Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004429

Physical Location & Seq

Genomic Coordinates Forward (+)

5485203 .. 5493548

Gene Structure 8346 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G16715.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGATTCTCAAAACGGCTTTTTCTCTACCCACACCTACTACTACTCTATTATCTCCCAGCTCTCCTCACCAGCTCAACACTCTGTTCTTCACTCGCCGACGCCGTCGATTAATTTCCCCATCCCGCTTGAACTCAATTTTCTCGCAACGTAGATTCTCTTTCTCTGCTGCTGCGTCAGGAAATAATGTATTTACTTCTCCCGAGACATCCAAGACCTTTGATTTTTCTTCGGAGGAGAAGATATACAAATGGTGGGAGTCGCAAGGCTACTTCAAACCAAATTTTGACCAAGGAGGCAGTCCTTTTGTGATACCGATGCCACCTCCTAATGTTACTGGTTCTCTGCACATGGGCCATGCAATGTTTGTCACTCTTGAGGATATTATGGTTCGATACAATAGGATGAATGGGAGACCAACACTTTGGCTTCCCGGGACTGATCATGCTGGTATTGCCACTCAGTTGGTCGTGGAAAAGATGCTAGCGTCAGAAGGCATTAAGAGAGTGGACTTAGGTAGAGATGAGTTTACCAAAAGAGTCTGGGAATGGAAGGAAAAGTATGGCGGAACCATCACAAATCAGATTAAAAGACTGGGTGCTTCTTGTGATTGGAGTAGAGAGCGCTTTACTCTTGACGAGCAATTGAGTCGAGCTGTGGTCGAAGCTTTTGTGAAGCTTCATGACAAGGGGTTGATATATCAAGGATCTTACATGGTCAACTGGTCCCCAAATTTACAGACTGCTGTTTCAGACTTGGAAGTAGAATATTCGGAGGAACCTGGGTTTCTATACCATATTAAATACCGTGTAGCTGGAAGTCCTGATTTTCTTACTATAGCAACGACTCGTCCAGAGACATTGTTTGGTGACGTGGCCCTTGCCGTGCATCCTGAGGATGATCGTTACTCAAAATATGTTGGTCAGACAGCAATTGTACCTATGACATATGGTCGTCATGTCCCAATTATTGCTGATAAGTATGTTGATAAGGATTTTGGGACTGGTGTTCTGAAGATAAGTCCTGGGCATGATCACAACGATTACCTTCTTGCAAGAAAGCTAGGTCTCCCCATTCTGAATGTGATGAACAAGGATGCAACACTTAATGACGTCGCTGGCTTGTTTTGTGGCCTTGACAGGTTTGAGGTGAGGGAGAAACTATGGGCAGATCTTGAGGAGATAGGTTTAGCTGTTAAGAAGGAGCCTCACACTTTACGAGTTCCAAGATCTCAGCGTGGTGGAGAAGTTATTGAGCCACTTGTAAGCAAACAATGGTTTGTCCACATGGATCCTTTAGCTGAGAAGGCTCTTCTTGCTGTTGAAAACAAAGAACTTACTATCATACCTGAGAGATTTGAGAAGATATACAATCACTGGCTGACAAATATTAAGGATTGGTGCATAAGCCGGCAGCTGTGGTGGGGACATCGCATACCAGTTTGGTATGTGGTTGGAAAGGATTGCGAGGAGGACTACATAGTTGCTAAAAGTGCTGAAGAAGCCCTTGAGAAAGCCCTTGAGAAGTATGGAAAAGATGTTGAAATATATCAGGATCCAGATGTTCTTGACACCTGGTTTTCCAGCTCGTTGTGGCCATTCAGCACTCTTGGCTGGCCTGATGTAGCAGCGAAGGATTTCAATAACTTCTACCCAACAAATATGTTAGAAACAGGGCATGACATACTATTTTTTTGGGTAGCAAGAATGGTTATGATGGGAATAGAATTTACAGGGACCGTTCCATTTTCTCATGTCTACCTTCATGGACTTATAAGAGACTCACAAGGGAGAAAAATGTCAAAATCTCTTGGGAATGTGATTGATCCGCTTGACACAATTAAAGATTTTGGCACTGATGCTCTGAGATTTACGATTGCACTAGGAACTGCTGGTCAGGATCTGAACTTATCTACTGAGAGGTTAACTGCAAATAAAGCATTCACCAACAAACTCTGGAATGCTGGAAAGTTTGTGCTTCATAGTCTGCCTAGTCTAAGTGATACATCTGCTTGGGAGAATTTGCTGGACCTCAAGTTGGACAAAGAGGAAACCCTGCTTAGTTTACCCTTGCCTGAGTGTTGGGCGGTGTCAAAACTCCATATTCTCATTGATTCTGTCACAGCAAGCTATGAGAAGTTGTTCTTTGGGGATGTAGGAAGAGAAACATATGATTTCTTTTGGAGTGACTTTGCTGATTGGTATATTGAAGCCAGCAAATCTCGACTGTATGGATCTGGAGGCAATTCAGTTTCTCTAGCTTCTCAGGCGGTTTTGTTATATGTCTTTGAGAATATACTTAAGCTGTTGCATCCGTTTATGCCGTTTGTAACCGAGGATCTATGGCAGGCACTTCCCTACAGGAAAGAAGCGCTCATTGTGTCTCCATGGCCACAAAATTCACTTCCAAGGAATGTTGAATCAATAAAAAGATTTGAGAATTTACAAGCTCTGACTAGAGCAATTCGAAATGCACGAGCAGAGTATTCAGTGGAACCAGTAAAGCGTATATCTGCCTCAGTTGTTGGAAGTGCAGAGGTCATTGAATATATTTCGAAAGAGAAGGAGGTATTAGCTCTTCTATCCAGGCTTGACTTAAACAACGTCCATTTTTCCAATGCCCCTCCTGGTGATGCAAATCTATCAGTCCACCTTGTAGCCAGTGAAGGACTCGAGGCATATCTACCTCTTGCAGCCATGGTTGATATATCATCTGAAGTCCAACGCATTTCTAAACGTCTCTCCAAGATGCAAACTGAATATGACGCCCTCATAACTCGTCTCAGTTCACCAAAGTTTGTGGAGAAAGCTCCAGAAGAAGTGGTGAGAGGTGTAAAGGAGCAAGTAGAAGAATTAGAAGAGAAGATAAAGTTGACCAAAGCTAGGCTTGATTTTCTCAAGTCTACTACTTCTTTAGTGTCTCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0004812 GO:0004832 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006438 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0022414 GO:0032501 GO:0032502 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

Anticodon_1 Val_tRNA-synt_C tRNA-synt_1

Protein Analysis

974

Amino Acids

110.63

Weight (kDa)

5.96

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1	PF00133	80 - 637	4.1e-152	tRNA synthetases class I (I, L, M and V)
tRNA-synt_1g	PF09334	106 - 255	4e-08	tRNA synthetases class I (M)
tRNA-synt_1_2	PF13603	306 - 366	1.2e-07	Leucyl-tRNA synthetase, editing domain
tRNA-synt_1g	PF09334	399 - 653	8.4e-12	tRNA synthetases class I (M)
Anticodon_1	PF08264	694 - 835	2.7e-38	Anticodon-binding domain of tRNA ligase
Val_tRNA-synt_C	PF10458	901 - 965	1.6e-13	Valyl tRNA synthetase tRNA binding arm

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004429)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16715
fragaria_vesca	FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130 FvH4_7g04130
malus_domestica	MD05G1034800.v1.1 MD09G1264300.v1.1 MD09G1264400.v1.1 MD17G1258800.v1.1 MD17G1259600.v1.1 MD17G1259800.v1.1 MD17G1260100.v1.1
prunus_persica	Prupe.3G161900_v2.0.a1 Prupe.3G162000_v2.0.a1
pyrus_communis	pycom09g17800 pycom17g26100
