AT5G15170

Tyrosyl-DNA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010258
Physical Location & Seq
Reverse (-)
4925850 .. 4929502
3653 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G15170.1

Sequence Viewer

Length: 1818 bp
ATGGCTCACTCTCAGGTTGCTTATTTGATTCCATTGAAAGCAGATTTAAAGGAAGACAATTCATCTCCAAGGATAACTTTATCCGAAGGTCCAAACATCATTGGCCGTGGCAACGTTTCAATCGTCGATAAGCGTCTCAGTCGCAAACACATCACCATTATCGTTTCCACTTCTGGCTCAGCTTCGTTGTCAGTGGACGGAACAAATCCGGTTGTTATTAGGTCTTCCGGTGACGGAGAGAGAAAGAAAGTGAAACCTAGTGAAGAGGTTTCAGTATGTAATGATGATCTTATTGAATTGATCCCTGGTCACCATTTCTTCAAGCTAGTTTTGCTCAATGGAAGAGCTGCTAAGAAAGCTCGAAAGGCTGAAGACGATGTAGAGGCCATTCGTCGTTTTTGTCCACCTAATGAGAAACTACCTTCAACATTTCGGCTTTTGAGTGTTGATGCATTGCCTGATTGGGCTAATACTTCTTGTGTTTCCATTAATGATGTCATTGAGGGAGACGTTGTTGCTGCGATCTTATCGAATTATATGGTTGATATAGACTGGTTGATGTCAGCTTGCCCAAAACTGGCCAACATTCCCCAGGTTATGGTTATTCATGGAGAAGGTGATGGTAGGCAAGAGTATATTCAGAGGAAGAAGCCAGCTAATTGGATTCTTCATAAGCCTCGGTTACCTATTTCATTCGGGACACACCATTCGAAGGCTATATTTCTTGTCTATCCTCGAGGGGTGAGAGTTGTAGTACACACCGCGAATCTGATCCATGTTGATTGGAATAACAAAAGCCAAGGTTTGTGGATGCAAGATTTTCCTTGGAAAGATGATGACAAAGATCCACCTAAAGGTTGTGGATTCGAAGGTGATCTCATTGATTACCTCAATGTTCTTAAGTGGCCTGAGTTTACTGCAAACTTACCTGGCCGTGGTAATGTCAAGATCAATGCAGCCTTCTTCAAAAAGTTTGATTATTCTGATGCTACGGTTCGACTAATTGCTTCAGTCCCTGGATACCACACTGGTTTTAACTTGAACAAATGGGGACACATGAAGCTACGGACTATTCTTCAAGAATGCATTTTCGATAGAGAGTTCCGCAGATCCCCGCTAATCTATCAGTTTTCTTCACTTGGTTCTTTAGACGAGAAGTGGTTGGCTGAGTTTGGAAATTCTTTGTCTTCTGGTATAACAGAGGACAAAACTCCTCTCGGTCCCGGGGATTCGTTGATAATATGGCCTACAGTAGAAGACGTCAGATGTTCTTTAGAGGGTTATGCTGCTGGCAATGCAATTCCGAGCCCATTGAAGAACGTGGAGAAACCATTCTTGAAAAAGTATTGGGCTAGATGGAAAGCGGATCATAGCGCTCGCGGTCGTGCCATGCCACATATAAAGACGTTCACACGTTACAACGACCAGAAGATTGCATGGTTCTTACTAACCTCATCGAATCTTAGCAAAGCGGCCTGGGGAGCACTTCAGAAGAACAATTCACAGTTGATGATTCGTTCCTACGAGTTGGGCGTCTTGTTTTTACCATCTCCAATAAAAACACAGGGTTGTGTATTTTCGTGCACTGAGAGCAACCCGAGTGTTATGAAGGCAAAACAAGAGACGAAAGACGAGGTAGAGAAGAGGAGTAAGCTAGTGACAATGACCTGGCAAGGAGACCGAGATTTGCCTGAGATAATCTCATTACCAGTTCCTTATCAACTACCTCCTAAGCCATACTCACCCGAAGATGTTCCATGGTCATGGGACCGAGGCTACTCGAAGAAAGATGTGTATGGACAAGTCTGGCCAAGATAG

Protein Analysis

605

Amino Acids

68.14

Weight (kDa)

8.99

Isoelectric Point (pI)

