Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0010258

Physical Location & Seq

Genomic Coordinates Forward (+)

39884998 .. 39890120

Gene Structure 5123 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1314700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGGCTCACCGTCTGATGGGATATTTGGTTCCTCTCAATGCCAAGCTAGAAGAAGACACTTCACTCCCCAAAATGCCCCTCTCCCGAGGCCCGAATTACATCGGCCGCAACACCACCGCTTCCCTCTCCGACAAACGACTCAGCCGGAAGCACCTCGCTCTCACTATCGATGCTAGTGATTCCGCCGTTTTGGTCGTGGAGGGGACAAATCCCATTGTTGTGAAATCCGGGAGTAACAGGAAGAAATTGTTTCCGAAAGAAAATGCAACGATACGCAATGGTGATGTTATAGAGCTGATTCCGGGGCATTTTCTTTTCGAGTATAAGACATTGTCTGATAATGAAAATGGAAATAATGTGCGTGTTGTCTGTGACCATAAGAGGGGATCAAATGATGGTGGAAAGGCTGTTGAAGTTGAAGAGTTGACTCGAAAGAGGCCACGTCAACTAGTGTCTCAAGATAAAGGTTCTGAGAGGAGTTTTAAGGCTGAGGTGGAAGGAAGCAGCTCGGGTGCTGATAGAGACGGTGGCAGTGTGGAGACCATACGCCATTTTCGTGTTCCTAATGATAAATTACCACAGACCTTTCGGCTTTTGAATGTGCAGGGACTCCCCCCATGGGCAAATACCTCTTGTGTTTCTGTAAGGGATTTGATTCAGGGGGACATTCTCGTTGCTATCCTTTCCAATTACATGGTAGACATTGATTGGTTGATCCCTGCATGCCCGTCAATTGCCAAAGTTCCACATGTGTTGGTTATTCATGGCGAGGGTGATGGTACACTGGATTACATGAAGAGAAAGAAGCCTGCGAACTGGATTCTACACAAACCACCATTGCCCATTTCCTTTGGTACACACCATTCAAAGGCCATGATTCTTGTTTATCCTCGAGGAGTGAGAATCATTGTACATACAGCAAACTATATACATGTTGACTGGAACAACAAAAGCCAAGGTTTGTGGATGCAAGACTTTCCCTGGAAAGATCAAAATAATCCAAGCAAAGGATGTGGATTCGAAAGTGACTTAATCGATTATCTCAGTGCACTGAAGTGGCCTGAGTTTTCAGTTAATTTTCCTAATCTGGGTAGCTTCAAGATCAATCCGTCATTTTTCAAGAAGTTTGATTACAGCGATGCAGCAGTGAGGCTAATTGCATCTGTCCCTGGATATCATACGGGTAACAACATGAAAAAGTGGGGGCATATGAAGCTTCGCACTGTTCTTCAGGAGTGTATTTTTGATAAAGAGTTTCGGAAATCTCCTCTTGCTTATCAGTTCTCCTCCCTGGGTTCTTTGGACGAGAAGTGGATGGCTGAGTTTGCGTCTTCAATGTCATCAGGCTTATCGGAAGATAGAACTCCTCTTGGTCCTGGGGAGCCACTCATAATATGGCCTACAGTAGAAGATGTCAGATGTTCTTTAGAGGGCTATGCAGCTGGAAATGCCATTCCTAGTCCACTAAAGAATGTGGAGAAAGACTTCTTGAAAAAGTACTGGGCTAAATGGAGAGCAAGCCATACCGGCCGCTGTCGTGCAATGCCACATATAAAAACATTCGCGCGTTATAATGGTCAGAAACTTGCCTGGTTTTTGCTAACTTCAGCAAATCTTAGCAAGGCTGCCTGGGGAGCTCTCCAGAAGAACAACTCACAGTTGATGATCCGTTCTTATGAGCTGGGAATTCTATTTCTACCACGTAAAACATGCAGTTTTGGGTTTTCTTGTACGAGTAATGGGGTTCCATCCAAGGATAAATCTGGAAGGTCAGAAACTTCTGAAGCATCGAGAACAACACTCGTGACTCTGAACTGGCAAGGAAACAGGAATACAGATTCATCATCTGAAGTAATTAAATTGCCTGTGCCTTATGAACTCCCTCCTCAGCCATACACCGCGGAAGATGTGCCTTGGTCTTGGGACCGGCGGTATAACAAGAAAGACGTTTACGGTCAAGTTTGGCCGCGACATGTTCAGCTGTATGCTGCTCAGGACTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000012 