rosa_chinensis	RchiOBHm_Chr1g0328051
rosa_laevigata	RLG00000029942
rosa_multiflora	Rmu_sc0001829.1_g000010
rosa_roxburghii	Rroxscaffold_4G00321900
rosa_rugosa	Rorug01G0073400
rosa_samantha	Rh1AG090500 Rh1BG072400 Rh1CG087100 Rh1DG093800
rosa_wichuraiana	Rw1G007020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1772
AatII	GACGTC	1 cut(s)	1113
AccI	GTMKAC	2 cut(s)	1755, 2900
AciI	CCGC	5 cut(s)	124, 564, 1826, 2084, 2267
AclWI	GGATC	9 cut(s)	712, 1283, 1296, 1547, 1560, 1817, 1905, 2239, 2343
AcoI	YGGCCR	2 cut(s)	1592, 2387
AcsI	RAATTY	6 cut(s)	280, 730, 1724, 2017, 2395, 2437
AcuI	CTGAAG	3 cut(s)	1031, 1515, 2733
AcyI	GRCGYC	3 cut(s)	100, 1110, 2763
AfaI	GTAC	1 cut(s)	936
AfeI	AGCGCT	2 cut(s)	623, 2370
AfiI	CCNNNNNNNGG	2 cut(s)	2035, 2880
AflII	CTTAAG	1 cut(s)	2297
AgsI	TTSAA	8 cut(s)	130, 274, 1322, 1541, 1639, 2207, 2417, 2543
AhdI	GACNNNNNGTC	1 cut(s)	948
AjiI	CACGTC	1 cut(s)	871
AjnI	CCWGG	5 cut(s)	778, 1021, 1571, 2582, 2623
Alw26I	GTCTC	8 cut(s)	197, 406, 848, 1070, 1770, 2385, 2741, 2783
AlwI	GGATC	9 cut(s)	712, 1283, 1296, 1547, 1560, 1817, 1905, 2239, 2343
AlwNI	CAGNNNCTG	1 cut(s)	1900
Ama87I	CYCGRG	3 cut(s)	200, 431, 2669
Aor51HI	AGCGCT	2 cut(s)	623, 2370
AoxI	GGCC	6 cut(s)	352, 873, 1129, 1592, 1613, 2387
ApeKI	GCWGC	5 cut(s)	167, 170, 1411, 1625, 2690
ApoI	RAATTY	6 cut(s)	280, 730, 1724, 2017, 2395, 2437
ArsI	GACNNNNNNTTYG	2 cut(s)	405, 437
AseI	ATTAAT	1 cut(s)	110
Asp700I	GAANNNNTTC	2 cut(s)	1634, 2138
AspLEI	GCGC	2 cut(s)	624, 2371
AspS9I	GGNCC	5 cut(s)	352, 723, 874, 1734, 2026
AsuC2I	CCSGG	2 cut(s)	432, 433
AsuHPI	GGTGA	7 cut(s)	59, 878, 1159, 1933, 2639, 2778, 2833
AsuII	TTCGAA	2 cut(s)	2464, 2552
AsuNHI	GCTAGC	1 cut(s)	481
AvaI	CYCGRG	3 cut(s)	200, 431, 2669
AvaII	GGWCC	3 cut(s)	723, 1734, 2026
AxyI	CCTNAGG	1 cut(s)	891
BalI	TGGCCA	2 cut(s)	1594, 2389
BamHI	GGATCC	2 cut(s)	1288, 1552
BarI	GAAGNNNNNNTAC	2 cut(s)	2559, 2591
BauI	CACGAG	1 cut(s)	2472
BbvI	GCAGC	5 cut(s)	154, 157, 1423, 1637, 2702
BccI	CCATC	3 cut(s)	127, 578, 1708
BceAI	ACGGC	4 cut(s)	30, 87, 866, 2305
BcgI	CGANNNNNNTGC	2 cut(s)	629, 663
BciT130I	CCWGG	5 cut(s)	780, 1023, 1573, 2584, 2625
BclI	TGATCA	2 cut(s)	439, 1030
BcnI	CCSGG	2 cut(s)	432, 433
BcoDI	GTCTC	8 cut(s)	197, 406, 848, 1070, 1770, 2385, 2741, 2783
