RLG00000036880

Callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005959
Physical Location & Seq
Forward (+)
86078632 .. 86092797
14166 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036880

Sequence Viewer

Length: 5553 bp
ATGGGAAGGGTAAAAAAAAGTGATGCACGCGAAATGCAGAGCTTCTATCAACACTATTACAAAAAATATATTCAAGCTTTGCAAAATGCGGCCGATAAAGCTGACCGTGCACAACTTACCAAGGCATACCAGACTGCCAATGTTCTATTTGAGGTTTTAAAGGCTGTTAATATGACACAATCTATGGAAGTTGATCGTGAGATTTTGGAAGCTCATGATAAAGTTGCAGAAAAGACAGAGCTATTGGTTCCTTACAATATCCTTCCACTTGATCCTGATAGTGTAAATCAAGCAATTATGAGATACCCAGAGATTCAAGCTGCTGTTTTTGCTCTTCGTAACACCAGGGGTCTTCCTTGGCCTAAGGAATACAAAAAGAAAAAAGACGAGGATGTTCTTGATTGGCTGCAGTCTATGTTTGGGTTTCAGAAGGACAATGTGGCGAATCAACGGGAGCACCTGATATTGTTACTTGCAAATGTGCACATAAGGCAGTTTCCAAAGCCTGATCAACAGCCCAAGTTGGATGACCGTGCCCTGACAGAAGTGATGAAGAAGCTTTTCAAGAATTACAAAAAATGGTGCAAGTTCTTGGGCCGGAAAAGTAGCCTTTGGTTACCAACCATACAGCAAGAGGTGCAACAGCGCAAGCTGCTTTACATGGGTCTATATCTTTTGATATGGGGGGAAGCTGCAAATTTGAGATTCATGCCAGAATGCCTTTGTTACATTTATCATCATATGGCATTTGAATTGTATGGTATGTTAGCTGGGAATGTGAGTCCAATGACAGGAGAAAATGTGAAGCCAGCCTATGGAGGTGAAGAAGAGGCTTTCTTGAAAAAAGTTGTGACTCCTATCTATAACGTGATTGCCAGGGAAGCTGATAGGAGCAAAAGAGGGGAATCAAAGCATTCCCAGTGGAGGAACTATGATGATATAAATGAGTACTTTTGGTCAGTGGATTGTTTCCGGTTGGGTTGGCCGATGCGTGCAGATGCTGATTTCTTTTGCTGCTCTAGTGAACAACGTCGATTTGATAAAAATAGTGAGGATAACAAACCAGCTAGTGCAGATCGATGGGTGGGGAAAGTTAACTTCGTTGAGATACGGTCATTCTGGCATATCTTTAGAAGCTTTGACCGCATGTGGAGTTTCTTTATTCTATGTTTACAGGTTATGATAATTGTTGCTTGGAATGGCTCGGGCCAACCCACATCAATATTTTCTGCCGATGTATTCAAGAAAGCTTTGAGTGTCTTTATAACTGCTGCAATATTGAAGCTTGGACAAGCTGTTCTAGATGTAATTCTTAGCTGGAAGTCTAGGCGGAGTATGTCATTCCATGTTAAGTTGAGATATATTGCTAAGGTCATTTCAGCTGCTGCGTGGGTAATAATTCTACCGGTTACTTATGCGTATACTTGGGAAAACCCTCCTGGGTTTGCTCAGACCATTAAAGGTTGGTTTGGCAATAGTAAAAATGCGCCCTCCTTGTTCATCTTGGCTGTTGTTGTCTACTTGTCACCGAATATGCTGGCTGGGGTGTTGTTTCTTTTCCCATTTATTCGCCGATTCCTTGAGAGATCAAACTATAGGATTGTGATGCTTATGATGTGGTGGTCACAGCCTCGTCTCTATGTTGGGAGGGGAATGCATGAGAGCACATTTTCTCTTTTCAAGTACACGATGTTTTGGGTTCTCCTTGTAGTCACAAAGTTGGCATTCAGTTACTATATAGAGATAAAGCCTTTAGTGGGTCCAACAAAAGCCATCATGAGTGTACGTATAACTAATTTCCAGTGGCATGAGTTCTTTCCCCGTGCAAAGAACAATATCGGTGTTGTGATTGCACTCTGGGCTCCAATTATCCTGGTCTATTTTATGGATACCCAGATTTGGTATGCAATATACTCCACAATATTCGGAGGAATTTATGGTGCATTCCGTCGCCTTGGAGAGATACGGACACTAGGAATGCTAAGATCTCGCTTTGAATCATTGCCTGGTGCTTTTAATGCCCGTTTAATTCCAGCGGACAAGAGTGCGCCAAAGAAGAAAGGACTGAAGGCTACATTGTCCCGCAGCTTTGGTCATGTTGAAGGTAGTAAAGAGAATCAGGCTGCAAGGTTTGCGCAATTATGGAACAAAATAATAAGTAGTTTCAGAGAGGAGGATCTTATAAACAATAGGGAAATGGACCTTTTGCTTGTTCCTTATTGGGCTGATCGTGAATTAAACCTCATACAGTGGCCTCCATTTTTACTTGCTAGCAAGATCCCGATAGCATTAGACATGGCCAAGGACAGCAATGGCAAGGATAAAGAGCTAACAAAAAGAATCCTGGCTGATGAATACATGCATTGTGCTGTTCGTGAATGCTATGCTTCATTTAAGAACATAATTTTGTTTCTGGTTCAGGGGGACCGTGAGAAAGATGTTATAAATTTCATATTTTCTGAGGTTGACAAGCACATAAAAGATGGTGACCTGATCCGTGAATTCAAGATGAGTGCTCTTCCTAGCCTCTATGACCACTTTGTTAGCCTTATCAAATTTTTGTTAAAGAATAGTCAGGACGACAGGGATCAAGTTGTGATTCTATTCCAGGACATGCTGGAGGTAGTGACTAGAGATATAATGATGGAGGACCATATAAACAGCTTGGTAGATTCAGTCCACGGAGGCTCGGGCCATGAGGGGATGATGCCCCTTGATCAACATCAACAGCATCAGTTGTTTGCATCTGCTGGAGCTATCAACTTTCCTCTTACACATGTAACAGAGGCTTGGAAGGAGAAGATTAACCGTCTTTATCTATTACTTACCACAAAGGAGTCTGCCATGGACGTGCCATCCAACTTAGAAGCTAGAAGGCGAATTTCTTTCTTCTCGAATTCATTGTTTATGGATATGCCTCCAGCACCAAAAGTTCGCAATATGCTTTCTTTCTCTGTTTTAACTCCTTACTACAATGAGGAGGTTCTCTTTTCCATTGACGGTCTGGAAAAACCAAATGAAGATGGTGTTTCTATTCTCTTCTACTTGCAAAAGATTTTCCCAGATGAATGGAACAACTTTCTTCAGCGTGTGAATCGCAGCAGCGAAGAGGAACTAAAAGTGTCTGATGAATTGGAAGAAGATCTTCGCCTATGGGCATCATATAGAGGCCAAACTTTGACTCGTACTGTACGAGGGATGATGTACTACCGGAAAGCTTTGGAGCTTCAGGCTTTTCTGGATATGGCCAAAGAGGAGGATTTAATGGAAGGCTATAAGGCTATAGAGTTGAATTCAGAAGATCAATCAAAGGAGGGAAGATCACTATGGGCACAATGTCAAGCAGTAGCTGATATGAAATTCACATATGTGGTTTCATGCCAAAAGTATGGTATTCAGAAGCGATCTGGTGATCTTCGTGCACAGGACATTCTGAGGCTCATGACAACATACCCATCATTACGAGTGGCATATATTGATGAGGTTGAAGAACCTAGCAAAGATAGGTCTCAGAAGATAAACCAAAAGGCTTATTATTCTACTTTAGTAAAGGCTGCTATGCCAAAGTCAATTGACTCTTCAGAGCCAGTACAAAACCTTGACCAGGTTATTTATCGCATAAAGCTTCCAGGACCTGCTATTTTGGGAGAGGGAAAGCCAGAAAATCAAAATCATGCCATTATTTTCACGCGTGGAGAAGGCTTGCAGACAATTGACATGAACCAGGATAATTACATGGAAGAAGCTTTGAAAATGAGGAATTTGCTACAAGAATTTCTCAAGCATGATGGCGTGAGGCACCCTACAATTCTTGGACTTAGGGAGCATATATTTACAGGAAGTGTTTCTTCTCTAGCTTGGTTCATGTCAAATCAGGAGAACAGTTTTGTGACTATTGGTCAAAGACTATTGGCCAACCCACTCAGGGTTCGATTTCATTATGGGCATCCCGATGTGTTTGATAGGCTCTTTCACCTTAGTAGAGGGGGTGTCAGTAAGGCATCCAAGGTTATCAATTTGAGTGAAGACATTTTTGCTGGCTTTAATTCCACTCTCCGTGAAGGCAATGTAACTCATCACGAATACATACAAGTGGGGAAGGGGAGAGATGTCGGTCTTAACCAAATCTCCATGTTTGAGGCAAAAATTGCTAATGGCAACGGGGAGCAGACACTCAGTCGAGATATATACCGACTTGGACATCGTTTTGACTTTTTCCGTATGCTGTCATGTTATTTCACCACAATTGGTTTCTACTTCAGTACTTTGATCACTGTGCTTACAGTTTATGTCTTCCTTTATGGTCGCCTATACCTGGTTCTTAGTGGACTTGAAGAAGGTTTGAGCACTCAAGCAGCAATTCGAGATAATAAGCCTCTTCAAGTTGCTCTTGCTTCTCAATCATTTGTTCAAATAGGGTTTTTGATGGCTTTGCCTATGTTGATGGAAATTGGCTTGGAAAAGGGTTTCCGAACTGCATTAAGTGAATTCGTGTTGATGCAATTGCAACTGGCCCCAGTATTTTTCACATTCTCACTTGGGACAAAGACCCACTATTATGGACGGACATTACTGCATGGTGGTGCCAAATATAGATCTACAGGTCGTGGGTTTGTGGTGTTCCATGCCAAGTTTGCCGACAACTATAGGCTCTACTCCCGCAGCCACTTTGTTAAGGGTATTGAGCTCTTGATTTTACTTGTCGTGTACCAGATCTTTGGTCATACTTATAGAAGTGCTGTTGCCTACATTTTGATCACTGTATCTATGTGGTTTATGGTGGTTACCTGGCTTTTTGCTCCCTTCCTGTTCAATCCATCTGGTTTTGAGTGGCAAAAGATTGTTGATGATTGGACTGATTGGAATAAGTGGATAAGCAACCGTGGAGGTATAGGTGTTCCACCTGAAAAAAGTTGGGAATCATGGTGGGAGGAGGAACAAGAACATCTTCGAGATTCTGGAAAACGTGGTATTGTAGTTGAGATACTGCTATCTGTACGATTCTTTATCTATCAATATGGGCTCGTATATCACTTAAACATTGCAAAGAAAACCAAGAGTGTCCTGGTCTATGGTATCTCCTGGCTGGTGATCGTCCTTATTTTGTTTGTCATGAAGACTGTATCTGTTGGTAGGAGAAAGTTCAGTGCTGAGTACCAACTTGTATTCCGGCTGATCAAGGGATTAATATTCATTACTTTTGTGTCCATTTTGGTCACTTTGATTGTGCTGCCTCACATGACCCTGCAGGACATCATTGTTTGCATTCTTGCTTTCATGCCAACTGGTTGGGGGATGCTTATGATTGCCCAAGCTTGCAAAGGCTTGGTTCAAAAAGCTGGTTTATGGCCATCAGTTCGGACTCTTGCCCGTGGCTTTGAGATAGTTATGGGCTTGCTTCTGTTCACCCCAGTTGCATTCTTGGCCTGGTTTCCTTTTGTTTCAGAATTCCAAACTCGTATGCTCTTCAACCAAGCATTCAGTAGAGGTTTACAGATTTCTCGTATTCTTGGCGGGCAACGCAAGGATCGTTCATCCCGGAACAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FKS1_dom1 Glucan_synthase Vta1

Protein Analysis

1851

Amino Acids

213.85

Weight (kDa)

9.2

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKS1_dom1 PF14288 217 - 329 3.2e-43 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 752 - 944 1.4e-65 Callose synthase 1 domain
Glucan_synthase PF02364 951 - 1041 8.7e-28 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1043 - 1744 1.4e-211 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1265, 2183, 2444
Acc16I TGCGCA 1 cut(s) 2136
Acc36I ACCTGC 1 cut(s) 3643
AccB1I GGYRCC 2 cut(s) 3798, 4583
AccB7I CCANNNNNTGG 1 cut(s) 815
AccI GTMKAC 2 cut(s) 1421, 1518
AccII CGCG 2 cut(s) 30, 3691
AciI CCGC 7 cut(s) 89, 1144, 1330, 2036, 2083, 4660, 5514
AclWI GGATC 6 cut(s) 266, 2184, 2272, 2488, 2595, 5535
AcoI YGGCCR 6 cut(s) 90, 983, 2298, 3246, 3912, 5348
AcuI CTGAAG 5 cut(s) 2087, 3068, 3212, 3564, 4243
AfiI CCNNNNNNNGG 9 cut(s) 791, 815, 924, 1757, 1899, 3604, 3925, 4315, 5545
AflIII ACRYGT 2 cut(s) 2776, 3689
AgeI ACCGGT 1 cut(s) 1405
AhdI GACNNNNNGTC 2 cut(s) 3897, 4176
AjiI CACGTC 1 cut(s) 2851
AleI CACNNNNGTG 1 cut(s) 3368
AloI GAACNNNNNNTCC 2 cut(s) 1281, 1313
Alw21I GWGCWC 8 cut(s) 112, 459, 486, 1667, 2518, 3424, 4349, 4689
Alw26I GTCTC 2 cut(s) 1640, 3513
Alw44I GTGCAC 3 cut(s) 108, 482, 3420
AlwI GGATC 6 cut(s) 266, 2184, 2272, 2488, 2595, 5535
AlwNI CAGNNNCTG 4 cut(s) 1001, 1385, 3350, 3635
Ama87I CYCGRG 2 cut(s) 1204, 2689
ApaLI GTGCAC 3 cut(s) 108, 482, 3420
AseI ATTAAT 1 cut(s) 5186
AsiGI ACCGGT 1 cut(s) 1405
Asp700I GAANNNNTTC 4 cut(s) 560, 3144, 3844, 4947
