RchiOBHm_Chr5g0003961

Tyrosyl-DNA phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010258
Physical Location & Seq
Reverse (-)
2466602 .. 2471304
4703 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28524

Sequence Viewer

Length: 1971 bp
ATGGCTGACCTTCTGGTAGGACACTTGGTTCCCTTAAGCAACCACGTCCAAGAAGACGCTTCAATCCCCAAAATACCCCTCTCCCGGGGCCGGAATTCCATCGGCCGCAACTCCGCCCCTTCCCTCACCGACAAGCGTCTCAGCCGCAACCACCTCTCCCTCACCGCCGCCGCGGCCGACGCCTCCTCCGCCGATTTGGTCGTGGAGGGAAGCAATCCGGTTGTGGTAACTAGCGGAAATGATAGGAAGAAACTGTATCATAAAGAAAGTATGACGATTCGAGACGGTGATATTATAGAGCTGATTCCAGGGCAGTATATTTTTCAGTATAGGAAATTGTGTGGTGATAGTTATGAGAAGTGTTCAAATGGTGACGAGAAGGCTGAGGAGTCTAGTCGAAAGCGGTCACATCAGTTAGAATGTGGAGAGAATGGTTCGAATTCGAAGCGTTTTAAGGGTGAGGTGGAAGGAAAGAGTGATGATGGTGGTGGTCGTGTGGAGGCGATACGGCATTTTCGTGTTCCTAATGAAAAGTTGCCACATACGTTTCGGCTTTTGAATGTGCAAGGACTGCCCCCGTGGGCGAATACCTCGTGTGTTTCTATAAAGGATGTGATTCAGGGAGACATCCTTTTTGCTGTCCTTTCCAATTACATGGTAGACCTTGATTGGCTGATACCTGCTTGTCCAACAGTTGCAAAAATCCCACATGTATTGGTTGTTCATGGTGAGGGTGATGGTACACTGGATTATATGAAGAGAAAGAAACCTGCAAATTGGATTCTACACAAACCACCATTGCCTATTTCATTTGGGACACACCACTCAAAGGCCATGCTTCTTGTTTATCCTCGAGGAGTGAGAATAATAGTACATACAGCAAACTTAATATTTGTTGATTGGAACAACAAAAGTCAAGGTTTGTGGATGCAAGATTTTCCCTGGAAAGATCAAAGTAATCCAAGCAAAGGTTGTGGGTTTGAACATGACTTAATTGACTATCTCAGTGCACTGAAGTGGCCTGAGTTTTCTGTTAATTTGCCTACCCTGGGAACCTTCAATATAAATCCAGGATTTTTCAAGAAATTCGATTACAGCAGTGCAGCAGTGAGGCTAATTGCATCTGTCCCTGGATATCATACTGGTAATAACATGAAAAAGTGGGGACATATGAAACTACGTACTGTTCTCCAGGAGTGCACTTTTGATAAAGAGTTTCGGAAATCTCCTCTTGCTTATCAGTTCTCCTCCCTAGGTTCTTTGGATGAGAAATGGATGGCTGAGCTTGCACATTCAATGTCATCAGGTTTTTCAGATGATAAAACTCCTCTCGGTCCTGGGGAGCCACGTATAATATGGCCCACAGTAGAAGATGTCAGATGCTCTATAGAGGGTTATGCTGCTGGAAATGCTGTTCCAAGTCCAGCAAAGAATGTGGATAAAGATTTCTTGAAGAAGTACTGGGCAAGATGGAAGGCAAGCCACACTGGCCGCTGTCGTGCAATGCCACATATAAAGACATTTACTCGATACAGCGGTCAGAAACTTGCTTGGTTTTTGCTAACATCAGCGAATCTTAGCAAAGCTGCATGGGGAGCTCTCCAGAAGAATAATTCTCAACTCATGATCCGTTCTTATGAGCTTGGGGTGCTGTTTCTACCATATAAAACACGAAGTCCTGGATTTTCTTGTACCAGTAATGGGGTTCCATCTGAGGATAAATCTGAAGAGTCAGATAATGCGGGATCAAGGAGAAATAAACTAGTGACTCTGAACTGGCAAGGGAACAGGAACACAGATTCATCATCTGAAGTAATCACATTGCCTGTGCCCTATGAACTCCCTCCTCAACCATACACCACCCAAGATGTTCCTTGGTCATGGGACCGTCGATACACCAAGAAAGATGTTTATGGTCAAGTTTGGCCGCGGCAAGTTCAGCTGTATGCAGCAGCTCTTCAAGATTCTTGA