BfaI	CTAG	7 cut(s)	482, 1061, 1880, 1989, 2255, 2454, 2880
BfmI	CTRYAG	2 cut(s)	834, 2356
BfoI	RGCGCY	2 cut(s)	625, 2372
BfrI	CTTAAG	1 cut(s)	2297
BglII	AGATCT	2 cut(s)	1168, 1226
BisI	GCNGC	5 cut(s)	168, 171, 1412, 1626, 2691
BlsI	GCNGC	5 cut(s)	169, 172, 1413, 1627, 2692
Bme1390I	CCNGG	7 cut(s)	432, 433, 780, 1023, 1573, 2584, 2625
Bme18I	GGWCC	3 cut(s)	723, 1734, 2026
BmeRI	GACNNNNNGTC	1 cut(s)	948
BmeT110I	CYCGRG	3 cut(s)	200, 431, 2669
BmgBI	CACGTC	1 cut(s)	871
BmgT120I	GGNCC	5 cut(s)	352, 723, 874, 1734, 2026
BmiI	GGNNCC	8 cut(s)	568, 725, 777, 1203, 1290, 1554, 1735, 2493
BmrFI	CCNGG	7 cut(s)	432, 433, 780, 1023, 1573, 2584, 2625
BmrI	ACTGGG	1 cut(s)	602
BmsI	GCATC	8 cut(s)	306, 468, 895, 1084, 1846, 2317, 2620, 2736
BmtI	GCTAGC	1 cut(s)	485
BmuI	ACTGGG	1 cut(s)	602
BpmI	CTGGAG	2 cut(s)	2256, 2793
Bpu14I	TTCGAA	2 cut(s)	2464, 2552
BpuEI	CTTGAG	6 cut(s)	395, 1193, 1526, 1538, 2015, 2879
BpuMI	CCSGG	2 cut(s)	432, 433
BsaBI	GATNNNNATC	1 cut(s)	2634
BsaHI	GRCGYC	3 cut(s)	100, 1110, 2763
BsaI	GGTCTC	2 cut(s)	406, 1070
BsaJI	CCNNGG	8 cut(s)	289, 431, 779, 1022, 2331, 2384, 2405, 2694
Bsc4I	CCNNNNNNNGG	2 cut(s)	2035, 2880
Bse118I	RCCGGY	1 cut(s)	1407
Bse1I	ACTGG	8 cut(s)	340, 597, 725, 1006, 1379, 1436, 2495, 2658
Bse21I	CCTNAGG	1 cut(s)	891
Bse3DI	GCAATG	1 cut(s)	366
Bse8I	GATNNNNATC	1 cut(s)	2634
BseBI	CCWGG	5 cut(s)	780, 1023, 1573, 2584, 2625
BseDI	CCNNGG	8 cut(s)	289, 431, 779, 1022, 2331, 2384, 2405, 2694
BseGI	GGATG	8 cut(s)	119, 206, 408, 886, 901, 1099, 2155, 2308
BseJI	GATNNNNATC	1 cut(s)	2634
BseLI	CCNNNNNNNGG	2 cut(s)	2035, 2880
BseMI	GCAATG	1 cut(s)	366
BseNI	ACTGG	8 cut(s)	340, 597, 725, 1006, 1379, 1436, 2495, 2658
BseRI	GAGGAG	4 cut(s)	54, 248, 1190, 1481
BseXI	GCAGC	5 cut(s)	154, 157, 1423, 1637, 2702
BseYI	CCCAGC	1 cut(s)	56
BsgI	GTGCAG	2 cut(s)	329, 2550
BshFI	GGCC	6 cut(s)	354, 875, 1131, 1594, 1615, 2389
BsiHKCI	CYCGRG	3 cut(s)	200, 431, 2669
BsiSI	CCGG	2 cut(s)	432, 1408
BslFI	GGGAC	6 cut(s)	448, 709, 944, 1012, 1437, 1747
BslI	CCNNNNNNNGG	2 cut(s)	2035, 2880
BsmAI	GTCTC	8 cut(s)	197, 406, 848, 1070, 1770, 2385, 2741, 2783
BsmBI	CGTCTC	2 cut(s)	2741, 2783
BsmFI	GGGAC	6 cut(s)	448, 709, 944, 1012, 1437, 1747
BsmI	GAATGC	2 cut(s)	1937, 1963
BsnI	GGCC	6 cut(s)	354, 875, 1131, 1594, 1615, 2389
