Rw5G003300

Tyrosyl-DNA phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010258
Physical Location & Seq
Reverse (-)
3024445 .. 3030701
6257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G003300.1

Sequence Viewer

Length: 1995 bp
ATGGATCACGGAATGGGCGAAGCTGTAACCCAAACCCAAGTAGGACACTTGGTTCCCTTAAGCAACCACGTCCAAGAAGACGCTTCAATCCCCAAAATACCCCTCTCCCGGGGCCGGAATTCCATCGGCCGCAACTCCGCCCCTTCCCTCACCGACAAGCGTCTCAGCCGCAACCACCTCTCCCTCACAGCCGCCGCGGCCGACGCCTCCTCCGCCGATTTGGTCGTGGAGGGAAGCAATCCGGTTGTGGTAACTAGCGGAAATGATAGGAAGAAACTGTATCATAAAGAAAGTATGACGATTCGAGACGGTGATATTATAGAGCTGATTCCAGGGCAGTATATTTTTCAGTATAGGAAATTGTGTGGTGACAGTTATGAGAAGTGTTCAAATGGTGAAGAGAAGGCTGAGGAGTCTAGTCGAAAGCGGTCACATCAGTTAGAATGTGGAGAGAATGGTTCGAATTCGAAGCGTTTTAAGGGTGAGGTGGAAGGGAAGAGTGATGATGGTGGTGGTCGTGTGGAGGCGATACGGCATTTTCGTGTTCCTAATGAAAAGTTGCCACATACGTTTCGGCTTTTGAATGTGCAAGGACTGCCCCCGTGGGCGAATACCTCGTGTGTTTCTATAAAGGATGTGATTCAGGGAGACATCCTTTTTGCTGTCCTTTCCAATTACATGGTAGACCTTGATTGGCTGATACCTGCTTGTCCTACAATTGCAAAAATCCCACATGTATTGGTTGTTCATGGTGAGGGTGATGGCACACTGGATTATATGAAGAGAAAGAAACCTGCAAATTGGATTCTACACAAACCACCATTGCCTATTTCATTTGGGACACACCACTCAAAGGCCATGCTTCTTGTTTATCCTCGAGGAGTGAGAATAATAGTACATACAGCAAACTTAATATTTGTTGATTGGAACAACAAAAGTCAAGGTTTGTGGATGCAAGATTTTCCCTGGAAAGATCAAAGTAATCCAAGCAAAGGTTGTGGATTTGAACATGACTTAATTGACTATCTCAGTGCACTGAAGTGGCCTGAGTTTTCTGTTAATTTGCCTACCCTGGGAACCTTCAATATAAATCCAGGATTTTTCAAGAAATTCGATTACAGCAGTGCAGCAGTGAGGCTAATTGCATCTGTCCCTGGATATCATACCGGTAATAACATGAAAAAGTGGGGACATATGAAACTACGTACTGTTCTCCAGGAGTGTACTTTTGATAAAGAGTTTCGGAAATCTCCTCTTGCTTATCAGTTCTCCTCCCTAGGTTCTTTGGATGAGAAATGGATGGCTGAGCTTGCACATTCAATGTCATCAGGTTTTTCGGATGATAAAACTCCTCTCGGTCCTGGGGAGCCACGTATAATATGGCCCACAGTAGAAGATGTTAGATGCTCTATAGAGGGTTATGCTGCTGGAAATGCTGTTCCAAGTCCAGCAAAGAATGTGGATAAAGATTTCTTGAAGAAGTACTGGGCAAGATGGAAGGCAAGCCACACTGGCCGCTGTCGTGCAATGCCACATATAAAGACATTTACTCGATACAGCGGTCAGAAACTTGCTTGGTTTTTGCTAACATCAGCGAATCTTAGCAAAGCCGCATGGGGAGCTCTCCAGAAGAATAATTCTCAACTCATGATCCGTTCTTATGAGCTTGGGGTGCTGTTTCTACCATATAAAACACGAAGTCCTGGATTTTCTTGTACCAGTAATGCGGTTCCATCTGAGGATAAATCTGAAGAGTCAGATAATTCGGGATCAAGGAGAAATAAACTAGTGACTCTAAACTGGCAAGGGAACAGGAACACAGATTCATCATCTGAAGTAATCACATTGCCTGTGCCCTATGAACTCCCTCCTCTACCATACACCACCCAAGATGTTCCTTGGTCATGGGACCGTCGATACACCAAGAAAGATGTTTATGGTCAAGTTTGGCCGCGGCAAGTTCAGCTATATGCAGCAGCTCTTCAAGATTCTTGA

Protein Analysis

664

