Rh5BG544000

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0007058
Physical Location & Seq
Forward (+)
86643986 .. 86649303
5318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG544000.1

Sequence Viewer

Length: 1266 bp
ATGCATGGTGATATGGAAACACAGATCCACCACATTGAGCAAGAAGCATACTGTTCAGTCCTGCGGGCATTTAAAGCCCAATCTGATGCCATCACCTGGGAGAAGGAAAGTTTAATAACGGAACTTAGAAAGGAGCTGAGAGTATCGGATGAGGAACACAGAGAACTTTTGTCCAGGGTCAATGCCGATGACATGATCCGAAGAATAAGGGAGTGGAGAAAAACCAGTGGGCCTCAGCCGGGTACACATAATGCCGCTCAGCCTGTTCATGACCCCATACCTAGTCCAACTGTTTCGGCATCACGTAAGAAACAGAAAACATCACAGTCTGTAGCTTCTTTATCACATGGTGGTGCTCCATCTCCAGTGCTGCCTCCATCCATGCAACCTTCCTCATCAGCCTTGAGACGTGGTCCTCCTCCAGGAGCTCGGAGCAAGAAGTCAAAATCATCCACACAGTTCCCTTCTACAAGTATTACCGGAAGGCCCCAAGCCCCAAACCGAGGTTCTTCAGGTGGCTTTGTGGCAACTGAGCCTGCTGAGGCAACAACTTATGATCCATTAATAGGAAGAAAAGTATGGACAAGGTGGCCCGCGGACAACCATTTCTATGAGGCTGTAATAACTGATTACAACCATATTCAGGGCCGACATGCTCTGGTTTATGATATCAATACAGCGGATGAAACATGGGAATGGGTCAACCTAAAAGAGATATCTCCTGAAGATATTCGGTGGGAAGGTGATGATCCTGGGTTTTCTCGTAGGGGTGGTCGACCTGGATCTGGCCGAGGGACTAAGAAGTCCATGGGACGTGGTGGTGCAGTTTCAGGTGCAGGAAGGGGTAGAGGGACCACAAAAGGTCAGCCTAAGAAGGATTTGCCTCCACAACAAAATGGCATTGGCAGGAAGGCCATGAGCGACATTGAAATACTTCATACAGACTCGTTGATTAAAGAGGTGGAAAAGGTCTTCAGTGCTAGCCATCCTGACCCGATGGAGATTGAGAAAGCTAAGAAAGTTCTCAAAGAGCATGAACAGGCATTGATCGATGCAATTGCAACGCTTGAAGATGCATCAGATGGTGAAAGTGATGAACATACATTCTCTCAAAGACAACCAATGGACCAAGAACGAGGATGGAGAAAACGGCAATATGATGAAATGGCAGAAGGTCAGGCAGTTGATGGTTCAAATGGTACTAAAATCATGAGAGATGGTAGAATTGCATCTGATGACCAACGTTTTGAAGGCGATGGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

421

Amino Acids

46.56

Weight (kDa)

