MD03G1018600.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007058
Physical Location & Seq
Reverse (-)
1443682 .. 1448858
5177 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1018600.v1.1.491

Sequence Viewer

Length: 1269 bp
ATGGACTACGAACTCTCAGATAGCAGTGGAACGGATGATGACCTTCCGCCGTCTCATCAAAATAGGTTTCAAAGAGGAGGCCGAACTTCTGGGAATGGAAGGTCAGCAGCCGTCGGTTCTGCTCCATTATCTAGGATGCATGGTGACATGGAAATCCAGATTCACCGCATTGAGCAAGAAGCATACTGTTCAGTTCTAAGGGCGTTTAGTGCTCAATCTGATGCCCTCACCTGGGATAAGGAAACTTTAATTACGGAACTTAGAAAGGAGCTGAGAGTATCTGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCAGAGATTAAGGGAATGGAGAAAAACAAGTGGGGTCCAACATGGTACACTTAATGCTGCTCAGCCTGTTCATGACCCCCTGCCTAGTCCAACTGTTTCAGCATCACGCAAGAAACAGAAAACATCACAGTCCTTATCCCTCGGTGCTGCAACTCCTGCGTTGCCTCCATCCATGCAACAATCCTCTGCAGCTTTGAGACGAGGTCCTCCTCCAGGACCTCGGAACAAGAAACCAAAATCTTCCACACAGTACCCTTCTGCAAATATTCCTGGAAGGCCCCAAGCCACTAATCGAGGTCCTACCGGTGCCTTTGTGGAAAATGAGCCTGCTGAGGGAGAAACTGATGATCCGTTAGTAGGAAGAAAAGTTTGGACAAGGTGGCCTGAAGACAATCACTTCTATGAGGCTGTTATAACTAATTTCAACCGTGCTGAGGGTCTACATGCTCTGGTTTATGATATTGGTACAGTGGATGAGACATGGGAATGGGTCAATCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGCTGATGATCCTGGGATTTCTCGTAAAGGCGGTCGACCTGGACCTGGACCTGGACCTGGCCGAGGGAATAAAAAGTCCACGGCACGTGGTGGTGCAGTTGCAGGTGCAGGAAGAGGTAGAGGGACTGCAAAGGGTTCCAAGAAGGATTTGCCTCCGCAACTAAATGGCATTGGCAGGAAGGCAATGAGCGACATTGAAATACTTCATACAGGCTCGTTGATTAAAGAGGTGGAAAAGGTTTTCAGAGCCAGCCATCCTGACCCCATGGAGATTGAGAAAGCCAAGAAAGTTCTCAAAGAGCATGAACAGGCACTGGTCGATGCAATTGCAAGACTCGAAGATGCATCTGACGTTGAAAGCGTGAATGTATCTCTGAAGCAGCACGGCTGCGCAACGTCTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

423

Amino Acids

46.06

Weight (kDa)

7.23

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 53 - 120 2.1e-30 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 746
AarI CACCTGC 1 cut(s) 956
Acc16I TGCGCA 1 cut(s) 1255
Acc36I ACCTGC 1 cut(s) 956
AccB1I GGYRCC 1 cut(s) 638
AccI GTMKAC 2 cut(s) 772, 898
AciI CCGC 4 cut(s) 47, 166, 894, 1019
AclWI GGATC 3 cut(s) 325, 674, 866
AcoI YGGCCR 1 cut(s) 922
AcuI CTGAAG 3 cut(s) 738, 867, 1259
AcvI CACGTG 1 cut(s) 950
AdeI CACNNNGTG 1 cut(s) 953
AfaI GTAC 3 cut(s) 379, 584, 799
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 4 cut(s) 71, 757, 1061, 1220
AjnI CCWGG 9 cut(s) 230, 308, 544, 601, 874, 901, 907, 913, 919
AjuI GAANNNNNNNTTGG 2 cut(s) 685, 717
AluBI AGCT 3 cut(s) 271, 524, 866
AluI AGCT 3 cut(s) 271, 524, 866
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 3 cut(s) 57, 523, 803
AlwI GGATC 3 cut(s) 325, 674, 866
AlwNI CAGNNNCTG 1 cut(s) 1177
AoxI GGCC 4 cut(s) 79, 608, 713, 922