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008081 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016787 GO:0016788 GO:0017005 GO:0033554 GO:0034641 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051716 GO:0070259 GO:0071704 GO:0071944 GO:0090304 GO:0097159 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FHA Tyr-DNA_phospho

Protein Analysis

668

Amino Acids

75.0

Weight (kDa)

9.32

Isoelectric Point (pI)

39.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tyr-DNA_phospho	PF06087	196 - 633	2.1e-100	Tyrosyl-DNA phosphodiesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0010258)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15170
fragaria_vesca	FvH4_3g02650 FvH4_3g02650
malus_domestica	MD10G1314700.v1.1
prunus_persica	Prupe.4G026100_v2.0.a1 Prupe.4G026100_v2.0.a1
pyrus_communis	pycom10g26680
rosa_chinensis	RchiOBHm_Chr5g0003961
rosa_laevigata	RLG00000031190 RLG00000031192
rosa_multiflora	Rmu_sc0000547.1_g000008
rosa_roxburghii	Rroxscaffold_1G00072010
rosa_rugosa	Rorug04G0408200.1 Rorug04G0408300.1 Rorug04G0408400 Rorug04G0408500
rosa_samantha	Rh5AG036200 Rh5BG035100 Rh5CG038800 Rh5DG034700
rosa_wichuraiana	Rw5G003300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1572
AbsI	CCTCGAGG	1 cut(s)	891
AccI	GTMKAC	1 cut(s)	699
AccII	CGCG	4 cut(s)	1565, 1567, 1901, 1969
AciI	CCGC	8 cut(s)	106, 117, 183, 1531, 1899, 1901, 1930, 1967
AclWI	GGATC	3 cut(s)	394, 709, 1660
AcoI	YGGCCR	3 cut(s)	103, 1528, 1964
AcsI	RAATTY	1 cut(s)	1686
AcuI	CTGAAG	5 cut(s)	1073, 1214, 1590, 1803, 1869
AfaI	GTAC	5 cut(s)	781, 856, 912, 1499, 1732
AfiI	CCNNNNNNNGG	6 cut(s)	16, 382, 619, 868, 1007, 1088
AflIII	ACRYGT	3 cut(s)	748, 931, 1972
AgsI	TTSAA	8 cut(s)	413, 419, 598, 867, 1099, 1120, 1335, 1492
AhlI	ACTAGT	1 cut(s)	448
AjiI	CACGTC	1 cut(s)	443
AjnI	CCWGG	6 cut(s)	980, 1168, 1290, 1375, 1589, 1628
AleI	CACNNNNGTG	1 cut(s)	1054
AloI	GAACNNNNNNTCC	2 cut(s)	12, 44
AluBI	AGCT	9 cut(s)	46, 295, 507, 1095, 1216, 1442, 1637, 1681, 1981