6.73

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 7 - 75 2.7e-31 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 802
AccB7I CCANNNNNTGG 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 257
AccI GTMKAC 1 cut(s) 775
AccII CGCG 1 cut(s) 596
AciI CCGC 5 cut(s) 64, 255, 594, 596, 680
AclI AACGTT 1 cut(s) 1243
AclWI GGATC 5 cut(s) 19, 190, 551, 743, 790
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 3 cut(s) 495, 744, 958
AdeI CACNNNGTG 1 cut(s) 350
AfaI GTAC 2 cut(s) 244, 1201
AfiI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
AgsI TTSAA 4 cut(s) 929, 1070, 1194, 1250
AjiI CACGTC 2 cut(s) 410, 815
AjnI CCWGG 5 cut(s) 95, 173, 421, 751, 778
AjuI GAANNNNNNNTTGG 2 cut(s) 490, 522
AluBI AGCT 4 cut(s) 136, 335, 428, 1013
AluI AGCT 4 cut(s) 136, 335, 428, 1013
Alw21I GWGCWC 2 cut(s) 358, 430
Alw26I GTCTC 1 cut(s) 400
AlwI GGATC 5 cut(s) 19, 190, 551, 743, 790
AoxI GGCC 6 cut(s) 230, 485, 590, 646, 787, 912
ApeKI GCWGC 1 cut(s) 370
ArsI GACNNNNNNTTYG 2 cut(s) 1229, 1261
AseI ATTAAT 1 cut(s) 563
Asp700I GAANNNNTTC 2 cut(s) 729, 933
AspS9I GGNCC 7 cut(s) 230, 413, 486, 591, 646, 852, 1126
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 4 cut(s) 20, 85, 755, 1097
AsuNHI GCTAGC 1 cut(s) 980
AvaII GGWCC 3 cut(s) 413, 852, 1126
BanII GRGCYC 1 cut(s) 430
BbsI GAAGAC 1 cut(s) 964
Bbv12I GWGCWC 2 cut(s) 358, 430
BbvCI CCTCAGC 2 cut(s) 234, 540
BbvI GCAGC 1 cut(s) 357
BceAI ACGGC 1 cut(s) 1166
BcgI CGANNNNNNTGC 2 cut(s) 1040, 1074
BciT130I CCWGG 5 cut(s) 97, 175, 423, 753, 780
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 1 cut(s) 400
BfaI CTAG 2 cut(s) 282, 981
BfmI CTRYAG 1 cut(s) 330
BisI GCNGC 2 cut(s) 255, 371
BlpI GCTNAGC 1 cut(s) 258
BlsI GCNGC 2 cut(s) 256, 372
Bme1390I CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
Bme18I GGWCC 3 cut(s) 413, 852, 1126
BmgBI CACGTC 2 cut(s) 410, 815
BmgT120I GGNCC 7 cut(s) 230, 413, 486, 591, 646, 852, 1126
BmiI GGNNCC 2 cut(s) 488, 853
BmrFI CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
BmsI GCATC 6 cut(s) 76, 308, 1042, 1063, 1085, 1238
BmtI GCTAGC 1 cut(s) 984
BpiI GAAGAC 1 cut(s) 964
BpmI CTGGAG 2 cut(s) 348, 405
Bpu10I CCTNAGC 2 cut(s) 234, 540
Bpu1102I GCTNAGC 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 424
BpuMI CCSGG 1 cut(s) 240
Bsa29I ATCGAT 1 cut(s) 1050
BsaAI YACGTR 1 cut(s) 305
BsaJI CCNNGG 7 cut(s) 96, 174, 502, 594, 752, 790, 807
BsaWI WCCGGW 1 cut(s) 479
Bsc4I CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
Bse1I ACTGG 2 cut(s) 225, 365
BseBI CCWGG 5 cut(s) 97, 175, 423, 753, 780
BseCI ATCGAT 1 cut(s) 1050
BseDI CCNNGG 7 cut(s) 96, 174, 502, 594, 752, 790, 807
BseGI GGATG 6 cut(s) 154, 377, 449, 688, 985, 1145
BseLI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
