACGTG 2 cut(s) 353, 4016
BbsI GAAGAC 8 cut(s) 665, 717, 1573, 3063, 4359, 4612, 4880, 5450
Bbv12I GWGCWC 7 cut(s) 433, 780, 807, 1988, 2842, 5016, 5661
BbvCI CCTCAGC 1 cut(s) 42
BceAI ACGGC 3 cut(s) 117, 1537, 3653
BcgI CGANNNNNNTGC 4 cut(s) 1324, 1358, 5810, 5844
BciT130I CCWGG 7 cut(s) 667, 2328, 2933, 3954, 4049, 5116, 5755
BciVI GTATCC 2 cut(s) 2203, 3711
BclI TGATCA 6 cut(s) 829, 2857, 3040, 5082, 5502, 5541
BcnI CCSGG 4 cut(s) 85, 4844, 5738, 5866
BcoDI GTCTC 7 cut(s) 92, 2406, 2952, 4225, 4686, 4728, 5726
BcuI ACTAGT 1 cut(s) 3425
BfmI CTRYAG 6 cut(s) 53, 1593, 4585, 4926, 4972, 5573
BfoI RGCGCY 1 cut(s) 1354
BfuAI ACCTGC 3 cut(s) 1307, 3633, 3967
BfuI GTATCC 2 cut(s) 2203, 3711
BglII AGATCT 4 cut(s) 2306, 4452, 4922, 5040
BmcAI AGTACT 4 cut(s) 380, 1271, 4591, 4675
Bme18I GGWCC 6 cut(s) 568, 2081, 2521, 2974, 3925, 3956
BmeRI GACNNNNNGTC 1 cut(s) 4503
BmeT110I CYCGRG 2 cut(s) 1525, 4103
BmgBI CACGTC 2 cut(s) 260, 4934
BmiI GGNNCC 7 cut(s) 89, 243, 570, 2184, 3010, 3816, 4842
BmrI ACTGGG 1 cut(s) 1234
BmsI GCATC 8 cut(s) 337, 3021, 3491, 4135, 4337, 4815, 5099, 5281
BmtI GCTAGC 1 cut(s) 2598
BmuI ACTGGG 1 cut(s) 1234
BpiI GAAGAC 8 cut(s) 665, 717, 1573, 3063, 4359, 4612, 4880, 5450
BpmI CTGGAG 1 cut(s) 2963
Bpu10I CCTNAGC 2 cut(s) 42, 2415
Bpu14I TTCGAA 1 cut(s) 2287
BpuEI CTTGAG 5 cut(s) 308, 1180, 1922, 4235, 4710
BpuMI CCSGG 4 cut(s) 85, 4844, 5738, 5866
Bsa29I ATCGAT 2 cut(s) 1344, 1399
BsaAI YACGTR 4 cut(s) 353, 2108, 3788, 4016
BsaHI GRCGYC 2 cut(s) 5, 4633
BsaI GGTCTC 1 cut(s) 5726
BsaWI WCCGGW 3 cut(s) 1293, 3534, 4276
BsaXI ACNNNNNCTCC 6 cut(s) 1107, 1137, 3296, 3326, 3539, 3569
Bsc4I CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 4642, 5872
Bse1I ACTGG 4 cut(s) 1240, 2985, 4168, 5617
Bse3DI GCAATG 4 cut(s) 2641, 3317, 4399, 5367
BseAI TCCGGA 1 cut(s) 4276
BseBI CCWGG 7 cut(s) 667, 2328, 2933, 3954, 4049, 5116, 5755
BseCI ATCGAT 2 cut(s) 1344, 1399
BseGI GGATG 9 cut(s) 694, 853, 3033, 3178, 4126, 4273, 4285, 4328, 5861
BseLI CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 4642, 5872
BseMI GCAATG 4 cut(s) 2641, 3317, 4399, 5367