41.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyr-DNA_phospho PF06087 145 - 581 6.1e-100 Tyrosyl-DNA phosphodiesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1263
AbsI CCTCGAGG 1 cut(s) 735
AccB7I CCANNNNNTGG 1 cut(s) 598
AccII CGCG 2 cut(s) 764, 1380
AciI CCGC 6 cut(s) 762, 1105, 1115, 1364, 1380, 1472
AclI AACGTT 1 cut(s) 114
AclWI GGATC 5 cut(s) 295, 766, 839, 1104, 1374
AcoI YGGCCR 4 cut(s) 103, 579, 931, 1808
AcsI RAATTY 1 cut(s) 1177
AcuI CTGAAG 3 cut(s) 390, 993, 1472
AcyI GRCGYC 2 cut(s) 1260, 1533
AfaI GTAC 1 cut(s) 756
AfeI AGCGCT 1 cut(s) 1375
AfiI CCNNNNNNNGG 5 cut(s) 577, 598, 712, 854, 935
AflII CTTAAG 1 cut(s) 899
AflIII ACRYGT 1 cut(s) 1412
AjnI CCWGG 6 cut(s) 304, 591, 928, 1015, 1475, 1667
AluBI AGCT 8 cut(s) 182, 325, 347, 359, 566, 656, 1063, 1654
AluI AGCT 8 cut(s) 182, 325, 347, 359, 566, 656, 1063, 1654
Alw21I GWGCWC 2 cut(s) 1486, 1586
Alw26I GTCTC 4 cut(s) 140, 501, 1616, 1671
Alw44I GTGCAC 1 cut(s) 1582
AlwI GGATC 5 cut(s) 295, 766, 839, 1104, 1374
AlwNI CAGNNNCTG 1 cut(s) 1016
Ama87I CYCGRG 3 cut(s) 735, 1223, 1597
Aor51HI AGCGCT 1 cut(s) 1375
AoxI GGCC 8 cut(s) 103, 384, 579, 905, 931, 1244, 1473, 1808
ApaLI GTGCAC 1 cut(s) 1582
ApeKI GCWGC 4 cut(s) 347, 518, 956, 1286
ApoI RAATTY 1 cut(s) 1177
ArsI GACNNNNNNTTYG 2 cut(s) 691, 723
AseI ATTAAT 1 cut(s) 489
Asp700I GAANNNNTTC 2 cut(s) 1331, 1752
AspLEI GCGC 1 cut(s) 1376
AspS9I GGNCC 3 cut(s) 89, 1220, 1768
AsuC2I CCSGG 2 cut(s) 1224, 1225
AsuHPI GGTGA 7 cut(s) 145, 242, 302, 629, 754, 884, 1734
AsuII TTCGAA 2 cut(s) 710, 867
AvaI CYCGRG 3 cut(s) 735, 1223, 1597
AvaII GGWCC 3 cut(s) 89, 1220, 1768
BaeGI GKGCMC 1 cut(s) 1586
BalI TGGCCA 2 cut(s) 581, 1810
BanII GRGCYC 1 cut(s) 1310
BbsI GAAGAC 5 cut(s) 60, 216, 378, 1179, 1263
Bbv12I GWGCWC 2 cut(s) 1486, 1586
BbvI GCAGC 4 cut(s) 334, 505, 968, 1273
BccI CCATC 3 cut(s) 614, 1350, 1555
BceAI ACGGC 2 cut(s) 90, 918
BciT130I CCWGG 6 cut(s) 306, 593, 930, 1017, 1477, 1669
BciVI GTATCC 1 cut(s) 1013
BcnI CCSGG 2 cut(s) 1224, 1225
BcoDI GTCTC 4 cut(s) 140, 501, 1616, 1671
BfaI CTAG 4 cut(s) 258, 326, 1353, 1655
BfmI CTRYAG 1 cut(s) 1248
BfoI RGCGCY 1 cut(s) 1377
BfrI CTTAAG 1 cut(s) 899
BfuI GTATCC 1 cut(s) 1013
BisI GCNGC 5 cut(s) 348, 519, 957, 1287, 1473
BlpI GCTNAGC 1 cut(s) 178