Bso31I	GGTCTC	2 cut(s)	406, 1070
BsoBI	CYCGRG	3 cut(s)	200, 431, 2669
Bsp119I	TTCGAA	2 cut(s)	2464, 2552
Bsp19I	CCATGG	2 cut(s)	2384, 2694
BspACI	CCGC	5 cut(s)	124, 564, 1826, 2084, 2267
BspANI	GGCC	6 cut(s)	354, 875, 1131, 1594, 1615, 2389
BspHI	TCATGA	1 cut(s)	679
BspLI	GGNNCC	8 cut(s)	568, 725, 777, 1203, 1290, 1554, 1735, 2493
BspOI	GCTAGC	1 cut(s)	485
BspPI	GGATC	9 cut(s)	712, 1283, 1296, 1547, 1560, 1817, 1905, 2239, 2343
BspQI	GCTCTTC	2 cut(s)	1314, 2580
BspT104I	TTCGAA	2 cut(s)	2464, 2552
BspTI	CTTAAG	1 cut(s)	2297
BspTNI	GGTCTC	2 cut(s)	406, 1070
BsrDI	GCAATG	1 cut(s)	366
BsrFI	RCCGGY	1 cut(s)	1407
BsrI	ACTGG	8 cut(s)	340, 597, 725, 1006, 1379, 1436, 2495, 2658
BssAI	RCCGGY	1 cut(s)	1407
BssECI	CCNNGG	8 cut(s)	289, 431, 779, 1022, 2331, 2384, 2405, 2694
BssNI	GRCGYC	3 cut(s)	100, 1110, 2763
BssSI	CACGAG	1 cut(s)	2472
BssT1I	CCWWGG	4 cut(s)	289, 2384, 2405, 2694
Bst2BI	CACGAG	1 cut(s)	2472
Bst2UI	CCWGG	5 cut(s)	780, 1023, 1573, 2584, 2625
Bst4CI	ACNGT	3 cut(s)	806, 1738, 2226
Bst6I	CTCTTC	4 cut(s)	1314, 2152, 2580, 2850
BstACI	GRCGYC	3 cut(s)	100, 1110, 2763
BstAFI	CTTAAG	1 cut(s)	2297
BstBI	TTCGAA	2 cut(s)	2464, 2552
BstC8I	GCNNGC	7 cut(s)	483, 1117, 1409, 1613, 2125, 2212, 2346
BstDSI	CCRYGG	2 cut(s)	2384, 2694
BstF5I	GGATG	8 cut(s)	119, 206, 408, 886, 901, 1099, 2155, 2308
BstH2I	RGCGCY	2 cut(s)	625, 2372
BstHHI	GCGC	2 cut(s)	624, 2371
BstMAI	GTCTC	8 cut(s)	197, 406, 848, 1070, 1770, 2385, 2741, 2783
BstMWI	GCNNNNNNNGC	8 cut(s)	452, 1304, 1313, 1490, 1499, 1608, 1934, 2264
BstNI	CCWGG	5 cut(s)	780, 1023, 1573, 2584, 2625
BstSCI	CCNGG	7 cut(s)	430, 431, 778, 1021, 1571, 2582, 2623
BstSFI	CTRYAG	2 cut(s)	834, 2356
BstV1I	GCAGC	5 cut(s)	154, 157, 1423, 1637, 2702
BstX2I	RGATCY	8 cut(s)	704, 1168, 1226, 1288, 1552, 1897, 2231, 2335
BstXI	CCANNNNNNTGG	2 cut(s)	423, 463
BstYI	RGATCY	8 cut(s)	704, 1168, 1226, 1288, 1552, 1897, 2231, 2335
Bsu36I	CCTNAGG	1 cut(s)	891
BsuRI	GGCC	6 cut(s)	354, 875, 1131, 1594, 1615, 2389
BtgI	CCRYGG	2 cut(s)	2384, 2694
BtgZI	GCGATG	1 cut(s)	1412
BtrI	CACGTC	1 cut(s)	871
BtsCI	GGATG	8 cut(s)	119, 206, 408, 886, 901, 1099, 2155, 2308
BtsIMutI	CAGTG	4 cut(s)	1372, 1851, 2493, 2665
Cac8I	GCNNGC	7 cut(s)	483, 1117, 1409, 1613, 2125, 2212, 2346
CaiI	CAGNNNCTG	1 cut(s)	1900
CciI	TCATGA	1 cut(s)	679