ApeKI GCWGC 6 cut(s) 107, 389, 479, 521, 1243, 1251
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 2 cut(s) 852, 1065
AspLEI GCGC 1 cut(s) 1256
AspS9I GGNCC 8 cut(s) 367, 536, 548, 609, 629, 905, 911, 917
AsuHPI GGTGA 3 cut(s) 155, 155, 220
AvaII GGWCC 7 cut(s) 367, 536, 548, 629, 905, 911, 917
BanI GGYRCC 1 cut(s) 638
BbrPI CACGTG 1 cut(s) 950
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 214
BbvCI CCTCAGC 2 cut(s) 663, 765
BbvI GCAGC 6 cut(s) 119, 376, 466, 533, 1238, 1255
BccI CCATC 2 cut(s) 508, 1125
BceAI ACGGC 4 cut(s) 34, 95, 960, 1264
BcgI CGANNNNNNTGC 2 cut(s) 1172, 1206
BciT130I CCWGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
BcoDI GTCTC 3 cut(s) 57, 523, 803
BfaI CTAG 2 cut(s) 132, 417
BfmI CTRYAG 1 cut(s) 519
BfuAI ACCTGC 1 cut(s) 956
BisI GCNGC 6 cut(s) 108, 390, 480, 522, 1244, 1252
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 6 cut(s) 109, 391, 481, 523, 1245, 1253
Bme1390I CCNGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
Bme18I GGWCC 7 cut(s) 367, 536, 548, 629, 905, 911, 917
BmgT120I GGNCC 8 cut(s) 367, 536, 548, 609, 629, 905, 911, 917
BmiI GGNNCC 4 cut(s) 368, 611, 640, 1000
BmrFI CCNGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
BmsI GCATC 6 cut(s) 126, 211, 443, 1174, 1195, 1217
BpiI GAAGAC 1 cut(s) 726
BpmI CTGGAG 1 cut(s) 528
Bpu10I CCTNAGC 2 cut(s) 663, 765
Bpu1102I GCTNAGC 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 950
BsaJI CCNNGG 8 cut(s) 231, 309, 472, 551, 875, 925, 942, 1128
BsaWI WCCGGW 1 cut(s) 635
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 1 cut(s) 1182
Bse3DI GCAATG 1 cut(s) 1053
BseBI CCWGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
BseDI CCNNGG 8 cut(s) 231, 309, 472, 551, 875, 925, 942, 1128
BseGI GGATG 5 cut(s) 40, 141, 500, 811, 1117
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 5 cut(s) 30, 263, 407, 654, 756
BseNI ACTGG 1 cut(s) 1182
BseRI GAGGAG 2 cut(s) 90, 531
BseXI GCAGC 6 cut(s) 119, 376, 466, 533, 1238, 1255
BsgI GTGCAG 2 cut(s) 978, 990
Bsh1285I CGRYCG 1 cut(s) 898
BshFI GGCC 4 cut(s) 81, 610, 715, 924
BshNI GGYRCC 1 cut(s) 638
BshTI ACCGGT 1 cut(s) 635
BsiEI CGRYCG 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 214
BsiSI CCGG 1 cut(s) 636
BslFI GGGAC 1 cut(s) 1000
BsmAI GTCTC 3 cut(s) 57, 523, 803
BsmBI CGTCTC 2 cut(s) 57, 523
BsmFI GGGAC 1 cut(s) 1000
BsnI GGCC 4 cut(s) 81, 610, 715, 924
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 3 cut(s) 330, 679, 871
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 1128
BspACI CCGC 4 cut(s) 47, 166, 894, 1019
BspANI GGCC 4 cut(s) 81, 610, 715, 924
