Rw0G023260

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01079
OG0007058
Physical Location & Seq
Reverse (-)
121161 .. 125737
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G023260.1

Sequence Viewer

Length: 1365 bp
ATGGCCTACGAGCTCTCGGATAGCAGTGGAACGGATGATGACCTTCCCCCATCTCATCAAAACCGCTTTCAAAGAGGAGGCCGAAGTTCTGGGAATGGAAGATCTGCAGCTGTTGGTTCTGCTCCATTACCTAGGATGCATGGTGATATGGAAACACAGATCCACCACATTGAGCAAGAAGCATACTGTTCAGTCCTGCGGGCATTTAAAGCCCAATCCGATGCCATCACCTGGGAGAAGGAAAGTTTAATAACGGAACTTAGAAAGGAGCTGAGAGTATCGGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCGAAGAATAAGGGAGTGGAGAAAAACCAGTGGGCCTCAGCCGGGTACACATAATGCCGCTCAGCCTGTTCATGACCCCATACCTAGTCCAACTGTTTCGGCATCACGTAAGAAACAGAAAACATCACAGTCTGTAGCTTCTTTATCACATGGTGGTGCTCCATCTCCAGTGCTGCCTCCATCCATGCAACCTTCCTCATCAGCCTTGAGACGTGGTCCTCCTCCAGGAGCTCGGAGCAAGAAGTCAAAATCATCCACACAGTTCCCTTCTACAAGTATTACCGGAAGGCCCCAAGCCCCAAACCGAGGTTCTTCAGGTGGCTTTGTGGCAACTGAGCCTGCTGAGGCAACAACTTATGATCCATTAATAGGAAGAAAAGTATGGACAAGGTGGCCTGCGGACAACCATTTCTATGAGGCTGTAATAACTGATTACAACCATATTCAGGGCCGACATGCTCTGGTTTATGATATCAATACAGCGGATGAAACATGGGAATGGGTCAACCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGGTGATGATCCTGGGTTTTCTCGTAGGGGTGGTCGACCTGGATCTGGCCGAGGGACTAAGAAGTCCATGGGACGTGGTGGTGCAGTCAGCCCTAAGAAGGATTTGCCTCCACAACAAAATGGCATTGGCAGGAAGGCCATGAGCGACATTGAAATACTTCATACAGACTCGTTGATTAAAGAGGTGGAAAAGGTCTTCAGTGCTAGCCATCCTGACCCGATGGAGATTGAGAAAGCTAAGAAAGTTCTCAAAGAGCATGAACAGGCATTGATCGATGCAATTGCAACGCTTGAAGATGCATCAGATGGTGAAAGTGATGAACATACATTCTCTCAAAGACAACCAATGGACCAAGAACGAGGATGGAGAAAACGGCAATATGATGAAATGGCAGAAGGTCAGGCAGTTGATGGTTCAAATGGTACTAAAATCATGAGAGATGGTAGAATTGCATCTGATGACCAACGTTTTGAAGGCGATGGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

454

Amino Acids

50.12

Weight (kDa)