AspLEI GCGC 4 cut(s) 648, 1489, 2050, 2137
AspS9I GGNCC 9 cut(s) 595, 1207, 1760, 2200, 2425, 2650, 2692, 3632, 4513
AsuC2I CCSGG 1 cut(s) 5539
AsuHPI GGTGA 8 cut(s) 833, 1518, 2498, 3422, 3965, 4231, 5101, 5398
AsuNHI GCTAGC 1 cut(s) 2270
AvaI CYCGRG 2 cut(s) 1204, 2689
AvaII GGWCC 5 cut(s) 1760, 2200, 2425, 2650, 3632
AxyI CCTNAGG 1 cut(s) 363
BaeGI GKGCMC 5 cut(s) 112, 486, 538, 3334, 3424
BalI TGGCCA 4 cut(s) 2300, 3248, 3914, 5350
BanI GGYRCC 2 cut(s) 3798, 4583
BanII GRGCYC 3 cut(s) 1864, 4689, 5025
BbsI GAAGAC 4 cut(s) 344, 4032, 4285, 5123
Bbv12I GWGCWC 8 cut(s) 112, 459, 486, 1667, 2518, 3424, 4349, 4689
BciVI GTATCC 1 cut(s) 1882
BclI TGATCA 5 cut(s) 508, 2716, 4269, 4755, 5175
BcnI CCSGG 1 cut(s) 5539
BcoDI GTCTC 2 cut(s) 1640, 3513
BfmI CTRYAG 6 cut(s) 407, 1594, 3282, 4599, 4645, 5246
BfuAI ACCTGC 1 cut(s) 3643
BfuI GTATCC 1 cut(s) 1882
BglII AGATCT 4 cut(s) 1985, 3142, 4595, 4713
BmcAI AGTACT 2 cut(s) 950, 4264
Bme18I GGWCC 5 cut(s) 1760, 2200, 2425, 2650, 3632
BmeRI GACNNNNNGTC 2 cut(s) 3897, 4176
BmeT110I CYCGRG 2 cut(s) 1204, 2689
BmgBI CACGTC 1 cut(s) 2851
BmgT120I GGNCC 9 cut(s) 595, 1207, 1760, 2200, 2425, 2650, 2692, 3632, 4513
BmiI GGNNCC 7 cut(s) 249, 1761, 1863, 2426, 3800, 4515, 4585
BmrI ACTGGG 3 cut(s) 913, 4511, 5405
BmtI GCTAGC 1 cut(s) 2274
BmuI ACTGGG 3 cut(s) 913, 4511, 5405
BpiI GAAGAC 4 cut(s) 344, 4032, 4285, 5123
BpmI CTGGAG 3 cut(s) 2639, 2772, 2904
Bpu10I CCTNAGC 1 cut(s) 1368
BpuEI CTTGAG 3 cut(s) 1601, 3764, 4335
BpuMI CCSGG 1 cut(s) 5539
Bsa29I ATCGAT 1 cut(s) 1078
BsaAI YACGTR 1 cut(s) 1787
BsaBI GATNNNNATC 1 cut(s) 2721
BsaI GGTCTC 1 cut(s) 3513
BsaWI WCCGGW 3 cut(s) 972, 1405, 3210
BsaXI ACNNNNNCTCC 4 cut(s) 786, 816, 4112, 4142
Bsc4I CCNNNNNNNGG 9 cut(s) 791, 815, 924, 1757, 1899, 3604, 3925, 4315, 5545
Bse118I RCCGGY 1 cut(s) 1405
Bse1I ACTGG 7 cut(s) 919, 1801, 3587, 4515, 4517, 5290, 5411
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 4 cut(s) 2000, 2317, 4072, 5040
Bse8I GATNNNNATC 1 cut(s) 2721
BseCI ATCGAT 1 cut(s) 1078
BseGI GGATG 9 cut(s) 397, 532, 2709, 2855, 3204, 3946, 4001, 5301, 5534
BseJI GATNNNNATC 1 cut(s) 2721
BseLI CCNNNNNNNGG 9 cut(s) 791, 815, 924, 1757, 1899, 3604, 3925, 4315, 5545
BseMI GCAATG 4 cut(s) 2000, 2317, 4072, 5040
BseMII CTCAG 7 cut(s) 1463, 2451, 3425, 3524, 3937, 4189, 5142
BseNI ACTGG 7 cut(s) 919, 1801, 3587, 4515, 4517, 5290, 5411
BseRI GAGGAG 4 cut(s) 2186, 2993, 3269, 4946
BseSI GKGCMC 5 cut(s) 112, 486, 538, 3334, 3424
