Rh5AG522500

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007058
Physical Location & Seq
Forward (+)
89642797 .. 89647989
5193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG522500.1

Sequence Viewer

Length: 1239 bp
ATGGCCTACGAGCTCTCGGATAGCAGTGGAACGGATGATGACCTTCCCCCATCTCATCAAAACCGCTTTCAAAGAGGAGGCCGAAGTTCTGGGAATGGAAGATCTGCAGCTGTTGGTTCTGCTCCATTACCTAGGATGCATGGTGATATGGAAACACAGATCCACCACATTGAGCAAGAAGCATACTGTTCAGTCCTGCGGGCATTTAAAGCCCAATCTGATGCCATCACCTGGGAGAAGGAAAGTTTAATAACGGAACTTAGAAAGGAGCTGAGAGTATCGGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCGAAGAATAAGGGAGTGGAGAAAAACCAGTGGGCCTCAACCGGGTACACATAATGCTGCTCAGCCTGTTCATGACCCCATACCTAGTCCAACTGTTTCGGCATCACGTAAGAAACAGAAAACGTCACAGTCTGTAGCTTCTTTATCACATGGTGGTGCTCCATCTCCAGTGTTGCCTCCATCCATGCAACCTTCCTCATCAGCCTTGAGACGTGGTCCTCCTCCAGGAGCTCGGAGCAAGAAGTCAAAATCATCCACACAGTTCCCTTCTACAAGTATTACCGGAAGGCCCCAAGCCCCAAATCGAGGTTCTTCAGGTGGCTTTGTGGCAACTGAGCCTGCTGAGGCAACAACTTATGATCCATTAATAGGAAGAAAAGTATGGACAAGGTGGCCTGCGGACAACCATTTCTATGAGGCTGTAATAACTGATTACAACCATATTCAGGGCCGACATGCTCTGGTTTATGATATCAATACAGCGGATGAAACATGGGAATGGGTCAACCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGGTGATGATCCTGGGTTTTCTCGTAGGGGTGGTCGACCTGGATCTGGCCGAGGGACTAAGAAGTCCATCGGACGTGGTGGTGCAGTTTCAGGTGCAGGAAGGGGTAGAGGGACCACAAAAGGTCAGCCTAAGAAGGATTTGCCTCCACAACAAAATGGCATTGGCAGGAAGGCCATGAGTGATATTGAAATACTTCATACGGACTCGTTGATTAAAGAGGTGGAAAAGGTCTTCAGTGCTAGCCATCCTGACCCGATGGAGATTGAGAAAGCTAAGAAAGTTCTCAAAGAGCATGAACAGGCATTGATCGATGCAATTGCAACGCTTGAAGATGCATCAGATGGTGAAAGTGGTAATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

412

Amino Acids

44.93

Weight (kDa)

8.46

Isoelectric Point (pI)