6.34

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 52 - 120 3e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 937
AccB7I CCANNNNNTGG 1 cut(s) 231
AccBSI CCGCTC 1 cut(s) 392
AccI GTMKAC 1 cut(s) 910
AciI CCGC 5 cut(s) 64, 199, 390, 731, 815
AclI AACGTT 1 cut(s) 1342
AclWI GGATC 5 cut(s) 154, 325, 686, 878, 925
AcoI YGGCCR 1 cut(s) 922
AcuI CTGAAG 3 cut(s) 630, 879, 1057
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 2 cut(s) 379, 1300
AfiI CCNNNNNNNGG 8 cut(s) 231, 374, 557, 638, 701, 778, 920, 973
AgsI TTSAA 5 cut(s) 71, 1028, 1169, 1293, 1349
AjiI CACGTC 2 cut(s) 545, 950
AjnI CCWGG 5 cut(s) 230, 308, 556, 886, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
AluBI AGCT 6 cut(s) 13, 110, 271, 470, 563, 1112
AluI AGCT 6 cut(s) 13, 110, 271, 470, 563, 1112
Alw21I GWGCWC 3 cut(s) 15, 493, 565
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 5 cut(s) 154, 325, 686, 878, 925
AoxI GGCC 8 cut(s) 3, 79, 365, 620, 725, 781, 922, 1011
ApeKI GCWGC 2 cut(s) 107, 505
ArsI GACNNNNNNTTYG 2 cut(s) 1328, 1360
AseI ATTAAT 1 cut(s) 698
Asp700I GAANNNNTTC 2 cut(s) 864, 1032
AspA2I CCTAGG 1 cut(s) 131
AspS9I GGNCC 5 cut(s) 365, 548, 621, 781, 1225
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 4 cut(s) 155, 220, 890, 1196
AsuNHI GCTAGC 1 cut(s) 1079
AvaII GGWCC 2 cut(s) 548, 1225
AvrII CCTAGG 1 cut(s) 131
BanII GRGCYC 2 cut(s) 15, 565
BbsI GAAGAC 1 cut(s) 1063
Bbv12I GWGCWC 3 cut(s) 15, 493, 565
BbvCI CCTCAGC 2 cut(s) 369, 675
BbvI GCAGC 2 cut(s) 119, 492
BceAI ACGGC 1 cut(s) 1265
BcgI CGANNNNNNTGC 2 cut(s) 1139, 1173
BciT130I CCWGG 5 cut(s) 232, 310, 558, 888, 915
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 3 cut(s) 132, 417, 1080
BfmI CTRYAG 2 cut(s) 105, 465
BglII AGATCT 1 cut(s) 101
BisI GCNGC 3 cut(s) 108, 390, 506
BlnI CCTAGG 1 cut(s) 131
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 3 cut(s) 109, 391, 507
Bme1390I CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
Bme18I GGWCC 2 cut(s) 548, 1225
BmgBI CACGTC 2 cut(s) 545, 950
BmgT120I GGNCC 5 cut(s) 365, 548, 621, 781, 1225
BmiI GGNNCC 1 cut(s) 623
BmrFI CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
BmsI GCATC 7 cut(s) 126, 211, 443, 1141, 1162, 1184, 1337
BmtI GCTAGC 1 cut(s) 1083
BpiI GAAGAC 1 cut(s) 1063
BpmI CTGGAG 2 cut(s) 483, 540
Bpu10I CCTNAGC 2 cut(s) 369, 675
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 559
BpuMI CCSGG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 1149
BsaAI YACGTR 1 cut(s) 440
BsaJI CCNNGG 7 cut(s) 131, 231, 309, 637, 887, 925, 942
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 8 cut(s) 231, 374, 557, 638, 701, 778, 920, 973
Bse1I ACTGG 2 cut(s) 360, 500
BseBI CCWGG 5 cut(s) 232, 310, 558, 888, 915
BseCI ATCGAT 1 cut(s) 1149
BseDI CCNNGG 7 cut(s) 131, 231, 309, 637, 887, 925, 942