BseMII CTCAG 9 cut(s) 56, 2294, 2429, 2585, 2775, 3612, 3749, 4516, 5298
BseNI ACTGG 4 cut(s) 1240, 2985, 4168, 5617
BseRI GAGGAG 5 cut(s) 2507, 3317, 3449, 3827, 5273
BseSI GKGCMC 5 cut(s) 126, 433, 807, 3658, 5661
BseYI CCCAGC 4 cut(s) 1091, 1706, 5241, 5616
BsgI GTGCAG 2 cut(s) 1335, 4788
Bsh1236I CGCG 1 cut(s) 5700
BshNI GGYRCC 1 cut(s) 3008
BshVI ATCGAT 2 cut(s) 1344, 1399
BsiHKAI GWGCWC 7 cut(s) 433, 780, 807, 1988, 2842, 5016, 5661
BsiHKCI CYCGRG 2 cut(s) 1525, 4103
BsiSI CCGG 9 cut(s) 84, 919, 1294, 3535, 4277, 4844, 5497, 5738, 5866
BslFI GGGAC 2 cut(s) 554, 5523
BslI CCNNNNNNNGG 8 cut(s) 1112, 1136, 1391, 2078, 2220, 2494, 4642, 5872
BsmAI GTCTC 7 cut(s) 92, 2406, 2952, 4225, 4686, 4728, 5726
BsmBI CGTCTC 2 cut(s) 92, 4728
BsmFI GGGAC 2 cut(s) 554, 5523
Bso31I GGTCTC 1 cut(s) 5726
BsoBI CYCGRG 2 cut(s) 1525, 4103
Bsp119I TTCGAA 1 cut(s) 2287
Bsp13I TCCGGA 1 cut(s) 4276
Bsp19I CCATGG 2 cut(s) 3004, 3168
BspCNI CTCAG 9 cut(s) 55, 2295, 2428, 2584, 2776, 3613, 3750, 4515, 5299
BspDI ATCGAT 2 cut(s) 1344, 1399
BspEI TCCGGA 1 cut(s) 4276
BspFNI CGCG 1 cut(s) 5700
BspHI TCATGA 2 cut(s) 535, 2097
BspLI GGNNCC 7 cut(s) 89, 243, 570, 2184, 3010, 3816, 4842
BspMAI CTGCAG 3 cut(s) 57, 1597, 5577
BspMI ACCTGC 3 cut(s) 1307, 3633, 3967
BspOI GCTAGC 1 cut(s) 2598
BspQI GCTCTTC 3 cut(s) 660, 2847, 5798
BspT104I TTCGAA 1 cut(s) 2287
BspT107I GGYRCC 1 cut(s) 3008
BspTNI GGTCTC 1 cut(s) 5726
BsrDI GCAATG 4 cut(s) 2641, 3317, 4399, 5367
BsrI ACTGG 4 cut(s) 1240, 2985, 4168, 5617
BssNI GRCGYC 2 cut(s) 5, 4633
BssSI CACGAG 2 cut(s) 3741, 4406
BssT1I CCWWGG 7 cut(s) 91, 441, 2372, 2625, 3004, 3168, 4332
Bst2BI CACGAG 2 cut(s) 3741, 4406
Bst2UI CCWGG 7 cut(s) 667, 2328, 2933, 3954, 4049, 5116, 5755
Bst6I CTCTTC 9 cut(s) 133, 660, 2847, 3368, 3426, 4426, 4656, 4710, 5798
BstACI GRCGYC 2 cut(s) 5, 4633
BstAPI GCANNNNNTGC 3 cut(s) 52, 958, 2453
BstBAI YACGTR 4 cut(s) 353, 2108, 3788, 4016
BstBI TTCGAA 1 cut(s) 2287
BstDSI CCRYGG 3 cut(s) 3004, 3168, 5209
BstEII GGTNACC 2 cut(s) 936, 2810
BstF5I GGATG 9 cut(s) 694, 853, 3033, 3178, 4126, 4273, 4285, 4328, 5861