Protein Analysis

656

Amino Acids

73.56

Weight (kDa)

9.09

Isoelectric Point (pI)

44.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyr-DNA_phospho PF06087 183 - 620 1.2e-102 Tyrosyl-DNA phosphodiesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 852
Acc36I ACCTGC 2 cut(s) 688, 778
AccI GTMKAC 1 cut(s) 660
AccII CGCG 2 cut(s) 173, 1930
AclWI GGATC 2 cut(s) 1621, 1753
AcoI YGGCCR 4 cut(s) 103, 174, 1489, 1925
AcsI RAATTY 3 cut(s) 94, 439, 1085
AcuI CTGAAG 3 cut(s) 1034, 1746, 1830
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 5 cut(s) 742, 873, 1183, 1460, 1693
AfiI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
AflII CTTAAG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 9 cut(s) 63, 366, 559, 983, 1060, 1081, 1296, 1453, 1961
AhlI ACTAGT 1 cut(s) 1762
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
AleI CACNNNNGTG 1 cut(s) 1015
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 1398, 1430
AluBI AGCT 7 cut(s) 301, 1285, 1586, 1598, 1642, 1942, 1955
AluI AGCT 7 cut(s) 301, 1285, 1586, 1598, 1642, 1942, 1955
Alw21I GWGCWC 3 cut(s) 1012, 1202, 1600
Alw26I GTCTC 3 cut(s) 143, 276, 618
Alw44I GTGCAC 2 cut(s) 1008, 1198
AlwI GGATC 2 cut(s) 1621, 1753
Ama87I CYCGRG 2 cut(s) 84, 852
AoxI GGCC 8 cut(s) 88, 103, 174, 831, 1019, 1358, 1489, 1925
ApaLI GTGCAC 2 cut(s) 1008, 1198
ApeKI GCWGC 5 cut(s) 1103, 1400, 1586, 1949, 1952
ApoI RAATTY 3 cut(s) 94, 439, 1085
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AspA2I CCTAGG 1 cut(s) 1252
AspS9I GGNCC 4 cut(s) 88, 1334, 1359, 1885
AsuC2I CCSGG 2 cut(s) 85, 86
AsuHPI GGTGA 8 cut(s) 118, 154, 299, 356, 383, 470, 740, 746
AsuII TTCGAA 2 cut(s) 437, 443
AvaI CYCGRG 2 cut(s) 84, 852
AvaII GGWCC 2 cut(s) 1334, 1885
AvrII CCTAGG 1 cut(s) 1252
BaeGI GKGCMC 3 cut(s) 1012, 1202, 1833
BaeI ACNNNNGTAYC 2 cut(s) 1522, 1555
BanII GRGCYC 1 cut(s) 1600
BauI CACGAG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 3 cut(s) 1012, 1202, 1600
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 5 cut(s) 1115, 1387, 1573, 1961, 1964
BccI CCATC 6 cut(s) 107, 476, 731, 1270, 1464, 1717
BceAI ACGGC 1 cut(s) 524
BciT130I CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
BcnI CCSGG 2 cut(s) 85, 86
BcoDI GTCTC 3 cut(s) 143, 276, 618
BcuI ACTAGT 1 cut(s) 1762
BfaI CTAG 4 cut(s) 231, 393, 1253, 1763
BfmI CTRYAG 1 cut(s) 1386
BfrI CTTAAG 1 cut(s) 34
BfuAI ACCTGC 2 cut(s) 688, 778
BglI GCCNNNNNGGC 2 cut(s) 173, 1488
BlnI CCTAGG 1 cut(s) 1252
BlpI GCTNAGC 1 cut(s) 1281
BmcAI AGTACT 1 cut(s) 1460
Bme18I GGWCC 2 cut(s) 1334, 1885
BmeT110I CYCGRG 2 cut(s) 84, 852
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 4 cut(s) 88, 1334, 1359, 1885