56.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 52 - 120 2.6e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 937
AccB7I CCANNNNNTGG 1 cut(s) 231
AccI GTMKAC 1 cut(s) 910
AciI CCGC 4 cut(s) 64, 199, 731, 815
AclWI GGATC 5 cut(s) 154, 325, 686, 878, 925
AcoI YGGCCR 1 cut(s) 922
AcuI CTGAAG 3 cut(s) 630, 879, 1093
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 7 cut(s) 231, 374, 557, 638, 701, 778, 920
AgsI TTSAA 3 cut(s) 71, 1064, 1205
AjiI CACGTC 2 cut(s) 545, 950
AjnI CCWGG 5 cut(s) 230, 308, 556, 886, 913
AjuI GAANNNNNNNTTGG 2 cut(s) 625, 657
AluBI AGCT 6 cut(s) 13, 110, 271, 470, 563, 1148
AluI AGCT 6 cut(s) 13, 110, 271, 470, 563, 1148
Alw21I GWGCWC 3 cut(s) 15, 493, 565
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 5 cut(s) 154, 325, 686, 878, 925
AoxI GGCC 8 cut(s) 3, 79, 365, 620, 725, 781, 922, 1047
ApeKI GCWGC 2 cut(s) 107, 389
AseI ATTAAT 1 cut(s) 698
Asp700I GAANNNNTTC 2 cut(s) 864, 1068
AspA2I CCTAGG 1 cut(s) 131
AspS9I GGNCC 5 cut(s) 365, 548, 621, 781, 987
AsuC2I CCSGG 1 cut(s) 375
AsuHPI GGTGA 4 cut(s) 155, 220, 890, 1232
AsuNHI GCTAGC 1 cut(s) 1115
AvaII GGWCC 2 cut(s) 548, 987
AvrII CCTAGG 1 cut(s) 131
BanII GRGCYC 2 cut(s) 15, 565
BbsI GAAGAC 1 cut(s) 1099
Bbv12I GWGCWC 3 cut(s) 15, 493, 565
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 2 cut(s) 119, 376
BccI CCATC 8 cut(s) 58, 233, 502, 520, 950, 1126, 1128, 1211
BcgI CGANNNNNNTGC 2 cut(s) 1175, 1209
BciT130I CCWGG 5 cut(s) 232, 310, 558, 888, 915
BcnI CCSGG 1 cut(s) 375
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 3 cut(s) 132, 417, 1116
BfmI CTRYAG 2 cut(s) 105, 465
BglII AGATCT 1 cut(s) 101
BisI GCNGC 2 cut(s) 108, 390
BlnI CCTAGG 1 cut(s) 131
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 2 cut(s) 109, 391
Bme1390I CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
Bme18I GGWCC 2 cut(s) 548, 987
BmgBI CACGTC 2 cut(s) 545, 950
BmgT120I GGNCC 5 cut(s) 365, 548, 621, 781, 987
BmiI GGNNCC 2 cut(s) 623, 988
BmrFI CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
BmsI GCATC 6 cut(s) 126, 211, 443, 1177, 1198, 1220
BmtI GCTAGC 1 cut(s) 1119
BpiI GAAGAC 1 cut(s) 1099
BpmI CTGGAG 2 cut(s) 483, 540
Bpu10I CCTNAGC 1 cut(s) 675
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 559
BpuMI CCSGG 1 cut(s) 375
Bsa29I ATCGAT 1 cut(s) 1185
BsaAI YACGTR 1 cut(s) 440
BsaJI CCNNGG 5 cut(s) 131, 231, 309, 887, 925
BsaWI WCCGGW 1 cut(s) 614
Bsc4I CCNNNNNNNGG 7 cut(s) 231, 374, 557, 638, 701, 778, 920
Bse1I ACTGG 2 cut(s) 360, 500
BseBI CCWGG 5 cut(s) 232, 310, 558, 888, 915
BseCI ATCGAT 1 cut(s) 1185
BseDI CCNNGG 5 cut(s) 131, 231, 309, 887, 925
BseGI GGATG 7 cut(s) 40, 141, 289, 512, 584, 823, 1120
BseLI CCNNNNNNNGG 7 cut(s) 231, 374, 557, 638, 701, 778, 920
BseMII CTCAG 4 cut(s) 263, 407, 657, 666