AluI	AGCT	9 cut(s)	46, 295, 507, 1095, 1216, 1442, 1637, 1681, 1981
Alw21I	GWGCWC	2 cut(s)	1051, 1639
Alw26I	GTCTC	3 cut(s)	459, 516, 533
Alw44I	GTGCAC	1 cut(s)	1047
AlwI	GGATC	3 cut(s)	394, 709, 1660
Ama87I	CYCGRG	3 cut(s)	84, 508, 891
AoxI	GGCC	8 cut(s)	88, 103, 437, 870, 1058, 1397, 1528, 1964
ApaLI	GTGCAC	1 cut(s)	1047
ApeKI	GCWGC	5 cut(s)	504, 1142, 1439, 1625, 1988
ApoI	RAATTY	1 cut(s)	1686
Asp700I	GAANNNNTTC	1 cut(s)	1484
AspLEI	GCGC	1 cut(s)	1567
AspS9I	GGNCC	3 cut(s)	89, 1373, 1924
AsuC2I	CCSGG	2 cut(s)	229, 303
AsuHPI	GGTGA	2 cut(s)	293, 785
AsuII	TTCGAA	1 cut(s)	1020
AvaI	CYCGRG	3 cut(s)	84, 508, 891
AvaII	GGWCC	2 cut(s)	1373, 1924
BaeGI	GKGCMC	1 cut(s)	1051
BanII	GRGCYC	1 cut(s)	1639
BarI	GAAGNNNNNNTAC	2 cut(s)	1115, 1147
BauI	CACGAG	2 cut(s)	1802, 1999
BbsI	GAAGAC	2 cut(s)	60, 1323
Bbv12I	GWGCWC	2 cut(s)	1051, 1639
BbvCI	CCTCAGC	2 cut(s)	489, 1887
BbvI	GCAGC	5 cut(s)	516, 1154, 1451, 1612, 1975
BccI	CCATC	5 cut(s)	10, 389, 770, 1309, 1756
BceAI	ACGGC	1 cut(s)	170
BciT130I	CCWGG	6 cut(s)	982, 1170, 1292, 1377, 1591, 1630
BcnI	CCSGG	2 cut(s)	229, 303
BcoDI	GTCTC	3 cut(s)	459, 516, 533
BcuI	ACTAGT	1 cut(s)	448
BfaI	CTAG	4 cut(s)	47, 174, 449, 1458
BfmI	CTRYAG	1 cut(s)	1401
BglI	GCCNNNNNGGC	1 cut(s)	1527
BisI	GCNGC	8 cut(s)	106, 505, 1143, 1440, 1531, 1626, 1967, 1989
BlsI	GCNGC	8 cut(s)	107, 506, 1144, 1441, 1532, 1627, 1968, 1990
BmcAI	AGTACT	1 cut(s)	1499
Bme1390I	CCNGG	8 cut(s)	229, 303, 982, 1170, 1292, 1377, 1591, 1630
Bme18I	GGWCC	2 cut(s)	1373, 1924
BmeT110I	CYCGRG	3 cut(s)	84, 508, 891
BmgBI	CACGTC	1 cut(s)	443
BmgT120I	GGNCC	3 cut(s)	89, 1373, 1924
BmiI	GGNNCC	4 cut(s)	30, 1383, 1746, 1925
BmrFI	CCNGG	8 cut(s)	229, 303, 982, 1170, 1292, 1377, 1591, 1630
BmrI	ACTGGG	1 cut(s)	1510
BmsI	GCATC	5 cut(s)	160, 957, 1129, 1169, 1796
BmuI	ACTGGG	1 cut(s)	1510
BpiI	GAAGAC	2 cut(s)	60, 1323
BpmI	CTGGAG	1 cut(s)	1625
Bpu10I	CCTNAGC	3 cut(s)	489, 1887, 1992
Bpu14I	TTCGAA	1 cut(s)	1020
BpuEI	CTTGAG	1 cut(s)	441
BpuMI	CCSGG	2 cut(s)	229, 303
Bsa29I	ATCGAT	2 cut(s)	168, 1035
BsaAI	YACGTR	1 cut(s)	1703
BsaI	GGTCTC	1 cut(s)	533
Bsc4I	CCNNNNNNNGG	6 cut(s)	16, 382, 619, 868, 1007, 1088
Bse118I	RCCGGY	2 cut(s)	1526, 1926