BmiI GGNNCC 6 cut(s) 30, 89, 1054, 1344, 1707, 1886
BmrI ACTGGG 1 cut(s) 1471
BmsI GCATC 3 cut(s) 918, 1130, 1370
BmuI ACTGGG 1 cut(s) 1471
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 2 cut(s) 1175, 1586
Bpu10I CCTNAGC 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 1281
Bpu14I TTCGAA 2 cut(s) 437, 443
BpuMI CCSGG 2 cut(s) 85, 86
BsaAI YACGTR 2 cut(s) 1181, 1349
BsaHI GRCGYC 1 cut(s) 180
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 170, 200
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
Bse1I ACTGG 6 cut(s) 750, 1147, 1466, 1492, 1695, 1781
Bse3DI GCAATG 3 cut(s) 797, 1509, 1820
BseBI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
BseGI GGATG 5 cut(s) 616, 627, 933, 1270, 1281
BseLI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
BseMI GCAATG 3 cut(s) 797, 1509, 1820
BseMII CTCAG 6 cut(s) 154, 375, 1014, 1018, 1272, 1704
BseNI ACTGG 6 cut(s) 750, 1147, 1466, 1492, 1695, 1781
BseRI GAGGAG 7 cut(s) 175, 401, 870, 1218, 1237, 1317, 1836
BseSI GKGCMC 3 cut(s) 1012, 1202, 1833
BseX3I CGGCCG 2 cut(s) 103, 174
BseXI GCAGC 5 cut(s) 1115, 1387, 1573, 1961, 1964
BsgI GTGCAG 1 cut(s) 1122
Bsh1236I CGCG 2 cut(s) 173, 1930
Bsh1285I CGRYCG 2 cut(s) 106, 177
BshFI GGCC 8 cut(s) 90, 105, 176, 833, 1021, 1360, 1491, 1927
BsiEI CGRYCG 2 cut(s) 106, 177
BsiHKAI GWGCWC 3 cut(s) 1012, 1202, 1600
BsiHKCI CYCGRG 2 cut(s) 84, 852
BsiSI CCGG 3 cut(s) 85, 91, 218
BslFI GGGAC 4 cut(s) 829, 1112, 1179, 1898
BslI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
BsmAI GTCTC 3 cut(s) 143, 276, 618
BsmBI CGTCTC 2 cut(s) 143, 276
BsmFI GGGAC 4 cut(s) 829, 1112, 1179, 1898
BsnI GGCC 8 cut(s) 90, 105, 176, 833, 1021, 1360, 1491, 1927
BsoBI CYCGRG 2 cut(s) 84, 852
Bsp119I TTCGAA 2 cut(s) 437, 443
Bsp1286I GDGCHC 4 cut(s) 1012, 1202, 1600, 1833
Bsp143I GATC 3 cut(s) 949, 1626, 1745
Bsp1720I GCTNAGC 1 cut(s) 1281
BspANI GGCC 8 cut(s) 90, 105, 176, 833, 1021, 1360, 1491, 1927
BspCNI CTCAG 6 cut(s) 153, 376, 1015, 1017, 1273, 1705
BspFNI CGCG 2 cut(s) 173, 1930
BspHI TCATGA 1 cut(s) 1623
BspLI GGNNCC 6 cut(s) 30, 89, 1054, 1344, 1707, 1886
BspMI ACCTGC 2 cut(s) 688, 778
BspPI GGATC 2 cut(s) 1621, 1753
BspQI GCTCTTC 1 cut(s) 1962
BspT104I TTCGAA 2 cut(s) 437, 443
BspTI CTTAAG 1 cut(s) 34
BsrDI GCAATG 3 cut(s) 797, 1509, 1820
BsrI ACTGG 6 cut(s) 750, 1147, 1466, 1492, 1695, 1781
BssMI GATC 3 cut(s) 949, 1626, 1745
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 592
BssT1I CCWWGG 2 cut(s) 1252, 1874
Bst2BI CACGAG 1 cut(s) 592
Bst2UI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
Bst4CI ACNGT 6 cut(s) 255, 287, 694, 1186, 1366, 1889
Bst6I CTCTTC 3 cut(s) 752, 1722, 1962
