MD11G1019600.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007058
Physical Location & Seq
Reverse (-)
1676836 .. 1682040
5205 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1019600.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGACTACGAGCTCTCTGATAGCAGTGGAACGGACGATGACTTTCCGCCGTCTCATCAAAATAGGTTTCAAAGAGGAGGCCGAACTTCTGGGAATGGAAGATCTGCAGCCGTCAGTTCTGCTCCATTACCTAGGATGCATGGTGACATGGAAACCCAGATTCACCGCATTGAGCAAGAAGCGTACTGTTCACTTCTAAGGGCATTTAGTGCTCAATCTGATGCCATCACCTGGGATAAGGAAACTTTAATGACAAATCTTAGAAAGGAGCTGAGAGTATCTGATGAGGAGCACAGAGAACTTTTGTCCAGGGTCCATGCCGATGACATGATCCAGAGAATAAGGCAATGGAGAAAAAGTGGGCTTCAACCTGGTACACTTAATGCTGCTCAGCCTGTTCATGACCCCCTACCTAGTCCAACTGTTTCAGCATCACGGAAGAAACAGAAAACATCACAGTCCTTATCCCTCGGTGCTCCAACTCCTGCACTGCCTCCATCCATGCAACCATCCTCTTCAGCCTTGAGACGAGGTCCTCCTCCAGGACCTCGGAACAAGAAACCAAAATCTTCCACACAGTACCCTTCCGCAAATATTCCTCTAAGGCCCCAGGCCGCTAATCGAGGTCCCGCTGGTGCCTTTTTGGAGAGTGAGCCTGCTGAGGGAGAAACTAGTAATCCGTTAGTAGGAAGAAAGGTTTGGACAAGGTGGCCTGAAGACAACCACTTCTATGAGGCTGTTATAACTAATTTCAACCGTGCTGAGGGTCTACATGCTCTGGTTTATGATATTGGTACAGTGGATGAGACATGGGAATGGGTCAATCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGCTGATGATCCTGGGATTTCTCGTAAAGGTGGTCGACCTGGCCCTGGACCTGGCCGAGGGAACAAAAAGTCCATGGCACGTGGCGGTTTAGTTTCAGGTGCAGGAAGAGGTAGAGGGACTGCAAAGGGTTCCAAGAAGGATTTGCCCCCGCAGCTAAATGGCATTGGCAGGAAGGCAATGAGCGATATTGAAATACTTCATACAGATTCGTTGATTAAAGAGGTGGAAAGGGTTTTCAGAGCCAGCCATCCCGACCCCATGGAGATTGAGAAAGCCAAGAAAGTTCTCAAAGAGCATGAACAGGCACTGGTCGATGCAATTACAAGACTCGAAGATGCATCTGACGTTGAAAGCGATGAACATACATTCTCTCAAAGACAATCACTAGACCAAGAACGAGGATGGAGAAAGCGGCAGTATGACGAGATGGCTGAAGGTCGGGCAGTTGACGGCTCAAATGGCAATAAAATGACAAGGGAGGGCAGAATTGCATCTGATGACCAACGTTTTGAGGGCAACGACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

463

Amino Acids

51.22

Weight (kDa)

6.9

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 52 - 120 4.7e-30 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 743
AccB1I GGYRCC 1 cut(s) 635
AccB7I CCANNNNNTGG 1 cut(s) 231
AccI GTMKAC 2 cut(s) 769, 895
AciI CCGC 8 cut(s) 47, 166, 588, 615, 630, 945, 1010, 1273
AclI AACGTT 1 cut(s) 1366
AclWI GGATC 2 cut(s) 325, 863
AcoI YGGCCR 1 cut(s) 913
AcuI CTGAAG 4 cut(s) 501, 735, 864, 1314
AcvI CACGTG 1 cut(s) 941
AfaI GTAC 4 cut(s) 185, 376, 581, 796
AfiI CCNNNNNNNGG 8 cut(s) 231, 542, 662, 686, 763, 905, 911, 917
AgsI TTSAA 5 cut(s) 71, 368, 754, 1052, 1211
AhlI ACTAGT 1 cut(s) 671
AjnI CCWGG 9 cut(s) 230, 308, 370, 541, 609, 871, 898, 904, 910
AjuI GAANNNNNNNTTGG 2 cut(s) 682, 714
AloI GAACNNNNNNTCC 2 cut(s) 914, 946
AluBI AGCT 4 cut(s) 13, 271, 863, 1015
AluI AGCT 4 cut(s) 13, 271, 863, 1015
Alw21I GWGCWC 4 cut(s) 15, 214, 294, 478
Alw26I GTCTC 3 cut(s) 57, 520, 800
AlwI GGATC 2 cut(s) 325, 863