CfoI	GCGC	2 cut(s)	624, 2371
Cfr10I	RCCGGY	1 cut(s)	1407
Cfr13I	GGNCC	5 cut(s)	352, 723, 874, 1734, 2026
Cfr9I	CCCGGG	1 cut(s)	431
CseI	GACGC	4 cut(s)	108, 162, 474, 2771
CsiI	ACCWGGT	1 cut(s)	1571
Csp6I	GTAC	1 cut(s)	935
CspCI	CAANNNNNGTGG	2 cut(s)	2778, 2813
CviQI	GTAC	1 cut(s)	935
DriI	GACNNNNNGTC	1 cut(s)	948
EaeI	YGGCCR	2 cut(s)	1592, 2387
Eam1104I	CTCTTC	4 cut(s)	1314, 2152, 2580, 2850
Eam1105I	GACNNNNNGTC	1 cut(s)	948
EarI	CTCTTC	4 cut(s)	1314, 2152, 2580, 2850
EciI	GGCGGA	1 cut(s)	579
Eco130I	CCWWGG	4 cut(s)	289, 2384, 2405, 2694
Eco31I	GGTCTC	2 cut(s)	406, 1070
Eco47I	GGWCC	3 cut(s)	723, 1734, 2026
Eco47III	AGCGCT	2 cut(s)	623, 2370
Eco57I	CTGAAG	3 cut(s)	1031, 1515, 2733
Eco81I	CCTNAGG	1 cut(s)	891
Eco88I	CYCGRG	3 cut(s)	200, 431, 2669
EcoRII	CCWGG	5 cut(s)	778, 1021, 1571, 2582, 2623
EcoT14I	CCWWGG	4 cut(s)	289, 2384, 2405, 2694
ErhI	CCWWGG	4 cut(s)	289, 2384, 2405, 2694
Esp3I	CGTCTC	2 cut(s)	2741, 2783
FalI	AAGNNNNNCTT	6 cut(s)	1488, 1520, 2126, 2158, 2868, 2900
FaqI	GGGAC	6 cut(s)	448, 709, 944, 1012, 1437, 1747
FauI	CCCGC	1 cut(s)	131
FauNDI	CATATG	2 cut(s)	946, 2167
FbaI	TGATCA	2 cut(s)	439, 1030
FblI	GTMKAC	2 cut(s)	1755, 2900
Fnu4HI	GCNGC	5 cut(s)	168, 171, 1412, 1626, 2691
FokI	GGATG	8 cut(s)	106, 193, 415, 873, 908, 1106, 2162, 2295
Fsp4HI	GCNGC	5 cut(s)	168, 171, 1412, 1626, 2691
FspBI	CTAG	7 cut(s)	482, 1061, 1880, 1989, 2255, 2454, 2880
GlaI	GCGC	2 cut(s)	623, 2370
GluI	GCNGC	5 cut(s)	168, 171, 1412, 1626, 2691
GsaI	CCCAGC	1 cut(s)	60
GsuI	CTGGAG	2 cut(s)	2256, 2793
HaeII	RGCGCY	2 cut(s)	625, 2372
HaeIII	GGCC	6 cut(s)	354, 875, 1131, 1594, 1615, 2389
HapII	CCGG	2 cut(s)	432, 1408
HgaI	GACGC	4 cut(s)	108, 162, 474, 2771
HhaI	GCGC	2 cut(s)	624, 2371
Hin1I	GRCGYC	3 cut(s)	100, 1110, 2763
Hin6I	GCGC	2 cut(s)	622, 2369
HinP1I	GCGC	2 cut(s)	622, 2369
HincII	GTYRAC	3 cut(s)	718, 1923, 2871
HindII	GTYRAC	3 cut(s)	718, 1923, 2871
HindIII	AAGCTT	2 cut(s)	663, 674
HpaI	GTTAAC	1 cut(s)	1923
HpaII	CCGG	2 cut(s)	432, 1408
HphI	GGTGA	7 cut(s)	59, 878, 1159, 1933, 2639, 2778, 2833
Hpy99I	CGWCG	3 cut(s)	102, 108, 1115
HpyAV	CCTTC	9 cut(s)	485, 544, 1055, 1192, 1297, 1624, 1769, 2554, 2657
HpyCH4III	ACNGT	3 cut(s)	806, 1738, 2226
HpyCH4IV	ACGT	5 cut(s)	148, 870, 1110, 2601, 2734