Bse1I	ACTGG	5 cut(s)	789, 821, 944, 1505, 1820
Bse3DI	GCAATG	3 cut(s)	283, 836, 1548
BseBI	CCWGG	6 cut(s)	982, 1170, 1292, 1377, 1591, 1630
BseCI	ATCGAT	2 cut(s)	168, 1035
BseGI	GGATG	4 cut(s)	972, 1016, 1320, 1748
BseLI	CCNNNNNNNGG	6 cut(s)	16, 382, 619, 868, 1007, 1088
BseMI	GCAATG	3 cut(s)	283, 836, 1548
BseMII	CTCAG	7 cut(s)	154, 462, 480, 1053, 1057, 1311, 1901
BseNI	ACTGG	5 cut(s)	789, 821, 944, 1505, 1820
BseRI	GAGGAG	6 cut(s)	490, 909, 1257, 1276, 1356, 1875
BseSI	GKGCMC	1 cut(s)	1051
BseX3I	CGGCCG	2 cut(s)	103, 1528
BseXI	GCAGC	5 cut(s)	516, 1154, 1451, 1612, 1975
BseYI	CCCAGC	1 cut(s)	1681
BsgI	GTGCAG	1 cut(s)	623
Bsh1236I	CGCG	4 cut(s)	1565, 1567, 1901, 1969
Bsh1285I	CGRYCG	2 cut(s)	106, 1531
BshFI	GGCC	8 cut(s)	90, 105, 439, 872, 1060, 1399, 1530, 1966
BshVI	ATCGAT	2 cut(s)	168, 1035
BsiEI	CGRYCG	2 cut(s)	106, 1531
BsiHKAI	GWGCWC	2 cut(s)	1051, 1639
BsiHKCI	CYCGRG	3 cut(s)	84, 508, 891
BsiSI	CCGG	5 cut(s)	145, 228, 302, 1527, 1927
BslFI	GGGAC	5 cut(s)	217, 621, 677, 1151, 1937
BslI	CCNNNNNNNGG	6 cut(s)	16, 382, 619, 868, 1007, 1088
BsmAI	GTCTC	3 cut(s)	459, 516, 533
BsmBI	CGTCTC	1 cut(s)	516
BsmFI	GGGAC	5 cut(s)	217, 621, 677, 1151, 1937
BsnI	GGCC	8 cut(s)	90, 105, 439, 872, 1060, 1399, 1530, 1966
Bso31I	GGTCTC	1 cut(s)	533
BsoBI	CYCGRG	3 cut(s)	84, 508, 891
Bsp119I	TTCGAA	1 cut(s)	1020
Bsp1286I	GDGCHC	2 cut(s)	1051, 1639
Bsp1407I	TGTACA	1 cut(s)	910
Bsp143I	GATC	5 cut(s)	386, 714, 988, 1101, 1665
Bsp19I	CCATGG	1 cut(s)	617
BspACI	CCGC	8 cut(s)	106, 117, 183, 1531, 1899, 1901, 1930, 1967
BspANI	GGCC	8 cut(s)	90, 105, 439, 872, 1060, 1399, 1530, 1966
BspCNI	CTCAG	7 cut(s)	153, 463, 481, 1054, 1056, 1312, 1900
BspDI	ATCGAT	2 cut(s)	168, 1035
BspFNI	CGCG	4 cut(s)	1565, 1567, 1901, 1969
BspLI	GGNNCC	4 cut(s)	30, 1383, 1746, 1925
BspPI	GGATC	3 cut(s)	394, 709, 1660
BspT104I	TTCGAA	1 cut(s)	1020
BspTNI	GGTCTC	1 cut(s)	533
BsrDI	GCAATG	3 cut(s)	283, 836, 1548
BsrFI	RCCGGY	2 cut(s)	1526, 1926
BsrGI	TGTACA	1 cut(s)	910
BsrI	ACTGG	5 cut(s)	789, 821, 944, 1505, 1820
BssAI	RCCGGY	2 cut(s)	1526, 1926
BssMI	GATC	5 cut(s)	386, 714, 988, 1101, 1665
BssSI	CACGAG	2 cut(s)	1802, 1999
BssT1I	CCWWGG	4 cut(s)	617, 955, 1752, 1913
