Rh5AG036200

Tyrosyl-DNA phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0010258
Physical Location & Seq
Reverse (-)
2446034 .. 2450241
4208 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG036200.1

Sequence Viewer

Length: 1755 bp
ATGGCTGACCTTCTGGTAGGACACTTGGTTCCCTTAAGCAACCACGTCCAAGAAGACGCTTCAATCCCCAAAATACCCCTCTCCCGGGGCCGGAATTCCATCGGCCGCAACTCCGCCCCTTCCCTCACCGACAAGCGTCTCAGCCGCAACCACCTCTCCCTCACAGCCGCCGCGGCCGACGCCTTCTCCGCCGATTTGGTCGTGGAGGGAAGCAATCCGGTTGTGGTAACTAGCGGAAATGATAGGAAGAAACTGTATCATAAAGAAAGTATGACGATTCGAGACGGTGATATTATAGAGCTGATTCCAGGGCACTATATTTTTCAGTATAGGAAATTGTGTGGTGACAGTTATGAGAAGTGTTCAAATGGTGAAGAGAAGGCTGAGGAGTCTAGTCGAAAGCGGTCACATCAGTTAGAATGTGGAGAGAATGGTTCGAATTCGAAGCGTTTTAAGGGTGAGGTGGAAGGGAAGAGTGATGATGGTGGTGGTCGTGTGGAGGCGATACAGCATTTTCGTGTTCCTAATGAAAAGTTGCCGCATACTTTTCGGCTTTTGAATGTGCAAGGACTGCCCCCGTGGGCGAATACCTCGTGTGTTTCTATAAAGGATGTGATTCAGGGAGACATCCTTTTTGCTGTCCTTTCCAATTACATGGTAGACCTTGATTGGCTGATACCTGCTTGTCCAACAATTGCAAAAATCCCTCATGTATTGGTTGTTCATGGTGAGGGTGATGGCACACTGGATTATATGAAGAGAAAGAAACCTGCAAATTGGATTCTACACAAACCACCATTGCCTATTTCATTTGGGACACACCACTCAAAGGCCATGCTTCTTGTTTATCCTCGAGGAGTGAGAATAATAGTACATACAGCAAACTTAATATTTGTTGATTGGAACAACAAAAGTCAAGGTTTGTGGATGCAAGATTTTCCCTGGAAAGATCAAAGTAATCCAAGCAAAGGTTGTGGATTTGAACATGACTTAATTGACTATCTCAGTGCACTGAAGTGGCCTGAGTTTTCTATTAATTTGCCTACCCTGGGAACCTTCAATATAAATCCAGGATTTTTCAAGAAATTCGATTACAGCAGTGCAGCAGTGAGGCTAATTGCATCTGTCCCTGGATATCATACCGGTAATAACATGAAAAAGTGGGGACATATGAAACTACGTACTGTTCTCCAGGAGTGTACTTTTGATAAAGAGTTTCGGAAATCTCCTCTTGCTTATCAGTTCTCCTCCCTAGGTTCTTTGGATGAGAAATGGATGGCTGAGCTTGCACATTCAATGTCATCAGGTTTTTCGGATGATAAAACTCCTCTCGGTCCTGGGGAACCACGTATAATATGGCCCACAGTAGAAGATGTTAGATGCTCTATAGAGGGTTATGCTGCTGGAAATGCTGTTCCAAGTCCAGCAAAGAATGTGGATAAAGATTTCTTGAAGAAGTACTGGGCAAGATGGAAGGCAAGCCACACTGGCCGCTGTCGTGCAATGCCACATATAAAGACATTTACTCGATACAGCGGTCAGAAACTTGCTTGGTTTTTGCTAACATCAGCGAATCTTAGCAAAGCTGCATGGGGAGCTCTCCAGAAGAATAATTCTCAACTCATGATCCGTTCTTATGAGCTTGGGGTGCTGTTTCTACCGTATAAAACACGAAGTCCTGGATTTTCTTGTACCAGTAATGGGGTTGCATCTGAGGTTATAGCTACACCCTCACTAGTTTACATGCAATTATAG

Protein Analysis

584

Amino Acids

65.21

Weight (kDa)

9.14

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyr-DNA_phospho PF06087 183 - 555 5e-96 Tyrosyl-DNA phosphodiesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 852
Acc36I ACCTGC 2 cut(s) 688, 778
AccI GTMKAC 1 cut(s) 660
AccII CGCG 1 cut(s) 173
AclWI GGATC 1 cut(s) 1621
AcoI YGGCCR 3 cut(s) 103, 174, 1489
AcsI RAATTY 3 cut(s) 94, 439, 1085
AcuI CTGAAG 1 cut(s) 1034
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 5 cut(s) 873, 1183, 1201, 1460, 1693
