Rh5CG567600

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007058
Physical Location & Seq
Forward (+)
80350831 .. 80356275
5445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG567600.1

Sequence Viewer

Length: 1104 bp
ATGCATGGTGATATGGAAACACAGATCCACCACATTGAGCAAGAAGCATACTGTTCAGTCCTGCGGGCATTTAAAGCCCAATCTGATGCCATCACCTGGGAGAAGGAAAGTTTAATAACGGAACTTAGAAAGGAGCTGAGAGTATCGGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCGAAGAATAAGGGAGTGGAGAAAAACCAGTGGGCCTCAGCCGGGTACACATAATGCCGCTCAGCCTGTTCATGACCCCATACCTAGTCCAACTGTTTCGGCATCACGTAAGAAACAGAAAACATCACAGTCTGTAGCTTCTTTATCACATGGTGGTGCTCCATCTCCAGTGCTGCCTCCATCCATGCAACCTTCCTCATCAGCCTTGAGACGTGGTCCTCCTCCAGGAGCTCGGAGCAAGAAGTCAAAATCATCCACACAGTTCCCTTCTACAAGTATTACCGGAAGGCCCCAAGCCCCAAACCGAGGTTCTTCAGGTGGCTTTGTGGCAACTGAGCCTGCTGAGGCAACAACTTATGATCCATTAATAGGAAGAAAAGTATGGACAAGGTGGCCCGCGGACAACCATTTCTATGAGGCTGTAATAACTGATTACAACCATATTCAGGGCCGACATGCTCTGGTTTATGATATCAATACAGCGGATGAAACATGGGAATGGGTCAACCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGGTGATGATCCTGGGTTTTCTCGTAGGGGTGGTCGACCTGGATCTGGCCGAGGGACTAAGAAGTCCATGGGACGTGGTGGTGCAGTTTCAGGTGCAGGAAGGGGTAGAGGGACCACAAAAGGTCAGCCTAAGAAGGATTTGCCTCCACAACAAAATGGCATTGGCAGGAAGGCCATGAGCGACATTGAAATACTTCATACAGACTCGTTGATTAAAGAGGTGGAAAAGGTCTTCAGTGCTAGCCATCCTGACCCGATGGAGATTGAGAAAGCTAAGAAAGTTCTCAAAGAGCATGAACAGGCATTGATCGATGCAATTGCAACGCTTGAAGATGCATCAGATGGTGAAAGTGGTAATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

367

Amino Acids

40.26

Weight (kDa)

8.47

Isoelectric Point (pI)