BstFNI CGCG 1 cut(s) 5700
BstH2I RGCGCY 1 cut(s) 1354
BstHHI GCGC 5 cut(s) 969, 1353, 2799, 3748, 5477
BstMAI GTCTC 7 cut(s) 92, 2406, 2952, 4225, 4686, 4728, 5726
BstNI CCWGG 7 cut(s) 667, 2328, 2933, 3954, 4049, 5116, 5755
BstNSI RCATGY 6 cut(s) 1339, 1471, 1858, 2686, 2941, 3241
BstPI GGTNACC 2 cut(s) 936, 2810
BstSFI CTRYAG 6 cut(s) 53, 1593, 4585, 4926, 4972, 5573
BstSLI GKGCMC 5 cut(s) 126, 433, 807, 3658, 5661
BstUI CGCG 1 cut(s) 5700
BstV2I GAAGAC 8 cut(s) 665, 717, 1573, 3063, 4359, 4612, 4880, 5450
BstX2I RGATCY 7 cut(s) 241, 2306, 2497, 2601, 4452, 4922, 5040
BstXI CCANNNNNNTGG 5 cut(s) 3393, 3713, 4886, 5045, 5616
BstYI RGATCY 7 cut(s) 241, 2306, 2497, 2601, 4452, 4922, 5040
Bsu15I ATCGAT 2 cut(s) 1344, 1399
BsuI GTATCC 2 cut(s) 2203, 3711
BsuTUI ATCGAT 2 cut(s) 1344, 1399
BtgI CCRYGG 3 cut(s) 3004, 3168, 5209
BtrI CACGTC 2 cut(s) 260, 4934
BtsCI GGATG 9 cut(s) 694, 853, 3033, 3178, 4126, 4273, 4285, 4328, 5861
BtsI GCAGTG 2 cut(s) 50, 5085
BtsIMutI CAGTG 8 cut(s) 50, 118, 1247, 1287, 2129, 2579, 3647, 5085
BveI ACCTGC 3 cut(s) 1307, 3633, 3967
CaiI CAGNNNCTG 5 cut(s) 52, 1322, 1706, 3959, 4234
CciI TCATGA 2 cut(s) 535, 2097
CfoI GCGC 5 cut(s) 969, 1353, 2799, 3748, 5477
ClaI ATCGAT 2 cut(s) 1344, 1399
CseI GACGC 4 cut(s) 3057, 3122, 4641, 4710
CspCI CAANNNNNGTGG 2 cut(s) 2227, 2262
DraI TTTAAA 3 cut(s) 277, 480, 1263
DraIII CACNNNGTG 3 cut(s) 3788, 4385, 4811
DrdI GACNNNNNNGTC 2 cut(s) 116, 3160
DriI GACNNNNNGTC 1 cut(s) 4503
DseDI GACNNNNNNGTC 2 cut(s) 116, 3160
EaeI YGGCCR 5 cut(s) 680, 1304, 2622, 4239, 5675
Eam1104I CTCTTC 9 cut(s) 133, 660, 2847, 3368, 3426, 4426, 4656, 4710, 5798
Eam1105I GACNNNNNGTC 1 cut(s) 4503
EarI CTCTTC 9 cut(s) 133, 660, 2847, 3368, 3426, 4426, 4656, 4710, 5798
EciI GGCGGA 3 cut(s) 76, 1666, 3217
Ecl136II GAGCTC 1 cut(s) 5014
Eco130I CCWWGG 7 cut(s) 91, 441, 2372, 2625, 3004, 3168, 4332
Eco24I GRGCYC 4 cut(s) 2185, 3912, 5016, 5352
Eco31I GGTCTC 1 cut(s) 5726
Eco32I GATATC 1 cut(s) 5581
Eco47I GGWCC 6 cut(s) 568, 2081, 2521, 2974, 3925, 3956
Eco53kI GAGCTC 1 cut(s) 5014