BspCNI CTCAG 5 cut(s) 29, 264, 406, 655, 757
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 4 cut(s) 368, 611, 640, 1000
BspMAI CTGCAG 1 cut(s) 523
BspMI ACCTGC 1 cut(s) 956
BspPI GGATC 3 cut(s) 325, 674, 866
BspT107I GGYRCC 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 1053
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 1 cut(s) 1182
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 8 cut(s) 231, 309, 472, 551, 875, 925, 942, 1128
BssMI GATC 3 cut(s) 330, 679, 871
BssT1I CCWWGG 1 cut(s) 1128
Bst2UI CCWGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
Bst4CI ACNGT 6 cut(s) 188, 427, 462, 582, 761, 802
Bst6I CTCTTC 1 cut(s) 970
BstAPI GCANNNNNTGC 1 cut(s) 488
BstBAI YACGTR 1 cut(s) 950
BstC8I GCNNGC 2 cut(s) 660, 1114
BstDEI CTNAG 7 cut(s) 16, 197, 260, 272, 393, 663, 765
BstDSI CCRYGG 2 cut(s) 942, 1128
BstENI CCTNNNNNAGG 2 cut(s) 543, 924
BstF5I GGATG 5 cut(s) 40, 141, 500, 811, 1117
BstHHI GCGC 1 cut(s) 1256
BstKTI GATC 3 cut(s) 333, 682, 874
BstMAI GTCTC 3 cut(s) 57, 523, 803
BstMBI GATC 3 cut(s) 330, 679, 871
BstMCI CGRYCG 1 cut(s) 898
BstMWI GCNNNNNNNGC 2 cut(s) 209, 488
BstNI CCWGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
BstNSI RCATGY 1 cut(s) 779
BstSCI CCNGG 9 cut(s) 230, 308, 544, 601, 874, 901, 907, 913, 919
BstSFI CTRYAG 1 cut(s) 519
BstV1I GCAGC 6 cut(s) 119, 376, 466, 533, 1238, 1255
BstV2I GAAGAC 1 cut(s) 726
BsuRI GGCC 4 cut(s) 81, 610, 715, 924
BtgI CCRYGG 2 cut(s) 942, 1128
BtsCI GGATG 5 cut(s) 40, 141, 500, 811, 1117
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 3 cut(s) 31, 807, 1175
BveI ACCTGC 1 cut(s) 956
Cac8I GCNNGC 2 cut(s) 660, 1114
CaiI CAGNNNCTG 1 cut(s) 1177
CciI TCATGA 1 cut(s) 403
CfoI GCGC 1 cut(s) 1256
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 8 cut(s) 367, 536, 548, 609, 629, 905, 911, 917
Csp6I GTAC 3 cut(s) 378, 583, 798
CspAI ACCGGT 1 cut(s) 635
CviQI GTAC 3 cut(s) 378, 583, 798
DdeI CTNAG 7 cut(s) 16, 197, 260, 272, 393, 663, 765
DpnI GATC 3 cut(s) 332, 681, 873
DpnII GATC 3 cut(s) 330, 679, 871
DraIII CACNNNGTG 1 cut(s) 953
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 1 cut(s) 970
EarI CTCTTC 1 cut(s) 970
EciI GGCGGA 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 1128
Eco32I GATATC 1 cut(s) 840
Eco47I GGWCC 7 cut(s) 367, 536, 548, 629, 905, 911, 917
Eco57I CTGAAG 3 cut(s) 738, 867, 1259
Eco72I CACGTG 1 cut(s) 950
EcoNI CCTNNNNNAGG 2 cut(s) 543, 924
EcoO109I RGGNCCY 4 cut(s) 536, 548, 609, 629
EcoRII CCWGG 9 cut(s) 230, 308, 544, 601, 874, 901, 907, 913, 919
EcoRV GATATC 1 cut(s) 840