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 571
BstBAI YACGTR 2 cut(s) 1181, 1349
BstBI TTCGAA 2 cut(s) 437, 443
BstC8I GCNNGC 2 cut(s) 1287, 1480
BstDEI CTNAG 7 cut(s) 140, 384, 1004, 1023, 1281, 1577, 1713
BstDSI CCRYGG 3 cut(s) 171, 578, 1928
BstF5I GGATG 5 cut(s) 616, 627, 933, 1270, 1281
BstFNI CGCG 2 cut(s) 173, 1930
BstKTI GATC 3 cut(s) 952, 1629, 1748
BstMAI GTCTC 3 cut(s) 143, 276, 618
BstMBI GATC 3 cut(s) 949, 1626, 1745
BstMCI CGRYCG 2 cut(s) 106, 177
BstNI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
BstNSI RCATGY 1 cut(s) 713
BstPAI GACNNNNGTC 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 1386
BstSLI GKGCMC 3 cut(s) 1012, 1202, 1833
BstSNI TACGTA 1 cut(s) 1181
BstUI CGCG 2 cut(s) 173, 1930
BstV1I GCAGC 5 cut(s) 1115, 1387, 1573, 1961, 1964
BstV2I GAAGAC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 655
BstZI CGGCCG 2 cut(s) 103, 174
BsuRI GGCC 8 cut(s) 90, 105, 176, 833, 1021, 1360, 1491, 1927
BtgI CCRYGG 3 cut(s) 171, 578, 1928
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 5 cut(s) 616, 627, 933, 1270, 1281
BtsI GCAGTG 2 cut(s) 1105, 1113
BtsIMutI CAGTG 6 cut(s) 743, 1010, 1012, 1105, 1113, 1485
BveI ACCTGC 2 cut(s) 688, 778
Cac8I GCNNGC 2 cut(s) 1287, 1480
CciI TCATGA 1 cut(s) 1623
Cfr13I GGNCC 4 cut(s) 88, 1334, 1359, 1885
Cfr42I CCGCGG 2 cut(s) 174, 1931
Cfr9I CCCGGG 1 cut(s) 84
CseI GACGC 3 cut(s) 65, 125, 188
Csp6I GTAC 5 cut(s) 741, 872, 1182, 1459, 1692
CspCI CAANNNNNGTGG 8 cut(s) 696, 731, 905, 940, 955, 990, 1416, 1451
CviAII CATG 9 cut(s) 655, 710, 725, 835, 986, 1153, 1590, 1624, 1881
CviQI GTAC 5 cut(s) 741, 872, 1182, 1459, 1692
DdeI CTNAG 7 cut(s) 140, 384, 1004, 1023, 1281, 1577, 1713
DpnI GATC 3 cut(s) 951, 1628, 1747
DpnII GATC 3 cut(s) 949, 1626, 1745
EaeI YGGCCR 4 cut(s) 103, 174, 1489, 1925
EagI CGGCCG 2 cut(s) 103, 174
Eam1104I CTCTTC 3 cut(s) 752, 1722, 1962
EarI CTCTTC 3 cut(s) 752, 1722, 1962
EciI GGCGGA 2 cut(s) 103, 178
Ecl136II GAGCTC 1 cut(s) 1598
EclXI CGGCCG 2 cut(s) 103, 174
Eco105I TACGTA 1 cut(s) 1181
Eco130I CCWWGG 2 cut(s) 1252, 1874
Eco24I GRGCYC 1 cut(s) 1600
Eco32I GATATC 1 cut(s) 1136
Eco47I GGWCC 2 cut(s) 1334, 1885
Eco52I CGGCCG 2 cut(s) 103, 174
Eco53kI GAGCTC 1 cut(s) 1598
Eco57I CTGAAG 3 cut(s) 1034, 1746, 1830
Eco88I CYCGRG 2 cut(s) 84, 852
EcoICRI GAGCTC 1 cut(s) 1598
EcoRI GAATTC 2 cut(s) 94, 439
EcoRII CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
EcoRV GATATC 1 cut(s) 1136
EcoT14I CCWWGG 2 cut(s) 1252, 1874
EcoT38I GRGCYC 1 cut(s) 1600
ErhI CCWWGG 2 cut(s) 1252, 1874
Esp3I CGTCTC 2 cut(s) 143, 276
FaeI CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1884
FaqI GGGAC 4 cut(s) 829, 1112, 1179, 1898