56.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 7 - 75 2.1e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 802
AccB7I CCANNNNNTGG 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 775
AccII CGCG 1 cut(s) 596
AciI CCGC 5 cut(s) 64, 255, 594, 596, 680
AclWI GGATC 5 cut(s) 19, 190, 551, 743, 790
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 495, 744, 958
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 1 cut(s) 244
AfiI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
AgsI TTSAA 2 cut(s) 929, 1070
AjiI CACGTC 2 cut(s) 410, 815
AjnI CCWGG 5 cut(s) 95, 173, 421, 751, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AluBI AGCT 4 cut(s) 136, 335, 428, 1013
AluI AGCT 4 cut(s) 136, 335, 428, 1013
Alw21I GWGCWC 2 cut(s) 358, 430
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 5 cut(s) 19, 190, 551, 743, 790
AoxI GGCC 6 cut(s) 230, 485, 590, 646, 787, 912
ApeKI GCWGC 1 cut(s) 370
AseI ATTAAT 1 cut(s) 563
Asp700I GAANNNNTTC 2 cut(s) 729, 933
AspS9I GGNCC 6 cut(s) 230, 413, 486, 591, 646, 852
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 4 cut(s) 20, 85, 755, 1097
AsuNHI GCTAGC 1 cut(s) 980
AvaII GGWCC 2 cut(s) 413, 852
BanII GRGCYC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 964
Bbv12I GWGCWC 2 cut(s) 358, 430
BbvCI CCTCAGC 2 cut(s) 234, 540
BbvI GCAGC 1 cut(s) 357
BccI CCATC 6 cut(s) 98, 367, 385, 991, 993, 1076
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 5 cut(s) 97, 175, 423, 753, 780
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 282, 981
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 2 cut(s) 255, 371
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 256, 372
Bme1390I CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
Bme18I GGWCC 2 cut(s) 413, 852
BmgBI CACGTC 2 cut(s) 410, 815
BmgT120I GGNCC 6 cut(s) 230, 413, 486, 591, 646, 852
BmiI GGNNCC 2 cut(s) 488, 853
BmrFI CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
BmsI GCATC 5 cut(s) 76, 308, 1042, 1063, 1085
BmtI GCTAGC 1 cut(s) 984
BpiI GAAGAC 1 cut(s) 964
BpmI CTGGAG 2 cut(s) 348, 405
Bpu10I CCTNAGC 2 cut(s) 234, 540
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 1050
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 7 cut(s) 96, 174, 502, 594, 752, 790, 807
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
Bse1I ACTGG 2 cut(s) 225, 365
BseBI CCWGG 5 cut(s) 97, 175, 423, 753, 780
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 7 cut(s) 96, 174, 502, 594, 752, 790, 807
BseGI GGATG 5 cut(s) 154, 377, 449, 688, 985
BseLI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
BseMII CTCAG 5 cut(s) 128, 248, 272, 522, 531
BseNI ACTGG 2 cut(s) 225, 365
BseRI GAGGAG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 357
BsgI GTGCAG 2 cut(s) 843, 855
Bsh1236I CGCG 1 cut(s) 596
BshFI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BshVI ATCGAT 1 cut(s) 1050
BsiHKAI GWGCWC 2 cut(s) 358, 430
BsiSI CCGG 2 cut(s) 239, 480
BslFI GGGAC 3 cut(s) 808, 825, 865
BslI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
BsmAI GTCTC 1 cut(s) 400
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 3 cut(s) 808, 825, 865
BsnI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
Bsp1286I GDGCHC 2 cut(s) 358, 430
Bsp143I GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 5 cut(s) 64, 255, 594, 596, 680
BspANI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BspCNI CTCAG 5 cut(s) 129, 247, 271, 523, 532
BspDI ATCGAT 1 cut(s) 1050