BlsI GCNGC 5 cut(s) 349, 520, 958, 1288, 1474
Bme1390I CCNGG 8 cut(s) 306, 593, 930, 1017, 1224, 1225, 1477, 1669
Bme18I GGWCC 3 cut(s) 89, 1220, 1768
BmeT110I CYCGRG 3 cut(s) 735, 1223, 1597
BmgT120I GGNCC 3 cut(s) 89, 1220, 1768
BmiI GGNNCC 2 cut(s) 1222, 1769
BmrFI CCNGG 8 cut(s) 306, 593, 930, 1017, 1224, 1225, 1477, 1669
BmsI GCATC 3 cut(s) 439, 801, 976
BpiI GAAGAC 5 cut(s) 60, 216, 378, 1179, 1263
BplI GAGNNNNNCTC 2 cut(s) 1685, 1717
Bpu10I CCTNAGC 1 cut(s) 1731
Bpu1102I GCTNAGC 1 cut(s) 178
Bpu14I TTCGAA 2 cut(s) 710, 867
BpuMI CCSGG 2 cut(s) 1224, 1225
BsaHI GRCGYC 2 cut(s) 1260, 1533
BsaI GGTCTC 1 cut(s) 1671
BsaWI WCCGGW 2 cut(s) 208, 227
BsaXI ACNNNNNCTCC 2 cut(s) 498, 528
Bsc4I CCNNNNNNNGG 5 cut(s) 577, 598, 712, 854, 935
Bse1I ACTGG 4 cut(s) 557, 582, 1033, 1709
Bse3DI GCAATG 2 cut(s) 452, 1300
BseBI CCWGG 6 cut(s) 306, 593, 930, 1017, 1477, 1669
BseGI GGATG 1 cut(s) 816
BseLI CCNNNNNNNGG 5 cut(s) 577, 598, 712, 854, 935
BseMI GCAATG 2 cut(s) 452, 1300
BseMII CTCAG 7 cut(s) 26, 151, 192, 900, 1158, 1578, 1683
BseNI ACTGG 4 cut(s) 557, 582, 1033, 1709
BseRI GAGGAG 2 cut(s) 1203, 1660
BseSI GKGCMC 1 cut(s) 1586
BseXI GCAGC 4 cut(s) 334, 505, 968, 1273
Bsh1236I CGCG 2 cut(s) 764, 1380
Bsh1285I CGRYCG 1 cut(s) 1384
BshFI GGCC 8 cut(s) 105, 386, 581, 907, 933, 1246, 1475, 1810
BsiEI CGRYCG 1 cut(s) 1384
BsiHKAI GWGCWC 2 cut(s) 1486, 1586
BsiHKCI CYCGRG 3 cut(s) 735, 1223, 1597
BsiSI CCGG 3 cut(s) 209, 228, 1224
BslFI GGGAC 5 cut(s) 712, 998, 1065, 1206, 1781
BslI CCNNNNNNNGG 5 cut(s) 577, 598, 712, 854, 935
BsmAI GTCTC 4 cut(s) 140, 501, 1616, 1671
BsmBI CGTCTC 3 cut(s) 140, 501, 1616
BsmFI GGGAC 5 cut(s) 712, 998, 1065, 1206, 1781
BsmI GAATGC 1 cut(s) 1088
BsnI GGCC 8 cut(s) 105, 386, 581, 907, 933, 1246, 1475, 1810
Bso31I GGTCTC 1 cut(s) 1671
BsoBI CYCGRG 3 cut(s) 735, 1223, 1597
Bsp119I TTCGAA 2 cut(s) 710, 867
Bsp1286I GDGCHC 3 cut(s) 1310, 1486, 1586
Bsp143I GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
Bsp1720I GCTNAGC 1 cut(s) 178
Bsp19I CCATGG 1 cut(s) 1757
BspACI CCGC 6 cut(s) 762, 1105, 1115, 1364, 1380, 1472
BspANI GGCC 8 cut(s) 105, 386, 581, 907, 933, 1246, 1475, 1810
BspCNI CTCAG 7 cut(s) 25, 150, 191, 901, 1159, 1579, 1684
BspFNI CGCG 2 cut(s) 764, 1380