FatI CATG 9 cut(s) 654, 709, 724, 834, 985, 1152, 1589, 1623, 1880
FauI CCCGC 1 cut(s) 1735
FauNDI CATATG 1 cut(s) 1170
FblI GTMKAC 1 cut(s) 660
FokI GGATG 5 cut(s) 614, 623, 940, 1277, 1288
FriOI GRGCYC 1 cut(s) 1600
FspBI CTAG 4 cut(s) 231, 393, 1253, 1763
GsuI CTGGAG 2 cut(s) 1175, 1586
HaeIII GGCC 8 cut(s) 90, 105, 176, 833, 1021, 1360, 1491, 1927
HapII CCGG 3 cut(s) 85, 91, 218
HgaI GACGC 3 cut(s) 65, 125, 188
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1884
HpaII CCGG 3 cut(s) 85, 91, 218
HphI GGTGA 8 cut(s) 118, 154, 299, 356, 383, 470, 740, 746
Hpy166II GTNNAC 4 cut(s) 661, 743, 1010, 1200
Hpy188I TCNGA 9 cut(s) 1221, 1315, 1379, 1542, 1714, 1726, 1735, 1773, 1810
Hpy188III TCNNGA 7 cut(s) 281, 1081, 1450, 1603, 1624, 1961, 1968
Hpy8I GTNNAC 4 cut(s) 661, 743, 1010, 1200
Hpy99I CGWCG 2 cut(s) 182, 1893
HpyAV CCTTC 6 cut(s) 20, 129, 373, 461, 1066, 1468
HpyCH4III ACNGT 6 cut(s) 255, 287, 694, 1186, 1366, 1889
HpyCH4IV ACGT 4 cut(s) 45, 545, 1180, 1348
HpyF3I CTNAG 7 cut(s) 140, 384, 1004, 1023, 1281, 1577, 1713
HpySE526I ACGT 4 cut(s) 45, 545, 1180, 1348
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1884
KspI CCGCGG 2 cut(s) 174, 1931
Kzo9I GATC 3 cut(s) 949, 1626, 1745
LguI GCTCTTC 1 cut(s) 1962
LmnI GCTCC 2 cut(s) 1342, 1595
Lsp1109I GCAGC 5 cut(s) 1115, 1387, 1573, 1961, 1964
LweI GCATC 3 cut(s) 918, 1130, 1370
MaeI CTAG 4 cut(s) 231, 393, 1253, 1763
MaeII ACGT 4 cut(s) 45, 545, 1180, 1348
MaeIII GTNAC 4 cut(s) 226, 371, 405, 1765
MalI GATC 3 cut(s) 951, 1628, 1747
MboI GATC 3 cut(s) 949, 1626, 1745
MboII GAAGA 8 cut(s) 65, 259, 769, 1382, 1465, 1618, 1739, 1949
MhlI GDGCHC 4 cut(s) 1012, 1202, 1600, 1833
MlyI GAGTC 3 cut(s) 398, 1739, 1762
MmeI TCCRAC 1 cut(s) 713
MseI TTAA 5 cut(s) 35, 453, 887, 992, 1035
MslI CAYNNNNRTG 2 cut(s) 516, 1015
MspA1I CMGCKG 5 cut(s) 173, 1494, 1536, 1930, 1942
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 3 cut(s) 85, 91, 218
MvaI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
MvnI CGCG 2 cut(s) 173, 1930
NciI CCSGG 2 cut(s) 85, 86
NdeI CATATG 1 cut(s) 1170
NdeII GATC 3 cut(s) 949, 1626, 1745
NlaIII CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1884
NlaIV GGNNCC 6 cut(s) 30, 89, 1054, 1344, 1707, 1886
NmuCI GTSAC 3 cut(s) 371, 405, 1765
NspI RCATGY 1 cut(s) 713
NspV TTCGAA 2 cut(s) 437, 443
OliI CACNNNNGTG 1 cut(s) 1015
PaeR7I CTCGAG 1 cut(s) 852
PagI TCATGA 1 cut(s) 1623
PasI CCCWGGG 1 cut(s) 1048
PciI ACATGT 1 cut(s) 709
PciSI GCTCTTC 1 cut(s) 1962
PcsI WCGNNNNNNNCGW 1 cut(s) 514
PfeI GAWTC 7 cut(s) 277, 304, 616, 781, 1573, 1799, 1964
PfoI TCCNGGA 3 cut(s) 1069, 1191, 1678
PleI GAGTC 3 cut(s) 397, 1738, 1762