AfiI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
AflII CTTAAG 1 cut(s) 34
AgeI ACCGGT 1 cut(s) 1142
AgsI TTSAA 8 cut(s) 63, 366, 559, 983, 1060, 1081, 1296, 1453
AhlI ACTAGT 1 cut(s) 1735
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
AleI CACNNNNGTG 1 cut(s) 1015
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 1398, 1430
AluBI AGCT 6 cut(s) 301, 1285, 1586, 1598, 1642, 1724
AluI AGCT 6 cut(s) 301, 1285, 1586, 1598, 1642, 1724
Alw21I GWGCWC 2 cut(s) 1012, 1600
Alw26I GTCTC 3 cut(s) 143, 276, 618
Alw44I GTGCAC 1 cut(s) 1008
AlwI GGATC 1 cut(s) 1621
Ama87I CYCGRG 2 cut(s) 84, 852
AoxI GGCC 7 cut(s) 88, 103, 174, 831, 1019, 1358, 1489
ApaLI GTGCAC 1 cut(s) 1008
ApeKI GCWGC 3 cut(s) 1103, 1400, 1586
ApoI RAATTY 3 cut(s) 94, 439, 1085
ArsI GACNNNNNNTTYG 2 cut(s) 617, 649
AseI ATTAAT 1 cut(s) 1035
AsiGI ACCGGT 1 cut(s) 1142
AspA2I CCTAGG 1 cut(s) 1252
AspS9I GGNCC 3 cut(s) 88, 1334, 1359
AsuC2I CCSGG 2 cut(s) 85, 86
AsuHPI GGTGA 7 cut(s) 118, 299, 356, 383, 470, 740, 746
AsuII TTCGAA 2 cut(s) 437, 443
AvaI CYCGRG 2 cut(s) 84, 852
AvaII GGWCC 1 cut(s) 1334
AvrII CCTAGG 1 cut(s) 1252
BaeGI GKGCMC 2 cut(s) 315, 1012
BaeI ACNNNNGTAYC 2 cut(s) 1522, 1555
BanII GRGCYC 1 cut(s) 1600
BauI CACGAG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 1012, 1600
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 3 cut(s) 1115, 1387, 1573
BccI CCATC 5 cut(s) 107, 476, 731, 1270, 1464
BcgI CGANNNNNNTGC 2 cut(s) 530, 564
BciT130I CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
BcnI CCSGG 2 cut(s) 85, 86
BcoDI GTCTC 3 cut(s) 143, 276, 618
BcuI ACTAGT 1 cut(s) 1735
BfaI CTAG 4 cut(s) 231, 393, 1253, 1736
BfmI CTRYAG 1 cut(s) 1386
BfrI CTTAAG 1 cut(s) 34
BfuAI ACCTGC 2 cut(s) 688, 778
BglI GCCNNNNNGGC 2 cut(s) 173, 1488
BlnI CCTAGG 1 cut(s) 1252
BlpI GCTNAGC 1 cut(s) 1281
BmcAI AGTACT 1 cut(s) 1460
Bme18I GGWCC 1 cut(s) 1334
BmeT110I CYCGRG 2 cut(s) 84, 852
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 3 cut(s) 88, 1334, 1359
BmiI GGNNCC 4 cut(s) 30, 89, 1054, 1344
BmrI ACTGGG 1 cut(s) 1471
BmsI GCATC 4 cut(s) 918, 1130, 1370, 1718
BmuI ACTGGG 1 cut(s) 1471
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 2 cut(s) 1175, 1586
Bpu10I CCTNAGC 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 1281
Bpu14I TTCGAA 2 cut(s) 437, 443
BpuMI CCSGG 2 cut(s) 85, 86
BsaAI YACGTR 2 cut(s) 1181, 1349
BsaHI GRCGYC 1 cut(s) 180
BsaWI WCCGGW 2 cut(s) 217, 1142
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 170, 200
