RLG00000031190

Tyrosyl-DNA phosphodiesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010258
Physical Location & Seq
Reverse (-)
2805585 .. 2810271
4687 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031190

Sequence Viewer

Length: 1974 bp
ATGGCTGACCTTCTGGTAGGACACTTGGTTCCCTTAAGCAACCACGTCCAAGAAGACGCTTCAATCCCCAAAATACCCCTCTCCCGGGGCCGGAATTCCATCGGCCGCAACTCCGCCCCTTCCCTCACCGACAAGCGTCTCAGCCGCAACCACCTCTCCCTCACCGCCGCCGCGGCCGACGCCTCCTCCGCCGATTTGGTCGTGGAGGGAAGCAATCCGGTTGTGGTAACTAGCGGAAATGATAGGAAGAAACTGTATCATAAAGAAAGTGTGACGATTCGAGACGGTGATATTATAGAGCTGATTCCAGGGCGGTATATTTTTCAGTATAGGAAATTGTGTGGTGATAGTTATGAGAAGTGTTCAAATGGTGAAGAGAAGGATGAGGAGTCTAGTCGAAAGCGGTTGCATCAGTTAGAATGTGGGGAGAATGGTTCGAATTCGAAGCGTTTTAAGGGTGAGGTGGAAGGGAAGAGTGATGATGGTGGTGGTGGTCGTGTGGAGGCGATACGGCATTTTCGTGTTCCTAAGGATAAGTTACCACAGACGTTTCGGCTTTTGAATGTGCAAGGACTGCCCCCGTGGGCGAATACCTCGTGTGTTTCTATAAAGGATGTCATTCAGGGAGACATCCTTTTTGCTGTCCTTTCCAATTACATGGTAGACCTTGATTGGCTGATACCTGCTTGTCCAACAGTTGCAAAAATCCCACATGTATTGGTTGTTCATGGTGAGGGTGATGGTACACTGGATTATATGAAGAGAAAGAAACCTGCAAATTGGATTCTACACAAACCACCATTGCCTATTTCATTTGGGACACACCACTCAAAGGCCATGCTTCTTGTTTATCCTCGAGGAGTGAGAATAATAGTACATACAGCAAACTTAATATTTGTTGATTGGAACAACAAAAGTCAAGGTTTGTGGATGCAAGATTTTCCCTGGAAAGATCAAAGTAATCCAAGCAAAGGTTGTGGATTTGAAAATGACTTAATTGACTATCTCAGTGCACTGAAGTGGCCCGAGTTTTCTGTTAATTTGCCTACCCTGGGAACCTTCAATATAAATCCAGGATTTTTCAAGAAATTCGATTACAGCAGTGCAGCAGTGAGGCTAATTGCATCTGTCCCTGGATATCATACCGGTAATAACATGAAAAAGTGGGGACATATGAAACTACGTACTGTTCTCCAGGAGTGTACTTTCGATAAAGAGTTTCGGAAATCTCCTCTTGCTTATCAGTTCTCCTCCCTAGGTTCTTTGGATGAGAAATGGATGGCTGAGCTTGCACATTCAATGTCATCAGGTTTTTCGGATGATAAAACTCCTCTCGGTCCTGGGGAGCCACGTATGATATGGCCCACAGTAGAAGATGTCAGATGCTCTATAGAGGGTTATGCTGCTGGAAATGCTGTTCCAAGTCCAGCAAAGAATGTGGAGAAAGATTTCTTGAAGAAGTACTGGGCAAGATGGAAGGCAAGCCATACTGGCCGCTGTCGTGCAATGCCACATATAAAGACATTTACTCGATACAGCGGTCAGAAACTTGCTTGGTTTTTGCTAACATCAGCGAATCTTAGCAAAGCTGCATGGGGAGCTCTCCAGAAGAATAATTCTCAACTCATGATCCGTTCTTATGAGCTTGGGGTGCTGTTTCTACCATATAAAACACGAAGTTCTGGATTTTCTTGTACCAGTAATGGGGTTCCATCTGAGGATAAATCTGAAGAGTCAGATAATTCGGAATCAAGGAGAAATAAACTAGTGACTCTGAACTGGCAAGGGAACAGGAACGCAGATTCATCATCTGAAGTAATCACATTGCCTGTGCCCTATGAACTCCCTCCTCAACCATACACCACCCAAGATGTTCCTTGGTCATGGGACCGGCGATACACCAAGAAAGATGTTTATGGTCAAGTTTGGCCTCGGCAAGTTCAGCTGTATGCAGCAGCTCTTCAAGATTCTTGA

Protein Analysis

658

Amino Acids

73.74

Weight (kDa)

9.09

Isoelectric Point (pI)