BseMII CTCAG 5 cut(s) 128, 248, 272, 522, 531
BseNI ACTGG 2 cut(s) 225, 365
BseRI GAGGAG 1 cut(s) 408
BseXI GCAGC 1 cut(s) 357
BsgI GTGCAG 2 cut(s) 843, 855
Bsh1236I CGCG 1 cut(s) 596
BshFI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BshVI ATCGAT 1 cut(s) 1050
BsiHKAI GWGCWC 2 cut(s) 358, 430
BsiSI CCGG 2 cut(s) 239, 480
BslFI GGGAC 3 cut(s) 808, 825, 865
BslI CCNNNNNNNGG 7 cut(s) 96, 239, 422, 503, 566, 643, 785
BsmAI GTCTC 1 cut(s) 400
BsmBI CGTCTC 1 cut(s) 400
BsmFI GGGAC 3 cut(s) 808, 825, 865
BsnI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
Bsp1286I GDGCHC 2 cut(s) 358, 430
Bsp143I GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Bsp1720I GCTNAGC 1 cut(s) 258
Bsp19I CCATGG 1 cut(s) 807
BspACI CCGC 5 cut(s) 64, 255, 594, 596, 680
BspANI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BspCNI CTCAG 5 cut(s) 129, 247, 271, 523, 532
BspDI ATCGAT 1 cut(s) 1050
BspFNI CGCG 1 cut(s) 596
BspHI TCATGA 2 cut(s) 268, 1209
BspLI GGNNCC 2 cut(s) 488, 853
BspOI GCTAGC 1 cut(s) 984
BspPI GGATC 5 cut(s) 19, 190, 551, 743, 790
BsrBI CCGCTC 1 cut(s) 257
BsrI ACTGG 2 cut(s) 225, 365
BssECI CCNNGG 7 cut(s) 96, 174, 502, 594, 752, 790, 807
BssMI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
BssT1I CCWWGG 1 cut(s) 807
Bst2UI CCWGG 5 cut(s) 97, 175, 423, 753, 780
Bst4CI ACNGT 4 cut(s) 53, 292, 327, 459
BstBAI YACGTR 1 cut(s) 305
BstC8I GCNNGC 4 cut(s) 66, 537, 594, 982
BstDEI CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
BstDSI CCRYGG 2 cut(s) 594, 807
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 6 cut(s) 154, 377, 449, 688, 985, 1145
BstFNI CGCG 1 cut(s) 596
BstKTI GATC 6 cut(s) 27, 198, 559, 751, 785, 1050
BstMAI GTCTC 1 cut(s) 400
BstMBI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
BstMWI GCNNNNNNNGC 1 cut(s) 74
BstNI CCWGG 5 cut(s) 97, 175, 423, 753, 780
BstNSI RCATGY 1 cut(s) 656
BstSCI CCNGG 6 cut(s) 95, 173, 238, 421, 751, 778
BstSFI CTRYAG 1 cut(s) 330
BstUI CGCG 1 cut(s) 596
BstV1I GCAGC 1 cut(s) 357
BstV2I GAAGAC 1 cut(s) 964
BstX2I RGATCY 2 cut(s) 24, 782
BstYI RGATCY 2 cut(s) 24, 782
Bsu15I ATCGAT 1 cut(s) 1050
BsuRI GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
BsuTUI ATCGAT 1 cut(s) 1050
BtgI CCRYGG 2 cut(s) 594, 807
BtrI CACGTC 2 cut(s) 410, 815
BtsCI GGATG 6 cut(s) 154, 377, 449, 688, 985, 1145
BtsIMutI CAGTG 3 cut(s) 232, 372, 982
Cac8I GCNNGC 4 cut(s) 66, 537, 594, 982
CciI TCATGA 2 cut(s) 268, 1209
Cfr13I GGNCC 7 cut(s) 230, 413, 486, 591, 646, 852, 1126
Cfr42I CCGCGG 1 cut(s) 597
ClaI ATCGAT 1 cut(s) 1050
Csp6I GTAC 2 cut(s) 243, 1200
CviQI GTAC 2 cut(s) 243, 1200
DdeI CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
DpnI GATC 6 cut(s) 26, 197, 558, 750, 784, 1049