Eco57I CTGAAG 5 cut(s) 713, 2408, 3392, 3536, 3888
Eco72I CACGTG 2 cut(s) 353, 4016
Eco88I CYCGRG 2 cut(s) 1525, 4103
Eco91I GGTNACC 2 cut(s) 936, 2810
EcoICRI GAGCTC 1 cut(s) 5014
EcoO109I RGGNCCY 1 cut(s) 3956
EcoO65I GGTNACC 2 cut(s) 936, 2810
EcoRI GAATTC 3 cut(s) 3616, 4814, 5774
EcoRII CCWGG 7 cut(s) 665, 2326, 2931, 3952, 4047, 5114, 5753
EcoRV GATATC 1 cut(s) 5581
EcoT14I CCWWGG 7 cut(s) 91, 441, 2372, 2625, 3004, 3168, 4332
EcoT22I ATGCAT 2 cut(s) 1741, 1980
EcoT38I GRGCYC 4 cut(s) 2185, 3912, 5016, 5352
ErhI CCWWGG 7 cut(s) 91, 441, 2372, 2625, 3004, 3168, 4332
Esp3I CGTCTC 2 cut(s) 92, 4728
FalI AAGNNNNNCTT 8 cut(s) 3423, 3455, 3453, 3485, 4159, 4191, 4701, 4733
FaqI GGGAC 2 cut(s) 554, 5523
FauI CCCGC 1 cut(s) 4994
FauNDI CATATG 2 cut(s) 1062, 3691
FbaI TGATCA 6 cut(s) 829, 2857, 3040, 5082, 5502, 5541
FokI GGATG 9 cut(s) 701, 860, 3040, 3185, 4113, 4260, 4292, 4315, 5848
FriOI GRGCYC 4 cut(s) 2185, 3912, 5016, 5352
FspI TGCGCA 1 cut(s) 3747
GlaI GCGC 5 cut(s) 968, 1352, 2798, 3747, 5476
GsaI CCCAGC 4 cut(s) 1095, 1710, 5245, 5620
GsuI CTGGAG 1 cut(s) 2963
HaeII RGCGCY 1 cut(s) 1354
HapII CCGG 9 cut(s) 84, 919, 1294, 3535, 4277, 4844, 5497, 5738, 5866
HgaI GACGC 4 cut(s) 3057, 3122, 4641, 4710
HhaI GCGC 5 cut(s) 969, 1353, 2799, 3748, 5477
Hin1I GRCGYC 2 cut(s) 5, 4633
Hin6I GCGC 5 cut(s) 967, 1351, 2797, 3746, 5475
HinP1I GCGC 5 cut(s) 967, 1351, 2797, 3746, 5475
HincII GTYRAC 2 cut(s) 2369, 2428
HindII GTYRAC 2 cut(s) 2369, 2428
HindIII AAGCTT 7 cut(s) 399, 878, 1456, 2732, 3540, 3947, 5640
HpaII CCGG 9 cut(s) 84, 919, 1294, 3535, 4277, 4844, 5497, 5738, 5866
Hpy99I CGWCG 4 cut(s) 10, 13, 16, 2274
Hsp92I GRCGYC 2 cut(s) 5, 4633
HspAI GCGC 5 cut(s) 967, 1351, 2797, 3746, 5475
Kpn2I TCCGGA 1 cut(s) 4276
Ksp22I TGATCA 6 cut(s) 829, 2857, 3040, 5082, 5502, 5541
LguI GCTCTTC 3 cut(s) 660, 2847, 5798
LweI GCATC 8 cut(s) 337, 3021, 3491, 4135, 4337, 4815, 5099, 5281
MfeI CAATTG 6 cut(s) 3418, 3894, 4035, 4572, 4829, 4835
MflI RGATCY 7 cut(s) 241, 2306, 2497, 2601, 4452, 4922, 5040
MlsI TGGCCA 4 cut(s) 1306, 2624, 4241, 5677
MluNI TGGCCA 4 cut(s) 1306, 2624, 4241, 5677