Amino Acids

74.44

Weight (kDa)

9.04

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyr-DNA_phospho PF06087 191 - 628 8.3e-102 Tyrosyl-DNA phosphodiesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 876
Acc36I ACCTGC 2 cut(s) 712, 802
AccI GTMKAC 1 cut(s) 684
AccII CGCG 2 cut(s) 197, 1954
AclWI GGATC 3 cut(s) 12, 1645, 1777
AcoI YGGCCR 4 cut(s) 127, 198, 1513, 1949
AcsI RAATTY 3 cut(s) 118, 463, 1109
AcuI CTGAAG 3 cut(s) 1058, 1770, 1854
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 5 cut(s) 897, 1207, 1225, 1484, 1717
AfiI CCNNNNNNNGG 6 cut(s) 108, 109, 114, 853, 992, 1073
AflII CTTAAG 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 733
AgeI ACCGGT 1 cut(s) 1166
AgsI TTSAA 9 cut(s) 87, 390, 583, 1007, 1084, 1105, 1320, 1477, 1985
AhlI ACTAGT 1 cut(s) 1786
AjiI CACGTC 1 cut(s) 70
AjnI CCWGG 8 cut(s) 331, 965, 1071, 1093, 1153, 1215, 1360, 1702
AleI CACNNNNGTG 1 cut(s) 1039
AloI GAACNNNNNNTCC 4 cut(s) 36, 68, 1422, 1454
AluBI AGCT 7 cut(s) 23, 325, 1309, 1622, 1666, 1966, 1979
AluI AGCT 7 cut(s) 23, 325, 1309, 1622, 1666, 1966, 1979
Alw21I GWGCWC 2 cut(s) 1036, 1624
Alw26I GTCTC 3 cut(s) 167, 300, 642
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 3 cut(s) 12, 1645, 1777
Ama87I CYCGRG 2 cut(s) 108, 876
AoxI GGCC 8 cut(s) 112, 127, 198, 855, 1043, 1382, 1513, 1949
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 4 cut(s) 1127, 1424, 1973, 1976
ApoI RAATTY 3 cut(s) 118, 463, 1109
ArsI GACNNNNNNTTYG 2 cut(s) 641, 673
AsiGI ACCGGT 1 cut(s) 1166
AspA2I CCTAGG 1 cut(s) 1276
AspS9I GGNCC 4 cut(s) 112, 1358, 1383, 1909
AsuC2I CCSGG 2 cut(s) 109, 110
AsuHPI GGTGA 7 cut(s) 142, 323, 380, 407, 494, 764, 770
AsuII TTCGAA 2 cut(s) 461, 467
AvaI CYCGRG 2 cut(s) 108, 876
AvaII GGWCC 2 cut(s) 1358, 1909
AvrII CCTAGG 1 cut(s) 1276
BaeGI GKGCMC 2 cut(s) 1036, 1857
BaeI ACNNNNGTAYC 2 cut(s) 1546, 1579
BanII GRGCYC 1 cut(s) 1624
BauI CACGAG 1 cut(s) 616
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 1036, 1624
BbvCI CCTCAGC 1 cut(s) 408
BbvI GCAGC 4 cut(s) 1139, 1411, 1985, 1988
BccI CCATC 6 cut(s) 131, 500, 755, 1294, 1488, 1741
BceAI ACGGC 1 cut(s) 548
BciT130I CCWGG 8 cut(s) 333, 967, 1073, 1095, 1155, 1217, 1362, 1704
BcnI CCSGG 2 cut(s) 109, 110
BcoDI GTCTC 3 cut(s) 167, 300, 642
BcuI ACTAGT 1 cut(s) 1786