HpyF10VI	GCNNNNNNNGC	8 cut(s)	452, 1304, 1313, 1490, 1499, 1608, 1934, 2264
HpySE526I	ACGT	5 cut(s)	148, 870, 1110, 2601, 2734
Hsp92I	GRCGYC	3 cut(s)	100, 1110, 2763
HspAI	GCGC	2 cut(s)	622, 2369
KroI	GCCGGC	1 cut(s)	1407
KroNI	GCCGGC	1 cut(s)	1409
Ksp22I	TGATCA	2 cut(s)	439, 1030
KspAI	GTTAAC	1 cut(s)	1923
LguI	GCTCTTC	2 cut(s)	1314, 2580
LmnI	GCTCC	3 cut(s)	1201, 2812, 2836
Lsp1109I	GCAGC	5 cut(s)	154, 157, 1423, 1637, 2702
LweI	GCATC	8 cut(s)	306, 468, 895, 1084, 1846, 2317, 2620, 2736
MabI	ACCWGGT	1 cut(s)	1571
MaeI	CTAG	7 cut(s)	482, 1061, 1880, 1989, 2255, 2454, 2880
MaeII	ACGT	5 cut(s)	148, 870, 1110, 2601, 2734
MaeIII	GTNAC	7 cut(s)	331, 367, 866, 902, 2116, 2183, 2326
MfeI	CAATTG	2 cut(s)	641, 930
MflI	RGATCY	8 cut(s)	704, 1168, 1226, 1288, 1552, 1897, 2231, 2335
MlsI	TGGCCA	2 cut(s)	1594, 2389
MluNI	TGGCCA	2 cut(s)	1594, 2389
MlyI	GAGTC	6 cut(s)	266, 546, 655, 838, 1772, 2661
MmeI	TCCRAC	4 cut(s)	1432, 2016, 2503, 2743
Mox20I	TGGCCA	2 cut(s)	1594, 2389
MroNI	GCCGGC	1 cut(s)	1407
MroXI	GAANNNNTTC	2 cut(s)	1634, 2138
MscI	TGGCCA	2 cut(s)	1594, 2389
Msp20I	TGGCCA	2 cut(s)	1594, 2389
MspA1I	CMGCKG	1 cut(s)	1414
MspCI	CTTAAG	1 cut(s)	2297
MspI	CCGG	2 cut(s)	432, 1408
MspR9I	CCNGG	7 cut(s)	432, 433, 780, 1023, 1573, 2584, 2625
MunI	CAATTG	2 cut(s)	641, 930
Mva1269I	GAATGC	2 cut(s)	1937, 1963
MvaI	CCWGG	5 cut(s)	780, 1023, 1573, 2584, 2625
MwoI	GCNNNNNNNGC	8 cut(s)	452, 1304, 1313, 1490, 1499, 1608, 1934, 2264
NaeI	GCCGGC	1 cut(s)	1409
NciI	CCSGG	2 cut(s)	432, 433
NcoI	CCATGG	2 cut(s)	2384, 2694
NdeI	CATATG	2 cut(s)	946, 2167
NgoMIV	GCCGGC	1 cut(s)	1407
NheI	GCTAGC	1 cut(s)	481
NlaIV	GGNNCC	8 cut(s)	568, 725, 777, 1203, 1290, 1554, 1735, 2493
NmuCI	GTSAC	4 cut(s)	367, 866, 2116, 2183
NspV	TTCGAA	2 cut(s)	2464, 2552
PaeR7I	CTCGAG	1 cut(s)	2669
PagI	TCATGA	1 cut(s)	679
PciSI	GCTCTTC	2 cut(s)	1314, 2580
PcsI	WCGNNNNNNNCGW	2 cut(s)	100, 2470
PctI	GAATGC	2 cut(s)	1937, 1963
PdiI	GCCGGC	1 cut(s)	1409
PdmI	GAANNNNTTC	2 cut(s)	1634, 2138
PfeI	GAWTC	4 cut(s)	4, 153, 2111, 2417
PflFI	GACNNNGTC	1 cut(s)	102
PkrI	GCNGC	5 cut(s)	169, 172, 1413, 1627, 2692
PleI	GAGTC	6 cut(s)	265, 545, 654, 838, 1772, 2661
PpsI	GAGTC	6 cut(s)	265, 545, 654, 838, 1772, 2661
PshBI	ATTAAT	1 cut(s)	110
PsiI	TTATAA	1 cut(s)	1772
Psp6I	CCWGG	5 cut(s)	778, 1021, 1571, 2582, 2623
