Rh5DG554300

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0007058
Physical Location & Seq
Forward (+)
85613443 .. 85618837
5395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG554300.1

Sequence Viewer

Length: 1266 bp
ATGCATGGTGATATGGAAACACAGATCCACCACATTGAGCAAGAAGCATACTGTTCAGTCCTGCGGGCATTTAAAGCCCAATCTGATGCCATCACCTGGGAGAAGGAAAGTTTAATAACGGAACTTAGAAAGGAGCTGAGAGTATCGGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCGAAGAATAAGGGAGTGGAGAAAAACCAGTGGGCCTCAGCCGGGTACACATAATGCTGCTCAGCCTGTTCATGACCCCATACCTAGTCCAACTGTTTCGGCATCACGTAAGAAACAGAAAACGTCACAGTCTGTAGCTTCTTTATCACATGGTGGTGCTCCATCTCCAGTGCTGCCTCCATCCATGCAACCTTCCTCATCAGCCTTGAGACGTGGTCCTCCTCCAGGAGCTCGGAGCAAGAAGTCAAAATCATCCACACAGTTCCCTTCTACAAGTATTACCGGAAGGCCCCAAGCCCCAAATCGAGGTTCTTCAGGTGGCTTTGTGGCAACTGAGCCTGCTGAGGCAACAACTTATGATCCATTAATAGGAAGAAAAGTATGGACAAGGTGGCCTGCAGACAACCATTTCTATGAGGCTGTAATAACTGATTACAACCATATTCAGGGCCGACATGCTCTGGTTTATGATATCAATACAGCGGATGAAACATGGGAATGGGTCAACCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGGTGATGATCCTGGGTTTTCTCGTAGGGGTGGTCGACCTGGATCTGGCCGAGGGACTAAGAAGTCCATTGGACGTGGTGGTGCAGTTTCAGGTGCAGGAAGGGGTAGAGGGACCACAAAAGGTCAGCCTAAGAAGGATTTGCCTCCACAACAAAATGGCATTGGCAGGAAGGCCATGAGCGACATTGAAATACTTCATACAGACTCGTTGATTAAAGAGGTGGAAAAGGTCTTCAGTGCTAGCCATCCTGACCCGATGGAGATTGAGAAAGCTAAGAAAGTTCTCAAAGAGCATGAACAGGCATTGATCGATGCAATTGCAACGCTTGAAGATGCATCAGATGGTGAAAGTGATGAACATACATTCTCTCAAAGACAACCAATGGACCAAGAACGAGGATGGAGAAAACGGCAATATGATGAAATGGCAGAAGGTCAGGCAGTTGATGGTTCAAATGGTACTAAAATCATGAGAGATGGTAGAATTGCATCTGATGACCAACGTTTTGAAGGCGATGGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

421

Amino Acids

46.55

Weight (kDa)

