AT5G13020

emsy N terminus domain-containing protein ENT domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007058
Physical Location & Seq
Reverse (-)
4129154 .. 4133023
3870 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13020.2

Sequence Viewer

Length: 1194 bp
ATGGATTACCGACCTTCTGATAGTAGTGGTACAGATGATGACCTGCCTCCATCTCATCAAGGTAGATATCAAAGAAACGCCAGACCTACCGGGAATGGAAGGCCTTCAGTTCTCAATTCTGCTCCTTTATCACGGGTGCACAATGAAATGGAAACTCAAATTCATCTCATTGAGCAAGAAGCTTATAGCTCGATACTCCGCGCATTTAAAGCCCAGTCCGATGCTATTACCTGGGAGAAAGAAAGTTTGATCACTGAACTCAGAAAAGAACTTCGAGTGTCTGATGAGGAACACAGAGAGCTGTTATCAAGGGTTAACGCTGATGAAATGATCAGGCGAATAAGGGAATGGAGAAAGGCAAACAGCCTTCAGTCTAGTGTTCCTCAGCTGGTTCATGATGCTCCGAGTCCGGCTGTATCAGGATCACGTAAGAAGCAAAAGACATCACAATCAATCGCCTCATTAGCGATGGGCCCACCATCTCCTTCTTTGCACCCTTCAATGCAACCATCGTCATCTGCACTAAGAAGGGGAGGTCCTCCACCAGGTCCAAAGACCAAGAAGCCAAAGACATCGATGCAGTACCCATCTACAGGCATTGCTGGAAGGCCCCAGGCTGGCGCTTTAACAAATGAACCAGGTGAATCGGGATCATATGACCCGTTGGTTGGAAGGAAGGTATGGACGAAGTGGCCTGATGACAACCAATACTACGAAGCTGTTATAACTGACTACAACCCTGTTGAGGGGCGTCATGCTTTAGTGTATGATATTAACTCTGCGAATGAAACCTGGGAATGGGTAAATCTTAAAGAGATATCTCCGGGAGATATCAGATGGGAAGGTGAAGATCCTGGGATTTCTCGTAAAGGAGGACATCCTGGGCAAGGCCGTGGAACAAAAACCATGGCTCGTGGTGGTCCTGCAAGCAATGCGGGTGGTAGAGGTAGGGGAAGCATGAGGATGCAGCAACCTAAGACACAGAATGGCATCGGAAAGAAAGCTTTAGGCGAAATCGAAATTCTCCACACTGAGACATTGTTAAAAGAGGTGGAAAAAGTTTTTGGGTCAGTTAACCCCAACCCAGCAGAGGTAGAGAAGGCAAAGAGAGTGCTGAGAGATCATGAACTAGCTCTTATGGATGCCATCGCAAAGCTTGAAGAAATATCAGATGGAGAAAGCGGTAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

397

Amino Acids

43.65

Weight (kDa)

8.52

Isoelectric Point (pI)