BspLI GGNNCC 2 cut(s) 1222, 1769
BspPI GGATC 5 cut(s) 295, 766, 839, 1104, 1374
BspQI GCTCTTC 1 cut(s) 337
BspT104I TTCGAA 2 cut(s) 710, 867
BspTI CTTAAG 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 1671
BsrDI GCAATG 2 cut(s) 452, 1300
BsrI ACTGG 4 cut(s) 557, 582, 1033, 1709
BssMI GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
BssNI GRCGYC 2 cut(s) 1260, 1533
BssT1I CCWWGG 4 cut(s) 68, 799, 824, 1757
Bst2UI CCWGG 6 cut(s) 306, 593, 930, 1017, 1477, 1669
Bst4CI ACNGT 3 cut(s) 994, 1252, 1506
Bst6I CTCTTC 3 cut(s) 258, 337, 1637
BstACI GRCGYC 2 cut(s) 1260, 1533
BstAFI CTTAAG 1 cut(s) 899
BstBI TTCGAA 2 cut(s) 710, 867
BstC8I GCNNGC 4 cut(s) 568, 654, 1291, 1378
BstDSI CCRYGG 3 cut(s) 106, 934, 1757
BstEII GGTNACC 2 cut(s) 308, 681
BstF5I GGATG 1 cut(s) 816
BstFNI CGCG 2 cut(s) 764, 1380
BstH2I RGCGCY 1 cut(s) 1377
BstHHI GCGC 1 cut(s) 1376
BstKTI GATC 9 cut(s) 289, 303, 525, 774, 847, 877, 951, 1112, 1369
BstMAI GTCTC 4 cut(s) 140, 501, 1616, 1671
BstMBI GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
BstMCI CGRYCG 1 cut(s) 1384
BstMWI GCNNNNNNNGC 6 cut(s) 331, 356, 365, 1295, 1481, 1590
BstNI CCWGG 6 cut(s) 306, 593, 930, 1017, 1477, 1669
BstPI GGTNACC 2 cut(s) 308, 681
BstSCI CCNGG 8 cut(s) 304, 591, 928, 1015, 1222, 1223, 1475, 1667
BstSFI CTRYAG 1 cut(s) 1248
BstSLI GKGCMC 1 cut(s) 1586
BstUI CGCG 2 cut(s) 764, 1380
BstV1I GCAGC 4 cut(s) 334, 505, 968, 1273
BstV2I GAAGAC 5 cut(s) 60, 216, 378, 1179, 1263
BstX2I RGATCY 2 cut(s) 844, 1109
BstXI CCANNNNNNTGG 2 cut(s) 660, 1764
BstYI RGATCY 2 cut(s) 844, 1109
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 8 cut(s) 105, 386, 581, 907, 933, 1246, 1475, 1810
BtgI CCRYGG 3 cut(s) 106, 934, 1757
BtsCI GGATG 1 cut(s) 816
BtsIMutI CAGTG 3 cut(s) 198, 1026, 1584
Cac8I GCNNGC 4 cut(s) 568, 654, 1291, 1378
CaiI CAGNNNCTG 1 cut(s) 1016
CfoI GCGC 1 cut(s) 1376
Cfr13I GGNCC 3 cut(s) 89, 1220, 1768
Cfr9I CCCGGG 1 cut(s) 1223
CseI GACGC 2 cut(s) 122, 1522
Csp6I GTAC 1 cut(s) 755
CspCI CAANNNNNGTGG 2 cut(s) 788, 823
CviAII CATG 7 cut(s) 608, 776, 1057, 1390, 1437, 1758, 1764
CviQI GTAC 1 cut(s) 755
DpnI GATC 9 cut(s) 288, 302, 524, 773, 846, 876, 950, 1111, 1368