DpnII GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
DraI TTTAAA 1 cut(s) 73
DraIII CACNNNGTG 1 cut(s) 350
DrdI GACNNNNNNGTC 1 cut(s) 802
DseDI GACNNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 787
Ecl136II GAGCTC 1 cut(s) 428
Eco130I CCWWGG 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 430
Eco32I GATATC 2 cut(s) 670, 717
Eco47I GGWCC 3 cut(s) 413, 852, 1126
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 3 cut(s) 495, 744, 958
EcoICRI GAGCTC 1 cut(s) 428
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoO109I RGGNCCY 1 cut(s) 486
EcoRII CCWGG 5 cut(s) 95, 173, 421, 751, 778
EcoRV GATATC 2 cut(s) 670, 717
EcoT14I CCWWGG 1 cut(s) 807
EcoT22I ATGCAT 2 cut(s) 6, 1078
EcoT38I GRGCYC 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 807
Esp3I CGTCTC 1 cut(s) 400
FaqI GGGAC 3 cut(s) 808, 825, 865
FauI CCCGC 2 cut(s) 57, 601
FauNDI CATATG 1 cut(s) 1262
FblI GTMKAC 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 255, 371
FokI GGATG 6 cut(s) 161, 364, 436, 695, 972, 1152
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 2 cut(s) 255, 371
FspBI CTAG 2 cut(s) 282, 981
GluI GCNGC 2 cut(s) 255, 371
GsuI CTGGAG 2 cut(s) 348, 405
HaeIII GGCC 6 cut(s) 232, 487, 592, 648, 789, 914
HapII CCGG 2 cut(s) 239, 480
HincII GTYRAC 2 cut(s) 703, 776
HindII GTYRAC 2 cut(s) 703, 776
HinfI GANTC 1 cut(s) 944
HpaII CCGG 2 cut(s) 239, 480
HphI GGTGA 4 cut(s) 20, 85, 755, 1097
Hpy166II GTNNAC 3 cut(s) 245, 703, 776
Hpy188I TCNGA 6 cut(s) 85, 148, 200, 432, 1081, 1234
Hpy188III TCNNGA 4 cut(s) 269, 722, 989, 1210
Hpy8I GTNNAC 3 cut(s) 245, 703, 776
HpyCH4III ACNGT 4 cut(s) 53, 292, 327, 459
HpyCH4IV ACGT 4 cut(s) 304, 409, 814, 1243
HpyCH4V TGCA 8 cut(s) 4, 385, 824, 836, 1055, 1061, 1076, 1229
HpyF10VI GCNNNNNNNGC 1 cut(s) 74
HpyF3I CTNAG 9 cut(s) 125, 137, 234, 258, 531, 540, 798, 870, 1014
HpySE526I ACGT 4 cut(s) 304, 409, 814, 1243
KspI CCGCGG 1 cut(s) 597
Kzo9I GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
LmnI GCTCC 4 cut(s) 133, 361, 425, 432
Lsp1109I GCAGC 1 cut(s) 357
LweI GCATC 6 cut(s) 76, 308, 1042, 1063, 1085, 1238
MaeI CTAG 2 cut(s) 282, 981
MaeII ACGT 4 cut(s) 304, 409, 814, 1243
MalI GATC 6 cut(s) 26, 197, 558, 750, 784, 1049
MbiI CCGCTC 1 cut(s) 257
MboI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
MboII GAAGA 6 cut(s) 213, 501, 582, 737, 964, 1082
MfeI CAATTG 1 cut(s) 1056
MflI RGATCY 2 cut(s) 24, 782
MhlI GDGCHC 2 cut(s) 358, 430
MluCI AATT 2 cut(s) 1056, 1224
MlyI GAGTC 1 cut(s) 938
MmeI TCCRAC 1 cut(s) 311
Mph1103I ATGCAT 2 cut(s) 6, 1078
MroXI GAANNNNTTC 2 cut(s) 729, 933
MseI TTAA 4 cut(s) 72, 113, 563, 954
MslI CAYNNNNRTG 3 cut(s) 351, 609, 694
MspA1I CMGCKG 2 cut(s) 596, 680
MspI CCGG 2 cut(s) 239, 480
MspR9I CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