6.73

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 7 - 75 2.7e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 802
AccB7I CCANNNNNTGG 1 cut(s) 96
AccI GTMKAC 1 cut(s) 775
AciI CCGC 2 cut(s) 64, 680
AclI AACGTT 1 cut(s) 1243
AclWI GGATC 5 cut(s) 19, 190, 551, 743, 790
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 495, 744, 958
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 244, 1201
AfiI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
AgsI TTSAA 4 cut(s) 929, 1070, 1194, 1250
AjiI CACGTC 2 cut(s) 410, 815
AjnI CCWGG 5 cut(s) 95, 173, 421, 751, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AluBI AGCT 4 cut(s) 136, 335, 428, 1013
AluI AGCT 4 cut(s) 136, 335, 428, 1013
Alw21I GWGCWC 2 cut(s) 358, 430
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 5 cut(s) 19, 190, 551, 743, 790
AoxI GGCC 6 cut(s) 230, 485, 590, 646, 787, 912
ApeKI GCWGC 2 cut(s) 254, 370
ArsI GACNNNNNNTTYG 2 cut(s) 1229, 1261
AseI ATTAAT 1 cut(s) 563
Asp700I GAANNNNTTC 2 cut(s) 729, 933
AspS9I GGNCC 6 cut(s) 230, 413, 486, 646, 852, 1126
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 4 cut(s) 20, 85, 755, 1097
AsuNHI GCTAGC 1 cut(s) 980
AvaII GGWCC 3 cut(s) 413, 852, 1126
BanII GRGCYC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 964
Bbv12I GWGCWC 2 cut(s) 358, 430
BbvCI CCTCAGC 2 cut(s) 234, 540
BbvI GCAGC 2 cut(s) 241, 357
BceAI ACGGC 1 cut(s) 1166
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 5 cut(s) 97, 175, 423, 753, 780
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 282, 981
BfmI CTRYAG 2 cut(s) 330, 594
BisI GCNGC 2 cut(s) 255, 371
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 256, 372
Bme1390I CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
Bme18I GGWCC 3 cut(s) 413, 852, 1126
BmgBI CACGTC 2 cut(s) 410, 815
BmgT120I GGNCC 6 cut(s) 230, 413, 486, 646, 852, 1126
BmiI GGNNCC 2 cut(s) 488, 853
BmrFI CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
BmsI GCATC 6 cut(s) 76, 308, 1042, 1063, 1085, 1238
BmtI GCTAGC 1 cut(s) 984
BpiI GAAGAC 1 cut(s) 964
BpmI CTGGAG 2 cut(s) 348, 405
Bpu10I CCTNAGC 2 cut(s) 234, 540
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 1050
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 96, 174, 752, 790
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
Bse1I ACTGG 2 cut(s) 225, 365
BseBI CCWGG 5 cut(s) 97, 175, 423, 753, 780
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 4 cut(s) 96, 174, 752, 790
BseGI GGATG 6 cut(s) 154, 377, 449, 688, 985, 1145
BseLI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
BseMII CTCAG 5 cut(s) 128, 248, 272, 522, 531
BseNI ACTGG 2 cut(s) 225, 365
BseRI GAGGAG 1 cut(s) 408
BseXI GCAGC 2 cut(s) 241, 357
BsgI GTGCAG 2 cut(s) 843, 855
BshFI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BshVI ATCGAT 1 cut(s) 1050
BsiHKAI GWGCWC 2 cut(s) 358, 430
BsiSI CCGG 2 cut(s) 239, 480
BslFI GGGAC 2 cut(s) 808, 865
BslI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
BsmAI GTCTC 1 cut(s) 400
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 2 cut(s) 808, 865
BsnI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
Bsp1286I GDGCHC 2 cut(s) 358, 430
Bsp143I GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Bsp1720I GCTNAGC 1 cut(s) 258
BspACI CCGC 2 cut(s) 64, 680
BspANI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BspCNI CTCAG 5 cut(s) 129, 247, 271, 523, 532