44.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tyr-DNA_phospho PF06087 184 - 621 1.7e-102 Tyrosyl-DNA phosphodiesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 855
Acc36I ACCTGC 2 cut(s) 691, 781
AccI GTMKAC 1 cut(s) 663
AccII CGCG 1 cut(s) 173
AclWI GGATC 1 cut(s) 1624
AcoI YGGCCR 3 cut(s) 103, 174, 1492
AcsI RAATTY 3 cut(s) 94, 439, 1088
AcuI CTGAAG 3 cut(s) 1037, 1749, 1833
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 6 cut(s) 745, 876, 1186, 1204, 1463, 1696
AfiI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 832, 971, 1052, 1704
AflII CTTAAG 1 cut(s) 34
AflIII ACRYGT 1 cut(s) 712
AgeI ACCGGT 1 cut(s) 1145
AgsI TTSAA 9 cut(s) 63, 366, 562, 986, 1063, 1084, 1299, 1456, 1964
AhlI ACTAGT 1 cut(s) 1765
AjiI CACGTC 1 cut(s) 46
AjnI CCWGG 7 cut(s) 307, 944, 1050, 1072, 1132, 1194, 1339
AleI CACNNNNGTG 1 cut(s) 1018
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 1401, 1433
AluBI AGCT 7 cut(s) 301, 1288, 1589, 1601, 1645, 1945, 1958
AluI AGCT 7 cut(s) 301, 1288, 1589, 1601, 1645, 1945, 1958
Alw21I GWGCWC 2 cut(s) 1015, 1603
Alw26I GTCTC 3 cut(s) 143, 276, 621
Alw44I GTGCAC 1 cut(s) 1011
AlwI GGATC 1 cut(s) 1624
Ama87I CYCGRG 3 cut(s) 84, 855, 1025
AoxI GGCC 8 cut(s) 88, 103, 174, 834, 1022, 1361, 1492, 1928
ApaLI GTGCAC 1 cut(s) 1011
ApeKI GCWGC 5 cut(s) 1106, 1403, 1589, 1952, 1955
ApoI RAATTY 3 cut(s) 94, 439, 1088
ArsI GACNNNNNNTTYG 2 cut(s) 620, 652
AsiGI ACCGGT 1 cut(s) 1145
Asp700I GAANNNNTTC 1 cut(s) 1448
AspA2I CCTAGG 1 cut(s) 1255
AspS9I GGNCC 5 cut(s) 88, 1023, 1337, 1362, 1888
AsuC2I CCSGG 2 cut(s) 85, 86
AsuHPI GGTGA 8 cut(s) 118, 154, 299, 356, 383, 470, 743, 749
AsuII TTCGAA 2 cut(s) 437, 443
AvaI CYCGRG 3 cut(s) 84, 855, 1025
AvaII GGWCC 2 cut(s) 1337, 1888
AvrII CCTAGG 1 cut(s) 1255
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 2 cut(s) 1015, 1836
BaeI ACNNNNGTAYC 2 cut(s) 1525, 1558
BanII GRGCYC 1 cut(s) 1603
BauI CACGAG 1 cut(s) 595
BbsI GAAGAC 1 cut(s) 60
Bbv12I GWGCWC 2 cut(s) 1015, 1603
BbvI GCAGC 5 cut(s) 1118, 1390, 1576, 1964, 1967
BccI CCATC 6 cut(s) 107, 476, 734, 1273, 1467, 1720
BceAI ACGGC 1 cut(s) 527
BciT130I CCWGG 7 cut(s) 309, 946, 1052, 1074, 1134, 1196, 1341
BcnI CCSGG 2 cut(s) 85, 86
BcoDI GTCTC 3 cut(s) 143, 276, 621
BcuI ACTAGT 1 cut(s) 1765
BfaI CTAG 4 cut(s) 231, 393, 1256, 1766
BfmI CTRYAG 1 cut(s) 1389
BfrI CTTAAG 1 cut(s) 34