MunI CAATTG 1 cut(s) 1056
MvaI CCWGG 5 cut(s) 97, 175, 423, 753, 780
MvnI CGCG 1 cut(s) 596
MwoI GCNNNNNNNGC 1 cut(s) 74
NciI CCSGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 807
NdeI CATATG 1 cut(s) 1262
NdeII GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
NheI GCTAGC 1 cut(s) 980
NlaIV GGNNCC 2 cut(s) 488, 853
NmeAIII GCCGAG 1 cut(s) 815
NsiI ATGCAT 2 cut(s) 6, 1078
NspI RCATGY 1 cut(s) 656
PagI TCATGA 2 cut(s) 268, 1209
PdmI GAANNNNTTC 2 cut(s) 729, 933
PflFI GACNNNGTC 1 cut(s) 411
PflMI CCANNNNNTGG 1 cut(s) 96
PfoI TCCNGGA 1 cut(s) 421
PkrI GCNGC 2 cut(s) 256, 372
PleI GAGTC 1 cut(s) 938
PpsI GAGTC 1 cut(s) 938
Ppu21I YACGTR 1 cut(s) 305
PshBI ATTAAT 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 430
Psp1406I AACGTT 1 cut(s) 1243
Psp6I CCWGG 5 cut(s) 95, 173, 421, 751, 778
PspGI CCWGG 5 cut(s) 95, 173, 421, 751, 778
PspN4I GGNNCC 2 cut(s) 488, 853
PspPI GGNCC 7 cut(s) 230, 413, 486, 591, 646, 852, 1126
PsuI RGATCY 2 cut(s) 24, 782
PsyI GACNNNGTC 1 cut(s) 411
RsaI GTAC 2 cut(s) 244, 1201
RsaNI GTAC 2 cut(s) 243, 1200
RseI CAYNNNNRTG 3 cut(s) 351, 609, 694
SacI GAGCTC 1 cut(s) 430
SacII CCGCGG 1 cut(s) 597
SalI GTCGAC 1 cut(s) 774
SaqAI TTAA 4 cut(s) 72, 113, 563, 954
SatI GCNGC 2 cut(s) 255, 371
Sau3AI GATC 6 cut(s) 24, 195, 556, 748, 782, 1047
Sau96I GGNCC 7 cut(s) 230, 413, 486, 591, 646, 852, 1126
SchI GAGTC 1 cut(s) 938
ScrFI CCNGG 6 cut(s) 97, 175, 240, 423, 753, 780
SduI GDGCHC 2 cut(s) 358, 430
SfaNI GCATC 6 cut(s) 76, 308, 1042, 1063, 1085, 1238
SfcI CTRYAG 1 cut(s) 330
Sfr303I CCGCGG 1 cut(s) 597
SgrBI CCGCGG 1 cut(s) 597
SinI GGWCC 3 cut(s) 413, 852, 1126
SmiMI CAYNNNNRTG 3 cut(s) 351, 609, 694
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 2 cut(s) 1056, 1224
SsiI CCGC 5 cut(s) 64, 255, 594, 596, 680
SspMI CTAG 2 cut(s) 282, 981
SstI GAGCTC 1 cut(s) 430
StyD4I CCNGG 6 cut(s) 95, 173, 238, 421, 751, 778
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 4 cut(s) 53, 292, 327, 459
TaiI ACGT 4 cut(s) 307, 412, 817, 1246
TaqI TCGA 2 cut(s) 775, 1050
TasI AATT 2 cut(s) 1056, 1224
TauI GCSGC 1 cut(s) 257
Tru1I TTAA 4 cut(s) 72, 113, 563, 954
Tru9I TTAA 4 cut(s) 72, 113, 563, 954
TscAI CASTG 3 cut(s) 232, 372, 982
TseI GCWGC 1 cut(s) 370
TspDTI ATGAA 6 cut(s) 257, 699, 926, 1050, 1110, 1176
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 3 cut(s) 232, 372, 982
Tth111I GACNNNGTC 1 cut(s) 411
Van91I CCANNNNNTGG 1 cut(s) 96
VpaK11BI GGWCC 3 cut(s) 413, 852, 1126
VspI ATTAAT 1 cut(s) 563
XagI CCTNNNNNAGG 1 cut(s) 420
XceI RCATGY 1 cut(s) 656
XmiI GTMKAC 1 cut(s) 775
XmnI GAANNNNTTC 2 cut(s) 729, 933
XspI CTAG 2 cut(s) 282, 981
Zsp2I ATGCAT 2 cut(s) 6, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.