DpnII GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
DraI TTTAAA 1 cut(s) 48
EaeI YGGCCR 4 cut(s) 103, 579, 931, 1808
Eam1104I CTCTTC 3 cut(s) 258, 337, 1637
EarI CTCTTC 3 cut(s) 258, 337, 1637
Eco130I CCWWGG 4 cut(s) 68, 799, 824, 1757
Eco24I GRGCYC 1 cut(s) 1310
Eco31I GGTCTC 1 cut(s) 1671
Eco47I GGWCC 3 cut(s) 89, 1220, 1768
Eco47III AGCGCT 1 cut(s) 1375
Eco57I CTGAAG 3 cut(s) 390, 993, 1472
Eco88I CYCGRG 3 cut(s) 735, 1223, 1597
Eco91I GGTNACC 2 cut(s) 308, 681
EcoO65I GGTNACC 2 cut(s) 308, 681
EcoRII CCWGG 6 cut(s) 304, 591, 928, 1015, 1475, 1667
EcoT14I CCWWGG 4 cut(s) 68, 799, 824, 1757
EcoT22I ATGCAT 2 cut(s) 454, 1088
EcoT38I GRGCYC 1 cut(s) 1310
ErhI CCWWGG 4 cut(s) 68, 799, 824, 1757
Esp3I CGTCTC 3 cut(s) 140, 501, 1616
FaeI CATG 7 cut(s) 611, 779, 1060, 1393, 1440, 1761, 1767
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FaqI GGGAC 5 cut(s) 712, 998, 1065, 1206, 1781
FatI CATG 7 cut(s) 607, 775, 1056, 1389, 1436, 1757, 1763
FauI CCCGC 1 cut(s) 1122
Fnu4HI GCNGC 5 cut(s) 348, 519, 957, 1287, 1473
FokI GGATG 1 cut(s) 823
FriOI GRGCYC 1 cut(s) 1310
Fsp4HI GCNGC 5 cut(s) 348, 519, 957, 1287, 1473
FspBI CTAG 4 cut(s) 258, 326, 1353, 1655
GlaI GCGC 1 cut(s) 1375
GluI GCNGC 5 cut(s) 348, 519, 957, 1287, 1473
HaeII RGCGCY 1 cut(s) 1377
HaeIII GGCC 8 cut(s) 105, 386, 581, 907, 933, 1246, 1475, 1810
HapII CCGG 3 cut(s) 209, 228, 1224
HgaI GACGC 2 cut(s) 122, 1522
HhaI GCGC 1 cut(s) 1376
Hin1I GRCGYC 2 cut(s) 1260, 1533
Hin1II CATG 7 cut(s) 611, 779, 1060, 1393, 1440, 1761, 1767
Hin6I GCGC 1 cut(s) 1374
HinP1I GCGC 1 cut(s) 1374
HinfI GANTC 7 cut(s) 28, 664, 766, 864, 1229, 1459, 1513
HpaII CCGG 3 cut(s) 209, 228, 1224
HphI GGTGA 7 cut(s) 145, 242, 302, 629, 754, 884, 1734
Hpy166II GTNNAC 6 cut(s) 196, 404, 757, 915, 1410, 1584
Hpy188I TCNGA 7 cut(s) 85, 642, 771, 985, 1265, 1305, 1491
Hpy188III TCNNGA 4 cut(s) 697, 946, 1079, 1336
Hpy8I GTNNAC 6 cut(s) 196, 404, 757, 915, 1410, 1584
Hpy99I CGWCG 2 cut(s) 128, 396
HpyAV CCTTC 7 cut(s) 80, 432, 608, 706, 863, 970, 1603
HpyCH4III ACNGT 3 cut(s) 994, 1252, 1506
HpyCH4IV ACGT 6 cut(s) 114, 510, 1260, 1320, 1406, 1414
HpyCH4V TGCA 8 cut(s) 452, 814, 920, 956, 1086, 1298, 1436, 1584
HpyF10VI GCNNNNNNNGC 6 cut(s) 331, 356, 365, 1295, 1481, 1590