AlwNI CAGNNNCTG 1 cut(s) 1168
AoxI GGCC 6 cut(s) 79, 605, 612, 710, 901, 913
ApeKI GCWGC 3 cut(s) 107, 386, 1012
ArsI GACNNNNNNTTYG 2 cut(s) 1352, 1384
Asp700I GAANNNNTTC 2 cut(s) 849, 1056
AspA2I CCTAGG 1 cut(s) 131
AspS9I GGNCC 7 cut(s) 313, 533, 545, 606, 626, 902, 908
AsuHPI GGTGA 3 cut(s) 155, 155, 220
AvaII GGWCC 5 cut(s) 313, 533, 545, 626, 908
AvrII CCTAGG 1 cut(s) 131
BanI GGYRCC 1 cut(s) 635
BanII GRGCYC 1 cut(s) 15
BbrPI CACGTG 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 4 cut(s) 15, 214, 294, 478
BbvCI CCTCAGC 2 cut(s) 660, 762
BbvI GCAGC 3 cut(s) 119, 373, 1024
BccI CCATC 6 cut(s) 233, 505, 517, 1116, 1257, 1282
BceAI ACGGC 3 cut(s) 34, 95, 1327
BciT130I CCWGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
BcoDI GTCTC 3 cut(s) 57, 520, 800
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 4 cut(s) 132, 414, 672, 1247
BfmI CTRYAG 1 cut(s) 105
BglII AGATCT 1 cut(s) 101
BisI GCNGC 5 cut(s) 108, 387, 615, 1013, 1274
BlnI CCTAGG 1 cut(s) 131
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 5 cut(s) 109, 388, 616, 1014, 1275
Bme1390I CCNGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
Bme18I GGWCC 5 cut(s) 313, 533, 545, 626, 908
BmgT120I GGNCC 7 cut(s) 313, 533, 545, 606, 626, 902, 908
BmiI GGNNCC 5 cut(s) 314, 608, 628, 637, 991
BmrFI CCNGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
BmsI GCATC 7 cut(s) 126, 211, 440, 1165, 1186, 1208, 1361
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 1 cut(s) 525
Bpu10I CCTNAGC 2 cut(s) 660, 762
Bpu1102I GCTNAGC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 544
BsaAI YACGTR 1 cut(s) 941
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bsc4I CCNNNNNNNGG 8 cut(s) 231, 542, 662, 686, 763, 905, 911, 917
Bse1I ACTGG 1 cut(s) 1173
Bse3DI GCAATG 2 cut(s) 353, 1044
BseBI CCWGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
BseGI GGATG 6 cut(s) 141, 497, 509, 808, 1108, 1268
BseLI CCNNNNNNNGG 8 cut(s) 231, 542, 662, 686, 763, 905, 911, 917
BseMI GCAATG 2 cut(s) 353, 1044
BseMII CTCAG 4 cut(s) 263, 404, 651, 753
BseNI ACTGG 1 cut(s) 1173
BseRI GAGGAG 3 cut(s) 90, 302, 528
BseXI GCAGC 3 cut(s) 119, 373, 1024
BsgI GTGCAG 2 cut(s) 471, 981
BshFI GGCC 6 cut(s) 81, 607, 614, 712, 903, 915
BshNI GGYRCC 1 cut(s) 635
BsiHKAI GWGCWC 4 cut(s) 15, 214, 294, 478
BslFI GGGAC 2 cut(s) 612, 991
BslI CCNNNNNNNGG 8 cut(s) 231, 542, 662, 686, 763, 905, 911, 917
BsmAI GTCTC 3 cut(s) 57, 520, 800
BsmBI CGTCTC 2 cut(s) 57, 520
BsmFI GGGAC 2 cut(s) 612, 991
BsnI GGCC 6 cut(s) 81, 607, 614, 712, 903, 915
Bsp1286I GDGCHC 4 cut(s) 15, 214, 294, 478
Bsp143I GATC 3 cut(s) 101, 330, 868
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 2 cut(s) 933, 1119
