MD11G1020100.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007058
Physical Location & Seq
Reverse (-)
1730970 .. 1738105
7136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1020100.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGACTACGAGCTCTCTGATAGCAGTGGAACGGACGATGACTTTCTGCCGTCTCATCAAAATAGGTTTCAAAGAGGAGGCCGAACTTCTGGGAATGGAAGATCTGCAGCCGTCAGTTCTGCTCCTTTACCTAGGATGCATGGTGACATGGAAACCCAGATTCACCGCATTGAGCAAGAAGCATACTGTTCACTTCTAAGAGCATTTAGTGCTCAATCTGATGCCATCACCTGGGATAAGGAAACTTTAATGACAAATCTTAGAAAGGAGCTGAGAGTATCTGATGAGGAGCACAGAGAACTTTTGTCCAGGGTCCAGGCCGATGACATGATCCAGAGAATAAGGCAATGGAGAAAAAGTGGGCTTCAACCTGGTACACTTAATGCTGCTCAGCCTGTTCATGACCCCCTACCTAGTCCAACTGTTTCAGCATCACGTAAGAAACAGAAAACATCACAGTCCTTATCCCTCGGTGCTCCAACTCCTGAACTGCCTCAATCCATGCAACCATCCTCTTCAGCCTTGAGACGAGGTCCTCCTCCAGGACCTCGGAACAAGAAACCAAAATCTCCTGCTGGGGGAGAAACTAGTGATCCGTTAGTAGGAAGAAAGGTTTGGACAAGGTGGCCTGAAGACAACCACTTCTATGAGGCTGTTATAACTAATTTCAACCGTGCTGAGAACTCTCTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

232

Amino Acids

25.77

Weight (kDa)

8.95

Isoelectric Point (pI)