Bst2BI	CACGAG	2 cut(s)	1802, 1999
Bst2UI	CCWGG	6 cut(s)	982, 1170, 1292, 1377, 1591, 1630
Bst4CI	ACNGT	6 cut(s)	11, 527, 1225, 1405, 1659, 1955
Bst6I	CTCTTC	2 cut(s)	414, 791
BstAUI	TGTACA	1 cut(s)	910
BstBAI	YACGTR	1 cut(s)	1703
BstBI	TTCGAA	1 cut(s)	1020
BstC8I	GCNNGC	3 cut(s)	724, 810, 1519
BstDEI	CTNAG	9 cut(s)	140, 471, 489, 1043, 1062, 1320, 1616, 1887, 1992
BstDSI	CCRYGG	2 cut(s)	617, 1899
BstF5I	GGATG	4 cut(s)	972, 1016, 1320, 1748
BstFNI	CGCG	4 cut(s)	1565, 1567, 1901, 1969
BstHHI	GCGC	1 cut(s)	1567
BstKTI	GATC	5 cut(s)	389, 717, 991, 1104, 1668
BstMAI	GTCTC	3 cut(s)	459, 516, 533
BstMBI	GATC	5 cut(s)	386, 714, 988, 1101, 1665
BstMCI	CGRYCG	2 cut(s)	106, 1531
BstMWI	GCNNNNNNNGC	5 cut(s)	1213, 1325, 1448, 1527, 1634
BstNI	CCWGG	6 cut(s)	982, 1170, 1292, 1377, 1591, 1630
BstNSI	RCATGY	5 cut(s)	726, 752, 935, 1713, 1976
BstSCI	CCNGG	8 cut(s)	227, 301, 980, 1168, 1290, 1375, 1589, 1628
BstSFI	CTRYAG	1 cut(s)	1401
BstSLI	GKGCMC	1 cut(s)	1051
BstUI	CGCG	4 cut(s)	1565, 1567, 1901, 1969
BstV1I	GCAGC	5 cut(s)	516, 1154, 1451, 1612, 1975
BstV2I	GAAGAC	2 cut(s)	60, 1323
BstXI	CCANNNNNNTGG	1 cut(s)	694
BstZI	CGGCCG	2 cut(s)	103, 1528
Bsu15I	ATCGAT	2 cut(s)	168, 1035
BsuRI	GGCC	8 cut(s)	90, 105, 439, 872, 1060, 1399, 1530, 1966
BsuTUI	ATCGAT	2 cut(s)	168, 1035
BtgI	CCRYGG	2 cut(s)	617, 1899
BtgZI	GCGATG	1 cut(s)	1152
BtrI	CACGTC	1 cut(s)	443
BtsCI	GGATG	4 cut(s)	972, 1016, 1320, 1748
BtsI	GCAGTG	2 cut(s)	538, 1152
BtsIMutI	CAGTG	6 cut(s)	538, 782, 1049, 1051, 1152, 1221
Cac8I	GCNNGC	3 cut(s)	724, 810, 1519
CfoI	GCGC	1 cut(s)	1567
Cfr10I	RCCGGY	2 cut(s)	1526, 1926
Cfr13I	GGNCC	3 cut(s)	89, 1373, 1924
Cfr42I	CCGCGG	1 cut(s)	1902
ClaI	ATCGAT	2 cut(s)	168, 1035
CseI	GACGC	1 cut(s)	1317
Csp6I	GTAC	5 cut(s)	780, 855, 911, 1498, 1731
CspCI	CAANNNNNGTGG	6 cut(s)	735, 770, 944, 979, 994, 1029
CviQI	GTAC	5 cut(s)	780, 855, 911, 1498, 1731
DdeI	CTNAG	9 cut(s)	140, 471, 489, 1043, 1062, 1320, 1616, 1887, 1992
DpnI	GATC	5 cut(s)	388, 716, 990, 1103, 1667
DpnII	GATC	5 cut(s)	386, 714, 988, 1101, 1665
EaeI	YGGCCR	3 cut(s)	103, 1528, 1964
EagI	CGGCCG	2 cut(s)	103, 1528
Eam1104I	CTCTTC	2 cut(s)	414, 791
EarI	CTCTTC	2 cut(s)	414, 791