BfuAI ACCTGC 2 cut(s) 691, 781
BglI GCCNNNNNGGC 2 cut(s) 173, 1491
BlnI CCTAGG 1 cut(s) 1255
BlpI GCTNAGC 1 cut(s) 1284
BmcAI AGTACT 1 cut(s) 1463
Bme1390I CCNGG 9 cut(s) 85, 86, 309, 946, 1052, 1074, 1134, 1196, 1341
Bme18I GGWCC 2 cut(s) 1337, 1888
BmeT110I CYCGRG 3 cut(s) 84, 855, 1025
BmgBI CACGTC 1 cut(s) 46
BmgT120I GGNCC 5 cut(s) 88, 1023, 1337, 1362, 1888
BmiI GGNNCC 6 cut(s) 30, 89, 1057, 1347, 1710, 1889
BmrFI CCNGG 9 cut(s) 85, 86, 309, 946, 1052, 1074, 1134, 1196, 1341
BmrI ACTGGG 1 cut(s) 1474
BmsI GCATC 4 cut(s) 418, 921, 1133, 1373
BmuI ACTGGG 1 cut(s) 1474
BoxI GACNNNNGTC 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 60
BpmI CTGGAG 2 cut(s) 1178, 1589
Bpu1102I GCTNAGC 1 cut(s) 1284
Bpu14I TTCGAA 2 cut(s) 437, 443
BpuMI CCSGG 2 cut(s) 85, 86
BsaAI YACGTR 2 cut(s) 1184, 1352
BsaHI GRCGYC 1 cut(s) 180
BsaWI WCCGGW 2 cut(s) 217, 1145
BsaXI ACNNNNNCTCC 4 cut(s) 140, 170, 170, 200
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 85, 90, 832, 971, 1052, 1704
Bse118I RCCGGY 2 cut(s) 1145, 1890
Bse1I ACTGG 5 cut(s) 753, 1469, 1495, 1698, 1784
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 800, 1512, 1823
BseBI CCWGG 7 cut(s) 309, 946, 1052, 1074, 1134, 1196, 1341
BseGI GGATG 7 cut(s) 388, 619, 630, 936, 1273, 1284, 1324
BseLI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 832, 971, 1052, 1704
BseMI GCAATG 3 cut(s) 800, 1512, 1823
BseMII CTCAG 4 cut(s) 154, 1021, 1275, 1707
BseNI ACTGG 5 cut(s) 753, 1469, 1495, 1698, 1784
BseRI GAGGAG 7 cut(s) 175, 401, 873, 1221, 1240, 1320, 1839
BseSI GKGCMC 2 cut(s) 1015, 1836
BseX3I CGGCCG 2 cut(s) 103, 174
BseXI GCAGC 5 cut(s) 1118, 1390, 1576, 1964, 1967
BsgI GTGCAG 1 cut(s) 1125
Bsh1236I CGCG 1 cut(s) 173
Bsh1285I CGRYCG 2 cut(s) 106, 177
BshFI GGCC 8 cut(s) 90, 105, 176, 836, 1024, 1363, 1494, 1930
BshTI ACCGGT 1 cut(s) 1145
BsiEI CGRYCG 2 cut(s) 106, 177
BsiHKAI GWGCWC 2 cut(s) 1015, 1603
BsiHKCI CYCGRG 3 cut(s) 84, 855, 1025
BsiSI CCGG 5 cut(s) 85, 91, 218, 1146, 1891
BslFI GGGAC 4 cut(s) 832, 1115, 1182, 1901
BslI CCNNNNNNNGG 7 cut(s) 84, 85, 90, 832, 971, 1052, 1704
BsmAI GTCTC 3 cut(s) 143, 276, 621
BsmBI CGTCTC 2 cut(s) 143, 276
BsmFI GGGAC 4 cut(s) 832, 1115, 1182, 1901
BsnI GGCC 8 cut(s) 90, 105, 176, 836, 1024, 1363, 1494, 1930
BsoBI CYCGRG 3 cut(s) 84, 855, 1025
Bsp119I TTCGAA 2 cut(s) 437, 443
