RLG00000031192

Tyrosyl-DNA phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010258
Physical Location & Seq
Forward (+)
2831940 .. 2835892
3953 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031192

Sequence Viewer

Length: 1242 bp
ATGACATGGGCGTCTCAGTTTAAGTTATCTTCCGAGCTTCATTTAATCATTTGTCTCACTTTTATTACAGTGTATAGGAAATTGTGCGGTGATAGTTATAAGGAGGGTTCAAATGGTGAAGATAAGGCTGAGGAGTCTAGTCGAAAGCGGTTACATCGGTTAGAATGTGGAGAGAATGGTTCGAAATCGAAGCCTTTTAAGACTGATGGTGGGGTGGAGGCGATACGGCATTTTCGTGTTCCTAATGATAAGTTACCGAAGACGTTTCGGCTTTTGAATGTGCAAGGACTGCCCCCGTGGGCGAATACCTCGTGTGTTTCTATAAAGGATGTGATTCAGGGAGACATCCTTTTTGCTGTCCTTTCCAATTACATGGTAGCCCTTGATTGGCTGATACCTGCTTGTCCAACAGTTGCAAAAATCCCACATGTATTGGTTGTTCATGGTGAGGGTGATGGTACACTGGATTATATGAAGAGAAAGATACCTGCAATTTGGATTCTGCACAAACCACCATTGCCTATTTCATTTGGGACACACCATTCAAAGGCCATGCTTCTTGTTTATCCTCGAGGAGTGAGAATACTAGTACATACAGCAAACTTAATATTTGTTGATTGGAACAACAGAAGTCATGGTTTGTGGATTCAAGATTTTCCCTGGAAAGATCAAAGTAATCCAAGCAAAGGTTGTGGATTTGAAAATGACTTAATTGACTATCTCAGTGCACTGAAGTGGCCCGAGTTTTCTGTTAATTTGCCTACCCTGGGAACCTTCAATATAAATCCTGGATTTTTCAAGAAATTTGATTACAGCAATGCAGCAGCTAATTGCATCTGTCCCTGGATATCACACCGGCAACAACATGAAAAGTGGGGACATATGAAACTACGTACTGTTCTCCAGGAGTGTACTTTTGATAAAGACGAAGCATTTATATCTCATTTAATGAAAGCGTTATTTATTTGTATTCTTGCCTTGTTGCAGTTCTCCTCCCTACGTTCTTTGGATGAGAAATGGATGGCTGAGCTTGCACATTCATTGTCATCAGGTTTTTCGGATGATAAAACTCCTCTCGGTCCTGGGGAGCCACGTATAATATGGCCCACAGTAGAAGATGTCAGATGCTCTATAGAGGGTTATGCTGCTGGAAATGCTGTTCCAAGTCCAGTAAAGAATGTGGAGAAAGATTTCTTGAAGAAGTACTGGGCAAGGTGGAAGGCAAGCCACACTGGCCGCTGA

Protein Analysis

414

Amino Acids

46.83

Weight (kDa)