MlyI GAGTC 5 cut(s) 1168, 2374, 3170, 5683, 5776
MmeI TCCRAC 5 cut(s) 341, 825, 1727, 3207, 5509
Mox20I TGGCCA 4 cut(s) 1306, 2624, 4241, 5677
Mph1103I ATGCAT 2 cut(s) 1741, 1980
MroI TCCGGA 1 cut(s) 4276
MroXI GAANNNNTTC 5 cut(s) 714, 881, 3468, 4171, 4793
MscI TGGCCA 4 cut(s) 1306, 2624, 4241, 5677
MslI CAYNNNNRTG 6 cut(s) 2804, 2966, 4278, 4884, 4908, 5095
Msp20I TGGCCA 4 cut(s) 1306, 2624, 4241, 5677
MspA1I CMGCKG 2 cut(s) 1706, 5306
MspI CCGG 9 cut(s) 84, 919, 1294, 3535, 4277, 4844, 5497, 5738, 5866
MunI CAATTG 6 cut(s) 3418, 3894, 4035, 4572, 4829, 4835
MvaI CCWGG 7 cut(s) 667, 2328, 2933, 3954, 4049, 5116, 5755
MvnI CGCG 1 cut(s) 5700
NciI CCSGG 4 cut(s) 85, 4844, 5738, 5866
NcoI CCATGG 2 cut(s) 3004, 3168
NdeI CATATG 2 cut(s) 1062, 3691
NheI GCTAGC 1 cut(s) 2594
NlaIV GGNNCC 7 cut(s) 89, 243, 570, 2184, 3010, 3816, 4842
NmeAIII GCCGAG 1 cut(s) 658
NmuCI GTSAC 7 cut(s) 1171, 1845, 1944, 2810, 2950, 3645, 4216
NsbI TGCGCA 1 cut(s) 3747
NsiI ATGCAT 2 cut(s) 1741, 1980
NspI RCATGY 6 cut(s) 1339, 1471, 1858, 2686, 2941, 3241
NspV TTCGAA 1 cut(s) 2287
PaeI GCATGC 1 cut(s) 1339
PaeR7I CTCGAG 1 cut(s) 4103
PagI TCATGA 2 cut(s) 535, 2097
PaqCI CACCTGC 1 cut(s) 1307
PciI ACATGT 1 cut(s) 2682
PciSI GCTCTTC 3 cut(s) 660, 2847, 5798
PcsI WCGNNNNNNNCGW 2 cut(s) 265, 4687
PdmI GAANNNNTTC 5 cut(s) 714, 881, 3468, 4171, 4793
PflMI CCANNNNNTGG 2 cut(s) 1136, 1391
PfoI TCCNGGA 3 cut(s) 2931, 3952, 5864
PleI GAGTC 5 cut(s) 1168, 2373, 3169, 5683, 5776
PmaCI CACGTG 2 cut(s) 353, 4016
PmlI CACGTG 2 cut(s) 353, 4016
PpsI GAGTC 5 cut(s) 1168, 2373, 3169, 5683, 5776
Ppu21I YACGTR 4 cut(s) 353, 2108, 3788, 4016
PpuMI RGGWCCY 1 cut(s) 3956
PscI ACATGT 1 cut(s) 2682
PshBI ATTAAT 1 cut(s) 1368
PsiI TTATAA 2 cut(s) 1917, 2504
Psp124BI GAGCTC 1 cut(s) 5016
Psp5II RGGWCCY 1 cut(s) 3956
Psp6I CCWGG 7 cut(s) 665, 2326, 2931, 3952, 4047, 5114, 5753
PspCI CACGTG 2 cut(s) 353, 4016
PspEI GGTNACC 2 cut(s) 936, 2810
PspFI CCCAGC 4 cut(s) 1091, 1706, 5241, 5616
PspGI CCWGG 7 cut(s) 665, 2326, 2931, 3952, 4047, 5114, 5753
PspN4I GGNNCC 7 cut(s) 89, 243, 570, 2184, 3010, 3816, 4842