BseGI GGATG 8 cut(s) 40, 141, 289, 512, 584, 823, 1084, 1244
BseLI CCNNNNNNNGG 8 cut(s) 231, 374, 557, 638, 701, 778, 920, 973
BseMII CTCAG 5 cut(s) 263, 383, 407, 657, 666
BseNI ACTGG 2 cut(s) 360, 500
BseRI GAGGAG 2 cut(s) 90, 543
BseXI GCAGC 2 cut(s) 119, 492
BsgI GTGCAG 1 cut(s) 978
BshFI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1013
BshVI ATCGAT 1 cut(s) 1149
BsiHKAI GWGCWC 3 cut(s) 15, 493, 565
BsiSI CCGG 2 cut(s) 374, 615
BslFI GGGAC 2 cut(s) 943, 960
BslI CCNNNNNNNGG 8 cut(s) 231, 374, 557, 638, 701, 778, 920, 973
BsmAI GTCTC 1 cut(s) 535
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 943, 960
BsnI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1013
Bsp1286I GDGCHC 3 cut(s) 15, 493, 565
Bsp143I GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 5 cut(s) 64, 199, 390, 731, 815
BspANI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1013
BspCNI CTCAG 5 cut(s) 264, 382, 406, 658, 667
BspDI ATCGAT 1 cut(s) 1149
BspHI TCATGA 2 cut(s) 403, 1308
BspLI GGNNCC 1 cut(s) 623
BspMAI CTGCAG 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 1083
BspPI GGATC 5 cut(s) 154, 325, 686, 878, 925
BsrBI CCGCTC 1 cut(s) 392
BsrI ACTGG 2 cut(s) 360, 500
BssECI CCNNGG 7 cut(s) 131, 231, 309, 637, 887, 925, 942
BssMI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
BssT1I CCWWGG 2 cut(s) 131, 942
Bst2UI CCWGG 5 cut(s) 232, 310, 558, 888, 915
Bst4CI ACNGT 4 cut(s) 188, 427, 462, 594
BstBAI YACGTR 1 cut(s) 440
BstC8I GCNNGC 4 cut(s) 201, 672, 729, 1081
BstDEI CTNAG 9 cut(s) 260, 272, 369, 393, 666, 675, 933, 969, 1113
BstDSI CCRYGG 1 cut(s) 942
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 8 cut(s) 40, 141, 289, 512, 584, 823, 1084, 1244
BstKTI GATC 7 cut(s) 104, 162, 333, 694, 886, 920, 1149
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 5 cut(s) 232, 310, 558, 888, 915
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 6 cut(s) 230, 308, 373, 556, 886, 913
BstSFI CTRYAG 2 cut(s) 105, 465
BstV1I GCAGC 2 cut(s) 119, 492
BstV2I GAAGAC 1 cut(s) 1063
BstX2I RGATCY 3 cut(s) 101, 159, 917
BstYI RGATCY 3 cut(s) 101, 159, 917
Bsu15I ATCGAT 1 cut(s) 1149
BsuRI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1013
BsuTUI ATCGAT 1 cut(s) 1149
BtgI CCRYGG 1 cut(s) 942
BtrI CACGTC 2 cut(s) 545, 950
BtsCI GGATG 8 cut(s) 40, 141, 289, 512, 584, 823, 1084, 1244
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 4 cut(s) 31, 367, 507, 1081
Cac8I GCNNGC 4 cut(s) 201, 672, 729, 1081
CciI TCATGA 2 cut(s) 403, 1308
Cfr13I GGNCC 5 cut(s) 365, 548, 621, 781, 1225
ClaI ATCGAT 1 cut(s) 1149
Csp6I GTAC 2 cut(s) 378, 1299
CviQI GTAC 2 cut(s) 378, 1299
DdeI CTNAG 9 cut(s) 260, 272, 369, 393, 666, 675, 933, 969, 1113
DpnI GATC 7 cut(s) 103, 161, 332, 693, 885, 919, 1148