BseNI ACTGG 2 cut(s) 360, 500
BseRI GAGGAG 2 cut(s) 90, 543
BseXI GCAGC 2 cut(s) 119, 376
BsgI GTGCAG 2 cut(s) 978, 990
BshFI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1049
BshVI ATCGAT 1 cut(s) 1185
BsiHKAI GWGCWC 3 cut(s) 15, 493, 565
BsiSI CCGG 2 cut(s) 374, 615
BslFI GGGAC 2 cut(s) 943, 1000
BslI CCNNNNNNNGG 7 cut(s) 231, 374, 557, 638, 701, 778, 920
BsmAI GTCTC 1 cut(s) 535
BsmBI CGTCTC 1 cut(s) 535
BsmFI GGGAC 2 cut(s) 943, 1000
BsnI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1049
Bsp1286I GDGCHC 3 cut(s) 15, 493, 565
Bsp143I GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
Bsp1720I GCTNAGC 1 cut(s) 393
BspACI CCGC 4 cut(s) 64, 199, 731, 815
BspANI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1049
BspCNI CTCAG 4 cut(s) 264, 406, 658, 667
BspDI ATCGAT 1 cut(s) 1185
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 623, 988
BspMAI CTGCAG 1 cut(s) 109
BspOI GCTAGC 1 cut(s) 1119
BspPI GGATC 5 cut(s) 154, 325, 686, 878, 925
BsrI ACTGG 2 cut(s) 360, 500
BssECI CCNNGG 5 cut(s) 131, 231, 309, 887, 925
BssMI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
BssT1I CCWWGG 1 cut(s) 131
Bst2UI CCWGG 5 cut(s) 232, 310, 558, 888, 915
Bst4CI ACNGT 4 cut(s) 188, 427, 462, 594
BstBAI YACGTR 1 cut(s) 440
BstC8I GCNNGC 4 cut(s) 201, 672, 729, 1117
BstDEI CTNAG 8 cut(s) 260, 272, 393, 666, 675, 933, 1005, 1149
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 7 cut(s) 40, 141, 289, 512, 584, 823, 1120
BstKTI GATC 7 cut(s) 104, 162, 333, 694, 886, 920, 1185
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
BstMWI GCNNNNNNNGC 1 cut(s) 209
BstNI CCWGG 5 cut(s) 232, 310, 558, 888, 915
BstNSI RCATGY 1 cut(s) 791
BstSCI CCNGG 6 cut(s) 230, 308, 373, 556, 886, 913
BstSFI CTRYAG 2 cut(s) 105, 465
BstV1I GCAGC 2 cut(s) 119, 376
BstV2I GAAGAC 1 cut(s) 1099
BstX2I RGATCY 3 cut(s) 101, 159, 917
BstYI RGATCY 3 cut(s) 101, 159, 917
Bsu15I ATCGAT 1 cut(s) 1185
BsuRI GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1049
BsuTUI ATCGAT 1 cut(s) 1185
BtrI CACGTC 2 cut(s) 545, 950
BtsCI GGATG 7 cut(s) 40, 141, 289, 512, 584, 823, 1120
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 4 cut(s) 31, 367, 507, 1117
Cac8I GCNNGC 4 cut(s) 201, 672, 729, 1117
CciI TCATGA 1 cut(s) 403
Cfr13I GGNCC 5 cut(s) 365, 548, 621, 781, 987
ClaI ATCGAT 1 cut(s) 1185
Csp6I GTAC 1 cut(s) 378
CviAII CATG 9 cut(s) 140, 328, 404, 482, 517, 788, 825, 1051, 1169
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 8 cut(s) 260, 272, 393, 666, 675, 933, 1005, 1149
DpnI GATC 7 cut(s) 103, 161, 332, 693, 885, 919, 1184
DpnII GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
DraI TTTAAA 1 cut(s) 208
DraIII CACNNNGTG 1 cut(s) 485
DrdI GACNNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 1 cut(s) 937