BseX3I CGGCCG 1 cut(s) 90
BseYI CCCAGC 2 cut(s) 770, 1541
BsgI GTGCAG 2 cut(s) 1014, 1092
Bsh1236I CGCG 2 cut(s) 30, 3691
Bsh1285I CGRYCG 1 cut(s) 93
BshNI GGYRCC 2 cut(s) 3798, 4583
BshTI ACCGGT 1 cut(s) 1405
BshVI ATCGAT 1 cut(s) 1078
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 8 cut(s) 112, 459, 486, 1667, 2518, 3424, 4349, 4689
BsiHKCI CYCGRG 2 cut(s) 1204, 2689
BsiSI CCGG 6 cut(s) 598, 973, 1406, 3211, 5170, 5539
BslFI GGGAC 3 cut(s) 2065, 2438, 4555
BslI CCNNNNNNNGG 9 cut(s) 791, 815, 924, 1757, 1899, 3604, 3925, 4315, 5545
BsmAI GTCTC 2 cut(s) 1640, 3513
BsmBI CGTCTC 1 cut(s) 1640
BsmFI GGGAC 3 cut(s) 2065, 2438, 4555
Bso31I GGTCTC 1 cut(s) 3513
BsoBI CYCGRG 2 cut(s) 1204, 2689
Bsp19I CCATGG 1 cut(s) 2844
BspACI CCGC 7 cut(s) 89, 1144, 1330, 2036, 2083, 4660, 5514
BspCNI CTCAG 7 cut(s) 1462, 2452, 3426, 3523, 3936, 4188, 5143
BspDI ATCGAT 1 cut(s) 1078
BspFNI CGCG 2 cut(s) 30, 3691
BspHI TCATGA 4 cut(s) 214, 1776, 3441, 5112
BspLI GGNNCC 7 cut(s) 249, 1761, 1863, 2426, 3800, 4515, 4585
BspMAI CTGCAG 2 cut(s) 411, 5250
BspMI ACCTGC 1 cut(s) 3643
BspOI GCTAGC 1 cut(s) 2274
BspPI GGATC 6 cut(s) 266, 2184, 2272, 2488, 2595, 5535
BspQI GCTCTTC 3 cut(s) 339, 2523, 5471
BspT107I GGYRCC 2 cut(s) 3798, 4583
BspTNI GGTCTC 1 cut(s) 3513
BsrDI GCAATG 4 cut(s) 2000, 2317, 4072, 5040
BsrFI RCCGGY 1 cut(s) 1405
BsrI ACTGG 7 cut(s) 919, 1801, 3587, 4515, 4517, 5290, 5411
BssAI RCCGGY 1 cut(s) 1405
BssNAI GTATAC 1 cut(s) 1422
BssT1I CCWWGG 6 cut(s) 120, 356, 1954, 2301, 2844, 4005
Bst1107I GTATAC 1 cut(s) 1422
Bst6I CTCTTC 8 cut(s) 339, 822, 2523, 3044, 3102, 3583, 4383, 5471
BstAPI GCANNNNNTGC 3 cut(s) 637, 2132, 4148
BstBAI YACGTR 1 cut(s) 1787
BstDSI CCRYGG 4 cut(s) 2680, 2844, 4882, 5371
BstEII GGTNACC 3 cut(s) 615, 2486, 4783
BstENI CCTNNNNNAGG 1 cut(s) 3602
BstF5I GGATG 9 cut(s) 397, 532, 2709, 2855, 3204, 3946, 4001, 5301, 5534
BstFNI CGCG 2 cut(s) 30, 3691
BstHHI GCGC 4 cut(s) 648, 1489, 2050, 2137
BstMAI GTCTC 2 cut(s) 1640, 3513
BstMCI CGRYCG 1 cut(s) 93
BstNSI RCATGY 4 cut(s) 1150, 2362, 2617, 2780
BstPI GGTNACC 3 cut(s) 615, 2486, 4783
BstSFI CTRYAG 6 cut(s) 407, 1594, 3282, 4599, 4645, 5246
BstSLI GKGCMC 5 cut(s) 112, 486, 538, 3334, 3424
BstSNI TACGTA 1 cut(s) 1787
BstUI CGCG 2 cut(s) 30, 3691
BstV2I GAAGAC 4 cut(s) 344, 4032, 4285, 5123
BstX2I RGATCY 6 cut(s) 1985, 2176, 2277, 3142, 4595, 4713
BstXI CCANNNNNNTGG 5 cut(s) 3069, 3389, 4559, 4718, 5289
BstYI RGATCY 6 cut(s) 1985, 2176, 2277, 3142, 4595, 4713