BspFNI CGCG 1 cut(s) 596
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 2 cut(s) 488, 853
BspOI GCTAGC 1 cut(s) 984
BspPI GGATC 5 cut(s) 19, 190, 551, 743, 790
BsrBI CCGCTC 1 cut(s) 257
BsrI ACTGG 2 cut(s) 225, 365
BssECI CCNNGG 7 cut(s) 96, 174, 502, 594, 752, 790, 807
BssMI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
BssT1I CCWWGG 1 cut(s) 807
Bst2UI CCWGG 5 cut(s) 97, 175, 423, 753, 780
Bst4CI ACNGT 4 cut(s) 53, 292, 327, 459
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 66, 537, 594, 982
BstDEI CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
BstDSI CCRYGG 2 cut(s) 594, 807
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 5 cut(s) 154, 377, 449, 688, 985
BstFNI CGCG 1 cut(s) 596
BstKTI GATC 6 cut(s) 27, 198, 559, 751, 785, 1050
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 5 cut(s) 97, 175, 423, 753, 780
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 95, 173, 238, 421, 751, 778
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 1 cut(s) 596
BstV1I GCAGC 1 cut(s) 357
BstV2I GAAGAC 1 cut(s) 964
BstX2I RGATCY 2 cut(s) 24, 782
BstYI RGATCY 2 cut(s) 24, 782
Bsu15I ATCGAT 1 cut(s) 1050
BsuRI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BsuTUI ATCGAT 1 cut(s) 1050
BtgI CCRYGG 2 cut(s) 594, 807
BtrI CACGTC 2 cut(s) 410, 815
BtsCI GGATG 5 cut(s) 154, 377, 449, 688, 985
BtsIMutI CAGTG 3 cut(s) 232, 372, 982
Cac8I GCNNGC 4 cut(s) 66, 537, 594, 982
CciI TCATGA 1 cut(s) 268
Cfr13I GGNCC 6 cut(s) 230, 413, 486, 591, 646, 852
Cfr42I CCGCGG 1 cut(s) 597
ClaI ATCGAT 1 cut(s) 1050
Csp6I GTAC 1 cut(s) 243
CviQI GTAC 1 cut(s) 243
DdeI CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
DpnI GATC 6 cut(s) 26, 197, 558, 750, 784, 1049
DpnII GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 350
DrdI GACNNNNNNGTC 1 cut(s) 802
DseDI GACNNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 787
Ecl136II GAGCTC 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 430
Eco32I GATATC 2 cut(s) 670, 717
Eco47I GGWCC 2 cut(s) 413, 852
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 3 cut(s) 495, 744, 958
EcoICRI GAGCTC 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 486
EcoRII CCWGG 5 cut(s) 95, 173, 421, 751, 778
EcoRV GATATC 2 cut(s) 670, 717
EcoT14I CCWWGG 1 cut(s) 807
EcoT22I ATGCAT 2 cut(s) 6, 1078
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 807
Esp3I CGTCTC 1 cut(s) 400
FaqI GGGAC 3 cut(s) 808, 825, 865
FauI CCCGC 2 cut(s) 57, 601
FblI GTMKAC 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 255, 371
FokI GGATG 5 cut(s) 161, 364, 436, 695, 972
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 2 cut(s) 255, 371
FspBI CTAG 2 cut(s) 282, 981
GluI GCNGC 2 cut(s) 255, 371
GsuI CTGGAG 2 cut(s) 348, 405
HaeIII GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
HapII CCGG 2 cut(s) 239, 480
HincII GTYRAC 2 cut(s) 703, 776
HindII GTYRAC 2 cut(s) 703, 776
HinfI GANTC 1 cut(s) 944
HpaII CCGG 2 cut(s) 239, 480
HphI GGTGA 4 cut(s) 20, 85, 755, 1097
Hpy166II GTNNAC 3 cut(s) 245, 703, 776
Hpy188I TCNGA 5 cut(s) 85, 148, 200, 432, 1081
Hpy188III TCNNGA 3 cut(s) 269, 722, 989
Hpy8I GTNNAC 3 cut(s) 245, 703, 776
HpyAV CCTTC 8 cut(s) 97, 399, 474, 477, 734, 834, 868, 904
HpyCH4III ACNGT 4 cut(s) 53, 292, 327, 459
HpyCH4IV ACGT 3 cut(s) 304, 409, 814
HpyCH4V TGCA 7 cut(s) 4, 385, 824, 836, 1055, 1061, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
HpySE526I ACGT 3 cut(s) 304, 409, 814
KspI CCGCGG 1 cut(s) 597