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
Bse118I RCCGGY 1 cut(s) 1142
Bse1I ACTGG 4 cut(s) 750, 1466, 1492, 1695
Bse3DI GCAATG 2 cut(s) 797, 1509
BseBI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
BseGI GGATG 6 cut(s) 616, 627, 933, 1270, 1281, 1321
BseLI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
BseMI GCAATG 2 cut(s) 797, 1509
BseMII CTCAG 6 cut(s) 154, 375, 1014, 1018, 1272, 1704
BseNI ACTGG 4 cut(s) 750, 1466, 1492, 1695
BseRI GAGGAG 5 cut(s) 401, 870, 1218, 1237, 1317
BseSI GKGCMC 2 cut(s) 315, 1012
BseX3I CGGCCG 2 cut(s) 103, 174
BseXI GCAGC 3 cut(s) 1115, 1387, 1573
BsgI GTGCAG 1 cut(s) 1122
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 2 cut(s) 106, 177
BshFI GGCC 7 cut(s) 90, 105, 176, 833, 1021, 1360, 1491
BshTI ACCGGT 1 cut(s) 1142
BsiEI CGRYCG 2 cut(s) 106, 177
BsiHKAI GWGCWC 2 cut(s) 1012, 1600
BsiHKCI CYCGRG 2 cut(s) 84, 852
BsiSI CCGG 4 cut(s) 85, 91, 218, 1143
BslFI GGGAC 3 cut(s) 829, 1112, 1179
BslI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 829, 968, 1049, 1701
BsmAI GTCTC 3 cut(s) 143, 276, 618
BsmBI CGTCTC 2 cut(s) 143, 276
BsmFI GGGAC 3 cut(s) 829, 1112, 1179
BsnI GGCC 7 cut(s) 90, 105, 176, 833, 1021, 1360, 1491
BsoBI CYCGRG 2 cut(s) 84, 852
Bsp119I TTCGAA 2 cut(s) 437, 443
Bsp1286I GDGCHC 3 cut(s) 315, 1012, 1600
Bsp143I GATC 2 cut(s) 949, 1626
Bsp1720I GCTNAGC 1 cut(s) 1281
BspANI GGCC 7 cut(s) 90, 105, 176, 833, 1021, 1360, 1491
BspCNI CTCAG 6 cut(s) 153, 376, 1015, 1017, 1273, 1705
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 1623
BspLI GGNNCC 4 cut(s) 30, 89, 1054, 1344
BspMI ACCTGC 2 cut(s) 688, 778
BspPI GGATC 1 cut(s) 1621
BspT104I TTCGAA 2 cut(s) 437, 443
BspTI CTTAAG 1 cut(s) 34
BsrDI GCAATG 2 cut(s) 797, 1509
BsrFI RCCGGY 1 cut(s) 1142
BsrI ACTGG 4 cut(s) 750, 1466, 1492, 1695
BssAI RCCGGY 1 cut(s) 1142
BssMI GATC 2 cut(s) 949, 1626
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 592
BssT1I CCWWGG 1 cut(s) 1252
Bst2BI CACGAG 1 cut(s) 592
Bst2UI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
Bst4CI ACNGT 6 cut(s) 255, 287, 350, 1186, 1366, 1662
Bst6I CTCTTC 3 cut(s) 369, 467, 752
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 571
BstBAI YACGTR 2 cut(s) 1181, 1349
BstBI TTCGAA 2 cut(s) 437, 443
BstC8I GCNNGC 2 cut(s) 1287, 1480
BstDEI CTNAG 7 cut(s) 140, 384, 1004, 1023, 1281, 1577, 1713
BstDSI CCRYGG 2 cut(s) 171, 578
BstF5I GGATG 6 cut(s) 616, 627, 933, 1270, 1281, 1321
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 2 cut(s) 952, 1629