49.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 52 - 120 5.6e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 725
Acc36I ACCTGC 1 cut(s) 51
AccII CGCG 1 cut(s) 201
AciI CCGC 3 cut(s) 199, 935, 1182
AclWI GGATC 3 cut(s) 430, 658, 845
AcsI RAATTY 2 cut(s) 159, 1020
AcuI CTGAAG 2 cut(s) 90, 353
AcyI GRCGYC 1 cut(s) 751
AfaI GTAC 2 cut(s) 31, 584
AfiI CCNNNNNNNGG 9 cut(s) 545, 593, 617, 668, 745, 746, 798, 887, 1090
AgsI TTSAA 2 cut(s) 501, 1160
AjnI CCWGG 7 cut(s) 230, 544, 612, 637, 791, 853, 880
AjuI GAANNNNNNNTTGG 2 cut(s) 1047, 1079
AluBI AGCT 8 cut(s) 182, 189, 301, 388, 719, 1004, 1133, 1156
AluI AGCT 8 cut(s) 182, 189, 301, 388, 719, 1004, 1133, 1156
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 1 cut(s) 1028
Alw44I GTGCAC 1 cut(s) 137
AlwI GGATC 3 cut(s) 430, 658, 845
AoxI GGCC 5 cut(s) 101, 472, 608, 692, 889
ApaI GGGCCC 1 cut(s) 476
ApaLI GTGCAC 1 cut(s) 137
ApeKI GCWGC 1 cut(s) 967
ApoI RAATTY 2 cut(s) 159, 1020
Asp700I GAANNNNTTC 1 cut(s) 103
AspLEI GCGC 2 cut(s) 203, 623
AspS9I GGNCC 6 cut(s) 472, 473, 536, 548, 609, 920
AsuC2I CCSGG 2 cut(s) 91, 825
AsuHPI GGTGA 2 cut(s) 653, 857
AvaII GGWCC 3 cut(s) 536, 548, 920
BaeGI GKGCMC 2 cut(s) 141, 476
BanII GRGCYC 1 cut(s) 476
BauI CACGAG 1 cut(s) 912
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 1 cut(s) 979
BccI CCATC 8 cut(s) 58, 463, 487, 517, 595, 831, 1154, 1166
BceAI ACGGC 1 cut(s) 876
BciT130I CCWGG 7 cut(s) 232, 546, 614, 639, 793, 855, 882
BclI TGATCA 2 cut(s) 249, 330
BcnI CCSGG 2 cut(s) 91, 825
BcoDI GTCTC 1 cut(s) 1028
BfaI CTAG 2 cut(s) 375, 1130
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 51
BisI GCNGC 1 cut(s) 968
BlsI GCNGC 1 cut(s) 969
Bme1390I CCNGG 9 cut(s) 91, 232, 546, 614, 639, 793, 825, 855, 882
Bme18I GGWCC 3 cut(s) 536, 548, 920
BmgT120I GGNCC 6 cut(s) 472, 473, 536, 548, 609, 920
BmiI GGNNCC 2 cut(s) 474, 611
BmrFI CCNGG 9 cut(s) 91, 232, 546, 614, 639, 793, 825, 855, 882
BmrI ACTGGG 1 cut(s) 208
BmsI GCATC 6 cut(s) 211, 388, 567, 954, 999, 1132
BmuI ACTGGG 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 91, 825
Bsa29I ATCGAT 1 cut(s) 575
BsaAI YACGTR 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 751
BsaJI CCNNGG 7 cut(s) 231, 612, 792, 854, 881, 892, 906
Bsc4I CCNNNNNNNGG 9 cut(s) 545, 593, 617, 668, 745, 746, 798, 887, 1090
Bse1I ACTGG 1 cut(s) 214
Bse3DI GCAATG 2 cut(s) 597, 937
BseBI CCWGG 7 cut(s) 232, 546, 614, 639, 793, 855, 882
BseCI ATCGAT 1 cut(s) 575
BseDI CCNNGG 7 cut(s) 231, 612, 792, 854, 881, 892, 906
BseGI GGATG 3 cut(s) 877, 969, 1147
BseLI CCNNNNNNNGG 9 cut(s) 545, 593, 617, 668, 745, 746, 798, 887, 1090
BseMI GCAATG 2 cut(s) 597, 937
BseMII CTCAG 4 cut(s) 274, 398, 1023, 1106
BseNI ACTGG 1 cut(s) 214
BseSI GKGCMC 2 cut(s) 141, 476