BspDI ATCGAT 1 cut(s) 1050
BspHI TCATGA 2 cut(s) 268, 1209
BspLI GGNNCC 2 cut(s) 488, 853
BspMAI CTGCAG 1 cut(s) 598
BspOI GCTAGC 1 cut(s) 984
BspPI GGATC 5 cut(s) 19, 190, 551, 743, 790
BsrI ACTGG 2 cut(s) 225, 365
BssECI CCNNGG 4 cut(s) 96, 174, 752, 790
BssMI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Bst2UI CCWGG 5 cut(s) 97, 175, 423, 753, 780
Bst4CI ACNGT 4 cut(s) 53, 292, 327, 459
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 66, 537, 594, 982
BstDEI CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 6 cut(s) 154, 377, 449, 688, 985, 1145
BstKTI GATC 6 cut(s) 27, 198, 559, 751, 785, 1050
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 5 cut(s) 97, 175, 423, 753, 780
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 95, 173, 238, 421, 751, 778
BstSFI CTRYAG 2 cut(s) 330, 594
BstV1I GCAGC 2 cut(s) 241, 357
BstV2I GAAGAC 1 cut(s) 964
BstX2I RGATCY 2 cut(s) 24, 782
BstYI RGATCY 2 cut(s) 24, 782
Bsu15I ATCGAT 1 cut(s) 1050
BsuRI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BsuTUI ATCGAT 1 cut(s) 1050
BtrI CACGTC 2 cut(s) 410, 815
BtsCI GGATG 6 cut(s) 154, 377, 449, 688, 985, 1145
BtsIMutI CAGTG 3 cut(s) 232, 372, 982
Cac8I GCNNGC 4 cut(s) 66, 537, 594, 982
CciI TCATGA 2 cut(s) 268, 1209
Cfr13I GGNCC 6 cut(s) 230, 413, 486, 646, 852, 1126
ClaI ATCGAT 1 cut(s) 1050
Csp6I GTAC 2 cut(s) 243, 1200
CviQI GTAC 2 cut(s) 243, 1200
DdeI CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
DpnI GATC 6 cut(s) 26, 197, 558, 750, 784, 1049
DpnII GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 350
DrdI GACNNNNNNGTC 1 cut(s) 802
DseDI GACNNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 787
Ecl136II GAGCTC 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 430
Eco32I GATATC 2 cut(s) 670, 717
Eco47I GGWCC 3 cut(s) 413, 852, 1126
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 3 cut(s) 495, 744, 958
EcoICRI GAGCTC 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 486
EcoRII CCWGG 5 cut(s) 95, 173, 421, 751, 778
EcoRV GATATC 2 cut(s) 670, 717
EcoT22I ATGCAT 2 cut(s) 6, 1078
EcoT38I GRGCYC 1 cut(s) 430
Esp3I CGTCTC 1 cut(s) 400
FaqI GGGAC 2 cut(s) 808, 865
FauI CCCGC 1 cut(s) 57
FauNDI CATATG 1 cut(s) 1262
FblI GTMKAC 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 255, 371
FokI GGATG 6 cut(s) 161, 364, 436, 695, 972, 1152
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 2 cut(s) 255, 371
FspBI CTAG 2 cut(s) 282, 981
GluI GCNGC 2 cut(s) 255, 371
GsuI CTGGAG 2 cut(s) 348, 405
HaeIII GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
HapII CCGG 2 cut(s) 239, 480
HincII GTYRAC 2 cut(s) 703, 776
HindII GTYRAC 2 cut(s) 703, 776
HinfI GANTC 1 cut(s) 944
HpaII CCGG 2 cut(s) 239, 480
HphI GGTGA 4 cut(s) 20, 85, 755, 1097
Hpy166II GTNNAC 3 cut(s) 245, 703, 776
Hpy188I TCNGA 6 cut(s) 85, 148, 200, 432, 1081, 1234
Hpy188III TCNNGA 4 cut(s) 269, 722, 989, 1210
Hpy8I GTNNAC 3 cut(s) 245, 703, 776
HpyCH4III ACNGT 4 cut(s) 53, 292, 327, 459
HpyCH4IV ACGT 5 cut(s) 304, 320, 409, 814, 1243
HpyCH4V TGCA 9 cut(s) 4, 385, 596, 824, 836, 1055, 1061, 1076, 1229
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
HpySE526I ACGT 5 cut(s) 304, 320, 409, 814, 1243
Kzo9I GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
LmnI GCTCC 4 cut(s) 133, 361, 425, 432
Lsp1109I GCAGC 2 cut(s) 241, 357
LweI GCATC 6 cut(s) 76, 308, 1042, 1063, 1085, 1238