56.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 52 - 120 1.8e-30 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 659
AccB7I CCANNNNNTGG 1 cut(s) 231
AciI CCGC 1 cut(s) 166
AclWI GGATC 2 cut(s) 325, 587
AcuI CTGAAG 2 cut(s) 501, 651
AfaI GTAC 1 cut(s) 376
AfiI CCNNNNNNNGG 4 cut(s) 231, 542, 578, 602
AgsI TTSAA 3 cut(s) 71, 368, 670
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 5 cut(s) 230, 308, 315, 370, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 598, 630
AluBI AGCT 2 cut(s) 13, 271
AluI AGCT 2 cut(s) 13, 271
Alw21I GWGCWC 4 cut(s) 15, 214, 294, 478
Alw26I GTCTC 2 cut(s) 57, 520
AlwI GGATC 2 cut(s) 325, 587
AoxI GGCC 3 cut(s) 79, 318, 626
ApeKI GCWGC 2 cut(s) 107, 386
AspA2I CCTAGG 1 cut(s) 131
AspS9I GGNCC 3 cut(s) 313, 533, 545
AsuHPI GGTGA 3 cut(s) 155, 155, 220
AvaII GGWCC 3 cut(s) 313, 533, 545
AvrII CCTAGG 1 cut(s) 131
BanII GRGCYC 1 cut(s) 15
BbsI GAAGAC 1 cut(s) 639
Bbv12I GWGCWC 4 cut(s) 15, 214, 294, 478
BbvI GCAGC 2 cut(s) 119, 373
BccI CCATC 2 cut(s) 233, 517
BceAI ACGGC 2 cut(s) 34, 95
BciT130I CCWGG 5 cut(s) 232, 310, 317, 372, 543
BcoDI GTCTC 2 cut(s) 57, 520
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 3 cut(s) 132, 414, 588
BfmI CTRYAG 1 cut(s) 105
BglII AGATCT 1 cut(s) 101
BisI GCNGC 2 cut(s) 108, 387
BlnI CCTAGG 1 cut(s) 131
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 2 cut(s) 109, 388
Bme1390I CCNGG 5 cut(s) 232, 310, 317, 372, 543
Bme18I GGWCC 3 cut(s) 313, 533, 545
BmgT120I GGNCC 3 cut(s) 313, 533, 545
BmiI GGNNCC 1 cut(s) 314
BmrFI CCNGG 5 cut(s) 232, 310, 317, 372, 543
BmsI GCATC 3 cut(s) 126, 211, 440
BpiI GAAGAC 1 cut(s) 639
BpmI CTGGAG 1 cut(s) 525
Bpu1102I GCTNAGC 1 cut(s) 390
BpuEI CTTGAG 1 cut(s) 544
BsaAI YACGTR 1 cut(s) 437
BsaJI CCNNGG 5 cut(s) 131, 231, 309, 469, 548
BsaXI ACNNNNNCTCC 2 cut(s) 343, 373
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 542, 578, 602
Bse3DI GCAATG 1 cut(s) 353
BseBI CCWGG 5 cut(s) 232, 310, 317, 372, 543
BseDI CCNNGG 5 cut(s) 131, 231, 309, 469, 548
BseGI GGATG 2 cut(s) 141, 509
BseLI CCNNNNNNNGG 4 cut(s) 231, 542, 578, 602
BseMI GCAATG 1 cut(s) 353
BseMII CTCAG 3 cut(s) 263, 404, 669
BseRI GAGGAG 3 cut(s) 90, 302, 528
BseXI GCAGC 2 cut(s) 119, 373
BseYI CCCAGC 1 cut(s) 575
BshFI GGCC 3 cut(s) 81, 320, 628
BsiHKAI GWGCWC 4 cut(s) 15, 214, 294, 478
BslI CCNNNNNNNGG 4 cut(s) 231, 542, 578, 602
BsmAI GTCTC 2 cut(s) 57, 520
BsmBI CGTCTC 2 cut(s) 57, 520
BsnI GGCC 3 cut(s) 81, 320, 628
Bsp1286I GDGCHC 4 cut(s) 15, 214, 294, 478
Bsp143I GATC 3 cut(s) 101, 330, 592
Bsp1720I GCTNAGC 1 cut(s) 390
BspACI CCGC 1 cut(s) 166
BspANI GGCC 3 cut(s) 81, 320, 628
BspCNI CTCAG 3 cut(s) 264, 403, 670
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 1 cut(s) 314
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 2 cut(s) 325, 587
BsrDI GCAATG 1 cut(s) 353
BssECI CCNNGG 5 cut(s) 131, 231, 309, 469, 548
BssMI GATC 3 cut(s) 101, 330, 592
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 5 cut(s) 232, 310, 317, 372, 543
Bst4CI ACNGT 4 cut(s) 188, 424, 459, 674
Bst6I CTCTTC 1 cut(s) 520
BstAPI GCANNNNNTGC 1 cut(s) 209
BstBAI YACGTR 1 cut(s) 437
BstDEI CTNAG 5 cut(s) 197, 260, 272, 390, 678
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 2 cut(s) 141, 509
BstKTI GATC 3 cut(s) 104, 333, 595
BstMAI GTCTC 2 cut(s) 57, 520
BstMBI GATC 3 cut(s) 101, 330, 592
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 5 cut(s) 232, 310, 317, 372, 543
BstSCI CCNGG 5 cut(s) 230, 308, 315, 370, 541
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 119, 373
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 3 cut(s) 81, 320, 628
BtsCI GGATG 2 cut(s) 141, 509
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 31
CciI TCATGA 1 cut(s) 400
Cfr13I GGNCC 3 cut(s) 313, 533, 545
CsiI ACCWGGT 1 cut(s) 370
Csp6I GTAC 1 cut(s) 375
CviAII CATG 5 cut(s) 140, 148, 328, 401, 502
CviQI GTAC 1 cut(s) 375
DdeI CTNAG 5 cut(s) 197, 260, 272, 390, 678
DpnI GATC 3 cut(s) 103, 332, 594
DpnII GATC 3 cut(s) 101, 330, 592
Eam1104I CTCTTC 1 cut(s) 520
EarI CTCTTC 1 cut(s) 520
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 131
Eco24I GRGCYC 1 cut(s) 15
Eco47I GGWCC 3 cut(s) 313, 533, 545
Eco53kI GAGCTC 1 cut(s) 13