Bsp1286I GDGCHC 3 cut(s) 1015, 1603, 1836
Bsp143I GATC 2 cut(s) 952, 1629
Bsp1720I GCTNAGC 1 cut(s) 1284
BspANI GGCC 8 cut(s) 90, 105, 176, 836, 1024, 1363, 1494, 1930
BspCNI CTCAG 4 cut(s) 153, 1020, 1276, 1708
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 1 cut(s) 1626
BspLI GGNNCC 6 cut(s) 30, 89, 1057, 1347, 1710, 1889
BspMI ACCTGC 2 cut(s) 691, 781
BspPI GGATC 1 cut(s) 1624
BspQI GCTCTTC 1 cut(s) 1965
BspT104I TTCGAA 2 cut(s) 437, 443
BspTI CTTAAG 1 cut(s) 34
BsrDI GCAATG 3 cut(s) 800, 1512, 1823
BsrFI RCCGGY 2 cut(s) 1145, 1890
BsrI ACTGG 5 cut(s) 753, 1469, 1495, 1698, 1784
BssAI RCCGGY 2 cut(s) 1145, 1890
BssMI GATC 2 cut(s) 952, 1629
BssNI GRCGYC 1 cut(s) 180
BssSI CACGAG 1 cut(s) 595
BssT1I CCWWGG 2 cut(s) 1255, 1877
Bst2BI CACGAG 1 cut(s) 595
Bst2UI CCWGG 7 cut(s) 309, 946, 1052, 1074, 1134, 1196, 1341
Bst4CI ACNGT 5 cut(s) 255, 287, 697, 1189, 1369
Bst6I CTCTTC 5 cut(s) 369, 467, 755, 1725, 1965
BstACI GRCGYC 1 cut(s) 180
BstAFI CTTAAG 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 574
BstBAI YACGTR 2 cut(s) 1184, 1352
BstBI TTCGAA 2 cut(s) 437, 443
BstC8I GCNNGC 2 cut(s) 1290, 1483
BstDEI CTNAG 6 cut(s) 140, 528, 1007, 1284, 1580, 1716
BstDSI CCRYGG 2 cut(s) 171, 581
BstF5I GGATG 7 cut(s) 388, 619, 630, 936, 1273, 1284, 1324
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 2 cut(s) 955, 1632
BstMAI GTCTC 3 cut(s) 143, 276, 621
BstMBI GATC 2 cut(s) 952, 1629
BstMCI CGRYCG 2 cut(s) 106, 177
BstNI CCWGG 7 cut(s) 309, 946, 1052, 1074, 1134, 1196, 1341
BstNSI RCATGY 1 cut(s) 716
BstPAI GACNNNNGTC 1 cut(s) 135
BstSCI CCNGG 9 cut(s) 83, 84, 307, 944, 1050, 1072, 1132, 1194, 1339
BstSFI CTRYAG 1 cut(s) 1389
BstSLI GKGCMC 2 cut(s) 1015, 1836
BstSNI TACGTA 1 cut(s) 1184
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 5 cut(s) 1118, 1390, 1576, 1964, 1967
BstV2I GAAGAC 1 cut(s) 60
BstXI CCANNNNNNTGG 1 cut(s) 658
BstZI CGGCCG 2 cut(s) 103, 174
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 8 cut(s) 90, 105, 176, 836, 1024, 1363, 1494, 1930
BtgI CCRYGG 2 cut(s) 171, 581
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 7 cut(s) 388, 619, 630, 936, 1273, 1284, 1324
BtsI GCAGTG 2 cut(s) 1108, 1116
BtsIMutI CAGTG 5 cut(s) 746, 1013, 1015, 1108, 1116
BveI ACCTGC 2 cut(s) 691, 781
Cac8I GCNNGC 2 cut(s) 1290, 1483
CciI TCATGA 1 cut(s) 1626
Cfr10I RCCGGY 2 cut(s) 1145, 1890