BfaI CTAG 4 cut(s) 255, 417, 1277, 1787
BfmI CTRYAG 1 cut(s) 1410
BfrI CTTAAG 1 cut(s) 58
BfuAI ACCTGC 2 cut(s) 712, 802
BglI GCCNNNNNGGC 2 cut(s) 197, 1512
BlnI CCTAGG 1 cut(s) 1276
BlpI GCTNAGC 1 cut(s) 1305
BmcAI AGTACT 1 cut(s) 1484
Bme18I GGWCC 2 cut(s) 1358, 1909
BmeT110I CYCGRG 2 cut(s) 108, 876
BmgBI CACGTC 1 cut(s) 70
BmgT120I GGNCC 4 cut(s) 112, 1358, 1383, 1909
BmiI GGNNCC 6 cut(s) 54, 113, 1078, 1368, 1731, 1910
BmrI ACTGGG 1 cut(s) 1495
BmsI GCATC 3 cut(s) 942, 1154, 1394
BmuI ACTGGG 1 cut(s) 1495
BoxI GACNNNNGTC 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 2 cut(s) 1199, 1610
Bpu10I CCTNAGC 1 cut(s) 408
Bpu1102I GCTNAGC 1 cut(s) 1305
Bpu14I TTCGAA 2 cut(s) 461, 467
BpuMI CCSGG 2 cut(s) 109, 110
BsaAI YACGTR 2 cut(s) 1205, 1373
BsaHI GRCGYC 1 cut(s) 204
BsaWI WCCGGW 2 cut(s) 241, 1166
BsaXI ACNNNNNCTCC 4 cut(s) 164, 194, 194, 224
Bsc4I CCNNNNNNNGG 6 cut(s) 108, 109, 114, 853, 992, 1073
Bse118I RCCGGY 1 cut(s) 1166
Bse1I ACTGG 5 cut(s) 774, 1490, 1516, 1719, 1805
Bse3DI GCAATG 3 cut(s) 821, 1533, 1844
BseBI CCWGG 8 cut(s) 333, 967, 1073, 1095, 1155, 1217, 1362, 1704
BseGI GGATG 6 cut(s) 640, 651, 957, 1294, 1305, 1345
BseLI CCNNNNNNNGG 6 cut(s) 108, 109, 114, 853, 992, 1073
BseMI GCAATG 3 cut(s) 821, 1533, 1844
BseMII CTCAG 6 cut(s) 178, 399, 1038, 1042, 1296, 1728
BseNI ACTGG 5 cut(s) 774, 1490, 1516, 1719, 1805
BseRI GAGGAG 7 cut(s) 199, 425, 894, 1242, 1261, 1341, 1860
BseSI GKGCMC 2 cut(s) 1036, 1857
BseX3I CGGCCG 2 cut(s) 127, 198
BseXI GCAGC 4 cut(s) 1139, 1411, 1985, 1988
BsgI GTGCAG 1 cut(s) 1146
Bsh1236I CGCG 2 cut(s) 197, 1954
Bsh1285I CGRYCG 2 cut(s) 130, 201
BshFI GGCC 8 cut(s) 114, 129, 200, 857, 1045, 1384, 1515, 1951
BshTI ACCGGT 1 cut(s) 1166
BsiEI CGRYCG 2 cut(s) 130, 201
BsiHKAI GWGCWC 2 cut(s) 1036, 1624
BsiHKCI CYCGRG 2 cut(s) 108, 876
BsiSI CCGG 4 cut(s) 109, 115, 242, 1167
BslFI GGGAC 4 cut(s) 853, 1136, 1203, 1922
BslI CCNNNNNNNGG 6 cut(s) 108, 109, 114, 853, 992, 1073
BsmAI GTCTC 3 cut(s) 167, 300, 642
BsmBI CGTCTC 2 cut(s) 167, 300
BsmFI GGGAC 4 cut(s) 853, 1136, 1203, 1922
BsnI GGCC 8 cut(s) 114, 129, 200, 857, 1045, 1384, 1515, 1951
BsoBI CYCGRG 2 cut(s) 108, 876
Bsp119I TTCGAA 2 cut(s) 461, 467
Bsp1286I GDGCHC 3 cut(s) 1036, 1624, 1857
Bsp143I GATC 4 cut(s) 4, 973, 1650, 1769