BspACI CCGC 8 cut(s) 47, 166, 588, 615, 630, 945, 1010, 1273
BspANI GGCC 6 cut(s) 81, 607, 614, 712, 903, 915
BspCNI CTCAG 4 cut(s) 264, 403, 652, 754
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 5 cut(s) 314, 608, 628, 637, 991
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 2 cut(s) 325, 863
BspT107I GGYRCC 1 cut(s) 635
BsrDI GCAATG 2 cut(s) 353, 1044
BsrI ACTGG 1 cut(s) 1173
BssMI GATC 3 cut(s) 101, 330, 868
BssT1I CCWWGG 3 cut(s) 131, 933, 1119
Bst2UI CCWGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
Bst4CI ACNGT 6 cut(s) 188, 424, 459, 579, 758, 799
Bst6I CTCTTC 2 cut(s) 520, 961
BstAPI GCANNNNNTGC 1 cut(s) 209
BstBAI YACGTR 1 cut(s) 941
BstC8I GCNNGC 2 cut(s) 657, 1105
BstDEI CTNAG 7 cut(s) 197, 260, 272, 390, 602, 660, 762
BstDSI CCRYGG 2 cut(s) 933, 1119
BstENI CCTNNNNNAGG 2 cut(s) 540, 915
BstF5I GGATG 6 cut(s) 141, 497, 509, 808, 1108, 1268
BstKTI GATC 3 cut(s) 104, 333, 871
BstMAI GTCTC 3 cut(s) 57, 520, 800
BstMBI GATC 3 cut(s) 101, 330, 868
BstMWI GCNNNNNNNGC 3 cut(s) 209, 1012, 1320
BstNI CCWGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
BstNSI RCATGY 1 cut(s) 776
BstSCI CCNGG 9 cut(s) 230, 308, 370, 541, 609, 871, 898, 904, 910
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 3 cut(s) 119, 373, 1024
BstV2I GAAGAC 1 cut(s) 723
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 6 cut(s) 81, 607, 614, 712, 903, 915
BtgI CCRYGG 2 cut(s) 933, 1119
BtgZI GCGATG 1 cut(s) 1230
BtsCI GGATG 6 cut(s) 141, 497, 509, 808, 1108, 1268
BtsI GCAGTG 2 cut(s) 31, 488
BtsIMutI CAGTG 4 cut(s) 31, 488, 804, 1166
Cac8I GCNNGC 2 cut(s) 657, 1105
CaiI CAGNNNCTG 1 cut(s) 1168
CciI TCATGA 1 cut(s) 400
Cfr13I GGNCC 7 cut(s) 313, 533, 545, 606, 626, 902, 908
CsiI ACCWGGT 1 cut(s) 370
Csp6I GTAC 4 cut(s) 184, 375, 580, 795
CviQI GTAC 4 cut(s) 184, 375, 580, 795
DdeI CTNAG 7 cut(s) 197, 260, 272, 390, 602, 660, 762
DpnI GATC 3 cut(s) 103, 332, 870
DpnII GATC 3 cut(s) 101, 330, 868
EaeI YGGCCR 1 cut(s) 913
Eam1104I CTCTTC 2 cut(s) 520, 961
EarI CTCTTC 2 cut(s) 520, 961
EciI GGCGGA 1 cut(s) 36
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 3 cut(s) 131, 933, 1119
Eco24I GRGCYC 1 cut(s) 15
Eco32I GATATC 1 cut(s) 837
Eco47I GGWCC 5 cut(s) 313, 533, 545, 626, 908
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 4 cut(s) 501, 735, 864, 1314
Eco72I CACGTG 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 2 cut(s) 540, 915
EcoO109I RGGNCCY 4 cut(s) 533, 545, 606, 626
EcoRII CCWGG 9 cut(s) 230, 308, 370, 541, 609, 871, 898, 904, 910
EcoRV GATATC 1 cut(s) 837
EcoT14I CCWWGG 3 cut(s) 131, 933, 1119
EcoT22I ATGCAT 2 cut(s) 141, 1201
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 3 cut(s) 131, 933, 1119