Cfr13I GGNCC 5 cut(s) 88, 1023, 1337, 1362, 1888
Cfr42I CCGCGG 1 cut(s) 174
Cfr9I CCCGGG 1 cut(s) 84
CseI GACGC 3 cut(s) 65, 125, 188
Csp6I GTAC 6 cut(s) 744, 875, 1185, 1203, 1462, 1695
CspAI ACCGGT 1 cut(s) 1145
CspCI CAANNNNNGTGG 8 cut(s) 699, 734, 908, 943, 958, 993, 1419, 1454
CviAII CATG 8 cut(s) 658, 713, 728, 838, 1156, 1593, 1627, 1884
CviQI GTAC 6 cut(s) 744, 875, 1185, 1203, 1462, 1695
DdeI CTNAG 6 cut(s) 140, 528, 1007, 1284, 1580, 1716
DpnI GATC 2 cut(s) 954, 1631
DpnII GATC 2 cut(s) 952, 1629
EaeI YGGCCR 3 cut(s) 103, 174, 1492
EagI CGGCCG 2 cut(s) 103, 174
Eam1104I CTCTTC 5 cut(s) 369, 467, 755, 1725, 1965
EarI CTCTTC 5 cut(s) 369, 467, 755, 1725, 1965
EciI GGCGGA 2 cut(s) 103, 178
Ecl136II GAGCTC 1 cut(s) 1601
EclXI CGGCCG 2 cut(s) 103, 174
Eco105I TACGTA 1 cut(s) 1184
Eco130I CCWWGG 2 cut(s) 1255, 1877
Eco24I GRGCYC 1 cut(s) 1603
Eco32I GATATC 1 cut(s) 1139
Eco47I GGWCC 2 cut(s) 1337, 1888
Eco52I CGGCCG 2 cut(s) 103, 174
Eco53kI GAGCTC 1 cut(s) 1601
Eco57I CTGAAG 3 cut(s) 1037, 1749, 1833
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 3 cut(s) 84, 855, 1025
EcoICRI GAGCTC 1 cut(s) 1601
EcoRI GAATTC 2 cut(s) 94, 439
EcoRII CCWGG 7 cut(s) 307, 944, 1050, 1072, 1132, 1194, 1339
EcoRV GATATC 1 cut(s) 1139
EcoT14I CCWWGG 2 cut(s) 1255, 1877
EcoT38I GRGCYC 1 cut(s) 1603
ErhI CCWWGG 2 cut(s) 1255, 1877
Esp3I CGTCTC 2 cut(s) 143, 276
FaeI CATG 8 cut(s) 661, 716, 731, 841, 1159, 1596, 1630, 1887
FaqI GGGAC 4 cut(s) 832, 1115, 1182, 1901
FatI CATG 8 cut(s) 657, 712, 727, 837, 1155, 1592, 1626, 1883
FauNDI CATATG 1 cut(s) 1173
FblI GTMKAC 1 cut(s) 663
FokI GGATG 7 cut(s) 395, 617, 626, 943, 1280, 1291, 1331
FriOI GRGCYC 1 cut(s) 1603
FspBI CTAG 4 cut(s) 231, 393, 1256, 1766
GsuI CTGGAG 2 cut(s) 1178, 1589
HaeIII GGCC 8 cut(s) 90, 105, 176, 836, 1024, 1363, 1494, 1930
HapII CCGG 5 cut(s) 85, 91, 218, 1146, 1891
HgaI GACGC 3 cut(s) 65, 125, 188
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 8 cut(s) 661, 716, 731, 841, 1159, 1596, 1630, 1887
HpaII CCGG 5 cut(s) 85, 91, 218, 1146, 1891
HphI GGTGA 8 cut(s) 118, 154, 299, 356, 383, 470, 743, 749
Hpy166II GTNNAC 4 cut(s) 664, 746, 1013, 1203
Hpy188III TCNNGA 8 cut(s) 281, 1084, 1453, 1606, 1627, 1683, 1964, 1971
Hpy8I GTNNAC 4 cut(s) 664, 746, 1013, 1203
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 6 cut(s) 20, 129, 373, 461, 1069, 1471