8.83

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyr-DNA_phospho PF06087 89 - 409 1.3e-47 Tyrosyl-DNA phosphodiesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 99
AasI GACNNNNNNGTC 1 cut(s) 10
AbsI CCTCGAGG 1 cut(s) 570
Acc36I ACCTGC 2 cut(s) 406, 496
AciI CCGC 3 cut(s) 87, 148, 1237
AcoI YGGCCR 1 cut(s) 1234
AcsI RAATTY 1 cut(s) 803
AcuI CTGAAG 1 cut(s) 752
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 5 cut(s) 460, 591, 895, 913, 1205
AfiI CCNNNNNNNGG 4 cut(s) 387, 547, 686, 767
AflIII ACRYGT 1 cut(s) 427
AgsI TTSAA 8 cut(s) 111, 277, 546, 650, 701, 778, 799, 1198
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 6 cut(s) 659, 765, 787, 842, 903, 1081
AleI CACNNNNGTG 1 cut(s) 733
AloI GAACNNNNNNTCC 2 cut(s) 1143, 1175
AluBI AGCT 3 cut(s) 37, 827, 1030
AluI AGCT 3 cut(s) 37, 827, 1030
Alw21I GWGCWC 1 cut(s) 730
Alw26I GTCTC 3 cut(s) 18, 59, 336
Alw44I GTGCAC 1 cut(s) 726
Ama87I CYCGRG 2 cut(s) 570, 740
AoxI GGCC 4 cut(s) 549, 737, 1103, 1234
ApaLI GTGCAC 1 cut(s) 726
ApeKI GCWGC 3 cut(s) 821, 824, 1145
ApoI RAATTY 1 cut(s) 803
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
Asp700I GAANNNNTTC 1 cut(s) 1190
AspS9I GGNCC 3 cut(s) 738, 1079, 1104
AsuHPI GGTGA 4 cut(s) 101, 128, 458, 464
AsuII TTCGAA 1 cut(s) 182
AvaI CYCGRG 2 cut(s) 570, 740
AvaII GGWCC 1 cut(s) 1079
BaeGI GKGCMC 1 cut(s) 730
BauI CACGAG 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 266
Bbv12I GWGCWC 1 cut(s) 730
BbvCI CCTCAGC 1 cut(s) 129
BbvI GCAGC 3 cut(s) 833, 836, 1132
BccI CCATC 3 cut(s) 200, 449, 1015
BceAI ACGGC 1 cut(s) 242
BciT130I CCWGG 6 cut(s) 661, 767, 789, 844, 905, 1083
BcoDI GTCTC 3 cut(s) 18, 59, 336
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 2 cut(s) 138, 587
BfmI CTRYAG 1 cut(s) 1131
BfuAI ACCTGC 2 cut(s) 406, 496
BglI GCCNNNNNGGC 1 cut(s) 1233
BisI GCNGC 4 cut(s) 822, 825, 1146, 1237
BlpI GCTNAGC 1 cut(s) 1026
BlsI GCNGC 4 cut(s) 823, 826, 1147, 1238
BmcAI AGTACT 1 cut(s) 1205
Bme1390I CCNGG 6 cut(s) 661, 767, 789, 844, 905, 1083
Bme18I GGWCC 1 cut(s) 1079
BmeT110I CYCGRG 2 cut(s) 570, 740
BmgT120I GGNCC 3 cut(s) 738, 1079, 1104
BmiI GGNNCC 2 cut(s) 772, 1089
BmrFI CCNGG 6 cut(s) 661, 767, 789, 844, 905, 1083
BmrI ACTGGG 1 cut(s) 1216
BmsI GCATC 2 cut(s) 843, 1115
BmuI ACTGGG 1 cut(s) 1216
BpiI GAAGAC 1 cut(s) 266
BpmI CTGGAG 1 cut(s) 887
Bpu10I CCTNAGC 1 cut(s) 129
Bpu1102I GCTNAGC 1 cut(s) 1026
Bpu14I TTCGAA 1 cut(s) 182
BsaAI YACGTR 2 cut(s) 893, 1094
BsaHI GRCGYC 1 cut(s) 11
BsaJI CCNNGG 6 cut(s) 296, 659, 765, 766, 842, 1082
Bsc4I CCNNNNNNNGG 4 cut(s) 387, 547, 686, 767
Bse118I RCCGGY 1 cut(s) 855
Bse1I ACTGG 4 cut(s) 468, 1169, 1211, 1237