Bsp1720I GCTNAGC 1 cut(s) 1305
BspANI GGCC 8 cut(s) 114, 129, 200, 857, 1045, 1384, 1515, 1951
BspCNI CTCAG 6 cut(s) 177, 400, 1039, 1041, 1297, 1729
BspFNI CGCG 2 cut(s) 197, 1954
BspHI TCATGA 1 cut(s) 1647
BspLI GGNNCC 6 cut(s) 54, 113, 1078, 1368, 1731, 1910
BspMI ACCTGC 2 cut(s) 712, 802
BspPI GGATC 3 cut(s) 12, 1645, 1777
BspQI GCTCTTC 1 cut(s) 1986
BspT104I TTCGAA 2 cut(s) 461, 467
BspTI CTTAAG 1 cut(s) 58
BsrDI GCAATG 3 cut(s) 821, 1533, 1844
BsrFI RCCGGY 1 cut(s) 1166
BsrI ACTGG 5 cut(s) 774, 1490, 1516, 1719, 1805
BssAI RCCGGY 1 cut(s) 1166
BssMI GATC 4 cut(s) 4, 973, 1650, 1769
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 616
BssT1I CCWWGG 2 cut(s) 1276, 1898
Bst2BI CACGAG 1 cut(s) 616
Bst2UI CCWGG 8 cut(s) 333, 967, 1073, 1095, 1155, 1217, 1362, 1704
Bst4CI ACNGT 6 cut(s) 279, 311, 374, 1210, 1390, 1913
Bst6I CTCTTC 5 cut(s) 393, 491, 776, 1746, 1986
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 58
BstAPI GCANNNNNTGC 1 cut(s) 595
BstBAI YACGTR 2 cut(s) 1205, 1373
BstBI TTCGAA 2 cut(s) 461, 467
BstC8I GCNNGC 2 cut(s) 1311, 1504
BstDEI CTNAG 7 cut(s) 164, 408, 1028, 1047, 1305, 1601, 1737
BstDSI CCRYGG 3 cut(s) 195, 602, 1952
BstF5I GGATG 6 cut(s) 640, 651, 957, 1294, 1305, 1345
BstFNI CGCG 2 cut(s) 197, 1954
BstKTI GATC 4 cut(s) 7, 976, 1653, 1772
BstMAI GTCTC 3 cut(s) 167, 300, 642
BstMBI GATC 4 cut(s) 4, 973, 1650, 1769
BstMCI CGRYCG 2 cut(s) 130, 201
BstNI CCWGG 8 cut(s) 333, 967, 1073, 1095, 1155, 1217, 1362, 1704
BstNSI RCATGY 1 cut(s) 737
BstPAI GACNNNNGTC 1 cut(s) 159
BstSFI CTRYAG 1 cut(s) 1410
BstSLI GKGCMC 2 cut(s) 1036, 1857
BstSNI TACGTA 1 cut(s) 1205
BstUI CGCG 2 cut(s) 197, 1954
BstV1I GCAGC 4 cut(s) 1139, 1411, 1985, 1988
BstV2I GAAGAC 1 cut(s) 84
BstXI CCANNNNNNTGG 1 cut(s) 679
BstZI CGGCCG 2 cut(s) 127, 198
BsuRI GGCC 8 cut(s) 114, 129, 200, 857, 1045, 1384, 1515, 1951
BtgI CCRYGG 3 cut(s) 195, 602, 1952
BtrI CACGTC 1 cut(s) 70
BtsCI GGATG 6 cut(s) 640, 651, 957, 1294, 1305, 1345
BtsI GCAGTG 2 cut(s) 1129, 1137
BtsIMutI CAGTG 6 cut(s) 767, 1034, 1036, 1129, 1137, 1509
BveI ACCTGC 2 cut(s) 712, 802
Cac8I GCNNGC 2 cut(s) 1311, 1504
CciI TCATGA 1 cut(s) 1647
Cfr10I RCCGGY 1 cut(s) 1166
Cfr13I GGNCC 4 cut(s) 112, 1358, 1383, 1909
Cfr42I CCGCGG 2 cut(s) 198, 1955
Cfr9I CCCGGG 1 cut(s) 108