BstMAI GTCTC 3 cut(s) 143, 276, 618
BstMBI GATC 2 cut(s) 949, 1626
BstMCI CGRYCG 2 cut(s) 106, 177
BstMWI GCNNNNNNNGC 9 cut(s) 173, 179, 188, 571, 1286, 1409, 1488, 1595, 1648
BstNI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
BstNSI RCATGY 1 cut(s) 1747
BstPAI GACNNNNGTC 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 1386
BstSLI GKGCMC 2 cut(s) 315, 1012
BstSNI TACGTA 1 cut(s) 1181
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 3 cut(s) 1115, 1387, 1573
BstV2I GAAGAC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 655
BstZI CGGCCG 2 cut(s) 103, 174
BsuRI GGCC 7 cut(s) 90, 105, 176, 833, 1021, 1360, 1491
BtgI CCRYGG 2 cut(s) 171, 578
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 6 cut(s) 616, 627, 933, 1270, 1281, 1321
BtsI GCAGTG 2 cut(s) 1105, 1113
BtsIMutI CAGTG 6 cut(s) 743, 1010, 1012, 1105, 1113, 1485
BveI ACCTGC 2 cut(s) 688, 778
Cac8I GCNNGC 2 cut(s) 1287, 1480
CciI TCATGA 1 cut(s) 1623
Cfr10I RCCGGY 1 cut(s) 1142
Cfr13I GGNCC 3 cut(s) 88, 1334, 1359
Cfr42I CCGCGG 1 cut(s) 174
Cfr9I CCCGGG 1 cut(s) 84
CseI GACGC 3 cut(s) 65, 125, 188
Csp6I GTAC 5 cut(s) 872, 1182, 1200, 1459, 1692
CspAI ACCGGT 1 cut(s) 1142
CspCI CAANNNNNGTGG 6 cut(s) 905, 940, 955, 990, 1416, 1451
CviAII CATG 9 cut(s) 655, 710, 725, 835, 986, 1153, 1590, 1624, 1744
CviQI GTAC 5 cut(s) 872, 1182, 1200, 1459, 1692
DdeI CTNAG 7 cut(s) 140, 384, 1004, 1023, 1281, 1577, 1713
DpnI GATC 2 cut(s) 951, 1628
DpnII GATC 2 cut(s) 949, 1626
EaeI YGGCCR 3 cut(s) 103, 174, 1489
EagI CGGCCG 2 cut(s) 103, 174
Eam1104I CTCTTC 3 cut(s) 369, 467, 752
EarI CTCTTC 3 cut(s) 369, 467, 752
EciI GGCGGA 2 cut(s) 103, 178
Ecl136II GAGCTC 1 cut(s) 1598
EclXI CGGCCG 2 cut(s) 103, 174
Eco105I TACGTA 1 cut(s) 1181
Eco130I CCWWGG 1 cut(s) 1252
Eco24I GRGCYC 1 cut(s) 1600
Eco32I GATATC 1 cut(s) 1136
Eco47I GGWCC 1 cut(s) 1334
Eco52I CGGCCG 2 cut(s) 103, 174
Eco53kI GAGCTC 1 cut(s) 1598
Eco57I CTGAAG 1 cut(s) 1034
Eco88I CYCGRG 2 cut(s) 84, 852
EcoICRI GAGCTC 1 cut(s) 1598
EcoRI GAATTC 2 cut(s) 94, 439
EcoRII CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
EcoRV GATATC 1 cut(s) 1136
EcoT14I CCWWGG 1 cut(s) 1252
EcoT38I GRGCYC 1 cut(s) 1600
ErhI CCWWGG 1 cut(s) 1252
Esp3I CGTCTC 2 cut(s) 143, 276
FaeI CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1747
FaqI GGGAC 3 cut(s) 829, 1112, 1179
FatI CATG 9 cut(s) 654, 709, 724, 834, 985, 1152, 1589, 1623, 1743
FauNDI CATATG 1 cut(s) 1170
FblI GTMKAC 1 cut(s) 660
FokI GGATG 6 cut(s) 614, 623, 940, 1277, 1288, 1328