Bse3DI GCAATG 2 cut(s) 515, 823
BseBI CCWGG 6 cut(s) 661, 767, 789, 844, 905, 1083
BseDI CCNNGG 6 cut(s) 296, 659, 765, 766, 842, 1082
BseGI GGATG 5 cut(s) 334, 345, 1015, 1026, 1066
BseLI CCNNNNNNNGG 4 cut(s) 387, 547, 686, 767
BseMI GCAATG 2 cut(s) 515, 823
BseMII CTCAG 4 cut(s) 29, 120, 736, 1017
BseNI ACTGG 4 cut(s) 468, 1169, 1211, 1237
BseRI GAGGAG 4 cut(s) 146, 588, 982, 1062
BseSI GKGCMC 1 cut(s) 730
BseXI GCAGC 3 cut(s) 833, 836, 1132
BsgI GTGCAG 1 cut(s) 488
BshFI GGCC 4 cut(s) 551, 739, 1105, 1236
BsiHKAI GWGCWC 1 cut(s) 730
BsiHKCI CYCGRG 2 cut(s) 570, 740
BsiSI CCGG 1 cut(s) 856
BslFI GGGAC 3 cut(s) 547, 825, 891
BslI CCNNNNNNNGG 4 cut(s) 387, 547, 686, 767
BsmAI GTCTC 3 cut(s) 18, 59, 336
BsmBI CGTCTC 1 cut(s) 18
BsmFI GGGAC 3 cut(s) 547, 825, 891
BsnI GGCC 4 cut(s) 551, 739, 1105, 1236
BsoBI CYCGRG 2 cut(s) 570, 740
Bsp119I TTCGAA 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 730
Bsp143I GATC 1 cut(s) 667
Bsp1720I GCTNAGC 1 cut(s) 1026
BspACI CCGC 3 cut(s) 87, 148, 1237
BspANI GGCC 4 cut(s) 551, 739, 1105, 1236
BspCNI CTCAG 4 cut(s) 28, 121, 735, 1018
BspLI GGNNCC 2 cut(s) 772, 1089
BspMI ACCTGC 2 cut(s) 406, 496
BspT104I TTCGAA 1 cut(s) 182
BsrDI GCAATG 2 cut(s) 515, 823
BsrFI RCCGGY 1 cut(s) 855
BsrI ACTGG 4 cut(s) 468, 1169, 1211, 1237
BssAI RCCGGY 1 cut(s) 855
BssECI CCNNGG 6 cut(s) 296, 659, 765, 766, 842, 1082
BssMI GATC 1 cut(s) 667
BssNI GRCGYC 1 cut(s) 11
BssSI CACGAG 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 310
Bst2UI CCWGG 6 cut(s) 661, 767, 789, 844, 905, 1083
Bst4CI ACNGT 4 cut(s) 70, 412, 898, 1111
Bst6I CTCTTC 1 cut(s) 470
BstACI GRCGYC 1 cut(s) 11
BstAPI GCANNNNNTGC 1 cut(s) 289
BstBAI YACGTR 2 cut(s) 893, 1094
BstBI TTCGAA 1 cut(s) 182
BstC8I GCNNGC 2 cut(s) 1032, 1225
BstDEI CTNAG 4 cut(s) 15, 129, 722, 1026
BstDSI CCRYGG 1 cut(s) 296
BstF5I GGATG 5 cut(s) 334, 345, 1015, 1026, 1066
BstKTI GATC 1 cut(s) 670
BstMAI GTCTC 3 cut(s) 18, 59, 336
BstMBI GATC 1 cut(s) 667
BstMWI GCNNNNNNNGC 4 cut(s) 289, 1031, 1154, 1233
BstNI CCWGG 6 cut(s) 661, 767, 789, 844, 905, 1083
BstNSI RCATGY 1 cut(s) 431
BstSCI CCNGG 6 cut(s) 659, 765, 787, 842, 903, 1081
BstSFI CTRYAG 1 cut(s) 1131
BstSLI GKGCMC 1 cut(s) 730
BstSNI TACGTA 1 cut(s) 893
BstV1I GCAGC 3 cut(s) 833, 836, 1132
BstV2I GAAGAC 1 cut(s) 266
BstXI CCANNNNNNTGG 1 cut(s) 373
BsuRI GGCC 4 cut(s) 551, 739, 1105, 1236
BtgI CCRYGG 1 cut(s) 296
BtsCI GGATG 5 cut(s) 334, 345, 1015, 1026, 1066
BtsIMutI CAGTG 5 cut(s) 75, 461, 728, 730, 1230
BveI ACCTGC 2 cut(s) 406, 496
Cac8I GCNNGC 2 cut(s) 1032, 1225
Cfr10I RCCGGY 1 cut(s) 855
Cfr13I GGNCC 3 cut(s) 738, 1079, 1104
Csp6I GTAC 5 cut(s) 459, 590, 894, 912, 1204