EciI	GGCGGA	1 cut(s)	172
Ecl136II	GAGCTC	1 cut(s)	1637
EclXI	CGGCCG	2 cut(s)	103, 1528
Eco130I	CCWWGG	4 cut(s)	617, 955, 1752, 1913
Eco24I	GRGCYC	1 cut(s)	1639
Eco31I	GGTCTC	1 cut(s)	533
Eco32I	GATATC	1 cut(s)	1175
Eco47I	GGWCC	2 cut(s)	1373, 1924
Eco52I	CGGCCG	2 cut(s)	103, 1528
Eco53kI	GAGCTC	1 cut(s)	1637
Eco57I	CTGAAG	5 cut(s)	1073, 1214, 1590, 1803, 1869
Eco88I	CYCGRG	3 cut(s)	84, 508, 891
EcoICRI	GAGCTC	1 cut(s)	1637
EcoRI	GAATTC	1 cut(s)	1686
EcoRII	CCWGG	6 cut(s)	980, 1168, 1290, 1375, 1589, 1628
EcoRV	GATATC	1 cut(s)	1175
EcoT14I	CCWWGG	4 cut(s)	617, 955, 1752, 1913
EcoT38I	GRGCYC	1 cut(s)	1639
ErhI	CCWWGG	4 cut(s)	617, 955, 1752, 1913
Esp3I	CGTCTC	1 cut(s)	516
FaqI	GGGAC	5 cut(s)	217, 621, 677, 1151, 1937
FauNDI	CATATG	1 cut(s)	1209
FblI	GTMKAC	1 cut(s)	699
Fnu4HI	GCNGC	8 cut(s)	106, 505, 1143, 1440, 1531, 1626, 1967, 1989
FokI	GGATG	4 cut(s)	979, 1023, 1327, 1735
FriOI	GRGCYC	1 cut(s)	1639
Fsp4HI	GCNGC	8 cut(s)	106, 505, 1143, 1440, 1531, 1626, 1967, 1989
FspBI	CTAG	4 cut(s)	47, 174, 449, 1458
GlaI	GCGC	1 cut(s)	1566
GluI	GCNGC	8 cut(s)	106, 505, 1143, 1440, 1531, 1626, 1967, 1989
GsaI	CCCAGC	1 cut(s)	1685
GsuI	CTGGAG	1 cut(s)	1625
HaeIII	GGCC	8 cut(s)	90, 105, 439, 872, 1060, 1399, 1530, 1966
HapII	CCGG	5 cut(s)	145, 228, 302, 1527, 1927
HgaI	GACGC	1 cut(s)	1317
HhaI	GCGC	1 cut(s)	1567
Hin6I	GCGC	1 cut(s)	1565
HinP1I	GCGC	1 cut(s)	1565
HincII	GTYRAC	3 cut(s)	426, 446, 937
HindII	GTYRAC	3 cut(s)	426, 446, 937
HindIII	AAGCTT	1 cut(s)	1214
HpaII	CCGG	5 cut(s)	145, 228, 302, 1527, 1927
HphI	GGTGA	2 cut(s)	293, 785
Hpy166II	GTNNAC	9 cut(s)	426, 446, 700, 782, 857, 937, 1049, 1463, 1951
Hpy8I	GTNNAC	9 cut(s)	426, 446, 700, 782, 857, 937, 1049, 1463, 1951
HpyAV	CCTTC	2 cut(s)	491, 1761
HpyCH4III	ACNGT	6 cut(s)	11, 527, 1225, 1405, 1659, 1955
HpyCH4IV	ACGT	3 cut(s)	442, 1702, 1947
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1213, 1325, 1448, 1527, 1634
HpyF3I	CTNAG	9 cut(s)	140, 471, 489, 1043, 1062, 1320, 1616, 1887, 1992
HpySE526I	ACGT	3 cut(s)	442, 1702, 1947
HspAI	GCGC	1 cut(s)	1565
KspI	CCGCGG	1 cut(s)	1902
Kzo9I	GATC	5 cut(s)	386, 714, 988, 1101, 1665
LmnI	GCTCC	2 cut(s)	1381, 1634
Lsp1109I	GCAGC	5 cut(s)	516, 1154, 1451, 1612, 1975