DpnII GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
DraI TTTAAA 1 cut(s) 208
DraIII CACNNNGTG 1 cut(s) 485
DrdI GACNNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 937
EaeI YGGCCR 1 cut(s) 922
Ecl136II GAGCTC 2 cut(s) 13, 563
Eco130I CCWWGG 2 cut(s) 131, 942
Eco24I GRGCYC 2 cut(s) 15, 565
Eco32I GATATC 2 cut(s) 805, 852
Eco47I GGWCC 2 cut(s) 548, 1225
Eco53kI GAGCTC 2 cut(s) 13, 563
Eco57I CTGAAG 3 cut(s) 630, 879, 1057
EcoICRI GAGCTC 2 cut(s) 13, 563
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 621
EcoRII CCWGG 5 cut(s) 230, 308, 556, 886, 913
EcoRV GATATC 2 cut(s) 805, 852
EcoT14I CCWWGG 2 cut(s) 131, 942
EcoT22I ATGCAT 2 cut(s) 141, 1177
EcoT38I GRGCYC 2 cut(s) 15, 565
ErhI CCWWGG 2 cut(s) 131, 942
Esp3I CGTCTC 1 cut(s) 535
FaqI GGGAC 2 cut(s) 943, 960
FauI CCCGC 1 cut(s) 192
FauNDI CATATG 1 cut(s) 1361
FblI GTMKAC 1 cut(s) 910
Fnu4HI GCNGC 3 cut(s) 108, 390, 506
FokI GGATG 8 cut(s) 47, 148, 296, 499, 571, 830, 1071, 1251
FriOI GRGCYC 2 cut(s) 15, 565
Fsp4HI GCNGC 3 cut(s) 108, 390, 506
FspBI CTAG 3 cut(s) 132, 417, 1080
GluI GCNGC 3 cut(s) 108, 390, 506
GsuI CTGGAG 2 cut(s) 483, 540
HaeIII GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1013
HapII CCGG 2 cut(s) 374, 615
HincII GTYRAC 2 cut(s) 838, 911
HindII GTYRAC 2 cut(s) 838, 911
HinfI GANTC 1 cut(s) 1043
HpaII CCGG 2 cut(s) 374, 615
HphI GGTGA 4 cut(s) 155, 220, 890, 1196
Hpy166II GTNNAC 3 cut(s) 380, 838, 911
Hpy188I TCNGA 7 cut(s) 19, 220, 283, 335, 567, 1180, 1333
Hpy188III TCNNGA 4 cut(s) 404, 857, 1088, 1309
Hpy8I GTNNAC 3 cut(s) 380, 838, 911
HpyCH4III ACNGT 4 cut(s) 188, 427, 462, 594
HpyCH4IV ACGT 4 cut(s) 439, 544, 949, 1342
HpyCH4V TGCA 8 cut(s) 107, 139, 520, 959, 1154, 1160, 1175, 1328
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 9 cut(s) 260, 272, 369, 393, 666, 675, 933, 969, 1113
HpySE526I ACGT 4 cut(s) 439, 544, 949, 1342
Kzo9I GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
LmnI GCTCC 5 cut(s) 127, 268, 496, 560, 567
Lsp1109I GCAGC 2 cut(s) 119, 492
LweI GCATC 7 cut(s) 126, 211, 443, 1141, 1162, 1184, 1337
MaeI CTAG 3 cut(s) 132, 417, 1080
MaeII ACGT 4 cut(s) 439, 544, 949, 1342
MalI GATC 7 cut(s) 103, 161, 332, 693, 885, 919, 1148
MbiI CCGCTC 1 cut(s) 392
MboI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
MboII GAAGA 7 cut(s) 111, 348, 636, 717, 872, 1063, 1181
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 3 cut(s) 101, 159, 917
MhlI GDGCHC 3 cut(s) 15, 493, 565
MluCI AATT 2 cut(s) 1155, 1323
MlyI GAGTC 1 cut(s) 1037
MmeI TCCRAC 1 cut(s) 446
Mph1103I ATGCAT 2 cut(s) 141, 1177
MroXI GAANNNNTTC 2 cut(s) 864, 1032
MseI TTAA 4 cut(s) 207, 248, 698, 1053
MslI CAYNNNNRTG 3 cut(s) 486, 744, 829
MspA1I CMGCKG 2 cut(s) 110, 815
MspI CCGG 2 cut(s) 374, 615
MspR9I CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