HpyCH4III ACNGT 5 cut(s) 255, 287, 697, 1189, 1369
HpyCH4IV ACGT 4 cut(s) 45, 548, 1183, 1351
HpyF3I CTNAG 6 cut(s) 140, 528, 1007, 1284, 1580, 1716
HpySE526I ACGT 4 cut(s) 45, 548, 1183, 1351
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 8 cut(s) 661, 716, 731, 841, 1159, 1596, 1630, 1887
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 2 cut(s) 952, 1629
LguI GCTCTTC 1 cut(s) 1965
LmnI GCTCC 2 cut(s) 1345, 1598
Lsp1109I GCAGC 5 cut(s) 1118, 1390, 1576, 1964, 1967
LweI GCATC 4 cut(s) 418, 921, 1133, 1373
MaeI CTAG 4 cut(s) 231, 393, 1256, 1766
MaeII ACGT 4 cut(s) 45, 548, 1183, 1351
MaeIII GTNAC 4 cut(s) 226, 271, 537, 1768
MalI GATC 2 cut(s) 954, 1631
MboI GATC 2 cut(s) 952, 1629
MhlI GDGCHC 3 cut(s) 1015, 1603, 1836
MlyI GAGTC 3 cut(s) 398, 1742, 1765
MmeI TCCRAC 1 cut(s) 716
MroXI GAANNNNTTC 1 cut(s) 1448
MseI TTAA 5 cut(s) 35, 453, 890, 995, 1038
MslI CAYNNNNRTG 2 cut(s) 519, 1018
MspA1I CMGCKG 4 cut(s) 173, 1497, 1539, 1945
MspCI CTTAAG 1 cut(s) 34
MspI CCGG 5 cut(s) 85, 91, 218, 1146, 1891
MspR9I CCNGG 9 cut(s) 85, 86, 309, 946, 1052, 1074, 1134, 1196, 1341
MvaI CCWGG 7 cut(s) 309, 946, 1052, 1074, 1134, 1196, 1341
MvnI CGCG 1 cut(s) 173
NciI CCSGG 2 cut(s) 85, 86
NdeI CATATG 1 cut(s) 1173
NdeII GATC 2 cut(s) 952, 1629
NlaIII CATG 8 cut(s) 661, 716, 731, 841, 1159, 1596, 1630, 1887
NlaIV GGNNCC 6 cut(s) 30, 89, 1057, 1347, 1710, 1889
NmeAIII GCCGAG 1 cut(s) 1912
NmuCI GTSAC 2 cut(s) 271, 1768
NspI RCATGY 1 cut(s) 716
NspV TTCGAA 2 cut(s) 437, 443
OliI CACNNNNGTG 1 cut(s) 1018
PaeR7I CTCGAG 1 cut(s) 855
PagI TCATGA 1 cut(s) 1626
PasI CCCWGGG 1 cut(s) 1051
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 1 cut(s) 1965
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PdmI GAANNNNTTC 1 cut(s) 1448
PfeI GAWTC 7 cut(s) 277, 304, 784, 1576, 1748, 1802, 1967
PfoI TCCNGGA 2 cut(s) 1072, 1194
PinAI ACCGGT 1 cut(s) 1145
PleI GAGTC 3 cut(s) 397, 1741, 1765
PpsI GAGTC 3 cut(s) 397, 1741, 1765
Ppu21I YACGTR 2 cut(s) 1184, 1352
PscI ACATGT 1 cut(s) 712
PshAI GACNNNNGTC 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 1603
Psp6I CCWGG 7 cut(s) 307, 944, 1050, 1072, 1132, 1194, 1339
PspGI CCWGG 7 cut(s) 307, 944, 1050, 1072, 1132, 1194, 1339
PspN4I GGNNCC 6 cut(s) 30, 89, 1057, 1347, 1710, 1889
PspPI GGNCC 5 cut(s) 88, 1023, 1337, 1362, 1888
PspXI VCTCGAGB 1 cut(s) 855