Esp3I CGTCTC 2 cut(s) 57, 520
FaqI GGGAC 2 cut(s) 612, 991
FauI CCCGC 2 cut(s) 637, 1017
FauNDI CATATG 1 cut(s) 1385
FblI GTMKAC 2 cut(s) 769, 895
Fnu4HI GCNGC 5 cut(s) 108, 387, 615, 1013, 1274
FokI GGATG 6 cut(s) 148, 484, 496, 815, 1095, 1275
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 5 cut(s) 108, 387, 615, 1013, 1274
FspBI CTAG 4 cut(s) 132, 414, 672, 1247
GluI GCNGC 5 cut(s) 108, 387, 615, 1013, 1274
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 6 cut(s) 81, 607, 614, 712, 903, 915
HincII GTYRAC 2 cut(s) 896, 1309
HindII GTYRAC 2 cut(s) 896, 1309
HinfI GANTC 3 cut(s) 160, 1067, 1188
HphI GGTGA 3 cut(s) 155, 155, 220
Hpy166II GTNNAC 5 cut(s) 191, 377, 770, 896, 1309
Hpy188I TCNGA 7 cut(s) 19, 220, 283, 552, 1100, 1204, 1357
Hpy188III TCNNGA 4 cut(s) 334, 401, 842, 1112
Hpy8I GTNNAC 5 cut(s) 191, 377, 770, 896, 1309
HpyAV CCTTC 4 cut(s) 594, 991, 1027, 1289
HpyCH4III ACNGT 6 cut(s) 188, 424, 459, 579, 758, 799
HpyCH4IV ACGT 3 cut(s) 940, 1206, 1366
HpyCH4V TGCA 9 cut(s) 107, 139, 488, 505, 962, 983, 1178, 1199, 1352
HpyF10VI GCNNNNNNNGC 3 cut(s) 209, 1012, 1320
HpyF3I CTNAG 7 cut(s) 197, 260, 272, 390, 602, 660, 762
HpySE526I ACGT 3 cut(s) 940, 1206, 1366
Kzo9I GATC 3 cut(s) 101, 330, 868
LmnI GCTCC 4 cut(s) 127, 268, 289, 481
Lsp1109I GCAGC 3 cut(s) 119, 373, 1024
LweI GCATC 7 cut(s) 126, 211, 440, 1165, 1186, 1208, 1361
MabI ACCWGGT 1 cut(s) 370
MaeI CTAG 4 cut(s) 132, 414, 672, 1247
MaeII ACGT 3 cut(s) 940, 1206, 1366
MaeIII GTNAC 1 cut(s) 143
MalI GATC 3 cut(s) 103, 332, 870
MboI GATC 3 cut(s) 101, 330, 868
MboII GAAGA 9 cut(s) 111, 451, 507, 561, 702, 728, 857, 978, 1205
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 4 cut(s) 15, 214, 294, 478
MluCI AATT 3 cut(s) 748, 1179, 1347
MlyI GAGTC 1 cut(s) 1182
MmeI TCCRAC 2 cut(s) 443, 503
Mph1103I ATGCAT 2 cut(s) 141, 1201
MroXI GAANNNNTTC 2 cut(s) 849, 1056
MseI TTAA 3 cut(s) 248, 381, 1077
MslI CAYNNNNRTG 3 cut(s) 321, 729, 814
MspA1I CMGCKG 1 cut(s) 632
MspR9I CCNGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
MvaI CCWGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
MwoI GCNNNNNNNGC 3 cut(s) 209, 1012, 1320
NcoI CCATGG 2 cut(s) 933, 1119
NdeI CATATG 1 cut(s) 1385
NdeII GATC 3 cut(s) 101, 330, 868
NlaIV GGNNCC 5 cut(s) 314, 608, 628, 637, 991
NmeAIII GCCGAG 1 cut(s) 941
NmuCI GTSAC 1 cut(s) 143
NsiI ATGCAT 2 cut(s) 141, 1201
NspI RCATGY 1 cut(s) 776
PagI TCATGA 1 cut(s) 400
PcsI WCGNNNNNNNCGW 1 cut(s) 1212
PdmI GAANNNNTTC 2 cut(s) 849, 1056
PfeI GAWTC 2 cut(s) 160, 1067
PflFI GACNNNGTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 5 cut(s) 109, 388, 616, 1014, 1275
PleI GAGTC 1 cut(s) 1182