EaeI YGGCCR 1 cut(s) 922
Ecl136II GAGCTC 2 cut(s) 13, 563
Eco130I CCWWGG 1 cut(s) 131
Eco24I GRGCYC 2 cut(s) 15, 565
Eco32I GATATC 2 cut(s) 805, 852
Eco47I GGWCC 2 cut(s) 548, 987
Eco53kI GAGCTC 2 cut(s) 13, 563
Eco57I CTGAAG 3 cut(s) 630, 879, 1093
EcoICRI GAGCTC 2 cut(s) 13, 563
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 621
EcoRII CCWGG 5 cut(s) 230, 308, 556, 886, 913
EcoRV GATATC 2 cut(s) 805, 852
EcoT14I CCWWGG 1 cut(s) 131
EcoT22I ATGCAT 2 cut(s) 141, 1213
EcoT38I GRGCYC 2 cut(s) 15, 565
ErhI CCWWGG 1 cut(s) 131
Esp3I CGTCTC 1 cut(s) 535
FaeI CATG 9 cut(s) 143, 331, 407, 485, 520, 791, 828, 1054, 1172
FaqI GGGAC 2 cut(s) 943, 1000
FatI CATG 9 cut(s) 139, 327, 403, 481, 516, 787, 824, 1050, 1168
FauI CCCGC 1 cut(s) 192
FblI GTMKAC 1 cut(s) 910
Fnu4HI GCNGC 2 cut(s) 108, 390
FokI GGATG 7 cut(s) 47, 148, 296, 499, 571, 830, 1107
FriOI GRGCYC 2 cut(s) 15, 565
Fsp4HI GCNGC 2 cut(s) 108, 390
FspBI CTAG 3 cut(s) 132, 417, 1116
GluI GCNGC 2 cut(s) 108, 390
GsuI CTGGAG 2 cut(s) 483, 540
HaeIII GGCC 8 cut(s) 5, 81, 367, 622, 727, 783, 924, 1049
HapII CCGG 2 cut(s) 374, 615
Hin1II CATG 9 cut(s) 143, 331, 407, 485, 520, 791, 828, 1054, 1172
HincII GTYRAC 2 cut(s) 838, 911
HindII GTYRAC 2 cut(s) 838, 911
HinfI GANTC 1 cut(s) 1079
HpaII CCGG 2 cut(s) 374, 615
HphI GGTGA 4 cut(s) 155, 220, 890, 1232
Hpy166II GTNNAC 3 cut(s) 380, 838, 911
Hpy188I TCNGA 7 cut(s) 19, 220, 283, 335, 567, 947, 1216
Hpy188III TCNNGA 3 cut(s) 404, 857, 1124
Hpy8I GTNNAC 3 cut(s) 380, 838, 911
HpyAV CCTTC 9 cut(s) 53, 232, 534, 609, 612, 869, 969, 1003, 1039
HpyCH4III ACNGT 4 cut(s) 188, 427, 462, 594
HpyCH4IV ACGT 4 cut(s) 439, 455, 544, 949
HpyCH4V TGCA 8 cut(s) 107, 139, 520, 959, 971, 1190, 1196, 1211
HpyF10VI GCNNNNNNNGC 1 cut(s) 209
HpyF3I CTNAG 8 cut(s) 260, 272, 393, 666, 675, 933, 1005, 1149
HpySE526I ACGT 4 cut(s) 439, 455, 544, 949
Hsp92II CATG 9 cut(s) 143, 331, 407, 485, 520, 791, 828, 1054, 1172
Kzo9I GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
LmnI GCTCC 5 cut(s) 127, 268, 496, 560, 567
Lsp1109I GCAGC 2 cut(s) 119, 376
LweI GCATC 6 cut(s) 126, 211, 443, 1177, 1198, 1220
MaeI CTAG 3 cut(s) 132, 417, 1116
MaeII ACGT 4 cut(s) 439, 455, 544, 949
MaeIII GTNAC 1 cut(s) 456
MalI GATC 7 cut(s) 103, 161, 332, 693, 885, 919, 1184
MboI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
MboII GAAGA 7 cut(s) 111, 348, 636, 717, 872, 1099, 1217
MfeI CAATTG 1 cut(s) 1191
MflI RGATCY 3 cut(s) 101, 159, 917
MhlI GDGCHC 3 cut(s) 15, 493, 565
MluCI AATT 1 cut(s) 1191
MlyI GAGTC 1 cut(s) 1073
MmeI TCCRAC 1 cut(s) 446
Mph1103I ATGCAT 2 cut(s) 141, 1213
MroXI GAANNNNTTC 2 cut(s) 864, 1068
MseI TTAA 4 cut(s) 207, 248, 698, 1089
MslI CAYNNNNRTG 3 cut(s) 486, 744, 829
MspA1I CMGCKG 2 cut(s) 110, 815
MspI CCGG 2 cut(s) 374, 615