LweI	GCATC	5 cut(s)	160, 957, 1129, 1169, 1796
MaeI	CTAG	4 cut(s)	47, 174, 449, 1458
MaeII	ACGT	3 cut(s)	442, 1702, 1947
MaeIII	GTNAC	5 cut(s)	233, 371, 1025, 1184, 1804
MalI	GATC	5 cut(s)	388, 716, 990, 1103, 1667
MboI	GATC	5 cut(s)	386, 714, 988, 1101, 1665
MfeI	CAATTG	1 cut(s)	732
MhlI	GDGCHC	2 cut(s)	1051, 1639
MlyI	GAGTC	5 cut(s)	132, 421, 603, 1801, 1991
MmeI	TCCRAC	1 cut(s)	153
MroXI	GAANNNNTTC	1 cut(s)	1484
MseI	TTAA	4 cut(s)	483, 1031, 1074, 1857
MslI	CAYNNNNRTG	3 cut(s)	218, 555, 1054
MspA1I	CMGCKG	4 cut(s)	1442, 1533, 1901, 1981
MspI	CCGG	5 cut(s)	145, 228, 302, 1527, 1927
MspR9I	CCNGG	8 cut(s)	229, 303, 982, 1170, 1292, 1377, 1591, 1630
MunI	CAATTG	1 cut(s)	732
MvaI	CCWGG	6 cut(s)	982, 1170, 1292, 1377, 1591, 1630
MvnI	CGCG	4 cut(s)	1565, 1567, 1901, 1969
MwoI	GCNNNNNNNGC	5 cut(s)	1213, 1325, 1448, 1527, 1634
NciI	CCSGG	2 cut(s)	229, 303
NcoI	CCATGG	1 cut(s)	617
NdeI	CATATG	1 cut(s)	1209
NdeII	GATC	5 cut(s)	386, 714, 988, 1101, 1665
NlaIV	GGNNCC	4 cut(s)	30, 1383, 1746, 1925
NmuCI	GTSAC	3 cut(s)	371, 1025, 1804
NspI	RCATGY	5 cut(s)	726, 752, 935, 1713, 1976
NspV	TTCGAA	1 cut(s)	1020
OliI	CACNNNNGTG	1 cut(s)	1054
PaeI	GCATGC	1 cut(s)	726
PaeR7I	CTCGAG	1 cut(s)	891
PasI	CCCWGGG	1 cut(s)	1291
PciI	ACATGT	3 cut(s)	748, 931, 1972
PcsI	WCGNNNNNNNCGW	1 cut(s)	553
PdmI	GAANNNNTTC	1 cut(s)	1484
PfeI	GAWTC	8 cut(s)	179, 298, 655, 820, 877, 903, 1017, 1838
PflFI	GACNNNGTC	1 cut(s)	331
PfoI	TCCNGGA	1 cut(s)	227
PkrI	GCNGC	8 cut(s)	107, 506, 1144, 1441, 1532, 1627, 1968, 1990
PleI	GAGTC	5 cut(s)	132, 421, 603, 1801, 1991
PpsI	GAGTC	5 cut(s)	132, 421, 603, 1801, 1991
Ppu21I	YACGTR	1 cut(s)	1703
PscI	ACATGT	3 cut(s)	748, 931, 1972
PsiI	TTATAA	1 cut(s)	1572
Psp124BI	GAGCTC	1 cut(s)	1639
Psp6I	CCWGG	6 cut(s)	980, 1168, 1290, 1375, 1589, 1628
PspFI	CCCAGC	1 cut(s)	1681
PspGI	CCWGG	6 cut(s)	980, 1168, 1290, 1375, 1589, 1628
PspN4I	GGNNCC	4 cut(s)	30, 1383, 1746, 1925
PspPI	GGNCC	3 cut(s)	89, 1373, 1924
PspXI	VCTCGAGB	1 cut(s)	891
PsyI	GACNNNGTC	1 cut(s)	331
PvuII	CAGCTG	2 cut(s)	1442, 1981
RsaI	GTAC	5 cut(s)	781, 856, 912, 1499, 1732
RsaNI	GTAC	5 cut(s)	780, 855, 911, 1498, 1731
RseI	CAYNNNNRTG	3 cut(s)	218, 555, 1054