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
PpsI GAGTC 1 cut(s) 1182
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 3 cut(s) 533, 545, 626
PsiI TTATAA 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 15
Psp1406I AACGTT 1 cut(s) 1366
Psp5II RGGWCCY 3 cut(s) 533, 545, 626
Psp6I CCWGG 9 cut(s) 230, 308, 370, 541, 609, 871, 898, 904, 910
PspCI CACGTG 1 cut(s) 941
PspGI CCWGG 9 cut(s) 230, 308, 370, 541, 609, 871, 898, 904, 910
PspN4I GGNNCC 5 cut(s) 314, 608, 628, 637, 991
PspPI GGNCC 7 cut(s) 313, 533, 545, 606, 626, 902, 908
PspPPI RGGWCCY 3 cut(s) 533, 545, 626
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 1168
PsuI RGATCY 1 cut(s) 101
PsyI GACNNNGTC 1 cut(s) 531
RsaI GTAC 4 cut(s) 185, 376, 581, 796
RsaNI GTAC 4 cut(s) 184, 375, 580, 795
RseI CAYNNNNRTG 3 cut(s) 321, 729, 814
SacI GAGCTC 1 cut(s) 15
SalI GTCGAC 1 cut(s) 894
SaqAI TTAA 3 cut(s) 248, 381, 1077
SatI GCNGC 5 cut(s) 108, 387, 615, 1013, 1274
Sau3AI GATC 3 cut(s) 101, 330, 868
Sau96I GGNCC 7 cut(s) 313, 533, 545, 606, 626, 902, 908
SchI GAGTC 1 cut(s) 1182
ScrFI CCNGG 9 cut(s) 232, 310, 372, 543, 611, 873, 900, 906, 912
SduI GDGCHC 4 cut(s) 15, 214, 294, 478
SexAI ACCWGGT 1 cut(s) 370
SfaNI GCATC 7 cut(s) 126, 211, 440, 1165, 1186, 1208, 1361
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 5 cut(s) 313, 533, 545, 626, 908
SmiMI CAYNNNNRTG 3 cut(s) 321, 729, 814
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 671
Sse9I AATT 3 cut(s) 748, 1179, 1347
SsiI CCGC 8 cut(s) 47, 166, 588, 615, 630, 945, 1010, 1273
SspI AATATT 1 cut(s) 595
SspMI CTAG 4 cut(s) 132, 414, 672, 1247
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 9 cut(s) 230, 308, 370, 541, 609, 871, 898, 904, 910
StyI CCWWGG 3 cut(s) 131, 933, 1119
TaaI ACNGT 6 cut(s) 188, 424, 459, 579, 758, 799
TaiI ACGT 3 cut(s) 943, 1209, 1369
TaqI TCGA 4 cut(s) 622, 895, 1173, 1191
TasI AATT 3 cut(s) 748, 1179, 1347
TauI GCSGC 2 cut(s) 617, 1276
TfiI GAWTC 2 cut(s) 160, 1067
Tru1I TTAA 3 cut(s) 248, 381, 1077
Tru9I TTAA 3 cut(s) 248, 381, 1077
TscAI CASTG 4 cut(s) 31, 495, 804, 1173
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 3 cut(s) 107, 386, 1012
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 4 cut(s) 389, 1049, 1173, 1233
TspGWI ACGGA 3 cut(s) 47, 451, 669
TspRI CASTG 4 cut(s) 31, 495, 804, 1173
Tth111I GACNNNGTC 1 cut(s) 531
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 5 cut(s) 313, 533, 545, 626, 908
XagI CCTNNNNNAGG 2 cut(s) 540, 915
XceI RCATGY 1 cut(s) 776
XmaJI CCTAGG 1 cut(s) 131
XmiI GTMKAC 2 cut(s) 769, 895
XmnI GAANNNNTTC 2 cut(s) 849, 1056
XspI CTAG 4 cut(s) 132, 414, 672, 1247
Zsp2I ATGCAT 2 cut(s) 141, 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.