MunI CAATTG 1 cut(s) 1155
MvaI CCWGG 5 cut(s) 232, 310, 558, 888, 915
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 1 cut(s) 375
NcoI CCATGG 1 cut(s) 942
NdeI CATATG 1 cut(s) 1361
NdeII GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
NheI GCTAGC 1 cut(s) 1079
NlaIV GGNNCC 1 cut(s) 623
NmeAIII GCCGAG 1 cut(s) 950
NsiI ATGCAT 2 cut(s) 141, 1177
NspI RCATGY 1 cut(s) 791
PagI TCATGA 2 cut(s) 403, 1308
PdmI GAANNNNTTC 2 cut(s) 864, 1032
PflFI GACNNNGTC 1 cut(s) 546
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 3 cut(s) 109, 391, 507
PleI GAGTC 1 cut(s) 1037
PpsI GAGTC 1 cut(s) 1037
Ppu21I YACGTR 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 698
Psp124BI GAGCTC 2 cut(s) 15, 565
Psp1406I AACGTT 1 cut(s) 1342
Psp6I CCWGG 5 cut(s) 230, 308, 556, 886, 913
PspGI CCWGG 5 cut(s) 230, 308, 556, 886, 913
PspN4I GGNNCC 1 cut(s) 623
PspPI GGNCC 5 cut(s) 365, 548, 621, 781, 1225
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 3 cut(s) 101, 159, 917
PsyI GACNNNGTC 1 cut(s) 546
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 2 cut(s) 379, 1300
RsaNI GTAC 2 cut(s) 378, 1299
RseI CAYNNNNRTG 3 cut(s) 486, 744, 829
SacI GAGCTC 2 cut(s) 15, 565
SalI GTCGAC 1 cut(s) 909
SaqAI TTAA 4 cut(s) 207, 248, 698, 1053
SatI GCNGC 3 cut(s) 108, 390, 506
Sau3AI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1146
Sau96I GGNCC 5 cut(s) 365, 548, 621, 781, 1225
SchI GAGTC 1 cut(s) 1037
ScrFI CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
SduI GDGCHC 3 cut(s) 15, 493, 565
SfaNI GCATC 7 cut(s) 126, 211, 443, 1141, 1162, 1184, 1337
SfcI CTRYAG 2 cut(s) 105, 465
SinI GGWCC 2 cut(s) 548, 1225
SmiMI CAYNNNNRTG 3 cut(s) 486, 744, 829
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
Sse9I AATT 2 cut(s) 1155, 1323
SsiI CCGC 5 cut(s) 64, 199, 390, 731, 815
SspMI CTAG 3 cut(s) 132, 417, 1080
SstI GAGCTC 2 cut(s) 15, 565
StyD4I CCNGG 6 cut(s) 230, 308, 373, 556, 886, 913
StyI CCWWGG 2 cut(s) 131, 942
TaaI ACNGT 4 cut(s) 188, 427, 462, 594
TaiI ACGT 4 cut(s) 442, 547, 952, 1345
TaqI TCGA 2 cut(s) 910, 1149
TasI AATT 2 cut(s) 1155, 1323
TauI GCSGC 1 cut(s) 392
Tru1I TTAA 4 cut(s) 207, 248, 698, 1053
Tru9I TTAA 4 cut(s) 207, 248, 698, 1053
TscAI CASTG 4 cut(s) 31, 367, 507, 1081
TseI GCWGC 2 cut(s) 107, 505
TspDTI ATGAA 6 cut(s) 392, 834, 1025, 1149, 1209, 1275
TspGWI ACGGA 2 cut(s) 47, 269
TspRI CASTG 4 cut(s) 31, 367, 507, 1081
Tth111I GACNNNGTC 1 cut(s) 546
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 2 cut(s) 548, 1225
VspI ATTAAT 1 cut(s) 698
XagI CCTNNNNNAGG 1 cut(s) 555
XceI RCATGY 1 cut(s) 791
XmaJI CCTAGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 910
XmnI GAANNNNTTC 2 cut(s) 864, 1032
XspI CTAG 3 cut(s) 132, 417, 1080
Zsp2I ATGCAT 2 cut(s) 141, 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.