FriOI GRGCYC 1 cut(s) 1600
FspBI CTAG 4 cut(s) 231, 393, 1253, 1736
GsuI CTGGAG 2 cut(s) 1175, 1586
HaeIII GGCC 7 cut(s) 90, 105, 176, 833, 1021, 1360, 1491
HapII CCGG 4 cut(s) 85, 91, 218, 1143
HgaI GACGC 3 cut(s) 65, 125, 188
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1747
HinfI GANTC 6 cut(s) 277, 304, 389, 616, 781, 1573
HpaII CCGG 4 cut(s) 85, 91, 218, 1143
HphI GGTGA 7 cut(s) 118, 299, 356, 383, 470, 740, 746
Hpy166II GTNNAC 4 cut(s) 661, 1010, 1200, 1741
Hpy188I TCNGA 4 cut(s) 1221, 1315, 1542, 1714
Hpy188III TCNNGA 5 cut(s) 281, 1081, 1450, 1603, 1624
Hpy8I GTNNAC 4 cut(s) 661, 1010, 1200, 1741
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 7 cut(s) 20, 129, 193, 373, 461, 1066, 1468
HpyCH4III ACNGT 6 cut(s) 255, 287, 350, 1186, 1366, 1662
HpyCH4IV ACGT 3 cut(s) 45, 1180, 1348
HpyF10VI GCNNNNNNNGC 9 cut(s) 173, 179, 188, 571, 1286, 1409, 1488, 1595, 1648
HpyF3I CTNAG 7 cut(s) 140, 384, 1004, 1023, 1281, 1577, 1713
HpySE526I ACGT 3 cut(s) 45, 1180, 1348
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1747
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 2 cut(s) 949, 1626
LmnI GCTCC 1 cut(s) 1595
Lsp1109I GCAGC 3 cut(s) 1115, 1387, 1573
LweI GCATC 4 cut(s) 918, 1130, 1370, 1718
MaeI CTAG 4 cut(s) 231, 393, 1253, 1736
MaeII ACGT 3 cut(s) 45, 1180, 1348
MaeIII GTNAC 3 cut(s) 226, 344, 405
MalI GATC 2 cut(s) 951, 1628
MboI GATC 2 cut(s) 949, 1626
MboII GAAGA 8 cut(s) 65, 259, 386, 484, 769, 1382, 1465, 1618
MfeI CAATTG 1 cut(s) 693
MhlI GDGCHC 3 cut(s) 315, 1012, 1600
MlyI GAGTC 1 cut(s) 398
MmeI TCCRAC 1 cut(s) 713
MseI TTAA 5 cut(s) 35, 453, 887, 992, 1035
MslI CAYNNNNRTG 2 cut(s) 516, 1015
MspA1I CMGCKG 3 cut(s) 173, 1494, 1536
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 4 cut(s) 85, 91, 218, 1143
MunI CAATTG 1 cut(s) 693
MvaI CCWGG 8 cut(s) 309, 943, 1049, 1071, 1131, 1193, 1338, 1680
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 9 cut(s) 173, 179, 188, 571, 1286, 1409, 1488, 1595, 1648
NciI CCSGG 2 cut(s) 85, 86
NdeI CATATG 1 cut(s) 1170
NdeII GATC 2 cut(s) 949, 1626
NlaIII CATG 9 cut(s) 658, 713, 728, 838, 989, 1156, 1593, 1627, 1747
NlaIV GGNNCC 4 cut(s) 30, 89, 1054, 1344
NmuCI GTSAC 2 cut(s) 344, 405
NspI RCATGY 1 cut(s) 1747
NspV TTCGAA 2 cut(s) 437, 443
OliI CACNNNNGTG 1 cut(s) 1015
PaeR7I CTCGAG 1 cut(s) 852
PagI TCATGA 1 cut(s) 1623
PasI CCCWGGG 1 cut(s) 1048
PfeI GAWTC 5 cut(s) 277, 304, 616, 781, 1573
PfoI TCCNGGA 3 cut(s) 1069, 1191, 1678
PinAI ACCGGT 1 cut(s) 1142