MspR9I CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
MunI CAATTG 1 cut(s) 1191
MvaI CCWGG 5 cut(s) 232, 310, 558, 888, 915
MwoI GCNNNNNNNGC 1 cut(s) 209
NciI CCSGG 1 cut(s) 375
NdeII GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
NheI GCTAGC 1 cut(s) 1115
NlaIII CATG 9 cut(s) 143, 331, 407, 485, 520, 791, 828, 1054, 1172
NlaIV GGNNCC 2 cut(s) 623, 988
NmeAIII GCCGAG 1 cut(s) 950
NmuCI GTSAC 1 cut(s) 456
NsiI ATGCAT 2 cut(s) 141, 1213
NspI RCATGY 1 cut(s) 791
PagI TCATGA 1 cut(s) 403
PdmI GAANNNNTTC 2 cut(s) 864, 1068
PflFI GACNNNGTC 1 cut(s) 546
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 1 cut(s) 556
PkrI GCNGC 2 cut(s) 109, 391
PleI GAGTC 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 1073
Ppu21I YACGTR 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 698
Psp124BI GAGCTC 2 cut(s) 15, 565
Psp6I CCWGG 5 cut(s) 230, 308, 556, 886, 913
PspGI CCWGG 5 cut(s) 230, 308, 556, 886, 913
PspN4I GGNNCC 2 cut(s) 623, 988
PspPI GGNCC 5 cut(s) 365, 548, 621, 781, 987
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 3 cut(s) 101, 159, 917
PsyI GACNNNGTC 1 cut(s) 546
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 3 cut(s) 486, 744, 829
SacI GAGCTC 2 cut(s) 15, 565
SalI GTCGAC 1 cut(s) 909
SaqAI TTAA 4 cut(s) 207, 248, 698, 1089
SatI GCNGC 2 cut(s) 108, 390
Sau3AI GATC 7 cut(s) 101, 159, 330, 691, 883, 917, 1182
Sau96I GGNCC 5 cut(s) 365, 548, 621, 781, 987
SchI GAGTC 1 cut(s) 1073
ScrFI CCNGG 6 cut(s) 232, 310, 375, 558, 888, 915
SduI GDGCHC 3 cut(s) 15, 493, 565
SfaNI GCATC 6 cut(s) 126, 211, 443, 1177, 1198, 1220
SfcI CTRYAG 2 cut(s) 105, 465
SinI GGWCC 2 cut(s) 548, 987
SmiMI CAYNNNNRTG 3 cut(s) 486, 744, 829
SmlI CTYRAG 1 cut(s) 538
SmoI CTYRAG 1 cut(s) 538
Sse9I AATT 1 cut(s) 1191
SsiI CCGC 4 cut(s) 64, 199, 731, 815
SspMI CTAG 3 cut(s) 132, 417, 1116
SstI GAGCTC 2 cut(s) 15, 565
StyD4I CCNGG 6 cut(s) 230, 308, 373, 556, 886, 913
StyI CCWWGG 1 cut(s) 131
TaaI ACNGT 4 cut(s) 188, 427, 462, 594
TaiI ACGT 4 cut(s) 442, 458, 547, 952
TaqI TCGA 3 cut(s) 637, 910, 1185
TasI AATT 1 cut(s) 1191
Tru1I TTAA 4 cut(s) 207, 248, 698, 1089
Tru9I TTAA 4 cut(s) 207, 248, 698, 1089
TscAI CASTG 4 cut(s) 31, 367, 507, 1117
TseFI GTSAC 1 cut(s) 456
TseI GCWGC 2 cut(s) 107, 389
Tsp45I GTSAC 1 cut(s) 456
TspDTI ATGAA 4 cut(s) 392, 834, 1061, 1185
TspGWI ACGGA 3 cut(s) 47, 269, 1091
TspRI CASTG 4 cut(s) 31, 367, 507, 1117
Tth111I GACNNNGTC 1 cut(s) 546
Van91I CCANNNNNTGG 1 cut(s) 231
VpaK11BI GGWCC 2 cut(s) 548, 987
VspI ATTAAT 1 cut(s) 698
XagI CCTNNNNNAGG 1 cut(s) 555
XceI RCATGY 1 cut(s) 791
XmaJI CCTAGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 910
XmnI GAANNNNTTC 2 cut(s) 864, 1068
XspI CTAG 3 cut(s) 132, 417, 1116
Zsp2I ATGCAT 2 cut(s) 141, 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.