Eco57I CTGAAG 2 cut(s) 501, 651
EcoICRI GAGCTC 1 cut(s) 13
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO109I RGGNCCY 2 cut(s) 533, 545
EcoRII CCWGG 5 cut(s) 230, 308, 315, 370, 541
EcoT14I CCWWGG 1 cut(s) 131
EcoT22I ATGCAT 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 131
Esp3I CGTCTC 2 cut(s) 57, 520
FaeI CATG 5 cut(s) 143, 151, 331, 404, 505
FaiI YATR 8 cut(s) 141, 149, 184, 329, 402, 503, 648, 659
FatI CATG 5 cut(s) 139, 147, 327, 400, 501
Fnu4HI GCNGC 2 cut(s) 108, 387
FokI GGATG 2 cut(s) 148, 496
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 2 cut(s) 108, 387
FspBI CTAG 3 cut(s) 132, 414, 588
GluI GCNGC 2 cut(s) 108, 387
GsaI CCCAGC 1 cut(s) 579
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 3 cut(s) 81, 320, 628
Hin1II CATG 5 cut(s) 143, 151, 331, 404, 505
HinfI GANTC 1 cut(s) 160
HphI GGTGA 3 cut(s) 155, 155, 220
Hpy166II GTNNAC 2 cut(s) 191, 377
Hpy188I TCNGA 4 cut(s) 19, 220, 283, 552
Hpy188III TCNNGA 3 cut(s) 334, 401, 485
Hpy8I GTNNAC 2 cut(s) 191, 377
HpyCH4III ACNGT 4 cut(s) 188, 424, 459, 674
HpyCH4IV ACGT 1 cut(s) 436
HpyCH4V TGCA 3 cut(s) 107, 139, 505
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 5 cut(s) 197, 260, 272, 390, 678
HpySE526I ACGT 1 cut(s) 436
Hsp92II CATG 5 cut(s) 143, 151, 331, 404, 505
Kzo9I GATC 3 cut(s) 101, 330, 592
LmnI GCTCC 4 cut(s) 127, 268, 289, 481
Lsp1109I GCAGC 2 cut(s) 119, 373
LweI GCATC 3 cut(s) 126, 211, 440
MabI ACCWGGT 1 cut(s) 370
MaeI CTAG 3 cut(s) 132, 414, 588
MaeII ACGT 1 cut(s) 436
MaeIII GTNAC 1 cut(s) 143
MalI GATC 3 cut(s) 103, 332, 594
MboI GATC 3 cut(s) 101, 330, 592
MboII GAAGA 4 cut(s) 111, 507, 618, 644
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 4 cut(s) 15, 214, 294, 478
MluCI AATT 1 cut(s) 664
MmeI TCCRAC 2 cut(s) 443, 503
Mph1103I ATGCAT 1 cut(s) 141
MseI TTAA 2 cut(s) 248, 381
MslI CAYNNNNRTG 1 cut(s) 645
MspR9I CCNGG 5 cut(s) 232, 310, 317, 372, 543
MvaI CCWGG 5 cut(s) 232, 310, 317, 372, 543
MwoI GCNNNNNNNGC 1 cut(s) 209
NdeII GATC 3 cut(s) 101, 330, 592
NlaIII CATG 5 cut(s) 143, 151, 331, 404, 505
NlaIV GGNNCC 1 cut(s) 314
NmuCI GTSAC 1 cut(s) 143
NsiI ATGCAT 1 cut(s) 141
PagI TCATGA 1 cut(s) 400
PfeI GAWTC 1 cut(s) 160
PflFI GACNNNGTC 1 cut(s) 531
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 541
PkrI GCNGC 2 cut(s) 109, 388
Ppu21I YACGTR 1 cut(s) 437
PpuMI RGGWCCY 2 cut(s) 533, 545
PsiI TTATAA 1 cut(s) 659
Psp124BI GAGCTC 1 cut(s) 15
Psp5II RGGWCCY 2 cut(s) 533, 545
Psp6I CCWGG 5 cut(s) 230, 308, 315, 370, 541
PspFI CCCAGC 1 cut(s) 575
PspGI CCWGG 5 cut(s) 230, 308, 315, 370, 541
PspN4I GGNNCC 1 cut(s) 314
PspPI GGNCC 3 cut(s) 313, 533, 545
PspPPI RGGWCCY 2 cut(s) 533, 545
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 101
PsyI GACNNNGTC 1 cut(s) 531
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 1 cut(s) 645
SacI GAGCTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 248, 381
SatI GCNGC 2 cut(s) 108, 387
Sau3AI GATC 3 cut(s) 101, 330, 592
Sau96I GGNCC 3 cut(s) 313, 533, 545
ScrFI CCNGG 5 cut(s) 232, 310, 317, 372, 543
SduI GDGCHC 4 cut(s) 15, 214, 294, 478
SexAI ACCWGGT 1 cut(s) 370
SfaNI GCATC 3 cut(s) 126, 211, 440
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 3 cut(s) 313, 533, 545
SmiMI CAYNNNNRTG 1 cut(s) 645
SmlI CTYRAG 1 cut(s) 523
SmoI CTYRAG 1 cut(s) 523
SpeI ACTAGT 1 cut(s) 587
Sse9I AATT 1 cut(s) 664
SsiI CCGC 1 cut(s) 166
SspMI CTAG 3 cut(s) 132, 414, 588
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 5 cut(s) 230, 308, 315, 370, 541
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 4 cut(s) 188, 424, 459, 674
TaiI ACGT 1 cut(s) 439
TasI AATT 1 cut(s) 664
TfiI GAWTC 1 cut(s) 160
Tru1I TTAA 2 cut(s) 248, 381
Tru9I TTAA 2 cut(s) 248, 381
TscAI CASTG 1 cut(s) 31
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 2 cut(s) 107, 386
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 1 cut(s) 389
TspGWI ACGGA 2 cut(s) 47, 585
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 531
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 3 cut(s) 313, 533, 545
XagI CCTNNNNNAGG 1 cut(s) 540
XmaJI CCTAGG 1 cut(s) 131
XspI CTAG 3 cut(s) 132, 414, 588
Zsp2I ATGCAT 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.