HpySE526I ACGT 6 cut(s) 114, 510, 1260, 1320, 1406, 1414
Hsp92I GRCGYC 2 cut(s) 1260, 1533
Hsp92II CATG 7 cut(s) 611, 779, 1060, 1393, 1440, 1761, 1767
HspAI GCGC 1 cut(s) 1374
Kzo9I GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
LguI GCTCTTC 1 cut(s) 337
LmnI GCTCC 1 cut(s) 1481
Lsp1109I GCAGC 4 cut(s) 334, 505, 968, 1273
LweI GCATC 3 cut(s) 439, 801, 976
MaeI CTAG 4 cut(s) 258, 326, 1353, 1655
MaeII ACGT 6 cut(s) 114, 510, 1260, 1320, 1406, 1414
MaeIII GTNAC 5 cut(s) 230, 308, 681, 1415, 1657
MalI GATC 9 cut(s) 288, 302, 524, 773, 846, 876, 950, 1111, 1368
MboI GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
MflI RGATCY 2 cut(s) 844, 1109
MhlI GDGCHC 3 cut(s) 1310, 1486, 1586
MlsI TGGCCA 2 cut(s) 581, 1810
MluCI AATT 8 cut(s) 58, 296, 532, 658, 1002, 1177, 1299, 1498
MluNI TGGCCA 2 cut(s) 581, 1810
Mox20I TGGCCA 2 cut(s) 581, 1810
Mph1103I ATGCAT 2 cut(s) 454, 1088
MroXI GAANNNNTTC 2 cut(s) 1331, 1752
MscI TGGCCA 2 cut(s) 581, 1810
MseI TTAA 4 cut(s) 47, 489, 900, 1035
MslI CAYNNNNRTG 1 cut(s) 1762
Msp20I TGGCCA 2 cut(s) 581, 1810
MspCI CTTAAG 1 cut(s) 899
MspI CCGG 3 cut(s) 209, 228, 1224
MspR9I CCNGG 8 cut(s) 306, 593, 930, 1017, 1224, 1225, 1477, 1669
Mva1269I GAATGC 1 cut(s) 1088
MvaI CCWGG 6 cut(s) 306, 593, 930, 1017, 1477, 1669
MvnI CGCG 2 cut(s) 764, 1380
MwoI GCNNNNNNNGC 6 cut(s) 331, 356, 365, 1295, 1481, 1590
NciI CCSGG 2 cut(s) 1224, 1225
NcoI CCATGG 1 cut(s) 1757
NdeII GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
NlaIII CATG 7 cut(s) 611, 779, 1060, 1393, 1440, 1761, 1767
NlaIV GGNNCC 2 cut(s) 1222, 1769
NmuCI GTSAC 3 cut(s) 230, 308, 1657
NsiI ATGCAT 2 cut(s) 454, 1088
NspV TTCGAA 2 cut(s) 710, 867
PaeR7I CTCGAG 1 cut(s) 735
PciSI GCTCTTC 1 cut(s) 337
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 1530
PctI GAATGC 1 cut(s) 1088
PdmI GAANNNNTTC 2 cut(s) 1331, 1752
PfeI GAWTC 7 cut(s) 28, 664, 766, 864, 1229, 1459, 1513
PflMI CCANNNNNTGG 1 cut(s) 598
PkrI GCNGC 5 cut(s) 349, 520, 958, 1288, 1474
PshBI ATTAAT 1 cut(s) 489
Psp1406I AACGTT 1 cut(s) 114
Psp6I CCWGG 6 cut(s) 304, 591, 928, 1015, 1475, 1667
PspEI GGTNACC 2 cut(s) 308, 681
PspGI CCWGG 6 cut(s) 304, 591, 928, 1015, 1475, 1667
PspN4I GGNNCC 2 cut(s) 1222, 1769