BseXI GCAGC 1 cut(s) 979
BseYI CCCAGC 1 cut(s) 1084
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 201
BshFI GGCC 5 cut(s) 103, 474, 610, 694, 891
BshVI ATCGAT 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 3 cut(s) 90, 410, 824
BslI CCNNNNNNNGG 9 cut(s) 545, 593, 617, 668, 745, 746, 798, 887, 1090
BsmAI GTCTC 1 cut(s) 1028
BsnI GGCC 5 cut(s) 103, 474, 610, 694, 891
Bsp120I GGGCCC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 141, 476
Bsp143I GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
Bsp19I CCATGG 1 cut(s) 906
BspACI CCGC 3 cut(s) 199, 935, 1182
BspANI GGCC 5 cut(s) 103, 474, 610, 694, 891
BspCNI CTCAG 4 cut(s) 273, 397, 1024, 1107
BspDI ATCGAT 1 cut(s) 575
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 2 cut(s) 394, 1123
BspLI GGNNCC 2 cut(s) 474, 611
BspMI ACCTGC 1 cut(s) 51
BspPI GGATC 3 cut(s) 430, 658, 845
BsrDI GCAATG 2 cut(s) 597, 937
BsrI ACTGG 1 cut(s) 214
BssECI CCNNGG 7 cut(s) 231, 612, 792, 854, 881, 892, 906
BssMI GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
BssNI GRCGYC 1 cut(s) 751
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 1 cut(s) 906
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 7 cut(s) 232, 546, 614, 639, 793, 855, 882
BstACI GRCGYC 1 cut(s) 751
BstAPI GCANNNNNTGC 1 cut(s) 932
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 619, 928
BstDEI CTNAG 6 cut(s) 260, 384, 524, 975, 1032, 1115
BstDSI CCRYGG 2 cut(s) 892, 906
BstENI CCTNNNNNAGG 2 cut(s) 543, 885
BstF5I GGATG 3 cut(s) 877, 969, 1147
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 2 cut(s) 203, 623
BstKTI GATC 6 cut(s) 252, 333, 425, 653, 853, 1123
BstMAI GTCTC 1 cut(s) 1028
BstMBI GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 209, 464, 932
BstNI CCWGG 7 cut(s) 232, 546, 614, 639, 793, 855, 882
BstSCI CCNGG 9 cut(s) 89, 230, 544, 612, 637, 791, 823, 853, 880
BstSFI CTRYAG 1 cut(s) 591
BstSLI GKGCMC 2 cut(s) 141, 476
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 1 cut(s) 979
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
Bsu15I ATCGAT 1 cut(s) 575
BsuRI GGCC 5 cut(s) 103, 474, 610, 694, 891
BsuTUI ATCGAT 1 cut(s) 575
BtgI CCRYGG 2 cut(s) 892, 906
BtgZI GCGATG 2 cut(s) 482, 1132
BtsCI GGATG 3 cut(s) 877, 969, 1147
BtsIMutI CAGTG 2 cut(s) 252, 1029
BveI ACCTGC 1 cut(s) 51
Cac8I GCNNGC 2 cut(s) 619, 928
CciI TCATGA 2 cut(s) 394, 1123
CfoI GCGC 2 cut(s) 203, 623
Cfr13I GGNCC 6 cut(s) 472, 473, 536, 548, 609, 920
ClaI ATCGAT 1 cut(s) 575
CseI GACGC 1 cut(s) 740
CsiI ACCWGGT 2 cut(s) 544, 637
Csp6I GTAC 2 cut(s) 30, 583
CspCI CAANNNNNGTGG 2 cut(s) 919, 954
CviAII CATG 5 cut(s) 395, 755, 907, 958, 1124
CviQI GTAC 2 cut(s) 30, 583
DdeI CTNAG 6 cut(s) 260, 384, 524, 975, 1032, 1115