SacI	GAGCTC	1 cut(s)	1639
SacII	CCGCGG	1 cut(s)	1902
SaqAI	TTAA	4 cut(s)	483, 1031, 1074, 1857
SatI	GCNGC	8 cut(s)	106, 505, 1143, 1440, 1531, 1626, 1967, 1989
Sau3AI	GATC	5 cut(s)	386, 714, 988, 1101, 1665
Sau96I	GGNCC	3 cut(s)	89, 1373, 1924
ScaI	AGTACT	1 cut(s)	1499
SchI	GAGTC	5 cut(s)	132, 421, 603, 1801, 1991
ScrFI	CCNGG	8 cut(s)	229, 303, 982, 1170, 1292, 1377, 1591, 1630
SduI	GDGCHC	2 cut(s)	1051, 1639
SfaNI	GCATC	5 cut(s)	160, 957, 1129, 1169, 1796
SfcI	CTRYAG	1 cut(s)	1401
Sfr274I	CTCGAG	1 cut(s)	891
Sfr303I	CCGCGG	1 cut(s)	1902
SfuI	TTCGAA	1 cut(s)	1020
SgrBI	CCGCGG	1 cut(s)	1902
SinI	GGWCC	2 cut(s)	1373, 1924
SlaI	CTCGAG	1 cut(s)	891
SmiMI	CAYNNNNRTG	3 cut(s)	218, 555, 1054
SmlI	CTYRAG	2 cut(s)	456, 891
SmoI	CTYRAG	2 cut(s)	456, 891
SpeI	ACTAGT	1 cut(s)	448
SphI	GCATGC	1 cut(s)	726
SsiI	CCGC	8 cut(s)	106, 117, 183, 1531, 1899, 1901, 1930, 1967
SspMI	CTAG	4 cut(s)	47, 174, 449, 1458
SstI	GAGCTC	1 cut(s)	1639
StyD4I	CCNGG	8 cut(s)	227, 301, 980, 1168, 1290, 1375, 1589, 1628
StyI	CCWWGG	4 cut(s)	617, 955, 1752, 1913
TaaI	ACNGT	6 cut(s)	11, 527, 1225, 1405, 1659, 1955
TaiI	ACGT	3 cut(s)	445, 1705, 1950
TaqI	TCGA	7 cut(s)	168, 318, 430, 892, 1020, 1035, 1790
TatI	WGTACW	2 cut(s)	910, 1497
TauI	GCSGC	3 cut(s)	108, 1533, 1969
TfiI	GAWTC	8 cut(s)	179, 298, 655, 820, 877, 903, 1017, 1838
Tru1I	TTAA	4 cut(s)	483, 1031, 1074, 1857
Tru9I	TTAA	4 cut(s)	483, 1031, 1074, 1857
TscAI	CASTG	6 cut(s)	538, 789, 1051, 1056, 1152, 1228
TseFI	GTSAC	3 cut(s)	371, 1025, 1804
TseI	GCWGC	5 cut(s)	504, 1142, 1439, 1625, 1988
Tsp45I	GTSAC	3 cut(s)	371, 1025, 1804
TspDTI	ATGAA	7 cut(s)	357, 752, 809, 1208, 1226, 1830, 1890
TspGWI	ACGGA	2 cut(s)	1098, 1658
TspRI	CASTG	6 cut(s)	538, 789, 1051, 1056, 1152, 1228
Tth111I	GACNNNGTC	1 cut(s)	331
VneI	GTGCAC	1 cut(s)	1047
VpaK11BI	GGWCC	2 cut(s)	1373, 1924
XapI	RAATTY	1 cut(s)	1686
XceI	RCATGY	5 cut(s)	726, 752, 935, 1713, 1976
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1392
XhoI	CTCGAG	1 cut(s)	891
XmiI	GTMKAC	1 cut(s)	699
XmnI	GAANNNNTTC	1 cut(s)	1484
XspI	CTAG	4 cut(s)	47, 174, 449, 1458
ZrmI	AGTACT	1 cut(s)	1499

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.