CspCI CAANNNNNGTGG 4 cut(s) 414, 449, 673, 708
CviAII CATG 7 cut(s) 6, 373, 428, 443, 553, 635, 866
CviQI GTAC 5 cut(s) 459, 590, 894, 912, 1204
DdeI CTNAG 4 cut(s) 15, 129, 722, 1026
DpnI GATC 1 cut(s) 669
DpnII GATC 1 cut(s) 667
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
EaeI YGGCCR 1 cut(s) 1234
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
Eco105I TACGTA 1 cut(s) 893
Eco32I GATATC 1 cut(s) 849
Eco47I GGWCC 1 cut(s) 1079
Eco57I CTGAAG 1 cut(s) 752
Eco88I CYCGRG 2 cut(s) 570, 740
EcoRII CCWGG 6 cut(s) 659, 765, 787, 842, 903, 1081
EcoRV GATATC 1 cut(s) 849
Esp3I CGTCTC 1 cut(s) 18
FaeI CATG 7 cut(s) 9, 376, 431, 446, 556, 638, 869
FaqI GGGAC 3 cut(s) 547, 825, 891
FatI CATG 7 cut(s) 5, 372, 427, 442, 552, 634, 865
FauNDI CATATG 1 cut(s) 882
Fnu4HI GCNGC 4 cut(s) 822, 825, 1146, 1237
FokI GGATG 5 cut(s) 332, 341, 1022, 1033, 1073
Fsp4HI GCNGC 4 cut(s) 822, 825, 1146, 1237
FspBI CTAG 2 cut(s) 138, 587
GluI GCNGC 4 cut(s) 822, 825, 1146, 1237
GsuI CTGGAG 1 cut(s) 887
HaeIII GGCC 4 cut(s) 551, 739, 1105, 1236
HapII CCGG 1 cut(s) 856
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 7 cut(s) 9, 376, 431, 446, 556, 638, 869
HinfI GANTC 4 cut(s) 134, 334, 499, 646
HpaII CCGG 1 cut(s) 856
HphI GGTGA 4 cut(s) 101, 128, 458, 464
Hpy166II GTNNAC 3 cut(s) 461, 728, 912
Hpy188I TCNGA 3 cut(s) 34, 1060, 1124
Hpy188III TCNNGA 3 cut(s) 650, 799, 1195
Hpy8I GTNNAC 3 cut(s) 461, 728, 912
HpyAV CCTTC 2 cut(s) 784, 1213
HpyCH4III ACNGT 4 cut(s) 70, 412, 898, 1111
HpyCH4IV ACGT 4 cut(s) 263, 892, 1000, 1093
HpyCH4V TGCA 9 cut(s) 283, 416, 491, 505, 728, 821, 834, 985, 1034
HpyF10VI GCNNNNNNNGC 4 cut(s) 289, 1031, 1154, 1233
HpyF3I CTNAG 4 cut(s) 15, 129, 722, 1026
HpySE526I ACGT 4 cut(s) 263, 892, 1000, 1093
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 7 cut(s) 9, 376, 431, 446, 556, 638, 869
Kzo9I GATC 1 cut(s) 667
LmnI GCTCC 1 cut(s) 1087
Lsp1109I GCAGC 3 cut(s) 833, 836, 1132
LweI GCATC 2 cut(s) 843, 1115
MaeI CTAG 2 cut(s) 138, 587
MaeII ACGT 4 cut(s) 263, 892, 1000, 1093
MaeIII GTNAC 2 cut(s) 150, 252
MalI GATC 1 cut(s) 669
MboI GATC 1 cut(s) 667
MboII GAAGA 6 cut(s) 21, 131, 271, 487, 1127, 1210
MhlI GDGCHC 1 cut(s) 730
MluCI AATT 7 cut(s) 80, 367, 492, 711, 754, 803, 829
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 431
MroXI GAANNNNTTC 1 cut(s) 1190
MseI TTAA 7 cut(s) 21, 44, 198, 605, 710, 753, 947
MslI CAYNNNNRTG 2 cut(s) 234, 733
MspA1I CMGCKG 1 cut(s) 1239
MspI CCGG 1 cut(s) 856
MspR9I CCNGG 6 cut(s) 661, 767, 789, 844, 905, 1083
MvaI CCWGG 6 cut(s) 661, 767, 789, 844, 905, 1083
MwoI GCNNNNNNNGC 4 cut(s) 289, 1031, 1154, 1233