Kzo9I GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
LmnI GCTCC 4 cut(s) 133, 361, 425, 432
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 5 cut(s) 76, 308, 1042, 1063, 1085
MaeI CTAG 2 cut(s) 282, 981
MaeII ACGT 3 cut(s) 304, 409, 814
MalI GATC 6 cut(s) 26, 197, 558, 750, 784, 1049
MbiI CCGCTC 1 cut(s) 257
MboI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
MboII GAAGA 6 cut(s) 213, 501, 582, 737, 964, 1082
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 2 cut(s) 24, 782
MhlI GDGCHC 2 cut(s) 358, 430
MluCI AATT 1 cut(s) 1056
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 1 cut(s) 311
Mph1103I ATGCAT 2 cut(s) 6, 1078
MroXI GAANNNNTTC 2 cut(s) 729, 933
MseI TTAA 4 cut(s) 72, 113, 563, 954
MslI CAYNNNNRTG 3 cut(s) 351, 609, 694
MspA1I CMGCKG 2 cut(s) 596, 680
MspI CCGG 2 cut(s) 239, 480
MspR9I CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
MunI CAATTG 1 cut(s) 1056
MvaI CCWGG 5 cut(s) 97, 175, 423, 753, 780
MvnI CGCG 1 cut(s) 596
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 807
NdeII GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
NheI GCTAGC 1 cut(s) 980
NlaIV GGNNCC 2 cut(s) 488, 853
NmeAIII GCCGAG 1 cut(s) 815
NsiI ATGCAT 2 cut(s) 6, 1078
NspI RCATGY 1 cut(s) 656
PagI TCATGA 1 cut(s) 268
PdmI GAANNNNTTC 2 cut(s) 729, 933
PflFI GACNNNGTC 1 cut(s) 411
PflMI CCANNNNNTGG 1 cut(s) 96
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 2 cut(s) 256, 372
PleI GAGTC 1 cut(s) 938
PpsI GAGTC 1 cut(s) 938
Ppu21I YACGTR 1 cut(s) 305
PshBI ATTAAT 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 430
Psp6I CCWGG 5 cut(s) 95, 173, 421, 751, 778
PspGI CCWGG 5 cut(s) 95, 173, 421, 751, 778
PspN4I GGNNCC 2 cut(s) 488, 853
PspPI GGNCC 6 cut(s) 230, 413, 486, 591, 646, 852
PsuI RGATCY 2 cut(s) 24, 782
PsyI GACNNNGTC 1 cut(s) 411
RsaI GTAC 1 cut(s) 244
RsaNI GTAC 1 cut(s) 243
RseI CAYNNNNRTG 3 cut(s) 351, 609, 694
SacI GAGCTC 1 cut(s) 430
SacII CCGCGG 1 cut(s) 597
SalI GTCGAC 1 cut(s) 774
SaqAI TTAA 4 cut(s) 72, 113, 563, 954
SatI GCNGC 2 cut(s) 255, 371
Sau3AI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Sau96I GGNCC 6 cut(s) 230, 413, 486, 591, 646, 852
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
SduI GDGCHC 2 cut(s) 358, 430
SfaNI GCATC 5 cut(s) 76, 308, 1042, 1063, 1085
SfcI CTRYAG 1 cut(s) 330
Sfr303I CCGCGG 1 cut(s) 597
SgrBI CCGCGG 1 cut(s) 597
SinI GGWCC 2 cut(s) 413, 852
SmiMI CAYNNNNRTG 3 cut(s) 351, 609, 694
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 1 cut(s) 1056
SsiI CCGC 5 cut(s) 64, 255, 594, 596, 680
SspMI CTAG 2 cut(s) 282, 981
SstI GAGCTC 1 cut(s) 430
StyD4I CCNGG 6 cut(s) 95, 173, 238, 421, 751, 778
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 4 cut(s) 53, 292, 327, 459
TaiI ACGT 3 cut(s) 307, 412, 817
TaqI TCGA 2 cut(s) 775, 1050
TasI AATT 1 cut(s) 1056
TauI GCSGC 1 cut(s) 257
Tru1I TTAA 4 cut(s) 72, 113, 563, 954
Tru9I TTAA 4 cut(s) 72, 113, 563, 954
TscAI CASTG 3 cut(s) 232, 372, 982
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 4 cut(s) 257, 699, 926, 1050
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 3 cut(s) 232, 372, 982
Tth111I GACNNNGTC 1 cut(s) 411
Van91I CCANNNNNTGG 1 cut(s) 96
VpaK11BI GGWCC 2 cut(s) 413, 852
VspI ATTAAT 1 cut(s) 563
XagI CCTNNNNNAGG 1 cut(s) 420
XceI RCATGY 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 775
XmnI GAANNNNTTC 2 cut(s) 729, 933
XspI CTAG 2 cut(s) 282, 981
Zsp2I ATGCAT 2 cut(s) 6, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.