BstZ17I GTATAC 1 cut(s) 1422
BstZI CGGCCG 1 cut(s) 90
Bsu15I ATCGAT 1 cut(s) 1078
Bsu36I CCTNAGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1882
BsuTUI ATCGAT 1 cut(s) 1078
BtgI CCRYGG 4 cut(s) 2680, 2844, 4882, 5371
BtrI CACGTC 1 cut(s) 2851
BtsCI GGATG 9 cut(s) 397, 532, 2709, 2855, 3204, 3946, 4001, 5301, 5534
BtsIMutI CAGTG 7 cut(s) 926, 966, 1808, 2255, 4272, 4758, 5152
BveI ACCTGC 1 cut(s) 3643
CaiI CAGNNNCTG 4 cut(s) 1001, 1385, 3350, 3635
CciI TCATGA 4 cut(s) 214, 1776, 3441, 5112
CfoI GCGC 4 cut(s) 648, 1489, 2050, 2137
Cfr10I RCCGGY 1 cut(s) 1405
Cfr13I GGNCC 9 cut(s) 595, 1207, 1760, 2200, 2425, 2650, 2692, 3632, 4513
ClaI ATCGAT 1 cut(s) 1078
CsiI ACCWGGT 2 cut(s) 3603, 4314
CspAI ACCGGT 1 cut(s) 1405
DraI TTTAAA 1 cut(s) 159
DriI GACNNNNNGTC 2 cut(s) 3897, 4176
EaeI YGGCCR 6 cut(s) 90, 983, 2298, 3246, 3912, 5348
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 8 cut(s) 339, 822, 2523, 3044, 3102, 3583, 4383, 5471
Eam1105I GACNNNNNGTC 2 cut(s) 3897, 4176
EarI CTCTTC 8 cut(s) 339, 822, 2523, 3044, 3102, 3583, 4383, 5471
EciI GGCGGA 1 cut(s) 1345
Ecl136II GAGCTC 1 cut(s) 4687
EclXI CGGCCG 1 cut(s) 90
Eco105I TACGTA 1 cut(s) 1787
Eco130I CCWWGG 6 cut(s) 120, 356, 1954, 2301, 2844, 4005
Eco24I GRGCYC 3 cut(s) 1864, 4689, 5025
Eco31I GGTCTC 1 cut(s) 3513
Eco47I GGWCC 5 cut(s) 1760, 2200, 2425, 2650, 3632
Eco52I CGGCCG 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 4687
Eco57I CTGAAG 5 cut(s) 2087, 3068, 3212, 3564, 4243
Eco81I CCTNAGG 1 cut(s) 363
Eco88I CYCGRG 2 cut(s) 1204, 2689
Eco91I GGTNACC 3 cut(s) 615, 2486, 4783
EcoICRI GAGCTC 1 cut(s) 4687
EcoNI CCTNNNNNAGG 1 cut(s) 3602
EcoO109I RGGNCCY 1 cut(s) 3632
EcoO65I GGTNACC 3 cut(s) 615, 2486, 4783
EcoRI GAATTC 5 cut(s) 2501, 2896, 3292, 4487, 5447
EcoT14I CCWWGG 6 cut(s) 120, 356, 1954, 2301, 2844, 4005
EcoT22I ATGCAT 2 cut(s) 1659, 2364
EcoT38I GRGCYC 3 cut(s) 1864, 4689, 5025
ErhI CCWWGG 6 cut(s) 120, 356, 1954, 2301, 2844, 4005
Esp3I CGTCTC 1 cut(s) 1640
FalI AAGNNNNNCTT 8 cut(s) 3129, 3161, 3832, 3864, 4374, 4406, 4932, 4964
FaqI GGGAC 3 cut(s) 2065, 2438, 4555
FauI CCCGC 3 cut(s) 2090, 4667, 5507
FauNDI CATATG 2 cut(s) 741, 3367
FbaI TGATCA 5 cut(s) 508, 2716, 4269, 4755, 5175
FblI GTMKAC 2 cut(s) 1421, 1518
FokI GGATG 9 cut(s) 404, 539, 2716, 2842, 3211, 3933, 3988, 5308, 5521
FriOI GRGCYC 3 cut(s) 1864, 4689, 5025
FspI TGCGCA 1 cut(s) 2136
GlaI GCGC 4 cut(s) 647, 1488, 2049, 2136
GsaI CCCAGC 2 cut(s) 774, 1545