EcoT14I CCWWGG 1 cut(s) 1128
EcoT22I ATGCAT 2 cut(s) 141, 1210
ErhI CCWWGG 1 cut(s) 1128
Esp3I CGTCTC 2 cut(s) 57, 523
FaqI GGGAC 1 cut(s) 1000
FblI GTMKAC 2 cut(s) 772, 898
Fnu4HI GCNGC 6 cut(s) 108, 390, 480, 522, 1244, 1252
FokI GGATG 5 cut(s) 47, 148, 487, 818, 1104
Fsp4HI GCNGC 6 cut(s) 108, 390, 480, 522, 1244, 1252
FspBI CTAG 2 cut(s) 132, 417
FspI TGCGCA 1 cut(s) 1255
GlaI GCGC 1 cut(s) 1255
GluI GCNGC 6 cut(s) 108, 390, 480, 522, 1244, 1252
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 4 cut(s) 81, 610, 715, 924
HapII CCGG 1 cut(s) 636
HhaI GCGC 1 cut(s) 1256
Hin6I GCGC 1 cut(s) 1254
HinP1I GCGC 1 cut(s) 1254
HincII GTYRAC 1 cut(s) 899
HindII GTYRAC 1 cut(s) 899
HinfI GANTC 2 cut(s) 160, 1197
HpaII CCGG 1 cut(s) 636
HphI GGTGA 3 cut(s) 155, 155, 220
Hpy166II GTNNAC 4 cut(s) 380, 773, 899, 942
Hpy188I TCNGA 7 cut(s) 19, 220, 283, 555, 1109, 1213, 1239
Hpy188III TCNNGA 5 cut(s) 157, 334, 404, 845, 1121
Hpy8I GTNNAC 4 cut(s) 380, 773, 899, 942
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 6 cut(s) 53, 93, 597, 600, 1000, 1036
HpyCH4III ACNGT 6 cut(s) 188, 427, 462, 582, 761, 802
HpyCH4IV ACGT 3 cut(s) 949, 1215, 1259
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 488
HpyF3I CTNAG 7 cut(s) 16, 197, 260, 272, 393, 663, 765
HpySE526I ACGT 3 cut(s) 949, 1215, 1259
HspAI GCGC 1 cut(s) 1254
Kzo9I GATC 3 cut(s) 330, 679, 871
LmnI GCTCC 2 cut(s) 127, 268
Lsp1109I GCAGC 6 cut(s) 119, 376, 466, 533, 1238, 1255
LweI GCATC 6 cut(s) 126, 211, 443, 1174, 1195, 1217
MaeI CTAG 2 cut(s) 132, 417
MaeII ACGT 3 cut(s) 949, 1215, 1259
MaeIII GTNAC 1 cut(s) 143
MalI GATC 3 cut(s) 332, 681, 873
MboI GATC 3 cut(s) 330, 679, 871
MboII GAAGA 6 cut(s) 564, 705, 731, 860, 987, 1214
MfeI CAATTG 1 cut(s) 1188
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 3 cut(s) 249, 751, 1188
MlyI GAGTC 1 cut(s) 1191
MmeI TCCRAC 2 cut(s) 394, 446
Mph1103I ATGCAT 2 cut(s) 141, 1210
MroXI GAANNNNTTC 2 cut(s) 852, 1065
MseI TTAA 5 cut(s) 248, 341, 384, 1086, 1267
MslI CAYNNNNRTG 2 cut(s) 732, 817
MspI CCGG 1 cut(s) 636
MspR9I CCNGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
MunI CAATTG 1 cut(s) 1188
MvaI CCWGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
MwoI GCNNNNNNNGC 2 cut(s) 209, 488
NcoI CCATGG 1 cut(s) 1128
NdeII GATC 3 cut(s) 330, 679, 871
NlaIV GGNNCC 4 cut(s) 368, 611, 640, 1000
NmeAIII GCCGAG 1 cut(s) 950
NmuCI GTSAC 1 cut(s) 143
NsbI TGCGCA 1 cut(s) 1255
NsiI ATGCAT 2 cut(s) 141, 1210
NspI RCATGY 1 cut(s) 779
PagI TCATGA 1 cut(s) 403
PaqCI CACCTGC 1 cut(s) 956
PcsI WCGNNNNNNNCGW 1 cut(s) 1221