CseI GACGC 3 cut(s) 89, 149, 212
Csp6I GTAC 5 cut(s) 896, 1206, 1224, 1483, 1716
CspAI ACCGGT 1 cut(s) 1166
CspCI CAANNNNNGTGG 8 cut(s) 720, 755, 929, 964, 979, 1014, 1440, 1475
CviAII CATG 9 cut(s) 679, 734, 749, 859, 1010, 1177, 1614, 1648, 1905
CviQI GTAC 5 cut(s) 896, 1206, 1224, 1483, 1716
DdeI CTNAG 7 cut(s) 164, 408, 1028, 1047, 1305, 1601, 1737
DpnI GATC 4 cut(s) 6, 975, 1652, 1771
DpnII GATC 4 cut(s) 4, 973, 1650, 1769
EaeI YGGCCR 4 cut(s) 127, 198, 1513, 1949
EagI CGGCCG 2 cut(s) 127, 198
Eam1104I CTCTTC 5 cut(s) 393, 491, 776, 1746, 1986
EarI CTCTTC 5 cut(s) 393, 491, 776, 1746, 1986
EciI GGCGGA 2 cut(s) 127, 202
Ecl136II GAGCTC 1 cut(s) 1622
EclXI CGGCCG 2 cut(s) 127, 198
Eco105I TACGTA 1 cut(s) 1205
Eco130I CCWWGG 2 cut(s) 1276, 1898
Eco24I GRGCYC 1 cut(s) 1624
Eco32I GATATC 1 cut(s) 1160
Eco47I GGWCC 2 cut(s) 1358, 1909
Eco52I CGGCCG 2 cut(s) 127, 198
Eco53kI GAGCTC 1 cut(s) 1622
Eco57I CTGAAG 3 cut(s) 1058, 1770, 1854
Eco88I CYCGRG 2 cut(s) 108, 876
EcoICRI GAGCTC 1 cut(s) 1622
EcoRI GAATTC 2 cut(s) 118, 463
EcoRII CCWGG 8 cut(s) 331, 965, 1071, 1093, 1153, 1215, 1360, 1702
EcoRV GATATC 1 cut(s) 1160
EcoT14I CCWWGG 2 cut(s) 1276, 1898
EcoT38I GRGCYC 1 cut(s) 1624
ErhI CCWWGG 2 cut(s) 1276, 1898
Esp3I CGTCTC 2 cut(s) 167, 300
FaeI CATG 9 cut(s) 682, 737, 752, 862, 1013, 1180, 1617, 1651, 1908
FaqI GGGAC 4 cut(s) 853, 1136, 1203, 1922
FatI CATG 9 cut(s) 678, 733, 748, 858, 1009, 1176, 1613, 1647, 1904
FauNDI CATATG 1 cut(s) 1194
FblI GTMKAC 1 cut(s) 684
FokI GGATG 6 cut(s) 638, 647, 964, 1301, 1312, 1352
FriOI GRGCYC 1 cut(s) 1624
FspBI CTAG 4 cut(s) 255, 417, 1277, 1787
GsuI CTGGAG 2 cut(s) 1199, 1610
HaeIII GGCC 8 cut(s) 114, 129, 200, 857, 1045, 1384, 1515, 1951
HapII CCGG 4 cut(s) 109, 115, 242, 1167
HgaI GACGC 3 cut(s) 89, 149, 212
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 9 cut(s) 682, 737, 752, 862, 1013, 1180, 1617, 1651, 1908
HpaII CCGG 4 cut(s) 109, 115, 242, 1167
HphI GGTGA 7 cut(s) 142, 323, 380, 407, 494, 764, 770
Hpy166II GTNNAC 3 cut(s) 685, 1034, 1224
Hpy188I TCNGA 7 cut(s) 1245, 1339, 1566, 1738, 1750, 1759, 1834
Hpy188III TCNNGA 8 cut(s) 305, 1105, 1474, 1627, 1648, 1767, 1985, 1992
Hpy8I GTNNAC 3 cut(s) 685, 1034, 1224
Hpy99I CGWCG 2 cut(s) 206, 1917
HpyAV CCTTC 5 cut(s) 153, 397, 485, 1090, 1492