GsuI CTGGAG 3 cut(s) 2639, 2772, 2904
HapII CCGG 6 cut(s) 598, 973, 1406, 3211, 5170, 5539
HhaI GCGC 4 cut(s) 648, 1489, 2050, 2137
Hin6I GCGC 4 cut(s) 646, 1487, 2048, 2135
HinP1I GCGC 4 cut(s) 646, 1487, 2048, 2135
HincII GTYRAC 2 cut(s) 1096, 2467
HindII GTYRAC 2 cut(s) 1096, 2467
HindIII AAGCTT 9 cut(s) 75, 557, 1135, 1248, 1283, 3216, 3623, 3744, 5313
HpaI GTTAAC 1 cut(s) 1096
HpaII CCGG 6 cut(s) 598, 973, 1406, 3211, 5170, 5539
HphI GGTGA 8 cut(s) 833, 1518, 2498, 3422, 3965, 4231, 5101, 5398
Hpy99I CGWCG 2 cut(s) 1035, 1953
HpyCH4IV ACGT 5 cut(s) 867, 1030, 1786, 2850, 4966
HpySE526I ACGT 5 cut(s) 867, 1030, 1786, 2850, 4966
HspAI GCGC 4 cut(s) 646, 1487, 2048, 2135
Ksp22I TGATCA 5 cut(s) 508, 2716, 4269, 4755, 5175
KspAI GTTAAC 1 cut(s) 1096
LguI GCTCTTC 3 cut(s) 339, 2523, 5471
LmnI GCTCC 8 cut(s) 454, 891, 1867, 2753, 3223, 3823, 4165, 4804
MabI ACCWGGT 2 cut(s) 3603, 4314
MaeII ACGT 5 cut(s) 867, 1030, 1786, 2850, 4966
MfeI CAATTG 4 cut(s) 3570, 3711, 4245, 4502
MflI RGATCY 6 cut(s) 1985, 2176, 2277, 3142, 4595, 4713
MlsI TGGCCA 4 cut(s) 2300, 3248, 3914, 5350
MluI ACGCGT 1 cut(s) 3689
MluNI TGGCCA 4 cut(s) 2300, 3248, 3914, 5350
MlyI GAGTC 6 cut(s) 790, 847, 2846, 3175, 3569, 5356
MmeI TCCRAC 3 cut(s) 504, 1787, 2883
Mox20I TGGCCA 4 cut(s) 2300, 3248, 3914, 5350
Mph1103I ATGCAT 2 cut(s) 1659, 2364
MroXI GAANNNNTTC 4 cut(s) 560, 3144, 3844, 4947
MscI TGGCCA 4 cut(s) 2300, 3248, 3914, 5350
MslI CAYNNNNRTG 6 cut(s) 2849, 3368, 3951, 4091, 4557, 4581
Msp20I TGGCCA 4 cut(s) 2300, 3248, 3914, 5350
MspA1I CMGCKG 2 cut(s) 1382, 2036
MspI CCGG 6 cut(s) 598, 973, 1406, 3211, 5170, 5539
MunI CAATTG 4 cut(s) 3570, 3711, 4245, 4502
MvnI CGCG 2 cut(s) 30, 3691
NciI CCSGG 1 cut(s) 5539
NcoI CCATGG 1 cut(s) 2844
NdeI CATATG 2 cut(s) 741, 3367
NheI GCTAGC 1 cut(s) 2270
NlaIV GGNNCC 7 cut(s) 249, 1761, 1863, 2426, 3800, 4515, 4585
NmuCI GTSAC 8 cut(s) 850, 1524, 1623, 1711, 2486, 2626, 3889, 5215
NsbI TGCGCA 1 cut(s) 2136
NsiI ATGCAT 2 cut(s) 1659, 2364
NspI RCATGY 4 cut(s) 1150, 2362, 2617, 2780
OliI CACNNNNGTG 1 cut(s) 3368
PagI TCATGA 4 cut(s) 214, 1776, 3441, 5112
PciI ACATGT 1 cut(s) 2776
PciSI GCTCTTC 3 cut(s) 339, 2523, 5471
PcsI WCGNNNNNNNCGW 2 cut(s) 3190, 5527
PdmI GAANNNNTTC 4 cut(s) 560, 3144, 3844, 4947
PflMI CCANNNNNTGG 1 cut(s) 815
PfoI TCCNGGA 3 cut(s) 2607, 3628, 5537
PinAI ACCGGT 1 cut(s) 1405
PleI GAGTC 6 cut(s) 789, 847, 2845, 3175, 3569, 5356
PpsI GAGTC 6 cut(s) 789, 847, 2845, 3175, 3569, 5356