MaeI CTAG 2 cut(s) 282, 981
MaeII ACGT 5 cut(s) 304, 320, 409, 814, 1243
MaeIII GTNAC 1 cut(s) 321
MalI GATC 6 cut(s) 26, 197, 558, 750, 784, 1049
MboI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
MboII GAAGA 6 cut(s) 213, 501, 582, 737, 964, 1082
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 2 cut(s) 24, 782
MhlI GDGCHC 2 cut(s) 358, 430
MluCI AATT 2 cut(s) 1056, 1224
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 1 cut(s) 311
Mph1103I ATGCAT 2 cut(s) 6, 1078
MroXI GAANNNNTTC 2 cut(s) 729, 933
MseI TTAA 4 cut(s) 72, 113, 563, 954
MslI CAYNNNNRTG 3 cut(s) 351, 609, 694
MspA1I CMGCKG 1 cut(s) 680
MspI CCGG 2 cut(s) 239, 480
MspR9I CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
MunI CAATTG 1 cut(s) 1056
MvaI CCWGG 5 cut(s) 97, 175, 423, 753, 780
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 1262
NdeII GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
NheI GCTAGC 1 cut(s) 980
NlaIV GGNNCC 2 cut(s) 488, 853
NmeAIII GCCGAG 1 cut(s) 815
NmuCI GTSAC 1 cut(s) 321
NsiI ATGCAT 2 cut(s) 6, 1078
NspI RCATGY 1 cut(s) 656
PagI TCATGA 2 cut(s) 268, 1209
PdmI GAANNNNTTC 2 cut(s) 729, 933
PflFI GACNNNGTC 1 cut(s) 411
PflMI CCANNNNNTGG 1 cut(s) 96
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 2 cut(s) 256, 372
PleI GAGTC 1 cut(s) 938
PpsI GAGTC 1 cut(s) 938
Ppu21I YACGTR 1 cut(s) 305
PshBI ATTAAT 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 430
Psp1406I AACGTT 1 cut(s) 1243
Psp6I CCWGG 5 cut(s) 95, 173, 421, 751, 778
PspGI CCWGG 5 cut(s) 95, 173, 421, 751, 778
PspN4I GGNNCC 2 cut(s) 488, 853
PspPI GGNCC 6 cut(s) 230, 413, 486, 646, 852, 1126
PstI CTGCAG 1 cut(s) 598
PsuI RGATCY 2 cut(s) 24, 782
PsyI GACNNNGTC 1 cut(s) 411
RsaI GTAC 2 cut(s) 244, 1201
RsaNI GTAC 2 cut(s) 243, 1200
RseI CAYNNNNRTG 3 cut(s) 351, 609, 694
SacI GAGCTC 1 cut(s) 430
SalI GTCGAC 1 cut(s) 774
SaqAI TTAA 4 cut(s) 72, 113, 563, 954
SatI GCNGC 2 cut(s) 255, 371
Sau3AI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Sau96I GGNCC 6 cut(s) 230, 413, 486, 646, 852, 1126
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
SduI GDGCHC 2 cut(s) 358, 430
SfaNI GCATC 6 cut(s) 76, 308, 1042, 1063, 1085, 1238
SfcI CTRYAG 2 cut(s) 330, 594
SinI GGWCC 3 cut(s) 413, 852, 1126
SmiMI CAYNNNNRTG 3 cut(s) 351, 609, 694
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 2 cut(s) 1056, 1224
SsiI CCGC 2 cut(s) 64, 680
SspMI CTAG 2 cut(s) 282, 981
SstI GAGCTC 1 cut(s) 430
StyD4I CCNGG 6 cut(s) 95, 173, 238, 421, 751, 778
TaaI ACNGT 4 cut(s) 53, 292, 327, 459
TaiI ACGT 5 cut(s) 307, 323, 412, 817, 1246
TaqI TCGA 3 cut(s) 502, 775, 1050
TasI AATT 2 cut(s) 1056, 1224
Tru1I TTAA 4 cut(s) 72, 113, 563, 954
Tru9I TTAA 4 cut(s) 72, 113, 563, 954
TscAI CASTG 3 cut(s) 232, 372, 982
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 254, 370
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 6 cut(s) 257, 699, 926, 1050, 1110, 1176
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 3 cut(s) 232, 372, 982
Tth111I GACNNNGTC 1 cut(s) 411
Van91I CCANNNNNTGG 1 cut(s) 96
VpaK11BI GGWCC 3 cut(s) 413, 852, 1126
VspI ATTAAT 1 cut(s) 563
XagI CCTNNNNNAGG 1 cut(s) 420
XceI RCATGY 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 775
XmnI GAANNNNTTC 2 cut(s) 729, 933
XspI CTAG 2 cut(s) 282, 981
Zsp2I ATGCAT 2 cut(s) 6, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.