PdmI GAANNNNTTC 2 cut(s) 852, 1065
PfeI GAWTC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 534
PfoI TCCNGGA 2 cut(s) 544, 601
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 6 cut(s) 109, 391, 481, 523, 1245, 1253
PleI GAGTC 1 cut(s) 1191
PmaCI CACGTG 1 cut(s) 950
PmlI CACGTG 1 cut(s) 950
PpsI GAGTC 1 cut(s) 1191
Ppu21I YACGTR 1 cut(s) 950
PpuMI RGGWCCY 3 cut(s) 536, 548, 629
PsiI TTATAA 1 cut(s) 746
Psp5II RGGWCCY 3 cut(s) 536, 548, 629
Psp6I CCWGG 9 cut(s) 230, 308, 544, 601, 874, 901, 907, 913, 919
PspCI CACGTG 1 cut(s) 950
PspGI CCWGG 9 cut(s) 230, 308, 544, 601, 874, 901, 907, 913, 919
PspN4I GGNNCC 4 cut(s) 368, 611, 640, 1000
PspPI GGNCC 8 cut(s) 367, 536, 548, 609, 629, 905, 911, 917
PspPPI RGGWCCY 3 cut(s) 536, 548, 629
PstI CTGCAG 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 1177
PsyI GACNNNGTC 1 cut(s) 534
RsaI GTAC 3 cut(s) 379, 584, 799
RsaNI GTAC 3 cut(s) 378, 583, 798
RseI CAYNNNNRTG 2 cut(s) 732, 817
SalI GTCGAC 1 cut(s) 897
SaqAI TTAA 5 cut(s) 248, 341, 384, 1086, 1267
SatI GCNGC 6 cut(s) 108, 390, 480, 522, 1244, 1252
Sau3AI GATC 3 cut(s) 330, 679, 871
Sau96I GGNCC 8 cut(s) 367, 536, 548, 609, 629, 905, 911, 917
SchI GAGTC 1 cut(s) 1191
ScrFI CCNGG 9 cut(s) 232, 310, 546, 603, 876, 903, 909, 915, 921
SduI GDGCHC 1 cut(s) 214
SfaNI GCATC 6 cut(s) 126, 211, 443, 1174, 1195, 1217
SfcI CTRYAG 1 cut(s) 519
SinI GGWCC 7 cut(s) 367, 536, 548, 629, 905, 911, 917
SmiMI CAYNNNNRTG 2 cut(s) 732, 817
Sse9I AATT 3 cut(s) 249, 751, 1188
SsiI CCGC 4 cut(s) 47, 166, 894, 1019
SspI AATATT 1 cut(s) 598
SspMI CTAG 2 cut(s) 132, 417
StyD4I CCNGG 9 cut(s) 230, 308, 544, 601, 874, 901, 907, 913, 919
StyI CCWWGG 1 cut(s) 1128
TaaI ACNGT 6 cut(s) 188, 427, 462, 582, 761, 802
TaiI ACGT 3 cut(s) 952, 1218, 1262
TaqI TCGA 4 cut(s) 625, 898, 1182, 1200
TasI AATT 3 cut(s) 249, 751, 1188
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 5 cut(s) 248, 341, 384, 1086, 1267
Tru9I TTAA 5 cut(s) 248, 341, 384, 1086, 1267
TscAI CASTG 3 cut(s) 31, 807, 1182
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 6 cut(s) 107, 389, 479, 521, 1243, 1251
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 392, 1058, 1182
TspGWI ACGGA 3 cut(s) 47, 269, 672
TspRI CASTG 3 cut(s) 31, 807, 1182
Tth111I GACNNNGTC 1 cut(s) 534
VpaK11BI GGWCC 7 cut(s) 367, 536, 548, 629, 905, 911, 917
XagI CCTNNNNNAGG 2 cut(s) 543, 924
XceI RCATGY 1 cut(s) 779
XmiI GTMKAC 2 cut(s) 772, 898
XmnI GAANNNNTTC 2 cut(s) 852, 1065
XspI CTAG 2 cut(s) 132, 417
Zsp2I ATGCAT 2 cut(s) 141, 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.