PsrI GAACNNNNNNTAC 2 cut(s) 708, 740
PvuII CAGCTG 1 cut(s) 1945
RsaI GTAC 6 cut(s) 745, 876, 1186, 1204, 1463, 1696
RsaNI GTAC 6 cut(s) 744, 875, 1185, 1203, 1462, 1695
RseI CAYNNNNRTG 2 cut(s) 519, 1018
SacI GAGCTC 1 cut(s) 1603
SacII CCGCGG 1 cut(s) 174
SapI GCTCTTC 1 cut(s) 1965
SaqAI TTAA 5 cut(s) 35, 453, 890, 995, 1038
Sau3AI GATC 2 cut(s) 952, 1629
Sau96I GGNCC 5 cut(s) 88, 1023, 1337, 1362, 1888
ScaI AGTACT 1 cut(s) 1463
SchI GAGTC 3 cut(s) 398, 1742, 1765
ScrFI CCNGG 9 cut(s) 85, 86, 309, 946, 1052, 1074, 1134, 1196, 1341
SduI GDGCHC 3 cut(s) 1015, 1603, 1836
SfaNI GCATC 4 cut(s) 418, 921, 1133, 1373
SfcI CTRYAG 1 cut(s) 1389
Sfr274I CTCGAG 1 cut(s) 855
Sfr303I CCGCGG 1 cut(s) 174
SfuI TTCGAA 2 cut(s) 437, 443
SgrBI CCGCGG 1 cut(s) 174
SinI GGWCC 2 cut(s) 1337, 1888
SlaI CTCGAG 1 cut(s) 855
SmaI CCCGGG 1 cut(s) 86
SmiMI CAYNNNNRTG 2 cut(s) 519, 1018
SmlI CTYRAG 2 cut(s) 34, 855
SmoI CTYRAG 2 cut(s) 34, 855
SnaBI TACGTA 1 cut(s) 1184
SpeI ACTAGT 1 cut(s) 1765
SspI AATATT 1 cut(s) 894
SspMI CTAG 4 cut(s) 231, 393, 1256, 1766
SstI GAGCTC 1 cut(s) 1603
StyD4I CCNGG 9 cut(s) 83, 84, 307, 944, 1050, 1072, 1132, 1194, 1339
StyI CCWWGG 2 cut(s) 1255, 1877
TaaI ACNGT 5 cut(s) 255, 287, 697, 1189, 1369
TaiI ACGT 4 cut(s) 48, 551, 1186, 1354
TaqI TCGA 8 cut(s) 280, 397, 437, 443, 856, 1092, 1209, 1531
TaqII GACCGA 1 cut(s) 1325
TatI WGTACW 3 cut(s) 874, 1202, 1461
TauI GCSGC 6 cut(s) 108, 147, 170, 173, 176, 1497
TfiI GAWTC 7 cut(s) 277, 304, 784, 1576, 1748, 1802, 1967
Tru1I TTAA 5 cut(s) 35, 453, 890, 995, 1038
Tru9I TTAA 5 cut(s) 35, 453, 890, 995, 1038
TscAI CASTG 5 cut(s) 753, 1015, 1020, 1108, 1116
TseFI GTSAC 2 cut(s) 271, 1768
TseI GCWGC 5 cut(s) 1106, 1403, 1589, 1952, 1955
Tsp45I GTSAC 2 cut(s) 271, 1768
TspDTI ATGAA 7 cut(s) 716, 773, 801, 1172, 1190, 1794, 1854
TspGWI ACGGA 1 cut(s) 1622
TspMI CCCGGG 1 cut(s) 84
TspRI CASTG 5 cut(s) 753, 1015, 1020, 1108, 1116
Vha464I CTTAAG 1 cut(s) 34
VneI GTGCAC 1 cut(s) 1011
VpaK11BI GGWCC 2 cut(s) 1337, 1888
XapI RAATTY 3 cut(s) 94, 439, 1088
XceI RCATGY 1 cut(s) 716
XcmI CCANNNNNNNNNTGG 1 cut(s) 1356
XhoI CTCGAG 1 cut(s) 855
XmaI CCCGGG 1 cut(s) 84
XmaJI CCTAGG 1 cut(s) 1255
XmiI GTMKAC 1 cut(s) 663
XmnI GAANNNNTTC 1 cut(s) 1448
XspI CTAG 4 cut(s) 231, 393, 1256, 1766
ZrmI AGTACT 1 cut(s) 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.