PleI GAGTC 1 cut(s) 397
PpsI GAGTC 1 cut(s) 397
Ppu21I YACGTR 2 cut(s) 1181, 1349
PshAI GACNNNNGTC 1 cut(s) 135
PshBI ATTAAT 1 cut(s) 1035
Psp124BI GAGCTC 1 cut(s) 1600
Psp6I CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
PspGI CCWGG 8 cut(s) 307, 941, 1047, 1069, 1129, 1191, 1336, 1678
PspN4I GGNNCC 4 cut(s) 30, 89, 1054, 1344
PspPI GGNCC 3 cut(s) 88, 1334, 1359
PspXI VCTCGAGB 1 cut(s) 852
PsrI GAACNNNNNNTAC 2 cut(s) 705, 737
RsaI GTAC 5 cut(s) 873, 1183, 1201, 1460, 1693
RsaNI GTAC 5 cut(s) 872, 1182, 1200, 1459, 1692
RseI CAYNNNNRTG 2 cut(s) 516, 1015
SacI GAGCTC 1 cut(s) 1600
SacII CCGCGG 1 cut(s) 174
SaqAI TTAA 5 cut(s) 35, 453, 887, 992, 1035
Sau3AI GATC 2 cut(s) 949, 1626
Sau96I GGNCC 3 cut(s) 88, 1334, 1359
ScaI AGTACT 1 cut(s) 1460
SchI GAGTC 1 cut(s) 398
SduI GDGCHC 3 cut(s) 315, 1012, 1600
SfaNI GCATC 4 cut(s) 918, 1130, 1370, 1718
SfcI CTRYAG 1 cut(s) 1386
Sfr274I CTCGAG 1 cut(s) 852
Sfr303I CCGCGG 1 cut(s) 174
SfuI TTCGAA 2 cut(s) 437, 443
SgrBI CCGCGG 1 cut(s) 174
SinI GGWCC 1 cut(s) 1334
SlaI CTCGAG 1 cut(s) 852
SmaI CCCGGG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 516, 1015
SmlI CTYRAG 2 cut(s) 34, 852
SmoI CTYRAG 2 cut(s) 34, 852
SnaBI TACGTA 1 cut(s) 1181
SpeI ACTAGT 1 cut(s) 1735
SspI AATATT 1 cut(s) 891
SspMI CTAG 4 cut(s) 231, 393, 1253, 1736
SstI GAGCTC 1 cut(s) 1600
StyI CCWWGG 1 cut(s) 1252
TaaI ACNGT 6 cut(s) 255, 287, 350, 1186, 1366, 1662
TaiI ACGT 3 cut(s) 48, 1183, 1351
TaqI TCGA 7 cut(s) 280, 397, 437, 443, 853, 1089, 1528
TaqII GACCGA 1 cut(s) 1322
TatI WGTACW 3 cut(s) 871, 1199, 1458
TauI GCSGC 7 cut(s) 108, 147, 170, 173, 176, 541, 1494
TfiI GAWTC 5 cut(s) 277, 304, 616, 781, 1573
Tru1I TTAA 5 cut(s) 35, 453, 887, 992, 1035
Tru9I TTAA 5 cut(s) 35, 453, 887, 992, 1035
TscAI CASTG 6 cut(s) 750, 1012, 1017, 1105, 1113, 1492
TseFI GTSAC 2 cut(s) 344, 405
TseI GCWGC 3 cut(s) 1103, 1400, 1586
Tsp45I GTSAC 2 cut(s) 344, 405
TspDTI ATGAA 6 cut(s) 543, 713, 770, 798, 1169, 1187
TspGWI ACGGA 1 cut(s) 1619
TspMI CCCGGG 1 cut(s) 84
TspRI CASTG 6 cut(s) 750, 1012, 1017, 1105, 1113, 1492
Vha464I CTTAAG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 1008
VpaK11BI GGWCC 1 cut(s) 1334
VspI ATTAAT 1 cut(s) 1035
XapI RAATTY 3 cut(s) 94, 439, 1085
XceI RCATGY 1 cut(s) 1747
XcmI CCANNNNNNNNNTGG 1 cut(s) 1353
XhoI CTCGAG 1 cut(s) 852
XmaI CCCGGG 1 cut(s) 84
XmaJI CCTAGG 1 cut(s) 1252
XmiI GTMKAC 1 cut(s) 660
XspI CTAG 4 cut(s) 231, 393, 1253, 1736
ZrmI AGTACT 1 cut(s) 1460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.