DpnI GATC 6 cut(s) 251, 332, 424, 652, 852, 1122
DpnII GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
DraI TTTAAA 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 906
Eco147I AGGCCT 1 cut(s) 103
Eco24I GRGCYC 1 cut(s) 476
Eco32I GATATC 3 cut(s) 68, 819, 832
Eco47I GGWCC 3 cut(s) 536, 548, 920
Eco57I CTGAAG 2 cut(s) 90, 353
EcoNI CCTNNNNNAGG 2 cut(s) 543, 885
EcoO109I RGGNCCY 2 cut(s) 536, 609
EcoRII CCWGG 7 cut(s) 230, 544, 612, 637, 791, 853, 880
EcoRV GATATC 3 cut(s) 68, 819, 832
EcoT14I CCWWGG 1 cut(s) 906
EcoT38I GRGCYC 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 906
FaeI CATG 5 cut(s) 398, 758, 910, 961, 1127
FatI CATG 5 cut(s) 394, 754, 906, 957, 1123
FauI CCCGC 1 cut(s) 928
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 2 cut(s) 249, 330
Fnu4HI GCNGC 1 cut(s) 968
FokI GGATG 3 cut(s) 864, 976, 1154
FriOI GRGCYC 1 cut(s) 476
Fsp4HI GCNGC 1 cut(s) 968
FspBI CTAG 2 cut(s) 375, 1130
GlaI GCGC 2 cut(s) 202, 622
GluI GCNGC 1 cut(s) 968
GsaI CCCAGC 1 cut(s) 1088
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 5 cut(s) 103, 474, 610, 694, 891
HapII CCGG 3 cut(s) 90, 410, 824
HgaI GACGC 1 cut(s) 740
HhaI GCGC 2 cut(s) 203, 623
Hin1I GRCGYC 1 cut(s) 751
Hin1II CATG 5 cut(s) 398, 758, 910, 961, 1127
Hin6I GCGC 2 cut(s) 201, 621
HinP1I GCGC 2 cut(s) 201, 621
HincII GTYRAC 2 cut(s) 316, 1075
HindII GTYRAC 2 cut(s) 316, 1075
HindIII AAGCTT 3 cut(s) 180, 1002, 1154
HinfI GANTC 2 cut(s) 406, 644
HpaI GTTAAC 2 cut(s) 316, 1075
HpaII CCGG 3 cut(s) 90, 410, 824
HphI GGTGA 2 cut(s) 653, 857
Hpy166II GTNNAC 3 cut(s) 139, 316, 1075
Hpy188I TCNGA 8 cut(s) 19, 220, 263, 283, 405, 836, 995, 1171
Hpy188III TCNNGA 4 cut(s) 395, 420, 648, 1124
Hpy8I GTNNAC 3 cut(s) 139, 316, 1075
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 7 cut(s) 139, 493, 505, 521, 580, 926, 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 464, 932
HpyF3I CTNAG 6 cut(s) 260, 384, 524, 975, 1032, 1115
HpySE526I ACGT 1 cut(s) 427
Hsp92I GRCGYC 1 cut(s) 751
Hsp92II CATG 5 cut(s) 398, 758, 910, 961, 1127
HspAI GCGC 2 cut(s) 201, 621
Ksp22I TGATCA 2 cut(s) 249, 330
KspAI GTTAAC 2 cut(s) 316, 1075
Kzo9I GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
LmnI GCTCC 2 cut(s) 127, 406
Lsp1109I GCAGC 1 cut(s) 979
LweI GCATC 6 cut(s) 211, 388, 567, 954, 999, 1132
MabI ACCWGGT 2 cut(s) 544, 637
MaeI CTAG 2 cut(s) 375, 1130
MaeII ACGT 1 cut(s) 427
MalI GATC 6 cut(s) 251, 332, 424, 652, 852, 1122
MboI GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
MboII GAAGA 2 cut(s) 860, 1172
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 2 cut(s) 141, 476
MluCI AATT 3 cut(s) 115, 159, 1020
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 1 cut(s) 649
MroXI GAANNNNTTC 1 cut(s) 103