PspFI	CCCAGC	1 cut(s)	56
PspGI	CCWGG	5 cut(s)	778, 1021, 1571, 2582, 2623
PspN4I	GGNNCC	8 cut(s)	568, 725, 777, 1203, 1290, 1554, 1735, 2493
PspPI	GGNCC	5 cut(s)	352, 723, 874, 1734, 2026
PspXI	VCTCGAGB	1 cut(s)	2669
PstNI	CAGNNNCTG	1 cut(s)	1900
PsuI	RGATCY	8 cut(s)	704, 1168, 1226, 1288, 1552, 1897, 2231, 2335
PsyI	GACNNNGTC	1 cut(s)	102
PvuII	CAGCTG	1 cut(s)	1414
RsaI	GTAC	1 cut(s)	936
RsaNI	GTAC	1 cut(s)	935
SapI	GCTCTTC	2 cut(s)	1314, 2580
SatI	GCNGC	5 cut(s)	168, 171, 1412, 1626, 2691
Sau96I	GGNCC	5 cut(s)	352, 723, 874, 1734, 2026
SchI	GAGTC	6 cut(s)	266, 546, 655, 838, 1772, 2661
ScrFI	CCNGG	7 cut(s)	432, 433, 780, 1023, 1573, 2584, 2625
SexAI	ACCWGGT	1 cut(s)	1571
SfaNI	GCATC	8 cut(s)	306, 468, 895, 1084, 1846, 2317, 2620, 2736
SfcI	CTRYAG	2 cut(s)	834, 2356
Sfr274I	CTCGAG	1 cut(s)	2669
SfuI	TTCGAA	2 cut(s)	2464, 2552
SinI	GGWCC	3 cut(s)	723, 1734, 2026
SlaI	CTCGAG	1 cut(s)	2669
SmaI	CCCGGG	1 cut(s)	433
SmlI	CTYRAG	8 cut(s)	374, 1172, 1505, 1517, 2030, 2297, 2669, 2894
SmoI	CTYRAG	8 cut(s)	374, 1172, 1505, 1517, 2030, 2297, 2669, 2894
SsiI	CCGC	5 cut(s)	124, 564, 1826, 2084, 2267
SspI	AATATT	2 cut(s)	767, 1387
SspMI	CTAG	7 cut(s)	482, 1061, 1880, 1989, 2255, 2454, 2880
StyD4I	CCNGG	7 cut(s)	430, 431, 778, 1021, 1571, 2582, 2623
StyI	CCWWGG	4 cut(s)	289, 2384, 2405, 2694
TaaI	ACNGT	3 cut(s)	806, 1738, 2226
TaiI	ACGT	5 cut(s)	151, 873, 1113, 2604, 2737
TaqI	TCGA	8 cut(s)	106, 391, 649, 660, 2221, 2464, 2552, 2670
TfiI	GAWTC	4 cut(s)	4, 153, 2111, 2417
TscAI	CASTG	4 cut(s)	1379, 1858, 2493, 2665
TseFI	GTSAC	4 cut(s)	367, 866, 2116, 2183
TseI	GCWGC	5 cut(s)	167, 170, 1411, 1625, 2690
Tsp45I	GTSAC	4 cut(s)	367, 866, 2116, 2183
TspDTI	ATGAA	5 cut(s)	419, 668, 1100, 1751, 1967
TspGWI	ACGGA	1 cut(s)	2301
TspMI	CCCGGG	1 cut(s)	431
TspRI	CASTG	4 cut(s)	1379, 1858, 2493, 2665
Tth111I	GACNNNGTC	1 cut(s)	102
Vha464I	CTTAAG	1 cut(s)	2297
VpaK11BI	GGWCC	3 cut(s)	723, 1734, 2026
VspI	ATTAAT	1 cut(s)	110
XapI	RAATTY	6 cut(s)	280, 730, 1724, 2017, 2395, 2437
XhoI	CTCGAG	1 cut(s)	2669
XmaI	CCCGGG	1 cut(s)	431
XmiI	GTMKAC	2 cut(s)	1755, 2900
XmnI	GAANNNNTTC	2 cut(s)	1634, 2138
XspI	CTAG	7 cut(s)	482, 1061, 1880, 1989, 2255, 2454, 2880
ZraI	GACGTC	1 cut(s)	1111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.