HpyCH4III ACNGT 6 cut(s) 279, 311, 374, 1210, 1390, 1913
HpyCH4IV ACGT 4 cut(s) 69, 569, 1204, 1372
HpyF3I CTNAG 7 cut(s) 164, 408, 1028, 1047, 1305, 1601, 1737
HpySE526I ACGT 4 cut(s) 69, 569, 1204, 1372
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 9 cut(s) 682, 737, 752, 862, 1013, 1180, 1617, 1651, 1908
KspI CCGCGG 2 cut(s) 198, 1955
Kzo9I GATC 4 cut(s) 4, 973, 1650, 1769
LguI GCTCTTC 1 cut(s) 1986
LmnI GCTCC 2 cut(s) 1366, 1619
Lsp1109I GCAGC 4 cut(s) 1139, 1411, 1985, 1988
LweI GCATC 3 cut(s) 942, 1154, 1394
MaeI CTAG 4 cut(s) 255, 417, 1277, 1787
MaeII ACGT 4 cut(s) 69, 569, 1204, 1372
MaeIII GTNAC 5 cut(s) 25, 250, 368, 429, 1789
MalI GATC 4 cut(s) 6, 975, 1652, 1771
MboI GATC 4 cut(s) 4, 973, 1650, 1769
MfeI CAATTG 1 cut(s) 717
MhlI GDGCHC 3 cut(s) 1036, 1624, 1857
MlyI GAGTC 3 cut(s) 422, 1763, 1786
MseI TTAA 5 cut(s) 59, 477, 911, 1016, 1059
MslI CAYNNNNRTG 2 cut(s) 540, 1039
MspA1I CMGCKG 4 cut(s) 197, 1518, 1560, 1954
MspCI CTTAAG 1 cut(s) 58
MspI CCGG 4 cut(s) 109, 115, 242, 1167
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 8 cut(s) 333, 967, 1073, 1095, 1155, 1217, 1362, 1704
MvnI CGCG 2 cut(s) 197, 1954
NciI CCSGG 2 cut(s) 109, 110
NdeI CATATG 1 cut(s) 1194
NdeII GATC 4 cut(s) 4, 973, 1650, 1769
NlaIII CATG 9 cut(s) 682, 737, 752, 862, 1013, 1180, 1617, 1651, 1908
NlaIV GGNNCC 6 cut(s) 54, 113, 1078, 1368, 1731, 1910
NmuCI GTSAC 3 cut(s) 368, 429, 1789
NspI RCATGY 1 cut(s) 737
NspV TTCGAA 2 cut(s) 461, 467
OliI CACNNNNGTG 1 cut(s) 1039
PaeR7I CTCGAG 1 cut(s) 876
PagI TCATGA 1 cut(s) 1647
PasI CCCWGGG 1 cut(s) 1072
PciI ACATGT 1 cut(s) 733
PciSI GCTCTTC 1 cut(s) 1986
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 538
PfeI GAWTC 7 cut(s) 301, 328, 640, 805, 1597, 1823, 1988
PfoI TCCNGGA 3 cut(s) 1093, 1215, 1702
PinAI ACCGGT 1 cut(s) 1166
PleI GAGTC 3 cut(s) 421, 1762, 1786
PpsI GAGTC 3 cut(s) 421, 1762, 1786
Ppu21I YACGTR 2 cut(s) 1205, 1373
PscI ACATGT 1 cut(s) 733
PshAI GACNNNNGTC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 1624
Psp6I CCWGG 8 cut(s) 331, 965, 1071, 1093, 1153, 1215, 1360, 1702
PspGI CCWGG 8 cut(s) 331, 965, 1071, 1093, 1153, 1215, 1360, 1702
PspN4I GGNNCC 6 cut(s) 54, 113, 1078, 1368, 1731, 1910
PspPI GGNCC 4 cut(s) 112, 1358, 1383, 1909