PpsI GAGTC 3 cut(s) 397, 1738, 1762
Ppu21I YACGTR 2 cut(s) 1181, 1349
PscI ACATGT 1 cut(s) 709
PshAI GACNNNNGTC 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 1600
Psp6I CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
PspGI CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
PspN4I GGNNCC 6 cut(s) 30, 89, 1054, 1344, 1707, 1886
PspPI GGNCC 4 cut(s) 88, 1334, 1359, 1885
PspXI VCTCGAGB 1 cut(s) 852
PsrI GAACNNNNNNTAC 2 cut(s) 705, 737
PvuII CAGCTG 1 cut(s) 1942
RsaI GTAC 5 cut(s) 742, 873, 1183, 1460, 1693
RsaNI GTAC 5 cut(s) 741, 872, 1182, 1459, 1692
RseI CAYNNNNRTG 2 cut(s) 516, 1015
SacI GAGCTC 1 cut(s) 1600
SacII CCGCGG 2 cut(s) 174, 1931
SapI GCTCTTC 1 cut(s) 1962
SaqAI TTAA 5 cut(s) 35, 453, 887, 992, 1035
Sau3AI GATC 3 cut(s) 949, 1626, 1745
Sau96I GGNCC 4 cut(s) 88, 1334, 1359, 1885
ScaI AGTACT 1 cut(s) 1460
SchI GAGTC 3 cut(s) 398, 1739, 1762
SduI GDGCHC 4 cut(s) 1012, 1202, 1600, 1833
SfaNI GCATC 3 cut(s) 918, 1130, 1370
SfcI CTRYAG 1 cut(s) 1386
Sfr274I CTCGAG 1 cut(s) 852
Sfr303I CCGCGG 2 cut(s) 174, 1931
SfuI TTCGAA 2 cut(s) 437, 443
SgrBI CCGCGG 2 cut(s) 174, 1931
SinI GGWCC 2 cut(s) 1334, 1885
SlaI CTCGAG 1 cut(s) 852
SmaI CCCGGG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 516, 1015
SmlI CTYRAG 2 cut(s) 34, 852
SmoI CTYRAG 2 cut(s) 34, 852
SnaBI TACGTA 1 cut(s) 1181
SpeI ACTAGT 1 cut(s) 1762
SspI AATATT 1 cut(s) 891
SspMI CTAG 4 cut(s) 231, 393, 1253, 1763
SstI GAGCTC 1 cut(s) 1600
StyI CCWWGG 2 cut(s) 1252, 1874
TaaI ACNGT 6 cut(s) 255, 287, 694, 1186, 1366, 1889
TaiI ACGT 4 cut(s) 48, 548, 1183, 1351
TaqI TCGA 8 cut(s) 280, 397, 437, 443, 853, 1089, 1528, 1891
TaqII GACCGA 1 cut(s) 1322
TatI WGTACW 2 cut(s) 871, 1458
TauI GCSGC 8 cut(s) 108, 147, 170, 173, 176, 1494, 1930, 1933
TfiI GAWTC 7 cut(s) 277, 304, 616, 781, 1573, 1799, 1964
Tru1I TTAA 5 cut(s) 35, 453, 887, 992, 1035
Tru9I TTAA 5 cut(s) 35, 453, 887, 992, 1035
TscAI CASTG 6 cut(s) 750, 1012, 1017, 1105, 1113, 1492
TseFI GTSAC 3 cut(s) 371, 405, 1765
TseI GCWGC 5 cut(s) 1103, 1400, 1586, 1949, 1952
Tsp45I GTSAC 3 cut(s) 371, 405, 1765
TspDTI ATGAA 8 cut(s) 543, 713, 770, 798, 1169, 1187, 1791, 1851
TspGWI ACGGA 1 cut(s) 1619
TspMI CCCGGG 1 cut(s) 84
TspRI CASTG 6 cut(s) 750, 1012, 1017, 1105, 1113, 1492
Vha464I CTTAAG 1 cut(s) 34
VneI GTGCAC 2 cut(s) 1008, 1198
VpaK11BI GGWCC 2 cut(s) 1334, 1885
XapI RAATTY 3 cut(s) 94, 439, 1085
XceI RCATGY 1 cut(s) 713
XcmI CCANNNNNNNNNTGG 1 cut(s) 1353
XhoI CTCGAG 1 cut(s) 852
XmaI CCCGGG 1 cut(s) 84
XmaJI CCTAGG 1 cut(s) 1252
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 4 cut(s) 231, 393, 1253, 1763
ZrmI AGTACT 1 cut(s) 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.