PspPPI RGGWCCY 1 cut(s) 3956
PstI CTGCAG 3 cut(s) 57, 1597, 5577
PstNI CAGNNNCTG 5 cut(s) 52, 1322, 1706, 3959, 4234
PsuI RGATCY 7 cut(s) 241, 2306, 2497, 2601, 4452, 4922, 5040
PvuII CAGCTG 2 cut(s) 1706, 5306
RseI CAYNNNNRTG 6 cut(s) 2804, 2966, 4278, 4884, 4908, 5095
SacI GAGCTC 1 cut(s) 5016
SapI GCTCTTC 3 cut(s) 660, 2847, 5798
SbfI CCTGCAGG 1 cut(s) 5577
ScaI AGTACT 4 cut(s) 380, 1271, 4591, 4675
SchI GAGTC 5 cut(s) 1168, 2374, 3170, 5683, 5776
SdaI CCTGCAGG 1 cut(s) 5577
SfaNI GCATC 8 cut(s) 337, 3021, 3491, 4135, 4337, 4815, 5099, 5281
SfcI CTRYAG 6 cut(s) 53, 1593, 4585, 4926, 4972, 5573
Sfr274I CTCGAG 1 cut(s) 4103
SfuI TTCGAA 1 cut(s) 2287
SinI GGWCC 6 cut(s) 568, 2081, 2521, 2974, 3925, 3956
SlaI CTCGAG 1 cut(s) 4103
SmiI ATTTAAAT 1 cut(s) 1263
SmiMI CAYNNNNRTG 6 cut(s) 2804, 2966, 4278, 4884, 4908, 5095
SmlI CTYRAG 6 cut(s) 323, 1159, 1901, 4103, 4250, 4725
SmoI CTYRAG 6 cut(s) 323, 1159, 1901, 4103, 4250, 4725
SpeI ACTAGT 1 cut(s) 3425
SphI GCATGC 1 cut(s) 1339
Sse8387I CCTGCAGG 1 cut(s) 5577
SspI AATATT 2 cut(s) 2244, 5517
SstI GAGCTC 1 cut(s) 5016
StyI CCWWGG 7 cut(s) 91, 441, 2372, 2625, 3004, 3168, 4332
SwaI ATTTAAAT 1 cut(s) 1263
TatI WGTACW 7 cut(s) 378, 1269, 2004, 3528, 4410, 4589, 4673
TauI GCSGC 3 cut(s) 2447, 2565, 5703
TscAI CASTG 8 cut(s) 57, 118, 1247, 1287, 2129, 2579, 3654, 5092
TseFI GTSAC 7 cut(s) 1171, 1845, 1944, 2810, 2950, 3645, 4216
Tsp45I GTSAC 7 cut(s) 1171, 1845, 1944, 2810, 2950, 3645, 4216
TspGWI ACGGA 5 cut(s) 2258, 4373, 5133, 5198, 5863
TspRI CASTG 8 cut(s) 57, 118, 1247, 1287, 2129, 2579, 3654, 5092
Van91I CCANNNNNTGG 2 cut(s) 1136, 1391
VneI GTGCAC 3 cut(s) 429, 803, 5657
VpaK11BI GGWCC 6 cut(s) 568, 2081, 2521, 2974, 3925, 3956
VspI ATTAAT 1 cut(s) 1368
XbaI TCTAGA 1 cut(s) 1621
XceI RCATGY 6 cut(s) 1339, 1471, 1858, 2686, 2941, 3241
XcmI CCANNNNNNNNNTGG 2 cut(s) 3011, 5389
XhoI CTCGAG 1 cut(s) 4103
XmnI GAANNNNTTC 5 cut(s) 714, 881, 3468, 4171, 4793
ZrmI AGTACT 4 cut(s) 380, 1271, 4591, 4675
Zsp2I ATGCAT 2 cut(s) 1741, 1980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.