NdeI CATATG 1 cut(s) 882
NdeII GATC 1 cut(s) 667
NlaIII CATG 7 cut(s) 9, 376, 431, 446, 556, 638, 869
NlaIV GGNNCC 2 cut(s) 772, 1089
NspI RCATGY 1 cut(s) 431
NspV TTCGAA 1 cut(s) 182
OliI CACNNNNGTG 1 cut(s) 733
PaeR7I CTCGAG 1 cut(s) 570
PasI CCCWGGG 1 cut(s) 766
PciI ACATGT 1 cut(s) 427
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 1190
PfeI GAWTC 3 cut(s) 334, 499, 646
PfoI TCCNGGA 2 cut(s) 787, 903
PkrI GCNGC 4 cut(s) 823, 826, 1147, 1238
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
Ppu21I YACGTR 2 cut(s) 893, 1094
PscI ACATGT 1 cut(s) 427
PsiI TTATAA 1 cut(s) 99
Psp6I CCWGG 6 cut(s) 659, 765, 787, 842, 903, 1081
PspGI CCWGG 6 cut(s) 659, 765, 787, 842, 903, 1081
PspN4I GGNNCC 2 cut(s) 772, 1089
PspPI GGNCC 3 cut(s) 738, 1079, 1104
PspXI VCTCGAGB 1 cut(s) 570
PsrI GAACNNNNNNTAC 2 cut(s) 423, 455
RsaI GTAC 5 cut(s) 460, 591, 895, 913, 1205
RsaNI GTAC 5 cut(s) 459, 590, 894, 912, 1204
RseI CAYNNNNRTG 2 cut(s) 234, 733
SaqAI TTAA 7 cut(s) 21, 44, 198, 605, 710, 753, 947
SatI GCNGC 4 cut(s) 822, 825, 1146, 1237
Sau3AI GATC 1 cut(s) 667
Sau96I GGNCC 3 cut(s) 738, 1079, 1104
ScaI AGTACT 1 cut(s) 1205
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 6 cut(s) 661, 767, 789, 844, 905, 1083
SduI GDGCHC 1 cut(s) 730
SfaNI GCATC 2 cut(s) 843, 1115
SfcI CTRYAG 1 cut(s) 1131
Sfr274I CTCGAG 1 cut(s) 570
SfuI TTCGAA 1 cut(s) 182
SinI GGWCC 1 cut(s) 1079
SlaI CTCGAG 1 cut(s) 570
SmiMI CAYNNNNRTG 2 cut(s) 234, 733
SmlI CTYRAG 1 cut(s) 570
SmoI CTYRAG 1 cut(s) 570
SnaBI TACGTA 1 cut(s) 893
SpeI ACTAGT 1 cut(s) 586
Sse9I AATT 7 cut(s) 80, 367, 492, 711, 754, 803, 829
SsiI CCGC 3 cut(s) 87, 148, 1237
SspI AATATT 1 cut(s) 609
SspMI CTAG 2 cut(s) 138, 587
StyD4I CCNGG 6 cut(s) 659, 765, 787, 842, 903, 1081
TaaI ACNGT 4 cut(s) 70, 412, 898, 1111
TaiI ACGT 4 cut(s) 266, 895, 1003, 1096
TaqI TCGA 4 cut(s) 142, 182, 188, 571
TaqII GACCGA 1 cut(s) 1067
TasI AATT 7 cut(s) 80, 367, 492, 711, 754, 803, 829
TatI WGTACW 3 cut(s) 589, 911, 1203
TauI GCSGC 1 cut(s) 1239
TfiI GAWTC 3 cut(s) 334, 499, 646
Tru1I TTAA 7 cut(s) 21, 44, 198, 605, 710, 753, 947
Tru9I TTAA 7 cut(s) 21, 44, 198, 605, 710, 753, 947
TscAI CASTG 5 cut(s) 75, 468, 730, 735, 1237
TseI GCWGC 3 cut(s) 821, 824, 1145
TspDTI ATGAA 8 cut(s) 29, 431, 488, 516, 882, 899, 965, 1029
TspRI CASTG 5 cut(s) 75, 468, 730, 735, 1237
VneI GTGCAC 1 cut(s) 726
VpaK11BI GGWCC 1 cut(s) 1079
XapI RAATTY 1 cut(s) 803
XceI RCATGY 1 cut(s) 431
XcmI CCANNNNNNNNNTGG 1 cut(s) 1098
XhoI CTCGAG 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 1190
XspI CTAG 2 cut(s) 138, 587
ZrmI AGTACT 1 cut(s) 1205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.