PspXI VCTCGAGB 1 cut(s) 876
PsrI GAACNNNNNNTAC 2 cut(s) 729, 761
RsaI GTAC 5 cut(s) 897, 1207, 1225, 1484, 1717
RsaNI GTAC 5 cut(s) 896, 1206, 1224, 1483, 1716
RseI CAYNNNNRTG 2 cut(s) 540, 1039
SacI GAGCTC 1 cut(s) 1624
SacII CCGCGG 2 cut(s) 198, 1955
SapI GCTCTTC 1 cut(s) 1986
SaqAI TTAA 5 cut(s) 59, 477, 911, 1016, 1059
Sau3AI GATC 4 cut(s) 4, 973, 1650, 1769
Sau96I GGNCC 4 cut(s) 112, 1358, 1383, 1909
ScaI AGTACT 1 cut(s) 1484
SchI GAGTC 3 cut(s) 422, 1763, 1786
SduI GDGCHC 3 cut(s) 1036, 1624, 1857
SfaNI GCATC 3 cut(s) 942, 1154, 1394
SfcI CTRYAG 1 cut(s) 1410
Sfr274I CTCGAG 1 cut(s) 876
Sfr303I CCGCGG 2 cut(s) 198, 1955
SfuI TTCGAA 2 cut(s) 461, 467
SgrBI CCGCGG 2 cut(s) 198, 1955
SinI GGWCC 2 cut(s) 1358, 1909
SlaI CTCGAG 1 cut(s) 876
SmaI CCCGGG 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 540, 1039
SmlI CTYRAG 2 cut(s) 58, 876
SmoI CTYRAG 2 cut(s) 58, 876
SnaBI TACGTA 1 cut(s) 1205
SpeI ACTAGT 1 cut(s) 1786
SspI AATATT 1 cut(s) 915
SspMI CTAG 4 cut(s) 255, 417, 1277, 1787
SstI GAGCTC 1 cut(s) 1624
StyI CCWWGG 2 cut(s) 1276, 1898
TaaI ACNGT 6 cut(s) 279, 311, 374, 1210, 1390, 1913
TaiI ACGT 4 cut(s) 72, 572, 1207, 1375
TaqI TCGA 8 cut(s) 304, 421, 461, 467, 877, 1113, 1552, 1915
TaqII GACCGA 1 cut(s) 1346
TatI WGTACW 3 cut(s) 895, 1223, 1482
TauI GCSGC 9 cut(s) 132, 171, 194, 197, 200, 1518, 1613, 1954, 1957
TfiI GAWTC 7 cut(s) 301, 328, 640, 805, 1597, 1823, 1988
Tru1I TTAA 5 cut(s) 59, 477, 911, 1016, 1059
Tru9I TTAA 5 cut(s) 59, 477, 911, 1016, 1059
TscAI CASTG 6 cut(s) 774, 1036, 1041, 1129, 1137, 1516
TseFI GTSAC 3 cut(s) 368, 429, 1789
TseI GCWGC 4 cut(s) 1127, 1424, 1973, 1976
Tsp45I GTSAC 3 cut(s) 368, 429, 1789
TspDTI ATGAA 8 cut(s) 567, 737, 794, 822, 1193, 1211, 1815, 1875
TspGWI ACGGA 2 cut(s) 24, 1643
TspMI CCCGGG 1 cut(s) 108
TspRI CASTG 6 cut(s) 774, 1036, 1041, 1129, 1137, 1516
Vha464I CTTAAG 1 cut(s) 58
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 2 cut(s) 1358, 1909
XapI RAATTY 3 cut(s) 118, 463, 1109
XceI RCATGY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 1377
XhoI CTCGAG 1 cut(s) 876
XmaI CCCGGG 1 cut(s) 108
XmaJI CCTAGG 1 cut(s) 1276
XmiI GTMKAC 1 cut(s) 684
XspI CTAG 4 cut(s) 255, 417, 1277, 1787
ZrmI AGTACT 1 cut(s) 1484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.