Ppu21I YACGTR 1 cut(s) 1787
PpuMI RGGWCCY 1 cut(s) 3632
PscI ACATGT 1 cut(s) 2776
PshBI ATTAAT 1 cut(s) 5186
PsiI TTATAA 3 cut(s) 1265, 2183, 2444
Psp124BI GAGCTC 1 cut(s) 4689
Psp5II RGGWCCY 1 cut(s) 3632
PspEI GGTNACC 3 cut(s) 615, 2486, 4783
PspFI CCCAGC 2 cut(s) 770, 1541
PspN4I GGNNCC 7 cut(s) 249, 1761, 1863, 2426, 3800, 4515, 4585
PspPI GGNCC 9 cut(s) 595, 1207, 1760, 2200, 2425, 2650, 2692, 3632, 4513
PspPPI RGGWCCY 1 cut(s) 3632
PstI CTGCAG 2 cut(s) 411, 5250
PstNI CAGNNNCTG 4 cut(s) 1001, 1385, 3350, 3635
PsuI RGATCY 6 cut(s) 1985, 2176, 2277, 3142, 4595, 4713
PvuII CAGCTG 1 cut(s) 1382
RseI CAYNNNNRTG 6 cut(s) 2849, 3368, 3951, 4091, 4557, 4581
SacI GAGCTC 1 cut(s) 4689
SapI GCTCTTC 3 cut(s) 339, 2523, 5471
Sau96I GGNCC 9 cut(s) 595, 1207, 1760, 2200, 2425, 2650, 2692, 3632, 4513
SbfI CCTGCAGG 1 cut(s) 5250
ScaI AGTACT 2 cut(s) 950, 4264
SchI GAGTC 6 cut(s) 790, 847, 2846, 3175, 3569, 5356
SdaI CCTGCAGG 1 cut(s) 5250
SexAI ACCWGGT 2 cut(s) 3603, 4314
SfcI CTRYAG 6 cut(s) 407, 1594, 3282, 4599, 4645, 5246
SinI GGWCC 5 cut(s) 1760, 2200, 2425, 2650, 3632
SmiMI CAYNNNNRTG 6 cut(s) 2849, 3368, 3951, 4091, 4557, 4581
SmlI CTYRAG 3 cut(s) 1580, 3779, 4350
SmoI CTYRAG 3 cut(s) 1580, 3779, 4350
SnaBI TACGTA 1 cut(s) 1787
Sse8387I CCTGCAGG 1 cut(s) 5250
SsiI CCGC 7 cut(s) 89, 1144, 1330, 2036, 2083, 4660, 5514
SspI AATATT 4 cut(s) 1224, 1278, 1923, 5190
SstI GAGCTC 1 cut(s) 4689
StyI CCWWGG 6 cut(s) 120, 356, 1954, 2301, 2844, 4005
TaiI ACGT 5 cut(s) 870, 1033, 1789, 2853, 4969
TaqI TCGA 7 cut(s) 1033, 1078, 2894, 3931, 4180, 4363, 4951
TaqII GACCGA 1 cut(s) 4103
TatI WGTACW 5 cut(s) 948, 1683, 3204, 3589, 4262
TauI GCSGC 1 cut(s) 92
TscAI CASTG 7 cut(s) 926, 966, 1808, 2255, 4279, 4765, 5152
TseFI GTSAC 8 cut(s) 850, 1524, 1623, 1711, 2486, 2626, 3889, 5215
Tsp45I GTSAC 8 cut(s) 850, 1524, 1623, 1711, 2486, 2626, 3889, 5215
TspGWI ACGGA 7 cut(s) 1937, 1981, 2486, 2697, 4046, 4208, 4579
TspRI CASTG 7 cut(s) 926, 966, 1808, 2255, 4279, 4765, 5152
Van91I CCANNNNNTGG 1 cut(s) 815
VneI GTGCAC 3 cut(s) 108, 482, 3420
VpaK11BI GGWCC 5 cut(s) 1760, 2200, 2425, 2650, 3632
VspI ATTAAT 1 cut(s) 5186
XagI CCTNNNNNAGG 1 cut(s) 3602
XbaI TCTAGA 1 cut(s) 1300
XceI RCATGY 4 cut(s) 1150, 2362, 2617, 2780
XcmI CCANNNNNNNNNTGG 2 cut(s) 3001, 5062
XmiI GTMKAC 2 cut(s) 1421, 1518
XmnI GAANNNNTTC 4 cut(s) 560, 3144, 3844, 4947
ZrmI AGTACT 2 cut(s) 950, 4264
Zsp2I ATGCAT 2 cut(s) 1659, 2364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.