PspPI GGNCC 3 cut(s) 89, 1220, 1768
PspXI VCTCGAGB 1 cut(s) 735
PstNI CAGNNNCTG 1 cut(s) 1016
PsuI RGATCY 2 cut(s) 844, 1109
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
RseI CAYNNNNRTG 1 cut(s) 1762
SapI GCTCTTC 1 cut(s) 337
SaqAI TTAA 4 cut(s) 47, 489, 900, 1035
SatI GCNGC 5 cut(s) 348, 519, 957, 1287, 1473
Sau3AI GATC 9 cut(s) 286, 300, 522, 771, 844, 874, 948, 1109, 1366
Sau96I GGNCC 3 cut(s) 89, 1220, 1768
ScrFI CCNGG 8 cut(s) 306, 593, 930, 1017, 1224, 1225, 1477, 1669
SduI GDGCHC 3 cut(s) 1310, 1486, 1586
SfaNI GCATC 3 cut(s) 439, 801, 976
SfcI CTRYAG 1 cut(s) 1248
Sfr274I CTCGAG 1 cut(s) 735
SfuI TTCGAA 2 cut(s) 710, 867
SinI GGWCC 3 cut(s) 89, 1220, 1768
SlaI CTCGAG 1 cut(s) 735
SmaI CCCGGG 1 cut(s) 1225
SmiMI CAYNNNNRTG 1 cut(s) 1762
SmlI CTYRAG 2 cut(s) 735, 899
SmoI CTYRAG 2 cut(s) 735, 899
Sse9I AATT 8 cut(s) 58, 296, 532, 658, 1002, 1177, 1299, 1498
SsiI CCGC 6 cut(s) 762, 1105, 1115, 1364, 1380, 1472
SspMI CTAG 4 cut(s) 258, 326, 1353, 1655
StyD4I CCNGG 8 cut(s) 304, 591, 928, 1015, 1222, 1223, 1475, 1667
StyI CCWWGG 4 cut(s) 68, 799, 824, 1757
TaaI ACNGT 3 cut(s) 994, 1252, 1506
TaiI ACGT 6 cut(s) 117, 513, 1263, 1323, 1409, 1417
TaqII GACCGA 3 cut(s) 1208, 1695, 1785
TasI AATT 8 cut(s) 58, 296, 532, 658, 1002, 1177, 1299, 1498
TatI WGTACW 1 cut(s) 754
TauI GCSGC 1 cut(s) 1475
TfiI GAWTC 7 cut(s) 28, 664, 766, 864, 1229, 1459, 1513
Tru1I TTAA 4 cut(s) 47, 489, 900, 1035
Tru9I TTAA 4 cut(s) 47, 489, 900, 1035
TscAI CASTG 3 cut(s) 198, 1033, 1591
TseFI GTSAC 3 cut(s) 230, 308, 1657
TseI GCWGC 4 cut(s) 347, 518, 956, 1286
Tsp45I GTSAC 3 cut(s) 230, 308, 1657
TspDTI ATGAA 6 cut(s) 51, 596, 659, 681, 1073, 1622
TspGWI ACGGA 3 cut(s) 213, 249, 1081
TspMI CCCGGG 1 cut(s) 1223
TspRI CASTG 3 cut(s) 198, 1033, 1591
Van91I CCANNNNNTGG 1 cut(s) 598
Vha464I CTTAAG 1 cut(s) 899
VneI GTGCAC 1 cut(s) 1582
VpaK11BI GGWCC 3 cut(s) 89, 1220, 1768
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 1 cut(s) 1177
XhoI CTCGAG 1 cut(s) 735
XmaI CCCGGG 1 cut(s) 1223
XmnI GAANNNNTTC 2 cut(s) 1331, 1752
XspI CTAG 4 cut(s) 258, 326, 1353, 1655
ZraI GACGTC 1 cut(s) 1261
Zsp2I ATGCAT 2 cut(s) 454, 1088
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.