MseI TTAA 7 cut(s) 207, 315, 626, 774, 810, 1043, 1074
MslI CAYNNNNRTG 1 cut(s) 962
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 3 cut(s) 90, 410, 824
MspR9I CCNGG 9 cut(s) 91, 232, 546, 614, 639, 793, 825, 855, 882
MvaI CCWGG 7 cut(s) 232, 546, 614, 639, 793, 855, 882
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 3 cut(s) 209, 464, 932
NciI CCSGG 2 cut(s) 91, 825
NcoI CCATGG 1 cut(s) 906
NdeI CATATG 1 cut(s) 655
NdeII GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
NlaIII CATG 5 cut(s) 398, 758, 910, 961, 1127
NlaIV GGNNCC 2 cut(s) 474, 611
PagI TCATGA 2 cut(s) 394, 1123
PceI AGGCCT 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 103
PfeI GAWTC 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 823
PkrI GCNGC 1 cut(s) 969
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 1 cut(s) 536
PsiI TTATAA 1 cut(s) 725
Psp5II RGGWCCY 1 cut(s) 536
Psp6I CCWGG 7 cut(s) 230, 544, 612, 637, 791, 853, 880
PspFI CCCAGC 1 cut(s) 1084
PspGI CCWGG 7 cut(s) 230, 544, 612, 637, 791, 853, 880
PspN4I GGNNCC 2 cut(s) 474, 611
PspOMI GGGCCC 1 cut(s) 472
PspPI GGNCC 6 cut(s) 472, 473, 536, 548, 609, 920
PspPPI RGGWCCY 1 cut(s) 536
PsuI RGATCY 1 cut(s) 850
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 2 cut(s) 31, 584
RsaNI GTAC 2 cut(s) 30, 583
RseI CAYNNNNRTG 1 cut(s) 962
SaqAI TTAA 7 cut(s) 207, 315, 626, 774, 810, 1043, 1074
SatI GCNGC 1 cut(s) 968
Sau3AI GATC 6 cut(s) 249, 330, 422, 650, 850, 1120
Sau96I GGNCC 6 cut(s) 472, 473, 536, 548, 609, 920
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 9 cut(s) 91, 232, 546, 614, 639, 793, 825, 855, 882
SduI GDGCHC 2 cut(s) 141, 476
SexAI ACCWGGT 2 cut(s) 544, 637
SfaNI GCATC 6 cut(s) 211, 388, 567, 954, 999, 1132
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 3 cut(s) 536, 548, 920
SmiMI CAYNNNNRTG 1 cut(s) 962
Sse9I AATT 3 cut(s) 115, 159, 1020
SseBI AGGCCT 1 cut(s) 103
SsiI CCGC 3 cut(s) 199, 935, 1182
SspI AATATT 1 cut(s) 1189
SspMI CTAG 2 cut(s) 375, 1130
StuI AGGCCT 1 cut(s) 103
StyD4I CCNGG 9 cut(s) 89, 230, 544, 612, 637, 791, 823, 853, 880
StyI CCWWGG 1 cut(s) 906
TaiI ACGT 1 cut(s) 430
TaqI TCGA 4 cut(s) 191, 274, 575, 1017
TasI AATT 3 cut(s) 115, 159, 1020
TfiI GAWTC 1 cut(s) 644
Tru1I TTAA 7 cut(s) 207, 315, 626, 774, 810, 1043, 1074
Tru9I TTAA 7 cut(s) 207, 315, 626, 774, 810, 1043, 1074
TscAI CASTG 2 cut(s) 259, 1036
TseI GCWGC 1 cut(s) 967
TspDTI ATGAA 7 cut(s) 152, 159, 339, 383, 648, 801, 1140
TspRI CASTG 2 cut(s) 259, 1036
VneI GTGCAC 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 536, 548, 920
XagI CCTNNNNNAGG 2 cut(s) 543, 885
XapI RAATTY 2 cut(s) 159, 1020
XmnI GAANNNNTTC 1 cut(s) 103
XspI CTAG 2 cut(s) 375, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.